Develosil HPLC 用充填カラム 取扱説明書
Contents
1. H 0 45um ce pH pH2. Develosil HPLC ILA AA 78 FECT DSW B Fr nA a RY F A D 20mm 2 T 489 0004 15 TEL 0561 48 1853 FAX 0561 48 1434 E mail info develosil net Develosil PACKED COLUMN INSTRUCTION MANUAL Introduction Thank you for purchasing our Develosil Packed Column We recommend you to read this manual carefully to ensure maximal e efficiency an
3. pH2 7 5 J pH Develosil ODS HG pH pH1 8 Develosil ODS UG EH pH pH2 10 Develosil RPAQUEOUS AR pH pH1 7 Develosil RPAQUEOUS AR C30 EH pH pH2 8 ODevelosil CN CN UG O 25MPa 1 50C 1 0
4. that we can t guarantee the lifetime of columns nor can we accept any claim when their performance has deteriorated due to noncompliance with the above operating instructions NOMURA CHEMICAL CO LTD 15 Hinode cho Seto Aichi 489 0004 Japan TEL 81 561 48 1853 FAX 81 561 48 1434 E mail info develosil net
5. Develosil HPLC Develosil HPLC Develosil HPLC 1 E
6. d long life of the column Confirmation of columns OVerify that there are no irregularities in the external appearance and packaging of the column OlInspect the column to assure you received the correct column size and packing Handling of columns Do not drop or hit the columns because severe shocks can cause cracks or channels in the packing which can result in decreased column efficiency Installation of Columns Before installing the column replace the solvent in the pump and the flow line with the mobile phase that will be used Connect the column according to the flow direction indicated on the label OTo reduce dead volume be sure that the injector and detector connectors have a small diameter and are as short as possible Mobile phase OlImpurities in mobile phase cause ghost peaks and lead to noise as a result of loss of light intensity when using UV detectors If possible always use high purity solvents Ocheck that the salt doesn t precipitate when mixed with organic solution before use OMobile phase containing insolubles should be filtered prior to use Degas all solvents prior to use to prevent malfunction of the pumps and to avoid detector noise caused by air bubbles Tf the column operated at temperature near the boiling point of the mobile phase detector noise may be generated from the mobile phase bubbles pH O Develosil HPLC columns are designed for use in the pH range of 2 7 5 To preven
7. t early deterioration don t exceed this range As for the following column a different pH range is set Develosil ODS HG pH1 8 Develosil ODS UG pH2 10 Develosil RPAQUEOUS AR pH1 7 Other C30 column series pH2 8 ODevelosil CN and CN UG have to use methanol solution because lifetime to do short with acetonitrile solution Sample Whenever possible samples should be dissolved in a solvent that has the same composition as the mobile phase OPlease use the temperature to 50 degrees Celsius OSamples containing insolubles should be filtered prior to injection Analysis OKeep pressure under 25MPa When use the preparative columns I D 20mm please confirm column pressure limit at test report OThe temperature please assume 50 degrees the upper limit OFrequent changing of the mobile phase composition will accelerate deterioration of the packing Storage of columns OIf mobile phase containing buffer have been used the column should be first rinsed thoroughly with organic solvent water and then purged with acetonitrile water or methanol water before storage OWhen dismounting the column be sure that the manometer reading is 0 OWhen storing columns first seal them with the plugs provided and then place them in a stable temperature location Develosil Columns are manufactured inspected packaged and shipped under strict standards of quality control Should you find any defect in performance please contact us However
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