DNAメチル化 - コスモ・バイオ
Contents
1.2. 7
3. RCAS Vo HCAS 86
4. Cosrmo Bio News No 76 27 AARSD1 AASS ABC 50 ABCC8 ABL ACC1 ACIN1ACIN1 ACLY ACO 1 ACP6 ACTA2 ACTN2 ACTR 3 ACTR IB ADD2 ADIPOR 2 AMY1A ANKRD15 ANP 32A ANPEP AP1S2 AP2S1 APAF 1 ALT APBA1 ARHGAP25 ARID 5B ARMC1 Arrestin 1 ARSG ASPH AurA AurB AurB C AZGP 1 BAL BBS10 BCAP 29 BCAS 1 BCDO2 Bcl 2 BEGAIN BERP RNF22 BIM c B BIm 10 BMP 8A BPIL1 BRAG BSA BSCL2 BTK BZW1 CACNB4 CACNGS3 CALB1 CaMKIIB y CAMKK1 CARS CARS2 CASP10 Catenin a 1 2 Catenin y PLAK CAV1 CHAD ChAT CHCHD1 ch TOG Circumsporozoite CLDN5 Cleaved Aggrecan CNN1 CNTF COG8 COL23A1 COL25A1 COLS3A1 COMMD7 COPI CORO1C CPA6 CPN cat CPN1 28 Cosrmo Bio News No 76 A PG ABG BET ABG BWT BWT APB PGI ABG PGI ABG PGI PGI BWT PGI PGI ABG ABG ABG PGI ABG A
5. RCAS RCAS 25 2 0 2 1 6 1 5 LO 12 1 05 0 8 0 0 4 0 01 100 10000 1E 06 0 001 0 1 10 1000 agonist nM antagonist uM 3 RCAS PPAR RCAS LXR 8 TO0070907 IC50 TO901317 EC50 Troglitazon EC50 1 nM 600nM
6. DCB DPE 1020 2 10x10 cm 303111 1 set 45 000 22 Cosrmo Bio News No 76 4 70C 196 5 N 9 3 NM CFTM Dyes A Biotium 7 3 1 2
7. ALADO PCR ALADO 1 2 21 1 ALAH ALADO ALADO Ss 2 3 ALADO
8. 48 ye 9 23 1 24 1 y NEW FL EEE RCAS y awa A 7 a 2 TMB 3 ELISA 96 50my 1 RCA5
9. DNA DNMT DNA DNA DNMT 1 0 DNMT Hypomethylating drug 5 aza 2 deoxycytidine ICM Kit for DNMT1 ICM Kit for DNMT1b ICM Kit for DNMT2 ICM Kit for DNMT3a ICM Kit for DNMT3b DNA RNA DNA 182 172 15 13 Vivo DNA DMT DNA DMT a DNA DMT lt Weak complex Covalent complex 1 i Adducts npound DNMT On Free DNA DNA DMT Covalent complex DNA DNA AzaCdR DNA DNMT comp
10. 4 7 8 18 19 80 DNA 7 G48 TSGs CpGls 1 TSG CDGI 78 7 2 224 8 9 TSGs 5
11. 4 1 G 5 HRP Antibody HRP 1 SeltikinK mp4FB modified HRP x50 2 PBS pH 6 5 12 1 MDMF anhydrous 1X100A Zeba Spin Column 4 Conjugate Spin Column Collection tubes 1 NFlash Drive 1 Diafiltration spin filters PET we we Ee 2224 HydraLinkrM HyNic 4FB HRP IgG HRP Solulink Biosciences Inc SLK A 9002 001 1 kit 135 000 2 WConjugation Additive 1 x20 25
12. 0kD dsRHNA Phusion High Fidelity DNA Polymerase 10mM dNTP mix 20u8 05xPhusion HF buffer 1 5mg mLambda control DNA 0 5ng u8 Control Primer mix 25uM each 2048 Phi6 RNA Heplicase 60A 17 RNA Polymerase New England Biolabs 60 Replicator M RNAi Kit 18 Cosrmo Bio News No 76 MPyrophosphatase 40 10xdsRNA Synthesis Buffer 250 5xNTP mix 400 MM50mM 5 17 promalter wpw C Tramscriplinn promaolter 3 FeplicatorTV RNAi dsRHNA Transcription T7 RNA DNA ssRHNA Replication ssRNA Phi6 RNA dsRNA Finnzymes Oy FZM F 0 40 rxn 55 000 lt 3 4C 20CC
13. CAC Anti bFGF MKM 100 Lug 58 000 Anti fibrinogen MKM M02 100 Lg 60 000 Anti c myb 1 1 1 MKM M03 200 50 000 5 1 MKM M04 200 Lg 50 000 Anti B 1 receptor 2B9 MKM M05 100 Lug 80 000 2B12 MKM M06 100 Lg 80 000 Anti M2 receptor 1C10 MKM M07 100 Lg 80 000 Sno 1 1 MKM M08 100 Lug 80 000 3 1 MKM M09 100 Lg 80 000 1 4 MKM M10 100 Lg 80 000 Anti Ski 9 1 MKM M11 100 Lug 80 000 11 1 MKM M12 100 Lg 80 000 16 1 MKM M13 100 Lg 80 000 Anti PF 4 2D6B MKM M14 100 Lg 60 000 10E6 MKM M15 100 Lg 60 000 Elastase XDP 123 MKM M16 100 Lg 80 000 Anti Hyaluronidase MKM P01 50 80 000 CAC CAC CosmoBio Antibody Collection CAC
14. mlHFP shRNA GFP 0HF shRNA http www origene com abca1 Human RNAi Mouse RNAiI hr Cnt rm sm AFPrsuch Fr Dt TTF LB U Iimmarker validation via TS 5 IP thousands of ee i DF Foes i si Li Ba Pe ry a Fd EF Fr re Fn ir ety posed Rm rr he pr et IE mm ETT TTT E Go 164 000 1kit 0 dd
15. httD wWww cosmobio co jp company tools cbtools top asp AAAS Science Signaling sr es http www cosmobio co jp aaas_signal index asp Science Signaling Science Signaling 3 8 2009 5 7 1 J use 6
16. 0 Cosrmo Bio News No 76 dd 4C 50C 5 196C NEW ApoE ApoE 19 299 apolipoprotein E 4 ApoE 3 E E3 E4 3 3 Apo E 3 4 4 3 gpo 3 E4 E4 3 1182 158 apo Ed3 E4 apo E3 3
17. RCAS RCAS 1 RCAS RCAGS RCAS 4 C RCAS RCAS EC50 IC50 12 8 EC50 IC50 EC50 IC50 2 GLP
18. NEW EAE CIA EAE Hooke Laboratories Inc PTX dose SE MOGss ss CFA Emulsion PTX 10 81 000 PTX MOGss ss CFA Emulsion PTX 1 5x C57BL 6 EK 0110 10 test 81 000 PTX MOGss ss CFA Emulsion PTX 2 5x C57 BL 6 EK 0112 10 test 88 000 PTX MOGss ss CFA Emulsion PTX 3 75x C57BL 6 EK 0114 10 test 95 000 PTX MOGss ss CFA Emulsion PTX 5x C57BL 6 EK 0115 10 test 98 000 PTX PTX pertussis tOxin
19. EAE CIA hN2 hESC WA09 PROTEON EXPRESS 10 PhireT PCR PCR Harris Uni Core M
20. 2 1 mM 0 mW Axygen Scientific Inc AXY 5 82 AxyVac Vacuum Manifold AP VM Ye2 500 Cosrmo Bio News No 76 NEW HuSH 29 shRNA pGFP V RS pRFP C RHS 4 ShRNA 5 g ready to use HuSH 29 DRS shRNA GFP
21. ALADO ALADO P13 15 P15 24 P25 2 P26 Cosrmo Bio News No 76 2 58 X x NEW 16 1 ORIGENE CellPainterrM
22. DNA WSDS Native PAGE DNA 9 Laemmli 117 1127 55 pt 1 7 8 3 2 a 85 W x90 L x0 9 1 100 W x100 L x3 1 t 1 DCB 5 2 15 13W 2 15 30 500K 200 2 000 414855 9 800 2 2 15 17W 414862 9 800 2 4 20 13W 13 4 20 15 250K 40 1 800 414879 9 800 2 4 20 17W 17 414886 5 9 800 5 10 13W 13 5 10 35 450K 441776 5 9 800 2
23. http Www finnzymes Com directpcr index html 2 7 Direct protocal 2 re 1 1 Hilulinn protocol ilutirsm 1 6 PCR PCR POR gt lt 9 PCH PCR a a a PC PCR 5 9 1 5 PCR PCR DNA DNA Finnzymes Oy FZM es Phire Plant Direct PCR Kit F 35 000 Harris
24. Y050132 67 000 RabMAb 51 8 o R g gt PNBM gt J Allen et al Nature Methods 007 amp NO2 EPITOMICS INC EPT Anti Thiophosphate Ester Specific Mono Human Mouse Rat 60 000 6 1 CAC bFGF FM 1 SnO N C SKI XDP HYAL
