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T4-RD1

1. 7 Fc 10 15 1mg mL 8 9 10 11
2. 5 200 L MSlgG1 RD1 200 uL 6 2 8CT 30 7 2 8 C 400 450 x g 5 8 3 4 9 PBS 200L 2 8 400 450 xg 5 10 3 4 11 PBS 200L EPICS Cytomics 3 6 1 2 Ficoll Paque
3. gt PBS PBS 2 8 C o NBS 2 BSA 0 1 0 1 T4 RD1 gt 1 5 L uL 195 uL PBS 1 N e gt al 6 PBS PBS 6603369 1 500mL pH 7 2 0 2 C 0 196 1 2 1 6603152 300 PBS
4. 3 4 LH 700 5 10x10 A mm 1 100L Ficoll Paque 1 4mL PBS 2 Ficoll Paque 4mL 1 3 2 8C 400 450x9 30 4 Ficoll Paque 5 2 lt 8 C PBS 2 8 400 450 xg 8 6 PBS 7 2 8
5. 3 RD1 PE Log 1 CD4 T4 RDI MSIgGI RDI CYTO TROL 6604248 Fc Fc MSlgG1 RD1
6. MSlgG1 RD1 200L 5 2 8 C 30 6 PBS 1mL 2 8 C 400 450xg 4 7 5 2 8 0 5 1mL PBS 9 EPICS Cytomics 3 Ficoll Paque 1 1 x 10 200 L Ficoll Paque UK 96 200 4L 2 2 8 C 400 450 x g 5 3 4
7. T U B ERE CHE TdT HLA DR Smlg T B T B T Pan T CD7 CD2 CD5 CD3 CD3 CD4 CD8 BRU CD1a CD4 CD8 CD2 CD3 CD5 CD7 CD4 CD8 T
8. AGC ERARE 3 CV 5 1 0 1 2 3 4 5 6
9. CD4 T T T B T CD4 class I MHC T4 SFCI12T4D11 CD4 ERARIO T BALB cJ TI ANDERE NS 1 AG4 lg IgGl H REO x L K RD1 Phycoerythrin PE RD1 fikv igk 0 5 1 5 486 575nm 568 lt 590nm 1 0 5mL E da aie xa 70 2 0 01M 0 15M 0 1 T
10. SLE CD4 CD8 SLE T4 T8 T4 T8 T4 T8 CD4 CD8 T4 T8 CD4 T4 CD4 T4 809 T 60 T4 4 6 B CD20 8510096
11. 1 2 8 2 T4 RD1 2 8C 3 20 25 C 4 T4 RD1 6602864 100 0 5mL 1 McMichael Differentiation Antigens Press D 202 206 2 Reinherz EL and Schlossman SF 1980 The differentiation and function of human T lymphocyles Cell 19 821 827 3 Aiuta F Cerottini J C Coombs RRA Cooper M Dickler HB Froland S Fudenberg HH Greaves MF Grey HM Kunkel HG Natvig J Preud homme J L Rabellino E Ritts RE Rowe DS Seligmann M Siegal FP Stjernsward J Terry WD and Wybran J 1975 Identification en
12. DT B f T lg T B E Smlg E T T Smig B lg Fc Ig Smg T B
13. T4 T8 CD4 CD8 MS SLE T4 T8 CD4 CD8 MS SLE CDA T4 T8 SLE CD8 TA T8 SLE CD4 CD8 SLE CD4 T4 T8 SLE
14. 6602864 2009 11 2007 2 20100EZY00113000 877429 T T4 RD1 4 _ IO 99 RD1 Phycoerythrin PE 1 T4 RD1 0 1 lt 1 0g T4 CD4 62kD CD4 8096 ERHO T 60 CD4
15. T CD4 1 6 488nm RD1 FS 907 SS 1 EDTA 6
16. SCID AIDS AIDS HIV HIV CD4 CD4 CD4 T4 T8 CD4 CD8 CD4 CD8 T4 T8 T T T4 T8 T4 T8 CD4 ARC AIDS related complex AIDS AIDS CD4 T4 T8 LEAS 0 CD8
17. 2 90 3 859 4 Ficol Paque Ficoll Paque 5 5 Ficoll Paque 6
18. T4 RD1 12 13 CD4 T4 T4 T4 CD4 T T CD4 gt CD4 14 15 16 AK ABET
19. b 12 25 000 1x 10 L 25L 13 Propidium lodide 0 01mg mL 10L 14 30 Acridine orange 0 005mg mL 10 u L 3 15 16 100 859 gt PBS PBS PBS 2 8 NBS 2 BSA 0 196
20. B pan B CD19 B pre pre B CD20 pre B CD19 CD20 B B B Eb B CD21 CD22 B B CD22 pre pre B CD22 T T CD4 CD8 2 T CD4 T4 CD4 T T4 CD4 DiGeorge
21. 129 29 T T Harvard Medical School Dr S F Schlossman Coulter Immunology T B SR 3 T B
