「マウスノロウイルスの状況と研究」 「遺伝子改変
Contents
1.2.
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4. 1
5. 2009 Information Technology
6. 42
7. 174 8505 3 6 10 TEL 03 3968 1192 FAX 03 3968 4863 URL http www oyc bio jp E mail fbi oyc co jp KG LABIO 21 OCT 2009 13
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9. Pasteur Pasteur 1824
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11. SCAW 9 SCAW 1988 No 7 1 20 9 29 9 1987 SCAW SVG
12. 2005 JaCVAM JaCVAM JaCVAM 710 2006 JaCVAM 1ICCVAMT2
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14. e 272cs 7222277 8 Norwalkvirus CO Murine norovirus MNV 2003 Karst 9 Cal
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17. 10 36 3 LABIO 21 OCT 2009 f 21 7
18. complex traits RI c RI RI c c RI Strain Distributon Pattern SDP R 956 i c
19. Roger W Barrette 1 3 3 1989 1996 3 1 1989 2
20. HNV MNV RAW 264 7 MNV MNV S7 ELISA MNV MNV HNV MNV 1 MNV 3 1 MNV ICR RNA 0 2 4 8 14 28 42 56 0 0 0 0 nd 0 0
21. GHANNEL SCIENCE CO tTO 5 Towner JS et al Isolation of genetically diverse Marburg viruses from Egyptian fruit bats PLoS Pathog e1000536 2009 6 Swanepoel R et al Studies of reservoir hosts for Marburg virus Emerg Infect Dis 13 12 1847 51 2007 38 LABIO 21 OCT 2009 http www channelsclence CO jD T1670052 4 29 10 TEL03 3331 7252 FAX03 3331 7347 gt 2008 6 13
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25. KAZE KAZI LABIO21 KAZE KAZTI STAFF HARUO NIIZEKI AKIO YAMADA MASAKI ARAMAKI SEINOSUKE 0HSHIMA KIMIO KAWANO EIICHI KAWAMOTO MINORU KITOH MASAHIKO KUSANAGI TAKATOSHI KUHARA YASUHIRO SAKURAI AKIHIRO SHIHASHI MICHIO MAE TAKEHIRO SEKI NARIAKI KUDO TTI LABIO 21 No 38 1 10 1 101 0051
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29. 1 Barrette RW et al Discovery of swine as a host for the Reston SClence 325 5937 204 6 2009 ebolavirus 2 Pourrut X et al Spatial and temporal patterns of Zaire ebolavirus antbody prevalence in the possible reservoir bat species J Infect Dis 196 Suppl 2 S176 83 2007 3 Biek R et al Recent common ancestry of Ebola Zaire virus found in a bat reservolr PLoS Pathog 2 10 e90 2006 4 Leroy EM et al Fruit bats as reservoirs of Ebola virus Nature 438 7068 575 6 2005 8
30. 1989 LABIO 21 OCT 2009 f 37 6 2 6
31. 1972 SPF NZW 46 LABIO 21 OCT 2009 WHHL SPF NZW 1986 1991 MA WHHL
32. 12 6 2 3 10 1 3 3 LABIO 21 OCT 2009 f 23 EED ADME TOX
33. 3 2009 180 000 70 2011 2017 Alternative 11t EPAA European Partnership on AlternatiVe Approaches to Animal Testing 3Rs 300
34. MNV 1 1 Hsu MNV ICR 10 MNV 1 MNV 2 MNV 3 MNV 4 0 56 LABIO 21 OCT 2009 f 7 RT PCR RNA 1 MNV 1 2 RNA MNV 2 MNV 3 MNV 4 ICR RNA MNV
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37. 4 TICATM International Cooperation on Alternative Test Methods 19 NICEATM National Toxicology Program Interagency Center for the Evaluation of Alternative Toxicological Methods ICCVAM ECVAM ESAC ECVAM Scientific Advisory Committee Health Canada JaCVAM 4 OECD JaCVAM 2 ICATM
38. 1950 11 28 75 1 Animal Care Panel ACP Brewer ACP ACP Proceedings of the Animal Care Panel Laboratory Animal Care Laboratory Animal Science ACP 1967 Manual for Laboratory Animal Technicians ACP Guide for Laboratory Animal Facilities and Care
39. IFN RAG STAT1 IEN g y 3 Karst IFN RDA 7 382bp HNV
40. 1 MNV 1 Karst MNV 1 MNV ITFN o CsCl 1 36 0 04 g cm3 HNV 28 35 nm RAG STAT1 MNV 1 MNV 1 IFN gu STAT1 RAG STAT1 3 1 MNV 1
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42. REACH Registration Evaluation Authorisation and Restriction of CHemicals 2003 7 2009 3 EU 2 EU COLIPA ECVAM European Center for the Validation of Methods 2009 REACH EU
43. MNV 2003 MNV 1 MNV 2 MNV 3 MNV 4 MNV 5 MNV 6 MNV MNV HNV 7 MNV HNV
44. 1 6 44 43 A GFP GFP 3 40 6 GFP P lt 0 01 1 TT re I i 3 GFP a b c Reduction Refinement 16 LABIO 21 OCT 2009
45. Galen AD 130 200 0 16 Andreas Vesalius 1514 1564 1628 William Harvey 1800 Francois Magendie 1783 1855 Claude Bernard 1813 1878 Louis Pasteur 1822 1895 Bernard 1865
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48. MNV CW3 3 129 F4 80 CD11c B220 F4 80 CD11c B220 B CD11b CD4 CD8 MHV MHV MNV Ward MNV 8 Ragl IFN y