25. AGEs AGEs AGEs ELISA AGEs AGEs AGEs HRP AGEs HRP 4815 0 AGEs ELISA 1 108 000
26. MethyIMagent MV mCpG DNA MM101 K 54 MethyIMagnet V mCpG Island Primer Kit MethyIMagnet V mCpG Island Primer Kit BRCA1 MethyIMagnet V mCpG Island Primer Kit CCNA1 MethyIMagnet M mCpG Island Primer Kit CDKN2A P16 MethyIMagnet V mCpG Island Primer Kit DAPK1 MethyIMagnet V mCpG Island Primer Kit DKK3 MethyIMagnet V mCpG Island Primer Kit ESR1 MethyIMagnet V mCpG Island Primer Kit FAM127A MethyIMagnet V mCpG Island Primer Kit FHIT MethyIMagnet M mCpG Island Primer Kit GAPDH Negative control MethyIMagnet V mCpG Island Primer Kit GSTM1 MethyIMagnet V mCpG Island Primer Kit GSTP1 MethyIMagnet V mCpG Island Primer Kit hMLH1 MethyIMagnet V mCpG Island Primer Kit HOXA2 MethyIMagnet V mCpG Island Primer Kit MCAM MethyIMagnet mCpG Island Primer Kit MGMT MethyIMagnet V mCpG Island Primer Kit MLH1 MethyIMagnet V mCpG Island Primer Kit PAK3 MethyIMagnet V mCpG Island Primer Kit PR MethyIMagnetTV mCpG Island Primer Kit PTGS2 COX2 MethyIMagnet V mCpG Island Primer Kit RASSF1 MethyIMagnetTV mCpG Island Primer Kit SNRPN Positive control MethyIMagnet V
27. ar DNA CO WMDNase 1 HHNase DNA Proteinase K DNA 250 mg 50 Lg 600 30 HNA DNA 6G5400 6450 5 RNA DNA
28. 4 G0O 198 N igeSt 3 FastDigest A 5 Fermenfas 7 1 5 _ 1 DNA 7 M GeneRulerTVExpress DNA Ladder SM1551 WM 5 pJET pJET Fast Digest Not 3 pJET Fast Digest Bam 4 pJET Fast Digest Not Bam Double Digestion 5 pJET 7 Fast Digest Not Bam 2 1 5 GeneJETrV DNA 0 2b Fast Digest
29. 1 amp 2 DNA RHNA DNA RNA 52 Norgen Biotek Corp Animal Tissue Total RNA Purification Kit 2 50 prep 44 000 Water RNA DNA Purification Kit 0 22 um 26400 25 prep 64 000 Water RNA DNA Purification Kit 0 45 26450 25 prep 661 000 Soil DNA Isolation Kit 26500 50 prep 32 000 Norgen Biotek Corp Total RNA Purification Kit 50 prep 4 45 000 RNA Protein Purification Kit 23000 20 prep 39 000 RNA DNA Protein Purification Kit 23500 20 prep 48 000 All in One Purification Kit 24200 25 prep 52 000 FFPE RNA Purification Kit 25300 50 test 64 000 microRNA totalIRNA genomicDNA 1 14 Cosrmo Bio News No 76 9
30. 1 LiPA probe assay DNA 5 8 45 3 DNA 20 4 1 20 INNO LiPA Apo E 8 ApoE gz 4 INNOGENETICS 1 apoE DNA ApoE 4 P
31. 4C G0C 70C 196 5 N miRNASelectrV miRNA A Pre miRNA J enous ne 7 microRNA miRNA mRNA 18 24 RNA RNA RNA induced silencing Complex RISC mRNA miRNA mRNA miRNA 35 miRNA miRNA miRNA
32. 3 PCR ChIP IgG TRANSCRIPTION FACTOR BINDING amp MRNA EXPRESSION ANALYSIS 8 7 A549 HepG2 5 5 9 amp ChIP PCR 0 0 8 p53 Binding mRNA Expression CDKN1A ChIP p53 5 FIuorouracil 5 FU CDKN1A
33. miRNA miRNA 1 miRNA me miRNA Sanger s miRNA pUCori 7 1 miRNA Blast PCR amp Human a rglobin 100base miRNA NE Intron Ampr pa BamH miRNA 6 gi 5 Precursor Nhe PCR miRNASelectm 40 PolyA 2 CFP Puro miRNA pEP miR miRNA pUC ori J pEGP miR let7 MIR mir 999 Intron let7 Amp Er mi MMU MI
34. 4C 60C 70OC 196C N uu PHOTEON EXPRESS 5 A 18 896 29 6 5 ZE PR L25 ZE PR 065 PROTEON LactogloDulin EXPRESS PROTEON OVO EXPRESS PROTEON Gluten EXPRESS Ko Sample Extraction Buffer 7 Extract Results 35 102 Am ilution buffer nutes
35. True0RHF cDNA GFP RRFP cDNA LCK Origene Technologies Inc ORG Memmibrane Proatei Autophagosome 656 LMrnFi 6
36. 0 cfu m2g gt NH 0157 5002 25 0 157 NH 026 500 2 25 026 M NH 01 1 1 500 2 25 01 1 1 1 NH Step 1 Step Step 3 1 m2 NH 0 NH Step Step4 0 1 2 1 m6 3 5 2 Step 5 Step 6 Step 7 NH NH
37. PCH microRNA DNA Et ee ti SABiosciences Corporation EE 82 GFP Lipid kinase assays PI3 Kinase and Sphingosine Kinase 1 James Thompson Director of Business Development Echelon Biosciences Inc
38. 1 109 10 3 8 10 21 10 24 Cosmo Bio News No 76 33 1 7 5 72 4 2009 9 1 6 8 8 5 10 7 2 5 135 0016 2 2 20 URL
39. ALADO EmStat EmStat USB PS Lite Windows 2000 XP Vista 0 USB MEmStat 6 1nA 100uA 1pA 0 DNA DNA DNA
40. CpG DNA Input 2 22 PCR DNA input DNA 1 10 Mock 37C 8 hr ovemight RT SYBR Green qPCR Master Mix 2 PCR PCR 6 ae PCR Double HE Methy Profilerrw PCH 4 96 DNA
41. 3 VeriKine HS IFN B Serum ELISA Kit a 30 Cosrmo Bio News No 76 IFN g 100 000 pg mg HRP TMB PBL vs CompetitorT 1 Blue PBL s New Human IFN 8 ELISA kit 0 1 1 10 1000 1 VeriKine HSTV Human Interferon Beta Serum ELISA Kit VeriKine HSTM Human Interferon Beta Serum ELISA Kit PBL InterferonSource 3 1 1x96 176 000 C PI3 Kinase ELISA Pico Assay PI3 K PIG PI 4 5 P 6 PI 3 4 5 P3 PtdIns 3 4 5 Pd PIG 4 5 Pd
42. MethyIMagnet M GST MBD DNA CpG CpG mCpG MBD gt Glutathione beads 4 DNA DNA DNA DNA 5 DNA A GST MBD mCpG DNA B DNA GST MBD K DNA GST MBD A B D E B Glutathione beads MBD mCpG DNA E
43. PI3 K P 4 5 P dICB PI 3 4 5 PS diC8 PI3 PI 3 4 5 PS 0 10XTBS T ELISA Pico MBB 1 N HsS04 100mM EDTA 5 lt KBZ 3 4 5 P3 Standard Curve PI 0 PIG45 P9 Pml 1 PI 3 4 5 P3 Echelon Biosciences Inc ECL PI3 K ELISA Pico
44. DNA GST GST GST GST GST DNA GST 1 MethyIMagnet V mCpG DNA Isolation Kit Thrombin Cleavage Site A GST Domain LVPRUGSPGISGGGGGIR MBD B DNA Binding Region on Surface MBD Cysteineson GST gt Linker 2 MethyIMagnetrM 1 DNA DNA DNA DNA slTictian 3 DNA DNA DNA
45. VvoIl 9 2 7 2009 2010 1727 717 2008 2010 FAQ Pp 6 Tool for school 58 DNA Methylation Analysis and Expression Profiles of Human MicroRNA in Cancer