22. 25 1mL MATE RL 30 2 250 gL 7 PBS 2mL 8 400 450xg 5 9 7 8 10 0 5 1mL PBS EPICS Cytomics 11 2 Ficoll Paque 1 12mm x75mm LET 2 Ficol Paque 1 x 108 48 4H 1x10 mm 1mL 3 2 8 CE 400 450xg 4 4 200 uL
23. 0 1 PBS PBS 0 1 RELET 2 lt 8 gt 1 1 2 100L 3 200wL MSlgG1 RD1 200L MAES 4 SCHELL Eig C 45 5 PBS 2 3mL MA THIEL 400 450 xg 5 6 a b a 2mL 5 15 b PBS
24. CC 400 450X g 4 8 PBS 9 2 8 CC 400 450X g 3 10 PBS 1 x10 mm 1 x 10 mL 11 a b 90 a 12 1x10 1x 103 mm 1mL 13 2 8 C PBS 2 8 C 400 450 x g 4 DH 14 0 05mg mL Propidium lodide 3 MATREL 1 15 2 8 C PBS 2 8 C 400 450 xg 4 16 15 17 8596
25. 24mL 1mL 9 2596 amp 8 2 1L 8 269 1 0g EDTA 4Na 2Na 37mg pH 7 2 7 4 1 1 2 30 H 2 10 15 20 30 MslgG1 RD1 T4 RD1 6602884 100 0 5mL Propidium lodide Calbiochem 53705 0 01mg mL 0 05mg mL Acridine o
26. lysis of human cyiotoxic T lymphocyies T4 and T8 effector T cells recognize products of different major histocompatibility regions Proc Natl Acad Sci USA 79 4395 4399 Reinherz EL Cooper MD and Schfossman SF 1981 Abnormalities of T cell maturation and regulation in human beings with immunodeficiency disorders J Clin Invest 68 699 705 Schmidt RE 1989 Monoclonal antibodies for diagnosis of immunodeficiencies Blut 59 200 206 Fauci AS 1988 The human immunodeficiency virus infectivity and mechanism of pathogenesis Science 239 617 622 Taylor MGJ Fahey JL Detels R and Giorgi JV 1989 CD4 percentage CD4 number and CD4 CD8 ratio in HIV infection How to choose and how to use J AIDS 2 114 124 Morimoto C Hafler DA Weiner HL Letvin NL Hagan M Daley J and Schlossman SF 1987 Selective loss of the suppressor inducer T cell subset in progressive multiple scferosis Analysis with anti 2H4 monocfonal antibody N Engl J Med 316 67 72 Reinherz EL Weiner HL Hauser SL Cohen JA Distaso JA and Schlossman SF 1980 Loss of suppressor T cells in active multiple sclerosis Analysis with monoclonal antibodies N Engl J Med 303 125 129 Weiner JL Hafler DA Fallis RJ Johnson D Ault KA and Hauser SL 1984 Altered blood T cell subsets in patients with multiple sclerosis J Neuroimmunol 6 115 121 Smolen JS Chused TM Leiserson WM Reeves JP Alling DW and Steinberg AD 1982 Heterogeneit
27. odgkin s lymphoma Phenotypic reconstitution and B cell function Blood 74 2203 2211 Reinherz EL Kung PC Goldstein G and Schlossman SF 1979 A monoclonal antibody with selective reactivity with functionally mature human thymocytes and all peripheral human T cells J Immunol 123 1312 1317 Reinherz EL Haynes BF Nadler LM and Bernstein ID eds Leukocyte Typing Il Human T Lymphocyies 1986 New York Springer Verlag p 8 9 5 6 27 Koepke JA and Landay AL Precision and accuracy of absolute lymphocyie counts Clin Immunol Immunopathol 1989 52 19 27 135 0063 5 7 TOC TEL 0120 566 730 FAX 03 5530 2460 135 0063 5 7 TOC Wa COULTER 6602864 0911 6 6
28. range Baker A366 3 0 005mg mL Ficoll Paque Pharmacia 17 0840 03 EDTA 1 1 100L 3 10 x 10 mm 10x 10 mm 3x10 mm PBS 20 25 6 a gt 10 x10 mm 10 20 x 103 2 20 30 x 10 3 30 40 x10 5 40 60 x10 6 60 100 x 10 10 100 200 x10 20 2 6 b lt 3x10 mm 1 25C 500 x g 5 2
