研究成果報告書 - KAKEN - 科学研究費助成事業データベース
Contents
1. FDEIA IgE w 5 2 IgE2. 5 2 IgE 2 3 A
3. NCBI Triticum EST PloteinPilot mm
4. 5 IgE FDEIA 8 ELISA exercise induced S Hy
5. 6 DNA 2 Rosetta DE3 IPTG 3 CAP FEIA IgE CAP FEIA IgE FDEIA 48 16 AD 12
6. 124 20 2012 Clin Exp Allergy 2012 4 7 22 1 IgE 2012 a 42 42 7 13 15 A 4 a 13 15 Ia
7. B IgE 1 native immunoblot Native 70 100 kDa 4 CBB Fig 1 native IgE FDEIA 2 2 inmmunoblotting native IgE Fig 1 I Patient Control BB Pl P2 CI C2 3 2 1 2 1 2 1 1 2 kDs 113 61 47 8 Fig 1 IgE immunoblot analysis with recomhitnant HMW gluteni
8. 16 7 92 99 w 5 93 8 92 9 509 I o 5 1 IgE FDEIA C1inical and Experimenta1 Allergy Clin Exp Allergy 2012 Augi42 8 1293 8 doi 10 1111 j 1365 2222 2012 04039 x 5
9. nano LC nanoLC gq 4 MALDI TOF TOE 5800 ABsciex MALDI TOF TOF MSMS MASCOT ProteinPilot ABsciex B CAP FEIA 1 1Ax2 pEWHx pEWHx inverse PCR N 6 DH5 a
10. native A 2 1 70 iTRAQ MALDI TOFTOF MSMS PloteinPilot EST
11. 1 2 1 6 0 S 2 2 70 3 iTRAQ 7 0 iTRAQ AB sciex C18
12. IgE IgE ow 5 IgE 48 FDEIA 43 8 w 5 81 3 16 7 AD 87 5 w 5 0 12 5 o 5 81 39 100
13. 2012 7 Shiwaku K 4 6 1 24 2012 5 11 13 TAKAHASHI HITOSHI 10432618
14. o 5 IgE FDEIA The aims of this study were to identify wheat proteins in sera of patient with wheat dependent exercise induced anaphylaxis WDEIA during provocation tests using 1TRAQ based analys1s and to evaluate usefulness of CAP fluorescent enzyme 1mmunoassay CAP FEIA using recombinant high molecular weight glutein In 1iTRAQ based analysis although wheat proteins were detected inpatients sera those wheat proteins were not nvolving with allergic reactions When recombinant HMW glutenin was immobilized to CAP FEIA it was confirmed that detection of specific IgE anaginst recomb1nant HMW glutenin is useful for diagnos1is of WDEIA when combined with the CAP FEIA recombinant omega 5 gliadin test 2010 1 200 000 360 000 1 560 000 2011 900 000 270 000 1 170 000 2012 800 000 240 000 1 040 000
15. 2 900 000 870 000 3 770 000 Th Food dependent anaphylaxis FDEIA FDEIA E FDEIA 60 FDEIA 5 gliadin
16. C 19 K 25 6 7 15201 B 2010 2012 22790930 Analysis of wheat protein in sera from patients with wheat allergy using mass spectrometer TAKAHASHI HITOSHI 10432618 iTRAQ IgE
17. Kohno K Matsuo H Takahashi H Niihara H Chinuki Y Kaneko 5S Honjoh T Horikawa T Mihara S Morita E Serum gliadin monitoring extracts patients with false negati ve results in challenge tests for t he diagnosis of wheat dependent exe rcise induced anaphylaxis Allergo1 Int 2013 62 229 38 Chinuki Y Takahashi H Dekio 1 Kaneko S Tokuda R Nagao M Fu Jisawa T Morita E Higher allerge nicity of high molecular weight hyd rolysed wheat protein in cosmetics for percutaneous sensitization Con tact Dermatitis 2013 68 86 93 Chinuki Y Kaneko S Dekio I Ta kahashi H Tokuda R Nagao M Fu jisawa T Morita E CD203c expressi on based basophil activation test f or diagnosis of wheat dependent exe rcise induced anaphylaxis J Allerg y Clin Immuno1 2012 129 1404 6 Morita E Nabika T Chinuki Y Yamasaki M Takahashi H Prevalence of wheat allergy in Jap anese adults 1 101 5 Takahashi H Kohno K Matsuo H Tanaka A Allergol Int 2012 6 Chinuki Y Maruyama N Morita E Recombinant high molecul ar we1ight glutenin subun1t specific TgE detection is useful in identif ying wheat dependent exercise induc ed anaphylaxis complementary to rec ombinant omega 5 gliadin specific I gE test 2 1293 8 4
18. n snd native HMW utcnin IgE binding to recombinant HMW glutenin lane 1 and native HMW glutenin lane 2 was dctermined with immuroblot by using scra of WDEIA paticnts P1 snd P2 who had scrum IgE binding nredominantly to cpitope peptide of HMW glute nin As a negative control sera of healthy subjects werc used C1 and C2 2 native CAP FEIA native CAP FEIA FDEIA 10 AD IgE 4 FDEIA 16 12 native native
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