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50. Goto K et al 2009 First trial in the developmental phase of the Performance Evaluation Program based on the ICLAS Animal Quality Network Program Self assessment of microbiological monitoring methods using test samples supplies by ICLAS Exp Anim 58 47 52 kagiyama Vetmed hokudai elD 3 CIOMS 1985 International Guiding Principles for Biomedical Research Involving Animals CIOMS CIOMS TICLAS TICLAS 2008
51. 4 LABIO 21 OCT 2009 OIE CC
52. 8I 2007 ICLAS 59 LABIO 21 OCT 2009 f 11 3 RT PCR 3 nm Wm gt CcC _ MNV Accession Number MNV 4 polyprotein DQ223043 MNV 4 polyprotein DQ223043 MNV 4 polyprotein DQ223043 MNV 4 polyprotein DQ223043 MNV 4 polyprotein DQ223043 MNV GV CR15 2005 USA EU004681 MNV GV CR3 2005 USA EU004673 MNV GV CR13 2005 USA EU004680 MNV strain Berlin 04 06 DE DQ911368 MNV strain Berlin 04 06 DE DQ911368
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58. 179 0074 6 10 40 TEL 03 3990 3303 FAX 03 3998 2243 42 W LABIO 21 OCT 2009 Information on Overseas Technology MFI HBUUJOJUI uol Multiplex Fluorescent Immunoassay MFI well MFT MFI MEFTI
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61. NSMR Anton Carlson NSMR 1952 4 15 Robert Gesell Gesell NSMR NSMR Gesell NSMR Carlson Gesell 2 Gesell 1954 Gesell Gesell Christine Stevens Animal W
62. Reduction Refinement iPS ES Replacement 1 Sasaki E Suemnizu H Shimada A et al Generation of transgenic non human primates with germline transmission Nature 459 523 7 2009 2 Wolfgang M Hisele SG Browne M A et al Rhesus monkey placental transgene expression after lentiviral gene transfer into preumplantaton embryos Proc Natl Acad Sci U SA 98 10728 32 2001 3 Chan a W Chong K Y Martinovich C et al Transgenic monkeys produced by retroviral gene transfer into mature oocytesS SClen
63. P450 P450 http bio nosan cO jD 9 TEL 045 224 3740 e mail bio nosan co jp 0 4 ANIMAL Nosan Beagle Narc Catus PROTEOME
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72. CRE WNW 1 J G Fox L C Anderson F M Loew F W Quimby Eds Laboratory Animal Medicine 2nd Ed Academic Press 2002 2 W M S Russell and R L Burch The principles of humane exDerimental technique Methuen London 1959 3 Anonymous Consensus recommendations on effective institutional animal care and use commnittees Laboratory Animal Sclence SDeclal Issue 11 13 1987 4 No 7 20 14 17 1988 1 1942 2 1975 32 LABIO 21 OCT 2009 5 LABIO 21 29 35 38 2007 6 2 232 247 1990 7 4 154 167 1992 8 2004 9 162 127 133 2008 10
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75. aboratory animal science Aristotle 384 322 B C Erasistratus 304 250 B C
76. FELASA A B C D 4 ICLAS 2 Americas Region Regional Committee 22 W LABIO 21 OCT 2009 ICLAS journal Asian Region ICLAS FESSACAL ACCMAL Biomodels applied in development and technology innovation 7 Plenary Session 6 Symposium Lecture
77. RN B5 86 ISBN 4 900659 72 X 2006 11 28 37 Suppl 1 July 2003 refinementO 42giame omime tk 1 260 mo hf reduction WR 4 5 Laboratory Animals37 164 OOO3 4 27 37 TEL O3 5925 2840FAX O3 5925 2913 E mail book adthree com URL http www adthree com LABIO 21 OCT 2009 f 3 21 21
78. ECVAM JaCVAM 4 OECD 19 OECD 1 18 LABIO 21
79. well MFI 5 6 m 1 2 Fig 1 100 100 1 2 000 2 500 well 2
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81. ICLAS Lactic dehydrogenase elevationg virus Mouse adenovirus Pneumonia virus of mice 95 9 95 1 95 1 95 3 95 1 94 2 95 7 94 4 95 7 95 7 95 1 real time PCR C57BL 6 BALB c
82. ICLAS ICLAS ICLAS Demers Canadian Council for Animal Care 12 ICLAS National Member Scientific Member Union Member Associate Member 2006 6 36 52 37 125 2007 2011 Initiative 1 2 Animal Quality Network Program 3 CIOMS Guiding Principles 4 OIE 4 1 Principles 5
83. Institute Resources IAR IAR 1953 IAR 1956 Institute of Laboratory Animal Resources ILAR 1990 Institute of Laboratory Animal Research ILAR IAR Orson Eaton Berton F Hi 10 1955 1965 Little Eaton Hil 1965 Robert H Yager of Animal LABIO 21 OCT 2009 29 LAM 1 Yager 1952 ILAR ILAR 1967 International Councll on Laboratory Animal Science ICLAS 1979
