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1. x ae Carl Zeiss LSM710 780 ZEN 1 1 1 1 1 1 1 1 2 Windows 1 1 3 1 1 4 Ar 1 1 5 NLO 1 1 12. Track Channels lt x Acquisition FT or Track1 Configuration Ex405 488 555 line Smart Setup Show manual tools a m Select all Unselect all New Auto Exposure Live Track1 w ww 405 458 488 514 561 594 633 486 nm 561 nm 594 nm Multidimensional Acquisition i Information On Experiment Integration Photon Counting 0 Fig 17 Channels _ a 1 0 Integration Photon Counting 0 000 1 00 7 Carl Zeiss LSM710 780 ZEN 1 OD a Bh tee Auto Exposure Master Gain Digital Offset Auto Exposure 1009
3. Show manual tools Smart SE Show manual tools New Auto Exposure Fig 14 Show manual tools Imaging Setup Light Path Imaging Setup Mode Channel Mode gt sequential Simultaneous Switch track every Frame r WV Track1 Rhod Sequential A488 Fig 15 Imaging Setup Carl Zeiss LSM710 780 ZEN Imaging Setup Light Path TE Light Path lt Imaging Setup Track Lambda Mode Light Path Track 2 Use Dye Color Detector Ch1 Alexa Fluor 568 Ch2 Reflecton Lasers T PMT gt Fig 16 Light Path Configuration
4. Fig 27 Fig 27 ZEN 2 1 Ism RAT Carl Zeiss ZEN Light Edition Windows OS http www microimaging zeiss co jp 2 Export
5. On Off Light Path ec Online Acquisition Fig 6 ZEN Workspace Configuration 2 Carl Zeiss LSM710 780 ZEN 1 2 3
6. MBS Vis InVis Ch1 Ch2 1nm PMT Dyes 10 Carl Zeiss LSM710 780 ZEN 2 Simultaneous Sequential 2 Simultaneous
7. Fastest Carl Zeiss LSM710 780 ZEN 4 2 Configuration Configuration Fig 13 Recent Configurations Search Ex 488 1 Ex 488 555 639 2 Load comigqurabon Acquisition Configuration Ex405 486 555 fine Smart Setup Show manual tools e ool 6 ew Auto Exposure Live Continuous Snap gy Z Stack e Time Series a amp Bleaching e Fig 12 Configuration Fig13 Configurations EE a
8. 27 3 Carl Zeiss LSM710 780 ZEN 10 1 1 Ocular 2 TFT HAL On Off ON 3 H 4 LD N WwW B WN Smart set up Configuration Light Path T PM
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10. Profile Spline Fig 46 Profile 8 10 Information 1 infonmatbon Fig 47 Information Carl Zeiss LSM710 780 ZEN 9 a0 Z r all era 3D Show all 9 1 Dimensions Z
11. Carl Zeiss LSM710 780 ZEN Microscope Objectives Objec Re Opto Light Home tives fector war path el ine Automat Load cic Js RL Illumination 5 Reflector Horne tyes Flector q var 4s 38 3 45 Load Ral osition Control Pos AUFomatic Pos 6 Analy DIC Trans light Contrast Manager a TL Illumination On Off RL Illumination On Off Light path 32 Carl Zeiss LSM710 7
12. x Acquisition Mode Objective Plan Apochromat 63x 1 40 Oil DIC M27 Scan Mode Frame v Frame Size X 512 Speed Pixel Dwell 1 60 psec ScanTime 983 04 msec Averaging Number 1 aa Fig 25 Acquisition Mode Line 4 Mode Line Frame Method Mean Sum Number Scan Area scan Area Show all Fig 26 Scan Area euc Size 134 7 pmx 134 7 pm Pixel Size 0 26 ym i 0 0
13. LED Power 9 10 LED LED Fig 1 1 1 5 NLO Chameleon Standby ON Chameleon ON 1 2 fa ZEN 2010 ERE ES DIY ILC FRL FAL TV IFES SE Start System Login ZEN 2010 LSM 780 gt Boot Status Image Processing Fig 2 Start System
14. 3D 3D 1 Z Stack 2 3 4 Z Z Stack a ps Auto Exposure Live Continuous Snap Z Stack 21 Slice S Bleaching Z Stack First Last en gt Start Experiment 1 2 3 4 Fig 34 Application Center Live ores apie sh ange terva 1 000 pum 13 37 pm
