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Kudoa septempunctata (18S rRNA) qPCR

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1. http wwwr takara bio coJp 5 RR260A http wwwr takara bio coJp 6 RR260A NOTICE TO PURCHASER LIMITED LICENSE A6 PCR Notice Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the us 5 804 375 5 538 848 5 723 591 5 876 930 6 030 787 and 6 258 569 The purchase of this product includes a limited non transferable immunity from suit under the foregoing patent claims for using only this amount of product solely in Environmental Testing Food Testing Industrial Microbiology including reporting results of purchaser s activities for a fee or other commercial consideration and also for the purchaser s own internal research No right under any other patent claim is conveyed expressly by implication or by estoppel Further information on purchasing licenses may be obtained from the Director of Licensing Applied Biosystems 850 Lincoln Centre Drive Foster City California 94404 USA L15 Hot Start PCR Lic
2. 4 w 3 1 2 3 5 wm PCR PCR Auto PCR Manual
3. 4 6 20 C http wwwr takara bio coJp 2 RR260A PCR Thermal Cycler Dice Real Time System TP900 TP960 Thermal Cycler Dice Real Time System Lite TP700 TP760 Applied Biosystems 7500 Fast Real Time PCR System Life Technologies 200 uk 20ulk 10ul IV 1 PCR 2 3 Ww
4. http wwwr takara bio coJp 3 RR260A V X DNA Kudoa septempunctata 0711 1 1 3 Kudoa Positive Control EASY Dilution for Real Time PCR 2 5 X 107 2 5 X 105 2 5 X 103 2 5 x 10 copies ul 1 4 1 x 108 1x 106 1 x 104 1 x 102 2 1 o 16 ul 1 4 Thermal Cycler Dice Real Time System 1 Premix Ex Taq Tli RNaseH Plus 10 ul Kudoa Primer Probe Mix 2 ul dH20 4 ul or
5. Tel 077 543 6116 Fax 077 543 1977 http www takara bio co jp DIINTA RAS At v201302Da
6. 95 30 2 Step PCR 45 95 5 60 C 30 FAM SYBR 5 X Kudoa septempunctata 0711 1 VI Thermal Cycler Dice Real Time System TP900 TP960 Thermal Cycler Dice Real Time System Lite TP700 TP760 Thermal Cycler Dice Real Time System Single TP850 TP870 Premix Ex Tag Probe qPCR RR390A B EASY Dilution for Real Time PCR 9160 NucleoSpin Tissue 740952 10 50 250 Vil
7. or 4 ul 20 ul Life Technologise PCR 1 Premix Ex Taq Tli RNaseH Plus 10 ul Kudoa Primer Probe Mix 2 ul ROX Reference Dye or Dye Il 2 04 ul dH20 3 6 ul or or REK 4 ul 20 ul 1 2 StepOnePlus ROX Reference Dye 7500 Fast Real Time PCR System ROX Reference Dye ll 3 DNA 3 2 DNA 4 ul 0 2 ml PCR http wwwr takara bio coJp 4 RR260A 4 PCR PCR PCR PCR
8. ensed under U S Patent No 5 338 671 and 5 587 287 and corresponding patents in other countries L53a Rox Reference Dye Application Field Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US 5 928 907 The purchase of this product includes a limited non transferable immunity from suit under the foregoing patent claims for using only this amount of product solely in Environmental Testing Industrial Microbiology including reporting results of purchaser s activities for a fee or other commercial consideration and also for the purchaser s own internal research No right under any other patent claim is conveyed expressly by implication or by estoppel Further information on purchasing licenses may be obtained by contacting the Director of Licensing Applied Biosystems 850 Lincoln Centre Drive Foster City California 94404 USA M40 Thermostable RNaseH This product is covered by the claims of U S Patent No 7 422 888 and its foreign counterpart patent claims M57 LA Technology This product is covered by the claims 6 16 of U S Patent No 5 436 149 and its foreign counterpart patent claims M82 Tli RNaseH Plus This product is the subject of the pending JP patent application http wwwr takara bio coJp 7 RR260A Takaka
9. wm RR2Z60A IdfaR4 Kudoa septempunctata 18S rRNA qPCR Detection Kit v201302Da Kudoa septempunctata 0711 1 PCR Kudoa septempunctata PCR L 20 ul 50 1 Premix Ex Taq Tli RNaseH Plus 2 X conc 500 ul 2 Kudoa Primer Probe Mix 10 X conc 1 100 ul 3 dH20 1 ml 4 ROX Reference Dye 50 X conc 20 ul 5 ROX Reference Dye II 50 X conc 20 ul 6 Kudoa Positive Control 2 5 X 107 copies ul 2 50 ul 7 EASY Dilution for Real Time PCR 1mlx 2 1 FAM TAMRA x2 PCR 1 5 ll 20 C Premix Ex Taq Tli RNaseH Plus 4

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