User Manual Micro-Volumn Spectrophotometer For Nucleic Acid
Contents
1. Enter the spot behind the result will brighten and press Print to start print If user want to print more than one result just need choose your target ones spots should be brightened When users operate by mouse or touch pen Scroll through the table using the slider bar on the right of the window click the spot behind results to make spot brightened and click Print to start print 14 13 After typing in a file name by keyboard or mouse press Enter on keyboard to finish typing Press Enter again or click OK to save file See below Notes When type characters by keyboard must make sure the previous one is black Or press Esc Or click return to quit Save As sro D ne o Sair M CANCEL GN SAVE FILE 5 4 Protein A280 measurement 5 4 1 Protein A280 measures the protein s absorbance at 280nm and calculates the concentration mg ml Like the Nucleic Acid mode the Protein A280 records 10mm equivalent data Choose Protein on main interface enter in interface of Protein A280 measurement as below iAbs OBSA OlgG OLysozyme 24 Protein Measure Program oe Measure 5 3 3 Create a blanking by using suitable solution Blank solution is normally solvent to dissolve the targeting molecular and it needs to be the same with the samples in PH and ionic concentration Load a 2ul blank sample onto the lower pedestal and lower the upper pedestal2. the icon Fa will brighten See bellows Spectral Resolution lt 3nm FWHM Hg 253 7nm LJ Absorbance Precision 0 002Abs 1mm path length test tet test4 txt Absorbance Range 0 02 80 equivalent 10mm Detection Concentration 10ng uldsDNA 4 000ng ul Range dsDNA prod tet tests txt test4 txt FLASH_DATA Acid i Then press Enter to delete Save Type 64M flash 2GB SD Card Or after choosing file and click icon to delete by mouse or 19 Press key Tab when View type turn red Press down direction key to choose file you need Or click by mouse or by touch pen to choose see below History Vew scope E he cid e CANCEL OK Welcom to NANO 200 Press Enter Touch OK or click OK by mouse to see see below Or can press Esc Touch CANCEL or click CANCEL by mouse WUYUYL Bet prod t lt t tests txt test4 txt tests txt 4 FLASH_DATA Acid Choose files by up and down direction keys turn pages by left and right direction keys User can click file name by mouse or touch pen Click page No under the table can jump to the any page you want Or click and to change pages After you chose a file the file name will be turn red press Tab to choose to make it brightened as below 3 Basic Operation 3 1 Structure description Upper pedestal Lower pedestal Panel Touch screen Printer SD Card
3. RS232 Connector USB to mouse USB output Power connector Switch 3 2 Sample size requirements 5 5 check history record Although sample size is not critical it is essential that the 5 5 1 Choose History on the main interface enter into the complete liquid column can be formed between the upper history interface as below measurement pedestal and lower measurement pedestal to make sure the precision of the measurement History lt is best to use a precision pipettor O 2ul with precision tips to assure the precision of the sampling If users are unsure about View scope v sample characteristics or pipettor accuracy a 2ul sample is x recommended u 3 3 Basic use for the pedestal With the upper pedestal open pipette the sample 2ul onto the lower pedestal Elng 5 S CANCEL OK Lower pedestal Welcom to NANO 200 Press Tab to make View scope turn red then press down direction key to choose file position also can click Ww by mouse or touch pen Notes when spot behind the SD is gray means SD card has not been active or without SD card This moment user has to choose FLASH as below History Lower the sampling arm the sample column is automatically drawn between the upper and lower measurement pedestals an Flash Then the measurement initiates vee 5 5 CANCEL OR Welcom to NANO 200 Or choose position by clicking icon
4. plastic paper to prevent from dust Clean the pedestal by a clean soft cloth stained by alcohol Clean smutches on the Instrument by soft cloth stained with cleaning cream 7 Failure analysis and handling Phenomenon Processing Procedure Equipment doesn t Work after power on Touch screen not responsive Measure result not accurate Possible Causes No power Check power Switch failure Change switch Adaptor failure Contact seller Fixed position deviates Screen failure Fix again Contact seller Make sample to a complete column Clean the pedestal and blank calibrate Sample drop not complete Pedestal polluted 22 21 5 5 2 When user open one file the datas in for file will display as follow Dsdna o Ssdna O Rna FLASH_DATA Acid 5 09 Return to previous step by pressing key Esc or click Return by mouse or by touch pen Read all data by press direction key A and key y on keyboard or click scroll Column on the screen On the bottom of the screen can see measurement type When user need to print one result choose one of the results by pressing key then press Enter The point behind the result you choose will brighten Get printing result by clicking Print User also can choose more than one User can operate by mouse or touch screen to choose results which need print Click points behind results by mouse or touch
