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1. SLG 550nm SLU 580nm SLR 630nm 3 MultiReporter Assay System Tripluc SLG SLO SLR SLG SLO SLR pSLG SLO SLR SV40 control pSLG HSVtk control lt2. Yeast two hybrid system P 1 Products MultiSPRinter SPR MultiSPRinter SPR MuItiSPRinter
3. 3 MultiReporter Assay System Tripluc SV40 HSVtk 5 18 1 1 1 0 pSLG test Code No MRV 101 2 1 1 pSLR test Code No MRV 103 pSLG test Code No MRV 101 3 2 1 pSLO test Code No MRV 1 02 pSLR test Code No MRV 1 03 TA SLO TA Cloning Kit Code No MRT 101 KOD
4. Web URL http www toyobo co jp bio 100 400 Powerpoint Word JPG TIF 5 10 000
5. Assay Toxi Blocker Toxi BlockerTM Toxi Blocker M 1 Toxi BlockerW Neuro a Neuro 2a Lipofectamine 2000 Invitrogen gal plasmid 6 Toxi BlockerW gal Toxi Blocker Toxi Blocker 3
6. SPRH I H i ee ae en Me ee Xr Photo linker QH Dummy linker Small molecule Photo linker OD 1 Ee Dummy linker N vvNN NT gt 1 gi 96 96
7. 20 CLX 101 1 850 000 6
8. 1 2 2 3 MTA Material Transfer Agreement http www asterand com NEW RELEASE Toxi Blocker Transfection Supplement A OO Toxi BlOocker Toxi Block GUupplement Toxi BlockerW 1
9. http Www toyobO CO jpD DiO TOYOBO 2006 2007 2006 2007 4 17 4 3 4 0 Vol 1 Santa Cruz 006 Santa Cruz GO06 9 13 http www toyobo co jp bio gt 1 1616 5 Lloyd S
10. 0b EE PKCa 84 1 384 824 PKAC _ wrab RO318220 GF109203X 125 5 TAMRA rs a CooH OF IM Kinase a gt gt Tryphostin51 Tryphostin47 0 2 75 TryphostinAG1288 IF R0318220 Phosphatase lt 50 25 GF109203X QTL Sensor fluorescent HH i 0 Stawosporine te 10 20 30 40 50 60 70 80 1 compound QTL M 6 490 600nm TAMRA QTL
11. Toxi Blocker it 9 1 14 A 2 a i 8 Toxi Blocker 2 ga 3 Toxi Blocker Transfection Supplement TFP 10 1 000 3 200 2006 4 20 2006 5 19 409 o0ff 17 MultiReporter Assay System Tripluc
12. te Fluor 3 F HiLyte Fluor 3 i I HiLyte Fluor AnaSpec Cy Alexa Fluor 488 Cy 3 Alexa Fluor 555 Cy 5 Alexa Fluor 647 Cy 5 5 Alexa Fluor 680 Cy 7 Alexa Fluor 750 HiLyte Flu
13. lt gt HiLyte Fluor Labeled Secondary Antibody Sampler kit Goat 1 Mouse Rabbit i x a anti mouse and Goat anti rabbit IgG Highy Cross adsorbed Label HiLyte Fluor 488 555 647 6XS50 g 20C AS72001 50 19 000 HiLyte Fluor Labeled Secondary Antibody Sampler Kit 1 Mouse Goat Rabbit anti mause and Rabit anti goat IgG Label HiLyte Fluor 488 555 647 SXSOLE 20C AS7EUOU 50 HiLyte Fluor Labeled Secondary Antibody Sampler Kit 1 Mouse Rabbit Goat anti mouse and Goat anti rabbit lgG Label HiLyte Fluor 488 555 647 2OC AS7EOUE 5U 19 000 Can Get Signal inmunostain Starter Set 5 Solution AA Solution BB mlXx 1 4e NKB 40 1 12 000 Can Get 5 g77g immunostain Starter Set Solution A Solution B
14. KOD Dash 50UX 1 o0C LDP 101 o5 000 TArget Clone 10 MagExtractor PCR amp Gel Clean up 00 Mag ical Trapper 1 TAK 101 12 000 NPK 60 1 5 000 MGS 101 38 000 4 Asterand 40 10 2004 HE RNA FFPE RNA DNA
15. Bait 5 Bait LexA Prey HA 3 N C NubG Prey N ek _ ER Lumen 1 a C CuD N Nub
16. 30sec 1kb PCHRH PCR 1 TA TArget CloneW pTA Vector 4 5kb PCH PCR JM 1098 Blend TaqW KOD Dash 5 196 PCR 1 Primer F primer TCGAGGTCGACGGTATCGAT 20mer GC 5596 R primer CGCTCTAGAACTAGTGGATC 20mer GC 5096 Blend Tag D W 10xBuffer for Blend Taq 2mM dNTPs 1 21 9 Forward primer 10pmole gl Reverse primer 10pmole gl Blend lage Total KOD Dash D W 1
