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307 Piston Pump User's Guide

1. C Mbar 7 Carbon tetrachloride 20 103 105 ERN 25 106 108 Thevaluesofisothermal compressibility given 30 112 113 below can be used for the Compressibility 40 120 122 value in the pump setup menu These values Ethanol 20 110 112 are given under atmospheric pressure X 25 114 116 and are expressed in Mbar 7 30 118 119 40 126 127 The following table refers to Handbook of Acetone 20 123 127 Chemistry and Physics CRC Press 60th Ed 25 124 1979 30 133 40 144 156 Liquid Temperature Compressibility Methanol 20 121 123 C Mbar 7 25 125 127 Water 20 46 30 129 130 25 46 40 138 30 1 n Heptane 20 140 145 30 24 25 142 149 Benzene 20 94 95 30 150 155 25 96 97 40 160 2 oou n Hexane 20 150 165 25 161 171 Chloroform 20 97 101 30 165 180 25 97 40 183 M 1 Diethyl ether 20 184 187 25 195 200 Methylene chloride 25 97 30 208 209 2 OTHERDATA For other currently used liquids at ambient Liquid Liquid Compressibility temperatures 20 25 C the following data is Mbar 7 given This data is a result of experiments done Acetonitrile 99 using the 307 pump the figures are not pre Tetrahydrofuran 93 sented as physical constants of scientific value Water methanol 10 90 v v 117 If no data is available in this Appendix for the m liquid you use and if you wish an accuracy error 50 50 52 within the specifications you can experimen 60 40 46 tally determine
2. One complete method is stored in a file In order to read edit write a method you have to go to the file To go to a file press Menu press File This brings you to the Select file menu Key in the number of a file for example 1 and press Enter There are five soft key options available DIRECTORY Press this key to go to each stored file e g File 1 File3 etc This displaysallofthe files where method programs are stored If no programs are stored as in the case of anew pump Select file will be displayed Key in the file number that you want to use and press Enter Corv Press this key to make a copy of a complete file This is useful if you want to make a small modification to an existing program and keep a copy of the original program After pressing copy key in the file number where the copy will be stored and press Enter The software indicates if the file where you want to store the copy is empty New or if there is a program already stored there Exists You have the choice of completing the copy procedure Yes or ending the procedure without making a copy No The copy of the program can then be modified without destroying the original DELETE Press this key to delete a complete file The deleted file is the file currently shown on the upper line of the display After pressing Del Delete file xx is displayed This is a safeguard against accidental erasure There are two options Yes del
3. THEIN t 11NPUT This input only operates in the Program mode It is fully programmable It can be timed event Input Result used to make the program waituntilapiece wait input 1 Closed Open Wait IN 1 is closed of equipment is ready An example for this input is when the pumping system is wai ting for a signal from a sample injector See programming the inputs in chapter 4 for more information Wait input 1 Closed Closed Continue with program Wait input 1 Open Open Continue with program Wait input 1 Open Closed Wait IN 1 is open THEIN 2 INPUT This input is activated when closed Activating this Mode input while Program mode is selected causes File 13 to run If nothing is programmed in File 13 or if File 13 is already running this input will be ignored This input Program Closed Start File 13 Input Result Program Open No effect is only activated when the input changes from open to closed or from closed to open This input can be used to start a special program if an external signal is received for example a warning signal from a temperature measuring system 6 2 OUTPUT CONTROLS The 307 pump has four output relays Outputs 1 2 and 3 consist of two terminals These terminals can be connected together contacts closed or not connected together contacts open Output 4 consists of a common a normally closed and a normally open contact
4. Leaks from the hole at the bottom of the pump head Defective piston seal Replace piston seal Refer to User s Guide for the pump head Low flow rate Leaks Plugged inlet filter Defective check valve Pump head not mounted properly Check for leaks Clean or replace the inlet filter Refer to User s Guide for the pump head Clean or replace the check valve Refer to User s Guide for the pump head Check that the pump head is properly mounted Air bubbles appear in both inlet and outlet tubing Loose connection of inlet tubing Worn flange of inlet tubing Inlet filter partly clogged Refill time is too short for the solvent Tighten the connection but do not overtighten Replace the inlet tubing Clean or replace the inlet filter Increase the refill time Air bubbles appear only in outlet tubing Loose connection of outlet tubing Tighten the connection but do not overtighten Poor dampening effect Defective membrane in the manometric module Return to Gilson Eluent from the column is coloured blue Manometric module membrane is broken Return to Gilson 33 34 APPENDIX Accessory PARTS LIST 1 STANDARD ACCESSORY PACKAGE Ref Qty Description E45388 1 Pump head clamp 506341 1 Terminal block connector 14 pin 610101 1 Double ended wrench 1 4 5 16 502046 4 Fuses 2 0 Amp type T slow blow for 100
5. ofthe outputs are electrically isolated from each other and from ground These contacts can be used to control other equipment e g to turn on or off another piece of equipment See programming the outputs in chapter 4 for more information 12 OPERATION 1 SWITCHING ON THE PUMP After switching on the Model 307 a display appears for one second This indicates the software version currently V2 1 After this step one of the Ready to Run Screens is displayed 14 HYDRAULIC PRIMING Pump Model 307 V x xx Check that the solvent bottle is filled with HPLC grade degassed solvent or buffer Immerse the inlet tubing filter into the solvent reservoir Make sure that all of the hydraulic connections are properly made Do not run the pump head dry severe pump head damage can result Use the syringe supplied with the pump head to prime the pump Attach the syringe to the luer fitting of the low pressure prime valve See Fig 9 e Draw liquid into the syringe with the low pressure prime valve in the SYRINGE LOAD position Turn the valve to the SYRINGE INJECT position Press the PRIME key on the front panel of the 307 the pump will start running at its maximum speed Depress the syringe until the pump inlet is clear of bubbles and some liquid has passed through the pump outlet Turn the valve to the RUN position Remove the syringe from the low pressure prime valve When no bubbles can be seen at the
6. the display returns to the Ready to Run screen 29 30 8 PROGRAMMING EXAMPLE EXAMPLE Inthe example given in List 1 the flow rate will start at 1 ml min and increase to 4 ml min after 2 minutes An output will close at 0 00 minutes to start the integrator The method will only operate once and will not link to another file when it is finished Enter this program follow ing the steps given in Program list 1 Note that it is necessary to program a flow rate of 0 ml min at the end of the program to stop the flow the flow does not automatically stop when the program is finished It will continue with the last programmed flow rate To stop the flow at 4 00 minutes you must program a flow rate of 4ml minute at 3 99 minutes and 0 00 ml min at 4 00 minutes Note on programming sheet An example program is given below Before running this example program ensure that the hydraulic circuit is properly connected 44 Flowrate mL min 2 1 0 0 1 2 3 4 Time in minutes Figure 14 Appendix G contains a programming sheet which can be copied An example of how to use the programming sheet is also given The sequence you should follow is e Fill in the File Number and name Fill in the SETUP parameters for your system Draw the flow rate gradients on the graphs provided remembering to mark the axes Fillin the table for Input Outpu
7. 120 V 5 x 20 mm 500006 1 Power cord for 100 115 V 502145 4 Fuses 1 0 Amp type T slow blow for 220 240 V 5 x 20 mm 500005 1 Power cord for 220 240 V 801171 1 Model 307 User s Guide 410085 3 Nut 3161 1 16 hdc 1 4 28 TPI 410086 3 Ferrule 3161 1 16 hdc 430019 1 Tubing 316L 200 x 1 6 x 0 5 mm EG Only one power cord and one set of fuses are supplied The standard accessory package that you receive will contain suitable parts for your voltage 2 SPECIFIC ADDITIONAL ACCESSORIES Ref Description 500933 GSIOC cable 500944 Four wire electrical cable 1 7 m length for 1 0 connections Several units may be required according to the system configuration 267020 Mast clamp 267022 Mast aluminum 450 x 16 mm hexagonal MODEL 307 PISTON PUMP APPENDIX 5510 CONTROL This section explains how to control the 307 from a computer using Gilson HPLC system controller software 1 GSIOC FEATURES GSIOC stands for Gilson Serial Input Output Channel This communications channel links all of theGilsonmodulesinasystem together Thesystem controller controls all ofthe modulesina system by sending GSIOC commands to the slave modules for example pumps or detectors Each device connected to the GSIOC channel is distinguished by a GSIOC identity number between 0 and 63 The GSIOC identity number is set by switches inside each module or by the modules software The controllercommunic
