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Cre-X-Mice Database User guide
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1. 11 Pieure 16 Main User Titer aCe i esionee teo iota eksanbn anesen mass deas o uva a oua o gap ER NAE 12 Figure 17 Porm tor Adding QUeFIOS duse iiie eb oiii ava seksini e cra oi oaa aoc uua va ies inilen saad i bkm 12 Figurel8 serMmterfacefor ueryEditon 16 Figure 19 Example of Updated Queries iiit etie temi Die lah eksi uku dak i komik ilah d n k an i yuku S kan 14 ii Introduction Cre X Mice went through a large update in the month of December there were many modifications and new features introduced Searching the database 1s now possible by the use of keywords as well as the advanced mode When a new transgenic line is submitted an email is sent to the author for automatic verification Cre X Mice now supports user registration There are many benefits to being a registered user you can receive notifications about new entries added to the database view and edit your own entries and obtain user feedback by e mail on specific entries It 1s strongly recommended that you register 1f you use the database frequently or if you are the author of any entries in the database User feedback is now available for the transgenic lines as well as a general discussion board on the main page This guide will describe all these new features in detail and should prove useful to new users as well as frequent visitors Searching the Database To access the search page click Search
2. To begin the process of submitting a new transgenic line click Submit Transgenic Line on the left side menu The browser will be redirected to step one of the submission process Step 1 Notice to select multiple options hold ctrl and use left mouse click the selected options will be highlighted blue 1 Transgene Type There are three possible options for Transgene Type please select one 2 Promoter or Locus Start entering the promoter the database will look up existing promoters and provide you with suggestions If there are any synonyms for the promoter please enter them below separated by a comma 3 Promoter Species Select the species from the dropdown list For species that are not listed select Other and specify the unlisted specie below 4 Transgene Insertion Enter the transgene insertion here 7 8 9 Inducibility Select indcibility from the list you may select multiple options For options not in the list select Other and specify the inducibility below Genetic Background Select genetic background from the list you may select multiple options For options not in the list select Other and specify the genetic background below Is this Cre Transgenic Line Published Published transgenic lines require PubMed ID and author contact information for verification purposes If the line is published click yes and fill out the required information below MGI ID Enter the Mouse Genome Informatics ID
3. The first name or last name field is blank please fill in both fields You must enter an email address 14 The Email field 1s blank an email address must be provided for registration confirmation and contact information You have entered an invalid email address The Email address provided 1s not a valid email address make sure you have entered the email address correctly An email must be in the following format username domain top level domain Example of a valid email address cre mice nagy com in which cre mice is the username nagy 1s the domain and com is the top level domain Email and email confirmation do not match The Email and Confirm Email fields are not the same make sure you have entered the email address correctly in both fields The email address is already in use This email address already belongs to a registered user or an author of an entry If you are an Author of an entry you are already in the database Log in using your email and author as the password field and click on the Resend activation email link The email address entered belongs to an author of a transgenic line This means that this email address was given as contact information of the author Therefore your information 1s already in the database please go to the Log In Register page enter your email and use author as password You will receive a warning message This user account has not been activated an activation email has been p
4. click the Add Queries button right below the table A form shown in the following figure would be prompted Add Your Queries Promoter Locus Anatomical Area Po Cell Type Choose from menu sw Other Cell Type Po Genetic Background Choose frommenu l Other Genetic Background Po Author Po Other Po SE Back Figure 17 Form for Adding Queries The user could submit up to eight queries at a time After the form is filled click Save to get back to the main user interface as illustrated in Figure 16 A row of new entry would be shown at the top of the table because the table follows the descending order for dates The user could also click Back to go back to the main user interface without adding any queries 12 Editing Queries The user could first click the Edit Queries right below the table and beside the Add Queries button in the main user interface Next a user interface for query edition is shown below My Requested Queries Please click on column headings to sort Each Update column shows query status respectively since you last add edit the query Other Cell Updated Link Promoter Anatomical Update ms pdt Cell Genetic Other Gen Query nk cus Update Area Update Type Background Update Back Update Author Update Other Update Date J J 2011 04 04 Camk a No No Neuron Wo No No No No No 03 30 28 Edit Back Figu
