取扱説明書 - 石原産業
Contents
1.2. 1 4 8C 777 HVJ E HVJ E Pb 3 0 5mL 10mL CFO01 VHVJ E HVJ Envelope 2 endotoxin free 20 2 HVJ E 1 100 B cell hybridoma HVJ E 1 10 3 HVJ E HVJ E HVJ E 2 80 3 1 4 HVJ E RNA HVJ E
3. 5 5 1 HVJ E HVJ E HVJ E 0 26mL endotoxin free 20 20 5 2 Suspension Plating B cell hybridoma Suspension Step 1 3 on ice 1
4. HVJ E Plating Step 1 6 on ice A B 6 well plate A 1 2x10 cells 25L HVJ E 2 5 u L Onice 5 lt HVJ E A gt 4C 2000rpm 5 1 1mL on ice 1 B Cell sheet HVJ E A 4 1000rpm 5 37 C 15 gt 1 5 2 mL 20 20 HVJ E
5. 37 C 15 gt 37C 109cells 50mL 96 well plate 100 ux L well HAT step 7 20 20 Step 2 myeloma 10 1 1 1 Step 5 HVJ E 0 5 2 TEL 06 6444 7182 FAX 06 6444 7183 E MAIL HVJ E iskweb co jp URL http www iskweb co jp hvj e Ver1 02 4
6. 0 5 10L 3 plate B cell hybridoma Step 4 6 on ice gt RPMI 1x 108cells Myeloma gt 1x 107 cells RPMI myeloma 10 1 50mL 4C 1000rpm 5 1 10 cells 1mL HVJ E 1mL 25L Onice 5 lt HVJ E gt 4C 1000rpm 5 gt
7. A B 2 x 10 cells 25 L 2mL HVJ E 2 54L Onice 5 HVJ E gt 37C 15 5 gt 1 5 2 mL 6 well plate 20 20 HVJ E 0 5 10L Step 3 4 2000rpm 5 Step 4
8. HVJ Envelope Cell Fusion Kit 72 7777 S gt HVJ E
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