（計 画 書）
Contents
1. uduuggudduddudU uu nooo JUDO MU o mo JU OUO QUO UUUUD 0 000 0000000 Dn U o D 00 DO r oo ii moooo 49 55 HH UL UL UL UL UL UL UL B 31000000000 Peg100000000000000 000000000000 000000 PEREGO EUSAGOOOOOO 00000000 000000000000 PREQOOOOOOO 0 EUSAD DUO OOOO DO DO DO 3 20 000000000 DOBO DD TO GT DDD OL DU ss LI u u u u UN OUGUO0UOUL 0000000000 32 L2. U PBJ 3000000000000000 00 Ps0 00000000000000000000000000000000 OOO0O0Aoraxis DD UL UL Soo 000 2000 OE 400 00n20 10000000 000000 000000000 0000 15 30C 4 8 C TR PR a Y U YY 032 JUUUUDUDUDUUUHUU32 mm MM enn 0 0 n I u 0 0250 lug ul 0 0 0 mr BEN polak UUU 0 0 0 0 0 m 0 D UU D u 0 020ng 5 D 0 0 0 u 0 ae L soooo0 ppponome GE 0510 00000000 150 0 0 0 0 R 0 872 en n D 1000 000000001010 Barbara Hughes A Manager 0 D 170 ell 30 DUBBI pd d ul 410000000 pod00000000 170 00000000 UUUDDUUUUUUUDUUUUUUD 041 wo l zu 20 ng 20 oo oo un loo lan Ian UL 00000000000000 0000000 lol 0000000000000 E JU 000000000 ol 00000000 J Toi 00000000 NE zl
3. 0000000000 00000000 o 0000000 0000000 mmm hr 00000000 01 o T O boo o 420000000 O00000000000 4200000000 0420000000 UO UO CDL LU U UL LLED O HB PED E UOZUUOUUNUUNUUWUUUUUEC EL UWUUTUUUOUUOUUOUUUUUWUU CI 00000U00000 3000000 UUTUUTUUOOUULUUUC U 900000 JUIUUUUDDUUUDNUUDUDUOL 900000 Hum E H L E EN EA il ELISA KAB ER KEE ELSE EU O00000000 000000 000 CDL LU U 20000 UUTUUTUUOOUUUU LT 4300000000000000 UANONOOUUEWSAJOUUUUUUU 0004040 EUSATUUUUUDDD 4 B B CELO DL OUUUU EUSAR o D DL DO ODD OO D D BB D DI OH SAT D DL OL I Uu D D D D DE OE DE UE DO DO D D U U OH OL OO OD D 0D O HO 7D D D CE DE OO 7D D 7E CT 7T UI L U 2 EU SAL EDT UE DU L OEUSADUUUUUUIUUUUUUUUUDDDDUUUUUUUUUUUUUUDDDOD
4. OO00000000000000000000000000000000 442000000 UL B5 GO TO TTT UWUUOUUUOUUWUUOUUOUUUUCUUL 0 4420000000 x S 00000000 au O Lug L El EI EE O O O O PBLE 47 LED EE E EE A O 10 50 10 50 ny L U00000 DOB ODO qti GT TO ih TO TTT B TT EE E E E EE Ecco cc Du dI EEE EEE EEE HEH GE puugamuauBimiumPREA40mnagmiiubBbaaguguuuttuttutuuuuudtult UUOOZUONWEUSANOUONOOOOONOUUOOOUOUONOOUUUUOOIUOU EI EDIB TUE PEWNE ph h BEBB LE BD 13 D E pe E de E E E aaa E E EEE D L il li HO OOO DDD 0000 TT don HE BEE B UUTUUOUUUUUL OT TT 5H OO Uti H DS JO E a au hui Eee REESE CEE i IL Bu h Pht atera ea JEMI dE EN ET UUUUDUUIUUUUs2UUUDUDDUUDUUUuuuuuuUuuuUuuUuuUuUDDnoDnno UDU320IUUUDDUUuuuuuuUuuUDDUUnuguuUuEUSADDUDDUUUUUUDDOD EE
5. PBDE Congener 209 370 3000 2 2 4 5 Tetrabromabiphenyl 5 6 250 22 44 55 Hexabromobiphenyl 130 gt 1000 2 4 5 Tribromobiphenyl 160 2600 2 2 5 Tribromobiphenyl 175 2900 24 5 Tribramabiphenyl 240 3200 2 2 ARD Pentabromobiphenyl 320 gt 1000 3 3 4 4 5 9 Hexabromobiphenyl 1000 21000 2 24 46 0 Hexabromabiphenyl gt 1000 21000 Decabromabiphenyl gt 1000 gt 1000 PCB Aroclor 1254 3 180 PCB Congener 37 160 2000 PCB Congener 77 1000 1000 2 2 4 4 Tetrachlorobiphenyl 16 350 2 2 4 4 5 Pentachlorabipheny 5d 1500 PCP 3300 210 000 2 4 D 210 000 gt 10 000 The following compounds demonstrated no reactivity in the PBDE RaPID Assay at concentrations up to 10 000 ppb Biphenyl 2 5 Dichlorophenal 2 3 5 Trichlorophenol Di n octyl phthalate 17 Ze PBDE 3 2 0 FRI 7k OS O HIN E SERA BAR 4 RY