User Manual Lx 500
Contents
1. 3 Glare visible in field of View The condenser is too low Raise condenser light The condenser iris diaphragm ring is Adjust the aperture according to the closed objective magnification Pg 18 4 Observation image is hazy The objective is not engaged in the Engage the objective into position or unclear light path until it clicks Pg 18 The objective eyepiece condenser Clean glass parts thoroughly with lens and or specimen glass is dirty tissue and cleaning cloth Immersion oil is not used with an Use immersion oil as immersion objective suggested Pg 19 Bubbles are present in immersion oil Remove the bubbles by agitation Pg 19 The specified immersion oilis notused Use the immersion oil supplied by LABOMED 5 Part of image is defocused The objective is not properly engaged Engage the objective into position in the light path until the nose turret clicks The specimen is not set properly on Set the specimen correctly on the the stage stage and secure using the specimen holder Pg 15 6 Coarse focus adjustment cannot The condenser is too low Raise the condenser lower the stage low enough 7 Fields of view through both The interpupillary distance is not Adjust IPD to the appropriate setting eyepieces is inconsistent adjusted properly Pg 17 Dioptric compensation for the two eyes Adjust diopter settings Pg 17 is not set The left and right eyepieces are of Ensure that both eyepieces are of different mag2. tT we Fig 3 4 Do not disassemble any part of the microscope as this could result in malfunction or mitigated performance When not using the microscope ensure that the frame is fully cooled before storing the unit in a dry locker or covering with a dust cover provided To clean the condenser fully loosen the securing knobs and remove the condenser by lowering condenser bracket then wipe the front lens of the condenser with optical cleaning solution mixture suggested above and lens tissue The condenser can be re attached by replacing the condenser in its seat tightening securing screws and raising condenser bracket to desired position Lx 500 Care amp Maintenance Your microscope has been engineered for a long and safe operational life with the least amount of maintenance required In general routine maintenance is limited to keeping the microscopes working parts lubricated and optics clean Always cover the microscope with the provided dust cover when not in use Optical Cleaning 1 The objectives have been adjusted for a tight fit to prevent any damage during transportation To remove an objective rotate it counterclockwise while gripping it with a rubber sheet etc to avoid any slippage 2 To clean the lens surfaces remove dust using a soft brush or compressed air cans available at your local electronics store For removing finger marks or grease soft cleaning
3. Iris o i li l i i E i a Flip top condenser Polarizer Mount Analyzer Mount Blue filter Green filter Halogen bulb i 1 J van Installation and Operation of Optional Accessories iVu Camera Module System 1 Mount the Video adapter 1 2 part 3143300 912 on Trinocular observation head 2 Mount iVu Camera Module System on video adapter Fig 4 Lx 500 Optional Eyepieces 10X eyepieces are provided To replace 1 Pull out the 10x eyepieces out from the observation heads ocular tube 2 Insert desired eyepieces in empty ocular tube Fig 5 Phase Turret 1 Remove the condenser from its position by loosening the two thumb screws 1 on both sides of the condenser as shown in figure 6 2 Place the phase annulus turret in position from where the condenser has been removed IW gt 3 The long push in to engage thumbscrews 2 on both sides of the e phase turret are provided for proper alignment of the phase ring By disengaging these screws the phase turret can be adjusted at any desired phase annulus 10x 20x 40x amp 100x by turning the thumbwheel to the desired position There is also an 0 setting on the Ca thumbwheel for bright field application Note Refer to the user manual provided with the Phase Contrast Fig 6 kit for centering and alignment kw C INITIAL SETUP Objectives are factory set
