「KOD FX Neo」を用いた短鎖および長鎖ターゲット のマルチプレックス
Contents
1. PCR KOD FX Neo 7kb 10kb KOD FX Neo PCHRH PCRH KOD FX Neo PCR PCRH PCR 50ng ul Human genomic DNA 5 I PCH PCR PCR 1 000bp 10kbp 5kbp gt 500bpp B PCR B Multiplex PCR C PCR B2. PCRH PCR KOD FX Neo PCR PCR 1kb PCR KOD FX Neo 1kb 10kb PCR PCR DMD BMD PCRH KOD FX Neo PCRH PCR PCR Alan H Beggs et al Human Genetics 1990 86 45 48 PmF 535bp Exon3 410bp Exon6 202bp Exon13 238bp Exon43 357bp Exon47 181bp Exon49 439Dp Exon50 271bp Exon60 139bp PCR PCR 1kb 10kb
3. PCR KOD FX Neo PCR PCR PCR Chrome9 1kb MSH6 1 8kb BRCA2 2 25kb WT 1 2 5kb FANCE 3kb RAD51D 4kb KRAS 5kb BRCAT 7kb DDB2 10kb PCR DW 7 PCR PCR xBuffer for KOD FX Neo 25 Ll 98C min 98C min mM dNTPs 10 Al Primer mix primer 2 5 pmol ul 6 ll 98C 10sec 98C 10sec Ce 50 ng ul Human genomic DNA 1 ul 60C 30sec 30 cycles 68C 10min KOD FX Neo T al 68C Tmin total 50 ul 4C 4C Ke7 2014 102 7 PCR PCR 1 000bpp 10kbp 5kbp gt 500bpp 1kb Ladder KOD FX Neo M 100bp Ladder A Taq PCR 1 KOD FX Neo B Long Range PCR 0 2 A Tag PCR 0 B PCR 3 B PCR C PCR 4 B Multiplex PCR B Multiplex PCR PCR KOD FX Neo
4. KOD FX Neo PCR PCR 1 PCR PCR PCR PCR KOD FX Neo KOD FX Neo
5. Multiplex PCH M 1kb Ladder 5 KOD FX Neo M 100bp Ladder 6 A Taq PCR 100bpp 1 KOD FX Neo 7 B Long Range PCR 2 A Taq PCR 1kbp 8 B PCR 3 9 4 0 KOD FX Neo PCR KOD FX Neo PCRH KOD FX Neo PCR KOD FX Neo PCR KOD FX Neo KOD FX Neo S00Ux1 200 P0C KFX 0 1 35 000 KOD FX Neo 1 0U ul XPCR Buffer for KUD FX Neo 200UX1
6. X5 1 000 0 KFX 201X5 140 000 mM dNTP 200UX1 X10 2 000 20 KFX 201X10 260 000 50I 901JKe7 8 2014 102
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