17. SLG SLR SLR SLG SLO 3 SL0 SLR SLG SLG short life 26 TA cs 2 MultiReporter Assay System Tripluc
18. ratio AP1 SLO NFkB SLR oN RO NO TNFa ng ml ratio Oo NM RO OD NO AP 1 SLO NFkB SLR 1 7 8 453 455 2005 Biotechniques 38 891 894 005 CodeNo_ pSLG test 20 g 20C MRV 101 80 000 pSLO test 20 g 20C MRV 102 80 000 pSLR test MRV 103 80 000 SV40 pSLG SV40 control MRV 201 80 000 SV40 pSLO SV40 control MRV 202 80 000 SV40 pSLR SV40 control MRV 203 80 000 HSVtk pSLG HSVtk control MRV 301 80 000 MultiReporter Assay System Tripluc jn Detection Reagents Lysis Solution Assay Reagent MultiReporter Assay System Tripluc jn WV D Iuciferin MRA 101 MRA 10 MRA 103 MRL 101 MRHL 10 100 00 10 000 30 000 99 000 1 850 000 4 000 92 000
19. Neurosci Lett 336 121 2003 J B C 266 15391 1991 AKT PKB Rac protein kinase Km 3 9M AKT Histone H2B Ki 12M J B C 275 36108 2000 Annu Rev Biochem 68 965 1999 Glycogen synthase kinase 3 AKT1 PKBa AKT2 PKBb AKT3 PKBg MAPKAP kinase 1 p70S6K AKT B B C 293 1191 2002 J B C 272 30491 1997 Annu Rev Biochem 68 965 1999 CDK1 J Biochemistry 42 849 56 2003 Cell 112 243 56 2003 Histone H1 peptide CDK5 Km 40M Simian virus 40 large T antigen p34cdc2 Km 74xM CaM kinase I Casein kinase I I Glycogen synthase kinase 3 Phosphorylase b kinase PKA PKC Ty
20. NubC LexA VP16 Cub Al NubC LexA VP16 Ag b N C Bait Nucleus Prey a Bait Pex11 LTranscription ON Preys 8 od od HIS3 ADE2 8 gal Cub Pex1 1 His Protein protein interaction 3 1a 1 Proc Natl Acad Sci USA 1082 20 7121 7126 2005 2 J Biol Chem 280 24 22917 22924 2005 pBT 3 N bait vector for typell baits BLE 2OC DSsSPOS3S230 109 000 PpBT3 C bait vector DSP03G 31 109 000 pBT3 SUC bait Vector for typeI baits DSP 0 108 000 pBT3 STE bait Vector for type I DaitS Without signal sedquence DSP0 108 000 pPR3 N prey vector DSP0G 4 108 000 pPR3 prey vector pPR3 SUC prey vector for typelI preys BLE DSP0 DSP0G
21. 10 1 4kb 0 5 kD 0 1kb KOD plus PCR SLR TA Cloning Kit for KOD SLR pSLG SV40 control Jurkat Detection Reagents SLG Upload80 P 13 14 PSP SNS NN ASS GS No SNS oN AT_ QA _Q eee RS OR SE YS 9 EN RR Ee NN YY 3 pSLO test pSLR test SV40 AP 1 NF HSVtk S5LG HeLa S3 4 3 Detection Reagents 1
22. 1 96 18x 18mm MuItiSPHRintereAu Surface Plasmon Resonance Imaging System MultiSPRHinter 96 986 1 G 6 SPR MultiSPRintere AA 1 SPR CCD EE 9 A
23. PCR M 1 2 3 4 5 6 7 8 1 000bp 500bp Crassostres gigas A 1 4 Crassostres gigas B 5 6 TO0bp Blend Tag 0 050U 10 gl M 1 3 Lane M 100Dp Lane 1 Blend Taq Lane 2 Blend Tag Plus Lane 3 A ES RT PCR M M S 6 A 0 125U 10l 7 A 0 050U 10 l 8 6 3 A 0 025U 1 0 1 3 Lane 1 Blend TaqW Plus Lane 2 Blend Tag Lane 3 A F4 ek 1 ek4 4Blend Taq KOD Dash PCR Blend Taq KOD Dash PCR
24. Blend Taq Lane 1 RI Ja Lane 2 DNA 5ng Taq NA PCR Taq went NRN Ne PCR TA MO ES Blend Taq Taq 3 4 4 High Fidelity KOD KOD Plus KOD Plus Ver 2 Blend Taq PCR http www toyobo CO jD bio Barns W M Proc Natl Acad Sci USA 91 2216 2220 1994 Blend Tag PC1 RT PCR
25. 5x10 2x108clones ug DNA 2 2 Lane 1 DNA Lane 5 g carrier DNA Lane 3 5 Lane 4 2ug carrier DNA A 1 Carrier DNA DS Yeast transformation kit kit P50C DSPO01003 39 500 Single stranded carrier DNA mlx3 P0C DSPO600 1 000 5 http Www dualsystems Com QTL Pro d ucts BIOSYSTEMS QTL LightSpeed Universal Assay Development Kit TAMRA 1
