NKH1-RD1
Contents
1. 10 11 NKH1 RD1 12 13 14 CD56 NK NK T B 152. T B NK T B T lg NK NK I I MHC NK
3. 6 7 Fc 10 15 1mg mL 8 9
4. 6 2 90 3 85 4 Ficol Paque Ficoll Paque 5 5 Ficol Paque
5. gt PBS PBS PBS PBS 0 1 2 lt 8 gt 1 1 2 100wL 3 200L MSlgG1 RD1 200L 4 45 5 PBS 2 3mL 400 450 xg 5 6 a b
6. 16 gt PBS PBS 2 8 C NBS 2 BSA 0 196 0 1 gt 1 5L 5 L 195L PBS 1 PBS PBS 6603369 1 500mL pH 7 2 0 2
7. 3 B CD56 4 T B NK 3 CD3 T CD19 B CD56 1009 1009 T B NK null 3
8. 3 4 1 2 Ficoll Paque 3 RD1 PE Log CD56 NKH1 RD1 MSlgG1 RD1 3 1988 9 D scNKHL RD 16 1924 NKH1 RD1 3 1889 Coun HSIgG1 RD14 1 8 1624 SS 1 1899 TSIgG1 RD1
9. 0 196 2 1 2 1 6603152 300 PBS 24mL 1mL 9 2596 2 1L 8 26g 1 0g EDTA 4Na 2Na 37mg pH 7 2 7 4 1 1 2 30 2 10 15 20 30
10. CD56 y 1 10x 0 13 r 0 98 1 0 196 3 4 5 IM 4 4 6
11. a PBS 25 1mL 30 2 250 kL b 2mL 5 15 7 PBS 2mL 8 400 450 xg 5 9 7 8 10 0 5 1mL PBS 11 EPICS 2 Ficoll Paque 1
12. CFS CD3 CD56 NK CD56 NK NKH 1 CD56 TCR CD56 NK CD3 CD56 T CD3 CD56 CD56 T B CD56 3 9 6CV 59 55 CD56
13. 6603067 2009 11 2007 2 21100AMY00260000 877429 NK NKH1 RD1 IN ww RD1 Phycoerythrin PE 1 0 3 1gg NKH 1 CD56 135 220kD NKH 1 CD56 NCAM
14. 3 MslgG1 RD1 6602884 100 0 5mL 4 Propidium lodide Calpiochem 53705 0 01mg mL 0 05mg mL 5 Acridine orange Baker A366 3 0 005mg mL 6 Ficoll Paque Phamacia 17 0840 03 EDTA 1 1 100L 3 10 x 10 mm 10 x 10 mm 3x10 mm PBS 20 25 C 6 a gt 10 x10 mm
15. NK T B NK pan NK CD56 NKH 1 NK NK MHC CD56 NK MHC LAK Lymphokine acllvated Killer CD56 CD56 CD3 2 non T NK NK T MHC NK
16. 12mm x75mm 2 Ficoll Paque 1 x10 1x10 mm 1mL 2 8 C 400 450 xg 4 4 200L MSlgG1 RD1 200 L 5 2 8 C 30 6 PBS 1mL 2 8 C 400 450 x g 4 7 5 2 8 0 5 1mL PBS 9 EPICS 3 3 Ficoll Paque 1 1 x 10 200wL Fico
17. 10 20 x10 2 2 4 20 30 x10 3 30 40 x103 5 40 60 x10 6 60 100 x10 10 100 200 x103 20 b 3 x 10 mm 1 25 C 500 x g 5 2 3 4 LH 700 5 10x10 mm 1 100L 2 Ficoll Paque 1 4mL PBS Ficol Paque 4mL 1 3 2 8 C 400 lt 450 xg 30
18. 1 2 8 C 2 20 25 C 3 NKH1 RD1 6603067 100 0 5mL Knapp W et al ed 1989 Leukocyte a Typing IV Oxford Unlverslty Press pp 536 541 699 702 2 Schfossman SF et al ed 1995 Leukocyie a Typing V Oxford Unfversity Press Vol 1 p 270 vol 2 pp 1 398 1 400 3 Barclay AN et al ed 1993 The Leucocyie Antigen Facts Book Academfc Press pp 106 109 228 229 4 Robertson MJ and Ritz J 1990 Biology and clinical relevance of human natural killer cells Blood 76 2421 2438 5 Griffin JD et al 1983 Characterlzation of an antigen expressed by human natural killer cells J Immunol 130 2947 2951
19. NCAM 135 220kD NK 104kD TCR CD56 NK CD3 CD56 T CD3 CD56 CD56 TT B NKH 1 N901 CD56 BALB cJ NS 1 lg lgG1 H z L NKH1 RD1 RD1 Phycoerythrin PE RD1 0 5 1 5 486 lt 580nm 568 590nm 1 0 5mL
20. 0 2 0 01M 0 15M 0 19 NK 1 4 NK CD56 488nm RD1 4 FS SS 1 EDTA
21. IMMUNO TROL 6607077 Fc Fc MSlgG1 RD1 1 29 29 1 NKH1 RD1 2SD n 175 CD56 16 109 2 CD56 NK T CD3 CD56 T NK
22. 6 Hercend T et al 1985 Generation of monoclonal antlbodles to a human natural killer clone Characterization of two natural killer associated antigens NKH1A and NKH2 J Clin Invest 75 932 943 7 Shmidt RE et al 1986 A subset of natural kilfer cells in peripheral blood displays a mature T cell phenotype J Exp Med 164 351 356 8 Schmidt RE et al 1987 Enhancement of natural killer function through activation of the T11 E rosette receptor J Ciln Invest 79 305 308 9 Caliguri M et al 1987 Phenotype and functional deffcfency of natural kilfer cells in patients with chronfc fatigue syndrome J Immunol 139 3306 3313 10 10 Koepke JA and Landay AL 1989 Precislon and accuracy of absolute lyrnphocyte counts Clin Immunol Immunopathol 52 19 27 135 0063 5 7 TOC TEL 0120 566 730 FAX 03 5530 2460 135 0063 5 7 TOC Wa COULTER 6603067_0911
23. lodide 3 1 15 2 8 PBS 2 8C 400 lt 450 xq 4 16 15 17 85 b 12 25 000 1x10 1gL 25L 13 Propidium lodide 0 01mg mL 10xL 14 30 Acridine orange 0 005mg mL 10L 3 15 16 100 859
24. Ficol Paque 5 2 8C PBS 2 8 400 450 xg 8 6 PBS 7 2 8C 400 450 x g 4 8 PBS 9 2 8 C 400 450x9g 3 10 PBS 1 x10 mm 1 x 10 mL 11 a b 90 2 4 a 12 1x 10 1x10 nmm 1mL 13 2 8 C PBS 2 8 400 450 x g 4 14 0 05mg mL Propidium
25. l Paque U 96 200 gL 2 2 8 C 400 450 x g 5 3 4 5 200L MSlgG1 RD1 200L 6 2 8 C 30 7 2 8 C 400 450 x g 5 8 3 4 9 PBS 200L 2 8 C 400 450 x g 5 10 3 4 11 PBS 200L EPICS
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