2X PCR Solution PrimeSTAR® HS (Premix)
Contents
1. v201403Da TaKaRa fe 07734319772. 74 1 DNA 2 10U 0 6 ug supercoiled pBR322 DNA 74 C 1 DNA ORG hot start PCR DNA PCR PrimeSTAR HS DNA Polymerase PCR PCR PCR 1 ADNA PCR 8 10 12 kb 2 DNA PCR 0 5 1 2 4 6 8kb PCR total 50 IPCR PrimeSTAR HS Premix 25 ul Primer 1 0 2 0 3 UM final conc Primer 2 0 2 0 3 uM final conc Template lt 200 ng up to 50 ul Template DNA 5ng 200ng lt 200ng E coli DNA 100 pg 100 ng cDNA 1ng 200ng ADNA 10p
3. dNTP Mixture 2 PCR PrimeSTAR HS DNA Polymerase 3 5 exonuclease DNA Taq DNA Polymerase PrimeSTAR HS DNA Polymerase 1 25 units 25 ul dNTP Mixture 2 X conc 0 4mM PrimeSTAR Buffer 2 Xx conc 2mM Mg2 PrimeSTAR HS DNA Polymerase R010A DNA 74 30 10 nmol 1U 100mM Tris HCI pH8 3 at 37 10mM KCI 2mM MgCl2 6mM NH4 2S04 0 1 Triton X 100 200 uM dATP dGTP dCTP 100 uM PH TTP 0 001 BSA 0 4mg ml DNA 1 10U 0 6 Ug O A Hind Ill
4. 2X PCR Solution PrimeSTAR HS Premix Code No R040A Size 500 lx5 for 100 PCR reactions Shipping at 20 C Store at 20 C Storage Excessive freezing and thawing may decrease enzyme activity Lot No Expiration Date For a detailed protocol refer to the product manual supplied with this product Description PrimeSTAR HS Premix is a 2X PCR enzyme mixture composed of Prime STAR HS DNA Polymerase a high fidelity DNA polymerase reaction buffer and dNTP mixture The premix formulation allows for quick preparation of reactions and reduces the risk of contamination This product is also useful for high throughput applications PrimeSTAR HS DNA Polymerase is a high fidelity enzyme that has a very strong 3 5 exonuclease activity It also has a higher amplification ef ficiency than standard Tag DNA Polymerase Composition PrimeSTAR HS DNA Polymerase 1 25 units 25 ul dNTP Mixture 2X conc ea 0 4mM PrimeSTAR Buffer 2X conc including 2 mM Mg Specification of PrimeSTAR HS DNA Polymerase Cat R010A Unit definition One unit is the amount of enzyme that will incorpo rate 10 nmol of dNTPs into acid insoluble products in 30 minutes at 74 C with activated salmon sperm DNA as the template primer Reaction mixture for unit definition 100mM Tris HCl pH8 3 at 37 C 10mM KCI 2mM MgCl 6mM NH4 2S04 0 1 Triton X 100 200 uM each dATP dGTP dCTP 100 uM BEHITTP 0 001 BSA 0 4
5. detailed cycling recommendations NOTICE TO PURCHASER LIMITED LICENSE P1 PCR Notice Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US 5 789 224 5 618 711 6 127 155 and claims outside the US corresponding to expired US Patent No 5 079 352 The purchase of this product includes a limited non transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser s own internal research No right under any other patent claim no right to perform any patented method and no right to perform commercial services of any kind including without limitation reporting the results of purchaser s activities for a fee or other commercial consideration is conveyed expressly by implication or by estoppel This product is for research use only Diagnostic uses under Roche patents require a separate license from Roche Further information on purchasing licenses may be obtained by contacting the Director of Licensing Applied Biosystems 850 Lincoln Centre Drive Foster City California 94404 USA M54 PrimeSTAR HS DNA Polymerase This product is covered by the claims of U S Patent No 7 704 713 and its foreign counterparts PrimeSTAR is a registered trademark of TAKARA BIO INC Note This product is for research use only It is not intended for use in therapeutic or diagnostic procedures for humans or animals Also do not
6. g 10ng DNA 10pg 1ng PCR 98 C 10sec 55 C 5sec 15sec 30cycles 72 1 min kb or 98 C 10 sec 68 C 1 min kb _ 30 cycles PCR
7. mg ml activated salmon sperm DNA Purity Nicking endonuclease and exonuclease activity were not detected after incubation of 0 6 u g of supercoiled pBR322 DNA or 0 6 ugof A Hind III digest with 10 units of this enzyme for 1 hour at 74C Applications High fidelity DNA amplification by hot start PCR PCR product A significant percentage of PCR products obtained using PrimeSTAR HS DNA polymerase will have blunt ends Thus the obtained PCR products can be directly cloned into blunt end vectors If necessary phosphorylate PCR products before cloning PCR test Good enzyme performance was confirmed by PCR amplification using both DNA amplified fragments 8 10 12 kb and human genomic DNA amplified fragment 0 5 1 2 4 6 8 kb as templates General reaction mixture for PCR total 50 ul PrimeSTAR HS Premix 25 ul Primer 1 0 2 0 3 UM final conc Primer 2 0 2 0 3 UM final conc Template lt 200 ng Sterile distilled water to 50 ul Recommended template amount Human genomic DNA 5 ng 200 ng lt 200 ng E coli genomic DNA 100 pg 100 ng cDNA library 1ng 200ng ADNA 10 pg 10ng plasmid DNA 10 pg 1 ng PCR conditions 98 10 sec 55 5sec or15 sec 30cycles 72 1 min kb or 98 C 10 sec 68 C 1 min kb EE Notice Because this enzyme has an extremely high priming efficiency a short annealing time is strongly recommended Please refer to the user manual supplied with this product for
8. use this product as food cosmetic or household item etc Takara products may not be resold or transferred modified for resale or transfer or used to manufacture commercial products without written approval from TAKARA BIO INC If you require licenses for other use please contact us by phone at 81 77 543 7247 or from our website at www takara bio com Your use of this product is also subject to compliance with any applicable licensing requirements described on the product web page It is your responsibility to review understand and adhere to any restrictions imposed by such statements All trademarks are the property of their respective owners Certain trademarks may not be registered in all jurisdictions v201403Da 2 X PCR Solution PrimeSTAR HS Premix Code No RO40A Size 500 pl x 5 for 100 PCR reactions Shipping at 20 C Store at 20 C Lot No PrimeSTAR HS Premix High Fidelity PCR PrimeSTAR HS DNA Polymerase
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