26. 3 6kb 2 10kDb dNTP 0 3mM Target Number Segment Of 1 kb 6kb Cycles 94C min Denaturation oC Primer Tm 5C 55 60C 3 Annealing 30 30sec 58 72C 72C Tmin kb PCR target 4 Extension 1 min 1mn kb PCR target 1 98C 5 10 6kD Tm 70C 1 Human globin human myosin heavy chain DNA100ng 00ng PCR Blend Taq Blend Taq Plus
27. 2 3 FACS ELISA IHC HyLite Fluor 2 1 FT HiLyte Fluor 647 A mouse monoclonal anti 8 amyloid 1 11 antibody Code No HiLyte Fluor AS29547 rabbit anti Tau antibody cocktail Code NN No AS2958
28. Gal4 ha Activating domain 9 lt Nucleus Bait E Bait 5 N LexA DNA Cc ex MR NR 1 NA wr SI Promoter Reporter gene 8 anecriniion on LexA RT Bait LU Bait NN 9 a HIS3 ADE2 LexA DBD NN Bait a gal 3 1 DUALhybrid system 1 J Biol Chem 879 16 16503 16510 2004 0 000 0 000 0 000 0 000 pLexA N bait vector 5u8g 1 DSP03105 pLexA Dait vector 5u8g 1 DSPO3106 pPGAD HA prey vector 5Lg 1 DSPO3103 pY3H bridging vector 5Lg 1 DSPO3104 DUALhybrid control plasmid St DLexA p593 control Dait DLexA lamin C Control bail pACT large T control prey 1set 5 g 1 DSPO310 0 000 NMY5 yeast reporter strain MATa his3 A200trip 90 Tleu2 3 112 adepLYS lexAop 4 HIS3ura 1 DSsSP0o4005 lexAop 8 lacZ ade lexAop 8 ADE2 GAL4 lt gt DUALhybrid Kit DLexA N pLexA C pGAD HA pLexA p53 pLexA lamin C pACT large 1 NMY5 DUALhybrid system Y H
29. 6 Dualsystems Yeast two hyDrid system Yeast two hybrid Yeast two hyDbrid 3 2 LexA VP16 3 HIS3 ADE2 lacZ NMY51 Bait ER SUC STEO N
30. TA TA PCR TA SLO SLR TA Cloning Kit SLO SLR TA Cloning Kit for KDD TA KOD PCH TA 1
31. 96 Lysis Solution Assay Reagent 96 Upload 2 GS oO MultiReporter Assay System Tripluce pSLG SV40 Code No MRV 201 pSLG HSVtk Code No MRV 301 gt D luciferin 3 in vo Luciferase Proteins etc Lysis Solution Assay Reagent WW 1 4 in o Ne 5 in ro 4 19 1 2
32. Y 2H DUALmembrane DUALhybrid system 1 96 deep well OD s gt 2 1 aa HI 3 A6is 1 HTX beta galactosidase assay kit 965 20C DSP01002 39 500 DS Yeast transformation Kit Y H DUALmembrane DUALhybrid system 3 DNA
33. 450nm lt 490nm gt 600nm 490nm QO N C PC Staurosporin 5 TAMRA RFAI 1 Proc Mg Acad Sci USA 101 43 15295 15300 2004 QTL LightSpeed Universal Assay Development Kit 400 GO Non phosphorylated peptide Control Phosphorylated peptide control 800 GO Post reaction Duffer 10xXSensor Sensor Dilution Buffer 384 gt 4 3 DMSO0 www qtlDio Com QTKO25KO004 180 000 QTKO25KOO08 pr 5 10 3 CREBtide Kemptide PKA PKC MAPKAP K1 Substrate PKC Subs
34. 23 0kbD 1 Blend Taq Blend TaqW Plus ge M 1 3 4 5 6 7 8 M Blend Taq Blend Taq Plus A Lane M 1kD DNA Lane 1 8B globin T 3kb Lane 5 Myosin h c 6 2kb Lane 2 8B globin kD Lane 6 gloDin 8 5kb a _ Lane 3 B globin 3 6kb Lane 7 8 globin 17 5kb 1 Lane 4 Myosin h c 5 2kb Lane 8 globin 23 0kb 4 DNA _ BlendTad BlendTaqY Pluse A Human 8 globin 3 6kb II Blend TaqW Blend Taq Plus DNA 5ng Lane M 1kD DNA
35. Detection Reagents Lysis Solution Assay Reagent En EE 5 mmi 1 1 MultiReporter Assay System Tripluce 93 1 1 SLG SLO SLR
36. Pol I Pol I C Blend Taq Blend Taq Plus Taq DNA Blend Taqg Plus Taq PCR 13 PCR mixture D W de io WI 10XBuffer for Blend Taq BS mM dNTPs 5 Primer F 10uM Primer R 10 M Template DNA 5 200ng 4 Blend Tag 2 5units ul 0 5 Total 5Ogl PCR G6kb
