2）尿中総イソチオシアネ－ト代謝物の分析
Contents
1. 1 2 1 3 2 365 nm HPLC 3 A R 1 mol 13 2 4 mol 1 E Dithiocarbamate N R R H R W 3 B A 2 1 mol M 1 mol 1 3 2 1 HPLC 32. mm 2 1 0 4mM 1 3 2 2 2 1 2 1 2 3 10mM 1 2 600uL 0 1M 500uL 4 2 100uL 5 65C 2
3. 1 Ri NH C S R gt S Ri 1 2 1 3 2 1 2 2 tert Butyl isothiocyanate H C C N C S 1 2 7 1
4. 2 mL J ki mm gt 1 N 1 2 2 21 21 1 2 HPLC HPLC 10 mM 1 2 1 2 28 4 mg 20 mL 2 0 1 M
5. 7 mm 2 56 5 51 2001 Johnson I T Glucosinolates Bioavailability and importance to health Tnt J Vitam Nutr Res 72 26 31 2002 Zhang Y Cancer preventive isothiocyanates Measurement of human exposure and mechanism of action Mutat Res 555 173 190 2004 Chung F L Jiao D Getahun S M and Yu M C A urinary biomarker for uptake of dietary isothiocyanates in humans Cancer Epidemiol Biomarkers Prev 7 103 108 1998 Zhang Y Cho C G Posner G H and Talalay P Spectroscopic quantitation of organic isothiocyanates by cyclocondensation with vicinal dithiols Anal Biochem 205 100 107 1992 Zhang Y Wade K L Prestera T and Talalay P Quantitative determination of isothiocyanates dithiocarbamates carbon disulfide and related thiocarbonyl compounds by cyclocondensation with 1 2 benzenedithiol Anal Biochem 239 160 167 1996 258
6. 1 36g 90 mL pH 8 5 100 mL 1 3 2 HPLC CAPCELL PAK C18 UG80 5 wm 4 6 mm x 250 mm 70 30 1 0 mL min 40 365 nm 5 4 Y 1 2 Benzenedithiol 65 2 HPLC 4 21 7
7. S R 120 p D Glucose Aglycone gt D Glucose B p Glucose Myrosinase i gt R N C S N N OSOs OSO Glucosinolate Aglycone Isothiocyanate 1 Mercapturic acid pathway 2 Glutathione transferase 7Y Glutamyltranspeptidase
8. Cysteinyl glycinase WW W Acetyltransferase WM W Acetylcysteine conjugate 19 21 7 mm 2 2 PE M Isothiocyanate R N C S GST R NH C S y GT R NH C S PS y Glu Cys Gly Cys Gly y Glu Cys Gy Glutathione R NH C S AT R NH C S Cys LAcetyl Cys Acetylcysteine conjugate GST Glutathione S transferase y GT y GlutamyItranspeptidase CG Cysteinylglycinase AT A Acetylransferase 2
9. 2 21 2 Jsothiocyanate R N C S 1 N C S Myrosinase Glucosinolate 1
10. SH S A R N C S a 8S R NH SH S 1 2 3 S SH R emsw CO Cg 1 pwr mr R SH S 4 2 3 1 Isothiocyanate 2 1 2 Benzenedithiol 3 1 3 Benzodithiole 2 thione 4 Dithiocarbamate 3 1 2 20 1 2 Benzenedithiol 21 1 2 N 1 2 1 3 2 1 3 Benzodithiole 2 thione HPLC MN Cyclocondensation assay 9 1 2
11. 6 7 6 HPLC 3 1 3 2 HPLC MV 1 2 1 3 2 HPLC 1 3 2 5 Tc00 1 3 Benzodithiole 2 thione 000 g00 UV 88 rm mAU 5 1 3 2 3 21 1 2
12. 3 2 1 HPLC 2 HPLC 1 2 Cyclocondensation assay i Fahey J W Zalcmann A T and Talalay P The chemical diversity and distribution of glucosinolates and isothiocyanates among plants Phytochemistry 24 21
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