4. cloth or lens tissue lightly moistened with cleaning solution 85 petroleum ether and 15 isopropanol should be used For cleaning the objective optics use Methanol Observe sufficient caution in handling Methanol Place the objectives and or eyepieces on a dust free surface e g aluminum foil All other optical components to be cleaned should be as accessible as possible 3 Blow all loose dust particles away with compressed air or mini dust blower Remove all water soluble dirt with distilled water If this is unsuccessful repeat using a solution of diluted hand soap liquid Remove any remaining residue with a dry cotton swab 5 To remove oil use a solution of diluted hand soap liquid initially If this does not produce a satisfactory result repeat the cleaning using a solvent Optical Cleaning Solution 85 petroleum ether and 15 isopropanol 6 Grease must always be removed using a solvent 7 Cleaning is achieved by using a spiral motion from the center to the rim Never wipe using zig zag movements as this will only spread the dirt With larger optical surfaces e g tube lenses the spiral motion starts initially at the rim before moving to the middle and is then followed by a center to rim cleaning motion Normally several spiral wipes are recommended We recommend pure volatile petroleum ether or Optical Cleaning Solution as explained in point 3 above zig zag motion X spiral motion v Wipe using a spiral movement Do not u
5. the slide glass and the front lens of condenser If oil is not placed there the observed image may appear dark 5 After use remove oil from the objective front lens by wiping with lens tissue slightly moistened with an ether 70 alcohol 30 mixture A caution If immersion oil makes contact with your eyes rinse eyes out thoroughly with fresh water If immersion oil makes contact with skin wash affected areas with soap and water If prolonged discomfort is experienced consult your physician immediately j 18 TROUBLESHOOTING GUIDE Under certain conditions performance of the unit may be adversely affected by factors other than defects If problems occur please review the following list and take corrective action as needed If problem persists please contact LABOMED or your local LABOMED dealer Observation Cause Remedy 1 Uneven brightness in observation The objective is not engaged in the Engage the objective into position field light path until the nose turret clicks The condenser is too low Raise up to achieve more light Pg 18 The objective eyepiece condenser Clean them thoroughly as previously and or window lens are dirty prescribed in Optical Cleaning pg 4 2 Dustor stains are visible in The eyepiece condenser window lens Clean glass parts thoroughly with lens observation field and or specimen glass is dirty tissue and cleaning solution prescribed in Optical Cleaning Pg 4
6. Adjust the interpupillary distance Adjust the dioptric setting Adjust the aperture iris diaphragm 7 Engage the objective to be used in the light path and bring the specimen in focus Engage the required filters Adjust the brightness Observe Specimen Lx 500 Fig 13 Placing specimen on the stage f Flip the main switch to I ON as shown in Figure 12 Rotating the light intensity adjustment knob Fig 13 in the direction of the arrow increases brightness and rotating knob in the opposite direction decreases brightness The intensity bar next to the knob indicates the direction of intensity level Rotate the coarse adjustment knob 2 in anticlockwise direction to fully lower the stage Open the bow shaped lever 3 outward by pulling on lever handle 1 place the specimen by sliding the specimen glass plate s on the stage from the front toward the slide seat atthe rear After positioning your specimen slides 2 max return the bow shaped lever 3 gently by slowly releasing control knob 1 Rotating the upper co axial knob controlling the Y axis movement 4 moves the specimen in the vertical direction Rotating the lower knob 5 moves the specimen in the X axis or horizontal direction Z Do not adjust the speci