46. siHRNA siRNA PowerCut M Dicer ReplicatorrW RNAi 7o dsRHNA siRNA siRNA 10096 MSiRNA overdigestion 5 7 SIRNA Dicer dicer 1 overdigestion 10096 siRNA PowerCut Dicer 2 3 dsRNA Furin eGFP phi6 dsRNA siRNA PowerCutrM Dicer 10096 3 RNase siRNA SIRNA 10nM
47. 8 Nested PCR 9 alt i a HBV DNA 02808853 080838 258 bh HBV DNA 6 HBV DNA 281 1 ALADO APE 02 100 pc 25 000 ALADO AOC 02 15 pc 7 500 ALADO ACS 02 1 pc 1 000 ALADO AH 02 1 pc 400 222 HA C02 1 pc 2 000 HAP 02 1 pc 17 000 EmStat instrument USB version EMSTAT 1 set 350 000 26 Cosrmo Bio News No 76 vol 33 3 7 PD
48. ali Corporation SPA A Methyl ProfilerrV PCR MePH A 2 42 000 MM SABioscience http Wwww sabiosciences Com dna_methylation_qPCRprimer php Methly ProfilerTM 2 MENS SABioscienco Corporation Methyl Profiler M DNA Methylation Enzyme Kit 1 kit 12 5 42 000 PCR http Www cosmobio CO jp product raku 00880004 asp 2 Cosrmo Bio News No 76 4C 70C 19 02 ChampionChIP M 3 N A ChIP ChIP PCR SABiosciences ChIP amp
49. rr rs tr His Myc HA GFP HFP YFP Technologies Inc ORG PrecisionShuttle V pCMV6 AC Destination Vector PS100010 10 Lg 88 000 PrecisionShuttle V pCMV6 AN GFP Destination Vector nm PS100019 10 Lg 88 000 PrecisionShuttle TV pCMV6 AC RFP Destination Vector C PS100034 10 g 88 000 PrecisionShuttle V pCMV6 AN RFP Destination Vector N PS100033 10 Lug 88 000 8 Cosrmo Bio News No 76 1 DiRas3 N GFP DiRas3 N RFP MAP LCS GFP Golgl Apparatus LaIHIE1 Ri Endosome fl AL RR 31 mm Endoplasmic Reticulum GA Focal Adhesion Fiber FAR WEL WW Etim Fibers 122 ACTB Focal adherin fiber Golgi apparatus Lysosome Mitochondria Neural axiS Nucleus Peroxisome Plasma memberane Synaptic vescles PCMV6 AC GFP Neuronal Markers GAP43 Autophagosome CO IOocalized with autophagy Inducer DiRas3 HRC10001 1 RC100043 TECHNOLOGIES IN
50. 1 WB Columbia Bio LLC COM Universal One step Western Blot Kit Mouse With DAB Substrate DAB WB M50 1 kit 27 000 Universal One step Western Blot Kit Rabbit With DAB Substrate DAB WB R50 1 kit 27 000 Universal MV One step Western Blot Kit Goat With DAB Substrate DAB WB G50 1 kit 27 000 Universal One step Western Blot Kit Mouse With ECL Substrate ECL WB M51 1 kit 32 000 5 UniversalTM One step Western Blot Kit Rabbit With ECL Substrate ECL WB R51 1 kit 32 000 Universal MV One step Western Blot Kit Goat With ECL Substrate WB G51 1 kit 32 000 2 24 Cosrmo Bio News No 76 0 dd 4C 50C 70C 196 NEW
51. Mitochorndria EOHA LS some LAMP1 Coated Pit Autophagosome ATG12 ns RC100004 Di Ras3 RC100011 MAPILC3A 2 RC100020 MAPILC3B N RC100021 Centrosome PLK1 N RC100023 Coated pit CLTB N RC100010 Cytoskeleton ACTB N RC100002 MAP EB3 C RC100019 PFN1 N RC100022 TUBA1B N RC100030 Endoplasmic reticulum CALR C RC100008 Endosome Rab4 N RC100025 Rab5 N RC100026 RhoB N RC100027 1325 000 10 g MIlul CellPainter Vector 4 FMFS RC100036 RC100043 RC100052 RC100053 RC100055 RC100042 RC100034 RC100051 RC100054 RC100062 RC100040 RC100057 RC100058 RC100059 Other Cytoskeleton Proteins PEM1 CellPainter Vector PrecisionShuttle Vector TrueURHF PrecisionShuttlerM Fe 8 PrescisionShuttlerw
52. WMethyLProfilerrw PCH DNA PCR Methly ProfilerrM PCR K 1 96 A C D F 2 PCR ei Human Breast Cancer ee 1X Y MeAH 8010X Y MeAH 3010X Y A D 2 105 000 Human Gastric Cancer MeAH 021X Y MeAH 8020X Y MeAH 3020X Y 12 1 336 000 Human Liver Cancer MeAH 031X Y _ MeAH 8030X Y MeAH 3030X Y 24 24 525 000 Human Lung Cancer MeAH 041X Y _ MeAH 8040X Y MeAH 3040X Y E G 4 4 252 000 Human Prostate Cancer MeAH 051X Y _ MeAH 8050X Y MeAH 3050X Y 96 ANGC_D FE II2X4 252 000 Human Stem Cell Transcription Factors MeAH 511X Y E G 2 2 168 000 Human Inflammatory Response MeAH 521X Y 12 12 525 000 Human T Cell Activation MeAH 531X Y 24 24 840 000 Human Cytokine Production MeAH 541
53. ADD 20960FF RCAS 009 8 1 10 31 100 000 HRCAS 60 000 9 32 Cosrmo Bio News No 76 2009 ELISA KIT 2009 7 1 10 30 ELISA ELISA KIT GENERAL 7 4 550 68 250 ELISA KIT pink ONE 95 550 73 500 ADD 2009 AbD 14 000
54. Cosrmo Bio News No 76 29 ELISA 11 IFN cg ELISA VeriKine HS WW Cynomolgus Rhesus IFN c Serum ELISA Kit IFN 5pg 8 1502 96CV MB 25pg m2 IFN ca 25 1 600pg m2 1 X96 896 1 IFNg IFN PBL InterferonSource PBL ei VeriKine HS
55. 5 FAM Ex Em 4980nm 520nm 5 FAM SensoLytes 550 FRET ANAGPEC 9 96 100 1 5 FAM QXLTM 520 Ex Em 490nm 520nm MM5 FAM Ex Em 490nm 520nm 2 lt Anaspec SensoLyte 520 Thrombin Activity Assay Kit 79 000 Cosrmo Bio News No 76 2 505
56. EDNA 2 DNA DNA 85818 Cosmo_Bio would like to acknowledge and thank SABiosciences Corporation for providing PATHWAYS ISSUE 8 2008 http WWW SaDiosclences COm presented here 1 Bird A Nature 447 396 98 2007 Bradley E et al Ce 128 669 81 2007 3 0zanne SE Const84ncia M Nat Clin Pract Endocrinol Metab 3 539 46 2007 4 Feinberg A and Tycko B Nat Rev Cancer 4 143 53 2004 5 Esteller M N Engl J Med 358 1148 59 2008 6 The AACR Human Epigenome Task Force amp EUNE Scientific Advisory Board
57. DAPI Degrees of Labeling dyerproteln 4 555 A C IgG s Biotium Inc BTI Abs Em nm CF 488 succinimidyl ester 488 516 Amine reactive 92120 1 umol 66 000 CF 555 succinimidyl ester 552 574 Amine reactive 92130 1 umol 66 000 CF 647 succinimidyl ester 650 674 Amine reactive 92135 1 umol 46 66 000 CF 647 hydrazide 650 674 Aldehyde or ketone reactive 92136 1 47 000 CF 660 succinimidyl ester 664 685 Amine reactive 92137 1 mol 666 000 CF 680 succinimidyl ester 679 701 Amine reactive 92139 1 umol 70 000 CF 750 succinimidyl ester 752 786 Amine reactive 92142 1 umol 70 000 CF 770 succinimidyl ester 766 800 Amine reactive 92150 1 mol 70 000 CFrM dye CFrW dye Abs EM VivoBrite rapid antiDody labeling kit x XPBS pH 4 N 10K MWCO 5 SE 96160 CF750 SE 92161 W CF770 SE 9616 DMSO _