Jr SAM UI Z lt x PBDE Hn ppt ppi 7818822 ppt FIRE 96 8625 54 _ 287 _ 18 mA PBIE T ANA Bioanalytical Approaches for POPs Detection Development of a sensitive magnetic particle immunoassay for polybrominated diphenyl ethers Fernando Rubio Carmen D Parrotta Qing X Li Weilin L Shelver Fernando Rubio 1Abraxis Lic 2Department of Molecular Biosciences and Bioengineering University of Hawaii at Manoa 3USDA ARS Biosciences Research Laboratory Introduction Polybrominated dipheny
6. DEED E Og EERE EN ENE Ne Ea DEE rE SEVERO SE EE FED 14 CE FE hii SERE REESE O O AV CE A gaga O OO GU E SV ET E EE EEE 0l TT TG TTT TT TTT IT ED T EDI EEE ANA AO A SA E HEE EE E E EE E EE DDE REE UUUL Devel opnent of a Sensi ti ve Magneti c Parti cl e I nmunoassay for Pol ybronnnated O phenyl Ethers Paper presented at the 2005 D oxi n Conference hel d in Toronto Canada on 8 22 05 O O PBLE 4 U DL ODO TTT u UUIUUUUDDDOUUDDUUDUUU 15 HOU HSH PBDE 1 LD Bien Absorbance 450 nm 1 30 1 09 0 392 u 71 0 38 0 28 25 5D 150 500 1000 PBDE Congener 47 pgimL 0 025 1 L 16 Mellt PBDE 2 1 00 AED FE B B 905 4 5 BH Lei F E E CELH PR R BO EZ ESO LEE ERE REGO 2 i C EL T R LDD50 B Ba Compound ppb ppb PBDE Congener 47 0 017 04135 PEDE Congener 93 0 02 0 15 PBDE Congener 28 0 045 03 PEDE Congener 100 0055 55 PBDE Congener 153 0 075 10 PBDE Congener 154 3 5 580 PBDE Congener 183 13 5 2000
7. 000 OEUSAO TO ODO DO DO TT DDD DD DO 0000000000000 JUIHIDUIUTIUDUUDDUHIDUUIUUDUDUUDUUUUDDUDWU EISA O LO DD UL UL E 43 pm E E RUN ED EL DH US Es Bn EE Lot No KG DR AR 1 ET Pr E OD LEED OU HOON o 115 I D e T D E EE EE Lot Nb aaa aa 1 EJ al ET EJ MIN d 0000 D 12115 DU UL 440000000 UUOUUOUUUWOUUOUUOOUUNUUOOUUNUUOOUUE U ELISA 000000000 UD dl EL EE BLE HE EEF BEEN E Q00000000000000000Mde 6000000000000000000q00O FF CHEER EE EE ERE bo HDH EFE UUUUUUUD UUNOUUUNOOUUUNOUUUNUOBKIOUUUONUUUONUUUONUUUELI 000000000000000000 paraneter logistic fitting 0000000000 I U U GENS SL T UUBDOBIBEE Ga a Eee 10 00000000000 0000000000000000000000000000000000000a0000 00000000000000000000000000000C00
8. 08 8 8808 8 88 8 eee DO QO DOOD n n n Hee eee DO OD FFF n mnm mmm mmm e I nana rara nia DO OOD eee DO OD ect m mmm mm n narra narra rara DO QOD DOD ree DO 0 2000000000 cer ee DO on DD DO on DD DO OWOOUUWWUOU eeeeeeeeemmmmmmemmemm mmm m narra arara nara renan DO N9000000000 eeeeeeesessrsrsssrssssrrrerrrsrsrrrrerrrrrenrn DO DODO e DO i eri HB ed PE E A aa aa LE LA D WEN ENER Z SSA ACA ACC ELISA OA O EE A UpDgugiuiibut lut Q 10000000000 TT TT TTT TTT TTT TT TO TTT TO TUDO JUIUNUIUDUUDDUUUUUUUNUUUUUDOUUUUUDUUDUUUDDOUUUUDDDUUu EE pd a E a a nau nn aan an nn nan aaa AO hii an a ila EEE UUTUUTOUUUWOUUOOUUOUUOUUOUUUUNUUUOOUUNUUCOUUEC CI EAH EEEE DO A EE EDE EDE EE P P hhi iB E LLEF AAA O aH TERR HE EE H EEE UUTUUTOUUUOUOUUOOUUWOUUOUUOUUUUUU ULL TOT GT OT JUIUUIUUUDUUUUUUUDNUUUUUDOUUUUUDUOUOUUUDDOUUUUDDUUu IO ELSE Eee EEF ET CE EE TC TO GT TO TTT TT RRE SO BUER ERR EE HE EL HER EI EIEH EE EE 120000000 DOBO OO DD OD D