7. LABOMED Lx 509 To ensure proper use of this instrument as well as to avoid injury while operating instrument understanding this manual completely before use is highly recommended CONTENTS INTRODUCTION The Lx 500 is a research microscope reflecting a modern design as well as the latest in optical and mechanical advancements Designed for professionals as well as clinicians this microscope offers many features and functions for a diverse set of applications Here are a few points highlighting the benefits of the Lx500 Extra clarity and contrast is provided through a 360 rotatable Binocular body inclined at 30 with IPD adjustments The pressure die cast stand consists of ball bearing friction based focusing mechanism to avoid any loss in motion The sturdy new stylish design provides a high degree of comfort as well as stability The high powered objectives are spring loaded to prevent accidental damage to specimen slides The reverse angle quadruple nosepiece has a comfortable ribbed grip for easy rotation that also safeguards the turret system against any damage All positions are par centered and par focalised ensuring the highest level of accuracy The ball bearing mechanical stage allows smooth travel over a 76 x 50mm area with spring loaded stage clamps for retaining the specimen at the exact position desired A 0 1mm vernier scale provides accurate location of specimen are
8. Objectives are par centered and par focalised during assembly All objectives have been secured for a tight fit to prevent them from coming loose during transit To remove an objective rotate it counterclockwise while holding it with a rubber grip to avoid any slippage Install the observation head using the following procedure 1 Using allen wrench 3mm provided loosen the Head Locking Screw 1 and remove the dust cover cap provided in dovetail cavity as well as on observation head dovetail 2 Mount the Observation Head by engaging the dovetail provided at the bottom of the head into the dovetail cavity provided in the microscopes arm 3 Tighten the Head Locking Screw 1 after positioning the Observation Head as desired See figure 8 Eyepieces Insert the eyepieces into the ocular tube of Observation Head using following procedure 1 Remove the protective caps from the observation tube 2 Insert 10x eyepieces into the spring loaded and to prevent undesired rotation of eye pieces See figure 9 11 Lx 500 ASSEMBLY Each standard set can be assembled by simply attaching the bulb filter and power cord Installing or Replacing the Lamp Bulb Before attaching the lamp bulb remove the parts that may drop such as the filter and specimen from the microscope frame and place the microscope onits back so that the bottom plate is exposed 1 Pull out the lamp house cove
9. a High power illumination is delivered through our well crafted Universal Power Supply operatable within range of 100V 240V AC input This ensures continuous operation even under fluctuating voltages Our Halogen bulb 6V 30W has an average life span of up to 500 hours The Lx 500 comes equipped with a removable flip top N A 1 25 Abbe condenser with aspheric lens for brighter illumination levels and an iris diaphragm for better resolution and contrast control Lx 500 SAFETY INFORMATION 1 After the microscope has been used for observation of a specimen containing bacteria clean all parts coming in contact with the specimen to prevent infection E Be sure to remove the specimen before moving this product E Incase the specimen is damaged by erroneous operation it is important to clean all surfaces that may have come in contact with the specimen Applicable bulb 6V30W Halogen bulb P N EL 455 3 Install microscope on a sturdy level table or bench and avoid any restriction of air vents in the base of the unit Do not place microscope on a flexible surface as this could result in blocking the air vents and cause overheating 4 Always use the power cord provided by LABOMED If the proper power cord is not used product safety performance cannot be warranted 5 When installing the microscope route the power cord away from the microscope frame Should the power cord come in contact with the m
10. ent with delicate glass components Please handle with care 2 Do not use the microscope where it is subjected to direct sunlight high temperature humidity dust and vibrations For the operating conditions see chapter 14 SPECIFICATIONS 3 The microscope is ventilated by natural convection Be sure to leave enough space 10 cm or more around body when installing the unit 4 Arm handle is provided for carrying the microscope A To prevent damage do not hold the microscope by the stage or observation tube Be sure to remove the specimen from the stage clip while transporting unit to avoid damage to the specimen slide 1 Clean all glass components by wiping gently with cleaning cloth provided To remove fingerprints or oil smudges wipe with cleaning cloth slightly moistened with a mixture of petroleum 85 and isopropanol 15 Since solvents such as petroleum and isopropanol are highly flammable they must be handled carefully Be sure to keep these chemicals away from open flames or potential sources of electrical sparks for example electrical equipment that is being switched ON or OFF Also remember to always use these chemicals only in a well and ventilated room 2 Do not attempt to use organic solvents to clean the microscope components other than the glass components To clean non glass components use a lint free soft cloth slightly moistened with a diluted neutral detergent 3