8. Duiuuny uns 0 Apeay XX all 219 90 Xxx sdool jo Jaquinyy 611215 xx 01 xx Adog pesn 152 0 suarga sAemje Ae IND 941 S A dnjas daay 0 912 9 92 5 pebueuo sey 40195 ayoN yno 1X9N PdIN 291055810 19ju 40 19599 10 Ald PAN XXX 29715 yno Md K N XXX iqissardwog yno 1X9N UND poN ei dwng wa nuaw 123196 39 40 APPENDIX 307 PROGRAMMING SHEET File Number Method Name SETUP PARAMETERS FLOW Pressure High limit Low limit Units Loops Link file Solvent Refill Comp Headsize ml min 1 0 OPERATIONS Time min Time Contact State Function PROGRAM STEPS Step No Time Event Operation Step No Time Event Operation 1 14 2 15 3 16 4 17 5 18 6 19 7 20 8 21 9 22 10 23 11 24 12 25 13 APPENDIX G File Number Method Name SETUP PARAMETERS Pressure Highlimit 600 Lowlimit AO Units Bar Loops Link None Headsize Function Om GA O Stach intearetoCc d MODEL 307 PISTON PUMP 307
9. EN 50082 1 for immunity Gilson 307 Piston Pumps conform to EEC Directive 73 23 EEC for safety Standards EN 61010 1 UL 3101 1 and CAN CSA C222 N 1010 1 Each Piston Pump is subjected to a Dielectric Test 1500 V between live neutral and earth and an Earth Bonding Impedance Test lt 0 1 Q 43 APPENDIX H MODEL 307 PISTON PUMP TECHNICAL DATA ENVIRONMENT Dimensions W x D x H Temperature range Humidity Altitude Voltage range Electrical power Weight Pollution Installation 330 x 330 x 150 mm 4 40 C Up to 80 Up to 2000 m 100 120 V 220 240 V 50 60 Hz 120 VA 11 5 kg with pump head Pollution degree 2 Category 43 44 INDEX MODEL 307 PISTON PUMP Column cleaning conditioning Compressibility Dispense mode Electrical connections control power Error files layout operation programming Flow mode Fuse rating replacement GSIOC 305 commands identity number Installation control connections electrical hydraulic input outputconnections mechanical positioning the modules Mastinstallation Outputs initial conditions programming Pressure high limit low limit setting to zero Priming Program mode 29 29 15 20 ND 21 26 26 19 35 NO 17 25 16 16 18 12 21 Programming method Pump head installation maintenance size Refilltime Technical Data Timed event flow rate operate an outpu
10. If thereis a high pressure error the pump will stop and the message High pressure limit will flash on the screen The pump will start again when the pressure drops below the limit This cycle will continue indefinitely If it is programmed to be on the alarm will sound If there is a low pressure error the pump will stop and the message Low pressure limit will flash on the screen The pump will stay in this condition until the Stop key is pressed If it is programmed to be on the alarm will sound The maximum limits depend on the Refill time and Compressibility If the values are not compatible with the flow rate entered the message Invalid settings will flash on the screen after the Run key is pressed In this case you must lower the refill time or the flow rate The Flow mode can be simulated in the Program mode with the advantage of having safety error files and being able to program timed events 19 20 4 4 USING THE DISPENSE MODE 41 RUNNING THE PUMP IN DISPENSE MODE In this mode the pump delivers a specified volume beginning when the Run key or start input is activated and finishing when the specified volume of liquid is delivered The parameters are dispense volume dispense flow rate or time of dispense To go to the Dispense mode pressMenu press Disp This brings you to the Dispense mode Ready to Run Screen Two parameters are displayed on the top line the d
11. Open Start program Stop program EG In Dispense mode the Start input can be activated with a pulse MODEL 307 PISTON PUMP INSTALLATION 3 TurPAUsE RESUMEINPUT Mode Input Result The pause resume input is used to pause and Flow Closed Pause flow restart the 307 pump using an external contact Open Resume flow after Pause This input is only activated when the input Dispense Closed Pause flow changes from open to closed or from closed to Open Resume flow after Pause open The operation for each mode is given in Program Closed Pause program the table opposite Open Restart program In the Program mode this input can be configured to obtain a pause with or with Pause Prog is w flow out flow This choice is offered inside the Next Prev Change Quit software branch I O within the screen The default value is with w flow press change to obtain a pause without w o flow During a pause with flow the flow will continue with the flow rate and composition which existed when the pause input was activated The program will continue from the same point in the program when the resume contact is opened The pause without input is particular interest when the 307 is used as a metering pump to feed a reagent in high pressure reactors In this application the 307 can wait for correct values of external parameters temperature pressure composition before adding more reagent
12. PROGRAMMING SHEET EXAMPLE FLOW x P f mL min 4 1 44 Time 5 41 42 APPENDIX H TECHNICAL DATA Programmable reciprocating pump with single piston interchangeable head constant stroke and fast refill motion internal pulse dampener and pressure feedback WORKING RANGE Flow rate 0 010 5 ml min programmable down to 0 5 ul min Pressure 0 1 60 MPa Temperature 0 40 C for the module Pump Any Gilson model working in the above range specifically the 5SC for standard applications and the 10 WSC for salt concentrated solutions gt 0 1M LIQUID CONTACT MATERIALS 316L stainless steel sapphire ruby PCTFE PTFE HDPE PULSEDAMPENING Pulsation less than 1 with water at 1 ml min and pressure higher than 8 MPa Dampener volume 0 6 ml at atmospheric pressure and 1 6 ml at 60 MPa FLOW RATE PRECISION AND ACCURACY AT 20 C OVER FULL WORKING RANGE e Coefficient of variation 0 1 0 6 with aqueous solutions or hydro organic polar solvent mixtures and 0 3 1 with hydrocarbons or chlorinated volatile solvents Maximum accuracy error 1 with water over the full flow rate and pressure ranges OPERATING MODES Constant flow rate Flow constant volume Dispense and time based sequence Program PROGRAM MODE AND SYSTEM CONTROL Time adjustable from 10 to 10 mi
13. USING THIS MANUAL The 307 Piston Pump is a precision instrument which is simple and easy to use To gain the maximum from the instrument you should Read the description of the instrument in chapter 2 nstall the instrument as shown in chapter 3 Follow the operating instructions given in chapter 4 2 UNPACKING The 307 Piston Pump is packed single carton Upon receipt of your instrument carefully unpack the unit and inspect it for possible damage Check the contents of the carton against the parts list to verify that all parts are included and undamaged The parts list is given in Appendix A Do this now even if the unit will not be used immediately Report any damage to the responsible carrier immediately Read the description in chapter 2 to become familiar with the instrument its different parts and their names 3 WARRANTY If the instrument does not appear to function correctly first verify the electrical connections are correct and that the instrument is switched ON Contact your Gilson agent for technical advice or an eventual service visit Any service required will be given within the warranty conditions assured by your Gilson agent 4 CUSTOMER SERVICE Gilson and its worlwide network of authorized representatives provide you with four types of assistance sales technical applications and service The service personnel of Gilson s representatives are able to serve you more efficiently if you provide the f
14. document 2 0 Amp type T slow blow for use where the power supply is between 100 V and 120 V 1 0 Amp type T slow blow for use where the power supply is between 220 V and 240 V Adequate protection such as ventilation must be provided if dangerous liquids are used in the analytical work If incidental spillage occurs carefully clean up the spillage taking into account the nature of the spilled liquid including all required safety measures This instrument must not be sterilized using an autoclave or any other mechanical device When you need to clean this instrument use one of the three following methods 1 Aclean dry cloth 2 Acloth dampened with water 3 A cloth dampened with soapy water Ifacloth dampened with soapy water is used to clean the instrument only domestic soap may be used No other form of detergent or chemical may be used Safety Symbols Symbol Explanation The following electronic and hazard symbols appear on the 307 Module Alternating current PROTECTIVE CONDUCTOR TERMINAL On Supply switch Off Supply switch Caution referto accompanying documents O Caution risk of electric shock MODEL 307 PISTON PUMP INTRODUCTION 1 The Gilson 307 Piston Pump is designed to produce accurate and reproducible flow rates in the range 0 025 to 5 ml min It can be programmed to give flow rate gradients and to synchronize with other instruments using the inputs and outputs 1
