Nucleic Acid Isolation System QuickGene
Contents
1.2.
3.
4. LR All rights Reserved Copyright 2007 QuickGene 610L sei II EE i 0 P
5. 2 20kg 24kg 30cm 15cm 15cm AC100V 15 30 C 30 80 RH 24kg
6. I O LCD LED LCD LED 20 QuickGene 610L Rev 11 2 2 4 LCD LCD 6 3 P 54 FIRMWARE
7. 2 Xe 3 QuickGene 610L Rev 1 1 1 1 1 1 1 2 0 NS 3 1 3 4 1 4 L141 5 1 5 8 1 6 18 2 2 1 19 2 2 20 2 3 20 2 4 OBIO 21 2 5 EN 22 3 3 1 i 23 3 2 24 3 3 25 3 4 26 3 5 HB 27 3 6
8. QuickGene 610L Rev 1 1 53 6 6 3 LCD LCD Nw MTER HD OCR MT ER HD TB WS MT ER Ws POWER ALARM E
9. 14 QuickGene 610L Re 1 2
10. 0 5 40 QuickGene 610L Re 4 4 4 39 10 DEPC 0 1N 0 19 6EDTA DEPC
11. P9 1 N 2 CR MOVE
12. P27 i 4 la Sc 4 D EF j i a ARAL QuickGene 610L Rev 1 23 3 _ OO 1 2 3 4 5 6
13. co 9 a QuickGene 610L sei 11 1 P 9 1
14. 1 2 LCD START SW RESTART START 2
15. 3 QuickGene 610L rev 11 33 3 4 a nT 2
16. 1 3 10 000 20 000 20 000 QuickGene 610L Rev 1 41 4
17. 1 3 7 1 STOP LCD STOP START SW RESTART COVER OPEN 2 3 LCD START SW RESTART RESET LCD 4 gt P 34 38
18. 1 S P 8 1 43 P 40 4 4 P41 13 2 P24 3 N 2 4
19. 2 CR MOVE QuickGene 610L Re 17 1 1 LT LT CR MOVE _
20. 6 H 7 CR MOVE 8
21. 4 CR MOVE QuickGene 610L rev 11 35 3 5 O I 3 1 g We 3
22. 1 1 1 14ml 7ml P 25 2 4 3 4 1 14ml 7ml P 25 2
23. 3 4 5 START LCD SETUP WRITING STOP RESET SETUP COMPLETED DNA WHOLE BLOOD 48 QuickGene 610L sei 5 5 3 EXPERT
24. 1 DNA WHOLE BLOOD 19 DNA PLANT 2 ISOLATE A 20 RNA PLANT 3 ISOLATE B 21 DNA VIRUS 4 ISOLATEC 22 RNAVIRUS 5 ISOLATE D 23 SWAB 6 ISOLATEE 24 STOOL 7 ISOLATEF 25 PCR PURIFY 8 ISOLATEG 26 GEL EXTRACTION 9 ISOLATEH 27 REACTION CLEANUP 10 RNA WHOLE BLOOD 28 DYE CLEANUP 11 RNA BLOOD PLUS 29 RNA BACTERIA 12 RNA CELL 30 DNA BACTERIA 13 RNA CELL PLUS 31 BACTERIA PLUS 14 DNA TISSUE 32 DNA YEAST 15 RNATISSUE 33 DNA CULTURE CELL 16 RNA TISSUE PLUS 34 DNAHAIR NAIL 17 PLASMID 35 DNA BUFFYCOAT 18 PLASMID B 1 LCD MODE 2 4 3 LCD MODE DISCHARGE
25. 8 STOP STOP 9 RESET 10 DISCHARGE QuickGene 610L Rev 1 7 No DNA WHOLE BLOOD ISOLATE A ISOLATE B ISOLATE C ISOLATE D ISOLATE E ISOLATE F
26. ac 1 4 Rev 1 1 QuickGene 610L 1 6 7 8 9 10 11 12 3 5 6 y a 2 4 13 No 1 2 3 4
27. 1 O 24 QuickGene 610L Re 3 3 3 1 3 P8 500ml 1 5ml WTL 3 CAL2
28. lt 3 QuickGene 610L Rev 1 27 3 4 5 3 z a ST a 6 Ca 1
29. IN QuickGene 610L Re E
30. N contamination 3 1 3 P25
31. QuickGene 610L sei 1 3 2 4 oO
32. P 25 2 54 QuickGene 610L sei A A 1 1 A 2 6 P53 A 3
33. 2 QuickGene 610L Rev 1 29 3 9 e 10 1