11. ge is observed rotate the fine adjustment knob 2 for fine detail focusing in anticlockwise direction Fig 17 Working Distance WD The WD refers to the distance between each objective and the specimen Objective Magnification 4X 10X 40X 100X WD mm 30 5 482 055 0 11 8 Lx 500 Adjusting the Interpupillary Distance IPD The inter pupillary distance adjustment consists of moving the two eyepieces to align with both eyes pupils so that you can observe a single microscopic image through two eyepieces in stereo vision This greatly helps to reduce fatigue and discomfort during observation While looking through the eyepieces move both eyepieces laterally until the left and right fields of view coincide completely The position of index dot indicates the inter pupiliary distance value Note your interpupillary distance so that it can be quickly referred to in the future This may need when multiple users work with the microscope Fig 18 Adjusting the Diopter Procedure for adjusting the diopter 1 Rotate the right eyepiece to match the markings of your IPD If your IPD is 64 rotate the eyepiece to 64 mark 2 While looking through the right eyepiece with your right eye rotate the coarse and fine adjustment knobs to bring the specimen into focus 3 While looking through the
12. icroscopes base the power cord could melt due to overexposure to heat 6 Always ensure that the grounding terminal of the microscope and that of the wall outlet are properly connected If the unit is not grounded LABOMED can not warrant electrical safety 7 Never allow metallic objects to penetrate the air vents of the microscope frame as this could result in user injury and damage to the microscope 8 After operation of microscope be sure to disconnect power cord from connector socket of the microscope or from the wall power outlet Safety Symbols The following symbols are found on the microscope For optimal use it is recommended that users understand these symbols and always use the equipment as prescribed Explanation This surface has a tendency to heat up and should not be touched unless system has completely cooled down Before use carefully read the instruction manual Improper use could result in injury to the user and or damage to the equipment Warning against risk of electric shock Main switch is ON ol eee Main switch is OFF A Caution If the microscope is used in a manner not specified by this manual the safety of the user may not be warranted In addition the equipment may also suffer damage Always use the equipments as outlined in this instruction manual Getting Started Maintenance and Storage 1 Amicroscope is a precision instrum
13. left eyepiece with your left eye rotate only diopter adjustment ring on the eyepiece until specimen is at its best possible focus Using the Eye Guards When Wearing Eyeglasses Use with the eye guards in the normal folded down position This will prevent the eyeglasses from being scratched When Not Wearing Eyeglasses Extend the folded eye guards outwards direction of the arrow to prevent ambient light from entering into your line of vision R EG 6 Centering the Condenser There is a possibility of having the condenser de centered during shipping itis mandatory to check the centering before observing the specimen as it is required to distribute even light to the specimen For models with Koehler illumination 7 1 Bring the 10x objective 1 inthe front position 2 Move the condenser to the highest point 3 Close the koehler 2 Focus the light spot as show in the illustration below mm 4 Use the centering screws 3 provided on left and right sides of the Fig 21 condenser bracket to bring the light spot to center of the view field g Adjusting the Condenser Position and Aperture Iris Diaphragm The condenser is most often used in the highest position If the observed field of view is not bright enough brightness may be improved by lowering the condenser slightly 1 Rotate the condenser height adj
