【課題】醸造用麹の製造法において原料の盛込み水分が 通常よりも低
Contents
1. HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH Con0885_h 160 HHHHHHHHHHHHHHHHHHHHDNAHHHHHH 100000 Aspergillus sojael DNA O 0 ATCC42251 Aspergillus soj ae Sakaguchi et Yanadal 0 5nmjHH HH r pnt 0 0000 O Wzard Genom c DNA Purification Kit promegal DNA HHHHHHHHHHHHHHHHHHHH 0 PCRD NAHHH DNAQ 20u gl PCRH HHHHHHHHHHH PCRI Con0885 h 1601 000000 l U D 0 0 D 02. o o o o E N m in C soos C C ne rernm rr pjo D m C o z o go Soo 3 Zoo oo L3 EE mo a L3 N oo ge 3 ES ss C Oo ae E L3 L3 o B on _ 20 IX E EJ n E zs o z 3 C G G ooo C3 o C 1 o BL 1 ooo C os L3 z Oo KA Ed D E C mo X 3 3 E DO is VEL m I m E ete E T oog ad S Z Sve E C Ej E eh L3 G G GE a Lee a Ew C oO 3 3 m S E S nl 0 0 oo Ep S C ot di GB E LIEBER ne Bo OI ri C L3 C o EES PA Q exe 7 MES EYE S TA 3 EXE 3 Ot re C oo C oOo a e pw EM jaa re
3. 2004 290142 JP 2006 340641 A 2006 12 21 3 2004 290155 10 117771 10 20 IU U U U U U 0 D 000 D 00 0 D 00 0 D D 0 0 D 0 0 As 30 50 RNAi HHHHHHHHHHHHHH pergillus soj ae 4 JP 2006 340641 A 2006 12 21
4. 2006340641000001 app 10 72 JP 2006 340641 A 2006 12 21 72 0 0 U l U U U U U U 0 U U U HHHH UUU U U 48024 A405 BAIL QO2 DAL GA1 HAI2 48063 Q17 QO Q7 Q5 OH R32 48065 AA60X AA60Y ABO1 ACO2 ACO BAOL CA42 G34 QOl BB19 BE23 BB26
5. HHHHHHHHHHHHHHHHH MOlecular Cloning A Labor at ory Manual 2 editiong 0 0 0 0 O00 O Cold Spring Harbor Laboratory Press SBN 0 8769 309 6 Current Protocols in Molecular BiologyH 7 0 0 0 0 0 D John Wely amp Sons Inc ISBN 0 471 50338 XT 000000000000000 DNA HHHHHHHHHHHHHHHHHHHHH U L DO00000000000000000000000 DNAU O DNAD HH 10 o Ooo uud DNA lO OU ce IL L U DNAD DNAHHHHHHH treBHH
6. l U U U U 0 0 0 0 DU 0 0 0 0 0 0 0 0 0 0 U l DU D 0 0 D D 0 0 50 JP 2006 340641 A 2006 12 21
7. m EN c x o c C z rae Q EX ED 3 S nm gcc zs o F i G c C G EL NES y Pee Eli 3 ai E C m ESS n ts G RN C Sr mn 9 Sve O b C C 2 EX ES C f ERE Se Hte a EY st O 3 3 oO 3 a P a oO 3 3 oO m L3 a a mn 3 r3 lt A 4 oO c ex Ow E a 7 JP 2006 340641 A 2006 12 21 E ec e No M C und nm 0o H H H H H H H H H uH H H LAP T D 0 0 D 0 D 0 0 D 0 0 MM D 0 0 D 0 D 0 0
8. 2 50 25 protease activity amp LAP activity ta SEN E E protease U g 8 5 MLAPmQU gD TR lt mg e 50 35 2 JP 2006 340641 A 2006 12 21 U U DD 0 EP E EE 00 0 0 Hl 10 U U U IlHHHHHHHHHH U L U U U U U U U U U U 20 U HH OO U U U U U U U U U U U U U uuu HHHHH U U U U 30 n IO UU L 40 2004 290142 0000009 10 117771
9. EMBL3 Stratagene DNAHHHHHH 30 uu jiugauggbspPauuumguguuuguuukiajunmudluectuleultJeMroiSr XL Blue Stratagene DMMrrison 0 Methods in Enz vno ogy 68 326 331 19790 D TD TT 0 D O O O O0 O O O0 O O0 D D T DH O O0 O 00O 0 0 0 B Ho hn Methods in Enzynology 68 299 309 1979 00000000000000 igngggamug9gggugumgggsuuagusuuuumsuuuUuuuuuusrlmtll 0 OO DAT H O O0 D O O0 O0 D O 0 D O0 Li COR MODEL 4200 40 LH 370DNAH 00 00 000000 000000000 CEQ2000XL DNA J U U U U U U U U U U 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 l U U U U U U U U U U U U U U U U U U U U U U U U U U l U DUD DD 0 DD 0 0 0 D EB AERE D D U Aspergillus 0 0 0 D 00 0 0 D 0 0 0 0 D 00 D D 0 0 0
