IO テスト FITC 標識抗体 CD7 IOT7
Contents
1. IO CD7 CCRF CEM 1 JURKAT 2 80 CD7 KM3 3 0 5 1 CCRF CEM T cell Leukemia 2 JURKAT T cell Leukemia 3 KM3 Non Non B Leukemia PBS 4 CD7 CCRF CEM JURKAT 32. 22 8 C 12 IO FITC CD7 IOT7 A07755 100 2mL wm oo 1 Reinherz EL et al ed 1986 Leukocyte a Typing I Vol 1 Springer Verlag pp 303 313 2 McMichael AJ ed 1987 Leukocyte a Typing ME Oxford University Press pp 144 147 210 214 234 238 239 241 480 484 579 581 889 895 3 Knapp W et al ed 1989 Leukocyte a Typing IV Oxford University Press pp 36 38 274 277 295 342 343 4 Schlossman SF et al ed 1995 Leukocyte a Typing V Oxford University Press pp 507 509 515 518 790 794 795 798 1503 1504 5 Beverly PCL and Callard RE 1981 Eur J Immunol 11 329 334 6 Bryan CF Newman JT et al 1987 Trans Proc 19 4340 4344 7 Clark P Normansell DE et al 1987 Blood 67 1600 1606 8 Clevers H Dunlap S and Terhost C 1988 Eur J Immunol 18 705 710 9 Denning SM et al 1988 J Immunol 141 2980 2985 10 Freedman AS and Nadler LM 1987 Semin Oncol 14 193 212 11 F
3. PBS 2mL 400 xg 3 6 7 0 5 1mL 0 19 0 5 PBS 10 24 3 Ficoll Paque 1 3 4mL PBS 2 Ficoll Paque 4mL 1 3 2 8 C 400 x g 30 4 Ficoll Paque
4. 10 1 20 L 1 PBS PBS 6603369 1 500mL pH 7 2 0 2 BSA 0 5 0 19 2 PBS VersaLyse PBS 1mL IOTest 3
5. 2 29 EPICS XL VS FITC Lumphocutes Leukocytes 88 69 608 BB1989 Count 48 SS 128X128 28 299 488 1 1 18 199 1808 1 1 18 19 1999 FITC FITC CYTO TROL 6604248 Fc
6. IOTest CD45 FITC CD14 PE A07738 Fc IOTest T B
7. 10 3 5 5 1 0 1
8. 3 6 4 5 Ficol Paque 8 9
9. 5 PBS 2 8 C 400 x g 8 6 PBS 7 2 8 400 xg 4 8 9 10 100 2mL Oo TR PBS 2 lt 8 400 x g 3 10 PBS 5x103 mm 5x 106 mL 11 90 1 2 1 12mm x 75mm 2 Ficoll Paque 5x10 5 x10 mL 100wL 3 20 L
10. A07800 12 5L 0 19 PBS VersaLyse PBS 1mL IOTest 3 A07800 67 5L 0 5 PBS 3 A09777 VersaLyse IM1400 OptiLyse B A11895 OptiLyse C A07799 IOTest 3 4 IOTest Mouse IgG2a FITC A12689 5 Ficoll Paque Phamacia 17 0840 03 2 1 100 L 5x103 mm3 10 x 10 mm
11. CD7 40kD CD7 Pan T T T B NK AML CD7 CD7 8H8 1 IgG2a FITC Fluorescein isothyocyanate 485 509nm 504 541nm CD7 CD7 FITC 1 IO 2 EDTA
12. PBS 3x 103 mm3 1 25 C 500 xg 5 2 3 4 DxH800 5 PBS 5 10 x 103 mm3 1 12mm x 75mm 100L 3 20 L IOTest Mouse lgG2a FITC 20L 15
13. 2012 11 1 20200EZY00112000 A07755 Wa COULTER I TT IO FITC CD7 IOT7 1 2 3 4 IO FITC CD7 IOT7 FITC IgG IO FITC FITC Fluorescein isothyocyanate 1 100L
14. IOTest Mouse lgG2a FITC 20L 4 2 8 30 5 PBS 2mL 2 8 C 400 x g 5 6 5 7 05c1nD 0 5 PBS 8 24 FS 90 SS FITC Log
15. nology Today 8 308 315 135 0063 5 7 TOC TEL 0120 566 730 FAX 03 5530 2460 135 0063 5 7 TOC A07755_1211
16. oon KA and Todd RF 1986 Blood 68 1 31 12 Hannet l et al 1992 Immunology Today 13 215 218 13 Haziot A et al 1993 J Immunol 150 5556 5565 14 Keizer G et al 1985 Eur J Immunol 15 1142 15 Kishimoto TK et al 1990 Proc Natl Acad Sci USA 87 2244 2248 16 Koepke JA and Landay AL 1989 Clin Immunol Immunopathol 52 19 27 17 Kung P Goldstein G et al 1979 Science 206 347 349 18 Laffon A et al 1991 J Clin Invest 88 546 552 19 Lanier LL Le AM et al 1983 J Immunol 131 1789 1796 20 Lum LG 1987 Blood 69 369 380 21 Maissen B Rebai N et al 1982 Eur J Immunol 12 739 747 22 Nadler LM Anderson KC et al 1983 J Immunol 131 244 250 23 Polk B Fox R et al 1987 New Engl J Med 316 61 66 24 Rebai N Maissen B et al 1982 Eur J Immunol 13 106 111 25 Reinherz EL and Schlossman SF 1980 Cell 19 821 827 26 Reynolds CW and Ortaldo JR 1987 Immunology Today 8 172 174 27 Rouas Freiss N et al 1997 Proc Natl Acad Sci USA 94 5249 5254 28 Spits et al 1985 Eur J Immunol 15 88 91 29 Tedder TF et al 1985 J Immunol 135 973 979 30 Tucker GC Aoyama H et al 1984 Cell Diff 14 223 230 31 van Agthoven A et al 1991 Eur J Immunol 1981 11 18 32 van de Velde et al 1991 Nature 351 662 665 33 Warren SM et al 1991 Immunology 72 150 157 34 Wrnke RA et al 1983 New Engl J Med 309 1275 1281 35 Zola H 1987 Immu
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