マニュアル SWIFT II
Contents
1. GE imagination at work GE Healthcare Index2. 1nm 2 Point Point Maths 360nm 14 eben atd Save pa 00140 0 EE BiE em we _ Corst
3. GE imagination at work GE Healthcare Modify Run Method Run Default
4. 7 6 Quantification Post Run Options Slope EE 3 a ry ht Et SWIFT I Quantification greendye QAD File Methods Edit View Run Slope Window Help Plmm Linear Regressinn Linear Interpolation Manual Result v Force Zero Extrapolate curye Figure 169 GE Imagination at work GE Healthcare OwIlr
5. 4 2 3 Display Page GE imagination at work GE Healthcare Farameters histails Display Run ntinns hs WT Rangg Scah hode inimum Im hs ax mam nnn Fisure 105 Minimum Abs 0 0 3 0 Maximum Abs 0 0 3 0 Abs Tmode 4 2 4 Run Options Page Parameters hietails hisnlay Fun Uptions Post run ME Function I Save Eidia raph Derivative Gawe Sbreadsheet Overlay Graphs PFg Fimd
6. 260 nm 280 nm Christian Warberg Protein conc mg ml 1 55 Abs 280 0 0 76 Abs 260 0 1 55 0 76 User Method 9 Define Method Add
7. 0 GE Imagination at work GE Healthcare Farattuetefg ahhh Ft lear Lt nRY Faste Figure 2 1 4 4 Status Bar
8. GE Imagination at work GE Healthcare Time Drive Assay bort Display Scale ll amed Runhing Assay LE Duration 8 37 Wawelenegth 5 Next Reading HH Mssay bsorbarnce IEH Figure 149 Abort 6 0 CA0 6 5 1 Single Sample Mode 1 Wavelength lag time duration period
9. LP Test LP Test1 LP Test2 LP Tesh3 Figure 72 new load run focus GE Imagination at work GE Heqlthcare Parameters a View A View B errrative Frmt Tnnlhar Frimt Preview tatms Bar tnmse Palette Figure 73 3 5 3 Data View Tiata LE Test 1 Wardcup hh rm ml nie View Wavescan X
10. Methods 2 4 5 1 Standard Mode 1 4 5 2 Repeat Mode CU 1
11. G GE imagination at work GE Healthcare gt 50 5 lomse Falette Figure 79 Delivative 3 5 6 Results View scan oF hanlrmum Fker 2 ei Find ts a IFektupe _ 1Fosn top 1 _ Fisgure 80 GE imagination at work GE Healthcare New Clear TaTTIEtETS Peak Find Cascade Et J i Td Feak Tea Tile Arranege Icons hiouse Falette Frint Preview Fnmt Setop Exit Figure 36 Typical R
12. Heating and Cooling 10 4 1 Run Parameters Run Run Parameters Filename 1 2 Reference Options Setup Control Options OK Parameters
13. 4 5 3 Use with Sipper 1 4 6 Wavescan Post Run Options GE imagination at work GE Heqlthcare Post Run Smoothing Mathe
14. 9 6 Fraction Analysis Post Run Options Fraction Analysis Post Run 7 GE imagination at work GE Healthcare 10 Tm MELTING POINT Tm 10 1 Tm 260nm Tm Tm 50 DNA DNA Tm
15. Method GE Imagination at work GE Healthcare Method Parameters Parameters Parameters Details Display Run Options
16. LOG LOG Windows GE imagination at work GE Healthcare gt gt gt gt C Program Files SWIFT II
17. Open Find Close _ Save Save As Method File Data File Standard File Spreadsheet File Export Set up Print Index Graph Spreadsheet
18. GE imagination at work GE Heqlthcare hiethod A hiethod B Tjefine Method Diefault Settings Figure 6 Irpical Application Main Menm Figure 71 000 3 5 2 Group Tree View
19. GE imagination at work GE Heqlthcare WIFT Imstrument Gontral File ew Enmmand LataLng Help wl Frint GT F Print Prewiem TT Frint Setup 000 ahce hiode PETRIE La CCone Call 1 Factnr 1 nnd Mext Ci Isetun nlicatin Figure 13 Setup File gt Setup menu tab page setun Default E34 chemes Raggistratinn niITILIhIEatI ITS breadsheet LarIEUaEBE LF hata LnggIing Default WE Lig letg _ my i AM2 Figure 14 Schimes GE Imagination at work GE Healthcare setun Default Schemes Pegistration Cammunicatians breadsheet Lahneglaee FjLF Data Loegegine Name HHress jnstrurmmnt Serial umher B
20. mg Biachrom Lt ddess 2 Cambridge Scence Park Mlilton Hnad Cambridge PB DFJ Ennlard stnarment Seral Mumbe II Figure 65 3 4 7 Communications Ultrospec 2000 3000 4000 Ewtermal Feltier None nstrurment Com 1 Com3 Fnm 3 Com4 Com 1 Com 2 Fnmg Lnm Ltrospsc 3HH pro Fhgck Fnr Estermal Peltie Higure 66 3 4 8 Spreadsheet Microsoft Excel Tab GE imagination at work GE Healthcare Direct ln Ecel Fieset Totertfile Commsa t Tab Figure 67 3 4 9 Default Windows WS
21. Lineweaver Burke Hanes Woolf Badie Hofstee Vmax Km Kcat Hill View Michaelis Menten Plot GE imagination at work GE Heqlthcare 2 Reaction Kinetics Parameters 5 2 1 New Parameters Details Display Run Options 4 New 4 Parameters lock
22. Clear Copy Michaelis Menten View Data Points CopyGraph Label Add Edit Move 6 Reaction Kinetics Display Options GE imagination at work GE Heqlthcare 6 1 View View View
23. Define Region 3 3 4 View File hethods Edit Wiew Fn Fost Run Window Help Graph N Dreadsheet er ay Lishlay Lnscalg w Innlhar wtatug Bar w ausg Fallstg Higure 53 View Parameters
24. Peak Hesults Ix sear lhanlmium hiker 2 2 Pe _ absorbance 1a Na FeakTye Fosion Heht LAs stat 1End br IE HH LH7 1 4 dF 4 Figure 95 Peak Area Results Area 3 6 6 Derivative 1 2 4 GE imagination at work GE Healthcare grlwatly Dn rgnglDaida Oset Cowouim First Dereate ly EE wide Second Deriwative Fhedium Fourth Liereatiee Harrow Enharnc gd Fi _ sat Figure 96
25. Parameter lock 7 2 1 Standards Parameters and Details Page GE imagination at work GE Healthcare Parameters Standards Run Options Trtlg perator Foients Filename assay po Wawelenegth an Temperature gthnd actnr StanHard urwg puhstratg Concehtration Figure 152 Title _ Operator Comments Filename Wavelength Temperature Method Substrate Concentration Standard Curve Factor
26. IFT I Imstrument Gontraol File le nmmand Data Lng Help Cannect MaglEhgt 160 CCESS TW ma mm bs Mnde Rferahd Fa all 1 Reterehce heck MAccessory Next Le Configure Ruh Wawelernegth functions Coml Figure 31 Command NM Command GE imagination at work GE Healthcare Wavelength Enter Wavreleneth Figure 32 Wavelength 190 0 1100 0nm
27. Parameters Parameters igtalls hiisnlay Ruh Options hssay Farameters gthnd Serial ode ho ot ssaws Farallel Mode Synchrnnisgd Time Lnits Lag lim Luratinn Mti mWaye yeE ngth 340 0 Fgrind Rieterence Get Rieterence Temnerature nn Filename hata Assay Figure 39 Save GE imagination at work GE Heqlthcare 2 E34 kinetics Default RK hssay RE MetnodwRKD Figure 40 To Lock
28. Parameters Detals Fost Hun Display Hun rtiars Tita Laralai Comment FT Fename Assay2 12 11 5 eh Figure 196 Title Operator Comments Assay Parameters Assay No Details Filename Parameters 1 10 2 3 Post Run Display Page GE imagination at work GE Healthcare Paramalars hialals PostHunLishIaW Rur piors Eo Hi Mone ras Soid Ticks Innm Fesst Figure 197 Maximum Abs 0 0 3 0
29. Point GE imagination qt work GE Healthcare Peak Find 1 Peak Find Results Peak Area Dropped Baseline Sloping Baseline Derivative Slope Reaction Kinetics
30. Run Modify Run Method Run Default standard 1 6 Quantification Multi Wavelensth Fraction Analysis 1 5 Method Data Parameters 3
31. 0 001 10 000 1 1 1 Units Assay No Parameters Assay No Details Hilename Parameters 1 Update all assays Parameters Assay No Details 2 1 3 Display Page GE Imagination at work GE Healthcare Framaters hatailr Desplan Fi ptinns Imltial ss Duranhian IF Enable IL Increrrental we Fr Enabke 10 hfault Te rils rnrre bsobancs hm iimum boon hakimum Bm Figure 11
32. 0 0 Bdit gt Meree 3 View Display GE Imagination at work GE Healthcare Edit Window Clear Peak Find Cascade opy Graph Peak Area Tile ncale Dierivative Arranege Icons Om Trace View A View Figure 85 3 5 9 Michaelis Menten View
33. 10 4 2 Run Time View GE imagination at work GE Healthcare Run Heating and Cooling cooling Run Abort Hide Show Display
34. 7 4 Trace SWIFT 8 Michaelis Menten View gt Michaelis Menten Plot Michaelis Menten Michaelis Menten Bdit gt Data Points Add Set Parameters gt Details
35. GE imagination qt work GE Healthcare Farameters Farameters hietails Display Run Options hssay Farameters Tirme Lnits hhimm ss Lag Time Hm Luratinn 1 HH Fgrind i Wawelernegths Ri 1 nf 3 awe Filename Datal hssay Lock Temperature tn Fieterence eone lse Reterence Blark se Sample nt Start se Sample tt Lag Time Figure 150 Parameters Parameters igtails iisnlay Run Options ssay Parameters Time LUmits hh mim ss Lag limie nn Luratinn 1 HI Fgrind mm aveleneth ts H5n E 4 ot R Save Filename Datal ssay Lnck Temperature Fr Raferehcg one ILse Referencs ank Lse Sample nt Start Lse Sample ht Lag Time En Figure 151 2 6 5 2 Use with Sipper 6 6 Time Drive Post Run Options Post Run Mathematics P
36. OK S V Michaelis Menten FETE Tle ume wet nH gl IMETHYLFK4 Add Point D320 1 500 1 280 5 240 7 5n MM 4 BI 11 500 Bm 15n BanoveFonl 14 500 1 5 30 B0U 2n nn Figure 129 GE imagination at work GE Healthcare Figure 130 8 1 Michaelis Menten View