10. 19 JP 51 Int Cl C12N 1 14 2006 01 C720 1 68 2006 01 C12N 15 09 2006 01 21 2005 168129 P2005 168129 22 17 6 8 2005 6 8 64 520 HHHHHHHHHHHHHHH HHHHHHHHHHHHHHHHHHHH U HHHHHHHHHHHHHHHHHHHH U U U U IHHHHHHHHHH IHHHHHHHHH l U U U l U U U l U DD D 0000 U IHHHHHHHHHH IHHHHHHHHH IHHHHHHHHHHHHHHHHHHHHHH l U U U IHHHHHHHHHHHHHHHH OOOD 1 14 1 68 15 00 15 00 J P 2006 340641 A 2006 12 21 11 2006 340641 P2006 340641A 43 18 12 21 2006 12 21 A 4B024 A 4B063 ZNAA 4BO65 A RAR 6 OL 10 71 74 72 72 72 000004477 2 50 100100181 FRET 2 50 lm 399 Zo
11. D pH l U D 0 0 D 0 Ul D 0 0 D 0 0 0 D 0 0 0 0 0 0 0 D 0 0 D 0 0 D 0 U lD 0 MLe uci ne p nitroanilide0 0000 Cn D 0 0 D 0 0 D 0 0 0 0 D 0 0 u ln D 0 U m nm U lli LR h 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Eh 0 0 UU Uo no OH p nitoroaniline LAPH O l U U U U U U U U U U U U U U U U U U U U U U U U U U U U U U U U U U U D 0 D 00 0 0 0 0 0 D 0 D 0 0 O0 0 0 0 00 0 0 D 0 0 0 0 DO 0 0 0 LA SJ 90 479 1 205 6 protease U g LAP mU g 4 SJ 35 192 1 treBA 35 276 9 124 7 U U SJ treBA m LU X mHHHHHHHHHHH CHHHHHHH C nig00000000000
12. 0 D 0 0 D 0 0 D 0 0 D 0 0 D 0 0 D 0 DONA 00 U PCRI O O GeneAnp5700 DNA detection system Perkin El mer DNA PCRH 95 C309 O 56 C30 72C2H 300 0 2 5kbf DNAD D DNAD O0 0 0 7 0 O O0 O O O O 0000 QAquick Gel Extraction K t QI AGE l O D 0 O0 D O0 0 D O O0 D O O D O D D O 0 DNA TOPO TA Cloning Kit Invitrogenl pCR TOPO OG H D U LH Bi gDye Term nator v3 1 Cycle sequencing Kit U O0 D O0 DD 0 D 0 0 D O0 O0 D 0 D AB DNA 0 PRI SM 3770 D 0 000000 0 D 0 0 D 0 020002000000000 l U U U U U U U U U U U U U U U U De 700002 5kb LU Con0885 h 160 0 U U Takahashi 0 0 0 O Mol Gen Genom cs 2004 272 344 352 2001 460537 B LU U U U 2001 460530 0000000 pMWPX 20 Pstl Sph 0 0 0 D D 0 D D 0 0 0 0 D D NA l D D Pacl Sse8387I Fs el Noti Fsel Sse83871 pyr G T pyr G PCRJ D 0 D D 0 DD 0 0 TA 0 0 D OD Fsel Sse83871 U U U gd treB BDugg agagagaaguauaugausuauugsuudtH tl LU D O D 0 O D O 0 D O DAT O0 0O O0 PCRO D 0 0 D O0 0 O 0 PCRI Il C CD 0 D D 0 0 C0 DD 0 D D 0 D D 0 D 0 0 D 0 0 D 0 0 D 0 0 D 0 0 0 0 O0 TOPO TA cloning KitH 0 PCRH HHHHHHHHHHH 10000 N
13. 00000000000000000000 C0 0 U nnnnnnmH eC ee E Res eC nnn E T e treBA E E Miu dog nn t z D F H AER R a a TT PC OO RL R EK H E 1 HHHHHHHHHHHHHHHHHHHHHH 8 JP 2006 340641 A 2006 12 21 1 3 o es 2 ood LR treB A E 04 02 i Oh 05h th 15h 2h 25h 3h a e e c e Le SR ET ge 20 Z K protease U g amp 8 ig BLADE 15 8 la mR mg g EN 2 e e D o gt protease activity amp LAP activity SJ treB A SJ treB A 50 35 9 JP 2006 340641 A 2006 12 21 cfu Dmin 30min 60min 90min 120min time 50 C
14. HHHHHHHHHHHHHHHHH 0 ATCC42251 Aspergillus soj ae Sakaguchi et Yamada HHHHHHHHHHHHHHHHHHHHHHDNAHHHHHHH DNAD 20 IUDOO0OO0O00000000000D0PcSRUODOODOOOODOOODODOOO DNA U D D O0 0 D D D O0 D O 0 D 0 O DNAD O D O O PCR O 0 0 DAQ DM H H H D D D 1 0 0 OD DNA TIDMA DD 0 TA Cloning Kit Invitrogen pUC119H 0000000 pBR 3227 0 0 0 pBluescript SKH Stratagenel DNA
15. ot Fsel Sse8387 10 Pacl 0 D 0 0 D 0 0 D D 0 D D 0 0 D 0 0 U U pUC19 M13 forward M13 revers eU U D 0 0 D 0 D 0 0 D 0 0 D 0 0 D 0 0 D 0 ATCC42251 pyrG 10 20 30 50 JP 2006 340641 A 2006 12 21 6
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