15. file If you link two or more files together you must ensure that they all have the same values for these parameters If you link files together which have different values the pump will not start After pressing Run the display will give you the message Note Setup has changed since file creation Pressing Ok gives you the choice of which setup parameters to keep by asking the question Keep original setup Yes No If you choose Yes the values which are stored in the first file in the sequence of files will be loaded into all of the files which are linked together If you choose No the values which are currently stored in the setup parameter memory will be loaded into all of the files which are linked together In the case of linking to an error file which has different setup parameters to the method file the parameters for the method file automatically replace the values which were written in the error file If a program has a link to a file which has nothing stored in it the program will not start when Run is pressed The message Link file does not exist is displayed and the alarm will sound After pressing Run the bottom line of the display changes to Roll Menu Pause Pressing Roll will display in turn current file loop number running time and program time current file loop number flow rate current file current loop total number of loops the time that the 307 is waiting on an input Pressing Roll once more brings you bac
16. outlet tubing press the STOP soft key to end the priming procedure 1 2 USING THE KEYPAD E Syringe Inject position Run position ds Syringe Load position Pump inlet Inlet filter 10 um Figure 9 The keypad consists of numeric keys dedicated keys such as Enter and Prime a two line 24 character display and five white soft keys The function of each part of the keypad is as follows MODEL 307 PISTON PUMP OPERATION 4 Numerickeys Used to enter numeric values PRIME Runs the pump at maximum speed HELP Displays help messages CANCEL Cancels a value before it is entered into the memory ENTER Enter a value into the memory The 24 character display is used to show flow rates and input output operations The bottom line of the display is used to present soft key options above the 5 white soft keys Pressing one of the white soft keys selects the option displayed directly above it The following soft key options will occur frequently and should be noted Quit Return to the Ready to Run screen Next Brings you to the next screen Prev Brings you to the previous screen Time is expressed in minutes and hundredths of a minute For example 2 50 minutes is 2 minutes and 30 seconds The words Key in mean enter a numerical value A flashing cursor underlines the current parameter being modified symbol is used to show the factory set value The symbol e is used to
17. show a key which must be pressed or a value which must be entered 2 SETTING UP THE PUMP The software for the 307 pump will be explained with the help of the flow chart in Figure 10 The flow chart has 4 software branches Pump This is used to enter data about the pump I O This is used to enter data about the overall system File This is used to write a program Mode This is used to select the mode of operation As can be seen from the flowchart the Ready to Run screens give the choice of Run or Menu Run will start the pump with the parameters that were last used Menu gives you the choice of going to one of the four menus With a new pump you must enter data about the pump and the solvent being used This is done using the Pump branch of the software You must also enter data about the overall pumping system for example high pressure limit low pressure limit etc This is done using the I O branch of the software This is only necessary for a new system or when the physical setup of the pumping system is changed An explanation of the Pump and I O menus follows 14 OPERATION Refill Time Liquid Compressibility Inlet Pressure Pump only Figure 10 READY TO RUN SCREENS SELECT BRANCH OF SOFTWARE End Pump 1 0 File or Mode High Pressure Limit Contacts Add or Delete an Event with without flow Add or Delete an Event Zero Manometric Module Menu or Pause Pau
18. specified volume is delivered After pressing Run the display changes to Roll Pause Press Pause to interrupt the dispense operation The display changes to End Continue Press End to terminate the delivery without dispensing any more liquid Press Continue to finish delivering the specified volume of liquid Press Roll to view the programmed dispense volume and the volume already dispensed Press Roll again to view the programmed time for the dispense and the time already elapsed Press Roll again to view the flow rate MODEL 307 PISTON PUMP OPERATION 4 4 2 4 3 MODIFYING THE VALUES During a run it is not possible to change the dispense volume or delivery rate The setup parameters can be reviewed and modified during a run Press Menu and follow the procedure as already described to modify the setup parameters OPERATION OF THE PRESSURE LIMITS If thereis a high pressure error the pump will stop and the message High pressure limit will flash on the screen The pump will start again when the pressure drops below the limit This cycle will continue indefinitely If it is programmed to be on the alarm will sound If there is a low pressure error the pump will stop and the message Low pressure limit will flash on the screen The pump will stay in this condition until the End key is pressed If it is programmed to be on the alarm will sound The Dispense mode can be simulated in the Program mode with the advantage of h
19. they are presented in the manual ELECTRICAL INSTALLATION The instrument requires two fuses to be installed The type of fuses required are 2 0 Amp type T slow blow for 100 120 V 1 0 Amp type T slow blow for 220 240 V For safety reasons Piston Pumps are delivered without fuses installed Fuses must be installed by the user upon delivery 11 INSERTING THE FUSES Voltage selection Fuse value V A Ensure that the power cord is not connected before starting 100 120 20 to install the fuses Follow the procedure below to install the 220 240 1 0 two fuses The voltage selector and fuse holder is located below the power socket See Figure 4 Pull the voltage selector out of the power receptacle This is done by gently levering the selector out using a small screwdriver Pull out the drawer as shown in Figure 4 Insert the first fuse into the clips Push the drawer back into position Pull out the drawer for the second fuse which is on the otherside ofthe voltage selector Insert the second fuseinto the clips The following fuse ratings are used depending on the volt TERT age selected The two fuses are included in the standard i did accessory package 12 SELECTING THE VOLTAGE The 307 Piston Pump can be set to operate at 100 120 volts or 220 240 volts The different voltages are selected depending on the orientation of the fuse holder Tosetthe voltage to 100 20 volts Insert the fuse holder wit
20. to control the system 17 18 OPERATION 4 ZEROPRESSUREREADING The Zero soft key is used to set the pressure reading to zero when there is zero pressure in the system This ensures accurate pressure readings when the pump is running Before pressing Zero make sure that the pump has stopped and the pressure has dropped to zero otherwise further pressure indications will be incorrect If the operation is successful the message Pressure reading is zero is displayed If the operation is not successful due to pressure in the system the message Not done check pressure is displayed You have now completed the Input Output parameter setup Press Quit to leave this section of the software This will bring you back to the Ready to Run Screens LEAVINGTHESETUP MENUS The two menus pump parameter setup and I O parameter setup are now completed The 307 pump has all of the information that it needs The next step is to operate the 307 pump After entering the data about the pumping system the pump is ready to run The 307 pump can operate in 3 different modes These modes are Flow The 307 pump provides a constant flow rate The pump starts when the Run key is pressed and stops when the Stop key is pressed Dispense The 307 dispenses a specified volume The pump starts when the Start key is pressed and stops when the specified volume has been dispensed Program The 307 controls a complete system In this mode the 307 p
21. 3 activating input 2 will have no effect and the method program will continue If there is a program stored in File 13 when input 2 is activated File 13 will start File 13 will start to run even if the system was not previously running If there is a link to another file at the end of the program the linked file will start to run POWER FAILURE ERROR FILE The power failure error file will only operate if there is a power failure while a method program is running The sequence of events after a power failure is as follows If there is no program stored in File 14 after the power is restored the program will go back to the start of the method file and wait for a start input If there is more than one file in the method i e one file is linked to another the program will go back to the beginning of the first file in the method If there is a program stored in file 14 after the power is restored the program in file 14 will be run If there is a link to another program at the end of file 14 the linked file will start to run If the alarm is on it will sound If there is a power failure in the Flow or Dispense modes it has the same effect as if the pump was turned off and turned on again The screen presented will be the Ready to Run Screen of the last used mode 27 4 7 RUNNING A METHOD PROGRAM Atthe end of programming the method and error files the software returns to a Ready to Run Screen The t