5. sg using mouse or by touch pen 18 Press Tab to make Name turn red then press down direction key or click by mouse or keyboard user will see tables as below the left direction key is backspace key Ba a aa ia aaa aa a aa aa a paman aan a n aa a a lhe lhe l a S d f n j k l return C V b n A SAVE FILE After typing in a file name by keyboard or mouse click by keyboard or mouse press Enter on keyboard to finish typing Press Enter again or click OK to save file See below Notes When type characters by keyboard must make sure the previous one is black Or press Esc Or click return to quit Save As sro D Name salt CANCEL A SAVE FILE When the measurement is complete open the upper pedestal and wipe the sample from both the upper and lower pedestals using a soft laboratory wipe Simple wiping prevents sample carryover in the pedestals 4 Installation Insert the output end of power supply adapter into power connector in the back of instrument and connect the other end with network voltage AC100 AC240V Connect mouse to USB port to make operation more active 5 Software operation 5 1 Main interface Main interface composed by Set Acid Protein History Help click left button on mouse to enter interface you need or use up and Cow o then pices enter to choose eee Protein Hist
6. 06 0 00 012 009 130 009 0 00 O12 009 1 37 3 i bs ie BSA Olg O Lysozyme Protein Measure Ready 254 Touch Calibrate touch screen Enter into Touch interface Click OK or press Enter on keyboard to start calibrate click CANCEL or Esc on keyboard to quit system Set Are you sure Calibrate the touch screen Calibrate Touch Screen Notes After calibration system will prompt to restart Click ok to restart equipment Parameter factory Parameter Time time setting Click left mouse button or keys to enter into Time interface as follow Set Time Press Tab to choose setup type then press left and right direction keys to increase or decrease Example When setting up Hour press Tab only when HOUR turn red can increase or decrease the valve Click OK or press Enter to finish 5 3 Nucleic Acids measurement 5 3 1 Choose Acid on main interface enter into interface of Nucleic Acids measurement Dsdna O Ssdna O Rna A ank Acid Measure Program 2 04 5 3 2 Choose sample type Dsdna Ssdna Rna default setting is Dsdna Choose sample type frame by key Tab and choose one type by direction keys as below click spot behind type by click mouse or touch pen Dsdna O Ssdna O Rna a ank Acid Measure Read 2x0 5 4 2 Choose sample type 1Abs BSA IgG Lysozyme default setting
7. Micro Volumn Spectrophotometer For Nucleic Acid and Protein Model No YSA 301 User Manual CONTENTS Giapten 1 MMO GUCHON ssaesids teaceeewcedaenss need di teen 1 1 Full set of Equipment ccccecceeeeeeeeeeeeeeeeeas 2 Chapter 2 SpeciiCation szacie rn eee ieee cli 2 1 The Normal Operation Condition ccccceceeesseeeeeeees 3 2 2 The basic parameters and performance 0668 3 Chapter 3 Basic Operation 0 0 cccccsessseeeeeesesssssssseseeeeeeee 4 31 SIFUCIULE CESCHIDNOM eni nara sdenens 4 3 2 Sample size requireMeNntS 0 ccceee eee ee eee eeeeeeens 5 3 3 Basic use for pedestal c cece cece cece iiaea 5 Chapter 4 Software Setup ccceeec cece eee e ee eeeeeeeeeeeeaes 5 Chapter 5 Software operation cccccecc cece eeeeeneeeaeensees 6 91 me mammoa O s enn a a EAEAN 7 5 2 System Set interface ccc ccc a e 7 5 3 Nucleic Acids measurement eeeeee sneer eens 9 5 4 Protein A280 measurement c cceeeeeee eens 13 5 5 Ghieck AISO FECOldS renine a 18 Chapter 6 Repair and Maintain c ccc ceceeeeeeeeeeeeees 22 Chapter 7 Failure analysis and handling cccccccccsseeeeeeees 22 6 Repair and Maintain A A This product is an indoor Instrument Power off when you finish your work Pull off the connector plug when there s long time no use of the Instrument and cover it with a cloth or
8. into the down position Click Blank button to make a blank At this moment the button Blank is gray when it recover to default color calibration is finished Wipe the sample from both the upper and lower pedestals using soft laboratory wipe 5 3 4 Load a 2ul sample solution onto the lower pedestal and low down the upper pedestal into the down position Click Measure Cor key Measure on the keyboard At this moment the button Blank is gray when it recover to default color the measurement result will display on the table Default concentration unit is ng ul 5 3 5 When the measurement is complete use a new dust free paper to wipe the pedestals In this way the users can do next measurement lf measure the same kinds of samples the users needn t to re blank It is advisable to do the blanking every 15min at least Remind When measure different concentration samples please measure them in order of from low to high concentration If find the measure result is higher for same concentration Please re blank it 5 3 6 Users need to print one result press Tab to choose the table in a red frame then choose one result by keyboard press Enter the spot behind the result will brighten and press Print to start print If user want to print more than one result just need choose your target ones spots should be brightened 10 11 When users operate by mouse or touch pen Scro