37. 23 10 xKOD Dash buffer 3 2mM dNTPs 3 Forward primer 10pmole gl 0 35 Reverse Drimer 10pmole 0 35 KOD Dashe 0 3 Total 30 ul Blend Taq 55C 4 5min KOD DashS 60C 8 5min X 30 Cycles 94C 94 C A C 1min 1kb Tm 5 10 C x 30cycles 94C 94 C 72C SeC B sec A C 30sec 1 kb Tm Tm 5 4min 30sec 30 1 1 PCR 15 PCR MagExtractor PCR amp Gel Clean up kit Blend Taq KOD Dash M 1234M12 3 4 4 5kb Lane M Tkb Lane 1 4 3 PCH 0 4 3 PCH
38. US5 283 1 73 US5 468 6 1 115 000 DSPO1004 168 000 8 Ef 4 a DUAL Libraries 0C DSPO2203 455 000 0C DSPO2213 455 000 DUALn rane Yeast two hybrid system prey gt DSP0 15 455 000 DSP0 16 455 000 DSP0 05 455 000 Human adult Drain cDNA library NubG x Human mbryonal brain cDNA library NubG x 100 ugx2 100 ugx2 100 ugx2 Human adult liver cDNA library NubG x 100 ugx2 Jurkat T cDNA library NubG x 100 ugx2 HeLa cell line cDNA library NubG x 100 ugx2 DSP0 1 455 000 Mouse adult heart cDNA Iibrary NubG x 100 ugx2 DSsSPO2207 455 000 Mouse adult brain cDINA Iibrary NubG x 100 ugx2 DSsPO2201 455 000 100 ugx2 DsPo2202 455 000 Drosophila melanogaster whole adult cDNA library NubG x 100 ugx2 DSP0 14 455 000 Arabidopsis thaliana CDINA Iibrary NubG x 100 ugx2 DSP0 210 455 000 Human adult kidney cDNA library x NubG 100 ugx2 DSsPOo2208 455 000 Human adult liver cDNA library x NubG 100 ugx2 DsPo2209 455 000 Mouse adult heart cDNA library x NubG 100 ugx2 DSsSPO2206 455 000 Human a
39. Ca 17calmodulin dependent protein kinase CaMK CaMK I autoinhibitory domain CaMK I pseudosubstrate core CaMK II PKC myosin light chain kinase Biophys J 72 554 1997 Biochemistry 32 1032 1993 Ce 112 77 2003 J B C 274 3485 1999 J B C 274 3485 95 1999 J B C 221 581 93 1994 Pharmacol Exp Ther 283 885 1997 J B C 258 11391 1983 Brain Res 962 41 2003 Brain Res 851 54 1999 J Am J Physiol 274 E708 1998 J Appl Physiol 88 352 2000 J B C 274 26199 1999 Neurosci Lett 336 121 5 2003 B B R C 215 861 7 1995 Arch Biochem Biophys 252 418 25 1987 CaMK I Km 7 5uM Ca calmodulin Ca Brain Res 962 41 2003 J B C 265 17613 1990 CaMK I Native CaMK I Km 136gzM Km 54 3 M
40. PCR PCR Blend Taqg KOD Dash Taq DNA 3 5 Exonuclease DNA DNA 3 5 Exonuclease KOD _ DNA KOD DNA DNA KDD DNA Taq DNA 19 000 550U Blend Taq Plus
41. SLO TA Cloning Kit for KOD Code No MRT 201 SLR TA Cloning Kit Code No MRT 102 KOD SLR TA Cloning Kit for KOD Code No MRT 202 SLO TA Cloning Kit Code No MRT 101 SLO TA Cloning Kit for KOD Code No MRT 201 SLO TA Cloning Kit Code No MRT 101 SLR TA Cloning Kit Code No MRT 102 KOD SLO TA Cloning Kit for KOD Code No MRT 201 SLR TA Cloning Kit for KOD Code No MRT 202 pSLG SV40 Code No MRV 201 pSLG HSVtk Code No MRV 301 pSLG test pSLR SV40 Code No MRV 203 1 SV40 2 HSVtk SV40 EE i I 1 in vivo
42. 10 5 000 Report of research SDS PAGE O XX SDS PAGE mM IL SDS Dye 1 SDS PAGE TOYOBO 3
43. SS 8 1 SNAP 1 SNAP cell a SNAP tag cell W 505kit SNAP cell W430kit CHO IN SNAP tag 600 i 9 7 1 SNAP vitro M gt 3 3 SNAP vitro E SNAP vitro Emission ES 6 5 60min SNAP cell 430 Starter Kit Ex 4 1nm Em 444 484 nm Blue laser filter lt gt CVSKOZ 100 000 S SNAP cell 505 Starter Kit Ex 504 nm Em 532 nm fluorescein filter
44. Yeast two hyDbrid Three hybrid Y 5H 1 kit 1 DSPO9901 641 500 LexA DNA binding domain DBD C Gal4 AD N Bait prey Bait LexA Thre hVDrid Prey HA pYdH bridge Bait Prey EE 2m 3 HIS3 ADE2 lacZ NMY51
45. D Iluciferin vitro ATTO in o MultiReporter Assay System Tripluc Detection Reagent Lysis Solution Assay Reagent