37. Results Graph te Graph Results GE imagination at work GE Healthcare ITHlE Title ho bs ample 11 aIhle 2 18 lB 1 1II a pl 4 4 I a mple 5 In4H ample 5 HF Figure 189 Fractions Fraction analysis demonst Figure 190 9 5 Fraction Analysis Run Method Data Samples Parameters
38. 3 4 1 Print Option GE Imagination at work GE Heqlthcare setun hDefaul Lina Styles LF uHit ra Lirectnrles Lisfault WWindnms Che mes Fiegistration Cnmunicatinns breadsheet Larneglaee Frint Dptons Ba Graph Spreadshest Feset Lafaults LI Header Ltd LF rennrt Parameters H ranh Hiesults inITT TB Rie mowe Figure 60 Print Option Print Preview Wavescan Reaction Kinetics Time Drive Tm Index Window Reacton Kineiics Michalis Menten Quantification Multi Wavelen
39. Microsoft Bxcel GE imagination at work GE Healthcare setun Default Sache hes Reegistration Gammuhnications Spreadsheet ELI EE iLF Lata Loegeine Hirect tn Excel io text file haCITE ID Conlon nmmma Space Th Dther OE Fisure 19 Text file setun Default rhg ITIES Registratinhn niTuhICatin ns Spreadsheet Lariguagg GLP Data Loegegineg Figure 20 Language A GE Imagination at work GE Healthcare Setup Default Che mes Registratinn Cnmmunicatinnhs Spreadsheet Laneguage LP Lata Logeging Frint nn completion Frint with data loe Ee Figure 21 GLP G
40. Substrate Concentration Repeat reference ON 1 Samples 2 3 Substrate Concentration 7 3 Quantification Display Options GE imagination at work GE Healthcare Quantification V
41. Parameter Quantification ID Multi Wavelength Fraction Analysis
42. 1 All Users Winnt Profiles installer Start Menu Programs Winnt Profiles All Users Start Menu Programs 2 GE imagination at work GE Healthcare Winn 0Profiles All Users Programs Properties gt Security gt Permisslon gt Add gt Highlight All User gt Add gt OK SWIFT II MBTHOD PROGRAM FILES Instument Instrument Wavescan Wavescan Kinetics Kinetics TDrive Tdrive Quant Quantity Multi Muli Eraction Fraction Culture Culture Tm Tm Tm 1 3 Helpful Hints
43. Repeat reference ON GE imagination at work GE Healthcare 1 Samples 2 3 8 3 File Eile New Method Default M
44. Grid On Off GE imagination at work GE Healthcare isplan hnns rd hoe Lags Pe ingz ld Ticks Hasat lm Figure 199 Display Options Calculator Tm Tm Calculator Tm WM hnlyul 5 0 R TAAAACGACGGCCAGT_ Es Lnncghlralinn Im lu mm lt alt nd Huffer nm Imnl 1 Llgar Cielete CO Bhs DNA lnse Figure 200 Tm Tm Tm RNA ssDNA T U 16 64mer gt g ml mol 1 gt
45. pmol xz 1 Scale Auto Full Region Zoom Zoom Auto Full Region GE imagination at work GE Healthcare Unscale Zoom Scale Toolbar Status Bar Mouse Palette 10 7 Tm Post Run Options Tm 10 7 1Tm Calculation Tm Auto Calculaiion Tm Display Results Manual
46. Tm Display Results ClearResults Display Results Auto Manual Tm STFT I Tm TYPIGALTMD File Methods Edit View Run Fost Run Window Help MM Graph Typical Melting Curve lt Abs Typical Melting Curve i Typical Mel Typical M 78 1 Ee 97 7 s HI91 367 Tm 867 Temp CO Tm Qffset 867 GE Imagination at work GE Heqlthcare Fieure 201 10 7 2 Derivative OK Derivative
47. Multi Component Analysis JCAMP JCAMP JCAMP ASCII GE imagination at work GE Healthcare Common Menu Descriptions 3 3 Post Run Menu Post Run Menu Filg hethodg Edt iew Pun mi Windo Help oath
48. log LOG Parameters Lock Unlock Audit Trail Lock Unlock LOG GE imagination at work GE Healthcare 2 LO Instrument Control Panel Instrument Control Panel
49. Method File Data File Standard File Spreadsheet File Bxport Set up Print Index Graph Spreadsheet Michaelis Menten File gt Set up gt Print Options Print Preview PC Set up gt Print Options Print Set up Common Print Dialogue ag Exit 5 3 Method Method Parameters Default
50. Peak Width Peak Hesults 1 scan of hanlmum Fiker 2 Feak Find Ebsorbance No FeskTwpe Fosiion Heigm 1 Figure 93 Peak Find Results GE imagination at work GE Healthcare FilezPrint 3 6 S Peak Area Peak Area Dropped Baseline Sloping Basehne Ceanch Area EraElH Hasalina ensitiity hg Dropped Sloping Heset Fisgure 94 Peak Area Define Area Dropped Baseline Sloping Base Line
51. GE imagination at work GE Healthcare Simple path for runnming an experiment a Run 1 Applcation Load Method Select Method menn option i Choose Method from list Create Mew Method Select File New Edit Method Edit Commmon Parameters Edit Sample Farameters Customise Method save Method Select Data to be roan Ouantific ation and Multi Wavelength nl select Samples Select sample to toggle OnOF Figure 37 SWIFT II 3 3 Common Menu Descriptions 3 3 1 File GE imagination at work GE Healthcare EE ICP BSCah RK Tirme Lriwg EH liantiti WEFT I Warescan File hethods Wiem Fun Help em WEJU ni tH etup Frint Setup Prngram Filgs SWTF IIWawgscar Liafaul wsm CE FRIOGR FILESTSWTFT WESO HILLHM FILTER IIB 1 B 1 d4TWShD PRUGR FILESWSWTFT WayESGOMWGWMHFT1 RGHF WS PROGR MM FILESTSWITFT Wa EGGOMWIMMPGRTED sD Exit Fisure 38 File INew Method
52. 3 3 1 SWIFT I 3 2 3 3 Set up Hile gt Set up 3 4 3 5 Post Run 3 6 3 2 Running in General SWIFT II
53. Fnr Help press F1 FUM Figure 3 1 4 5 Toolbar and Mouse Palette Hile New Open Save Setup Edit Cut Copy Paste View Parameters Graph Options Run All Modes Print Help About 9 W Figure 4 Trparal Application Tnnlhar Figure 4 Zoom Add 1abel Move Trace
54. Abs Compound Impurity A A hsorbance UiffBrarrcg A 1 AA2 Figure 177 Absorbance Ratio Measurement GE imagination at work GE Healthcare 2 max be lr neerlaid spectra of canUTd ay rl iTTmfmirtty nl Uy EET IF TRITE ctrl mo imPmlrlr mesent dH SFechrumm nf ompound ml mpianmiy hs Figure 178 1 2
55. View Michaelis Menten Lineweaver Burke Hanes Woolf Eadie Hofstee 4 Overlaid GE imagination at work GE Healthcare splay tr Eadie Hofstee Display Intercepts Lineweaver Burke Toolbar Hanes Woolf View A Stahuns Har Wiemw lomse Palette Print Preview Print Setup Figure 86 Michaelis Ment
56. New GE imagination at work GE Healthcare SWIFT I Culture SIGK I Filg Methods Edit Wiew Run em Lineh CT Clnse a CTT wE E Export etub Frint Ctr F Frint Frewiewm Frint Setup Detault cum gal TIUGEDGHD Exit Figure 207 New Open Farameters Parameters etai Fnst Run Displaw Split Lietais Ile F f F Trmneraturg ssay FararmatErs Wawalergth 4nn Filename Diata Eigk Induced Rieterence GTiE Before Each Rn We ETnfE Each Readineg BE Lnck Enn A Figure 208 11 2 1 Parameters Page GE Imagination at work GE Heqlthcare ParamETtEr Farameters Ligtai Fnst Run Lishay hlit Liegtai Run ntinns ssay Farameters a mple Wawe leneth ll Filename Diata Assay nf J Fieter
57. k Wavelength Reading sample Position Temperature IPDET Statns HT Peltier accessory fitted Indicators below indicator below refers to refer to lamp status heating cooling status SWIFT IT GE imagination at work GE Healthcare Absorbance Mode 1 Abs 2 T 3 Conc 4 Factor
58. Windows Microsoft Excet 3 5 Index 6 6 6 1 GE imagination at work GE Heqlthcare 1
59. nscale 0 w Tnalhar MM AaILAA KA 1 I 1 1 1I i 1 w Gtatuls Bar 1 1 i 1 1 i 1 I i 1 0 900 DO c00 Figure 147 View Spreadsheet flowcell test RA 1 ok a 47 L474 ge 1 5Gg 1 HH dl L147 L474 3 11 ai 121 Ld L470 ce 4 de 4 e474 ge 102 ods 1 LHD7 a 40 Ps 2 4 L474 a ne 1 Lie T1283 L5G L475 ed Ar L4G 74 ad 1 9 1g a 47n cd 4 da 4 L474 an 1 naa 12a LS 4 4 47 a 1 538 1 2 4 dr 4 L474 Hr rr 17 oa 127 LE1 4A ch 4 L474 SH 1 5g9g 1 HH 4 4 1475 4 Hg 119 Hat 1g9 L 1 4 4 ATG Hg 1 Hg 1 L 4 4 4 1 Lae a Et e474 4 ec 5d1 112 lace 1 5 1 47 E FE a 1 3 7 113 19 138 5 14 4 dd drG L474 4 114 Had 134 5 NE ate 4 L474 Tg LAY ae la sls 1 4 1 AG a 4 an L474 4 RT 1 i 13n 17 a 4 Pi E FTP 117 8 13 1 a 4 L474 Ld 11H 1g ld 19 dr L474 BE 1 11 1 LL 1 Fisgure 148 6 5 Time Drive Run Method Data
60. 10 1 2 GE imagination at work GE Healthcare 15 15 AUX 15 15 RS232 RS232 SWIFT Tm 10 1 3 10mm UV
61. Define Slope Region Defaults Function GE Imagination at work GE Healthcare 2 2 File Eile New Method Default M Open Close _ Save Save As
62. No of Assays Wavelength Parallel Mode No of Assays Wavelength Synchronised lag phase Parallel Mode
63. 4 3 Wavescan Display Options Hisnlawr ntinrg Display Qptinns Td bs Inn Gross bs 5 lid Tlicks nnnn Rieset gn nn Fisure 107 7 GEimagination at work GE Healthcare Wavescan View gt Display Abs T hk hisnlay ntinns lp Fgak rea Data Figure 108 ON OFF Peak Find Peak Area Peak Find Peak Peak Original data i jnternnlates Faak Hgjght enerat Easeline Fs Types ctie Ens tty wide arw Wide Trouegh Inflection Fa WWidth Rgsgt Figure 109 Peak Find Peak
