Lx 400 FLR User Manual
Contents
1. 7777 gt Specimen Fig A The reflected light fluorescent microscope features a dichroic mirror which directs the excitation light through the objective the specimen and provide efficient fluorescent light observation The microscope is used in the study of various cells and organism which get excited in particular wavelengths of incident light Transmittance Reflectance Absorbance 50 0 s Wavelength Crossover Fluorescence Attachment User Guide 1 PACKAGING CONTENTS 10 11 eS Se SS SF Illumination Lamp Housing Sliding Filter Block Blue Green amp Open Power Supply Unit Objective Set 4x 10x 40x 100x Power Cable Connecting Cable UV Rays Shield Immersion Oil Bottle to be used with 100x Mercury VaporLamp UV Ray Shield Fixation Screw Allen Key Fluorescence Attachment User Guide SAFETY INSTRUCTIONS Fluorescence Attachment User Guide Fluorescent attachment is manufactured according to CE safety norms and regulations Fluorescent attachment is intended for use only as prescribed in this manual Make sure the main switch on the power supply unit is set to OFF O before supplying power to the unit The power supply unit contains high voltage components Do not attempt to disassemble the unit when turned ON l To avoid injury do not remove the lamp housing while the lamp is lit Also do not turn the lamp ON while the lamp housing is remov2. LA B O M E D ideas for Vision To ensure proper use of this instrument as well as to avoid injury while operating instrument understanding this manual completely before use is highly recommended www laboamerica com VW lt LEJ lt Oo QO INTRODUCTION The reflected light fluorescent microscope features a dichroic mirror which directs the excitation light through the objective to illuminate the specimen and provide efficient fluorescent light observation This microscope is used in the study of various cells and organisms which get excited in particular wavelengths of incident light PRINCIPLE The dichroic mirror is positioned at an angle of 45 to the optical axis of the incident excitation light The excitation light is reflected towards the objective and other unwanted wavelengths pass through the mirror When the specimen is illuminated by the excitation wavelength it emits a visible longer wavelength The dichroic mirror passes most of this light to the eyepiece while the barrier filter mounted between the dichroic mirror and the eyepiece blocks out unwanted to provide a black background The spectral characteristics of the dichroic mirror when it is positioned at an inclination of 45 to the optical axis as shown in Fig B Observation head Exciter filter rbp Barrier filter Mercury vapor lamp Dichroic mirror Collector Objective Excitation light _ gt Emitted light
3. ed to avoid damaging the instrument Before opening lamp housing for replacement of the lamp set the main switch to OFF O and unplug the lamp housing s connecting cord plug from the output connector on the power supply unit Wait at least 10 minutes or until the lamp housing cools down before any changes are made Do not open the lamp housing while the system is turned ON for any user contact with internal parts may cause injury A used mercury burner should be disposed of in compliance with the ordinances or regulations of your national or local government The manufacturer will not accept any liability for damage caused by any user following unsafe practice of the instrument Avoid using the lamp beyond its service life The mercury lamp seals high pressure gasses If the lamp is used beyond its service life stress may accumulate inside the lamp and in the worst but very rare case the lamp may burst INSTALLATION Unpack all parts from original packaging and verify that all items in packing list are included It is recommended that original packaging is retained for storing attachment parts when not in use 1 Remove UV ray shield from its packing and attach it to the base of the sliding filter block with the thumbscrew provided in the kit Fix the UV rays shield by tightening thumb screw clockwise as shown in Fig 2 Fig 1 St he ad ihe ii are 2 Remove observation head from dovetail adapter and fix sliding filt
4. er block in Fig 2 place of observation head by tightening the screw allen key is provided in kit as shown in Fig 3 Make sure this attachment is firmly fit into the dovetail adapter and there is no tilt or play 3 Replace the observation head on top of the filter block and secure by lightening Fig 3 thumbscrew as shown in Fig 4 Check for proper fitting of observation head into dovetail adapter Fluorescence Attachment User Guide 4 INSTALLATION 4 Remove power supply unit from its packaging and make sure there is no physical damage to connecting terminals or switches 5 Loosen the thumbscrew indicated by the arrow as shown in Fig 6 and gently pull out lamp door from lamp housing 6 To avoid any damage to the mercury vapor lamp a plastic rod is fitted in place of lamp during transit Remove this rod by loosening the two screws indicated by the two arrows shown in Fig 7 7 Mount the mercury vapor lamp in place of plastic rod and tighten the screws to secure the lamp as shown in Fig 8 Insert the thicker end of the mercury lamp into terminal A and insert the thinner end of the lamp into terminal B as shown in Fig 9 8 Avoid touching the lamp in order to prevent leaving finger prints on the glass If there is any residue on the lamp s surface gently wipe it off with soft tissue paper Fluorescence Attachment User Guide HHLI TI Fig 6 Fig 9 INSTALLATION 9 To power