9. is 1Abs Choose sample type frame by key Tab and choose one type by direction keys as below click spot behind type by click mouse or touch pen OiAbs OBSA OlgG Lysozyme ss Protein Measure Program Q 34 5 4 3 Create a blanking by using suitable solution Blank solution is normally solvent to dissolve the targeting molecular and it needs to be the same with the samples in PH and ionic concentration Load a 2ul blank sample onto the lower pedestal and lower the upper pedestal into the down position Click Blank button to make a blank At this moment the button Blank is gray when it recover to default color calibration is finished Wipe the sample from both the upper and lower pedestals using soft laboratory wipe 5 4 4 Load a 2ul sample solution onto the lower pedestal and low down the upper pedestal into the down position Click Measure Cor key Measure on the keyboard At this moment the button Blank is gray when it recover to default color the measurement result will display on the table 5 4 5 When the measurement is complete use a new dust free paper to wipe the pedestals In this way the users can do next measurement If measure the same kinds of samples the users needn t to re blank It is advisable to do the blanking every 15min at least 5 4 6 Users need to print one result press Tab to choose the table in a red frame then choose one result by keyboard press
10. ll through the table using the slider bar on the right of the window click the spot behind results to make spot brightened and click Print to start print F eT yi oos 07 Dsdna O Ssdna O Rna Is os 3 A Acid Measure Ready 5 3 7 When user want to skim measurement data press Tab to choose table in a red frame as above Skim tables by press up and down direction key also can Scroll through the table using the slider bar on the right of the window Sample type indicated on the table is the one chosen 5 3 8 Save data When need save Press Tab to make Save turn gray as below Dsdna O Ssdna O Rna Measure G Acid Measure Ready 251 Press Enter or click Save by mouse or touch pen enter into save interface as below Save As SaveTo Flash Press Tab to make Save To turn red press down direction key to choose save position or click Notes when spot behind the SD is gray means SD card has not b ctive or without SD card This moment user has to choose FLASH as below ne 3 CANCEL oe SAVE FILE Or choose position by clicking button Y using mouse or by touch pen Press Tab to make Name turn red then press down direction key or click by mouse or keyboard user will see tables as below the left direction key is backspace Key r E E E EE Ca 12
11. ory Welcom to NANO 200 is 3i Set System set Acid Acid detect Protein protein detect History check history records Help Documents for help Click this icon system will return to main interface 5 2 Set interface Enter into Set interface by mouse or by touch screen press kiad Esc or click close to quit System Set Touch Parameter System Set Press Enter or click Save by mouse or touch pen enter into save interface as below aa one ee oT save B m 5 Press Tab to make Save To turn red press down direction key to choose save position or click v Notes when spot behind the SD is gray means SD card has not been active or without SD card This moment user has to choose FLASH as below a E E E E E a a S E a F J T yA ees r x f 1 i CANCEL SAVE FILE Or choose position by clicking button using mouse or by touch pen 16 15 ig 1Abs OE BSA G O Lysozyme Protein Measure Ready 254 5 4 7 When user want to skim measurement data press Tab to make table in a red frame as above Skim tables by press up and down direction key also can Scroll through the table using the slider bar on the right of the window Sample type indicated on the table is the one chosen 5 4 8 Save data When need save Press Tab to make Save turn gray as below Wy le NO 250 230 Abs230 Abs260 Abs280 260 28
12. pen points brighten then click Print 5 5 3 User can connect YSA 301 to PC by USB this moment PC will identify YSA 301 as a U disk Data in YSA 301 can be copied but user can not operate YSA 301 when copy data After copy user have to power off and restart YSA 301 if want to continue measuring and 1 Introduction Thank you for purchasing our Products Micro Spectrophotometer YSA 301 This Manual for users contains function and operation of the Instrument In order to use the instrument properly please read this manual carefully before using the Instrument Keep it for later use when you meet with difficulties 1 1 Full set of equipment Micro Spectrophotometer 1 Unit Power Adaptor 1 Unit USB Connector Line 1PC Mouse 1PC Touch Pen 1PC Printing Paper 2 Rolls Manual 1PC Warranty Certificate 1PC Approved Certificate 1PC 2 Specification test txt 2 1 The Normal Operation Condition zee ot eee ee Ambient temperature 5 C 35 C iain say SE peste E The relative humidity lt 70 g txt prea 1 tet Power Supply DC24V 4A tests xt 2 2 The basic parameters and performance Medes YSAQON o test4 txt festa Gct 4 FLASH_DATA Acid Detector Type Ultraviolet silicon photocell Press key Enter to open this file Or click fel Y mouse or by Wavelength Range 230nm 260nm 280nm touch pen on screen to open Wavelength Range If users want to delete one file choose the one and press key Tab
Download Pdf Manuals
Related Search