64. Graph Results GE Imagination at work GE Healthcare SDreadsheet Overlay Edit gt Merge Display Set up Grid On Off Absorbance Abs T On Off Scale Auto Full Region Zoom
65. New 5WIFT I Time Drive Filg hethods Wem Pin am neT GtTT Btuh Frint Setup higfaul tdm gFL WEggT THD 3 FLO Th Exit Figure 135 File GE imagination at work GE Healthcare Parameters Parameters hietails isplay Run Options hssay Farameters we Filename Data Assay Ne Time Units htt Lnck Temperature Lag Time HH ME Dration Referericg fr one Lse Reterence ark Lse Sample at Start LseSample At Lag Time Ferind Wawe erngth ts lah 5 AM Figure 136 Open Parameters Farameters hgtai hssay Farameters Time Lnits mmss Lag Time hoo Dration D Ferind hoo Waelerngth Fo RE Filename Liatai Temperature mn Rieterehnce re EE Referernce Blarik EE alE Pt EtaT5 EE rhlE nt LE Tine Ta PF ean 8 A Figure 137
66. 1 Slope Quantification Spline Linear Regression Linear Interpolation Tm Kesults Tm 3 3 8 Window Tile GE imagination at work GE Healthcare hethods Edit Wiew Run Fost Run Wi agEaHg hrranges I ons 1 i Graph ICO HI rahh ucnse oxidase mi 9 Graph elucose oxidase 2 d ranh Elucose nxidasg d y Graph gucnse oxidase d 5 Index DT RED jranh ECODSE nridase d Figure 56 Tile Cascade GE imagination at work GE Heqlthcare File Methods Edit View Run PostRun Window Help Re hrrange Icons raP
67. Reference None Blank 6 2 2 Details Page GE imagination at work GE Healthcare Parametarr Lali hisplau Fun Hpiicrm Tilg neralto Cpert Enabled Update all sgaws day HE Fl iamg lenmenter 1E1 3E 1402 gy1zy Figure 139 Title _ Operator Comments Assay Filename Enabled Update all assays
68. Michalis Menten Add Remove Configure File gt Print preview Getup Liefaul Frint ntinns jmdex Graph Spreadsheet Michamlis MMenten Reset iefaults H Header Binchrnm Ltd LF rgnnrt Farameters Hd Cmte Fie mowe uv 9 Figure 121 View IndividualGraphs Overlay graphs Spreadsheet Function Derivatives Auto Slopes AutoScale
69. Run gt Samples Run Samples Run Options Detine Samples dd sample Faminwe sample Filename lear Sahple Riehlicates g ww ppend Figure 166 GE imagination at work GE Healthcare Sample Add sample New Samples Append Filename Sample Title
70. Tile Multi Wavelength 3 5 Application Windows and Views 3 5 1 Main Window 0 0ZRM LMI Flg elhnds wiew Hun Hal Fnr Help press NU 2 Hisure 70
71. GE Heqlthcare Arrange Icons 10 9Help Using Help Index About GE imagination at work GE Healthcare 11 11 1 600nm SWIFT II Cultunre 500ml
72. GE imagination at work GE Healthcare Default User Method 9 Define Method Add Remove User Method DefaultSetings post run Method Michaelis Menten View Michaelis Menten Lineweaver Burke Hanes Woolf BEadie Hofstee 1 Data Set 4 Reaction Kinetcs Run 5 4 1Run Run
73. GE imagination at work GE Healthcare Display Uphons Display Lornternts Difsets pians 5EaT of holrmurm Filter 1 HI ah of Halrniurn filter 2 Cance pl Help Figure 97 hisnla Lnntants tfsegs Dptions Wawelehngth I Higure 98 Contents Offsets Functions IETielie Peak Area ala Figure 99 1 View Display 3 6 8 Slope Reaction Kinetics GE imagination at wo
74. 600 GE Imagination at work GE Healthcare ouam Default aAh etcohnc IDefaul Q es MM ty ethod tanAh wift1 Data Et OF Sd Figure 46 Method Method Data Date Standards Quantification Text DELETE CTRL Find Quantification Multi Wavelength Fraction Analysis GE Imagination at work GE Healthcare 5WIFT I Quantificatinn File hethods Wiem Run Help em ng TTF Find Setup Frint Setup Recernt File Exit Fisure 47 Find
75. 16 64mer gt g ml mol 1 gt pmol 1 9 GE imagination at work GE Healthcare 8 9 Multi Wavelength Post Run Options Multi Wavelength 8 10 Window Tile Cascade Arrange Icons View 8 11 Help Using Help
76. Hile gt Set up gt Print Opions GE imagination at work GE Healthcare Print Preview PC Set up gt Print Options Print Set up Common Print Dialogue Exit 8 4 Method Methods Absorbance Difference Measurement Absorbance Ratio Measurement 3 Point Net DNA RNA Christian Warbergs Parameters Display Result Run Absorbance Difference Measurement
77. ml WIFT I Quantificatinn Stahdard SaITihles LConce Mean SdEr abs 1 AEs2 10000 47 mi1n 20000 994 nm88 40000 1959 n209 Figure 160 Sample View gt Results EY SWIFT Quantificatipn OR SampleTtle No Cone StdEw Mean Cone 1 abs1 oane2_ CT 11 545 1 4 11 52L 114 a CTO 151g gd Hr 13 pPH HG IERTiTI HH Figure 161 2 Standard Curve Standard food dye experiment Conc mg Figure 162 GE imagination at work GE Healthcare 2 View gt Results Samples View gt Display Tabulate Results OFF 7 5 Quantification Run
78. Zoom Auto Full Region Results Michaelis Menten View 3 1 3 1 1 3 3 Unscale Zoom Scale Toolbar Status Bar Mouse Palette 3 3 5 Run GE imagination at work GE Heqlthcare File hethods Wiew Run Hel Detault litt h Ehnd tr Fi
79. Standard Concentrations Quantification Standards Data Quantfication Sample List 3 Sample Data 3 Results 3 Spreadsheet GE imagination at work GE Healthcare 3 Method Standard Sample 3 M S D 1 6 1 Running an Experiment New
80. Tm Tm 1 10 1 1 Figure 193 80 2105 49 Fieure 194 Tm 80 2107 14 gt RS232C Ultrospec 3
81. Add Remove User Method Default Settings derivatives post run Method Michaelis Menten View Michaelis Menten Lineweaver Burke Hanes Woolf Badie Hofstee 1 Data Set 3 3 3 Bdit GE imagination at work GE Heqlthcare GP Wawescan ERK Tima hriwe EH Guantificatinn ia He SWIFT I Reaction Kinetics Untitled File Edit wiew hethod Windomw Help Data Foints EE npy Graph Label WW add Edit hone 20 00a dz g 1 GH Hg da EE Figure 51
82. Next Reaction Kineics 4 3 Run View 2 IFT I Reaction Kinetics sephadex 18 H3 15 1RRE 3 Abort Display Scale Fausel WIW ra Lirtitlgd Fun Ehhadgxx Tntitled 3 000 Feactinn Einetics ssaw RE TT 1 hiuratinn do Wawelengh HH NextReading DOD sarbance 858 Sample lape hming 585 2 000 1 000 ond i WNWIW 13 2 Tirmle rrmr ss Figure 122 Run NN 5 5 Edit Edit View GE Imagination at work GE Healthc
83. SteD Scan SDeed nm minute 1 0nm 6200 3500 2300 0 5 nm 3500 2200 7 1450 0 2 nm 3100 1550 700 0 1nm 1550 SS0 450 4 2 2 Details Page GE imagination at work GE Healthcare aramEtErs Parameters Detais Lishlay Ruh Untinhs Title Uperator Fiorehts Enabled ly Update all samples 5ampnlg Il 9 1 Filename can 1 1 12 3 15 45 a 1 0 Figure 104 Title Operator Comments Filename Date Sample UpdateallScan
84. Display Results Slope Result Quality Full results 5lnne Results Tabulated FT EI 1 glucose nxidasE 1 dl Hbr Lc glucosec niHnsr bh 4 lucose niHaSE HHhD glucose oxidase 4 41 279 Figure 124 SlopeResult Slope Hesulls lgucose nldas Fullresults No Mglhod slpe Resut guaw 1 Manual Fr 1 1 BE 1 Figure 12S Mouse Palette Move 2 5 7 2 Mathematics Assay Point 2 Assay
85. Manual GE imagination at work GE Healthcare Force Zero Spline Imterpolation 2 Linear Regression 4 WAVESCAN 1 Wavelensth Scanning smoothing 1 2 4 overlay 2
86. Grid _ Graph Axis 9 2 3 Run Options Page Parameters Display Fun Options Fost run ranh utoscale Fullscalg Sawe Spreadsheet Sample Handling Frint Repeat Rieterence intinns Samples hafimg hurmher nf cell positions tn be LSE Figure 186 Post run Save Save Spreadsheet Save Spread Sheet Print Print GE imagination at work GE Healthcare Graph Display Graph Autoscale Full Scale Sample handling Repeat reference
87. Repeat bb Temperature GE imagination at work GE Healthcare Samplen of N Details gt Start Wavelength 190nm End Wavelength _ 1100nm Step 0 1nm 0 2nm 0 5nm 1 0nm Scan Speed Ultrospec 4000 6200nm
88. Scan Wavelength Scanning Assay Reaction Kinetics TimeDrive 1 Scan and Assay Absorbance T T Scan Haths Average 3 1E 31H Scan Clear Canstant Kk Canc EF Paned RE Hnlmium Fachlorae 1 1 Hnlmium PFgrchlnrae 2 Hnlmium PFgrchlaras 3 om Figure 90 Scan Maths Assay Math GE imagination at work GE Healthcare Meree Scan Assay
89. Lamp Life Reset 0 Baseline Re Calibrate Cahbration Log FAX calibration log 2 G Check Accessory Diagnostcs Diagnostics report FAX GLP Report GLP status GE imagination at work GE Healthcare Lamp Crossover Wavelength UV 290 380nm 325nm Deuteriuim Lamps Save
90. NotePad WordPad 1 7 yp Enalgd Higure 63 3 4 S Directories au Fieset ethods fwawescan Data files wavescan vn Esported files wavescan adtee are Higure 64 Directories Method Data Liriwe GE imagination at work GE Healthcare Bxported Audit Trail A B 3 4 6 Registration
91. Bdit Edit X RNN 1 GE Imagination at work GE Healthcare Frint Preview Print Setun Figure 77 3 5 5 Derivative View erivative GLP Test 1 LLF wc ILmml Figure 78 Derivative View
