ミニプロティアンゲル クイックガイド - Bio-Rad
Contents
1. 2 TGX IPG Well Prep 10mm 5mm TGX IPG 1 0 5 1 0 BPB 10mm 5mm TGX 7cm IPG IPG2. 2 1 9 a Se LL Tetra 3 2 2 140ml 2 3 Tetra 2Gels 4Gels 3 Tetra 1 gt 3
3. 4 100 200V 10 100mA 5 6 7 456 0000 3 1 3 2 gt TGX 200V 30 40 Peptide 100V 100 TBE 100V
4. 1 3 Tetra 2 TGX Peptide TBE 20 4 1 1 1 1 2 1 3 NSR III ll ll ll ll 3 4 Pd a m 1 4
5. TGX TGX IPG IPG 2 3 4 5 6 Visit us at http www bio rad com 2 Peptide 3 TBE 5x DNA 30ml 200 mM Iris HOCL pH 6 8 24 SDS 40 glycerol 50mM Tris HCI pH 8 0 5mM EDTA 25 glycerol 0 0 0 6 0 2 bromophenol blue 0 2 xylene cyanole FF 26 2 ME 350mM DTT 161 0767 5x DNA 30ml 161 0739 30ml 2 a 4 1
6. 400V TGX 1 3 1 2 Turbo TGX SD TGX 15V 30 15V 15 20 TGX 100V 60 100V 30 40 2 gt 1 TGX Laemmli like SDS
7. 4 A Wn Ne 6 4 a 3 3 i Wm 4 3 2 3 2 2 8 85 x 100cm Hx W 1 12 12 0 02 1 TGX SDS PAGE 2x Laemmli 62 5mM Tris HCIl pH 6 8 2 SDS 25 glycerol 0 01 BPB 5 2 ME 350mM DTT
8. 1 lt 5 UV 1 UV UV CCD UV rr ye PVDF Ordering Information TGX meee oe ee Ou Ou 20 su 7emmpGsStip 450ul 10 456 1023 456 1033 456 1043
9. SDS PAGE Native PAGE 2 Peptide 3 TBE DNA 2000bp TBE Native PAGE 62 5mM Tris HCI pH 6 8 40 glycerol 0 01 BPB 161 0738 Native 30ml 1 1 25mM Tris 192mM glycine pH 8 3 161 0734 10 1L 161 0771 10 5L 10 200V 30 40 CBB R 250 CBB Bio Safe CBB G 250 Dodeca Zinc Negative 2 RUBY Flamingo Oriole 1
10. 4 1 1 1 89mM Tris pH8 3 89mM boric acid 2mM EDTA 161 0733 10xTBE 1L O Oo 2 PF lz RN AA NG 2 10 100mM Tris 100mM Tricine 0 1 SDS pH8 3 100V 45 100 8 15mA gel 161 0744 1 tp 10 x Tris Tricine SDS 1L 2Gels 10 i ey 3 SYBR 100V 100 NN SYBR Green 65mA gel 2Gels 4Gels CBB 40 10 30 Bio Safe CBB 1 2 ee 2 3 1
11. TGX 5 Peptide TBE 1h 18 ny kH 155 2 1 16 5 10 20 5 10 15 4 20 2 000 m 500 250 2 000 1500 1 000 250 250 250 150 250 150 1 500 1 000 700 mm 1200 150 500 m 150 700 400 1 500 m 150 100 150 100 1 000 m Th 15 700 m 400 a 100 106 6 20 700 100 75 100 a a AR 500 75 2 15 wm 20 400 rb 400 200 50 7 3r Es i100 300 mm ci 300 aa 87 9 5 10 0 7 100 mw 50 ri Se 2 37 pa 200 100 sa an 20 37 ss 0 2 50 50 15 8 15 25 a 15 100 FT 1 1 1 1 0 1 50 kDa kDa bp 456 0000 165 3320 3 gt Visit us at http www bio rad com TGX Stain Free 1 Stain Free TGX Stain Free
12. 161 0737 2x Laemmlii 30ml 161 0747 4x Laemmlii 10ml 95C 5 2x Laemmli 1 1 4x Laemmli 3 1 25mM Tris 192mM glycine 0 1 SDS pH 8 3 161 0732 10x SDS 1L 161 0772 10 x SDS 5L 10 200V 30 40 40 50mA gel 2Gels 4Gels Rapid 300V 15 20 50 80mA gel 4Gels
13. 45 100 3 3 TGX Peptide TBE 4 1 4 2 456 0000
14. 456 1083 456 1093 456 1103 456 9033 456 1024 456 1034 456 1044 456 1084 456 1094 456 1104 456 9034 456 1025 456 1035 456 1045 456 1085 456 1095 456 1105 456 9035 456 1026 456 1036 456 1046 456 1086 456 1096 456 1106 456 9036 456 102 1 456 1031 456 1041 456 1081 456 1091 456 1101 456 9031 30 456 XXXXB03 B03 50 456 XXXXB05 B05 TGX StainFree te a Ou Ou 20 su 7emmpGsStip 450ul 10 456 8023 456 8033 456 8043 456 8083 456 8093 456 8103 456 8123 10 456 8024 456 8034 456 8044 456 8084 456 8094 456 8104 456 8124 10 456 8025 456 8035 456 8045 456 8085 456 8095 456 8105 456 8125 456 8036 456 8046 456 8086 456 8096 456 8106 456 8126 10 456 8021 456 8031 456 8041 456 8081 456 8091 456 8101 456 8121 Peptide TBE 30 50 456 3063B02 456 3065B02 456 3113B02 456 3114B02 456 3115B02 456 3116B02 456 5013B02 456 5014B02 456 5015BO2 456 5016BO02 456 5033B02 456 5034B02 456 5035B02 456 5036B02 456 5053B02 456 5054B02 456 5055B02 456 5056B02 Tel 03 6361 7000 Fax 03 5463 8480 456 5093B02 456 5094B02 456 5095B02 456 5096B02 Tel 03 6404
15. 0331 Fax 03 6404 0334 lt 4 gt M10617 1405A Visit us at http www bio rad com 140 0002 2 2 24 20 Peptide 16 5 10 20
16. UV Stain Free CBB 2 Stain Free Plus 161 0363 Plus 161 0373 1 1 3
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