58. DNA 5 2 CpG 5 5 m5C DNA m5C DNMT1 DNMT3a DNMTG3b93 1 DNA DNMTs DNMT3a DNMTGb ge novo CpG De novo early ES 05 DNA DNMT 1 CpG DNA 9 19 DNMTs DNA 0 DNMTs
59. Ready to use NutriStem with HSA Human Serum Albumin NutriStem without HSA Human Serum Albumin hN2TM FACS DNA RT PCR FLIPR hN2rM Human Neurons hESC WAO0g8 Matrigelr 14
60. 1 RCAS B 00 2 SEN SN 2 2 2 2 32 1 2 2 2 1 1 1 2 1 1 221 2 1 CO 158 oO7T 19S 2 2 2 2 2 a Sg TRo 2 2 2 2 2 2 2 2 RXR a 2 2 2 2 2 2 RXRy 2 32 2 2 OD
61. DNA 5 1 DNA CpG CpDG CpGls 5 CpG 50 7096 CpGls X 5 79 CpDG 8 DNA 1 DNA CpGls
62. PPAR 8 ER WTMB MR VDR ER VDR DMSO ER 60 ID TR 10 RT 7 RXR PPARo PPARHY PPAR68 VDR TRo TRj LXR LXRg FXR RXR II 70C A 1 EnBio RCAS for ER Ts 1 kit 100 000 60 000 EnBio RCAS for ER B ERB SRC 1 kit 100 000 60 000 2 EnBio RCAS for PPAR a PPARA CBP 1 kit 100 000 60 000 EnBio RCAS for PPAR y PPARG CBP 1 kit 100 000 60 000 EnBio RCAS for PPAR 8 PPARD CBP 1 kit 100 000 60 000
63. 22 2 lt lt 3 1 ER PPAR y PPAR 8 VDR 1 PPAR PPAR y PPAR 8 FXR 36 PPAR oc PPAR y PPAR 8 ER PPAR co PPAR y LXR PPAR y VDR 7872 PPAR 5
64. DSB 4m6 DNA Methylation Detection Kit 6 Cosrmo Bio News No 76 TE 1 5mg Met 5 M CMP 100 DNA 2 g 100 50 1 MW 100bp 1 U PCH DNA U PCH DNA 3 M PCR DNA 4 M PCH DNA Unmethylated DNA GGGCGGGGCGGACCGG Sodium bisulfit GGGUGGGGUGGAUUGG Methylated DNA Sodium bisulfit G BioChain Institute Inc BCH K5082100 1 kit 59 200 s gt amp Sant 8 11
65. 15 13W 13 15 10 150K 443152 5 9 800 2 15 17W 17 443169 5 4 9 800 DCB 1 oo m EE II 6 9 13W 6 9 45 250K 250 2 000 414992 9 800 1 6 9 17W 415005 9 800 3 IP 9711 13W 9 11 30 200K 140 1 700 415012 6 9 800 9711 17W 415029 5 9 800 IP 11714 13W 13 11 1496 20 150 60 1 500 415036 5 9 800 3 1 11714 17W 17 415043 5 9 800 1 14 16 13W 13 14 1696 15 100 40 1 200 415050 5 9 800 IP 14 16 17W 17 415067 5 9 800 2 2 257 69 6 5KDa 4 74 W x14 L mm 28
66. 16 ast Diges SPCHR DNA DNA FastDigest Miniprep DNA 40 72 6 a DNA 60 DNA 40 17 mMPCR DNA DNA MRHFLP 146 432 3 Fermentas AB FER Fermentas AB FER 14061 FD0944 20 pl 11 000 Kpn2I FD0534 100 pk 16 000 Afel Eco47Ill FD0324 20 pl 28 000 Kpnl FD0524 300 13 000 Agel BshTI FD1464 20 pl 15 000 Mbol FD0814 50 pl 20 000 _ Alw211 FD0024 100 18 000 Mboll FD0824 50 pl 14 000 AlwNI Cail FD1394 50 pl 15 000 Mfel Munl FD0753 20 pl 8 000 Apal FD1414 300 pl 10 000 MIul FD0564 100 pk 11 000 Ascl Sgsl FD1894 100 18 000 Mnll FD1074 50 20 000 Aval Eco881 FD0384 200 pl 16 000 Mscl Mlsl FD1214 50 pl 20 000 Avall Eco
67. POSTIVE NEGATIVE INVALID S Z E U INMUNOTEC PROTEON Gluten EXPRESS 25 Y54 000 PROTEON OVO EXPRESS ZE PR O25 25 test 54 000 PROTEON B Lactoglobulin EXPRESS ZE PR L25 25 test 54 000 4 Le 4 amp boom AxyVacrM SBS 96 384 ABS 96
68. 70O 196C N Phire PCR A FINN YE5 PCR 5 PhirerM Plant Dierct PCR Kit Phirem DNA PCR W2xPhire PCH dNTPs MgCls Whatman 903 FTAe Phire DNA Harris Uni Core DNA Harris Cutting Mat DNA DNA Fidelit
69. Cell Biolabs Inc miRNASelect V pDEGP mir Cloning and Expression Vector MIR EXP GP C 100 pk 123 000 miRNASelectTV pEP mir Cloning and Expression Vector MIR EXP C 100 pk 124 000 miRNASelect V pDEGP mir Null Control Vector MIR NULL GP 100 pk 92 000 2 miRNASelectTV pEP mir Null Control Vector MIR NULL 100 pk 92 000 Cosrmo Bio News No 76 Ne X x NEW PowerCut Dicer amp Replicator Ja RNAi FINNZYMES PowerCut Dicer 22222252 222222 PowerCut M Dicer dsRHNA 25 7 siRNA 67 g7 g intestinalis 100 siRNA siRHNA siRNA
70. IP 5710 17W 17 443114 5 9 800 2 16 13W 13 8 16 20 200K 70 1 500 417269 5 9 800 2 1 8 16 17W 17 417276 5 9 800 10 20 13W 13 10 20 12 130K 30 1 500 414893 5 9 800 2 1 10 20 171 17 414909 5 9 800 2 2D 10 20 1 4 0 2099 12 130K 415074 5 9 800 2 1 15 20 13W 13 15 20 3 85K 3 432026 5 9 800 2 15 20 17W 17 443121 5 9 800 2 15 25 13W 13 15 25 3 85K 3 20 1 000 414916 5 9 800 2 1 15 25 1711 17 414923 5 9 800 2 DCB 5 13W 5 100 500K 443138 9 800 2 5 17W 443145 9 800 2 IP 7 5 13W a 7 5 45 250K 250 2 000 414930 9 800 2 7 5 17W 414947 5 9 800 2 10 13W 13 10 30 200K 140 1 700 414954 5 9 800 2 10 17W 17 414961 5 9 800 2 12 5 13W 13 12 5 20 150K 60 1 500 414978 5 9 800 2 12 5 17W 17 414985 5 9 800
71. 10 23 12 15 183 15 2009 8 Biosciences Inc at sje AAAS 009 Sienaling A 3 Science Signaling AAAS Science AAAS AAAS Se 5c ejce Signaling 8 One _
72. NH PBS 1004u2 rs b 3 NH O157 8001 25 test 8 000 NH O26 B00026 25 test 8 000 2 NH O111 800111 25 test 8 000 22 NH 0O157 999100000 1 kit 20 tes1 10 000 NH 0O26 999400000 1 kit 20 tes1 10 000 NH O111 999500000 1 kit 20 tes1 10 000 NH 999200000 1 kit 20 test 14 000 NH 999300000 1 kit 20 test 10 000 NH 999600000 1 kit 20 test 14 000 5 Cosrmo Bio News No 76 2 205 DNA RNA RNA DNA THT RNA
73. 1 Methy Profiler PCR amp ChampionChIPT MethyIMagnet W mCpG DNA MethyIMagnetrV mCpG Ne DNA ICM DNA a September 2009 2 lt CF Dyes ALADO 7 1 7 N DNA MethylL Profiler PCR amp 2 ChampionChIP 3 MethyIMagnet DNA 4 MethyIMagnet mCpG 5 DNA ICM 6 DNA 6 AGEs ELISA 10 NutriStem ES 12 PROTEON EXPRESS 15 CF Dyes 23 25 ALADO 26 TrueORF
74. GST MBD MethyIMagnetrM MethyIMagnet w DNA S GST MBD2 MBD MethIMagnetrw DNA MBD2b CpG CpG CpG MBD2 CpG mCpG DNA