46. k CHa H ch a Ho 5 a Aldosterone ALD Hydrocortisone HCN Bisphenol A bisA CH Ch CH CH diethylstilbestrol DES Testosterone TSS _ OCH CH NCH Tamoxifen TXF 4H Tamoxifen 4HTXF ERa 60 nM SPR signal Time sec 0 600 1200 1800 E RB 80 nM 25 20 XxF EST 15 4HTXF DES 10 bisA TSS HCN 0 ALD 0 600 1200 1800 Time sec 6 8 ER 1 Brockman J M Fruto A G and Com R M J Am Chem Soc 181 8044 8051 1999 2 Nelson B P Frutos A G Brockman J M Corn R M Anal Chem 1 18 3928 3934 1999 3 Brockman J M Nelson B P and Corn R M Annu ev Phys Chem 51 41 63 2000 4 Kanoh N Kyo M Inamori K Ando A Asami A Nakao A Osada H Anal Chem in press 2006 MultiSPRinter 12 3 Surface Plasmon Resonance Imaging System MuItiSPRinter SPR SPR 2031 110000 SPR 101 16 000 000 MultiSPRinter SPR SPR 10 12 000 000 MulItiSPRinter SPR
47. 6 108 000 108 000 pPR3 STE prey Vector for type lI preyS Without signal sequence BLUE DSPO3237 108 000 Vector control set lset 5ug pCOCCW Alg5 pAI Alg5 DDL Alg5 EB DSPO3238 TO9 000 NMY5 yeast reporter strain MATa his3A 0O0trip 901leu2 3 112 adeoLYS lexAop 4 HIS3ura DSPO4005 115 000 lexAop 8 lacZ ade2 lexAop 8 ADE2 GAL4 lt gt DUALmembrane Kit 3 DpBTS N pBTd C pBT3 SUC pBT3 STE pPRSd N pPR3 C pPR3 SUC kit 1 DSP01001 572 500 pPR3 STE pCCW Alg5 pAl Alg5 DDL2 Alg5 NMY5 DUALmembDbrane starter package DUALmembrane kit 3 HTX beta galactosidase assay kit DS Yeast transformation kit DUAL membrane Western blotting control extracts bait extract prey eXtract 50 IX DSPO0600 6 000 Lex A 2 1g lt LexA lgG1 gt Santa Cruz 200 g ml SASC7544 54 000 DUALmembrane kit 3 DUALmembrane starter package Dualsystems Biotech DUALhyDrid Yeast two hybrid system
48. HiLyte Fluor 555 conjugated goat anti mouse IgG Code NO AS61057 H555 HiLyte Fluor 488 COnjugated goat anti raDDit IgG Code No AS61056 Relative Intensity ww 1 1 1 1 1 H488 650 675 700 Wavelength nm 1 1 HiLyte Fluor 647 goat anti rabbit IgG 4 A goat anti rabbit IgGs eb re In 3 A 4 B Patrick L NN ANNM NcGeer gt Unconjugated AS2817 AS28176 12 000 AS28164 AS28168 14 000 AS29709 14 000 Biotinylated AS28175 AS28179 13 000 AS28167 AS28171 13 000 AS29718 14 000 HiLyte Fluor 488 labeled 500 LE AS28175 H488 AS28172 H488 13 000 AS28164 H488 AS28168 H488 13 000 AS29709 H488 14 000 HiLyte Fluor 555 labeled 5OO LE AS28175 H555 AS28172 H555 13 000 AS28164 H555 AS28168 H555 13 000 AS29709 H555 14 000 HiLyte Fluor 647 labeled 5O00 LE AS28175 H647 AS281782 H647 13 000 AS28164 H647 AS28168 H647 13 000 AS29709 H647 14 000 FAM TAMRA AMCA FITC
49. SNAP tag GeneCopoeia cDNA SNAP tag 1 6 000 DRF N C SNAP tag CMV T7 SNAP Capture Resin S Code No CVSK048 OmicsLink SNAP tag ORF Expression Clones www genecopoeia com UI lt hi rodu Tag Protein Labeling Kits
50. Ao Biol Cell 14 302 2003 J B C 276 7366 2001 B B R C 198 898 1994 Cell Mol Biol Res 40 383 1994 J B C 278 4474 2003 Arch Biochem Biophys 325 289 1994 B B R C 198 898 1994 J Cell Physiol 194 13 2003 Clin Cancer Res 9 516S 2003 Int J Pept Protein Res 45 587 1995 EGFR protein tyrosine kinase CaMK Cell 80 225 1995 Nature 373 573 1995 pp60src J Cell Physiol 194 13 2003 Clin Cancer Res 9 516S 2003 Int J Pept Protein Res 45 587 1995 Src family p60c src Km 55uzM p34cdc2 cdc2 6 20 KVEKIGEGTYGVVYK 6 4 Int J Pept Protein Res 45 587 92 1995 Harvey Lect 94 81 119 1998 Insulin receptor INSR Eur J Biochem 226 525 1994 4 ek 1 ek4 CodeNo Blend Tad 50Units X BTQ 101T 5 000 50Units X 1 BTQ 101 19 000 3 en O a 1 000Units x 1 BTQ 102 6a2000 1 000Un
