Wave User Guide 15.3 版
Contents
1. PH Seahorse Bioscience Wave User Guide FEF LB FE EH EE O E E EA DAY EA AE E e DE e DE AA Y AA e DA AA O AO et Ae O 40 4 40 4 4 9 4 4 5 FABER Ee E A AE RIS RR Powder 5682. Data eahorse Bioscience Wave User Guide ee E E E E o E E E E E O E E E E E E E E E E o o E O E E E E a E E E E a E E E E E E o 4 4404 02900040 4 0051 14404 4 40 044 4004094 1 4 0 6040000000 41 44 4 XFp HIRE SH Hee HE 37C gt XFp Wave gt Usb XFp Template XFe24 XFeo6 Bn Start Mito Stress Test Glycolysis Stress Test or FCCP Titration i cell Characterization 1 FOCP titration Cell Characterization 2 Cell Mito E Giycolysis Stress Test Cell Mito Stress Test Acute Injection Coll Mito Stress Test Glycolys s Stress Test acute injection Gtycolysts Stress Test Hb FE AX Protocol FE gt RENA Start Assay Seahorse Bioscie
3. Equilibrate 12 min 2 min Mix 2 min Wait Mix Wait Measure 3min 2min 3min Mix Wait Measure Loop gt ERE ARE gt AERA A AS RATE AAA gt S Injection ABCD Oligomycin FCCP Antimycin Rotenone glucose and 2 DG ABLAR TARA Loop Mito Stress Test Glycolysis Stress Test Protocol Calibration Equilibrate Mix Wait Measure
4. XFp Ag amp H JE o Cell Seeding amp Ee ay AG EC OY om AR IEA 8ouL 80 ul cells I Seahorse Bioscience Wave User Guide HEBE EA E DD AAA DY AAA AAA eee AO A DAA DEA AO AA A A eet ERA Ze on a BR i BRT AS gt AT RR RAG ae PE ARTE FR a gt ERAS RRIF PE Sm HAL I Ze da BR BR ET ER Coating Plate Coating N REAR Coating material Matrigel RR E ta He Ab Mt 89 Coating material poly L Lysine fibronectin collagen E 48 TH ESTE MA RZ 549 Cell Tak Materials 1 Cell Tak Cell and Tissue Adhesive Corning 354240 He A Mytilus edulis Dy BAS FA AR GG Bt m JO e 2 NaHCO3
5. XF24 500uL gt XF96 XFp 18ouIL Ht A ARIK E37 C non COz2 se a b 4 on a scale of 9 Zero Breaking E 450rpm 450Ipm d 650rpm 650rpm E Je Fe ta Fe AA http www seahorsebio com learning cell line php Cell Type Research Area Please select one Please select one AF Assay Author Please select one Reset Fields Seahorse Bioscience Wave User Guide HEBE EA E AE eee
6. Media 49 FAA RE IA 200mL LA LAY RR gt eahorse Bioscience Wave User Guide FEF LB IS ERA 4400 424 4 4 O 4 4 44 04 44 8 4 4D 4 4 8 4 4 4 8 48 4 4 4 04 9 4 0 4 40 4 4 40 4 amp 8 Mito Stress Test Kit Oligomycin FCCP amp Rotenone Antimyin A 1 BREA LARS RADA gt RAAR DMSO 25k O LAF RJAR By gt Volume of Assay Medium Final Concentration Oligomycin 630 uL 100 uM 540 uL Oligomycin Ay B 2h oY dip Hi Final conc 1IUM 10 uM HAE BFR o FCCP Final conc amp 4 2 1 0 5 0 25 uM gt Final conc 10 447 EMAIL RRE o Rotenone Antimycin A Final conc 4 0 5 uM 5 uM HAGE AA o 2 DMOS
7. 2 Step Cell Seeding 100UL culture medium YY gt 1 5 a AAA gt HEAR A 150UL 49 FY ED Ho RATA 150 uL of medium 1 5 hrs incubation Seahorse Bioscience Wave User Guide L4LB 4 p 2 0686 1 1 088U6464 1 4 4 4 E 4 4 94 900404 4 4 0 4 4 4 4 4 04 440 40 4 4 4 04 9 40 4 40 404 4 074 4 004 4 4 AFoo XAFeg6 amp XFp 0 106 cm gt IZS 96 384 well plate cell density o 8 H FA 1 6 X FA well Blank Well XF96 amp XFeg6 A1 A12 H1 H12