5. luorescence Attachment User Guide 7 TROUBLESHOOTING Optical System 1 The mercury lamp lights up Filter slider is not engaged properly Adjust filter to required setting but field of view remains dark in light path This process is shown on Page 7 2 Image is unclear blurred or a Objectives and or filters are dirty Clean them with tissue lens paper has poor contrast b Field iris diaphragm is not Adjust field iris diaphragm so fully opened that the image circumscribes the field of view 3 Field of view is vignetted or a Objective is not engaged properly Be sure when rotating nosepiece itis not evenly illuminated in light path that objective clicks into position b Filter slider is not engaged properly Engage required filter c Mercury lamp is not centered Center mercury lamp correctly This process is shown on Page 7 d Beam focus deviates from correct Adjust the condenser knob This process is shown on Page 7 4 Mercury lamp is not illuminating Some mercury lamps may not ignite Press the Trigger button provided after switching power supply ON the first time the power supply is on the front panel of power supply turned ON unit and wait for lamp to ignite Electrical System 1 Lamp housing Main switch a Power cord is not connected Make sure power cord is firmly indicator does not light up properly in place b Thefuseis blown Replace with fuse of same type 2 Main switch indicator lights up a Connector cable is
6. not engaged Verify connection is secure yet mercury lamp remains OFF indicated on Page 5 3 Light demonstrates partial a Sufficient time has not been Allow atleast 5 minutes after flickering allowed for the lamp to fully turning power supply ON for the illuminate lamp to fully illuminate If flickering persist press Trigger button b Lamp s service life has expired Replace the mercury lamp part 3127010 Fluorescence Attachment User Guide 8 TECHNICAL SPECIFICATIONS Type Observation modes Lamp type Supply Voltage Operating environment Reflected Fluorescent illumination based on slide switching of dichroic mirror cubes B excitation G excitation and Open field O HBO 100 W high pressure Mercury vapor lamp 110V AC 60 Hz 220V AC 50Hz Indoor use Ambient temperature 5 C to 40 C Maximum relative humidity 80 at 40 C FILTER SPECIFICATIONS B excitation Excitation filter Dichroic mirror Barrier filter Recommended FluorescentDye Alternative for Fluors G excitation Excitation filter Dichroic mirror Barrier filter Recommended FluorescentDye Alternative for Fluors Fluorescence Attachment User Guide Transmission 90 at wavelength 420 to 480 nm Transmission 0 at wavelength 485 nm onwards Transmission 93 at wavelength 540 nm onwards Transmission 0 at wavelength 400 to 530 nm Transmission 95 at wavelength 535 nm onwards Transmission 0 at wavelength 400 to 500 nm Ac
7. ridine Orange both DNA amp RNA 5 Carboxyfluorescein 5 FAM 5 Carboxytetramethylrhodamine 5 TAMRA 5 FAM 5 C arboxyfluorescein Transmission 85 atwavelength 490 to 525 nm Transmission 0 atwavelength 530 nm onwards Transmission 90 at wavelength 600 nm onwards Transmission 0 atwavelength 400 to 595 nm Transmission 92 at wavelength 615 nm onwards Transmission 0 atwavelength 400 to 575 nm Acridine Red FM 5 95 Ethidium Bromide LDS 751 DNA Nitrobenzoxadidole www laboamerica com Our policy is one of continuous development Labo America Inc reserves the right to change design and specifications without prior notice
8. ugh time to fully ignite in order to achieve a fully illuminated centering beam When the lamp is fully illuminated a beam of light is visible through the beam centering window arrow 1 Fig 13 provided on the front side of the sliding filter block Adjust the sharpness of this beam by adjusting the condenser knob arrow 2 Fig 13 until a focused beam appears in the window To center this beam of light in the window two calibrating knobs are located on the lamp housing as indicated by the two arrows in Fig 14 By adjusting these two knobs the focused beam will shift Adjust the beam position to the center of the window as shown in Fig 14 Fig 14 OBSERVING A SPECIMEN mM Turn the power supply ON Allow the lamp at least 5 minutes to fully illuminate for optimal use m Place the specimen slide on stage Select the excitation light by adjusting sliding filter block as follows For G excitation Pull filter sliding block to its outermost position For B excitation Push filter sliding block in to its innermost position For O light non excited light Set filter sliding block to its middle position Observe the specimen slide through the microscope s eyepieces m When no fluorescent light is required turn the attachment s power supply OFF and set the sliding filter block to its middle neutral position To view the specimen in normal condition under the microscope s light turn the microscope ON Fig 15 F
9. up the fluorescent attachment remove the connecting cable from its packaging and insert the three pin female connector to the input terminal of the lamp housing door as shown in Fig 10 Fig 10 10 Next connect the other female end of the connector cable into the male output terminal onthe rear panel of the power supply unit as shown in Fig 11 Fig 11 12 Finally connect the power cable from the appropriate power outlet either 110V or 220V to the AC inlet socket on the rear panel of the power supply as shown in Fig 12 Fig 12 MAINTAINING OPERATING LIFE E Ensure that the power supply unit has been connected to proper power supply voltage and line frequency E Set the main switch on the power supply unit to After the lamp is ignited at least 5 minutes are required before the light stabilizes M To avoid shortening the power supply unit s operating life avoid turning power ON while the lamp is not mounted E Avoid turning power ON and OFF within short intervals for this may reduce working life of the power supply E After the lamp is turned OFF it should not be re ignited before the mercury vapors cool and condense Wait approximately 10 minutes before turning power to the fluorescent attachment ON again Fluorescence Attachment User Guide 6 CENTERING THE LIGHT BEAM First allow the mercury vapor lamp at least five minutes to fully illuminate for optimal use it is very important to allow the system eno
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