51. 50 55 60 65 6 SPR CCD SPR SPR SPR 6 A A 4 an De Hr HO HO HO Cyclosporin A CSA Digitoxin DTN 0 O OH a cH CH OH oH 2 H 1 cu Ho 1 er a Ta a CH H Ey 6 JO 1 HO HO 4 Digoxgenin DXH Beta Estradiol EST Corticosterone CTS CSA i DIG i DTN 2 SS SS Sieo eo OS 1 Gb bp lt ES DXH EST CTS Cyclophilin A Anti DIG ee lt mw Anti CSA
52. lt gt CVSKOO3 1 95 000 SNAP cell TMR Star Starter Kit Ex 554 nm Em 580 nm rhodamine filter lt gt CVSK038 1 95 000 SNAP vitro 488 Starter Kit Ex 506 nm Em 526 nm fluorescein filter CVSKOO05 75 000 SNAP vitro 532 Starter Kit Ex 536 nm Em 554 nm rhodamine filter CVSKOO7 75 000 SNAP vitro 547 Starter Kit Ex 554 nm Em 568nm TAMRA or Cy3 filter CVSKO4 1 75 000 SNAP vitro 600 Starter Kit Ex 606nm Em 626 nm Texas red filter CVSKOTI3 80 000 SNAP vitro 632 Starter Kit Ex 637nm Em 657 nm 635 nm diode laser CVSKO15 85 000 SNAP vitro 647 Starter Kit Ex 660 nm Em 673 nm 650 nm diode laser CVSKO431 85 000 SNAP vitro 732 Starter Kit Ex 732 nm Em 747 nm Cy7 filter CVSKO 4 95 000 SNAP vitro Starter Kit Ex 752 nm Em 763 nm Cy7 filter CVSKOZ6 95 000 3 4 3 DMSO SNAP tag www covalys Com P d UU C S Expressway to Discovery OmicsLink SNAP tag ORF Expression Clones GeneCopoeia OmicsLink clone
53. 103 4 500 000 MultiSPRinter Au MultiSPRinter NHs C MultiSPRinter COO0H 1 C SPR 202 20 000 MultiSPRinter 96 1 80 4 nil SPR 200 SPR 201 20 000 6 000 SPR 401 29 000 ai N SNAP cell 430 SNAP vitro 732 747 2 MM Human 0 alkylguanine DNA alkyItransferase hAGT 3 60kDa N C SNAP cell In vitro 2 SNAP vitro 3 6 fo a SNAP cell
54. 6082 5 5mg protein 5 5mg protein 5 5mg protein 39 000 39 000 39 000 AnaTag HiLyte Fluor TR Protein Labeling Kit AnaTag HiLyte Fluor 555 Protein Labeling Kit AS Z1006 AS Z100 9 1 6 3 568 39 000 59 000 5 5mg protein 5 5mg protein AnaTag HiLyte Fluor 647 Protein Labeling Kit 652 669 5 5mg protein AS Z1008 59 000 AnaTag Biotin Protein Labeling Kit HiLyte Fluor 488 acid Succinimidyl eSter HiLyte Fluor 555 acid Succinimidyl ester 4 3 DMSO AS71003 5 5mg protein 39 000 i 502 527 5mg AS81161 59 000 552 569 2OC AS81251 59 000 HiLyte Fluor 647 acid Succinimidyl ester 649 674 2OC AS81256 29 000 HiLyte Fluor 680 acid succinimidyl ester HiLyte Fluor 750 acid Succinimidyl ester 678 699 2O0C AS81261 39 000 754 778 1mg AS81266 39 000 rodu I Labeled Secondary Antibodies HiLyte Fluor 1
55. Alamada California 94501
56. Anti EST EF L 1 Anti CTS Cyclosporin A CSA Cyclophilin A x 3 8 4 6 ER 8 5 ERC ER SPR MultiSPHintere ER 24 4H 6 CH CH C C 2 5 ER gt Beta Estradiol EST HO O C CH OH OH CH
57. CREB protein PKA Km 16M PKA 40S ribosome S6 231 239 PKA PKC p90rsk kinase MAPKAP K1 PKC Nucleic Acids Res 20 6106 1992 Insect Biochem Mol Biol 32 505 2002 Eur J Biochem 252 245 1998 Proc Natl Acad Sci U S A 83 5968 1986 FEBS Lett 175 219 1984 J Biochemistry 42 849 2003 J B C 278 319 2003 J B C 277 45451 2002 J B C 272 952 1997 PKC Hepatitis C virus HCV 3 1487 1500 PKC J B C 381 19 27 2000 J B C 274 30722 8 1999 PKC8 Km 22 9M 431 Thr eEF 1a 422 443 Arg429 PKCS 277 41850 2002 J B C 270 6156 1995 PKCg Km 68M JB C J B C 266 14390 1