34. oO 42 QuickGene 610L Re 4 7 WASH ELUTION WASH ELUTION
35. QuickGene 610L sei I A 2 20kg 24kg
36. 9 WASHDIE TMS 0 od WASH DIP TM 510 0 510 2 WAS2WAITT 0 10 WAS2 WAIT 255 0 255 1 WAS2 COUNT 0 11 3 i WAS2 COUNT 5 0 5 12 WAS2 PEAK 30 WAS2 PEAK 160 30 160kpa 1kpa 13 WAS2 VOL1 0 1 1 WAS2 VOL1 7500 0 lt 7500 250 14 WAS2 VOL2 0 2 2 WAS2 VOL2 7500 0
37. EXPERT 5 1 Ar E USER BEXPERT USER LCD DNA ISOLATE ISOLATE ISOLATE ISOLATE ISOLATE ISOLATE WHOLE A B C D E F BLOOD 30 1 BIND PEAK np kpa 120 120 120 120 120 120 120 160 2 WASH COUNT 0 0 3 3 3 3 3 3 3 30 3 WASH PEAK 0 kpa 90 90 90 90 90 90 90 160 4 WASH VOL1 Pe a ul 7500 7500 7500 7500 7500 7500 7500 5 WASH VOL2 bn ul 6500 6500 6500 6500 6500 6500 6500 6 WASH
38. 2 2 4 QuickGene 610L sei Rp ICG o8o9o8o9o9o9o8oSo9ooooo9ooooo8o9o9o8oSo ooo9oooooooooooooOoooooooOoooooooOoOo 68o9ooo9o9oooeo9oooooGo9oooooGo9o9oooGo oooooooooooooooooooooooooOoooooooOoOo 9o9oeo9o9oooSo9ooo9oooooooooSo9o9o9oGo ooo9oooooooooooooooooooooooooooSo9oooy eSo8o8oSoSogoooSoSogoooSo9ogoSoSo8ogoSo o5o9ooooooooooooooooooooooooooooo9oOoy 68o8o8o9o9o9o8ooSo8o8o9o8oooSo9o9o8o8o o5o9ooooo9ooooooooooooo9ooooooo9o9oOoy 9o9o8o9o9ooooo9ooooooo9oooooSo9o9oooSo oooooooooooooooooooOoooooOoooooooOoOo 5o9o8oSo9oooeooooo9oGoSo9o o9o9oSo oSo9ooooooooooooooooooooooooooooo9oooy o8o8oSoSogo8oSoo8oooSoSogoSoSo8ogogo ooo9ooooooooooooooooooooooooooo9o9oOoy o8o9o8o9o9ogoooSooooooo9oooooo9o9o8oSo o9o9oooooo
39. QuickGene 610L Re 43 4 3 4 a 5 6 7 44 QuickGene 610L sei 5
40. 3 QuickGene 610L Rev 1 15 1 16 1 2
41. 1 2 1 1
42. NM 1 Ni QuickGene 610L Rev 1 1 3 4 5 2 6 3 13 1
43. P18 QuickGene 610L Re 25 3 5 1 6 5 3 6 4 P54 7 8 9 DISCHARGE
44. DISCHARGE
45. 4 5 2 3 LCD LED START
46. 12 CRMOVE LCD 13 4A A LCD 11 MODE 1 5 P9 P9 P12 P14 500ml
47. Oo QuickGene 610L Re 1 1 1 15 30 C a RH 30 80 10 80 29 29 C Hr 12 RH day 30 m 2000
48. 1 II 2 DISCHARGE 4 DISCHARGE 3 O 4 IN 5 GY lt ee Se 1 6
49. 0 59 121 C 20 121 C 20 4 5
50. STOP 122 3 24 526 _ QuickGene 610L Rev 1 21 2 2 5 QuickGene 610L a 448mm x 332mm x 398mm 24kg AC100V 100W 0 15 30 A 30 80 RH LCD16 x 1 6 2000m 65dB A 22 QuickGene 610L Rev 1 1 3 3 lt
51. FE 3 b 2 QuickGene 610L sei
52. R 9 10 0920 6 7 8 9 6 1 5 11 12 13 QuickGene 610L Rev 1 1 1 BINDING WASHING ELUTION POWER gt gt gt CR MOVE MODE SIART READY No 1 LCD
53. 3 LCD MODE DISCHARGE EXPERT MODE Y 4 4 EXPERT EXPERT P 47 EXPERT 50 QuickGene 610L sei 5 5 4 LCD RENAME RENAME
