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1. 2 CO 1 x 1 20 C 2 6 1 100 YSI 7701 1 20 13 1469 1976 2 Honma M et al Biochem Med 18 257 1977 3 Sato T et al Clin Chim Acta 110 215 1981 4 1983 5 36 998 1981 6 40 345 1985 7 26 435 1985 8 58 1080 1982 9 30 105 1982 10 61 912 1985 gt 103 0014 1 23 8 TEL 03 3668 8558 FAX 03 3668 8407 4 2 10 1 TEL 0479 22 00952. 87747 16100AMZ03212000 1 RI cAMP
3. 1 3 cAMP 0 1 4 1 RI 2
4. 6 04 C 2 10 100 500 800uL 4 C 3000rpm 3 1 EDTA EDTA 510mM 1mL EDTA2Na 1 9 3 7mg EDTA 500mM EDTA4Na mL 1020 EDTA 100uL 100 10 800 L 2 5mM EDTA 50 200 5mM EDTA EDTA 100 100uL 100uL 10
5. 800uL 3 AM 2 PH 2 1 2 No 1 2 2 No 3 4 2 No 5 6 16 No 7 22 nx2 No 23 2 No 1 lt 6 100 3 No 7 lt 22 lt m 100uL 4 No 23 100uL 5 251 cAMP 100uL 6 No 5 cAMP 100 7 No 1 lt 4 100uL 8 0 4 C 1248 9 No 1 2 B F 500uL 5 30
6. 10 No 1 2 500 500 11 No 1 2 3000rpm 4 C 5 500 12 5 1 B T 2 3 cAMP cAMP Uk Us 100uL isl cAMP 100uL B F 500uL 3000rpm 4 C 5
7. cAMP 1 1 EDTA EDTA 2 3 2 1 2 cAMP 1 1 Po A B6 9 1 2
8. 500uL 80 60 40 B T 20 1 10 100 cAMP pmo mL 1 cAMP Ellsworth Howard 2 cAMP 1 1 0pmol mL 80pmo 1 mL B T1 38 2 3 cAMP AMP 30 lt 60pmol mL 8 15pmol mL 1 5 3pmol mL 20 3 3 6 C V AMP 30 lt 60pmol mL 15
9. 1 1 A 1 11mL 2 B 1 1 2mL 3 1 20mL 4 cAMP 1 1mL 5 5I cAMP 59 2kBq 1 10mL AMP 2 251 6 cAMP 1 10mL AMP 7 1 28mL 8 EDTA 1 1mL AMP B F AMP 251 cAMP cAMP cAMP 25 cAMP cAMP
10. 20mL 80nL cAMP 201 1 2 3 2008 8 2 AMP 3 2 mL 1 1mL 1mL 4 B F 28mL 28mL 5 3 500uL 7 WI cAMP 100uL 1 1 100L 10 2 800uL 500 500uL W 80 lt 0 625pmol mL
11. 4 0 625 lt 80pmol mL 2 1 1 HIV HBV HCV 2 1 20 C 2 3 4 3 1 2
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