92. No of Assays Wavelength GE imagination at work GE Heqlthcare Parameters 3 Parameters hetails Display Run Qptians Mgthnd Save i93 ssaw Farameters Timma Lnits Immss r Lag lim 5 Duratinn nn Perind 5 RiETErEicg Get Reference Eeneat Reference Run Reterehnce Assaw Serial nd Mn nf hesaws Parallgl dg yrchronised 5 Mi wawe Wawelenegth nn TBEmperaturg Enn DSU Fieure 116 IMult wave No of Wavelength Wavelengths No of Wavelength ParamEters Parameters hetails Display Run Qptians Mgthnn Serial gd lo of Wawglengths Parallel hode whchronised ssay Farameters Time Lnits Immss Lag Time mi Duration HI Ferind 1 5 Fieterehnce Get
93. Substrate Concentration Assay Lag Time Assay Duration Method parallel GE imagination at work GE Healthcare Factor Factor Factor Standard 7 2 2 Standards Concentrations Page Parameters otandards Run Options nhntratInhts Lnits igfine Standards Imgyml ama Edit tandard Ranlicatas Fie howe 1 Figure 156 Standard Edit Remove Add Concentration Units
94. Check NR mm mm mm mm Filg Wiem ommand hiataLng He gt y innrnect we ehneth CSS ry i Bn mm am mW bs hode Factni Pieterehnce NG CCBSSS TY Rh jmstrurmEht nrtguratinh Tuhctinrs Caml Figure 26 Configure TT IETF TT BHT Lamp 4 Haurs Rgsgt Easeline Rgcalihrate Gahbration Lng hack Ccessory Liaghnstics LF Rannrt GE imagination at work GE Healthcare Hisgure 27 Configure 2 2 3 Using the Sipper 2 2 2 Setting up an accessory SWIFT I Instirument Contral en Ss Data Log Help N NN N NW N m mm lt enhhenct awBlEhgth ccessory bs hode Rgferehncg herK CCESS TW Li nHTIEUFE Ruh Accessory TumctInhs Do ml Figure 28 Gommunicationn ESaEE Hisure 29 OK
95. Temparature Filename Data 2 1 2 Details Page Parameters Method No of Assays No of Wavelengsth Assays Update all assays GE imagination at work GE Heqlthcare ParaITIEtET Parameters Letalls hiishlay Run ntinns Title nratnr inITITiETts Factor 1 nmcehtratinn TI Lnits Enablsd ly Update all assaws say 1 3 Filename assay 1 24 12 03 15 321 nd3 4 0 AZ Fisure 118 Title Operator Comments Factor Concentration
96. 2000 1 80 2002 70 500 1 80 2002 81 50u1 10 2 Tm Parameters Tm Parameters Details Display Run Options 4 New 4 Parameters lock 10 2 1 Parameters Page Tm GE imagination at work GE Heqlthcare ParaIiEtET Parameters Liatal Fnst Run Displaw Run Cptions ssay Farameters neratinhn Waweleneth lll Haating Heating amp Cooling Temperature LL tn nn Heating Rate i Reterence None Pay Im Before Fun Betore Each Fun Filgnamg Uata IAssay Figure 195
97. 3 7 Reacton Kinetics Post Run Options Post Run Slope Mathematics Derivative Trace 5 7 1 Slope Reaction Kinetics Reaction Kineics Auto Region Kinetcs Assay 2 GE imagination at work GE Healthcare Define Region 2 2
98. Menu Window Window 10 5 Tm Data Edit Copy Graph SNE PIC EN Ee Copy Data Label Add Edit Move 10 6 Tm View Parameters Spreadsheet Overlay Display Set up
99. GE imagination at work GE Heqlthcare Fe Type Fosition 1 Fga ERR FL a Fgak HHr 3 Pek ah Tis 4 Fgak DI 5 Fgak 1 1 al Pek 4 ga nl Fgak nH dj Fgak 1 3d a Pe hah g 1 1 Fgak 4 4 CETIm 11 Fgak hnhh WE Figure 110 Peak Find Peak 0 0 Higure 111 Peak Area Baseline i Ahs HOLMIUM PERCHLORATE a er mn sk Pararmieters 0 lm Spreadsheet Disnlay aa Unscale D manthing 1 0W Peak Find Search I a EE 3 DEriwatrye Detine rea Droapped Baseline Label F 0 000 a 00 0 500 0 Sh Wavelensth nm GE imagination at work GE Healthcare Figure 112 Peak hn Fosition Height tart End 1 dd tal 7 ee EL 2 Hr
100. 3 DNA RNA DNA RNA 260nm 280nmm 3 Point Net 3 3 2 2 1 3 4 2 4 1 2 DNA GE imagination at work GE Healthcare DNA
101. Default Standard Method 4 2 Run time Menu Abort Display Hide Show Run display Show Hide Scale PFull Scale Auto Scale GE imagination at work GE Healthcare Pause Start Reaction Kinetics
102. Index _ About 9 FRACTION ANALYSIS 9 1 Fraction Analysis File gt Find 9 2 Fraction Analysis Parameters 3 GE imagination at work GE Healthcare Parameter Sheet New
103. standard 1 1 7 Security Facilities 1 7 1 Parameters Lock NR OO SWIFTT 1 7 2 Audit Trail Audit Trail
104. Parameter lock 4 6 2 1 Parameters Page GE Imagination at work GE Healthcare FalamElErs psik isnlap Hum ptians FilEr ri ass gmihEfature nr 1 Hekeience 3sdW Fa 3 Time Uni hhmmss Lag Te cnn Lurahnm Iinm F Im Immm anslan thls nn g Figure 138 SO Time Units Lag Time Duration Period Wavelength s 3 n of N N 3 1 2 3 Filename Data ore se Fence Blank sp ample St saeSampls ht Lag Time Temperature
105. 1ml Common Menu Descriptions 3 3 Post Run Menu Figure 2060 11 2Culture Parameters 4 Parameter sheet
106. Peak Find Auto Scale lb Defaults Function 6 3 Time Drive Display Options File hlethods Edi Wiew Run Fost Run Window Help ne a a py breadsheet er law la k Lnscalg w InnIhar w tatus ar w house Falletg GE Imagination at work GE Heqlthcare Figure 142 View View gt Details Disnlay Options Liisnlay ntinhs IIHH T hone Dross 5nlid Ticks wi Format Higure 143 Graph flowcell test AAA 2 mk Abs flowcell test AAA 2 1 0Q0 Q 8QQ Oo 600 oOo 400 0 00 20 00 40 00 60 00 80 00 Time Min Figure 144 Grid
107. Remove Wavelength Equation List E quatprmri Etni 4 it Cabat Equaticn 12 259 bBs 511 HH 2 bs 1HL FrenisrrinlrBsLI ml mi ed Fiesult Cancel Figure 173 1 Add Equation Equation Editor 2 Tidle Title 3 Constant Wavelength GE imagination at work GE Healthcare Constant Wavelength Select wavelength Wavelength At Wavelength Value Wavelength Parameter Abeorbance bt awelength IEanstarl OE EEE rt absnban
108. GE imagination at work GE Heqlthcare af 1 ear nstant Figure 126 Assay Math Merge Assay Point Point Maths 360nm 14 MP PR GE Imagin
109. mdex Merge IFT I Reaction Kinetics ndex SEPHADERRED RE DI 1 File hiethods Edit ismw Fn FastRuh Windom Help N2 el ephadesx Figure 22 Merge Delete Result View Cut CODy Paste 0O Copy Graph 2 Data Results GE imagination at work GE Heqlthcare Label Add Bdit Move
110. 2 3 GE imagination at work GE Healthcare nposition changer n reference 1 9 3 Fraction Analysis Display Options Lishlay TI nng Ps Solid Tcks Figure 188 Fractiion Analysis view gt Details Graph Results ON OFF NGG 9 4 Fraction Analysis Data
111. 3 4 8 Spreadsheet 3 4 9 Default Windows WS RK TD Tm 3 4 10 Default Windows QA MW FA 3 5 Application Windows and Views 3 5 1 Main Window 3 5 2 Group Tree View 3 5 3 Data View 3 5 4 Spreadsheet Data Points View 3 5 5 Derivative View 3 5 6 Results View GE imagination at work GE Healthcare 3 5 7 Sample Results View 3 5 8 Overlay View 3 5 9 Michaelis Menten View 3 5 10 Run View 3 6 Post Run and Other Functions 3 6 1 Smoothing 3 6 2 Tm Calculator Tm 3 6 3 Mathematics 3 6 4 Peak Find 3 6 5 Peak Area 3 6 6 Derivative 3 6 7 Overlay 3 6 8 Slope Reaction Kinetics 3 6 9 Slope Quantification 4 WAVESCAN 4 1
112. Standard Replicates 7 2 3 Run Options Page Farameters Standards Puh Cptions Fnst run amples Handling av Fepeat Rieterehnce Gawe obreadsheet mp es E _ Frint Options 0 humber ot cell nnsitinns tn be Figure 157 GE imagination at work GE Healthcare Post run Save Save Spreadsheet Print Print J Sample handling Repeat reference 1 Repeat reference OFF MA IO Samples n
113. Assay Assay Update all assays 6 2 3 Display Page GE imagination at work GE Healthcare Paramelers hietals Display Hun Hplinrs llglault mg Unts Ii Zw uratinn mmis Enable le 00 sabance HH BE Minimum PErermEhtal 5 yg FF Enchle TU Figure 140 Default time units Minimum Abs 0 0 3 0 MaximumAbs 0 0 3 0 IncrementalSave 2 Initial axis duration X
114. Assay Parameters Wavelength Temperature 20 105C Heatingrate 0 1 3 0C Cooling rate 0 1 2 0C Assay Filename Data Assay Hilename Operation Heating Heating amp Cooling Delay Reference None GE Imagination at work GE Healthcare Before Run Before Bach Run 1 10 2 2 Details Page
115. DNA 10 mm 260 nm 1 0 S0 ug DNA conc Abs 260 0 50 DNA purity Abs 260 0 Abs 280 0 RNA RNA RNA 10 mm 260 nm 1 0 40 xg RNA conc Abs 260 0 40 RNA purity Abs 260 0 Abs 280 0 Christian Warberg 280 nm Christian Warbers 280 nm 280 nm
116. 1 Repeat reference off FT CT Samples n Repeat reference on 1 play Fun Uptions ranh utnsrale Fullscalg saHshegt Sample Handling Pepeat Reterehce w ITIDlEs FE Lietirne hurmiher nf Samples per Reterernce hcluding RgferEhcg Figure 187 Samples