5. Database on the menu bar Quick Search Mode With the last update it is now possible to search the database based on key words Enter key words in the Quick Search field and click the search button Quick Search Figure 1 Quick Search Example Advanced Search The advanced search window provided users with detailed categories to filter the results Advanced Search Anatomical Area Excision Image Descriptions Cell Type Al lt tell Excision Area Excision Stage is on or after e At Stages w B ui cel B A elis Omnpisete Promoter or Locus D almost Al Cells s Patchy Mosaic Transgene Type Al l Majority Of Cells 3 Fine Mosaic go Minority C ell Scattere Inducible Only yes f d rew eis apecific Location Genetic 129 A No Ce E Not Dete Background BDF1 Not E 4 C57BL 6 i CBA T9 Y ta e CD1 Y j PubMed ID MGI ID Author Sort By Promoter or Locus Search Reset Figure 2 Advanced Search Example Viewing Search Results Once the search 1s complete the search results will be displayed at the bottom of the page in collapsed form You may click on the blue folder to expand or collapse all results Found 3 Results Click v to see details of a specific entry G to viewa particular entry Shov Hide all details Ld Verified by Autho e d p i View Add Promoter Author Unverified by Author D odiad bar Butlers
6. here this field is optional and may be left blank Holding Sites Optional field and may be left blank 10 Stock Number This field relates to the holding sites Enter the stock number or a url to the page Example of a filled out step one form is provided as a reference Submit Cre Transgenic Line Step 1 of 3 Please complete the following information Transgene Type Knock in e Genetrap Random Insertion Genetic Background pecify unlisted Genetic Backgrounds below eperate multiple entries wth a comma Not Listed Genetic Background Is this Cre Transgenic Line Published Promoter or Locus No 9 ves PubMed ID 2345 Author s name 3 Author s Email Address Promoter Species author email com Baboon swe MGI ID Optional Transgene Insertion Pe FHCRC Inducibility Non Inducible Other RU486 Holding Site Stock or URL Optional Jamoxiten http jaxmice jax org strain HEHE html Figure 12 Submission Step 1 Example Once all the required information has been entered click continue at the bottom of the page The database will check that all the fields are correct and proceed to step two 8 Step 2 Step Two is where specificities are added to the entry At least one specificity must be added before proceeding to step three and submitting the transgenic line Once all needed specificities are added click Next Step For every specificity the f
7. Comments Comment 4 v A tau Andras Nagy v Aaabb add 8 v Salt Michael Tauc a Aaabb add 1 amp a CCSP Michael Tauc a Aaabb add Figure 3 Search Result Collapsed View Let s look at the very first result 4 is the number of specificities this entry contains Green folder expands and collapses the specificities A Redirects to viewing this transgenic line and all the details Then we see the Promoter and the Author of the entry The author verification section exists because not all the entries in this database are submitted by the creator of the transgenic line Author Cre X Mice does try to contact the Authors and have them verify all the information View Comments section allows to open a new page showing all the comments if there are nouser comments for a specific like the button 1s grayed out Anyone may add a comment to the entry by following the add link under 4dd Comment Click the green folder to expand the first result We can now see all the specificities the entry contains By clicking on the headers Stage Cell Type and Anatomical Area the specificities are sorted to make viewing easier for the users 4 X A tau Andras Nagy v Aaabb add Stage Image Cell Type Anatomical Area 9 0 dpc visceral endoderm yolk sac lt extraembryonic component 12 0dpc Neuron forebrain lt brain lt central nervous system lt nervous system lt organ system lt embryo 14 5dpc m Neuron brain lt venous sys
8. Cre X Mice Database User guide Table of Contents ToO uu c RG RP e PE e ee ii a 0 OPPE TE EO EO e Do Doo DEOR 1 MC RC Pala BEIC T 1 Cuick ede OO OE K VR pe Su UM i eid d s 1 Advan CO S e qM HE e 1 a alp a e aea RE l EE e 2 Rohs To PLU EOS T 3 bios eto t PH 4 Editino Your ACOG e e reer reer 4 PONS NNT emm o o o mm 5 ay do EA ETE t ome 5 Discussion Board and User Comments io iiic Praes saute ax si layla S yle ula tmu Bala yasa Sy l 6 Ah O A A 6 Commenting ona Transgenic Line eker mama a ore tt ee la an e aa ank 6 Submitting a New Transgenic 7106 eoe de eset te epit asal enimisdisnelan neliiassleninidisene RAEE aa E iaa 7 SUA Tr m see ce 7 Sie D 9 OLD T T 13 Update KE Ei Esiin RS a trer cere 11 a O E KM RE 11 MEME E E EE E EE E e T 12 Adde e TU 12 FEIN OS TNS E EE 13 Benne tay a oc pee eee ener er TOO OO TQ ee 13 ICG itis P Pe ey eee 14 ONUS RR oom al yi s a a ey ine i 14 e er gl e 17 Editing Account Error Messages nein tuin trad eun tura a ana d tun aaa i caduto deua Ua quad dun usus dil ouluda a dsam 16 Table of Figure Figure L Ouick 5 Cane Ham