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100. 1 3 21 12 8 1 2 2 LABIO 21 OCT 2009 49 3 22 2009 11 13 15 27 2009 11 14 39 2009 12 2 4 4 60 National Meeting AALAS 2009 11 8 12 Denver Colorado http www nationalmeeting aalas org 2 2009 11 17 13 30 38 2009 11 26 27
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104. 2008 http www enV go jp nature dobutsu algo law_series nt_h180428 88 html gt ICLAS 2009 6 1 2 ICLAS 3 6 2 5 3 4 ACCMAL Associations of Central America Caribbean and Mexico Laboratory Animal Science FESSACAL Federation of South American Societies and Associations of Laboratory Animal Specialists 1 1 lglesia Matriz ICLAS Co chair of ICLAS Asian Region 5 30 6 6 4
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108. 1986 1986 1968 Canadian Council on Animal Care CCAC CCAC Harry Rowsel CCAC Guide to the Care and Use of Experimental Animals CCAC 1980 CCAC
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111. JICLAS URL l Fox JG Dewhirst FE Tully JG 4 Goto K Ohashi H Takakura A 9 Karst S M Wobus C E Lay M Paster BI Yan L Taylor NS Collins M Jr Gorelick PL Ward JM Helicobacter hepaticus sp nov a microaerophilic bacterium isolated from livers and intestinal mucosal scrapings from mice J Clin Microbiol 1994 May 32 5 1238 45 Ganaway J R Spencer 1 H Moore T D and Allen A M Isolation propagation and characterization of newly recognized pathogen cilia associated respiratory bacillus of rats an etiological agent of chronic respiratory disease 1985 Infection and Immunity 47 472 479 Goto K Hayashimoto N Yasuda M Ishida T Kameda S Takakura A and Itoh T Molecular detection of murine norovirus from experimentally and spontaneously infected mice 2009 Exp Anim 58 135 140 and Itoh T Current status of Helicobacter contamination of laboratory mice rats gerbils and house musk shrews in Jap
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119. PCR PCR 1 RNA polymerase gene MNV Accession no EU004676 95 7 MNV A5HIS9 99 3 Capsid protein gene MNV EU004671 98 4 MNV A5HITO 100 MNV MNV DDB DNA Data Bank of Japan http www ddbj nig ac jp accession number AB469416 CUW72 PCR 6 C B 17 Prkdcsed scid 6 JcLICR ICR 3 MNV
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121. 100 2 METI ELISA ELISA MFI 1 100 well 0 2 7 MEFI ELISA VP2 NS1 MFI ELISA
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125. MNV GenBank MNV NC008311 RNA polymerase gene 1st PCR FE 5 GAC ATC ATG GTG CGC CT 3 R 5 CTC ATT CAC AAA GAC TGC TG 3 2nd PCR N 5ICOY TIO TAT GGT GAT GAY GA R 5 TCT CAG CAT CCA TTG TTC CA 3 466bp 3 MNV PCR MNV PCR ICLAS PCR Hsu 6 Hsu Capsid protemm 187bp
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128. SS RNN http jaeat kanto adthree com 4 a Me http jaeat org 48 LABIO 21 OCT 2009 21 9 8 60 1 15 15 16 00
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131. 17 110 million REACH 100 125 INVITTOX 2009 40 50 in silico 20 7 2011 OECD ECVAM LABIO 21 OCT 2009 f 17 ICCVAM Interagency Coordinating Committee on the Validation of Alternative Methods 8 3 3Rs
132. TestDiet PMI HP http www labdiet com LabDiet BT nSsLC 053 486 3155 053 486 3157 T431 1103 337 1 8 TEU2 0 sIclSo TEL 053 486 3178 FAX 053 486 3156 http www jslc co jp Small players ina better future For the future New development 7 REGISTERED ym JAB CC No 2827 ISO 9001 QMS http www CLEA Japan com Jl
133. 60 Donald Bailey 2 BALB cBy C57BL 6By RI 7 3 8 11 5 A A BB F1 F2 A A A B B BB F 20 A B RI RI
134. scid ICR 3 2 1009 6 3 3 ICR 100 3 3 14 21 56 3 3 3 3 3 3 4 4 4 4 4 4 PCR 8 NOD scid MNV 5 9 NOD scid MNV 18
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142. 2 16 15 18 00 2 1 9 6 2 2 1 6 2
143. MNV strain Berlin 04 06 DE DQ911368 245 MNV PCR MNV 33 245 13 1 15 59 25 4 MNV 15 11 MNV 3 MNV 4 MNV GV MNV Berlin 04 06 DE 94 2 MNV MNV SPF 15 MNV strain 12 W LABIO 21 OCT 2009 2003 MNV scid
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