8. pH SIA Sodium Bicarbonate or HEPES 2 CO 223444 pH Sodium Bicarbonate or HEPES powder sodium bicarbonate gt Sodium pyruvate glutamine antibiotic 2 Albumin pH 4 A gt 2 Starvation pH 7 4 7 4 pipetman 5 10UL Filter 4
9. Seahorse Bioscience Wave User Guide DE 0004 4 4 44 4 40404 040001514 44 044 44 04 004 044 40 4 4 4 04 9400404000 4 4044 48 44 4040404 414 4 4 04 4 z End Assay Wizard ERR SERS Start lt Save Directory Data Save Name Strat ABORI Guest Seahorse XF24 Current File Experiment is Ready To Start Status IES My Templates Notes Project Name Protocol Start lil 1 Calibrate Probes Je 2 Equilibrate 2 3 Loop 4 times E c Program Files Seahorse Bioscience XFReader24 un 4 1 2 3 4 Mix for 3 Min 0 sec 5 Time Delay of 2 Min 0 sec Un 6 1 2 3 4 Measure for 3 Min 0 sec Atm Pressure 760 Save Name 7 Loop End 8 Inject Port A 9 Loop 3 times yn 10 5 6 7 Mix for 3 Min 0 sec 11 Time Delay of 2 Min 0 sec un 12 5 6 7 Measure for 3 Min 0 sec
10. 10 ABCD 123456 gt XF24 A FAB AFAC ALAD XFez4 of A AEB B ALAC AFA D w 0 Gs O XF24 XFe24 Tip HAGA THB BREA 9 ABERE TO gt BRED Seahorse Bioscience Wave User Guide L4LB 4 p 2 0686 1 1 088U6464 1 4 4 4 E 4 4 94 900404 4 4 0 4 4 4 4 4 04 440 40 4 4 4 04 9 40 4 40 404 4 074 4 004 4 4 XAF96 XFeg6 amp XFp HR AG 4S Ht HEH A 180UL gt ZAA ARE PO AIRES RIE A MARA A 20 ul 22 ul 25 ul amp 27 ulL 10 2 ABCDEFGH 123456789101112 ze E 4 Je Constant Concentration f Starting well volume A B D 180 uL assay medium Vol Conc 20 HL 10X 22 uL 10X 25 uL 10X XFo6 XFeo6 XF 2f uL 10X XFg6 XFeg6 HA By BF FG LE A
11. Non Mito Respiration ROS XF cell mito stress test kit XF24 XF96 XFe24 XFeg6 XFp cell mito stress test kit XFp only E Py Oligomycin FCCP Rotenone Antimycin A 6 10 BER eahorse Bioscience Wave User Guide AAA AA ALA AAA AAA AAA AAA AAA A AA AAA A EA A A AA etter 4 B Glycolysis Stress Test XF Glycolysis St Test Profil XF Glycolysis Stress Test Profile ea e Glucose Oligomycin 2 DG E Glycolysis Measurement rate of glycolytic process Glycolytic Capacity Maximum response to glycolytic demand from stress ECAR mpH min E Glycolytic Reserve Reserve capacity available to utilize glycolysis beyond the basal rate 0 10 20 30 40 BU 60 70 80 90 100 TIME minutes Glycolytic Function Glycolysis no glucose glucose Glycolytic Capacity ATP Glycolytic Reserve Non glycolytic Acidification