9. TA1 Vladimir Joukov P 1 Aaabb add 3 V Q ACTA1 Vladimir Joukov a jAeabb add 3 Q ACTAl Vladimir Joukov Aaabb add Figure 6 User Account Example Editing Your Account Once logged in click on the Edit Account button there you will be able to edit all your information When you click on Save if an error message appears please see Editing Account Error Messages for more information An example of filled out Edit Account form to change the password 1s provided as guidance Cre X Mice A Database of Cre Transgenic Lines Main Page Search Database Edit Basic Information Submit Transgenic Line First Name My Account Log Out Last Name Nagy Lab Homepage Edit Email or Password Contact Us Email bw Verify Email MOUNT SINAI ag HOSPITAL Current Password New Password Verify New Password Figure 7 Edit Account form with password change example Editing Your Entry 1 Registered users are allowed to make changes to the transgenic lines they have submitted To edit your entry you must first log in See Logging In for details After logging in find the entry you want to modify and click on the view icon Scroll to the very bottom of the page and click the Et Edit button 2 If you are verifying an entry because you received an automated email from Cre X Mice you may also edit the information After following the link scroll down and click the Edit Edit button This will
10. cular query The following is an illustration Updated Promoter _ Anatomical Cell Other Cell _ Genetic _ Other Gen mn Link dies Update am Update Twe Update Tini Update Bars Update Back Update Author Update Other Update Date 2011 04 01 fru P V P T n Camk2a Yes No Oo Neuron Yes No No No No No 5 13 35 Promoter E ri Locus l 2011 04 01 F Camk2a 2011 04 01 No No No No No No No NO inan 15 18 35 E Cell T d Neuron 2011 04 01 Na Na Na Na Na No No No vade 15 19 35 ae te Figure 19 Example of Updated Queries In this example since an entry that contains information regarding the promoter locus Camk2a and the cell type Neuron has been updated the query status of the corresponding requested queries are changed to Yes At the left side of the table the updated queries are shown with the embedded links for the access of the updated entry which specify the categories of the updated queries and the date of the entry update In addition the user would receive a notification email regarding the updated entry with the embedded link Appendix A Registration Error Messages When submitting your registration form you may receive an error message this section explains the errors and steps to correct them Notice password and password confirmation must be reentered when resubmitting your form after correcting the errors List of possible errors You must enter first and last name
11. main page or contact the database administrator directly at jouk vlad gmail com Editing Account Error Messages List of possible errors Email does not match Verify Email Make sure both Email and Verify Email are the same and that you have entered the email address correctly New Email is not a valid email address The email address is invalid Cre X Mice database requires all users to have a working email address for contact and security purposes Make sure you have entered the email address correctly Example of a valid address cre mice 2 nagy com Incorrect Password You have made a mistake while entering your current password Current password is required when changing passwords for security reasons New Password and Verify New Password do not match The new password and verification of the new password are not the same Please choose a password that will be easy for you to remember and verify you have entered it correctly Failed to update account ID This 1s a server error Please try again in a while If this error continues to repeat please contact the system administrator through front page discussion board 16
12. mission by clicking Submit Entry Once all the information 1s verified press Submit Entry Every new entry must be approved by the database administrator before going into the public database and should be verified by the author A verification email is sent to the author if publication details are given see Verifying Your Entry for details 10 Update Mailing List This recently added feature 1s used to notify users about the new queries updated into the database It 1s recommended for the user to subscribe to this update mailing list if certain queries requested by the user do not return any results in the search or if the user wants to be notified about the most recently updated entries Once an entry which contains particular data that match the user s requested queries is updated the user would receive an email notification regarding the information of such entry Registration The form for update mailing list registration 1s right below the Advanced Search form There is a brief instruction at the top of the form that explains the purpose of update mailing list and its related features The required fields include user s first and last name up to eight queries user s email address and word verification The user can also unsubscribe to the update mailing list if he she has previously subscribed in which the email address 1s the only required field in such case Update Mailing List Cannot find the results you are lo