58. Register Quality Assurance LRQA ISO0 9001 Restriction Endonucleases Modifying Enzymes IS0 14001 JAB JIC Quality Assurance Ltd IS014001 IS014001 Oc RI TE No E004 QS Accreditation R 016 N JAB QS Accreditation RE 002 NOTICE TO PURCHASER L
59. 991 J B C 265 7301 1990 FEBS Lett 243 351 1989 Myristoylated alanine rich C kinase substrate MARCKS protein kinase C PKCz PKC8 e atypical PKC Pharmacol Ther 93 271 2002 J B C 272 4072 1997 J B C 268 1501 1993 CaMK Glycogen synthase Ca2 calmodulin dependent protein kinase TI CaMK TI PKC PKC Km 4 1M Biochem J 268 303 1990 C 262 772 1987 J C 260 14471 1985 MAPK PKC neurogranin PKC PKC Km 150nM PKA CaMK I PKG PKG la Km 226M PKG I Km 84M PKG PKG IT Km 305M PKG la Km 59M cGMP specific phosphodiesterase CcG BPDE PKA Km 320M PKG Km 68M PKC CaM kinase 1
60. IMITED LICENSE A license under U S Patents 4 683 202 4 683 195 4 965 188 and 5 075 216 or theirforeign counterparts owned by Hoffmann La Hoche Inc and F Hoffmann La Hoche Ltd Roche has an up front fee component and a running royalty component The purchase price of this product includes Imited nontransferable rights under the running royalty Component to use Only this amount of the product to practice the Polymerase Chain Reaction PCR and related processes described in said patents solely for the research and development activities of the Durchaser when this product is used in Conjunction with a thermal cycler whose use IS Covered by the up front fee component Rights to the up front fee component must be obtained by the end user in order to have a complete license to use this Droduct in the PCR process These rights under the up front fee component may be purchased from Perkin EImer or obtained by purchasing an authorized thermal cycler No right to perform or offer commercial services of any kind using PCR including without limitation reporting the results of purchaser s activities for a fee or other commercial consideration is hereby granted by implication or estoppel Further information on purchasing licenses to practice the PCR Process may be obtained by contacting the Director of Licensing at The Perkin EImer Corporation 850 LincoIn Center Drive Foster City California 94404 or at Roche Molecular Systems Inc 1145 Atlantic Avenue
61. YRK AS24629 Code No 1 5mg p NHa2 C FAM 11 24 000 QTL LightSpeed MAPK QTL LightSpeed Assay Kit QTL LightSpeed Universal Assay Development Kit Assa Kit Y AnaSpec P 10 cAMP response element binding
62. dult colon cDNA library NubG x S cerevisiae CDNA library NubG x Saccharomyces cerevisiae CDNA library x NubG 100 ugx2 DsPO2211 455 000 NuDbG x Prey N NubG x NuDbG Prey C NubG lt DUALhybrid Yeast two hybrid system prey gt Mouse embryo cDNA Iibrary 100ugx2 0C DSPO2101 300 000 Human adult colon cDNA library 100ugx2 0C DSP0 210 300 000 HeLa cell line cDNA Iibrary 100ugx2 0C DSPO2103 300 000 7 Prey N Gal4 AD Gal4 AD prey Clontech Matchmaker V Iibrary HTX beta galactosidasSe assay Kit Y H
63. its x 3 BTQ 103 170 000 Blend Taq Plus 50Units x 1 BTQ 201T 6 000 Hot Start Version 50Units X 1 BTQ 201 1 000 Taqn PCH glBlend Taaq PCR PCR Pol I Taq Pol I DNA PCH Pol1I 3 5 Exonuclease Proofreading KOD Pfu o 5 Exo Exo PCR