22. 307 Piston Pump L GILSON 4 SOLUTI IONS AT WORK FOR YOU 307 Piston Pump User s Guide LT801171J 2001 Gilson SAS rights reserved February 2001 CONTENTS MODEL 307 PISTON PUMP INTRODUCTION Using this manual Unpacking Warranty CustomerService 2 DESCRIPTION Frontview Keypad Rear view INSTALLATION Electrical installation Mechanical installation Positioning the modules Control connections Hydraulic connections Input Output connections 4 OPERATION Switching on the pump Setting up the pump Using the Flow mode Using the Dispense mode Using the Program mode Programming the error files Running a method program Programming example 5 MAINTENANCE 6 TROUBLESHOOTING Electrical problems Hydraulic problems 7 APPENDICES A Accessory parts list GSIOC control Solvent miscibility table Liquid compressibility values Flowrate accuracy principle 307 Programming chart 307 Programming sheet Technical Data TAHMINAN INDEX Q2 O CO ON Og NO NO NO O ON 12 13 19 20 21 26 28 32 33 33 33 SAFETY Please read this section carefully before installing and operating the equipment The instrument described in this User s Guide is a programmable piston pump that can give flow rate gradients and can synchronize with other instruments using rear panel input and output connections It should only be used in the laboratory or similar indoor en
23. INSTALLATION The mast is used to stabilise a system when several modules are stacked on top of each other It is used to hold the prime purge valve and a manual injection valve The mast clamp should be installed before positioning the 307 pump in a system The mastis added after allof the modules have been put in place Follow the procedure below to install the mast clamp Remove the side screw holding the module cover See Figure 6 Screw on the mast clamp Fixoneclamp onto each module in the system After all the modules have been positioned the stainless steel mast can be secured within the clamps Figure 6 MODEL 307 PISTON PUMP INSTALLATION 3 POSITIONING THE MODULES Before putting each module in position make sure that each module is ready i e that the fuses have been installed and that any mechanical installation is finished The physical positioning of each of the modules in your system will depend on your type of system A suggested layout is given below for a 307 pump and a Gilson 231 401 auto sampler Thisis an auto analytical system with 1 pump 1 detector and 1 auto sampler The 307 should be on top This makes it easy to read the display and to use the keypad Figure 7 4 CONTROL CONNECTIONS The 307 pump and the other modules in a Gilson system communicate using the Gilson Serial Input Output Channel GSIOC Each module in a system has a GSIOC connector on its rear
24. ROFORM CYCLOHEXANE CYCLOPENTANE CHLOROETHANE CHLOROMETHANE METHYLFORMAMIDE METHYL SULFOXIDE OXAN THYLACETATE THYL ALCOHOL ETHYLETHER EPTANE EXANE ETHYL ALCOHOL METHYLETHYL I PROPYL ALCOHOL DI PROPYLETHER TETRACHLOROETHANE TETRAHYDROFURAN TOLUENE TRICHLOROETHANE WATER m XYLENE s m m MISCIBLE Bl wwisciece DIMETHYLFORMAMIDE DIMETHYL SULFOXIDE lll DIOXAN METHYLETHYL KETONE DICHLOROMETHANE PENTANE C TETRACHLORIDE CHLOROFORM I PROPYL ALCOHOL DI PROPYLETHER TETRACHLOROETHANE TETRAHYDROFURAN TOLUENE CYCLOHEXANE DICHLOROETHANE DI ETHYLETHER METHYL ALCOHOL TRICHLOROETHANE WATER XYLENE HEPTANE ETHYL ALCOHOL HEXANE ACETIC ACID ACETONE ACETONITRILE BENZENE BUTYL ALCOHOL CYCLOPENTANE ETHYLACETATE MODEL 307 PISTON PUMP APPENDIX D LIQUID COMPRESSIBILITY V ALUES 1 BIBLIOGRAPHY DATA Liquid Temperature Compressibility
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26. They can also be opened and closed manually To change the state of an output key in the number of the output For example output 2 When you press Enter the display will show the presentstate of output2 i e openor closed Press the soft key Change to change the state of the output By using this procedure each of the 4 outputs can be manually set to be open or closed The outputs will remain in this state until a method program is run and a change in the outputs is programmed E Thisisamanual method for setting the outputs to be open or closed at the start ofa run Setting the outputs manually is useful to check that output 1 turns on the integrator for example However when repeating the same operation many times it is better to program the output operations as part ofa method program In this way the outputs will follow the same sequence each time the method program is run Refer to programming the outputs later in this chapter The default state is open Press Next to go to the next menu lt WrrB WrrHovT FLow This option runs in Program mode only It enables the system to be controlled from an external sensor For example a temperature sensor can be used to pause the system if the temperature is outside a desired range The program and flow can be paused together or the program can be paused whilst the flow rate continues External parameters such as temperature pressure or composition can be monitored
27. a value to reach this goal 80 20 40 To do so use a trial and error empirical method Select the initial value for Liquid Compressibility according to the following guide lines For an organic solvent take a value given for the same or similar chemical family Fora mixture including salt aqueous solutions take the value of the dominant solvent Pump your liquid under high pressure to obtain a significant error preferably use a gravimetric method if you know the density of the liquid Then with a few successive approximations adjust the Liquid Compressibility value by assuming a linear relationship between this parameter and the resulting error 37 38 APPENDIX E FLOWRATE ACCURACY PRINCIPLE To generate the selected flow rate with high accuracy maintained under high pressure and for a variety of liquids the 307 software adds two complementary corrections to the basic piston flow rate Defined from the piston stroke volume only the piston flow rate is theoretically accurate at atmospheric pressure only The objective flow rate F is considered as the sum of three components F F F F F the piston flow rate decreases when pressure increases F the compensation flow rate for the liquid compressibility increases with pressure and F the compensation flow rate for all other factors also increases with pressure The piston flow rate F is defined by F N V with number of piston cyc
28. ate 1 ml min Add Add the next timed event Flow Enter the flow rate 2 ENTER At 2 00 minutes 4 ENTER Flow rate 4 ml min End Finish writing method file Solvent consumption 5 ml Quit Go to Ready to Run screen Menu File 11 ENTER New Edit 1 ENTER None Flow O ENTER 0 Quit Low pressure error file Go to file 1 Loop No link to other files Enter the flow rate At 0 00 minutes Flow rate 0 ml min Finish writing error file Program List 3 Key to press Notes Menu File 12 ENTER New Edit 1 ENTER None Flow O ENTER 0 Quit High pressure error file Go to file 1 Loop No link to other files Enter the flow rate At 0 00 minutes Flow rate 0 ml min Finish writing error file 31 32 MAINTENANCE 5 The 307 pump has been designed to require a minimum level of care and maintenance In practice maintenance is limited to cleaning and replacing parts of the pump head Check valves and filters can be cleaned Piston seals check valves piston assemblies anti extrusion gaskets and return springs can be replaced A maintenance kit is available for each model of pump head For details about maintenance kits and procedures see the User s Guide for your pump head The use of equipment for continuous unattended operation is becoming more and more important For this reason Table I gives an indication of replacement periods of maintenanc
29. ates with oneslave deviceata time The hardwareand softwarerequirements to control a module from a computer using the GSIOC are as follows An IBM PC AT XT PS 2 computer or compatible Gilson interface module 506B or 605 A Gilson HPLC system controller software package RS232C cable GSIOC cable GSIOC slave GSIOC slave Computer Gilson RS232C GSIOC interface Slave Devices Figure 16 To get started you must Install the Gilson HPLC system controller software into the computer Connect the computer to the Gilson interface using the cable provided with the interface Connect the output from the interface to the Gilson module using a GSIOC cable Start the Gilson HPLC system controller software Consult the GSIOC technical manual and the interface manual for more details 2 GSIOC COMMANDS The GSIOC commands can be used to control Gilson modules directly from a computer or from a Gilson HPLC system controller software package The use of the GSIOC commands is completely detailed in the 307 technical manual 35 APPENDIX C SOLVENT MisciBiLITY TABLE 1 SOLVENT MISCIBILITY TABLE Solvents should be miscible that is they should mix with each other in all proportions This is important during elution but also during solvent changeover For your convenience a solvent miscibility table is provided ACETIC ACID ACETONE ACETONITRILE BENZENE BUTYL ALCOHOL C TETRACHLORIDE CHLO
30. aving the safety error files and being able to program timed events 5 USING THE PROGRAM MODE 5 1 5 2 OvERVIEW In this mode the 307 pump can create flow rate gradients open and close outputs and wait for inputs The 307 pump controls instruments such as auto samplers and fraction collectors using the input output contacts on the rear panel of the 307 The Program mode can also simulate the Flow and Dispense modes with the advantage of safety error files and the ability to program timed events Before creating a method program in the 307 pump it is necessary to understand how the method programs are stored in memory MEMORY LAYOUT There are 14 files numbered 1 to 14 Each file can store one program There are 10 files available for method programs 1 to 10 Files 11 to 14 are for error safety programs A file contains timed events A timed event is a flow rate the operation of an input or the operation of an output One file stores a maximum of 25 timed events which make up the method program The error files 11 to 14 do not contain pre stored programs You write each error program in exactly the same way as a method program This allows you to program the sequence of events that will happen when an error occurs If an error occurs during a run the method program stops and the error program starts See later in this chapter for the exact operation of the error files 21 22 OPERATION 4 5 3 FILE MANAGEMENT