13. oking for Want the most recent results You are then welcomed to subscribe to this update mailing list in which you would receive a notificaiton email once the entry which has the data that match your queries is updated You can submit up to eight queries when you first subscribe to this update mailing list If you are a member please use your registered email address for subscription so you can edit your queries in My Account Log Out section after your login Otherwise you are still welcomed to subscribe but your queries could not be edited unless you register as a member using the same email address You could also unsubscribe to this update mailing list through here if you have previously subscribed Please enter your first and last name up to eight queries email address and word verification First Name Last Name Promoter Locus Anatomical Area Cell Type Choose from menu z Other Cell Type Genetic Background Choose from menu iel Other Genetic Background Author Other Your E mail Address Subscribe Unsubscribe Word Verification Please help us prevent false entries by typing the words you see to the right into the field provided Submit Form Reset Figure 15 Update Mailing List Registration Form Once the registration form 1s submitted successfully a confirmation email would be sent to
14. ollowing steps must be taken 1 Select the stage the characterization was performed at Once a stage is selected mouse anatomy loads showing all the anatomical areas possible in that stage of development Specificity Characterization Was Performed At 4 5 dpc x 2 The second step is to select the anatomical area It is possible to search for anatomical area or to expand the tree manually Green folder gt and green checkmark represent expandable sections and end points of anatomy tree respectively To browse through the tree manually click on the green folder to expand an area and on the name to select that area Another option is to search through the tree enter a keyword in the search field and press the search button Found anatomical areas will be highlighted in and the tree will be automatically expanded Selected anatomical area will be highlighte nd the full path will be shown Mouse Anatomy mouse embryo V unti primitive endocerm embryo Search Selected Anatomical Area epiblast embryo Figure 13 Step Two Stage Selection Above and Anatomical Area Selection Below In this example keyword embryo was searched for and epiblast was selected as the anatomical area 3 Select the excision property from the list You may select multiple options by holding ctrl and using left mouse click to specify a range 4 To select the cell type start entering text in the field the database will perform a
15. p IO a ss alaz ala asel ada Madak lalesi aaa 0200000429000 900 0209048 I Feo 2 Ance d oed c EXAMP EY EE RE EREL ME YO E E RAE EPS I Figure 3 search oV RAO T p 2 Fiseure 4 Expanded Search Result ieiesses esses ves ine nirre Sons had usua enis uc eb dud ea aas had vno doe quao Da ooh Tode a geo inden 2 Ireure 5 Registration Form Examples ems sl due mus pesese i pudenda ilede ll lana Bain lemek lem anes 3 Figure o User Account Edi D E cs o larak ille sete unoque dai Papae La dea nes ilik sadabile esddeiiaisdlmilaslasili si adakila i sda dacdazi 4 Figure 7 Edit Account form with password change example sss 4 Fig re 9 very Entry Page CODUORS cocoosseooooeseo200205902909220049022 020203230020023850 4 042200 020 2000038 00 2 0 9 9 4 9129800 000 99 9048 3 Ere gt V ri Ete b en ides yi eke am A alan alam akan sekte acan 3 Fig re TO Discussion Example sss sarsmaya asa eaim sanaya EE EEE EEEE EERE EEEE E 6 Figure Li Comment Form Example uuieciosio emma ier E S O S 7 Erte 12 Submission Step TB K sale a lam ala ERRE alamam lane ERAEN EEE ER earner rr 8 Figure 13 Step Two Stage Selection Above and Anatomical Area Selection Below 9 Figure 14 Step Two Example for Step 3 5 above and Finished Specificity with Delete Button below P 10 Figurel5U pdateMailinglistRegistraatlonFom
16. r Comments Discussions A discussion board was added to the main page of Cre X Mice database As soon as main page loads the latest twenty are shown The discussion board is organized in a tree format so once a post is started replies may be directed to the post or to any reply Any user or visitor may start a new post and reply to the already existing posts The following figure shows a discussion Discussions Latest 20 New Post All Posts Author Guide Jan 16th 2010 16 07 Vladimir Joukov guide message Email jouk vlad gmail com Organization Reply Expand replies Figure 10 Discussion Example To create a new discussion click on the link New Post A new discussion must contain a title and a message User or guest information must also be provided To reply to an existing discussion click Reply A title for a reply 1s optional it may be left blank User or guest information must be provided To view all discussions not just the latest twenty click All Posts Once redirected to the page containing all of the Cre X Mice discussions it is also possible to search for key words To view all the replies click Expand replies Notice some replies may be then further expanded Expanded discussions may be collapsed by clicking Collapse replies Commenting on a Transgenic Line When viewing an entry in the database users and guests who have knowledge of that transgenic line are encouraged to leave comment