75. eGFP siRNA 1nM eGFP siRNA 10nM HeLa pEGFP C 1 HeLa eDGF siRNA phi6 S PowerCutw Dicer 6GFP siRNA SIHNA eGFP B C siRNA A eGFP siRNA 2 Replicator RNAi dsRNA Finnzymes Oy FZM PowerCut M Dicer F 300 55 000 F 602S 60 unit 13 000 Replicator RNAi cocoooeoooeoooooooeeooooooooeeeoooooooeooeeoeoooooooeeooooooooeeeoooooooooeeoooooooeeeeeooo dsRNA O RNA RNA RNA
76. 20 50 2x10 mg 10 2x 5x 2x 20 2x 5x 2x 4x 10x 6x PuRetroTV 2x 5x 2x PuRetro Applied Biological Materials Inc APB Add N PureTM Adenovirus Purification Kits Giga 2xGiga Purification Sets A910 1 kit 62 000 Add N Pure M Adenovirus Purification Kits Maxi 5x Purification Sets A905 1 kit 82 000 Add N PureTM Adenovirus Purification Kits Maxi 2x Purification Sets A902 1 kit 43 000 PuRetroTM Lentivirus and Retrovirus Purification Kits Giga 2xGiga Purification Sets G173 1 kit 62 000 PuRetro Lentivirus and Retrovirus Purification Kits Maxi 5x Purification Sets G172 1 kit 82 000 PuRetro Lentivirus and Retrovirus Purification Maxi 2x Purification Sets G171 1 kit 43 000 TEL 03 5632 9610 FAX 03 5632 9619 20 Cosrmo Bio News No 76 FastDigest 1 FastDigest Unit FDU 1 FastDigest 1 g 4 DNA FastDigest 1 FDU 2 Cosrmo Bio News No 76 21 MW
77. ShRNA pRS EGFP pDGFP V RS pRFP C RHS turDoGFP turDboRFP pGFP V RS pRFP C RS 221 222 mm ei tt Rh LT 11 ram TAD TY TrHgr 5522 FLIR Fj he i PFET ET CIT 1 Scrambled shRNA GFP Vector Scrambled shRNA RFP Vector shRNA against RFP GFP Veotor shRNA against GFP RFP Vector 16 Cosrmo Bio News No 76 HUS
78. amp 15 000 MAB PAB KA 30 OFF LipOoSEARCH 009 9 1 12 26 LipOoSEARCH TC amp TG 40008 12 00 0 10 000 7 200 TCS amp TG 11 200 9 800 AbD G0960FF 009 7 81 10 30 CD
79. 1 M pH8 3 4mm 0 2um Biotium Inc BTI Abs Em nm VivoBrite rapid antibody labeling kit for in vivo imaging CF680 SE 679 701 Amine reactive 92160 1 kit 123 000 VivoBrite rapid antibody labeling kit for in vivo imaging CF750 SE 752 786 Amine reactive 92161 1 kit 123 000 VivoBrite rapid antibody labeling kit for in vivo imaging CF770 SE 766 800 Amine reactive 92162 1 kit 123 000 Cosrmo Bio News No 76 23 1 4 HydraLinKTM 100 HRP ELISA NEW UniversalrM 4 5 60 6 10 40
80. Dyes 0 seconds_ 10 seconds 5 CF in V mpH 1 25672228 9 5 3 HeLa A CF 666 lgG o HeLa Image Pro ExpresS 0 10 4000 gt EE 7 1 HeLa CF 555 CF 488 IgG
81. 150 pk 100 Lug 100 Lg 100 Lug 150 pk 150 100 Lug 150 pk 0 1 0 1 mg 150 pk 100 Lg 0 1 mg 100 ug 150 pk 150 pk 0 02 mg 150 0 1 150 100 Lg 0 1 mg 150 pk 0 1 mg 100 Lg 468 000 468 000 776 000 468 000 468 000 468 000 468 000 466 000 468 000 468 000 725 000 468 000 744 000 776 000 852 000 770 000 852 000 468 000 775 000 833 000 468 000 468 000 466 000 468 000 455 000 468 000 452 000 452 000 468 000 468 000 468 000 775 000 452 000 740 000 468 000 468 000 462 000 468 000 726 000 468 000 468 000 744 000 466 000 148 000 468 000 468 000 468 000 468 000 726 000 468 000 443 000 466 000 468 000 442 000 852 000 150 0 1 mg 100 pk 150 100 Lug 468 000 742 000 460 000 468 000 748 000 150 150 0 1 mg 150 150 100 Lg 100 Lg 0 1 mg 0 1 mg 150 pk 25 150 150 50 Lg 150 468 000 468 000 466 000 468 000 468 000 432 000 740 000 466 000 466 000 468 000 814 000 468 000 468 000 851 000 468 000 70 196 0 1g 66 000 PARS2 AP7782C 0 1 742 000 NUF2 PGI
82. 20 mk 50 mg 20 mg 10 05222 22 5x 2x 5 20 2x 5x 2x 221155 4x 10x 6x Add N Pure VM 2x 5x 2x Add N PurerM PuRetroW Lentivirus and Retrovirus Purification Kits AB05 A905 50W AB 10 150M PuRetrorV Maxi 2x Giga 2x G171 G172 G173 10 mg 25 20 20 md 50 ml 20 ml
83. 65700 TOPICS x RNA DNA 10 100 m2 20 mg 600 pk 100 pl rRNA miRNA RNA 45 ji DNase DNA 0 RNA 50 LE ORR 0 22um 0 45um 30 mg 25 15 mg 8 30 mg 15 mg 5023 A a DNA
84. Hooke LaDboratorieS InC ES PLP139 151 CFA II 8 10 Pr 20 10 102 000 PLP139 151 CFA Emulsion PTX SJL 8 10 EK 0121 10 test 109 000 PTX Hooke Laboratories Inc MOGss ss CFA Emulsion C57BL 6 8 10 11 10 22 667 000 PLPi139 151 CFA Emulsion SJL 8 10 EK 0122 10 test 67 000 2 Ovalbumin CFA Emulsion any any EK 0301 10 test 67 000 CIA Hooke Laboratories Inc 2 Chicken Collagen CFA Emulsion 0 20 oot 109 000 I Chicken Collagen IFA Emulsion DBA 1 7 EK 0211 20 test 102 000 I Bovine Collagen CFA Emulsion DBA 1 7 10 EK 0220 20 test 109 000 I Bovine Collagen IFA Emulsion DBA 1 7 0 EK 0221 20 test 102 000 225471 M
85. SPA 2 7 H3 ChampionChIP M Antibody Kits for Human H3ac Lys9 14 GAH 7201 1 kit 12 73 000 ChampionChIPTM Antibody Kits for Human H3K9ac Lys9 GAH 1209 1kit 12 73 000 ChampionChIP Antibody Kits for Human H3K4me1 Lys4 GAH 5207 1 kit 12 73 000 2 ChampionChIPTV Antibody Kits for Human H3K4me2 Lys4 GAH 3203 1 kit 12 73 000 ChampionChIP M Antibody Kits for Human H3K4me3 Lys4 GAH 8208 1 kit 12 73 000 ChampionChIP M Antibody Kits for Human H3K9me1 Lys9 GAH 5210 1 kit 12 73 000 ChampionChIPTV Antibody Kits for Human H3K9me2 Lys9 GAH 8211 1 kit 12 73 000 ChampionChIPTV Antibody Kits for Human H3K9me3 Lys9 GAH 6204 1 kit 12 73 000 2 ChampionChIP M Antibody Kits for Human H3K27me3 Lys27 GAH 9205 1 kit 12 73 000 ChampionChIPTM Antibody Kits for Human H3K36me3 Lys36 GAH 1212 1 kit 12 73 000 ChampionChIPTV Antibody Kits for Human H3K79me3 Lys79 GAH 4213 1 kit 12 73 000 SR ChampionChIPTV Antibody Kits for Human H3 GAH 2
86. CD oe 2008 010 Fes Ti 1 HINET 2008 20092010 S010 epi HSP 2008 2 010 009 2010 EIA