31. close or pulse Pulse means that the output will change its current state for 0 6 of a second Example Screen 1 3 00 min Output 2 Screen 2 Open Close Pulse To program this example press the Add soft key press Out key in the time for the output to operate 3 00 and press Enter key in the output number 2 and press Enter 25 OPERATION 4 The display will change to Open Close or Pulse press the soft key below close READING WRITING EDITING TIMED EVENTS The three different types of timed event have been explained above At the end of program ming each event the menu gives you five options These options are explained below Next Prev Add Del End displays the next timed event in the sequence If there are no more events it displays End file displays the previous timed event in the sequence If there are no more previous events it displays Beginning of file brings you to the Choose an event type menu to allow you to add another event to the present program deletes one timed eventin the program Asa safeguard it asks you Delete this point Press Yes to delete the event or No to return to the original menu leaves the software for programming timed events The pump displays Solvent consumption in ml for one complete run of the method program The minimum value is lt 0 1ml and the maximum value is gt 999ml Press Quit to go to the Ready to Run Screen You have now finished creat
32. common solvents at atmospheric pressure are listed in Solvent X Appendix D For CO compressibility of 1150 Carbon Dioxide 1150 Mbar should be entered for a pressure of 6 MPa at Water 46 7 C The values for the most common solvents are Methanol 123 Acetonitrile 99 default value is 46 for water the value for the solvent being used with pump and press Enter Size This parameter is the size of the pump head Possible values are 5 10 25 50 100 and 200 It is possible to use any head size with the Gilson 307 pump However to ensure accuracy reproducibility and efficient pulse dampening the flow rate should not exceed 5ml min The default value is 5 Key in the value for the pump head size and press Enter 15 16 OPERATION 4 Press Info to display the operating time for the pump head This time can be reset to zero by pressing Reset This is used to remember how many hours the pump head has been working since the last reset It should be reset every time the pump head has routine maintenance or when a new pump head is installed Press Quit to return to the Ready to Run screen IN LETPRESSURE The inlet pressure PO is the pressure at the inlet of the pump head This allows the accurate pumping of liquefied gas It mustbesettothesame value as the pressure of the aspirated liquid thatis the saturating vapor pressure at the ambient temperat
33. d size type of liquid and pressure MODEL 307 PISTON PUMP 307 PROGRAMMING CHART APPENDIX xx xx XX XXX XX XXX ino log 544 000 UJU XXX XX XXX XX XXX 110 293 dog Buidwey ON puoj XX XXX PEM XX XXX XXX 50001 XX XX Xo Xxpocxx XX 1510 NOY NI XX Xxx je QUI 0900 yno nue og 154 0070 Xx xx sneg XX asuadsiq uno wp3 90 do9 ino M N ad xx ap 199 95 5099105 asned 1109 01 910W xxxx sneg XXX XX juo 1544 0070 XX xxx pasned juo9 591 0070 pasneg A Su8819s Duiuung sned nua joy sdy 0070 XX sned nua 08 1594 0070 XX XXX XX XXX sned nu W 08 15 0070 XX XXX XX XXX dog nuaw Xx Xxx 9181 1504 000 A
34. e parts according to the type of use intensive regular or occasional The data in Table I assumes that the pump is working at half ofits maximum flow rate and pressure The nature of the liquid and the pump head model have only a small influence on these figures The time between each maintenance operation can be viewed by using the Info soft key in the Pump menu TableI Indicationof replacement pars yse Intensive Regular Occasional periods of maintenance parts 168 h week 40 h week 10 h week a type Piston seal 2 3 months 6 9 months 1 year Set of check valves 3 6 months year 2 years Piston assembly 6 12 months 2 3 years 5 years Anti extrusion gasket 6 12 months 2 3 years 5 years Return spring 1 year 2 3 years 5 years TROUBLESHOOTING MODEL 307 PISTON PUMP 6 1 ELECTRICAL PROBLEMS Problem Possible cause Solution Pump does not operate and power indicator does not light Incorrect voltage setting Power cord unplugged Fuse blown Check for power See electrical installation in chapter 3 Invalid settings flashing Refill time is too long for the flow rate programmed Lower the Refill time or flow rate Pump does not stop at end of program Not programmed To stop the flow at the end of a method program you must program a flow rate of 0 ml min 2 HYDRAULIC PROBLEMS Problem Possible cause Solution
35. e stored in the first file in the sequence of files will be loaded into all of the files which are linked together If you choose No the values which are currently stored in the setup parameter memory will be loaded into all of the files which are linked together UG Inthecaseof linking toan error file which has different setup parameters to the method file the parameters for the method file automatically replace the values which were written in the error file This brings you to the Choose an event type screen 23 24 4 MENU CHOOSE AN EVENT TYPE There are three different types of timed events You choose one of the different events by pressing the soft key below it The three timed event types are e Flowrate Program the flow rate ml minute Wait Wait for an input Out Activate an output FLow In this menu you set the flow rate for example 2 ml min There are two pieces of data for this menu Time at which flow rate occurs Flow rate in ml min Example 0 00 min 0 00 ml min 5 00 min 3 0 ml min To program this example press the Add soft key press Flow key in the time for the first flow rate 0 00 and press Enter key in the value for the flow rate 0 and press Enter press Add to add a second timed event key in the time for the second flow rate 5 and press Enter key in the value for the flow rate 3 and press Enter WE There will bea linear gradient between any two
36. etes the file No brings you back to the original menu Eprr NEw If there is no program stored in the file number that is displayed on the top line the display will be New if there is a program stored in the file the display will be Edit Both of these options will bring you to the first step in writing editing a method program Quir Pressing Quit brings you back to the Ready to Run Screens Pressing the Edit New key brings you to the first menu in the programming sequence Number of loops You are now ready to write a method program MODEL 307 PISTON PUMP 4 5 4 PROGRAMMING METHOD A complete method program is written by programming flow rates and the operation of the inputs and outputs The method program run time starts at time 0 00 i e when the start key is pressed and ends atthe time of the last timed event For example if you program the last event at time 20 00 then the run time is 20 minutes You must program every event for your method starting at time 0 00 If you donot program a flow rate at 0 00 minutes the pump will assume the current flow rate For a pump which is stopped the software will assume a flow rate of 0 ml min If the pump is running it will assume the current flow rate for time 0 00 It will then operate on a gradient between this value and the first flow rate in your program MENU NUMBEROFLOOPS The number of loops is the number of times that the program
37. ftware and may change from menu to menu The present functions are displayed above each softkey 4 PRIME the pump runs at its maximum flowrate until you press the STOP softkey 5 HELP displays advice and instructions at any time with no effect on the operation of the pump 6 CANCEL cancels your last entry before it has been stored in the memory 7 ENTER confirms a selection or parameter value and stores t in the memory 8 Numeric keypad this is used to key in values during programming The parameter being modified is always underlined with a flashing cursor Figure 2 DESCRIPTION 3 REAR VIEW Figure3 shows a rear view of the 307 Piston Pump with the electrical connectors The function of each connector is as follows GSIOCFROM CONTROLLER INPUT OUTPUT CONTROLS Powerswitch Power receptacle Connectionto a computer Connector for the 307 inputs and outputs On offpower switch Voltage selector and fuse holder INPUT OUTPUT CONTROLS START no 1 9 3 oe rrr 1 Power switch 2 Power socket 3 Fuse holder and voltage selector 4 Input Output socket 5 GSIOC from controller socket 6 Manometric module Figure 3 Rear view fT L4 MODEL 307 PISTON PUMP INSTALLATION 3 1 This chapter describes how to install the 307 pump It is recommended that you follow the installation instructions in the order that