17. re 18 User Interface for Ouery Edition The user could select a specific entry reguired for edition then click Edir button and the form which is similar to Figure 19 would be prompted The existing gueries would be shown in corresponding fields and the user could edit the existing queries and or adding more queries by filling in the form Next click Save to get back to the user interface for query edition If the query edition is complete the user could click Back right below the table in Figure 18 to get back to the main user interface illustrated in Figure 16 Deleting Queries There are checkboxes next to each specific row of queries The user could first select the as many queries as needed by checking off the checkboxes Afterward click the Delete button at the top of the table shown in Figure 16 to delete the selected queries It is recommended that the user deletes repeated queries or any queries that are no long requested in order to prevent unnecessary notification emails We would greatly appreciate your help in making the update mailing list as user friendly as possible by minimizing the number of unnecessary queries The user could unsubscribe from the update mailing list by deleting all of the requested queries 13 Keeping Track of Queries Each update column next to the every requested query shows the query status of whether or not it has been updated since the user last adds or edits that parti
18. redirect you to Submit Transgenic Line page with the information already filled out Follow the steps as if creating a new entry See Submitting a New Transgenic Line for details Once you submit the entry you will need to verify it a verification email 1s automatically sent to the email address provided The modified entry also needs to be confirmed by an administrator once that is done it will permanently replace the old one Verifying Your Entry 1 Registered users are allowed to verify their own unverified entries Log into your account and click on the view icon of the entry you want to verify Under Publication Details find This 1s your entry please take a moment verify it verify click on verify Publication Details PubMed ID 1234 Author Name Vladimir Joukov Author Email jouk vlad qmail com amp This entry is in the process of being verified by the corresponding author This is your entry please take a moment verify tyerity Comments Some Comment 2 Figure 8 Verify Entry Link 2 If you received an email from Cre X Mice requesting you to verify a new entry in the database please click on the link provided in the email This will direct you to a page showing the new entry Please make sure all the information is correct You may choose to verify edit or decline the transgenic line at the bottom of the page see Editing Your Entry for details Figure 9 Verify Entry Page Options Discussion Board and Use
19. reviously sent to email Resend activation email Resend Click on Resend then check your email for account activation from Cre X Mice Once you have activated your account you may log in using your email and author as password please proceed to Edit Account and change your password from author If you need more information on changing your password see Managing My Account section You must enter a password The password field 1n empty a password must be entered Your password must be at least 6 characters in length The password you have entered is too short password of minimum 6 characters 1s required for security purposes Password and password confirmation do not match The Password and the Confirm Password fields do not match The Confirm Password field is designed to make sure the user enters a password he or she will remember Please make sure you have entered the same password in both fields Your word verification failed Please try again 15 Word verification is designed to make sure that spam software does not register as a user on the database and post unwanted ads and comments You have not correctly entered the two displayed words please try again You may request new words by clicking on the icon Server error could not create user This error appears if there is a problem with the server Please try again in a while if the registration still does not work please reply to the bugs discussion on the
20. s To comment on the transgenic line scroll to the bottom of the page to Post a Comment form A valid comment must contain a Subject a message and the information of the poster Notice Cre X Mice will only display your email address and organization if you check the Show My Information checkbox Otherwise only the first and last name will be shown 1 Asa user if you are logged in while posting a comment Cre X Mice will automatically use your information for the comment simply enter the subject message body and check 6 Show My Information if you wish others to see your email and organization Once the form is complete click Submit Comment 2 If you are a user but you are not logged in please expand the User folder and enter your email and password Fill out the rest of the form and click Submit Comment 3 Visitors without a registered account are required to provide their first and last names and an email address organization 1s optional If you are a visitor please expand the Guest folder and fill out the form Below is a filled out comment form to use as a guide Post A Comment Subject Guide Message Body For creating a comment for a transgenic line Your Information Guest User First Name Test Last Name Test Email valid email com Organization Optional Submit Show My Information V Submit Comment Figure 11 Comment Form Example Submitting a New Transgenic Line