117. 2 1 2 Dynamic Data Exchange 2 1 3 Specification 2 2 Set up 2 2 1 Setting up the instrument atter software installation 2 2 2 Setting up an accessory 2 2 3 Using the Sipper with SWIFT II SWIFT II 2 3 Command 3 3 1 GE imagination at work GE Healthcare 3 2 Running in General 3 3 Common Menu Descriptons 3 3 1 File 3 3 2 Method 3 3 3 Edit 34Wiew E 3 3 5 Run 3 3 6 Calculator 3 3 7 Post Run 3 3 8 Window 3 3 9 Help 3 3 10 Run tme Menu 3 3 11 Mouse options 3 4 Set _unp 3 4 1 Print Option 3 4 2 Line Styles 3 4 3 GLP 3 4 4 Audit Trail 3 4 5 Directories 3 4 6 Registration 3 4 7 Communications
118. Exit 9 1 4 2 Typical Display Multple Document Interface MDD GE imagination at work GE Healthcare lennBar Toalbar Tool Falette Index View roup ox Ee LP Test LP Temsk i LP Test 2 BLF TgsE3 npreadsheet View Graph view Overlay View Status Bar Figure 1 1 4 3 Rigit Mouse Click Windows Windows 3 1
119. Auto Read 2 2 1 Setdng up the instrument after software installation Biochrom Ltd 2 2 Registration 2 2 Communication 2 2 Communication Set up control Panel Command Connect GE imagination at w
120. Equalions halals Fun Options Fashm Banmnlmnandlhg 9 RapaatHalerense Save Spreadsheel ale BE Frnt ct Diefirne umhg of cell postiors to be I FIT Figure 176 Post run Save Save Spreadsheet Print print Sample handling Repeat reference 1 Repeat reference OFF 1 Samples n
121. GE imagination at work GE Healthcare IMETHYL HK Eade Hfsee Fg ge3 HII LEwEawB ELurk ED eT 6 Hares Wennlf FE ee ha 1 I ETn hn Print Figure 133 Michaelis Menten All View Results gt All Lineweaver Burke Badie Hofstee Hanes Woolf 8 3 Overlaying results Lineweaver Burke Michaelis Menten 9 Window Tile C I Cascade Arrange Icons View 5 10 Help Usi
122. Main Toolbar Main Toolbar SWIFT II gt Windows gt SWIFT II Parameter Data Method Windows gt gt gt OFF GE imagination at work GE Healthcare F1 OFF NEVBR
123. Sample Replicates New Samples Run Samples Pun uptinhs Fnst run ample Handling le BwE Hepeat Raferehici Saw Sbreadsheet IThles E Frint Cietire humber nf Samples per 6 Ptions Reterence hcluding REfErEhcg Figure 167 Rinnineg Samples Pianrning Sample le Feplcate bs IIIH hean bs OO nc EOD GE imagination at work GE Healthcare Fisure 168 7 5 3 Use with Sipper
124. 4 Parameters Details Display Run Option 1 5 2 Running an Experiment
125. Hefine New Samples a Imhla ames 5a mple mher nf samples Clear existing samples Oreel Figure 192 Sample List 7 GEimagination ot work GE Healthcare Runnine Fractions E34 Figure 193 9 5 1 Use with Sipper
126. Method Data Imdex Method Parameters Save Data Wavelength Scanninsg scan pen Lnnk In aha EF GLP Test D1 02 96 15 28 SD LP Tgst HE Hg dE 11 211Wy5h iLF Tegst HU H1 dE 1E dH eS Filg hame higlaul Files of tupe Data FD Carncel Figure 6 Windows Data Method Spreadsheet 1 5 1 Setting up Parameters Method
127. Print Preview PC Set up gt Print Options Print Set up Common Print Dialogue Exit 10 3 Method Default SWIFT II Method G GE imagination at work GE Healthcare User Method Method 9 Method Define Method Method Add Method Method Remove User Method Default Setings post run 10 4 Tm Run
128. View Scale label be CO TILT NE CO View Display 3 4 2 GE Imagination at work GE Heqlthcare Post Run Wimdow Cascade Tile rramge Icons Toolbar atahns Bar hiouse Palette Figure 75 3 5 4 Spreadsheet Data Points View Figure 76 OE
129. Parameters Graph Results Spreadsheet Overlay EditzMerse Michaelis Menten Plot Michaelis Menten Plot 5 8 Michaelis Menten Display Set up Grid On Off
130. Find File earch Fattern hirectnry Cs Frogram Files slF TT Wuant hce Fieure 48 Find Close SaVe SaVe AS Method File Data File Standard File Spreadsheet File Export Wavescan J CAMP Mult Component Analysis Set UD GE Imagination at work GE Heqlthcare 3 4 Print Group Report Data Report Spreadsheet R
131. Deriwatiwg Farameters LiEr yatiyg EN Offset nwnlutinn First Deriwatiwe y EE Widg tr hedium a Narrow 5 Figure 202 Display Original Data First Derivative Offset Convolution Wide Medium Narrow x Typical Melting Curve Temp CO Figure 203 10 7 3 Smoothing GE Imagination at work GE Heqlthcare Figure 204 10 7 4 Trace SWIFT Figure 205 Trace 10 8Window Cascade Tile
132. Substrate Concentration Standard Sample File gt Find Standard 20 Sample 300 linear regression liner interpolation spline man
133. 1 4 Programme Style 1 4 1 Main Toolbar SWIFT II METHOD Main Toolbar 9 Instrument Control Main Toolbar SWIFT II gt Windows gt gt gt SWIFT SWIFT II Main Toolbar Always on top Autohide Autoload Windows About
134. Accessory Accessory Sample 1 Next Sample Enter ell Pnsitinn 1 Figure 33 Pelter Pelier 20 50C 20 105 C Tm Absorbance Mode Abs Mode J Absorbance Transmission Concentration Factor Reading Statuns Command
135. Method Data 7 1 Run gt Standards WIFT I Quantificatinn DYE 24 12 n3 13 File ethnds Edit leW Fun lape mimidnm Hel Standards aapbles Figure 163 Run NN CE tandards Rnrning Standard 2 Feplcate bs HHH Feplicate 2 bs HHH gan bs OO Figure 164 GE imagination at work GE Healthcare Standard food dye experiment 20 0 Conc mg Figure 165 7 5 2 Kun gt Samples Samples Parameters
136. 1 2 4 Bnhanced X 1 3 6 7 Overlay
137. 3 6 2 Tm Calculator Tm Tm Calculator Tm My hmnlyul L Rl TAAAACGACGGCCAGT_ ee a E5m re ii nm mal 2 Clear Lielete CFMA DN lass Figure 89 Tm Tm Multi Wavelength Tm Tm RNA ssDNA T U 16 64mer ug ml mol lt gt GE imagination at work GE Healthcare pmo1l ul 3 6 3 Mathematics 2
138. Define Samples Define New Samplese GE imagination at work GE Healthcare Derfine He SampEs 5arnhle arnels ls Murrher of sarples Ear ssshna samples Carnce Figure 180 Sample Name s Number of Samples Clear existing samples Add sample Remove sample Clear All Append Run Option Parameters Run Option Parameters OK GE imagination at work GE Healthcare Funning SgmmmDHEs Hiurrin 5 mFla lgsHH war nh 2 hs 4 010 a Enh gdHH hs 3 Figure 181 Run dialo
139. SCale Full Scale Auto Scale Pause Start Reaction Kinetics Next Reaction Kinetics 3 3 11 Mouse options jm mm Figure 58 Zoom Label Move Label Offset Peak area lrace
140. 6 2 4 Run Option Page Parameters Details Display Fran Uptons Past mn le Funchon Fe Ed new awe 5prea heel Herlau Hranhs PP Fird Fni Spreadsheg ly uln Bcale Frint Uphors Lalaull sethngs Figure 141 Post Run GE imagination at work GE Healthcare Post Run Save Save Spreadsheet Print Print View Individual Graphs Overlay graphs Spreadsheet Function Derivative
141. Registration CS ET GLP Report GLP Report PC Set up GLP Print with Data Log Set up gt zGLP GLP Parameters 00 1 2 Graphs Results
142. Display Uptinhs Display Contents ffsets Options fF Fnrmat Mnng Pranss Sol Ticks ON OFE GE Imagination at work GE Healthcare KS Derivative Slope Lines Multi Point Breaks Offset Axis Lines Scale Auto Full Region Zoom Zoom Auto Full Region Unscale Zoom Scale Toolbar Status Bar Mouse Palette 6 2Results Michaelis Menten View 3 1 3 1 1 3
143. el d bl 7 1 575 5 1149 bh a 4d d 4 hb ee LO 5 1 HL 4 lh al al g FI 17 11 nl dd 4 HB 4 1 ag PF sl dh 1 g1 1 dl RH 1 4 gd 1 g da 11 HH ag IE Hg he Figure 113 Peak HOLMIUM PERCHLORATE 0 0 Figure 114 4 4 Wavescan Data 3 GE imagination at work GE Healthcare Abs T 4 5 Wavescan Run Method Data
144. Factor GE imagination at work GE Healthcare IFT I Instrument nntrnl File Wiemw Comiand bataloe Help Wi Wawelernegth 36G0 CCEsSOry 1 Ee hs hode Referen Factar OC GTce Factor Check hccessory Fiead Next Ue nlgurg sgt Factnr Cioncentration IGam 4 Figure 34 Factor TE Fartnr mw Figure 35 Factor Reference Configure 7 GE imagination at work GE Healthcare fe haulemrlum Lamp 4 Hanurs anor Lamn Haurs Tunnsten Lamp rd Hnurs Hgsgt Baseline Fiecalbrate Calbratiorn Lcn Check ceessory Lianrnshics iLP eport Lamp Lrnssnwgr rengIh Jp Fieset Baseline Fecalbrate alhrahinn Lnn Check ccessnrn Liannnshics iLF Fieport WF Deuterium lanp sawg Figure 36 Configuration
145. Conc Factor 2 2 1 2 Dynamic Data Exchange Instrument Control Panel Dynamic Data Exchange DDE Excel Word Help Files DDE 2 1 3 Specification Instrument Control Panel Wavelengthrange 190 0 1100 0nm 190 0 900 0nm Temperature range 20C 50 C 80C 105C Absorbancerange 3 T 1 2 2 Set up
146. View Results Results data Time Hasg Factnr EK 1 lt Iauto select II 1 OL 1 ILH 18 1 2 al 1 HUH 1 1 3 RIs 1 IUH TH 1 32 TL 1 IZH TD 5 1 5H 1 HUH 1 g TT 5 1 IUH Td 1 ed 1 IUH 1 1 Bl Em 1 HLH 1 48I 1 38H 1 1 1 477 4 1 BI TUH 1 487 4 11 ee 1 HLH ET 1 4 1 Figure 217 11 5 Culture Run SWIFT I Culture File hethods Wiemw Fn Help Detault FR thod tr Last THL GE imagination qt work GE Heqlthcare Fisure 218 Run Run Default Method Last Default Parameters U HEHEHEHEHEHEHE E E E E E EE Parameters Detai Fost Run Lisnlay plit Letais Run Cptions ssaw Parameters a mple ve lngth BU _ Filghame Data IAssay nf Reterence ee rone Before Each Run Bafare Each Reading Enn Figure 219 Method Method M