38. h the numbers 110 120 on the bottom facing the small white arrow Tosetthe voltage to 220 240 volts Insert the fuse holder with the numbers 220 240 on the bottom facing the small white arrow 1 For safety reasons do not connect the power cord until you have finished assembling the instrument INSTALLATION 2 24 PUMP HEAD INSTALLATION 23 MECHANICAL INSTALLATION This section explains how to install the pump head the mast clamp and mast The pump head and mast clamp for each pump should be installed before positioning the modules The pump head is shipped in a hard case to protect it during transit Unpack the pump head from its case and check that all of the parts are included Follow the procedure below to install the pump head Insert the pump head into the front aperture of the pump See Figure 5 The notch at the bottom of the pump head body mustbe fitted on to the matching pin on the pump just below the aperture This notch ensures that the inlet port is on the bottom and the outlet port is on the top Holding the pump head in place with one hand set the clamp diagonally over the head Turn the clamp clockwise into position in the slots on both sides of the pump head Tighten the thumb screw until the clamp holds the pump head securely Make sure that the clamp ends are secured in their slots on both sides Figure 5 See the pump head User s Guide for more information MAST
39. he operator presses the Pause soft key followed by End 26 MODEL 307 PISTON PUMP 4 6 2 6 3 If the low pressure error file contains a program the method program will link to this file and will run it After file 11 is finished if there is a link to another file this new file will start to run If there is another low pressure error during file 11 the pump will stop and cannot resume until the operator presses the Pause soft key followed by End HIGH PRESSURE ERROR FILE If the pressure goes above the high pressure limit the sequence of events is as follows If the high pressure error file 12 contains no program the pump stops at the instant the pressure rises above the limit If the pressure drops below the limit again the pump will restart This cycle will continue indefinitely If the high pressure error file contains a program the pump stops and waits for the pressure to drop below the high pressure limit contained in File 12 When the pressure is below this limit file 12 will begin to run After file 12 is finished if there is a link to another file this new file willstart to run If there is another high pressure error during file 12 the pump will stop until the pressure drops below the limit and will then restart This cycle will continue indefinitely INPUT 2 ERROR FILE 6 4 When input 2 is activated it causes the following sequence of events If there is no program in file 1
40. ing a program method file Before running a method program you should program each of the four safety error files 6 PROGRAMMING THE ERROR FILES The error safety files are used to control the File Name Function pumping system if there is an error If an error occurs the method program is stopped and the 11 Low File runs after a low pressure error 12 High File runs after a high pressure error safety error Program is started The number and 13 Input File runs if input 2 is activated function of each file is listed opposite 14 Power File runs after a power failure These programs are created in exactly the same way as a method program To program the low pressure safety error file select file 11 and enter the sequence of flow rates and input output operations that you require when there is a low pressure error Similarly enter a program for the high pressure error file Input 2 error file and the power failure error file The Input 2 contact be connected to an external safety device such as a temperature or pressure measurement system Anexample of simple programs for the high and low pressure error files are given in program examples at the end of this section 6 1 Low PRESSURE ERROR FILE If the pressure goes below the low pressure limit the sequence of events is as follows If the low pressure error file 11 contains no program the method program will stop and cannot resume until t
41. ispense volume and the dispense rate Key in the dispense volume ml and press Enter The flashing cursor moves from below the dispense volume to below the dispense rate Key in the rate at which the specified volume will be delivered ml min Press Enter The second parameter can be changed from flow rate to time by pressing the soft key directly below Time In this case you enter the time in which the liquid is to be dispensed The limits for each of the parameters are as follows Maximum dispense volume 100 X head size ml Maximum dispense rate 1 X head size ml min Maximum dispense time 9999 minutes Minimum dispense volume 0 0001 X head size ml Minimum dispense flow rate 0 0001 X head size ml min The maximum dispense rate depends on the Refill time and Compressibility If the values are not compatible with the dispense flow rate or volume entered the message Invalid settings will flash on the screen after the Run key is pressed In this case you must lower the refill time or the flow rate If the dispense flow rate or volume is not compatible with the head size the software will not accept the value and you must key in a new value If the head size is modified so as to be too small after the dispense volume and flow rate have been entered the Run soft key will not appear in the menu when you return to the dispense Ready to Run Screen Press tostart the delivery of the liquid The delivery will stop when the
42. k to the Run screen The Menu soft key has the same function as before Press Pause to freeze the program The pump will continue to run keeping a constant flow rate The pump will stay in this state until another key is pressed When Pause is pressed the screen changes to Stop End Cont Stop will cause the pump to stop The method file remains in the Pause state Endwillcausethetermination of the method file without stopping the pump The pump will continue with the flow rate that existed when it was paused The screen returns to the Program mode Ready to Run Screen Cont will cause the program to restart from the point where it was paused N B To terminate a program completely and stop the flow you must press Stop followed by End More brings you back to the previous screen If a method program has terminated and the pumping system is still running press Cond followed by Stop WES The input Start Stop has the same effect as the End soft key in the Program mode During a method run the setup parameters and the method program can be modified Modify a parameter in the same way that you program it If the flow rate is modified during a run the new value will take effect from the instant it is programmed If a flow rate is modified while the pump is running the new flow gradient will be between the next programmed point and the flow rate which existed at the instant the modification was made After a method program has finished
43. les per unit time and V piston stroke volume The compensation flow rate for the liquid compressibility F is calculated as a function of five variables E Fy V Vy P X whereV is the volume of the dead space inside the compression chamber P the operating pressure and X the compressibility of the liquid under the pressure P In the 307 software X is calculated using the simplified Tait equation X P d Coefficient varies only slightly with the nature of the liquid It is a constant included in the software Coefficient d is calculated from the Liquid Compressibility at atmospheric pressure X for P 0 entered by the user as a set up parameter Values of X for some common solvents are tabulated in Appendix D Operating pressure P is continuously transmitted to the pump by the Manometric Module pressure feedback The complementary compensation flow rate for all other factors F is defined as the difference It was measured and the experimental results were expressed using a simple function of operating pressure P F f a b P Coefficients a and b were determined for each pump head They are manufacturing constants attached to the parameter Head Size a second setup parameter entered by the user of these factors taken together allow the 307 to deliver a highly accurate flow rate independent of the pump hea
44. n with increment from 0 01 to 1 min depending on the range used Program points up to 25 including flow rate and external events Flow rate adjustable in ml min from 0 01 to 100 of the head maximum flow rate External events 4 outputs contact closures to activate auto sampler integrator fraction collector and detector autozero and 1 input to wait for injection Upto999 program loops and program linking Safety functions 4 dedicated programs for pressure outside of selected limits input of an emergency signal temperature threshold leak detection etc and power failure MEMORY capacity 10 user s programs and 4 safety programs DISPENSE MODE Volume adjustable in ml from 10 to 10 times the pump head size For a 5 SC head from 0 5 ul to 500 ml Dispense time or flow rate be selected Pump PARAMETERS Head number 5 or 10 liquid compressibility 0 to 2000 Mbar 7 refill time 125 to 1000 ms and inlet pressure 0 to 10 MPa USER INTERFACE 2 x 24 character LCD display Front panel keypad Built in help messages Information display for use and maintenance INTERFACE Gilson Serial Input Output Channel GSIOC slave connector on rear panel ELECTRICAL INTERFACE 4 inputs and 4 relay outputs on rear panel CONFORMITY TO NORMS From serial 168001 Gilson s 307 Piston Pumps conform to Directive 89 336 EEC for electromagnetic compatibility Standard EN 50081 1 for emission Standard