87. EnBio RCAS for LXRc LXRA SRC 1 kit 100 000 60 000 EnBio RCAS for VDR VDR SRC 1 kit 100 000 60 000 EnBio RCAS for MR MR SRC 1 kit 100 000 60 000 EnBio RCAS for TR a TRA SRC 1 kit 100 000 60 000 EnBio RCAS for TR B TRB SRC 1 kit 100 000 60 000 EnBio RCAS for RXR a RXRA SRC 1 kit 100 000 60 000 EnBio RCAS for RXR B RXRB SRC 1 kit 100 000 60 000 EnBio RCAS for RXRy RXRG SRC 1 kit 100 000 60 000 EnBio RCAS for FXR FXR CBP 1 kit 100 000 60 000 Cosrmo Bio News No 76 9 ii 505 AGEs ELISA AGEs ELISA rseenshe AGEs advanced glycation end products AGEs AGEs
88. IFN o Serum ELISA Kit 461 1 1x96 well 176 000 IFN ELISA IFN o IFN IFN 8 IFN 8 1a IFN8 1D VeriKine HS Human IFN 8 Serum ELISA Kit 6 1096 MHC NK IFN 8 MS Avonex Biogen Rebiff EMD Serono Betaseron Bayer Extavia Novartis IFN 8 IFN 9 5pg m 2 IFN 8 0Q 3 150pg m2g IFN ca IFN y
89. 1 NA XA 2 x hESC NutriStemrMw hESC XF hESC MatrigelrW hESC rh bFGF rh TGF hESC hESC NutriStemrV with HSA 05 100 1A 05 100 1B NutriStemrM without HSA 05 102 1A 05 10Z 1B
90. 15731 1 AP 150 0 68 000 PD2R BWT BS3239 100 152 000 NVL ABG AT3139A 0 1g 66 000 PDCD12 ATG APD0962 50 0 123 000 NXPH1 PGI 13809 1 AP 150 68000 PDZ11 ABG AP7791A 0 1g 742 000 PGC1 B RSD AF5656 100 ug 775 000 69 000 PMP70 BET A301 998A 0 17g 68 000 150 pk 468 000 PPFIBP1 13961 1 AP 150 pk 468 000 PGI 15212 1 BET AS302 035A ABG AT3167A BET A301 978A 68 000 14896 1 AP 468 000 0 1 mg 0 1 mg 466 000 468 000 PA1 53 72 6 1 7 7 5 NN 0 0 Ut OH WO WY co 0 NO2 6 SH SH WT Sv ATPyS 1 2686 1 51 8 Allen and Shokat Nature Methods 2007
91. IC50 984 7nM Cosrmo Bio News No 76 25 2 1 X 1 NEW ALADO _ W ALADO
92. p53 ChIP PCR A549 HepG PC3 5 FU 300M 6 p53 CDKN 1A p53 qPCR HRNA RT PCH CDKN1A CDKN1A p53 A549 HepG DNA p53 PC3 SABioscience Corporation SPA SPA 1 ChampionChIPTM ChIP Target Specific Kits for Human RNA Polymerasell RNA II GA 111 1 kit 12 73 000 ChampionChIPTM ChIP Target Specific Kits for Human p53 p53 GA 112 1 kit 12 73 000 SABioscience Corporation
93. 100 AB2 Basal Medium ANS Supplement 4867 M60100 100 AB2 Basal Medium 2m ANSTM Supplement hN Humman eurons G Tuj 1 Neuromics Antibodies Inc NUR HN60000 12106 cells 232 000 M60100 1 set 25 000 dd 4C 20C 7OC 196C 0157 026 01 1 1 O0157 O26 O11 1 NEW 0157 026 01 1 1 0157 086 01 1 1 25
94. 25 100 Lg 150 pk 150 100 0 1 mg 100 Lg 100 pk 0 1 mg 150 150 100 Lg 0 1 mg 0 1 mg 0 1 mg 150 pk 100 Lg 100 Lg 0 1 mg 150 100 pk 150 0 02 mg 100 Lg 0 1 mg 100 Lg 150 pk 100 pk 100 Lg 150 100 Lg 150 pk 0 1 mg 0 1 mg 150 150 pk 100 g 150 pk 468 000 442 000 468 000 466 000 452 000 452 000 776 000 468 000 466 000 468 000 466 000 468 000 468 000 448 000 468 000 468 000 466 000 466 000 466 000 468 000 466 000 466 000 444 000 830 000 468 000 466 000 466 000 452 000 468 000 468 000 452 000 452 000 452 000 468 000 830 000 468 000 466 000 466 000 468 000 452 000 468 000 462 000 452 000 814 000 452 000 468 000 468 000 460 000 466 000 452 000 830 000 769 000 468 000 468 000 748 000 442 000 442 000 442 000 468 000 444 000 452 000 442 000 468 000 720 000 468 000 468 000 774 000 466 000 452 000 468 000 830 000 740 000 468 000 452 000 468 000 466 000 466 000 468 000 468 000 452 000 468 000 CPT1C PGI 12969 1 AP D D2HGDH PGI 13895 1 AP DAT APB 050529 DBNL PGI 13015 1 AP DCLRE1B PGI 13203 1 AP DCT PGI 13095 1 AP DEFB1 PGI 14738 1 DFNB31 ABG AT1757A DHDDS PGI 15099 1 AP DHRS4 PGI 15279 1 AP Dihydroepiandrosterone 11 a FKA 108 DIRAS1 PGI 12634 1 AP E2F4 E2F5 BWT BS1095 EAPII APB 052393 Elongin A1 BWT BS2530 ENaC y APB 059799 ENaC 6 BWT BS3070 ENPP 5 PGI 12778 1 AP ER oc NR3A1
95. 40u2 Buffer 8 with Aniline 2x1 5mg Ready to use HRP Bowne pHRP Canjugate Mee reHTCHRP Coniugate 5 UV MS HyNic Linker 2x100Lug Buffer 2 1 5 HRP Antibody All in One Conjugation Kit 4FB modified HRP 5 UniversalrM DAB Substrate Kit MEnhancer 100 NM50xWB Probe R50 2 10xRapid Wash Solution 100mg 100xDAB Solution Set 2mg SuperHi Chemiluminescence Kit Enhancer 100mg 50xWB Probe R51 0xRapid Wash 1 SuperHi Chemiluminescence 40
96. Assay K 1000S 1 kit 96well 111 000 8 10 23 p Epia ELISA gt ELISA ELISA TMB B 1 gt HabMAB 1 2 96wel HRP 100X 0 1 ELISA 10X TMB A 1 X 1 ELISA EPITOMICS INC EPT Total amp Phospho pT91 93 c Jun Dual Detection Elisa Kit 6116 1 1 kit 96 test 95 000 Total amp Phospho pY492 ZAP 70 Dual Detection Elisa Kit 6117 1 1 kit 96 test 95 000 c Jun phospho Tyr91 93 ELISA Kit 6115 1 1 kit 96 test 95 000 ZAP 70 Total ELISA Kit 6114 1 1 k
97. Mgrure 454 71 1 15 2008 7 Jones A and Baylin B Ce 128 682 92 2007 8 Laird P Nat Rev Cancer 3 253 66 2003 9 Callinan and Feinberg A P Hum Mol Genet 15 Spec No 1 R95 101 2006 10 Groth A et al Ce 128 721 33 2007 1 1 Muegge K Biochem Cell Biol 83 548 549 2005 1 Huang J et Nucleic Acids Res 32 5019 28 2004 13 Chen and Li E Curr Top Dev Biol 60 55 89 2004 14 Bestor Hum Mol Genet 9 2395 402 2000 15 Okano M et al Ce 99 247 57 1999 16 LiE gg Ce 69 915 26 1992 17 Fatermi M et al Nucleic Acids Res 33 e 1 76 2005 18 Meissner A et al Nature 454 766 70 2008 19 Ohm J E et al Nat Genet 39 237 42 2007 20 Widschwendter M et al Nat Genet 39 157 58 2007 Cosmo Bio News No 76 A XA 3 N A Ne 205 DNA MethyLHProfiler VW PCR amp DNA SABiosciences 1 DNA Methyl ProfilerrM PCH
98. News No 76 3 3 N A x DNA MethylIMagnet mCpG DNA 2 DNA MethyIMagnet rM GST MBD dsDNA 1 ng 1 ug DNA mCpG DNA 6 CpG DNA DNA 1 Ss TE GST MBD HeLa DNA