54. 38 3 7 38 4 4 1 39 4 2 1 lk 39 4 3 40 4 4 0 41 4 5 2020 ER 41 5 5 1 0 WON 45 5 2 47 5 3 EXPERT 49 5 4 51 QuickGene 610L sei 6 6 1 96 U e KM XY 53 6 2 53 6 3 NR 54 6 4 i 54 A A 1 SS NR RE RRS SA 55 A 2 EN 55 A 3 0 55 A 4 55 QuickGene 610L Rev 11 INDEX 1 2
55. E P 45 5 VC PS 7 PROCESSING 3 6 P38
56. 5 6 START LCD PROCESSING 1 N 34 QuickGene 610L Rev 11 3 2 Oo 3
57. 2 QuickGene 610L Rei 1 1 2 2 ee ey Se WTL CAL2 QuickGene 610L sei 3 1 1 3 1 2
58. 5 2 FL QuickGene 610L Rev 1 1 1 Ng MODE 47 5 2 4 LCD SETUP START DNA WHOLE BLOOD BIND PEAK 120 3 MODE 1 DISCHARGE 4 4
59. 11 Du 6 12 30 QuickGene 610L Re 3 13
60. AC100V 15 30 C 30 80 RH 24kg OM QuickGene 610L QuickGene
61. RENAME MODE Y 4 RENAME QuickGene 610L Re 51 5 5 LCD MODE DISCHARGE 6 START LCD DNA WHOLE BLOOD RENAME Y NVY 7 LCD USER NAME WRITING RENAME STGP 32 X STOP RENAME COMPLETED
62. 2 DISCHARGE DISCHARGE P10 3 O QuickGene 610L Rev 1 1 4 N 5
63. EXPERT USER EXPERT EXPERT LCD DNA ISOLATE ISOLATE ISOLATE ISOLATE ISOLATE ISOLATE WHOLE A B C D E F BLOOD 1 BIND COUNT 1 5 1 1 1 1 1 1 1 2 BIND SPEED 1 12 12 12 12 12 12 12 12 3 BIND PEAK 30 160 kpa 120 120 120 120 120 120 120 4 BIND UP TM 6 50 sec 15 15 15 15 15 15 15 5 BIND RETRY 30 160 kpa 160 160 160 160 160 160 160 6 BIND LOWER 3 160 kpa 90 90 90 90 90 90 90 7 BIND DOWN T 2 510 sec 26 26 26 26 26 26 26 8 BINDRDNT 2 510 sec 100 100 100 100 100 100 100 9 BIND FALL 5 60 kpa 60 60 60 60 60 60 60 10 WASH COUNT 0 5 3 3 3 3 3 3 3 11 WASH SPEED 1 12 12 12 12 12 12 12 12 12 WASH PEAK 30 160 kpa 90 90 90 90 90 90 90 13 WASH UP TM 1 50 sec 15 15 15 15 15 15 15 14 WA
64. y 1 34 5_6_ 1 lt 3 4 5 6 32 17 START LCD PROCESSING LED LCD PROCESSING 3 6 P38 3 7 P38 P 33
65. 2 1 6 3 P 23 18 QuickGene 610L Re 2 2 1 1 2 SF 3 7 MNT 4 5 e IN a ig No
66. 1 LCD LED oe 2 3 4 5 QuickGene 610L Rev 1 1 19 2 2 2 1 2 3
67. 1 2 3 4 5 6 O 0O 9 x 3 2 2 O _ II O N 2 I LCD WAIT A MOMENT LCD
68. 2 4 LCD P21 6 3 P54 LED 2 POWER 3 READY 4 BINDING 5 WASHING 6 ELUTION 7 START
69. 6 28 QuickGene 610L Rev 11 3 7 2 a 1 8
70. P14 P16 P18 1 300ml 1 4 500ml 500ml BD
71. RESET 2 RENAME MODE Y RENAME USER 52 QuickGene 610L sei 6 LCD 6 1 6 2 6
72. 18 3 BINDING WASHING ELUTION LCD 0 QuickGene 610L Re 3 START 1 LCD START SW RESTARTI 8 IN 3s O 2 3
73. P16 36 QuickGene 610L Rev 1 1 3 9 3 3 P25 4 P39 1 14ml 7ml 4 1 P 39 6 4
74. 50ml 8 QuickGene 610L sei 1 N 1 2 N 1 II LCD DNA WHOLE BLOOD