12. EA un Background Q save Group Definitions Plate Map Instrument Protocol Review and R ee E cr efe Aas gt Remove Duplicate Generate Groups Mito Stress Test Y No Pretreatments ee ae ZF DMEM y ese C2C12 y A 4 Injection Strategies 1 Injection Condition Mito Stress Test pools E gt EE Mito Stress Test Pretreatments Mito Stress Test No Pretreatments v E DMEM v ee C2C12 Assay Media A Ba C aD ese Cell T ero Eee e Cell Type Mito Stress Test v x No Pretreatments v Compound BZ DMEM y es C2C12 M Compound Final Concentration Oligomycin 1 fam ESE Mito Stress Test y No Pretreatments E DMEM v ee C2C12 Seahorse Bioscience Wave User Guide Add HF AMAA 3 49 Compound Pre treatment Media Cell Plate Map ZE 4814 18 A Ea ee BHAA AA gt BLA 3288 72 He 0111 E 5 Background well Background Wave 2 2 0 Q save Group Definitions Plate Map Instrument Protocol Review and Run af Add Remove Duplicate Down Up Em Background MN ci Mito Stress Test v y No Pretreatments ZB DMEM v ee C2C12 X BE Exp1 Mito Stress Test y No Pretreatments E DMEM v ee C2C12 X E p2 Mito Stress Test v y N
13. XF glycolysis stress test kit 18 FA XF24 XF96 XFe24 XFeg6 XFp glycolysis stress test kit XFp only glucose oligomcycin amp 2 DG 6 BER eahorse Bioscience Wave User Guide C Fatty Acid Oxidation Oligomycin FCCP 8 Rotsnone Mito Stress Test gt BSA conjugated Palmitate 40 dep i H etomoxir o 0 10 20 30 40 50 60 TIME minutes XF Palmitate BSA FAO Substrate XF24 XF96 XFe24 XFeg6 BSA amp BSA conjugated Palmitate 3 EER Seahorse Bioscience Wave User Guide plasma membrane permeabilizer PMP J 2 4a Je gt ati Oligo FCCP AA ER RETA AR EA ADP succinate AJ RE SR RE Y AE PAE e OCR pMolesimin XF Plasma Membrane Permeabilizer XF24 XF96 XFe24 XFeg6 XFp A lt gt PMP 10 eahorse Bioscience Wave User Guide FEF ESE eee AE
14. Oligomycin FCCP amp Rotenone Antimyin A 126 144 54 UL DMS EJ A th Bye EA 0 5 mM Stock ARRE Fincal Conc 10 PREMIA RENA gt lt Seahorse Bioscience Wave User Guide A A E A A A A A A E A A A E A E A E ee Glycolysis Stress Test Kit Oligomycin 4924 NB EIRA ARA A Glucose powder 2 DG powder gt RE REA No Glucose HALA ER By Volume of Assay Medium Final Stock Concentration a 7 3000 ul 100 mM Oligomycin 720 uL 100 uM 3000 uL 500 mM Glucose Final conc 10 mM HAA te a h A hE EAT 10X o Oligomycin Final conc 1UM 10UM HAGE BFF o 2 DG 2 Deoxy Glucose Glucose RL ME A dl Aen Glucose Final conc 5o mM 1oX Seahorse Bioscience Wave User Guide LE Seahorse Stress Test Dilution Calculator APP Seahorse Bioscience 24 14 f5 4 ot RH a Svs ics ee CO PERIANA Lo x E FINAL WELL EC oo PORT A STOCK 0 T e VOL TO PREPARE 8 2 44 84H 0 pL Ygs VOL S
15. DEA AY AA AE AE e OE terete AY EA Ae DA AA O AO e E Ae 074 404 4 40 4 4 4 4 4 4 XF24 amp XFe24 0 275cm 96 well plate 96 well plate 2 4 FA well o Blank Well Al B4 C3 D6
16. Mix Wait Measure 141min Seahorse Bioscience Wave User Guide General Cells Media 8 PPR Compounds Back Correction Groups amp Labels Display End Current Protocol Duration fm o min Available commands Protocol Start y y Wait Ar gt ll 1 Calibrate Probes jt 2 Equilibrate wo 3 Loop 4times Ut 4 1 2 3 4 Mix for 3 Min 0 sec 5 Time Delay of 2 Min 0 sec 6 1 2 3 4 Measure for 3 Min 0 sec ii 7 Loop End 8 Inject Port A ea ne juain ang aas IA sovo 10 5 6 7 Mix for 3 Min 0 sec S 11 Time Delay of 2 Min 0 sec 12 5 6 7 Measure for 3 Min 0 sec End n y 14 Inject Port B 15 Loop 3 times 16 8 9 10 Mix for 3 Min 0 sec 17 Time Delay of 2 Min 0 sec uu 18 8 9 10 Measure for 3 Min 0 sec 19 Loop End 20 Inject Port C DA l ann 2 timac Display Data EMMA gt Bee wR tee End End Wizard Save Template Save Template