15. Et Fam ane ry i Lens LSM Fig 8 Axio Observer Z1 Fig 9 Goniigquration el DsRed er a Assign TFT De Il Fig 9 Carl Zeiss LSM710 780 ZEN 4 Offline Acquisition Fluorescence Shutter On Shutter Off 4 1 Smart Setup a Smart Setup ae Configuration Ex405 488 555 fine Config
16. Master Gain Digital Offset Digital Offset 0 Lasers 488nm 1 59 14 Carl Zeiss LSM710 780 ZEN Range Indicator Master Gain Digital Offset Range Indicator Dimensions
17. Contents of image window Ortho Full resolution image window Z single plane 1 series Movie 1 Compress Carl Zeiss LSM710 780 ZEN 6 2 File Open New File Browser New File Browser
18. Live Continuous ee 13 Carl Zeiss LSM710 780 ZEN Merge Dimension Merge On Off Fig 20 Split 6 Channels Channels Showal i Trackt Select all Unselect all Track1 Laser Y wv 405 458 488 514 561 594 633 488 nm Lasers A 561nm 594 nm Gain Master Digital Offset Digital Gain Fig 21
19. 10 15 Carl Zeiss LSM710 780 ZEN 3 Ocular Online Fluorescence Shutter On Shutter Of ee Y LE DY DLEF On Off DIC Ill 1
20. Image Processing Image Processing Boot status Fig3 Carl Zeiss LSM710 780 ZEN ZEN 3 Fig 3 Fig 3 3 Fig 3 ZEN
21. i J ASR Coniquesian EEE bee ey im il Ce Fig 18 Auto Exposure Fig 19 Live Continuous Auto Exposure Master Gain Z Master Gain Continuous 1 Averaging
22. Fig 50 Player 28 Carl Zeiss LSM710 780 ZEN 9 4 Overlay Wi Ww i ie ri a y Delete Font _ Cut region Coordinate Fig 51 Overlay QD Angle Delete Cut region Coordinate Z Hide Number Measure 4G
23. LAL Sequential 11 Carl Zeiss LSM710 780 ZEN 5 aan live ee Channels Track 1 Airy Unit Fig 17
24. Keey Interval Slice Set First Z Fig 35 Z stack Set First 206 3 2 Set Last Z wo Stop 3 Carl Zeiss LSM710 780 ZEN Optimal Zack Showal First Last Center Optimal amp Slice Optical Slice Fig 36 Optical Start Experiment Z Averaging a
25. Fig 21 5 Carl Zeiss LSM710 780 ZEN Acquisition Mode lt x Acquisition x Acquistion Mode Configuration Ex405 488 555 line ne Objective Plan Apochromat 63x 1 40 Oi DIC M27 Scan Mode Frame Frame Size x 512 speed Pxel Dwell 1 60 psec ScanTime 4963 04 msec Multidimensional Acquisition i Information On Experiment Averaging Number BitDepth 6 Bit Fig 24 Acquisition Mode Frame Size 512x512 Optimal Optimal Speed
26. Ef 1 vv vr vv HM one Reset All Fig 26 Scan Area Carl Zeiss LSM710 780 ZEN IE 13 Live Live 18 Carl Zeiss LSM710 780 ZEN 6 6 1 2D
27. LSM D 20 Carl Zeiss LSM710 780 ZEN 2 Export TIFF JPEG File Ti ee Export Channel Contents of image window single plane Q Select file Cotens ot age dons name and save Save Full resolution image window ser Fig 30 Export Format Data Raw data
28. 4 2 Setup Manager Laser ON ON OFF st i te Ao A 0PS5 561 10 Wane fenton EE EE Pee ITU PO 50 imi Wavelength 635 hm Status FEW Fig 7 Blue Diode 405 405nm COMPONENTS ON Argon 458 488 5 14nm Page 2 DPSS 561nm HeNe 543nm 594nm 633nm ON NLO MaiTai Clameleon ON NLO Laser Properties
29. 2 3 4 HBO Field Stop Aperture Stop FL P amp C 0 N DO UU BP MD 36 Carl Zeiss LSM710 780 ZEN 11 OFF 11 1 OFF DPSS 561nm HeNe 543nm 594nm 633nm NLO Setup Manager Laser Off Chameleon NLO
30. Fig 48 Dimension 3ch Zoom Merge Single Ch on off Channel s On Off Crop PUTA Reuse 9 2 Display me OTT GET 100 50 a 10 00 1 00 2 Fig 49 Display 9 3 Player Z EER Peer Options
31. New Auto Exposure Live Continuous Snap Z Stack 21 Slice s Time Series Bleaching Regions 4 Start Experiment Fig 37 Application Z 8 24 Carl Zeiss LSM710 780 ZEN 8 2D 3D 4D A 8 1 2D Fig 38 2D 2 Z 20 3D Time Series A Mode Dimension Player 1 8 2 Split Split 2 ER