9. 0d o 0000000000 UUUUUUUUUUUUUUN PEW OOOOUU00 0 PB Standard Solution PBDEA47 DADO ODO 59 n O UO TTT OD DO E qap D D 000000000000000000000000000441G0000000 0441 0000000000 0G0000U0 1000 M 00000000 weu PR BE 47 00 ON IO epu OUUUUU e nr u an O 0 025 0 05 0 10 0 5 1 0 n Bier PBLE Congener 99 0 025 0 05 0 10 0 5 1 0 GU I ULL PBLE Congener 28 0 05 0 10 0 20 1 0 5 0 PBLE Congener 100 0 10 0 5 5 0 10 50 000UU0 L 3 1 BO DDD G ODE 01000 404000 EL G lh EEE TT TO TT TO ILU e e BH E 00000000000 A EE IH FH DN E BS S FEIN HEAR HEEE Ca ub 00000 Sp O 35DNU 1DUUUUUUUUUUUUUUUUUUUUUUUUUUUU N DEE i 0000000 10000 0000000000000 0006 0000 5 00000000 10 35D0000000 000000060 KARELA EEE i h b DEE E 00000 O0 11 I tii 30000 8000000000 800000000 JUIUUUIUDDUUDUUDUDUUU O00000ggn80000000000000000UO UUUUUUO 000000 1000000 3000000000000 0 DD 0000000000000 UUUDUIUUUUDDUDUUUUUUUUDDDUUUUUUUUDDUUuuUuuUascnD LED
10. O DD AEE B Om h Lt o UUUUUUUL o DUO DOO DO DD OD DOLO O IL E a ea UUTUUTUUOUUUNUUOOUUCOUUCI 2100000000 DOBO DO DO B UL 021 DO000000 U 5168 457 0841 OUO OUUU 052 692 8481 052 692 8483 koj na agoyakankaken of f i ce to M 105 0023000 00000002010 HOOUUUOUO et 0000006 OOO UU UU 03 5444 9891 03 5444 9860 eco j echemco jp 220000 UUTUUTOUUOUUUUNUUOUUUCUUU LE LIEU ERE E EE E EA E tl 000000000 000000 000000000000005 UUUUUUUUUUDU 22 0 23000000000000 ULL E UD H 23 0 Uni UUUUUUUUUUUUU EISADO OOO UUUUUUUL ELI SA UU UO UUUUUUUD SADU UL 0000000000000000 Ol UUUUUUUUUUUUUUU 000000000000000
11. U 46 U UU ULBDLULLUULL JO L DL EEE UUUUUUUUUUUUUUU i U D D DI 8 O PB EU SA lUIUUHUDIUDDUUUUUNUUUULN d U Uu Uu Er UUTUUTUUOCHOUUOOUUNUUOUUOOUNU HOOUDLUOLDILOLU DLL UUWOUUNUUU UCCTT GC b lin b iY TGR TOTO TODO EE NDS EE dt ELE E EE ph Th CL m h RTH EB ED UWUUUUL UWUDTUUUUUUCI UU LL U J EE E EE ui LL EEE Ud LL DDDDDDDDDDD ce mmmmmmmm mm mm t MIHI LIDO DO DOO OOO 1 120000000 1 ann nn a OOOO na 1 UO ce eee eens 1 220000 reee 1 2300000 eese n n n A HH 1 S RRE O AKIRA SAKE Er Na E eee ene 1 8 88 NEE OR 1 320000000000 DOOD eeeeeeseesieeesemenmmmmm m m m e HIR HH 1 4 1D0DDDDDD FFF eee 1 420000000 mmm m mme 1 4agu dudgugugggmnmnmnmnHleseeeeeeseeeseseeiemmmmeHmHeIA I R 1 AAT 8 885 8 8 8 GU DO DIDO OOD eee DO OOO e E DO on 8
12. d for the screening and quantitative analysis of PBDEs is required Rapid sensitive accurate and cost effective enzyme immunoassays ELISAs have provided the analytical chemist an alternative too to traditional instrumentation methods Magnetic particle based ELISAs have previously been described and widely applied to the detection of pesticides and other environmental contaminants in various sample matrices including water soil produce and fish tissue The uniform dispersion of the particles throughout the reaction mixture allows for rapid reaction kinetics precise addition of antibody and superior analytical sensitivity This paper describes the assay performance of a PBDEs magnetic particle based ELISA in ground water Materials and Methods Carboxy terminated superparamagnetic particles of approximately 1 mm diameter were obtained from Seradyn Indianapolis IN N hydroxysuccinimide NHS and 1 ethyl 3 3 dimethylamino propyl carbodiimide EDAC were purchased from Sigma Aldrich St Louis MO Rabbit anti PBDEs serum 122 was produced by immunizing the rabbit with 442 4 dibromo 5 2 4 dibromophenoxy phenoxy butyrate BSA in according to Shelver et al The PBDE ligand and horse radish peroxidase HRP were conjugated via NHS and EDAC reaction to yield PBDEs HRP conjugate Abraxis Warminster PA TMB peroxidase substrate was purchased from BioFx Randallstown MD PBDEs PCBs PCP and 2 4 D were obtained either from Chem Serv