17. 2oop Pipetman gt Tip 1 4 4G IES Well gt its Suction Wash gt ju Boul Well Blank Well Seahorse Bioscience Wave User Guide FEF BIS EH AY EE 44000 42 8 4 O 4 4 44 04 44 0 4 4D 4 4 0 4 4 4 0 48 4 4 0 4 9 4 0 4 40 4 4 40 4 4 04 44 4 Prep Station 200mL gt SEE 37 C Prep Station eal E 37 C gt AE Media 69 HET DARA 200mL 70 200mL a he HL i Prep Station gt 7 Media change 47 A gt Do Prime amp Do Rinse LS e bk Final Volume 4 180 UL Ri Columns All gt 4 2732 A dm et PRAL H1 RIAL T R gt HEF Start PEE EEES FE ARALAR 37C CO2 Prep Station
18. SSE AER Seahorse Bioscience Ky EE TTD NO AGA A TRE O CELL BIO BIOTECHNOLOGY Wave User Guide 49 42 4 4 4004 400 04006 10141114241 0 4241040110040050404 415 4011 4 01 00 0 000 1 1 00 4411 0 40 4 40 0141057 0090 4404 4 4004 44 40 404 44444 4 B k DOE Assay ROA ea NOAE 3 1 RN 4 2 ev 5 3 Ne 6 a Mito Stress Test b Glycolysis Stress Test c Fatty Acid Oxidation d Complex protein Activity Substrate Utilization 4 Ne 10 E e as AMM o 16 6 ee 19 Day Before Assay Day of Assay E LANE peda 20 8 EE EH AI coccccnccncnnccncnnncnnnnocnnnnocnnnonononnccnnnoccnanncccanononnns 22 DA RIRN an 25 WO oe A E 27 Seahorse Bioscience Basic Procedure XF Assay Roadmap After Your Assay After Your Assay 4 Day of Assay Day Prior to Assay Y 3 SS Planning Your Assay e Prepare assay medium e Wash cells gt e Prepare assay gt compounds e Load cartridge with compounds e Design load instrument protocol e Run assay Seahorse Bioscience Wave User Guide 041044 4 4 44 E 44 44 04 004 044 44004 4 4 4 04
19. 8 44 404044 4414 et 1 XF24 XF096 Seahorse Bioscience Wave User Guide 4 444 400 4 4 4 4 4404 00400015414 4 444 4 0400400444408 44 4 04 44 404044 4 1414 et 2 FluxPak Series PI 44a faust A RARA amp XF24 XF24 FluxPak XFo96 XFg6 FluxPak XFe24 XFe24 FluxPak XFeg6 XFeo6 FluxPak XF FluxPaks XFp XFp FluxPak XFp FluxPaks XF24 Islet FluxPak XFeg6 Spheroid FluxPak XF24 XFe24 only XFeg6 only AMERO AENA Sphere HA Seahorse Bioscience Wave User Guide 3 A Mito Stress Test Mitochondrial Respiration P Oli i FCCP Anti in A XF Cell Mito Stress Test Profile In BN E Basal Respiration E ATP Production m Proton Leak Maximal Respiration E Spare Respiratory Capacity OCR pMoles min Hi Non mitochondrial Respiration 0 10 20 30 40 50 60 70 80 90 100 110 TIME minutes Basal Respiration 1 4p 92 7 3k G8 HK AS BF OD FE A E ATP Production 2 45 4298 A 2 DP BA Aw E ATP Proton Leak R fe RL oe ae ES ROY EEE gt A By MMP Maximal Respiration Spare Capacity