45. ollowing information The serial number and model number of the equipment involved Computer model if used available memory microprocessor and operating software version s in operation The installation procedure you used A concise list of the symptoms list of operating procedures and conditions you were using when the problem arose e A list of other devices connected to the system anda system diagram showing the connections A list of other electrical connections the room DESCRIPTION 2 This chapter describes the physical layout ofthe 307 pump It describes thefront panel ofthe pump and the position of electrical connectors on the rear panel 1 FRONT VIEW Figure 1 shows a front view of the 307 pump with a pump head mounted There is a keypad which consists of a display a numeric keypad and soft keys for programming the 307 The pump head is mounted on the right hand side 1 Digital display 2 Numeric keypad 3 Pump head 4 Pump head clamp 5 Pump head inlet 6 7 8 9 Pump head outlet Manometric module connections Side grip Side screws Figure 1 MODEL 307 PISTON PUMP DESCRIPTION 2 2 KEYPAD Figure 2 shows the keypad with the numeric keys the display and the soft keys 1 Power on indicator 2 Display two 24 character lines are used to display parameters commands and messages 3 Softkeys their functions are determined by the so
46. op left corner will indicate which Ready to Run screen you are in To go to the Program mode Ready to Run Screen pressMenu press Prog The pressure can be displayed in 3 units bar MPa or kpsi To change the units press the soft key directly below bar The units will change to MPa Press the soft key again to change the units to kpsi Press the soft key again to change the units back to bar The Ready to Run Screen displays the present file number the pressure and gives you 3 soft key options Cond Menu and Run You can go to any file by keying in a new number and pressing Enter The Cond soft key brings you to the part of the software which is used to condition the column You program a ramp time and a flow rate The initial conditions are the existing conditions for the pump i e for a pump which is not running 0 ml min The flow rate programmed should be the flow rate that is required at the start of the method program The flow rate is then ramped from 0 ml min to the initial flow rate for your method program press Cond Enter the ramping time and press Enter press Flow Enter the flow rate for the end of the ramp time Press Run to start the conditioning of the column The pump will ramp to the flow rate that is programmed in the time programmed The values for time flow rate can be changed during the conditioning At the end ofthe ramp time the flow rate will remain constant Note that y
47. ou can leave the conditioning of the column and create a program without interfering with the conditioning of the column After pressing Run the display changes to give you the choice of Roll Stop or Quit Pressing Roll displays in turn the time for the ramp and the flow rate This part of the software is also used at the end of a method program to clean the column The initial conditions will be the final flow rate which existed at the end of your method program You can ramp the flow rate down to 0 ml min to completely clean the column TheQuitsoft key brings you to the Select Menu Item menu This gives you access to the pump setup parameters the pump I O parameters and the method file Press this key to verify or change the value of any parameters in these sections The Runkey starts the program running When this key is pressed the display changes The figure in the top left hand corner is the file number The number in parentheses is the loop number If there is more than one loop in your program the present loop number will be displayed here The first time displayed is the actual running time The second time displayed is the total running time for the method program MODEL 307 PISTON PUMP 4 EX Onecomplete method program is stored ina file The values for the five parameters Refill Compressibility Pump Head Size High pressure limit and Low pressure limit for that programare also stored in the same
48. panel and is connected to a system controller using a GSIOC cable This is the case when a 307 pump is controlled by a computer with a Gilson HPLC software package Toconnectthe307 pump toasystem controller Connect the socket marked GSIOC FROM CONTROLLER on the rear panel of the 307 pump to the system controller using a GSIOC cable 5 HYDRAULIC CONNECTIONS The hydraulic connections for the 307 pump head are made using the tubing provided in the standard accessory package Connect the 307 pump head input with the inlet tubing assembly provided with the pump head The connections to the 307 pump head output should be made using stainless steel tubing The outlet of the pump head should be connected to one of the two ports of the internal dampener transducer The other portis then connected to the rest of the system 6 INPUT OUTPUT CONNECTIONS For coordination with surrounding equipment electrical contacts are used The Input Outputconnectorisa 14 pin terminal block connector Connections are madetothe inputs and outputs using the connector supplied in the standard accessory package Inputs Outputs Start Stop Out 1 Pause Resume Out 2 IN 1 Out 3 IN 2 Out 4 10 INSTALLATION The function and pin numbers for each input and output are as follows OPERATION OF THE INPUTS Pin Function 1 Ground To activate an input you must connect it to ground or 0 2 Start Stop Inp
49. programmed flow rates With the above example the flow rate will linearly increase from 0 0 ml min to 3 0 ml min in 5 00 minutes If youtry to enter a flow rate value which is too high for the pump head the entry is refused by the software If you do not program a flow rate at 0 00 minutes the software will assume the current flow rate for time 0 00 minutes ie for a 4 stopped pump a flow rate of 0 ml min There will then be a gradient between 0 ml Flowrate 37 min and the first programmed flow rate ml min With the example programmed the flow rate will be as shown in Figure 12 To stop the flow ofsolventattheend ofa run 0 you must program a flow rate of 0 ml min 2 Ge ch d B Otherwise the pump will continue to run Time in minutes with the last programmed flow rate even after the last timed event Figure 12 If the system is set up to do more than one sample the initial flow rate for the method can be maintained between method runs by programming this flow rate at the end of the program for example 2 ml min MODEL 307 PISTON PUMP 4 At the end of all the samples link to another file to completely stop the flow of liquid See Figure 13 Method Program Wait for signal from sampler war In this menu you can make the program wait until Input 1 is activated This is used to stop the program untilanother piece of equipment 1 i is ready fo
50. r example an auto sampler or a the next sample fraction collector There are two pieces of data for this menu Time at which the pump will wait for an input Last timed Waiting for an open contact or a closed contact id Example 2 00 min Wait 1 Closed Link to new file after last sample To program this example press the Add soft key Stop flow press Wait key in the time for the wait to begin 2 00 and press Enter Figure 13 pressclose The program waits at time 2 00 minutes until input 1 is closed If input 1 is already closed at time 2 00 mins the program will continue If input 1 is not closed the program will wait During the time that the program is waiting the display will show the total run time and the time that it has been waiting If a pump is waiting for an input pressing Cancel will simulate an input and the program will continue In this menu you program each of the outputs in the system to open or close The outputs are numbered 1 2 3 and 4 and are used to send signals to other equipment in the system for example an auto sampler or a chart recorder By having the output operations as part of the method program the same sequence of contacts is repeated every time the method program is run There are three pieces of data for this menu Time at which output is operated Output number Output opened closed or pulsed Each of the outputs can be made to open
51. se End System is running Flow Dispense or Program MODEL 307 PISTON PUMP 4 2 1 SETUP PUMP HARDWARE PUMP To go to the setup pump hardware menu pressMenu pressPump The sequence of parameters is 1 Refill time 2 Liquid Compressibility 3 Head size A valuefor each parameteris keyed in using the keypad and isstored in memory by pressing Enter Pressing Enter automatically brings you to the next menu If you do not want to change the value already stored in memory press Next maximum flowrate 100 The Refill time is the time required for the piston return stroke Normally it is set at the lowest value INVALID SETTING 125 ms If cavitation or degassing occurs then a higher value must be used The minimum value is 125 ms and the maximum value is 1000 ms ES Themaximumflowratedependson the refill time If the refill time is too long a message Invalid settings flashes when you run the program The refill time or flow rate must be 50 lowered Figure 11 showsa curve of refill times versus flow rate The default value is 125 ms Key in the refill time and press Enter PUMP COMPRESSIBILITY 10 This data is used to calculate the flow rate compensation for the compressibility of the solvent minimum value is 0 and the maximum value is Figure 11 2000 Mbar Compressibility values for the
52. t wait for an input Troubleshooting electrical problems hydraulicproblems Voltageselection 23 32 15 15 42 23 25 33 33
