TaqMan Small RNA Assay - Thermo Fisher Scientific
Contents
1. PCR 20 uL Optical Adhesive Film Optical Cap e Standard 20 uL AmpErase UNG PCR 40 cycles PR ek 50 C 95 C 95 C 60 C 2 min 10 min 15 sec 60 sec TUNG PCR PCR2. MultiScribe Reverse Transcriptase RNase Inhibitor RT RT 10 20 15 Lt YD 100 mM dNTPs with dTTP 0 15 uL MultiScribe Reverse Transcriptase 50 U uL 1 00 uL 10x Reverse Transcription Buffer 1 50 uL RNase Inhibitor 20 U uL 0 19 uL Nuclease Free Water 4 16 uL 7 00 uL T 7 pL 5x RT Primer 3 pL RNA 5 pL RT 15 LITRY EF RT RNA 5x RT Primer RNA RT Primer
3. a b Amplification Plot Baseline Threshold TaqMan Small RNA Assay Quick Reference Card For Research Use Only Not intended for any animal or human therapeutic or diagnostic use NOTICE TO PURCHASER PLEASE REFER TO THE TAQMAN SMALL RNA ASSAYS SUBMISSION GUIDELINES PROTOCOL FOR LIMITED LABEL LICENSE OR DISCLAIMER INFORMATION Trademarks of Life Technologies Corporation and its affiliated companies AB Design Applied Biosystems MultiScribe Silencer TaqMan AmpErase and AmpliTaq Gold are registered trademarks of Roche Molecular Systems Inc TRI Reagent is a registered trademark of Molecular Research Center Inc All other trademarks are the sole property of their respective owners 2011 Life Technologies Corporation Corporation All rights reserved Part Number 4412551 Rev C 01 2011 Y applied Headquarters SyS ms 5791 Van Allen Way Carlsbad CA 92008 USA a bio te Phone 760 603 7200 by Lk technologies www lifetechnologies com 44125519C Technical Resources and Support For the latest technical resources and support information for all locations please refer to our Web site at www appliedbiosystems com
4. 30 min 16 C 30 min 42 C 5 min 85 C oo 4 C PCR qPCR 1 qPCR qPCR PCR 4 TaqMan Small RNA Assay Quick Reference Card a b C 20 uL 3 uL 20 3 20 pL TaqMan Universal PCR Master Mix Il no UNG 10 00 uL 36 00 uL Nuclease Free Water 7 67 uL 27 61 uL TagMan Small RNA Assay 20x 1 00 pL 3 60 uL RT 1 33 uL 4 80 uL 20 00 pL 72 01 uL t TaqMan Universal PCR Master Mix II with UNG TaqMan Small RNA Assay RT
5. RNA Ambion Silencer Select siRNA c d 3 RT dn 4 Wi TaqMan Small RNA Assay Quick Reference Card c Ambion Silence Select siRNA 1 5 15 pL RT 0 2 mL 96 5x RT Primer 3 pL Sul 85C 5 60C 5 15 uuL RT RT RNA RT Primer RT 7 qL RNA RT 8uL 1 RNA 1 10 ng e
6. d RNA total RNA 1 4 5 15 pL RT RT total RNA 1 10 ng RT 7 uL total RNA 5 uL RT total RNA HAR 12 0 uL 0 2 mL 96 5x RT Primer 3 pl RT e e f 5 e Standard 15 pL
7. ey applied AS DIGSystBTm by Life technologies QUICK REFERENCE CARD TaqMan Small RNA Assay TaqMan MicroRNA Assay TaqMan siRNA Assay Custom TaqMan Small RNA Assay 7ggAan Small RNA Assay 4364031 SDS RT 1 total RNA a iat woh 2 RT 3 RT total RNA small RNA small RNA snoRNA total RNA TaqMan MicroRNA Reverse Transcription Kit 100mM dNTPs 10X Reverse Transcription Buffer
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