32. 2 Ism Carl Zeiss LSM710 780 ZEN 1 Ism Fig 28 New Image File Save Save as Fig 28 Fig 29 peichern unter Speicten Destoo a ef E gt Eigene Dateien demo data Arbeitsplatz JenaKulur a Netzwerkumgebung JofBioTech_CallforPapers CiscoSecure Authentication Agent L5M 4 2 Icons 5 Divx Movies Movies 2 axiovision 4 6 Icons Music Dateiname Image2 Date Fig 29 LSM Ism LSM Save CD
33. DIC I 40 DIC I 40 1 LSM 35 Carl Zeiss LSM710 780 ZEN 10 3 1 Ph1 lt 3 1 2 10 4 ON 1
34. Dimension Merge On Off Fig 39 Split 8 3 Gallery 3D 4D A Gallery Gallery Z Stack Time Series Fig 40 Gallery 25 Carl Zeiss LSM710 780 ZEN 8 4 Ortho Z a Ortho Contents Export Fig 41 Ortho 8 5 Cut Z Cut Fig 42 Cut 8 6 3D Z 3
35. ovI 29 Carl Zeiss LSM710 780 ZEN 9 5 Series 3D 3D a 3D 3D Fig 52 3D Series X Y 1 Render series X Y 2 Total frames 3 360 Difference angle Panorama HH Senes 1 Fender med Tamaroundx gt ai ee Total frames 20 i 1 4 6 2 50 100 Difference angle 10 Paar First angle a a i 3 Fig 53 Apply 6 Player Player
36. i a 7 Stack 3 3D 9 5 Series Fig 43 3D 8 7 Histo Histogram Fig 44 Histogram IG Carl Zeiss LSM710 780 ZEN 8 8 Co localization 2 Co localization aa Fig 45 Co localization 8 9 Profile
37. Main Switch ON ON SYSTEM PC COMPONENTS ON SYSTEM PC PC COMPONENTS PC SYSTEM PC DA ON 1 1 2 ON PC ON Windows LSM User 1 1 3 ON ON Carl Zeiss LSM710 780 ZEN 1 1 4 Ar Ar Power Enable ON 1 5 idle run
38. Speed Pixel Dwell Scan Time 10 8 9 7 Bit Depth Bit Depth 16bit 65 536 12bit 4 096 8bit 256 16 Carl Zeiss LSM710 780 ZEN Averaging Averaging Fig 25
39. Ch iin Range Indicator Dimensions Zoom Merged Ch111 Ch2 T11 Ch1 12 x eee Fig 22 Dimensions Range Indicator 0 Master Gain Digital Offset 1Airy Fig 17 Master Gain 800 1000 Fig 21 Channel
40. Chameleon ON Standby Argon 458 488 514nm idle 0 5 Ar OFF x Ar 11 6 Main Switch OFF 11 6 11 2 OFF 11 3 11 4 ZEN File Exit 11 5 Windows Shut Down PC OFF 11 6 SYSTEM PC COMPONENTS OFF 2 Main Switch OFF
41. ZEN 30 24 ZEN Show manual tools Show manual tools Setup Manager Light Path Fig 4 Fig 4 Show manual tools Carl Zeiss LSM710 780 ZEN Show all J Showal Show all Fig 5 Show all
42. File New File Browser 2 Acquisition Maintain Macro Took View Window Help Fig 31 File Menu Q File Browser HDD Fig 32 New File Browser Reuse New File Browser Leo lt gt a os Cut ml Copy Re use 9 Revse 22 Carl 7 Zeiss LSM710 780 ZEN 3D Z
43. 9 3 30 Carl Zeiss LSM710 780 ZEN 10 Axio Observer Z1 Axio Observer Z1 6 6 TFT
44. 28 10 31 11 37 1 2 3 n 859 15
45. 80 ZEN 1 2 3 L 4 5 6 7 8 1 2 9 10 25mm 11 12 13 LM set 14 XY 15 16 L 17 18 On Off 2 3200 19 On Off 20 TET 21 22 WARS TE 23 24 25 26
46. Microscopy from Carl Zeiss LSM 710 and LSM 780 LSM Software ZEN 2010 February 2010 We make it visible Carl Zeiss LSM710 780 ZEN ZEN2010 ZEN tt 1 1 OO 2 2 6 3 7 A 8 5 12 6 19 7 3D 23 8 weetustcnvnusveciee s ce cvaieneepinveseyeswcwueoxteestasaenndesevieedeadeleukootwseenadconst 25 9
47. T 10 Fig 16 Gain Fig 16 Light Path 34 Carl Zeiss LSM710 780 ZEN 10 2 200um DIC DIC 1 2 0 PC 2 DIC TD DIC
48. ure your experiment Search Dyes a Recent Fig 10 Acquisition RE rhodamine Alexa Fluor 594 Alexa Fluor 568 Search Dyes Alexa Fluor 700 Fig 11 Smart Setup 5 Fastest Best Signal Best Compromise 3 Apply Fastest Simultaneous Best Signal Sequential Fastest Best Compromise Sequential
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