147. 3 Parameter lock 9 2 1 Parameters Page Farameters Display Run Options Trtlg perator Cinients Filenams Assay 1 Wawe lenegth Ben Temperature Figure 184 Title Operator Comments Filename Date Wavelength Temperature ER 9 6 9 2 2 Display Page GE imagination at work GE Healthcare Parameters Lishlay Run ntinns arid ranh x Fr Monei Mross Line fbs Salid Ticks ar Peset Figure 185
148. GE imagination at work GE Healthcare Remove User Method 8 5 Multi Wavelength Run Method Data Run gt Samples Run Samples Sample Add sample Define Samples HLn 5 mnles HLr Hpiinrs Dafins 5amplss dd sample Hemowe sample Eikename tes Llear Er Hisure 179 Filename Sample Title
149. Microsoft Windows User s Guide 1 2 Installation of Software SWIFT II METHOD 10 SWIFT II METHOD Microsoft Windows 95 98 2000 PC CD ROM Welcome Next C PROGRAM FILES SWIEFT II Browse Next Set up Typical Next Microsoft Windows NT PC CD ROM
150. Save User Method Method 2 1 1 Parameters Page ParaITiEtET Tprameters hetai hisnlay Fan ntinns ssay Farameters Mgthnd Time Lnits EES Serial hode No nf ssaws Lag Time PFarallgl hode Luratinn 5Swmichrnnisgd Fgrind Multi wawe Wawelergth nn Rieterehnce Get Reterence Reat RETerernee HaiiidfaNfs 0 Ruh HEfErEcE Assay Filgname Eata Issay 9 Figure 115 Method Serial Mode 1 CT lag phase 1 GE imagination at work GE Healthcare
151. A325X6 815 A334X4 260 A310 X GE Imagination at work GE Healthcare 2 555 j A325 Cobalt and INickel determination Cobalt g l 12 259X AS11 0 302 Xx A720 X dilution factor Niclel g l 0 403 AS11 27 407 x A720 Xx dilution factor File gt Find GE imagination at work GE Heqlthcare 8 2 Multi Wavelengsth Parameters Equatons Details Run Opions 3 New 3 Parameter lock 8 2 1 Equations Page Farameters Equations i
152. Data Points GE imagination at work GE Heqlthcare Form Feed Text 1 Cnn nn Ffbu f b u 3 4 2 Line Styles Fieset Defaults Display Figure 61 Line Style Colou
153. GE imagination at work GE Healthcare Define Define Autoscaling Peak Hisure gt 1 5 Wavescan Reaction Kinetics Time Drive Culture Tm Method Data Spreadsheet Bxcel
154. a Command Accessory Pump Parameters GE Imagination at work GE Healthcare Pumn Farameters I nluime 5 Mode Full nlume 5 Haturn ettle time 1 lbrate ash Figure 30 Mode Waste Return Sip Volume 0 1 60ml Pull Volume 0 0 60ml Settle Time 0 0 10 0 Calibrate 10 120 Wa
155. Edit Spreadsheet View Sample List View Data Points Michaelis Menten View Clear Group View List Michaehs Menten View G GE imagination at work GE Healthcare Sample List Results View IMerge 1 View Overlay
156. Fe Wiewm Command DatsLan Help Alc Co binrbances od fr abs Conc Factor EE 1 MM ga Fnr Help pess F1 IEam2 Figure 7 Instrument Control Panel Instrument Control Panel 2 2 2 3 2 Ua 2 1 1 Programme Appearance Instrument Control Panel Title bar Instrument Toolbar Status bar Application bar Dialogue Box Title bar Instrument Enntrnl Fisure 8 Instrument Control GE imagination at work GE Healthcare Instrument Toolbar IA slwl Figure 9 Instrument Control Toolbar Instrument Control Toolbar Set uD Connec0Disconnect Set Wavelength Absorbance Mode Set Peltier 1emperature Set Accessory Cell position 1 Next cell Configure Log Reading Print About Help Status bar 4 For Hetp preseF1 Ca
157. Run gt Samples GE imagination at work GE Healthcare Run mamples Display Run Dptions Detine Samples dd sample Farmnwg sample Filgname lear le Dpehnd Fisure 191 Samples Add sample New Samples Append Filename Sample Title New Samples
158. 2 nm Tmin n Betore First Scan cd4H hin Betore Each Scar T1856 nm min Fisgure 103 Common mdividual 2 Sample Sample n of N N N Reference None Before First Scan Before Each Scan Filename Data Method Standard Repeat
159. Enter Lockineg Password Wm EC Figure 41 File Default M GE Imagination at work GE Healthcare TE SWIFT I Reactinn Kinetics Fi hiethods Wiem Hun Help em beh TH BEtUh Frint Setup PRUGR FILESTSGWTF 1 TE TCSNDEF LT RK 9 Frngram Filgs MTFT FFKinetics ssay 7 12 03 14 27 RED Prngram Filgs WTFT FEKinetics ssay 17 18 03 14 84 RE PRUGR FILEGWSGWTFT FEINE TI CGNG T RKD Eit Fisgure 42 Default M file WIFT I Reaction Kinetcs DE File hethods Edit Wiem Hun Fost New GtrN ne fr nse de wB 3 etup Frint TTFF Frint Prewiew Frint Setup 1 DEFALULT RE 2 ssay 7 12 03 14 27 RED 3 ssay 7 12 03 14 24 RED 4 TT RED Exit Figure
160. None Solid GE Imagination at work GE Heqlthcare Graph fiomwcell test mn 2 Ahs flowcell test AA 1 0Qnn 0 8Q0 0 600 0 400 0 00 20 00 dQ 00 GO OD SU D0 Time IMhm Figure 145 Option Derivative Peak Find Peak Area Offset Axis Lines Display Options E34 isplaw ntinhs EriratirE ata ly Peak rea Data Offset ig Lines e Figure 146 6 4 Time Drive Data GE imagination qt work GE Healthcare File gthadg Edit Wem Rm FnstRun Windom Help 0 ee ws Graph flowce Spreadsheet a xX Cer aw flowcell test AAA 2 pa 1 000 6 lp k ee MG LS ot a eee ede ee et mF er oe i ecc EE Ee rR
161. 4 2 Wavescan Parameters 4 2 1 Parameters Page 4 2 2 Details Page 4 2 3 Display Page 4 2 4 Run Options Page 4 3 Wavescan Display Options 4 4 Wavescan Data 4 5 Wavescan Run 4 5 1 Standard Mode 4 5 2 Repeat Mode 4 5 3 Use with Sipper 4 6 Wavescan Post Run Options Reaction Kinetics 5 1 2 Reaction Kinetics Parameters GE imagination at work GE Healthcare 5 2 1 New 2 1 1 Parameters Page 5 2 1 2 Details Page 5 2 1 3 Display Page 5 2 1 4 Run Options Page 5 2 2 File 5 3 Method 5 4 Reaction Kinetics Run 5 4 1 Run 4 2 Run time Menu 4 3 Run View 5 5 Edit 6 Reaction Kinetics Display Option 6 1 View 6 2 Using Michaelis Menten V
162. Frint Spreadsheat ly tn Scale Frint Optiors Cietault Settings eS RS B Figure 106 GE imagination at work GE Healthcare Post Run Save Save Spreadsheet Print Prnt View Individual Graphs Overlay graphs 9 Spreadsheet Functons Derivatives J Peak Find Autoscale Defaults Settings Function
163. MinimumAbs 0 0 3 0 Grid 10 2 4 Run Options Page Faramgters Detak Fast un Display Fiun Uphons Pot iLm En Furnchon ly Gawg ly IndwidLial Hrsphs igmwahws ave Speadsheet Fieadsheet Fe luto iene region Fint Defne len run Fri phinns gral I seihos Figure 198 Post Run Save Save Spreadsheet Print Print Option View IndividualGraphs GE imagination at work GE Healthcare Spreadsheet Function Derivatives Au
164. RK TD Tm In WeinHnw Hun applicahinn full screen Minimized or Inad Fun window Full size Fe Minimize nn run Minimize nn lost focus Hieset Figure 68 WS RK TD Tm Imdex Window new load run lose focus post run 3 4 10 Default Windows QA MW FA un application full sereeri Lniad Lnninurahinnm Fun Lnnlinuralinm FF Show ranh FShow Graph Show Results Show Results Tile wiews Tile Wiews Figure 09 QA MW FA GE imagination at work GE Healthcare
165. 43 New Save OK GE Imagination at work GE Heqlthcare Open i WIFT I Reaction Kinetics File hethods Wiew Hun Help IgE WE neh tr etup Frint metup 1 ssay 9 12 03 15 47 REKD DEFAULT RE 3 say 712 3 14 27 RED 4 ssay 7 12 03 14 24 RED Exit Figure 44 OPEN ED kinetics eoxid1 RKD SEPHADES RKD sephadaxRKD RKh Ipats RK mw Hisgure 45
166. AT1 27 Figure 100 GE imagination at work GE Healthcare 5lnne Hesulls x laucose nlHas 1 Fullrasuks LNo_IMehod_ spos_ He Leeb 1 Manual Fit 1 1 BE Figure 101 Mouse Palette Move efeitos NEAT CC 3 6 9 Slope Quantification Quantification pe lt Spline 3 S 5 Linear Regression 2 Linear Interpolation
167. Err ly Cell Ech i shncel Figure 222 Pin Fost Pin ww gthnd Gr R 5nlit I sg Figure 223 Run gt Method OT Run gt Method Method ee Method Last Method Split Split Data Box Leap Lulture 1 Farinr 1 II Urs He__ i ET spe fes 1 HHH DOO NNN slart fime T1810 11 1513 Points 1 huralinn ODE 44 2 IEITI x H ll HT FD IMUIHII TnmluE msss Gtart time 1BJHHJEH 11 1E13 Fam 1 hiurahnn OO OE a4 GE imagination at work GE Healthcare Fisure 224 11 5 2 Split No Facn _ EE Al U
168. Page 9 2 2 Display Page 9 2 3 Run Options Page 9 3 Fraction Analysis Display Options 9 4 Fraction Analysis Data 9 5 Fraction Analysis Run 9 5 1 Use with Sipper 9 6 Fraction Analysis Post Run Options 10 Tm MEBLTING POINT Tm 10 1 10 1 1 10 1 2 10 1 3 10 2 Tm Parameters Tm 10 2 1 Parameters Page 10 2 2 Details Pase 10 2 3 Post Run Display Page 10 2 4 Run Options Page 10 2 5 Tm File Tm 10 3 Method a GE imagination at work GE Healthcare 10 4 Tm Run 10 4 1 Run Parameters 10 4 2 Run Time View 10 5 Tm Data Edit 10 6 Tm View 10 7 Tm Post Run Options Tm 10 7 1Tm Calculation Tm 10 7 2 Derivative 10 7