99. Pure v Adenovirus Purification 48 2 109 10 3pfu AB02 Ad05 101 10 pfu m2g A910 10 10 3pfu NEW DI Add N PureTV 22 824 524 3 A902 A905 A910 10 mg 25 mk 30 mg 1 487 DL 09 10 mg 25 ml 20 80 22100 80
100. Uni CoreTM amp Cutting Mat 52 PCR 2 PCR 9 76 9 6 NaCIO PCR PCH Finnzymes Oy FZM Harris Uni Core M 0 35 mm F 180S 5 pc 9 000 F 180L 25 pc 41 000 Harris Uni Core 0 50 mm F 185S 5 pc 9 000 F 185L 25 pc 41 000 Harris Cutting Mat 6 4x7 6 cm F 190S 5 pc 5 000 9 Cosrmo Bio News No 76 9 2 Add N
101. mCpG DNA C RiboMed IONBENI9I9gI6 Inc RMB MM1 K 25 675 000 MethyIMagnetTV mCpG DNA MM101 K 2009 9 30 5 MR22 01 4 Cosrmo Bio News No 76 MethyIMagnetTV Proteins MethyIMagnet Proteins 1 5m2 8 0 6 6 PCRH 8 4C 0C 70C 196C RiboMed i uic ed Inc Eo 3 ME ET MM101 100 Lg 68 000 1 22 SM Diolec nsogies Inc EE EE 22 Tube Magnetic Separation Rack MethyIMagnet V mCpDG DNA CpG qPCR MS PCH
102. mCpG Island Primer Kit TFAP2A MethyIMagnet V mCpG Island Primer Kit TMEFF2 MethyIMagnet V mCpG Island Primer Kit TWIST MethyIMagnet V mCpG Island Primer Kit Custom Any combination of islands in sets of 8 reactions Choose 12 01 1 WPS 57 000 Unmethylated Methylated Input DNA DNA DNA S Supernatant fraction E Eluted fraction P16 Amplicon Minus DNA 1 HeLa DNA 1ng MethyIMagnet Vw mCpG DNA MM101 K HeLa DNA 2ug6 MethyIMagnet rV mCpG p16 Island p16 SYBR green Typhoon MethyIMagnetrV mCpG DNA DNA DNA PPCR 40 DNA Sa hn Inc RMB MM201 rxn 60 000 MM201 BRCA1 96 rxn 60 000 MM201 CCNA1 96 rxn 60 000 MM201 CDKN2A 96 rxn 60 000 MM201 DAPK1 96 rxn 60 000 M
103. 206 1 kit 12 73 000 H4 ChampionChIP V Antibody Kits for Human H4ac Lys5 8 12 16 GAH 0202 1 kit 12 73 000 ChampionChIPTM Antibody Kits for Human H4K20me3 Lys20 GAH 7214 1 kit 12 73 000 ChampionChIPTV One Day ChIP_ DNA PCH DNA ChIP DNA amp SABioscience Corporation SPA ChampionChIPTM ChIP Preparation Kit GA 101 1 kit 12 52 000 ChampionChIP PCER DNA Corporation A ChampionChIPT Primer GA D m A 42 000 PCR sabiosciences cosmobio co jp Cosmo Bio
104. 47I FD0314 200 pl 16 000 Msill Rsel FD2004 20 pl 17 000 Avril Xmaydl FD1564 20 pl 30 000 Mspl FD0544 400 13 000 BamHI FD0054 800 pl 10 000 Mssl FD1344 100 pk 20 000 BanI BshNI FD1004 300 pb 11 000 1269 FD0964 50 20 000 BbsI Bpil FD1014 20 pl 11 000 Mval FD0554 200 10 000 21 Bfal FspBI FD1764 50 pl 20 000 Ncil Benl FD0064 200 16 000 Bgll FD0074 200 14 000 Ncol FD0573 20 pl 8 000 Bglll FD0083 100 pk 10 000 Ndel FD0583 100 pk 11 000 BlpI Bpu11021 FD0094 50 pl 20 000 NlalV BspLI FD1154 20 pl 13 000 BsaHl Hin11 FD0474 100 pk 15 000 NmuCl FD1514 20 pl 18 000 BsaJl BseDI FD1084 20 pl 10 000 Notl FD0593 20 pl 8 000 BseNI FD0884 100 18 000 Nsil Mph11031 FD0734 100 pk 14 000 Bsll BseLI FD1204 100 pk 20 000 Pdml FD1534 100 18 000 Bsp1191 FD0124 200 pl 9 000 PfIMI Van911 FD0714 100 pk 18 000 Bsp1407 FD0933 50 pl 10 000 Pfol FD1754 20 pl 35 000 BsrFI 101 FD0184 50 pl 19 000 PpuMI Psp5II FD0764 50 pl 18 000 BstXI FD1024 100 pk 15 000 PshAI 8024 FD1434 100 pk 20 000 BstZ17I Bst1107I FD0704 100 pk 19 000 Pstl FD0614 800 14 000 Clal 8 151 FD0143 50 pl 8 000 Pvul FD0624 20 pl 18 000 Csp6I FD0214 100 pl 10 000 Pvull FD0634 400 pl 13 000 Ddel HpyF3I FD1884 50 pl 15 000 Rsal FD1124 100 pk 13 000 Dpnl FD1703 50 pl 9 000 Sacl FD1133 100 pk 9 000 Dral FD0224 200 pl 10 000 Sall FD0644 200 13 000 Dralll
105. 6 27 28 ELISA 30 3 AS 32 http why mods jp
106. 8 EnBio RCAS 9 AGEs ELISA 10 10 INNO LiPA Apo 11 SensoLyte 520 11 NutriStemTM ES 12 hN2TM 12 215 117 O157 O26 O111 13 DNA RNA 14 PROTEON EXPRESS 15 AxyVacrM 15 HuSH 29 Pre designed shRNA 16 miRNASelectTM miRNA 17 PowerCut Dicer amp ReplicatorTM RNAi 18 Phire PCR 19 20 FastDigest 21 II 22 CF Dyes 23 HRP Antibody 24 UniversalrV 24 25 ALADO 2
107. Adel FD1234 50 pl 11 000 Sapl Lgul FD1934 20 20 000 Eam1105I FD0244 100 pk 19 000 Sau96I Cfr13I FD0194 100 pk 13 000 11361 FD0254 100 pk 10 000 Sdal FD1194 20 pl 13 000 311 FD0293 50 pl 10 000 ScrFI Bme1390 FD1424 100 pk 19 000 Eco91I FD0394 200 pl 14 000 Sfcl Bfml FD1164 20 pl 15 000 EcoNI Xagl FD1304 100 pk 15 000 Smal FD0663 100 pk 8 000 EcoRI FD0274 800 pl 10 000 SnaBI Eco105I FD0404 50 pl 17 000 EcoRV Eco32 FD0303 200 pl 10 000 Spel Bcul FD1253 20 8 000 Ehel FD0443 20 pl 10 000 Sphl Pael FD0604 20 pl 10 000 Fnu4HI Satl FD1644 20 pl 16 000 FD0774 100 pk 19 000 4 FspAI FD1664 20 pl 24 000 Stul Eco1471 FD0424 100 pk 10 000 Fspl FD1224 50 pl 16 000 Styl Eco1301 FD0414 200 10 000 Haelll BsuRI FD0154 400 pl 10 000 Swal Smil FD1244 200 20 000 Hhal FD1854 200 pl 15 000 Taal FD1364 20 pl 14 000 Hincll FD0494 100 pk 16 000 Tail FD1144 50 18 000 HindIl FD0504 800 pl 10 000 Taql FD0674 400 13 000 Hinfl FD0804 400 pk 15 000 Tru1l FD0984 50 pk 18 000 HinP1I Hin6 FD0484 200 pl 14 000 Xapl FD1383 50 pl 11 000 Hpal KspAI FD1034 50 pl 9 000 Xbal FD0684 300 11 000 Hpall FD0514 200 pl 11 000 Xhol FD0694 400 10 000 60C