20. gt SEAR pH Seahorse Bioscience Wave User Guide FEF LB IFE ERA EE 4400 44 44 O 4 4 44 0 4 44 0 4 4D 4 4 0 4 49 4 0 44 4 4 0 4 9 4 0 4 40 4 4 4 4 4 Liquid Liquid Sodium Bicarbonate or HEPES gt Powder HARE gt ERA Pa gt EF REA RARA RE Liquid gt MATL HMA powder 334 E A A A IE E ex DMEM MEM or RPMI 443 Glucose Sodium pyruvate glutamine SDP insulin AAR Rigs AA LA RA RIA gt ERA i 2 AJ RMR E H Seahorse Bioscience Wave User Guide XF Base Medium DMEM no g
21. 13 Loop End 14 Inject Port B 15 Loop 3 times Un 16 8 9 10 Mix for 3 Min 0 sec 3 17 Time Delay of 2 Min 0 sec Unselect EY Y Measure Inject Wave User Guide A A A E A A A IS A A A A E A E A A A E A E E Start 2 8 BA AICHE PIR gt ARE PRIR Et A Tik Continue 30 min Data eahorse Bioscience Wave User Guide HE LB IS EH EE 44000 424 4 4 O 4 4 494 04 44 8 4 4D 4 4 0 4 49 4 0 48 4 4 04 9 4 0 4 40 4 4 40 4 XFe24 amp XFeg6 pA mt Wave Software amp BP Be AAA 3B LE o Offline Offline 4 4 Connected gt 37 ae on Heater ON Wave 2 2 NEW ASSAY Design Blank I
22. EE TES Hydro Booster 45 gt XAF96 XFeg6 Utility plate 96 14 well 200 ul 37 C CO overnight XA Fp Utility plate E 8 44 well 2 A 200 uL BEA 12 49 9 8 0148 48 gt 400 UL CO overnight A A a b A re Ml 9 q 9 PP Seahorse Bioscience Wave User Guide FEF 4B 4 4 p 0 0 0060b6 0 000 1 4 4 4 4 4 49490 440 40 4 4 4 04 440 408 4 4 4 04 9 4 0 4 40 44 4 4 amp tiet amp Ft E 7 37C 37C CO XF24 XFe24 1000p Pipetman gt Tip 5415 42 Well gt Hs RAK Suction 5 RA RZ RAZA Wash 675 UL RARAS MARA RAE AMELIA Well gt Blank Well XAF96 XFeg6 amp XFp
23. 71 2 BJ AR EAU Tip 4A 444 Bh 48 E BEN gt VET E EE MA E E E o Seahorse Bioscience e E 44 2444549004944 4444 444 0400 0 44 40 4 44 404 040400404408 4404940040 01101 1444 004 48 10 EMS 37C Overnight XF24 amp XFQ6 a 1 M x Seahorse XF24 or 96 A Software 2 25 Standard A gt HEAR IMA AAA XF_Software 4 Standard Seahorse Apps XF Cell Stress Test Kit VW Start App About this screen Seahorse Bioscience W U G id E E EEEE AAA AAA AAA AAA AAA AEREA A AAA AAN eee AAA AAA AAA AAA AAA AAA AAA AA A 3 Seahorse Guest Guest Seahorse XF24 Current File E resurs 3 Files 2 Help Status Compound Desc Protocol Info Settings b New Plate r E Groups Media a ax Y TA E Open Assay Wizar Instrument rep Station Logout RELIES en Id E E B4B th A BATA US ERAN RARE b Start HILL A P te Be Assay Wizard d Logout Seahorse Bioscience Wave User Guide Assay Wizard Cells Media amp PPR Compounds Back Correcti
24. Sigma S5761 42omg NaHCO3 4 50ml pH 8 0 gt 10 22 um 4 C 0 1M NaHCO3 3 Sterile Water Procedure 1 vACell Tak ke IF 45 fx Bt AE Cell Tak 3 5ug 2 54 MG ML XF24 13 uL Cell Tak 1 5ml NaHCO3 Well 50 ul gt XFo6 17uL Cell Tak 2 0ml NaHCO3 well 20 UL XFp 1 5uL Cell Tak 150UL NaHCO3 well 20 UL plate Laminar Flow i A gt AF r WF 20min 5 20min HAS E gt to A2R K XF2440 A200uL XFo6 XFp 1oouL BKK NaHCO3 gt 2omin fq fra A ta Fe Bb E e Seahorse Bioscience Wave User Guide L4B 4 1 6 00 0 841 02 4 4 4 9 4 4 94 80404 4 4 0 4 4 44 04 440 40 4 4 4 04 9 40 4 400404 4 40 0 ete E 2 45M 48 M8 Laminar Flow 37 C_ cell tak XF24 100UL gt XFo6 XFp 5ouL 30min
