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GS2-2-3

1. 3 2 3 2 1 4 1 2 L GS2 2 3 2 Fig 6 pH Fig 6 Fig 7 Fig 5 pH DissolvedOxygen ave Saturation dissolved amount of oxygen Tempareture aVe 38 Sm 4 wn 37 nn oa 36 wn wn DissolvedOxygen mg L wn 35 0 2 4 6 8 1012 14161820222426 28 30 Time min Fig 6 within the PBS solution in which contains the fresh skeletal Time series dissolved oxygen concentration level muscle as the positive control study pH ave Theory pH Tempareture C ave 7 5 38 7 3 7 1 E 37 5 6 9 E 37 6 7 E 6 5 F 36 5 8 6 3 E 36 dE 35 5 5 7 MEAN SD n 7 5 5 ETT 35 0 2 4 6 8 1012 1416182022 24 26 28 30 Time
2. MM Fig 2 Thermostatic Bath Fig 2 Oxygen consumption measurement system GS2 2 3 1 3 3 v 3 1 pH
3. Fig 1 Desigsn proposal diagram of the system 37 C pH ee OO Dissolved oxygen amp pH meter 3 2 Fig 1 Tygon Tube Graduated Cylinder 4 SN AirSaturationO gt PBS ess 37 C an Fig 1 Il pH
4. 0 5 mgL pH 1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 Temperature C Fig 3 The Saturation O gt inside the distilled water as the faunction of Temperature which w as given by the sensor manual 1 PBS 37 C Fig 3 6 8S mg L 3 4 PBS Fis 4 pH
5. m 1H pH Y 0 0037X 0 3279X 13 i i 917 i pH S 4 Measured Data Approximation y 0 0037x210 3279x 18 917 Saturation of within the disilled water dissolved oxygen mg L LIFE2013 2013 9 2 4 pH ave Temperature ave log H pH Temperature C MEAN SD n 5 5 LuALMeniNein oo hat 0 2 4 6 8 1012141618202224262830 Time min Fig S E Time series of pH level and temperature inside the oxygen saturated PBS solution 3 5 0 2 mg L pH pH0 02 Hig 4 Fig pH 0 5 mg7L
6. pH 3 pH 3 1 AirSaturationO PBS 100 ml 37C 3 g 1 PBS 2 1 30 90
7. E Fig 5 DissolvedOxygen ave Temperature ave 7 5 37 0 7 3 36 5 36 0 amp 7 1 x 35 5 6 9 35 0 9 67 8 34 5 MEAN SD n 5 6 5 34 0 0 2 4 6 8 101214161820222426 28 30 Time min Fig 4 Time series of Dissolved Oxygen concentration level and temperature inside the oxygen saturated PBS solution TamnararinraT 1 Fig 4 Fig 5 0Olmin 6 min 37 C 1
8. t 2 6 df 8 p lt 0 05 GS2 2 3 3 Tempareture C LIFE2013 2013 9 2 4 PositiveControl NegativeControl 4 7 5 Saturation dissolved amount of oxygen 37 C 90 7 3 55 mg L 5 1 Navi pH Difference 3 55 mg L DissolvedOxygen mg L ww P182 P184 P185 2 5 2 EE P55 0 2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 3 1 Time min P199 Fig 9 Comparison of oxygen consumption level between the fresh and non fresh skeletal muscle which was indirectly measured through the measurement of the dissolved oxygen co
9. DF REE Ld C5 0 2 4 6 8 101214161820222426 28 30 Time min Fig 8 Time series pH level within the PBS solution in which contains the non fresh skeletal muscle 4 5 4 5 1 Fig 9 37 C 3 14 mg L 1 24 mg7L 3 55S mg L 2 3 14 mg L 1 24 mg7L 2
10. LIFE2013 2013 9 2 4 GS2 2 3 Development of experimental setup to measure the ssue oxygen consumption Sayo SAWADA Shibaura Institute of Technology Masahiro SHIBATA Shibaura Institute of Technology Nobuo WATANABE Shibaura Institute of Technology Abstract Tissue is consuming the oxygen at all times In other words the life activities are closely related to the oxygen However few conventional studies concerning the tissue oxygen consumption were performed The other sides several studies about the oxygen consumption at the molecular level were reported The diasgnostic method of the tissue viability is still under the research and that is leading to the criterion judgment for the joint surgery of amputated limb The evaluation of the tissue oxygen consumption is the possible its candidate We firstly developed an experimental system in which the tissue oxygen consumption level can be indirectly measured by the change in the dissolved oxygen concentration level The prehminary study using this system showed the different oxygen consumption level between the healthy and non healthy skeletal muscles which is suggesting the feasibility of our proposin
11. g method Key Words Tissue oxygen consumption Tissue viability 1 Water Flow gt Dissolved oxygen amp pH mete e Tygon Tube 1 Sealin OIL ILH 8 mc ee Hillll Mi 2 MI a 2 1 SI
12. min Fig 7 Time series pH level within the PBS solution in which contains the fresh skeletal muscle as the positiVe control study 3 2 2 4 1 24 pH Fig 8 Fig 9 Fig 8 Fig 7 pH 37 5 36 5 35 5 Temnareture C1 Tempareturel C1 LIFE2013 2013 9 2 4 DissolvedOxgen ave Saturation dissolved amount of oxygen 7 5 Temparreture 38 7 6 5 37 5 g 5 5 0 bn 5 Ld gt 36 5 6 4 5 5 2 SS 4 36 5 8 3 5 3 35 5 2 5 MEAN SD n 3 2 E ONPPPUNPPTPPHYWPPHIYUPYPPOEPYYIEPYYSPYPYPUUWDPI 11 0 2 4 6 8 101214161820222426 28 30 Time min Fig 7 Time series dissolved oxygen concentration level within the PBS solution in which contains the non fresh skeletal muscle pH ave Theory pH Tempareture ave 7 5 38 73 7 1 37 5 6 9 37 6 7 6 5 36 5 2 6 3 6 1 35 5 5 7 MEAN SD n 3
13. ncentration Fresh tissue Notfreshtissue Tissue oxygen consumption mg L MN TT TT OT th Fresh tissue Not fresh tissue Fig 10 Theresult of statistical significance test student t test P lt 0 05 4 5 2 PBS pH pH ATP pH Fis 11 pH pH PositiveControl NegativeControl Theory 7 5 FE 7 4 7 3 6 7 MEAN SD 6 5 ii mt ion Tinie bahia ie 0 2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 Time min Fig 11 C
14. omparison of pH value between the positive and negative controls GS2 2 3 4

GS2-2-3

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