53. t operations Write the programming steps Finish one section before going on to the next i e program all of the flow rate time points before going on to the input output time points The 307 software will arrange all of the time points in the correct sequence After writing the method program it is neces sary to write the programs for the safety error files Simple programs are given in list two and three for the Low pressure error file and the High pressure error file In both programs the flow rate is programmed to be 0 ml min This is a simple example of an error file Enter these two error programs before running Example 1 After entering the two pressure error programs the system is ready to run Press Run The display will look like Figure 15 When the method program is finished the Ready to Run screen is displayed File number Number of loop Elapsed time Run time 1 1 0 55 2 00 min 80 bar Roll Menu Paus Pressure reading Roll Menu Pause Figure 15 Program List 1 MODEL 307 PISTON PUMP Program List 2 Key to press Notes Key to press Notes Menu File 1 ENTER Select file 1 New Edit Go to file 1 ENTER 1 Loop None No link at end of method Out Program the output 0 ENTER At 0 00 minutes 1 ENTER Output 1 Close Close output 1 Add Add the next timed event Flow Enter the flow rate O ENTER At 0 00 minutes 1 ENTER Flow r
54. ump can create gradients of flow rate open and close outputs to control other instruments and wait for signals from other instruments The operation of each mode is explained separately MODEL 307 PISTON PUMP 4 3 USING THE FLOW MODE 3 1 RUNNING THE PUMP IN FLOW MODE In this mode the pump provides a constant flow rate beginning when the Run key or start input is activated and stopping when the Stop key or stop input is activated To go to the Flow mode pressMenu pressMode pressFlow This brings you to the Flow mode Ready to Run Screen The flow rate can be set between 0 01 and 100 of the pump head size A flow rate value will not be accepted if it is larger than the pump head size If the selected flow rate is incompatible with therefill time or compressibility the message Invalid settings blinks after pressing Run In this case you must lower the refill time or the flow rate Key in the flow rate in ml min and press Enter The pump is now ready to run Pressing Run will start the pump Pressing Stop will stop the pump 3 2 MODIFYING PARAMETERS DURING A RUN During a run the flow rate can be modified at any time by keying in a new value During arun it is possible to review and change the setup parameters without stopping the pump Press Menuand follow the procedure as already described to modify the setup parameters 3 3 OPERATION OF THE PRESSURE LIMITS IN THE FLOW MODE
55. ure for liquefied gas delivered from a pressurized cylinder When using carbon dioxide at a temperature of 22 C the value of the inlet pressure should be defined as 6 MPa A table of inlet pressures is shown below Key in the desired inlet pres Ambient Temperature C 15 20 22 25 30 31 T Co To sure the default value is 0 MPa Pressure PO MPa 51 58 60 65 72 74 e Press ENTER 22 INPUT OUrPUT PARAMETER SETUP I O The I O menu is used to enter data about parameters associated with the complete system To go to the Input Output parameter setup pressMenu press I O The sequence of parameters is High pressure limit Low pressure limit Alarm GSIOC identification number Output contacts Pause Prog with or without flow Zero pressure reading ogg HIGH PRESSURE LIMIT If the pressure reading from the manometric module rises above this limit the pump will stop The sequence following a high pressure error is described later in this chapter The pressure can be displayed in three different units bar MPa or kpsi Change the units by pressing the soft key below the units display bar MPa or kpsi The maximum value is 600 bars The default value is 600 e Key in the pressure limit that applies to your system and press Enter LowPRESSURELIMIT If the pressure reading from the manometric module drops below this limit the pump will stop The sequence following a lo
56. ut 8 3 Pause Resume Input Volts This is usually done using a relay output with one 4 INZ T Input side connected to 0 Volts Pin 1 and the other side con 5 IN 2 Input nected to the input When the output is closed the input is 6 Out 3 Output connected to 0 Volts and is activated See Fig 8 Each of the 7 Out 3 Output four inputs are described below in detail 8 Out 2 Output 9 Out 2 Output 057 In the Program mode Stop does NOT stop the flow it only stops 10 Out 1 Output theprogram from continuing Theflow will continue with theflow 11 Out 1 Output rate which existed when the stop input was activated The program b 1 asa nn T ormally close will restart from the beginning when the contact is opened 14 Out 4 Normally open Inputs to Output contacts the 307 from the 307 r ra 307 1 0 Connector Start Stop Input Pause Resume Contacts from other equipment Wait for Input 1 Activate Input 2 Output 3 Output 2 Output 1 Output 4 L E E E LC E D LH E D EI Figure 8 THESTART STOPINPUT Mode Input Result The start stop input is used to start and stop the 307 Flow UN pump using external relay contact This input is closed Start Dispens only activated when the input changes from open to Open Stop Dispense closed or from closed to open The operation for each Program Closed mode is given in the table opposite
57. vironment by qualified personnel If the instrument is used in a manner not specified by Gilson the protection provided by the instrument may be impaired For safe and correct use of the instrument it is essential that both operating and service personnel follow generally accepted safety procedures as well as the safety instructions given in this User s Guide Cleaning installation dismantling maintenance adjustment and repair should only be performed by personnel trained in such work and who are aware of the possible dangers involved Voltages present inside the instrument are potentially dangerous If there is a problem with the instrument the power cable should be removed until qualified service personnel have repaired it This is to prevent anyone from inadvertently using the instrument thus causing possible harm to themselves or damage to the instrument itself The leakage current ofthis instrument is within the limits allowed by safety standards for laboratory equipment An efficient ground connection is imperative for the physical protection of the user Ensure that the ventilation fan on the Piston Pump operates and is not obstructed when the instrument is installed Power supply cord reference 500005 is for use in France and Germany Power supply cord reference 500006 is for use the USA and Canada For other countries contact your local Gilson representative You must only use the type of fuse described and specified in this
58. w pressure error is described later in this chapter MODEL 307 PISTON PUMP 4 minimum value is 0 default value is 0 Key in the value that applies to your system and press Enter ALARM The alarm is a buzzer which sounds every time there is an error or an invalid setting It can be programmed to be either On or Off This function only controls the operation of the buzzer it does not affect the operation of the pump when there is an error If the alarm is On the warning buzzer will sound every time there is an error An error can bea pressure limit or an invalid setting This parameter can be changed from On to Off and vice versa by pressing the soft key Change Select the option that you want and press Next to go to the next menu GSIOCUNITIDENTIFICATION NUMBER A Gilson system can be controlled from a computer using a GSIOC interface and GSIOC cables Each instrument in a system must have a unique identification number to distin guish it from other equipment connected to the GSIOC communications channel The GSIOC identification number in the 307 can be set between 0 and 63 The default value is 1 Key in the identity number for the pump and press ENTER OurPurxxis CLOSED There are four relay outputs in the 307 pump numbered 1 2 3 and 4 These outputs are used to control other instruments They can be programmed to open and close during a method run
59. will repeat itself before stopping The minimum value is 1 and the maximum value is 999 The default value is 1 Key in the number of loops and press Enter This brings you to the When finished use menu Menu WHENFINISHED USE At the end ofa program you can link the current file to any of the 14 files If you do not want to link to any other file press the soft key below None on the display If you link to the current file i e link File 3 to File 3 the program will continue to run until the Pause key is pressed If both looping and linking are programmed the software will complete the programmed number of loops then link to the new file The default is None Key in the number of the file you want to link to and press Enter or press None EG One complete method program is stored file The values for the five parameters Refill time Compressibility Pump Head Size High pressure limit and Low pressure limit for that program arealso stored in thesame file If you link two or more files together must ensure that they all have the same values for theseparameters If you link files together which have different values the pump will not start After pressing Run the display will give you the message Note Setup has changed since file creation Pressing gives you the choice of which setup parameters to keep by asking the question Keep original setup Yes No If you choose Yes the values which ar

307 Piston Pump User's Guide

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