25. TOCK SOLUTION TH2 S Stock Hx 2 gt 4844 4 2 47 pH VOL ASSAY MEDIUM 4 El El OBE Seahorse Bioscience APP Google Play https play google com store apps details id air com seahorsebio calcul ator amp hl en Tunes https itunes apple com us artist seahorse bioscience id956653634 Seahorse Bioscience Wave User Guide FEF LB IESEAH AS AE E E EA DAY EA A E e DE e DE AO Y EA Ae DA AA O A Y E Ae BD 940 4 40 4 4 40 4 amp 4 4 9 it RAE RENE A RA pH FAT ARS MAB BIB A Final conc 4 10 Well XF24 XFe24 Lactose ARM A 675uUL 75UL 85UL 95suLR amp liooUuUL
26. eee AE AA A AA DY AAA AAA A ete eet A Y E AAA DAA AA OEA AO AA A A Q1 24 Cell Density Test Q2 RAR E He Transfection EZARRIA AA RRR ROR RO BVERUALEBRSR S Cell Density Test Vector only 4a Vector only wild type Vector Vector 03
27. lucose no sodium bicarbonate amp HEPES pH 7 4 1L XF Assay Medium DMEM no glucose 2mM GlutaMAX no sodium bicarbonate HEPES pH 7 4 1L Sn Mg os MgSO osmm OB mM Can ascal 18mM 18mm NaCl 143mM 143mM kd 5 4mM 5 4mM NaH PO 0 19mM 0 19mM PhenolRed 3mgL 3mgL Lmlutamine 0OmM omM Glucose omM omwm Sodium Pyruvate OmM Omm Sodium Bicarbonate NaHCO3 Omm__ 0OmM k Seahorse Bioscience Wave User Guide FEF L4B 4 4 p 686 1 0 81444 4 4 4 4 4 94904 4 4 O E 40 4 4 4 04 440 48 4 4 4 04 94944 490404 4 04 4 0 44 4 6 gt BR 37 C CO overnight 37 C CO CO parafilm BEBA AM BE gt XF24 Utility plate 24 18 well 0 A mL REX gt mB 37C CO AER overnight Bp XFe24 utility plate 24 48 well 1mL Hydro Booster E TOM BAER P KAT EAR gt
28. nce Wave User Guide O 544 Start Assay Load Cartridge HRT Continue RA 20 min Data Please place the cartridge with its utility plate on the tray Make sure the cartridge hd is off Touch Continue to proceed with the assay or Cancel to exit the run Seahorse Bioscience Corporate Headquarters European Headquarters Asia Pacific Headquarters Seahorse Bioscience Inc Seahorse Bioscience Europe Seahorse Bioscience 16 Esquire Road North Symbion Science Park Asia 199 Guo Shou Jing Billerica MA 01862 USA Fruebjergvej 3 Road Suite 207 Pudong 1 978 671 1600 2100 Copenhagen Denmark Shanghai 201203 CN 45 31 36 98 78 0086 21 33901768 vue Seahorse Bioscience se gt o CELL BIO BIOTECHNOLOGY Tel 02 2785 5860 Fax 02 2785 7237 Web www cell bio com tw Mail contact cellbio Wgmail com Add
29. o Pretreatments E DMEM y ee C2C12 Instrument Protocol Calibrate Equilibrate ERE o Ds eeeeo New Design El Dense Q save Group Definitions Plate Map Instrument Protocol Review and Run d Measure 5 l Injection Custom Remove in Left 0 Basal 4 Measurement Cycles Total Time 01 56 00 x CEET E initialization Calibrate The XF always performs calibration to make sure EJ P Oligomycin Ea Si Fcce Edit Measurement Details measurements are accurate Cycles Mix Wait Measure A A A Y Equilibrate 03 00 02 00 03 00 Equilibration occurs after Calibration and is v v v v recommended which is why it s checked 00 32 00 Seahorse Bioscience