13. ed EE UUUUUDUL UUUDDUUUUUUUUUUDDDUDUUUUUuuuiUUUUUDDDDUUonn E PPP an an an HE nn nn in a a naan a aa i a DI D EEE L 30000000000000000000 10000 200000000000 00000 U TTT TTT TOT TTT TTT UUTUUTUUUOOUUOUUWOUUOUUCOUUUEC U o 0000000 D D D D PBE Congener 99 PELE Congener 28 PBLE UU UU PBLE 4 congener 100 I UU 00 OO DD 0 0000000000000 30000000000000 DECIDE a A EET EE ED DOI ED e BIS ETE DEUS UFBE400 590000 00000 59900000 x1001ji000000000000080 UUDUDUUOOTOOOOOOOONOOONONOOOUUUUUU SI I I UU UI UUDUNOONOSAOUUNOOONOOUUUUONOOOOOOUUOUNOONIIUU UUUUDUIUDUDUUUUUUUUUUUUUUDDDDUUUUUAUUDUdiO UDUDD DUU 12 CE E E n a ee Ee essee eeaeee Ene Geete EEs Seet e EN e EE o 00000 I 1 2700 00000000000000000000 TTTTIT DO DO U PRE AZ IOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOU
14. ice West Chester PA or AccuStandard New Haven CT The anti PBDEs coupled magnetic particles were prepared by NHS EDAC activation according to the procedure supplied by Seradyn The unbound NHS EDAC was removed from the particles by magnetic separation and washing two times with 50 mM of 2 N morpholino ethane sulfonic acid MES buffer pH 6 0 The PBDE antiserum and the activated particles were incubated overnight at room temperature with agitation The reaction was then guenched with glycine buffer and the covalently coupled anti PBDEs particles were washed and diluted with a Tris saline BSA 905 19
15. l ether PBDE mixtures are manufactured as flame retardant additives for electronic equipment plastics and textiles Three types of commercial PBDE mixtures find wide application namely pentaBDEs octaBDEs and decaBDEs The penta formulation consists of a mixture of PEDE congeners that includes BDE 47 BDE 99 BDE 100 BDE 153 and BDE 154 The octa formulation consists primarily of BDE 183 while the deca formulation consists primarily of BDE 209 North Amenca accounts for approximately 98 of the global demand for the penta formulation PBDEs are ubiquitous environmental contaminants their bioaccumulation has led to the detection of PBDEs in many species of wildlife human blood plasma and in human mother s milk PBDEs are structurally similar to polychlorinated biphenyls PCBs polychlorinated dibenzodioxins PCDDs and thyroid hormones and therefore may interfere with thyroid hormone homeostasis Because of their potential health consequences it is desirable to have a rapid and high throughput assay to monitor PBDEs The quantification of PBDE samples is usually done by gas chromatography mass spectrometry GC MS or GC high resolution MS GC HRMS While these methods are reliable they are sophisticated and require extensive purification using large volumes of extraction solvents The analysis of large numbers of samples using these techniques is not feasible Therefore a rapid simple and cost effective metho
Download Pdf Manuals
Related Search