64. or 488 HiLyte Fluor 555 HiLyte Fluor 647 HiLyte Fluor 680 HiLyte Fluor 750 Normalized Absorbance Normalized Fluorescence 500 600 Wavelength nm 700 800 500 600 700 Wavelength nm 6 HyLite Fluor Normalized abDsorption Fluorescence 1 488 acid 555 acid 3 647 acid 4 680 acid 5 750 acid PBS buffer HaN Protein o 1 SD S O C NH B 1 A A HiLyte Fluor 488 A aA A A Absorbance w FITC V We 0 20 40 60 80 100 120 G HyLite Fluor 488 FITC Irradiation Time min AnaTag AMCA X Protein Labeling Kit 347 447 Amino reactive dyeX5 Reaction buffer 0 5ml Spin ColumnX5 DMSO 1 ml Elution Duffer 10 ml Wash tuDeX5 Collect tube Xx5 AnaTag 5 FAM Protein Labeling Kit AnaTag 5 TAMRA Protein Labeling Kit AnaTag 5 ROX Protein Labeling Kit 5 5mg protein AS71000 5 39 000 AS71001 AS71002 AS71005 495 520 555 581 573
65. rosine hydroxylase 24 33 Ser31 MAP kinase MAP kinase ERK1 ERK2 p44 p42 MAPK ERK GSK 3 Presenilin 1 p38z p38 PKC Casein kinase I Casein kinase I Km 172M Vmax 6 Casein kinase I Casein kinase I Casein kinase 1I 150mM NaCI Km 60zM CDC 26 20 J Biochemistry 42 849 56 2003 J B C 274 9600 6 1999 J B C 278 5062 2003 J Cell Biochem 45 391 1991 Proc Natl Acad Sci U S A 89 2365 9 1992 Ann N Y Acad Sci 973 265 7 2002 Int J Impot Res 14 217 25 2002 Int J Impot Res 14 217 J B C 268 7646 1993 J B C 266 15277 1991
66. se 1 10 PLSRTLSVSS NH2 AS60517 1 29 000 AS60516 1 59 000 CaMK CaM KinaseIl Substrate 281 291 MHRQETVDCLK NHz AS29957 1 18 000 AS29955 1 53 000 CaMK AKT Substrate ARKRERTYSFGHHA AS29945 1 23 000 AS60505 1 59 000 AKI Crosstide GRPRTSSFAEG AS60513 1 29 000 AS60262 1 59 000 AKI CDK1 Substrate HATPPKKKRK AS60525 1 29 000 AS60524 1 59 000 CDR CDK5 Substrate Protein Kinase p34 cd2 Substrate MAP Kinase Substrate 3 EGF Receptor 661 681 GSK 3 Peptide Casein Kinase I Substrate Casein KinaseI Substrate 2 Tyrosine Kinase Peptide 1 PKTPKKAKKL 1mg ADAQHATPPKKKRKVEDPKDF AS60027 1 AS60286 1 18 000 AS29981 1 KVEKIGEGTYGVVYK 29 000 AS29935 1 52 000 CBDR 68 000 Tyrosine Kinase Peptide 1 amide KVEKIGEGTYGVVYK NH2 AS23988 22 000 EGFR Protein Tyrosine Kinase Substrate ADEYLIPQQ AS29936 1 30 000 AS29940 1 56 000 Tyrosine Kinase Peptide 3 RRLIEDAEYAARG AS22979 16 000 Tyrosine Kinase Peptide 3 amide RRLIEDAEYAARG NH2 AS29990 1 12 000 AS29989 1 58 000 p60c src Substrate I YIYGSFK AS60536 1 20 000 AS60529 1 59 000 Insulin Receptor 1142 1153 Insulin Receptor 1142 1153 amide TRDIYETDYYRK NH2 AS23998 30 000 CC TRDIYETDY
67. trate 1mg KRREILSRRPSYR AS23986 LRRASLG AS60527 25 000 RRRLSSLRA NHz2 AS60028 1 28 000 AS29984 1 47 000 AALVRQMSVAFFFK AS29927 1 29 000 AS6051 Labeled protein kinase substrates N TAMRA RA QTL LightSpeed Universal Assay Development Kit 1 1 AS29934 1 24 000 AS29926 1 59 000 53 000 60 000 PKC Substrate 5 1mg RRGRTGRGRRGIFR AS60545 1 29 000 AS60544 1 59 000 PKC6 Substrate 1mg RFAVRDMRQTVAVGVIKAVDKK AS60534 1 29 000 AS60562 1 59 000 Protein Kinase Cg Substrate 1mg ERMRPRKRQGSVRRRV AS60532 1 29 000 AS60531 1 59 000 MARCKS Protein 154 165 1mg KKRFSFKKSFKL AS29918 1 20 000 AS29916 1 56 000 Glycogen Synthase 1 8 PLSRTLSVAAKK NHz AS29915 1 20 000 AS29914 1 56 000 Neurogranin 28 43 PKG Substrate 1 PKG Substrate 2 BPDEtide Autocamtide 2 Autocamtide 3 Syntide 2 AAKIQASFRGHMARKK PLARTLSVAGLPGKK AS29991 1 AS29986 1 24 000 AS29992 1 24 000 AS29987 1 60 000 CaMK 59 000 CaMK Glycogen Syntha
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