75. 0 l 2 8 zan0 2 OU Sd i 0gl 3 WASH VOLS 0 ia 0 7500l 250 WASH VOL3 7500 0gl QuickGene 610L Rev 1 1 5 LCD 4 0l 5 WASH VOL5 0 RSE 0 lt 7500l 250 8 5 WASH VOLS 7500 0
76. 5 7 35 7 1 i 5 2 P47 PS1 gt S 4
77. 3 4 5 6 LCD A QuickGene 610L Rev 1 Vii Viii QuickGene 610L Re 1 1 0
78. 4 3 4 QuickGene 610L Rev 1 39 4 4 3 em lm lt PT 2 a NN 4 9 ROD r
79. 3 4 26 QuickGene 610L Re 3 3 5 1 N 2 Oo CR MOVE
80. QuickGene 610L Re 55 56 1 3 P4 QuickGene 610L Rev 1 1 Nucleic Acid Isolation System QuickGene 610L 2007 1 Printed in Japan FUJiTILM T 709101 FP 1 F UJ F LM Corporation QG610L J A016402 L8 0701 1
81. P 54 3 QuickGene 610L Rev 1 37 3 3 6 LCD COVER OPEN 1 3 7 P 38
82. Tel 03 6271 2158 Fax 03 6271 3136 Address 107 0052 9 7 3 E mail sginfo fujifilm co jp HomePage http lifescience fujifilm com A 4 I 2 20kg 24kg 1 3 P4 2 oO 1 3 P 4
83. lt 7500 250 1 WAS2 VOL3 0 3 3 WAS2 VOL3 7500 0 7500 250 16 WAS2 VOL4 0 4 4 WAS2 VOL4 7500 0 7500 250 17 WAS2 VOL5 0 5 5 WAS2 VOL5 7500 0 7500 250 ELUTVOL 50 5 1 18 1 ELUT VOL 500 1 00 50 19 ELUT PEAK 30 ELUT PEAK 160 30 160kpa 1kpa 20 ELUTDIPTM 0 AS ELUT DIP TM 510 0 510 2
84. ISOLATE ISOLATE ISOLATE ISOLATE wHoLE A B C D F BLOOD 36 WAS2 VOL2 0 7500 6500 6500 6500 6500 6500 6500 6500 37 WAS2 VOL3 0 7500 ul 5500 5500 5500 5500 5500 5500 5500 38 WAS2 VOL4 0 7500 ul 0 0 0 0 0 0 0 39 WAS2 VOL5 0 7500 ul 0 0 0 0 0 0 0 40 ELUT VOL 50 500 ul 500 500 500 500 500 500 500 41 ELUT COUNT 0 5 1 1 1 1 1 1 1 42 ELUT SPEED 1 12 12 12 12 12 12 12 12 431 ELUT PEAK 30 160 kpa 100 100 100 100 100 100 100 44 ELUT UP TM 1 255 SeC 15 15 15 15 15 15 15 45 ELUT RETRY 30 160 kpa 100 100 100 100 100 100 100 46 ELUT LOWER 3 lt 160 kpa 65 65 65 65 65 65 65 47 ELUT DOWNT 2 lt 510 SeC 20 20 20 20 20 20 20 48 ELUTRDNT 2 lt 510 SeC 30 30 30 30 30 30 30 491ELUT FALL 5 60 kpa 40 40 40 40 40 40 40 501ELUT DIP TM 0 lt 510 SeC 0 0 0 0 0 0 0 51 W DSVOL S 5000 15000 gl 5000 5000 5000 5000 5000 5000 5000 52 E DSVOL S 2200 6000 ul 2200 2200 2200 2200 2200 2200 2200 53 BUZZER ON or OFF ON ON ON ON ON ON ON 54 DETECT PRES 1 50 kpa 15 15 15 15 15 15 15 55 DOWN PRES 10 50 kpa 50 50 50 50 50 50 50 EXPERT EXPERT 1 MODE 2 4
