モリナガ FASPEK 特定原材料 ウエスタンブロットキット 操作マニュアル
Contents
1. 142. 3 10 S RG 0 2 EID 0 AP A OO lgG I 1 Laemmli Sample Buffer 2 19 1 Laemmli Sample Buffer SDS 2 A A B B C 1 1 1 17
3. 15 MW 50 000 MW 38 000 MW 33 000 35 000 8B MW 18 400 x g mL 1 x g mL 8
4. IV 1 1 1 4 2 1 3 5 3 4 30 3 5 20 3 3 13 6 100 mM Tris pH9 5 15 3 10 7
5. FASPEK 8 1 2 1
6. 1cm 10 1 2 3 2 11 30 4 15 V 60 38 kDa 31 kDa 201 kDa 125 kDa
7. ys 9 E 100 10 VECTASTAIN ABC AP Rabbit IgG kit VECTOR IgG 10 000 11 VECTASTAIN ABC AP Rabbit IgG kit A 10 mL 1 10 mL B 1 30 12 Alkaline Phosphatase Substrate Kit IV lt BCIP NBT gt VECTOR 1 100 mM Tris pH9 5 10 mL
8. FASPEK H A 1 B F A A TT 3 1 2 1 g 50 mL 19 mL 3 12 90 110 pH
9. E 1 1 4 1 Y 3 3 30 Y 3 20 Y S 3 Y 100 mM Tris pH9 5 15 Y
10. E 2 3 E 3 1 0 100mL I IT 60 mA gel Eg 15V 1 IV
11. pH 6 0 8 0 pH 5 3 000 xg 20 6 1 1 2 5 4 1 2 1 1 2 35 2 D 10 7 gmL 1 gmL 0 5 g mL 1 1g gmL 5
12. 2 10 mL 2 3 2 1 20 mL IL 1 2 3 7 L 10 gmL 1 g mL 0 5 gp gmL 20 L 4 x400 400 1 60mA
13. 2010 2 2 FASPEK 236 0003 2 1 16 2001 4 5 2008 6 3 7 ELISA T
14. Tris BES 10x TEFCO 10 1 500mL 6 10XTris Glycine BIO RAD 1 2 7 1 150mL 7 10XTBS BIO RAD 10 Tween 20 0 05 1 1 000mL 8 0 1
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