21. s redirected to Log In page and the following message is displayed Registration successful An activation email has been sent to email The account now needs to be activated Check your inbox for an account activation email from Cre X Mice click on the link in the email to activate your account You will be redirected to the Log In page with the message Your account has successfully been activated Registration is complete use you email and password to log in Your Account Being a registered user has its privileges you can modify your own entries and request email notifications for newly added transgenic lines Soon a new feature will be implemented so that registered users can monitor specific entries the user will be notified about any comments and any changes Below is an example of what the user will see after logging in Cre X Mice A Database of Cre Transgenic Lines Main Page Search Database Account Information Submit Transgenic Line First Name Visdiriz My Account Log Out Last Name Joukov Credits Email jouk viad gmail com Nagy Lab Homepage Organization Contact Us EditAccount Log Out My Entries when viewing your own entries you may modify and verify them aw MOUNT SINAI e T HOSPITAL me Found 3 Results Click to see details of a specific entry Q to view a particular entry Show Hide all details Verified by Author Promoter Author E Unverified by Author Eo E eco RR Modified by Author 3 qo Q AC
22. suggestive search to help If you do not see required cell type in suggestive search enter the cell type manually it will be added to the database 5 Select the cell amount cre expression occurred in To select a range between two or more options hold ctrl and use left mouse click Please define the excision property Cre excision expression occurred in Choose from list Few Cells Majority Of Cells 50 75 Majority Of Cells 75 Minority Of Cells 25 50 Minority Of Cells lt 25 No Cell Not Determined Cell Type McnxCel 5 6 Click add specificity the page will reload and added specificity will be displayed at the top If there is a mistake in the specificity it 1s possible to delete it by clicking the recycling bin icon Steps one through five must be repeated for each specificity to be added Submit Cre Transgenic Line Step 2 of 3 Del Stage Image Cell Type Anatomical Area 4 5 dpc Hel ie Acinar Call epiblast embryo NextStep Figure 14 Step Two Example for Step 3 5 above and Finished Specificity with Delete Button below Step 3 Step three provides an overview of the new transgenic line to verify all of the information The submitter may enter an author comment for the line select to be notified when the transgenic line is accepted into the public database return to any of the two previous steps to edit the submission or finish the sub
23. tem lt cardiovascular system lt organ system lt embryo 8 0 dpc Other ALL ANATOMICAL AREAS Figure 4 Expanded Search Result Registration The first step 1s creating a new account from the main page please navigate to Log In Register located on the menu bar to the left Once the Log In screen loads click on the Register button to proceed to the registration form Below is an example of a filled out registration form Please notice that Organization field 1s optional Fill out all the fields and click Register to submit the request If you are not redirected to Log In screen and receive an error message please see Registration Error Messages section for instructions Cre X Mice A Database of Cre Transgenic Lines Main Page Register Search Database Please complete the following information All fields are required Submit Transgenic Line An email will be sent to the provided address to verify your identity Log In Register tr T RE First Name Credits Nagy Lab Homepage Last Name Contact Us Organization 1 Email vlad 5 5 5 hotmail com bw MOUNT SINA LA Aa Confirm Email vlad_5_5 5 hotmail com Password Confirm Password Word Verification Please help us prevent false entries by typing the words you see to the right into the field provided Type the two words yar pal ise ciopped ges t o e Register Figure 5 Registration Form Example Once the registration form is accepted the browser i
24. the user s email inbox using the email address specified The content of email displays the corresponding queries that have been requested 11 User Interface The user is welcomed to add edit and or delete any requested queries through the update mailing list user interface If the user is not a registered member of Cre X Mice he she must use the exact email address used for update mailing list subscription to register as one under Log In Register in order to edit the requested queries Otherwise registered member could first log in to access the Account Information page in which a section of My Requested Queries is displayed at the bottom half of the page My Requested Queries Please click on column headings to sort Each Update column shows query status respectively since you last add edit the query Please eliminate repeated queries and or queries that are na longer requested in order to prevent unnecessary notification emails You could unsubscribe from the update mailing list by deleting all af your queries Delete Updated Promoter Anatomical Cell Other Cell Genetic Other Gen Query Link in Update Area Update Te Update Type Update Baid Update Back Update Author Update Other Update Date 2011 04 01 Es la la Mi n dn m ri T CamkzZa Na No Neuron Ma Na No No No No 15 17 41 Add Queries Edit Queries Figure 16 Main User Interface Adding Queries The user could add as many queries as needed First
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