85. VOL3 A A ul 5500 5500 5500 5500 5500 5500 5500 7 WASH VOL4 AR ul 0 0 0 0 0 0 0 8 WASH VOL5 ul 0 0 0 0 0 0 0 9 WASH DIP TM sec 0 0 0 0 0 0 0 10 WAS2 WAITT min 0 0 0 0 0 0 0 QuickGene 610L Rev 1 45 46 5 DNA ISOLATE ISOLATE ISOLATE ISOLATE ISOLATE ISOLATE LCD a WHOLE A B C D BLOOD 11 WAS2 COUNT 0 0 0 0 0 0 0 0 5 5 30 12 WAS2 PEAK kpa 90 90 90 90 90 90 90 0 13 WAS2VOL1 Al 7500 7500 7500 7500 7500 7500 7500 0 14 WAS2 VoL2 il 6500 6500 6500 6500 6500 6500 6500 0 15 WAS2 VoL3 gl 5500 5500 5500 5500 5500 5500 5500 0 16 WAs2 VoL4 7500 0 0 0 0 0 0 0 0 17 wAszvoL5 900 0 0 0 0 0 0 0 50 18 ELUTVOL 500 500 500 500 500 500 500 500
86. VxxLxx WAIT A MOMENT DNA WHOLE BLOOD DNA WHOLE BLOOD ISOLATE A ISOLATE A ISOLATE B ISOLATE B ISOLATE C ISOLATE C ISOLATE D ISOLATE D ISOLATE E ISOLATE ISOLATE F ISOLATE F PROCESSING lt 3 COMPLETED 1 RL RE DT
87. 3 1 2 CR MOVE 15 16 MODE QuickGene 610L Re 31 3
88. 30 19 ELUT PEAK kpa 100 100 100 100 100 100 100 1 0 20 ELUT DIP TM si sec 0 0 0 0 0 0 0 LCD 4 BIND PEAK 30 BIND PEAK 160 30 160kpa 1kpa WASH COUNT 0 2 WASH COUNT 5 0 5 3 WASHPEAK 30 WASH PEAK 160 30 160kpa 1kpa 1 TI 1 7500 250
89. EPROM ER EEPROM HISTORY DATA ERR CARRIER POS ERR NOZZLE POS ERR TABLE POS ERR LIFT POS ERR WD PUMP ERR CD PUMP ERR PRESSURE ERR COVER OPEN STOP SW PUSHED STOP RESET 6 4 o 1 4
90. Life Science FUJIFILM Nucleic Acid Isolation System QuickGene G10L Rev 1 1 Nucleic Acid Isolation System QuickGene 610L DNA RNA
91. QuickGene 610L Rev 11 4 4 6 4 1 AN 4 3 P40 1 4 2 1
92. SH RETRY 30 160 kpa 90 90 90 90 90 90 90 15 WASH LOWER 3 160 kpa 80 80 80 80 80 80 80 16 WASH DOWN T 2 510 sec 20 20 20 20 20 20 20 17 WASHR DN T 2 510 sec 100 100 100 100 100 100 100 18 WASH FALL 5 60 kpa 40 40 40 40 40 40 40 19 WASH VOL1 0 7500 ul 7500 7500 7500 7500 7500 7500 7500 20 WASH VOL2 0 7500 ul 6500 6500 6500 6500 6500 6500 6500 21 WASH VOL3 0 7500 ul 5500 5500 5500 5500 5500 5500 5500 22 WASH VOL4 0 7500 ul 0 0 0 0 0 0 0 23 WASH VOL5 0 7500 ul 0 0 0 0 0 0 0 24 WASH DIP TM 0 510 sec 0 0 0 0 0 0 0 25 WAS2 WAIT T 0 255 min 0 0 0 0 0 0 0 26 WAS2 COUNT 0 5 0 0 0 0 0 0 0 27 WAS2 SPEED 1 12 12 12 12 12 12 12 12 28 WAS2 PEAK 30 160 kpa 90 90 90 90 90 90 90 29 WAS2 UP TM 1 50 sec 15 15 15 15 15 15 15 30 WAS2 RETRY 30 160 kpa 90 90 90 90 90 90 90 31 WAS2 LOWER 3 160 kpa 80 80 80 80 80 80 80 32 WAS2 DOWN T 2 510 sec 20 20 20 20 20 20 20 33 WAS2R DN T 2 510 sec 100 100 100 100 100 100 100 34 WAS2 FALL 5 60 kpa 40 40 40 40 40 40 40 35 WAS2 VOL1 0 7500 ul 7500 7500 7500 7500 7500 7500 7500 QuickGene 610L Re 49 5 LCD DNA ISOLATE ISOLATE
93. a 10 QuickGene 610L Rev 11 1 b WASH H ELUTION WASH ELUTION 8 bh
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