108. BG BWT APB PGI ABG ABG BWT PGI PGI BWT BWT BWT PGI APB PGI ABG ABG PGI BWT PGI EVR BWT BMO BWT PGI PGI APB ABG BWT APB PSC PGI PGI BWT ABG ABG ABG PGI BWT BWT ABG PGI APB PGI BET ALP ABG BWT PGI APB EVR PGI BWT PGI ABG ABG PGI PGI BWT PGI 14900 1 AP AP2779C A301 999A AT1009A BS4209 BS1377 Y050475 15421 1 AP AT1026A 12965 1 AP AT1030A 14221 1 AP 13822 1 AP BS1988 14640 1 AP 14361 1 AP AT1135A AT1142A AT1145A 14553 1 AP AT1152A AT1153A BS1016 Y058068 14349 1 AP AT1192A AT1194A BS2213 12328 1 AP 14116 1 AP BS1840 BS1613 BS1841 13399 1 AP Y058033 12421 2 AP AT1276A AT1279A 14324 1 AP BS4024 14956 1 AP EB05868 BS1035 PW0570 0025 BS3092 13461 2 AP 14298 1 AP Y072001 AT1314A BS4217 Y058154 45 351 13729 1 AP 14479 1 AP BS2052 AP7768A AP7777C AP7846B 14311 1 AP BS1040 BS1042 AP7767A 12963 1 AP Y052751 11728 1 AP A301 969A CSPV11 M AT1554A BS7001 13938 1 AP Y058079 EB08923 14337 1 8 2184 13548 1 AP AT1587A AT1590A 14749 1 AP 13604 1 AP BS3278 13385 1 AP 150 0 1 mg 0 02 mg 0 1 mg 100 Lg 100 Lg 100 Lg 150 0 1 mg 150 pk 0 1 mg 150 pk 150 100 Lg 150 150 0 1 mg 0 1 mg 0 1 mg 150 0 1 mg 0 1 mg 100 Lg 100 pk 150 pk 0 1 mg 0 1 mg 100 Lg 150 150 pk 100 Lg 100 Lg 100 Lg 150 100 pk 150 0 1 mg 0 1 mg 150 100 Lg 150 pk 100 Lg 100 Lg
109. C cDNA FAQ http www cosmobio co jp p roduct products_ORG _ 0090624 asp MR MM Inc ORG FAK N VCL N ZYX C B4GalT1 C TGOLN C LAMP1 C BDHA1 C BID C GAP43 C CCND1 C LMNB1 N PXMP2 N LCK C SYP N RC100012 RC100031 RC100032 RC100005 RC100029 RC100016 RC100006 RC100007 RC100013 RC100009 RC100018 RC100024 RC100017 RC100028 RC100044 RC100063 RC100064 RC100037 RC100061 RC100048 RC100038 RC100039 RC100045 RC100041 RC100050 RC100056 RC100049 RC100060 0 dd 4C G0C 70C 196 EnBio RCAS RCAS
110. CR mae ne conj control 112 378 112 5 158 1 158 4 INNOGENETICS N V 8561 INNO LiPA Apo 80393 80294 1 kit 20 test 133 000 ApoE 2 g 4 NEW SensoLyte 520 FRET bB FAM QXL FRE
111. H 29 Pre designed shRNA ORIGENE TECHNOLOGIES INC HuSH 29 mer 59mer ShRNA GO 55 30 70 GO 3 5 Iocus ID 3 pRS pDGFP V RS GFP DRFP C RS pGFP V RS RFP pRS mm 5 g 4 ShRNA 70 4 1 70
112. M201 DKKS 96 rxn 60 000 MM201 ESR1 96 rxn 60 000 MM201 FAM127A 96 rxn 60 000 MM201 FHIT 96 rxn 60 000 MM201 GAPDH 96 rxn 60 000 MM201 GSTM1 96 rxn 60 000 MM201 GSTP1 96 rxn 60 000 MM201 hMLH1 96 rxn 60 000 MM201 HOXA2 96 rxn 60 000 MM201 MCAM 96 rxn 60 000 MM201 MGMT 96 rxn 60 000 MM201 MLH1 96 rxn 60 000 MM201 PAKS3 96 rxn 60 000 MM201 PR 96 rxn 60 000 MM201 PTGS2 96 rxn 60 000 MM201 RASSF1 96 rxn 60 000 MM201 SNRPN 96 rxn 60 000 MM201 TFAP2A 96 rxn 60 000 MM201 TMEFF2 96 rxn 60 000 MM201 TWIST 96 rxn 60 000 MM201 Custom 96 rxn 72 000 MM201 Custom 18 1 8 Cosrmo Bio News No 76 5 3 N A DI 3 N A X DNA DNA ICM DNA ICM In vo Complex of Methylase vivo DNA DNMT DNA DNA
113. R mir 1999 Nhel S 124 000 100Z2 SD 123 000100 AM 2 pEGP miR pEP miR EF 1 2009 9 600 GFP Puro GFP _ pEGP mir 52 Puro pEP e i iR MIR MMU MIR 0 Origi S miHNA pre miRNA
114. RSD AF5715 ESA SBS E1590 EXOC 3 PGI 14703 1 AP F FABP7 PGI 14836 1 AP FBXO36 ABG AT2019A FBXW11 PGI 13149 1 AP FFAR2 KAL KX411 FGFBP2 PGI 13254 1 FGFR3 BWT BS1125 Fibulin 4 BWT BS3119 FIGNL 1 BET A300 772A FMO5 PGI 13699 1 AP FMOD PGI 13281 1 AP FOLR1 RSD AF5646 FoxC1 2 BWT BS2300 FRMPD1 EVR EB09075 FSCN1 PGI 14384 1 AP FSCN3 PGI 13536 1 AP FTL EVR EB09092 FXYD1 PGI 13721 1 AP Fyn p59 SBS E11000 G GS3BP 2 BET A302 040A GAA PGI 14367 1 GABA RB BWT BS1134 GABBR1 ABG AT2138A GAD1 2 BWT BS1400 GAN PGI 14305 1 AP GAPDHS PGI 13937 1 GAPex5 BET A302 115A GBP5 PGI 13220 1 AP GCS SBS 16740 GFM1 PGI 14274 1 AP GFP RKL 600 145 215 GHRL ABG AT2199A GINS3 PGI 15651 1 AP GMFG ABG AP7871A Ga olf BWT BS3310 H HJURP PGI 15283 1 AP HNRPQ ABG AP7852B INP4B EPT Jan 12 K KCND 1 PGI 13768 1 AP Keratin 17 36 BWT BS1203 M MAEA PGI 15238 1 AP METTL3 PGI 15073 1 AP MFNG ABG AT2853A MGEA5 PGI 14711 1 AP MLXIPL PGI 13256 1 AP MRGPRC BBT PA1208 MTNR1B EVR EB09089 MYBPC1 ABG AT2944A MYO7A ABG AT2960A MYO7B PGI 14467 1 AP MYSM1 BMO 0660 0025 N NAPB PGI 14106 1 AP NCSTN PGI 14071 1 AP NKCC2 SCB 133823 NNAT PGI 15259 1 AP 150 150 pk 100 Lg 150 150 150 150 pk 0 1 mg 150 pk 150 2 000 test 150 100 Lg 100 Lg 100 Lg 100 100 Lug 150 pk 100 ug 0 1 150 pk 150 0 1 mg 150 50 g 150 pk 100 Lg 100 Lg 0 1 mg 150
115. X Y saDbioscienceS cosmobio CO jp PCR PCRH ABI 7000 Human Breast Cancer DNA Methylation PCR Array Signature Panel 12 MeAH 01 1X Y 1 MeAH 01 1A Y PCRH AG MAH 01 1A 12 2 1 4 4 Methly ProfilerrV PCR Cp DNA PCR CpDG 13 000
116. hN2 Human Neurons Discovery Kit AB2 M Human Neural Culture Medium Kit 2 Cosrmo Bio News No 76 NEW NutriStemTV ES MatrigelrW MEF hESC hESC H9 2 16 13 2 1 h ESC 50 h ESC hESC bFGF TGF8 5 C02 1 hESC XF HH1 hESC 10 20 Biological Industries Ltd BLG 05 100 1A 500 5 35 000 05 100 1B 100 mg 9 000 05 102 1A 500 27 000 05 102 1B 100 mg 7 000 hN2TM HN60000 hN2 Human Neuron cells 1 1 x108
117. http www cosmobio co jp TEL 03 5632 9610 FAX 03 5632 9619 TEL 03 5632 9620
118. it 96 test 95 000 Zap70 phospho Tyr492 ELISA Kit 6113 1 1 kit 96 test 95 000 PBK ELISA Kit 6112 1 1 kit 96 test 95 000 c Jun ELISA Kit 6111 1 1 kit 96 test 95 000 Histone H2B acety Lys5 ELISA Kit 6110 1 1 kit 96 test 95 000 Cosrmo Bio News No 76 3 http www cosmobio co jp campaign SA RNAi G 5920FF 009 9 14 12 28 microRNA PCR shRNA siRNA siRNA 825 OFF 50960FF 009 7 1 9 25 1 300 309 0FF 00 803 108808
119. lexes Methylation Ltd ICM1 50 assay 113 000 ICM1B 50 assay 113 000 ICM2 50 assay 113 000 ICM3A 50 assay 113 000 ICM3B 50 assay 113 000 3 Eiocnaire BioChain DNA DNA 72 DNA DNA 9996 DNA 500pg 2ug 3 ACS 500 STS 500 CVR 5tubes EQB 20mg
120. y Taq DNA Be i Arabidopsis columbia Arabidopsis columbia ie Chili pepper Capsicu 28 gt 15 WDNA Monstera Phire Hot Start DNA Polymerase TE Harris Uni Core amp Cutting Mat ldol 5 EN 2 Physcomitrella patens 2
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