スイフトジーン® CPE産生ウエルシュ菌 「カイノス」
Contents
1. IC CPE mRNA IC CPE C CPE NASBA CPE 6 IC CPE a a DNA Membrane Li 1 TF 1 mRNA 2 3 1 4 TGP5000 2A3302. 70C 2 NASBA NASBA NASBA 30 uL 10 70C 3 20 30C 2 1 10 200 1000 plL 2 41 90C 3 4 3 A 1 10 1 mL 1 890 G 1 100 pL 2
3. 7 2013 7 2 2012 9 1 1 1 1x10 cfu g 2 Aeromonas hydrophila Staphylococcus aureus Clostridium perfringens cpe Vibrio parahaemolyticus 1 1 2
4. 1 2 3 4 RNA RNA 1 1 2 NASBA NASBA Reaction Buffer dNTPs NTPs 3 CPE 4 NASBA NASBA Enzyme AMV RT RNase H T7 RNA polymerase 5 NASBA 6 2 1 2 3 1 NASBA Nucleic Acid Seguence Based Amplification
5. CPE mRNA A RNA B CPE CPE mRNA 41C 3 AMV RT H RNase H RNA T7 RNA polymerase 2 DNA 1 RNA RNA _ 5 T7 4 2 wmr P1 mRNA DNA RNase H P1 AMV RT C NASBA DNA CPE
6. 3 2 1 2 2 3 NASBA 20C 70C 4 5 3 1 2
7. IC IC r r r CPE 1 CPE 2 CPE 3 2 1 20 2 15 3 4 CPE IC CPE CPE 5 IC CPE 6
8. 3 4 2 10C 12 TGP5000 2A330 2013 7 2 2012 9 1 NASBA NASBA NASBA GP 5200 GP 5100 1 J Compton Nature 350 91 92 1991 2 36 19 24 2007 113 0033 2 38 18 B03 3816 4480 FAX03 3816 6544 Ks 113 0033 2 38 18 03 3816 4485
9. 1 4 41C NASBA 2 5 uL 5 41C 30 4 NASBA 5 NASBA 5 C 5 NASBA 20C C 1 20 30C 6 2 NASBA
10. 3 90 uL 3 7 8 4 15 tt l 1 3 2 3 15 TGP5000 2A330 1 1 CPE r CPE 2 r IC CPE 3 r r
11. 100 pL 400 10 3 90C 5 5 1 890 G 10 4 NASBA 1 15 NASBA 2013 7 2 2012 9 1 B NASBA 1 NASBA 5 2 A 4 2 5 10 2 RNA 3 41C 5 3 NASBA
12. 1 1 10 259 225mL 1 109 2 10 2 1 RNase 109 2 20 30C 1 1 NASBA NASBA 55 uL
13. TGP5000 2A330 2013 7 2 2012 9 1 CPE Clostridium perfringens Clostridium perfringens enterotoxin CPE CPE 10 CPE CPE
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