Human Mitochondrial DNA (mtDNA) Monitoring Primer Set
Contents
1. MightyAmp Thermal Cycler Dice SYBR Molecular Probes Inc DNA OFF ex Ex Tag http wwwr takara bio coJp 7 7246 TafdR4 Tel 077 543 6116 Fax 077 543 1977 http www takara bio coJp v201408Da2. mtDNA MightyAmp for Real Time SYBR9 Plus R075A B SYBR ex Ex Tag Ti RNaseH Plus RR820S A B 1 50 1 ND1 Primer Mix 10 uM each 1 50 ul 2 SLCO2B1 Primer Mix 10 uM each 2 50 ul 3 ND5 Primer Mix 10 uM each 1 50 ul 4 SERPINA1 Primer Mix 10 uM each 2 50 ul 1 DNA mtDNA 2 DNA nDNA NucleoSpin Tissue 740952 10 50 250 MightyAmp for Real Time SYBR Plus R075A B SYBR Premix Ex Tag Ti RNaseH Plus RR820S A B 0 2 ml 8 strip tube individual Flat Caps NJ600 1 8 0 2 ml Therma
3. VII NucleoSpin Tissue 740952 10 50 250 NucleoSpin Tissue XS 740901 10 50 250 MightyAmp for Real Time SYBR Plus R075A B SYBR Premix Ex Tag 1l Tli RNaseH Plus RR820S A B PCR Thermal Cycler Dice Real Time System TP900 TP960 Thermal Cycler Dice Real Time System Single TP850 TP870 Thermal Cycler Dice Real Time System Lite TP700 TP760 0 2 ml 8 strip tube individual Flat Caps NJ600 96well Hi Plate for Real Time NJ400 Sealing Film for Real Time NJ500 DNA OFF DNA 9036 VIII Yue Yuerg 2012 Hepatocyte like cells differentiated from human induced pluripotent stem cells Relevance to cellular therapies Stem Ce Research 9 196 207 IX
4. 1 1 gt MightyAmp for Real Time SYBR Plus 2X 12 5 ul 1 X SYBR Premix Ex Tag 1 Tli RNaseH Plus 2X Primer Mix 10 uM each 1ul 0 4 Meach Genomic DNA 5 ng 2 ul dH2O 9 5 ul Total 25 ul DNA 1 1 100 ng 10 ng NucleoSpin Tissue DNA OD2eo OD2so 2 MightyAmp for Real Time SYBR Plus gt 1 1 l 1 1 1 Hold Cycle 1 WK 98 C 2 3 Step PCR Cycle 40 98 10 60 15 68 30 Dissociation MightyAmp DNA Polymerase 98C 2 http www takara bio coJp 4 7246 lt SYBRe Ex 7ag Il Tli RNaseH Plus
5. DNA nDNA DNA mtDNA mtDNA DNA DNA OFF 9036 DNA PCR DNA OFF PCR DNA mtDNA NucleoSpin Tissue DNA PCR Primer Mix PCR mtDNA 2 nDNA
6. 246 IdfaR4 Human Mitochondrial DNA mtDNA Monitoring Primer Set vV201408Da Human Mitochondrial DNA mtDNA Monitoring Primer Set PCR DNA mtDNA DNA nDNA mtDNA nDNA 2 4 Pseudogenes DNA iPS 1 4 PCR mtDNA nDNA Gt mtDNA mtDNA iPS
7. EEE ET SLCO2B1 24 36 oO Nps ie SERPINA1 24 48 ND1 SLCO2B1 iPS 2 260 PS ND5 SERPINA1 ND1 SLCO2B1 FNN El 0 15 04 SERPINA1 24 51 700 600 500 J 400 y 200 200 100 0 iPS 1 iPS 2 DNA http www takara bio coJp 6 7246 VI RT PCR mtDNA DNA 1 100 ng DNA mtDNA NucleoSpin Tissue DNA DNA PCR
8. 2 PCR mtDNA nDNA Microsoft Offce Excel mtDNA URL http www takara bio coJp mtdna_monitoring_too http wwwr takara bio coJp 3 7246 PCR 1 DNA 4 4 Primer Mix 24 ul 1 gt Primer Mix 1 ul Thermal Cycler Dice Real Time System Thermal Cycler Dice Real Time System
9. gt Hotd 2 Seg PCR Hold Cycle 1 95 30 2 Step PCR Cycle 40 95 5 60 30 Dissociation 3 4 Ct mtDNA mtDNA nDNA mtDNA Microsoft Office Excel mtDNA Ct mtDNA 5 mtDNA a ND1 SLCO2B1 Ct ACt1 SLCO2B1 Ct ND1 Ct b ND5 SERP
10. INA1 Ct ACt2 SERPINA1 Ct ND5 Ct c ACt1 ACt2 2N N ACt d c 2 ND1 15 75 ACt1 8 78 440 SLCO2B1 24 53 ND5 15 04 ACt2 9 47 SERPINA1 24 51 1 URL http www takara bio coJp mtdna_monitoring_tool http www takara bio coJp 5 7246 V iPS 2 DNA mtDNA mtDNA mtDNA mtDNA IV iPS mtDNA mtDNA
11. l Cycler Dice Real Time System PCR Thermal Cycler Dice Real Time System TP900 TP960 Thermal Cycler Dice Real Time System Lite TP700 TP760 Thermal Cycler Dice Real Time System Single TP850 TP870 Applied Biosystems StepOnePlus Real Time PCR System Life Technologies Baseline Auto Threshold Manual http wwwr takara bio coJp 2 7246 lll 20 1 1 4 IV
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