14. men holder directly by hand This will damage the rotary mechanisms When the specimen holder reaches stop position the rotation force of the X Y knobs become stiff Stop rotating the at this point 14 Cover glass Cover glass This is the glass plate placed on the specimen For optimum optical performance the cover glass thickness which is the distance from its surface to the specimen surface should be 0 17 mm Slide glass Slide glass This glass plate should ideally have a length of 76 mm width of 26 mm 1 mm and thickness between 0 9 and 1 4 mm Fig 15 Vernier Scales These scales allow for easy identification of the specimen s position coordinates making it easy to return to a particular region of interest after scanning the slide Figure 16 1 The horizontal coordinate can be read at position 1 on the specimen holder 2 The vertical coordinate can be read at the index line 2 Fig 16 Adjusting the Focus Focusing Procedure 3 g 1 Rotate the coarse adjustment knob 1 clockwise so that the objective i 3 is as close as possible to the specimen We recommend starting with wp x 10X 1 2 While observing the specimen through the eyepieces slowly rotate the 2 coarse adjustment knob 1 counterclockwise to lower the stage 3 When coarse focusing of the specimen is obtained a blurr ima
15. ng nosepiec Objectives Specimen holde Mechanical stage Aperture iris diaphragm ring O o Coarse and Abbe condenso Ew fine focus knob Filter holder X Y movement control knob Lx 500 STANDARD COMPONENTS After removing your microscope from its packaging make sure that all of the following contents are present Please note that the contents of your microscope may vary as the optional configuration contrasting method or viewing body opted for may not be of the standard configuration highlighted here Binocular viewing tube 30 inclined Eyepieces Observation tube clamping screw x509 Revolving nosepiece Objectives Specimen holder Mechanical stage Aperture iris diaphragm ring gt O o Abbe condensor a Coarse and Filter holder z fine focus knob Moyen 0 Daylight blue filter Paired Eyepieces Allen Wrench 3mm OPTIONAL ACCESSORIES System Diagram of Optional Accessories iVa 5000 iu 7000 Video ada japter1 2 amp 1 3 Digital camera adapter iVu 1500 L L L 2 KS head it aes head is th head WF 10x WF 16x WF 20x scooooesec RP 4x RP ie RP w RP a SL RP 50x i Oil RP k SL Oil RP ii 10x RP ai 20x RP PH iia SL RP PH ii SL Oil 0 O O O RP FL 4x RP FL 10x RP FL 20x RP FL 40x SL RP FL 50x SL Oil RP FL er SL Oil RP Ss Oil
16. nification same magnification LABOMED does not recommend using third party eyepieces in conjunction with LABOMED microscopes 19 1 aaaaaaauuuuuuuuuuuuuuuuuussssl Lx 500 Observation Cause Remedy 12 Objective hits the specimen when The specimen slide is upside down Set the specimen correctly with the an objective is switched to a higher cover glass facing upwards magnification objective The cover glass is too thick Use acover glass with thickness of 0 17mm The stage is raised too high Lower the stage The slide has slipped from the slide Re position the slide in the slide holder holder Slide is of excessive thickness Use slides with thickness between 0 9 and 1 4mm 13 Bulb does not turn On Bulb is not mounted Attach a bulb Bulb is blown Replace the bulb The power cord is unplugged Not firmly Ensure power cord is securilly plugged secured into the box socket wall outlet Fuse is blown Check and replace with live fuse Battery is low Charge battery 14 Bulb blows easily The specified bulb is not used Replace with the specified bulb 280 SPECIFICATIONS 1 Illumination Built in illumination system Halogen 2 Focusing mechanism Stage height adjustment mechanism Fine adjustment scale 0 003um per graduation Fine adjustment stroke 0 3mm per turn Total stroke 12 7mm Co axial coarse and fine focusing on ball d