29. umeration and isolation of B and T lymphocyies from human peripheral blood International Union of Immunological Societies IUIS Report July 1974 Clin Immunol and Immunopathol 3 584 597 4 Reinherz EL Haynes BF Nadler LM and Bernstein ID 1986 Leukocyle Typing ll NewYork Springer Verfag Vol2 p 8 15 20 37 5 Reinherz EL Meuer SC and Schlossman SF 1983 The delineation of antigen receptors on human T lymphocyles Immunol Today 4 5 8 6 Reinherz EL Morimoto C Fiizgerald KA Hussey RE Daley JF and Schlossman SF 1982 Heterogeneity of human T4 inducer T cells defined by a monoclonal antibody that delineates two functional subpopulations J Immunol 128 463 468 7 de Martini RM and Parker JW 1989 Immunologic alterations in Human immunodeficiency virus infection A review J Clin Lab Anal 3 56 70 8 Morimoto C Letvin NL Distaso JA Aldrich WR and Schfossman SF 1985 The isofatfon and characterization of AJ ed Leukocyie Typing Ill White Cell 1987 Oxford Oxford University 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 the human suppressor inducer T cell subset J Immunol 134 1508 1515 Morimoto C Letvin NL Distaso JA Brown HM and Schlossman SF 1986 The cellular basis for the induction of antigen specific T8 suppressor cells Eur J Immunol 16 198 204 Meuer SC Schlossman SF and Reinherz EL 1982 Clonal ana
30. y of immunoregulatory T cell subsets in systemic lupus erythematosus Am J Med 727 783 790 Smolen JS Morimoto C Stetnberg AD Wolf A Schlossman SF Steinberg RT Penne E Reinherz EL Rerchlin M and Chused TM 1985 Systemic lupus erythematosus delineation of subpopulations by clinical serologic and T cell maTker analysis Am J Med Sci 289 139 147 Morimoto C Reinherz EL Schlossman SF Shur PH Mills JA and Steinberg SD 1980 Alterations in immunoregulatory T cell subsets in active systemic lupus erythematosus J Clin Invest 66 1171 1174 Raziuddin S Nur MA and Alwabel AA 1989 Selective loss of the CD4 inducers of suppressor T cell subsets 2H4 in active systemic lupus erythematosus J Rheumatol 16 1315 1319 Sato K Miyasaka N Yamaoka K Okuda M Yata J and Nishioka K 1987 Quantitative detect of CD4 2H4 cefls in systemic lupus erythematosus and Sjogren s syndrome Arthritis Rheum 30 1407 1411 Ramos EL Turka LA Leggat JE Wood IG Milford EL and Carpenter CB 1989 Decrease in phenotypically defined T helper inducer celfs T4 4B4 and increase in T suppressor effector calls T8 2H4 in stable renal allograft recipients Transplantation 47 465 471 Pedrazzini AS Freedman AS Andersen J Heftin L Anderson K Takvorian R Canellos GP Whitman J Coral F Ritz J and Nadler LM 1989 Anti B cell monoclonal antibody purged autologous bone marrow transplantation for B cell non H

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