169. Reterence Eereat Referernce Waarelenegths Temperature Enn Filename Data Assay RT HETETECE nessa fy GE Imagination at work GE Healthcare Fisure 117 Multi wave Assay Parameters Time Units Lag Time Synchronised Duration Period Synchronised 15 Reference Set Reference Serial Repeat Reference Run Reference Parallel Synchronisesd
170. Titte _ Operator Comments Assay Filename Enabled 9 Update all assays Assay Assay Update all assays 11 2 3 Split Details Page GE Imagination at work GE Heqlthcare ParametErs Farameters ietai Pnst Run hisnlay Splt Letalls Run Options le Enable Split Titles FEInal tetcfc Snlit Size Split 1 plit 2 Fisure 211 Split Details Split Size Original Assay Sp
171. 1 1 FUOPHtHmmLrdttU C wnt File Methods Edit View Run Slope Window Help es Linear Interpo lation hanual Result Force Zero Extrapolate curye Figure 170 8 Multi Wavelensth 8 1 Multi Wavelensth Absorbance Ratio DNA RNA Absorbance Difference 3 Point Net Multiple Wavelength QC of batch vitamin A purity Parameter
172. 3 Smoothing 10 7 4 Trace 10 8 Window 10 9 Help 11 11 1 11 2Culture Parameters 11 2 1 Parameters Page 11 2 2 Details Page 11 2 3 Spht Details Page 11 2 4 Run Option 11 3 Post Run Display 11 4 Culture Data 11 5 Culture Run 11 5 1 11 5 2 Split 11 5 3 Close GE imagination at work GE Healthcare 1 1 Microsoft International Business Machines Corporations SWIFT II Windows 93798 2000 NT SWIFT II METHOD SWIFT II SCAN WS SWIETII KIN RK SWIFT II TIME TD SWI
173. 9 Default tmeunits Minimum Abs 0 0 3 0 Maximum Abs 0 0 3 0 2 1 4 Run Options Page Paramalsrs bestals hisrlar Hun Hpinrms Fost TLTI HB FuriEII r aw ww radiwictaal rapts Dermatiwe SavetSpeadshes Dvelar Graphs Fr tuto Scale Fiinl picadshesl Fri pls Fr ut Ins Hal ope ren r I TO aault Gethrngs Figure 120 Post Run GE imagination at work GE Healthcare Save Save Spreadsheet Print Print Option Print Option
174. B34E Figure 15 Registration GLP Getun tDefault 5chemes Rgistratinn Uommunications Spreadsheet Language GLP hata Lngging lstrurmieht Eterhal Peltier Cam1 Com3 MV Danmg Gomi nm Com _ _ Dom 2 Gnm 4 De monstration Librars Lheck tor External Feltier K Fisure 16 Communications Chec
175. Define Region GE imagination at work GE Heqlthcare 0 Copy Data Autoscale Peak Area dropped sloping Peak Search Slope Region 3 4 Set up rg Default Line Styles LP uHit ra Directories hasfau Windos he mes Ragistratinh nHTLTICatI IS breadsheet Larheguaege Frint Options Default awe S Lig et Fw Figure 59 Setup Set up
176. FT II OUANT QA SWIEFT II MULTI MW SWIET II FRAC FA SWIFT II Culture CU SWIFTII Tm Tm Tm Instrument Control SWIFT II ISO9001 PC PC PC GE imagination at work GE Healthcare PC Microsoft Windows 93798 2000 NT Microsoft Windows
177. GE Healthcare SWTFT II Version 2 0 169 0073 3 25 1 Tel 03 5331 9336 Fax 03 5331 9370 GE GE imagination at work GE Healthcare 1 1 1 1 2 Installation of Software 1 3 Helpful Hints 1 4 Programme Style 1 4 1 Main Toolbar 1 4 2 Typical Display 1 4 3 Mouse Click 1 4 4 Status Bar 1 4 5 Toolbar and Mouse Palette 1 5 Wavescan Reaction Kinetics Time Drive Tm 1 5 1 Setting up Parameters 1 5 2 Running an Experiment 1 6 Quantification Multi Wavelength Fraction Analysis 1 6 1 Running an Experiment 1 7 Security Facilities in SWIFT II SWIFT II 1 7 1 Parameters Lock 1 7 2 Audit Trail 2 2 1 2 1 1 Programme Appearance
178. LP Never Always Daily Weekly Audit Trail Always GLP statuns Setun Defau Che mes Registratinn Cnmmunicatinnhs Spreadsheet Languaga LF ata LnggIig wt Re 2 Figure 22 GLP GE imagination at work GE Healthcare sgtun Default Schemes Rggistratinh UnITILTICatIn breadsheet Largluage aLFP Data Logeineg ample Text Sample lin Sample Mumhmr 1 ly auto Read Clear contents gt Ssh Pit IDataLng Lag Figure 23 Data logging Auto Read ep Reading
179. Michaelis Menten Lineweaver Burke Hanes Woolf Eadie Hofstee 4 Overlaid GE imagination at work GE Healthcare ile Et VEW Miethod Window Michaelis Menten Graph Display rad Fadie Hofstee Display Imtercepts Lineweaver Burke Toolbar Hanes Woolf tatus Har lnmnse Palette Fisure 131 8 2 Viewing results View gt Results gt Selected Km Vmax Hill Eo Kcat Vmax Eo Kcat Km Eo Resulls Michaslis Msgnlan Eade Holshee Lin wa BT Buika H rar aa ETHTL HE4 had E adic Hufstes lnne HglT Intercgnt H ualiy 1 HIH Em E ay d LH Hill cpatt 7 ct 15 KcalErn 833g Elg 5 mm Figure 132
180. ant Gtore Fesult Hawlmm Hgsults Figure 91 Store Result Review Result Print File gt Print Abs260 x 50 Abs260 280 GE imagination at work GE Healthcare J 3 6 4 Peak Find Peak Search Default Setting Search Peak Find Parameters 5Earch Diginal data Internulale Feak Heinhi Generale Baseline Trnuah Inrschian Paak width Reset Eahca hn Help Figure 92 Peak Search Peaks Troughs Inflection Points
181. are Index View Clear Merge assays 1 View Overlay Group Meree Copy Paste IM 0 IM N ET Cut Graph View Clear Copy Graph Copy data 6 Label Add Edit Move Spreadsheet View Edit
182. ation at work GE Healthcare lH Save Del Ec Llgar s Corstant Gtore iesult Bev Hgsults Fignre 46 Point Maths Dialog Bo Figure 127 Store Result Review Result Print Hile gt Print 7 3 Derivative 1 EE heriwatiwe Farameters LEr iwatlyg EE Offset Ln Pnlutin First Deriwatiwe Wide hedium Narrom 9 A Figure 128 1 GE imagination at work GE Healthcare
183. eak Find Peak Area Derivative Trace GE imagination at work GE Healthcare Fnst Hun Windam Help athematics a Fe Find k Pi Fea rea k a Lasriwatiyg Trace Figure 152 Post Run Math Fnst Ru Mindow Help Mathematics glwgl Fs Find F Gearch PFgak ra Rgsults Lerrwatiye ear Trace F Figure 153 Post Run Peak Find Mathematicg FE ty Peak Find GE Faak ra FW Search Lasriwatiye Detine rea Fk LDropped Baseline Trace Fesults lonping Easeline aT we Figure 154 Post Run Peak Area GE imagination at work GE Healthcare 7 Quantification 7 Quantification 3 Factor StandardCurve Standard Sample
184. ees gl wor RT Figure 174 Select Wavelength in Equation Editor Wavelength At Waveleneth Value Wavelength Parameter Wavelength Result Fe Clear Back Space Precision of result Bquation Editor Cobalt g l 12 259 A511 0 302 A720 100 1 Equation List Add Equation 2 Title Cobalt sg GE imagination at work GE Healthcare 3 2 4 Constant 12 259 6 Wavelen
185. ehce r Mone Bsfanrg Each Run Betore Each Reading Figure 209 Wavelength s Sample 24 3 nofN N 3 1 2 3 Details Filename Data References Temperature 4 11 2 2 Details Page G GE imagination at work GE Heqlthcare PFarameters Ed Farameters Details Fnst Run Lisnlay Shlit Ligtails Run ntinns Title neratnr niITEHts Fartor HI Lnits Ernabled Update all assaws fssay a 3 Filename Iassay 1 2 18 H3 17 4 1 3 a Fisure 210
186. en 3 5 10 Run View Figure 87 Run View 3 6 Post Run and Other Functions 3 6 1 Smoothing GE imagination at work GE Healthcare moothing EE Filter Flot Step an Charhge nL None Mn hanng idg hgdium Narrnw Cance Figure 88 Filter plot step
187. eport Set up gt Print Opions Print PreView PC Set up gt Print Options Print Set up Common Print Dialogue Exit 3 3 2 Method er File kethads view Run Heh Detault Ff 1 ser hethod Detine hethod Detault settinegs Figure 49 Default Default Filg Ethndg Wiem Calculator bs Difference Ctr1 nbs Ratin CfTHg a Ft Net tr bin tr RI tr TB CG Warbure TH ser gthnd Liefimm hethod Figure 50 Method GE Imagination at work GE Healthcare User Method 9 Define Method
188. eqlthcare Hisgure 213 View Display je Fun Fost Fun Farameters Results Display Leegehd d le Llnscale w Innlhar gtatus Bar w house Fallgtg Figure 214 Display 11 4 Culture Data ParametErs 3 Farameters Liatalls Pnst Run Display Split Details FMun untinns aITinle Handlimg ho nf samples tn be Included ner cell Er aranlg chaneger ly hn Results Figure 215 Run Option Show Graphs GE Imagination at work GE Heqlthcare We Fn Fostt Farameters Rasults Lisnlay LegEhnd k Lnscale w Innlhar tatus Ear w nuse Falleate Figure 216 Result
189. esults Menu Higure 61 3 5 7 Sample Results View Results ahsrat 13 12 96 14 25 Mwyr Horizorntal grlical abs BZEHLI ABs 28HL Absorbance ratio HITBTEHICB Sampls 1 Figure 82 Multi Wavelength Quantification Fraction Analysis GE imagination at work GE Heqlthcare Thiethods Edit VIEW Run Wid New Method A Clear Cascade Open Method B Delete Graph Tile Tefine Ilethnd Airanee Icons View A View Results Export Setup Prmt Print Preview Print 5 Hisgure 83 3 5 8 Overlay View mm Overlaw GLP Test Wi1 i ave erThiTm Figure 39 Typical Overlay View Wavescan Fisure 84