Select Ports gt clp W Measure after Injection 3 Measurement Cycles A Edit Measurement Details Cycles Mix Wait Measure b A A A Yo Mas Y e v Group Summary 00 24 00 v Select D Ports gt ellp Measure after Injection 3 Measurement Cycles A Edit Measurement Details Cycles Mix Y Group Summary 00 24 00 Wait Measure bh A A 03 00 02 00 03 00 03 00 02 00 03 00 Wo Ya Y Select Allg dp Ports Y Measure after Injection 3 Measurement Cycles Edit Measurement Details Cycles Mix Wai
30. on Groups amp Labels Protocol Display End Project Information Notes Assay Name No Name Project Name Project Number Principal Investigator Instrument Operator Department Result File Name General 2 4 FRE EBARA AS SRAM WA wy BEE Lord from Template P r 4 Be aR E AAA Template Data Cells Media amp PPR 224 13418 A medium Compounds 2 24 8 A 2 fe gt REAM ES BGM e S Seahorse Bioscience DE S E E SE IE SE SE SE S E E E E E E E E E E E E E E E E E E E i E E E E O E E E O E E O E E E E E E E E E O E E O O E E E E E E E E E E E E E E E Back Correction Do background correction 4 4k Xk 32 gt 40 AR Background well se
31. so Mito template Name Blank Modified 11 6 2014 Author Seahorse Bioscience Description Blank design template for an XFp Project Name Project Number Investigator a New 3 416 HERE gt AAA IR E Open 725224 FA Browse AiL E Sr Data Catalog Compound Media amp Cells Options Help J 4 hi AUR RYE Ft gt SETTER ES Ml o Seahorse Bioscience Wave User Guide b Instruments XFe24 XFe24 Hypoxia mode XFeg6 XFeg96 Hypoxia mode XFp TOBIN E c Template J 25 Blank ee o d Design Import Export amp Delete Template Template a ih PEA BRA gt REME S RAN Template 252 Blank gt 25 8 Design Wave 220 New Design EJ boss E save Group Definitions Group Definitions Plate Map Instrument Protocol Review and Run Add Remove Duplicate 3 Down P Up
32. t Measure A bh A A 03 00 02 00 03 00 Ya We Ss a Group Summary 00 24 00 Wave User Guide EF BIS ERA AE E E DEA DAY EA AE e OE e DE AO AY E AA e DA AA O A E Ae 74H 4 044 4 40 4 4 40 4 Mix Wait Measure gt FRBRERMASEP RAE Cycle Injection Injection ABCD Calibration lt o Review and Run A EA RO E wy 253 4 7 Start Run Start Run XFe24 Hydro booster Continue 20 min
33. x Eo 32 ey Bee AA ag ek eB EZE AJALE General Cells Media amp PPR Compounds Groups amp Labels Protocol Display End Wells used for background correction Do background correction Label for background wells Form a group with background wells Label for background group Back Corr Groups amp Labels 2524 Group info Group Name General Cells Media amp PPR Compounds Back Correction Protocol Display End Well Labels Group J ES hl Ja Load Groups Save Groups O O O O O Seahorse Bioscience Wave User Guide Ed EA AE DEA AE AAA DY AAA AAA A etre eet A AA 4 DAA DEA AO AA A amp Protocol R THRE A ARH SEAS ARA ar ERAN Start and End RE Calibrate Probe 25 min Duration
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