17. r 1 to detach it 2 Pull up the lamp from the lamp holder amp replace with lamp 3 Replace the lamp house cover Always use the designated bulb Using a bulb other than those specified by LABOMED may lead to a fire hazard and decentering of light Fingerprints or stains on the lamp bulb reduce its life If contamination occurs wipe bulb surface with a cloth slightly moistened with alcohol Fig 10 A caution For Bulb Replacement During Use or Right After Use The bulb lamp socket and areas near these will be extremely hot during and right after use Set the main switch to O OFF disconnect the power cord from the wall outlet and allow the bulb and lamp socket to cool before replacing the bulb with a new bulb of the designated type Cooling time may vary to users discretion Mounting the Daylight Blue Filter This filter modifies the color of observation light into a natural daylight color e Fit the filter 1 into the bottom of the condenser 2 until it clicks into place Figure 11 12 SUMMARY OF BRIGHTFIELD OBSERVATION PROCEDURE Flip the main switch to ON gt gt l Place the specimen on the stage On Off Engage the 10X objective in the light path C Inlet Bring the specimen in focus Adjust the observation tube and eyepieces
18. rive 3 Revolving nosepiece Quintuple positions fixed Reverse angle 4 Observation tube Binocular Trinocular Ergonomic Field number 22 Standard 22 Standard 22 standard Tube tilting angle 30 30 0 25 Interpupillary distance 48 75 48 75 48 75 adjustment range 5 Stage Size 200 x 160mm with mechanical stage Movement range 76 x 50mm Specimen holder Double specimen 6 Condenser Type Fliptop Abbe condenser daylight filter detachable N A 1 25 Aperture iris diaphragm Built in 7 Dimensions amp Weight 301 0mm L x 227 0mm W x 420 mm H 9 kg net 8 Electrical Halogen 6V 30W Bulb life Upto 500 hours 9 Operating environment Indoor use Altitude Max 2000 meters Ambient temperature 5 to 40 C 41 to 104 F Maximum relative humidity 80 for temperature up to 31 C 88 F decreasing linearly through 70 at 34 C 93 F to 50 relative humidity at 40 C 104 F Supply voltage fluctuations Not to exceed 10 of the normal voltage Pollution degree 2 in accordance with IEC60664 Installation Overvoltage category II in accordance with IEC60664 248 www laboamerica com Our policy is one of continuous development Labo America Inc reserves the right to change design and specifications without prior notice UA STEREO
19. se a zig zag motion 2 Cleaning of painted surfaces Avoid the use of any organic solvent e g thinner xylene ether alcohol etc for cleaning of painted surfaces of the instrument Painted surfaces can be cleaned with a very lightly moistened micro fiber cloth Loose dust and other dirt particles can be removed using a soft bristle brush used exclusively for this purpose Lx 500 Lx 500 Binocular Binocular viewing tube 30 inclined Eyepieces Observation tube clamping screw Revolving nosepiece Objectives Specimen holder Mechanical stage Aperture iris diaphragm ring A 0 i Abbe condensor x k Coarse and Filter holder D a fine focus knob X Y movement control knob 5 Lx 500 Trinocular a Trinocular Port NN Eyepieces Trinocular viewing tube 30 inclined Observation tube clamping screw t500 Revolving nosepiece Objectives Specimen holder O Mechanical stage Aperture iris diaphragm ring Z O 0 Abbe condensor A Coarse and Filter holder fine focus knob X Y movement control knob Lx 500 Lx 500 Ergonomic Binocular Eyepiece Ergonomic Binocular viewing tube 0 to 25 inclined S Observation tube clamping screw Lx 509 Revolvi
20. ustment knob 2 to move the condenser to the highest or desired position 2 The aperture iris diaphragm ring 1 has an objective magnification scale 4X 10X 40X 100X Rotate the ring so that the magnification of the objective is clearly visible while using the microscope Rotate the revolving nosepiece 1 so that the objective to be used is in line above the specimen Always use the ribbed grip 1 to rotate the objective nosepiece Fig 23 17 Lx 500 Using the 100X Immersion Objective The designated immersion oil should be in contact with the cover lens of the 100X immersion objective If not the specimen will appear distorted and dull Itis recommended that LABOMED immersion oil is always used Immersion Process 1 Bring the specimen in focus using first the 10x then 40x objective 2 Disengage the 40x cycling towards 100x and place a drop of immersion oil on the center point of the specimen 3 Rotate the revolving nosepiece to engage the immersion objective and rotate the fine adjustment knob to bring the specimen into focus Since air bubbles in the oil will affect the image quality make sure that the oil Is free of bubbles To remove bubbles rotate the revolving nosepiece slightly to agitate the oil 4 The condenser of this microscope manifests the full performance when oil is placed between
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