190. etais Run Options Wawelhgths Include all wawelenegths Add Wavelength Waweleneth gL ave enegthe gal Edit WWawglength Wawglengthd Rmmnwe WawglEhgth HH Euatinn Edit Eaulatinn Rimnwe Enuatinn Enuatinrn List Figure 171 Wavelength Bquation List 2 Wavelength 1 Add Wavelength Wavelength Title Bquation List GE Imagination at work GE Healthcare Wavrelength x Title Lambds Myawalan kh 5n Carncel Higure 172 Include all wavelength ON Edit Wavelength
191. ethod OK GE Imagination at work GE Heqlthcare Cuhure FE F IassayCUN ES LO Methad CUM woE Figure 220 Last 11 5 1 Dialog Box OK Dialng HIIHI Dr gna ssa 2 1 TN 1 None 3 ODL FDO ILIHH Dh Enal ssas II 1 HUHI maa NN a ne 1 rl lw et Factor ag Figure 221 Dioalog OK GE Imagination at work GE Healthcare Lna Cell ILhamingr Celll Brath rginal ssap Lell 2 Brcth A 5 l 1 Brcth pit 2 Cell 4 Eoh Cell5 Erol CellE Emphn Cell 7
192. g UL Hroth iH T Pal mt 1DOD Brnth IEpii 1 1 OCOD Brath Eplit 2 gt ctnr _ OK anca Figure 225 Split Split Split Factor 11 5 3 Close Pin Fost Pin mw gthnd tf Split Cnse Fisure 226 Close Close GE imagination at work
193. gth Wavelength At 511 7 8 9 Constant 0 302 10 11 Wavelength Wavelensth At 720 12 2 13 14 Constant 100 15 OK 16 17 Save 8 2 2 Details Page Eauatinns Rum ptins Tile heralni Lamrehl Filename IFi Temp Figure 175 Title Operator Comments GE imagination at work GE Healthcare Filename Date Temperature 8 2 3 Run Option Page
194. gth Fracton Analysis Add Remove Configure Print Preview Reaction Kinetics Michaelis Menten J Index Options GE imagination at work GE Healthcare Group Report 1 Overlay Header
195. gue box 8 5 1 Use with Sipper 8 6 Edit Clear Delete Del 8 7 Mult Wavelength View O
196. gure 54 Run Default Standard Method 3 3 6 Calculator Tm 2 Multi Wavelength Tm 3 3 7 Post Run i SWIFT T Wavescan HOLMIUM FILTER 09 10 9 File ethods Edt View Fn PostRun Window Help Mathematics FE Fgak Fin Pe re Lariwatie Tracg Figure 5 Post Run Soothing step interval Mathematcs
197. h EUCOSE OXIdaSE TN Elucose oxidase 1 Es ii pe 1 Graph glucose nxidase 1 raph elucose oxidase 2 2 Graph glucose oxidase 2 v3 Graph glucose oxidase 3 We 4 Graph elucose oxidase 4 NLOEEDD cese Bxidase Sdex GOXAD1 RKD Figure 57 Cascade Arrange Icons View 3 3 9 Help Using Help Index About 3 3 10 Run time Menu Abort GE Imagination at work GE Healthcare Display Hide Show Run display Show Hide
198. iew Michaelis Menten 7 Reaction Kinetics Post Run Options 7 1 Slope Reaction Kinetics 7 2 Mathematics 5 7 3 Derivative 2 7 4 Trace 5 8Michaelis Menten View Option Michaelis Menten 5 8 1 Michaelis Menten View 5 8 2 Viewing results 5 8 3 Overlaying results 5 9 Window 5 10 Help 6 TIMB DRIVE 6 1 6 2 Time Drive Parameters GE imagination at work GE Healthcare 6 2 1 Parameters Page 6 2 2 Details Page 6 2 3 Display Page 6 2 4 Run Option Page 6 3 Time Drive Display Options 6 4 Time Drive Data 6 5 Time Drive Run 6 5 1 Single Sample Mode 6 5 2 Use with Sipper 6 6 Time Drive Post Run Options 7 Quantification 7 1 7 2 Quantification Parameters 7 2 1 Standards Pa
199. iew gt Display View gt Graph View gt Results View gt Graph Standards Samples iraph ptions Display ntinns ft ly Show Standard Replicates im hone ly Show Standard gan 5nld ly Shaw Sample Rehlicates Fnss ly Showm Sample hean Ticks Peset Fisure 158 View gt Results ON Resu It ntinng Ip Tabulate Results ly hnwm Replicate Rgsults ly Shom Assay Results lg Show Standard Error 5haw Date Figure 1599 7 4 Quantification Data Standard GE imagination at work GE Healthcare
200. ine Ln eas hiathematics kE Fe Find k Fe rea k Leriwatiwe Trace Figure 102 Post Run Menu 4 2 Wavescan Parameters 4 Parameter Sheet New 4 Parameter lock 4 2 1 Parameters Page GE Imagination at work GE Healthcare aramEtErs Parameters hietails Display Run Options Wawleng1 hethod start Gtandard End hnnn 2 1 Time atep 1 nm Filename Data oan Rieterernce GCah Speed Temperature Mane
201. k for Bxternal Pelier Instrumentation GE imagination at work GE Healthcare OK Liemnrslralinn Library ltrnshec gHHH HItrngnec HH nrn ltrnsnec LDL HItrnshec 3LIHH nrn ltrnsnec ON pro tospec LN pro trospec ND nrn FTE trospec 4 ltrospec HH pro HItrnsnec 3 nrn FTE Fisure 17 sgtun Detfault HE TTIES Registratinn Cnmmunicatinns Spreadsheet Lanegluaee LF hata Loegegine Direct tn Excel Tn texxt filg OE Fisure 18 Spreadsheet Direct to Excel PC Microsoft Excel Excel To text fle Spacing Type
202. lit Split Title Original Assay Split 1 2 0 11 2 4 Run Option Post Run Se GE Imagination at work GE Heqlthcare Farameters Faraimetgrs Letals Fnst Run Lishlay Snlit Details Pun Uptions BE Lnck ample Harndling ho nf samples tn be B Included per cell 9 le nyeray Graphs charnger ly hn Results Figure 212 View Sample Handling 2 11 3 Post Run Display Parameters 3 Farameters Details Fost Rn Display Split Letai Run Options Im TI x Fnrmat D as None hin nlid Prnss Ob Ticks m Log DD Innn1 Reset Time x II GE Imagination at work GE H
203. matics Peak Find Peak Area Derivatives 3 GE Imagination at work GE Healthcare Reaction Kinetics 5 1 Reaction Kinetics 1 LDH 8
204. ml F Figure 10 Instrument Control StatusBar 4 data logging printer status GLP printer status Hile View Command Data Log Help Instrument Control Set up Command Section 3 3 Command Menu Command Connect GE imagination at work GE Healthcare EE Figure 11 Instrument Control Instrument Control oo coo ahznrhmnre ln ab LCnr l Facio Lm H ga Figure 12 Instrument Control Panel
205. ng Help Index About GE imagination at work GE Heqlthcare GE imagination at work GE Healthcare 6 TIME DRIVE 1 Time Drive 100 Common Menu Descriptons 3 3 Post Run Menu Figure 134 Post Run 6 2 Time Drive Parameters 4 Parameter sheet
206. ork GE Heqlthcare PPT can WFT Instrument nntrnl a File Miew Gammand ataLaug Help a Cannect Ll Cohnhect Discohnhect the instrument Coml Figure 24 Command gt Connect PC Instrument Control 2 2 2 Setting up an accessory Command Check Accessory TFT I Imstrument Contral File Wiew Command Data Log Help er w nnhert awglength Da 1 Mbs ln ams Check ccessnry CntiEure Check current instrumernt accessory Caml Figure 25 GE imagination at work GE Heqlthcare OK Command Configure
207. ptions Parameters Display horizontal vertcal Results GE imagination at work GE Healthcare Shomabs Readings le 5hamuEnuaknr ly ShombDate se ShamTms Figure 182 Multi Wavelength Display Options Toolbar Status Bar 8 8 Calculator Tm Tm Calculator Tm Mr hmnlyul dL Rl TAAAACGACGGCCAGT_ a ee 5 i E5m lt a a En mal 2 Clear Cielete FNA DN LlnsB Figure HH Im alcmlator em Figure 183 Tm Tm Tm RNA ssDNA T U
208. r Line style Font Size ONI 0 Display Print GE Imagination at work GE Healthcare 3 4 3 GLP GLP GLP Enabled 10 GLP GLP 5tar regults with data 5tare results with audit ral Fieure 62 GLP 3 4 4 Audit Trail Audit Trail LOG
209. rameters and Details Paee 7 2 2 Standards Concentrations Page 7 2 3 Run Options Page 7 3 Quantification Display Optons 7 4 Quantification Data 7 5 Quantification Run 7 5 1 Run gt Standards 7 5 2 Run gt Samples 7 5 3 Use with Sipper 7 6 Quantification Post Run Options 8 Multi Wavelength 8 1 8 2 Multi Wavelength Parameters 8 2 1 Bquations Page 8 2 2 Details Page 8 2 3 Run Options Page 8 3 File 8 4 Method GE imagination at work GE Healthcare 8 5 Multi Wavelength Run 8 5 1 Use with Sipper 8 6 Edit 8 7 Mult Wavelength View Options 8 8 Calculator 8 9 Multi Wavelength Post Run Options 8 10 Window 8 11 Help 9 FRACTION ANALYSIS 9 1 9 2 Fraction Analysis Parameters 9 2 1 Parameters
210. rk GE Healthcare Reaction Kinetics Auto Region Kinetcs Assay 2 Define Region 2 2 Display Results Slope Result Quality Full results Slope Hesults Tabulated EE INg TillE Slope 1 qlucose oxidase 1 91 6b7 lucoss niHdmsFE bh 4 qlucose nidaSE hh qlucose Oxidase 4d
211. sh Blank Display Busy Ret 1 GE imagination at work GE Healthcare 2 3 4 5 settle tme 6 7 Waste 2 3 Command
212. to temp region Define temp region Default Setting Defaults Setting 10 2 5 Tm File Tm Open Close Save SaveAs Method File Data File Standard File Spreadsheet File Export Set up 3 4 Print _ Group Report Data Report Spreadsheet Report Set up gt Print Opions
213. ual 4 2 GE imagination at work GE Healthcare 2 ANN 1 7 2 Quantification Parameters Standards Samples 2 Samples Standards Factor Standard Parameters RunzStandards Standard Curve Run gt Samples Sample Parameters Standard 3 Parameter Sheet 0 0 9
Download Pdf Manuals
Related Search