845Z Injection Module User's Guide
Contents
1. Stator Stator Screws 3 Exploded view of motorized valve 4 2 Replacing the Rotor Seal and Stator Face Assembly Refer to the diagram when replacing the rotor seal and stator face assembly To replace the rotor seal and stator face assembly 1 Remove the stator screws with the 9 64 Allen head screwdriver provided 2 Remove the stator and stator face assembly from the stator ring 3 Remove the stator ring 4 Remove the rotor seal by pulling it off from its 3 pin mounting on the stator ring 5 Mount the new rotor seal with the grooves facing you The shaft assembly with three pins fits into the mating holes in the rotor seal only one way 6 Replace the stator ring so the two short pins on the ring enter the unthreaded mating holes in the body 7 Mount the new stator face assembly onto the stator Confirm that the pins on the assembly fit in the mating holes on the stator Maintenance 10 11 Replace the stator and stator face assembly on the valve so that the pin in the stator ring enters the mating hole in the stator Replace the hex screvvs in the stator Finger tighten each an equal amount until snug Tighten vvith the 9 64 Allen head screvvdriver Set the flovv rate on the HPLC pump Check for leaks by touching the underside of the valve If there is a leak you vvill need to tighten each hex screvv on the leaking valve equally in gradual increments 4 3 Ajquiassy 10 52. water Dead volume 202 uL Probe volume 111 uL Calibrated tubing volume 91 uL Rinse Probe at rinse station aspirate 10 000 uL from reservoir at 100 mL min then dispense 10 000 uL at 20 mL min Appendix Injections 1 2 3 4 Two system blanks Two injections of 70 30 methanol water blanks One injection of 55 000 ug mL ethyl paraben in 70 30 methanol water Three injections of 70 30 methanol water blanks Repeat steps 3 and 4 four more times Calibration curve parameters External calibration Linear curve fit Five calibration levels 1 2 75 ug mL ethyl paraben in 70 30 methanol water 2 5 5 ug mL ethyl paraben in 70 30 methanol vvater 3 16 5 ug mL ethyl paraben in 70 30 methanol vvater 4 275 ug mL ethyl paraben in 70 30 methanol vvater 5 55 0 ug mL ethyl paraben in 70 30 methanol vvater Injection protocol Prep total loop injection Initialize your system i e home syringe at rinse station and home XYZ arm before running any method BON ON SD 1 11 Set injection valve to load Aspirate 30 uL air gap at 1 0 mL min Move to sample location 2 mm from bottom Aspirate 5100 uL sample volume loop fill 100 uL of 55 000 ug mL ethyl paraben in 70 30 methanol water at 3 0 mL min Wait 0 02 minutes to equilibrate Set injection valve to inject Coordinate with start of data collection Move to inside rinse station Home syringe Rinse probe aspirate 10 000 uL from outside r
3. 14 Technical Specifications Please be aware of the following before operating the injection module Note Changes or modifications to this device not expressly approved by Gilson could void your factory authorized warranty This device has been tested and found to comply with the limits for a Class A digital device pursuant to Part 15 of the FCC commercial environment This device generates uses and can radiate radio frequency energy and if not installed and used in accordance with the instructions may cause harmful interference to radio communications Operation of this device in a residential area is likely to cause harmful interference in which case you will be required to correct the interference at your own expense Shielded cables must be used with this unit to ensure compliance with the Class A FCC limits Introduction Technical Specification Definition Available sample loops 5 uL 10 pL 20 pL 50 uL 100 uL 1000 uL 2000 pL 5000 uL and 10 000 uL A 20 uL loop was installed in the analytical system used to determine the specifications a 5000 uL loop was installed in the preparative system used to determine the specifications see Appendix D Available valves Rheodyne RV700 100 for flow rates up to 20 mL min Rheodyne RV700 112 for flow rates up to 150 mL min Contact control One contact closure input and one contact closure output Dead volume Analytical 40 uL from
4. Sieg 1 4 Replacement Parts and Accessories Appendix 2507252 2507256 2507414 251646 27067361 27067373 27067374 27067375 27067382 27067383 A 4 A Micro septum piercing probe constricted 45 bevel tip capacitive level sensing stainless steel Dimensions 220 x 1 5 x 1 1 mm ID tip dimensions 10 x 0 7 x 0 4 mm ID Requires probe holder guide kit part number 253641 Beveled tip probe stainless steel grooved septum piercing 221 x 1 5 x 0 4 mm ID Requires probe holder guide kit part number 253641 Non septum piercing probe constricted tip capacitive level sensing stainless steel Dimensions 220 x 1 3 x 0 8 mm ID tip dimensions 1 5 x 0 9 x 0 45 mm ID Requires probe holder guide kit part number 253642 Inert gas probe assembly for maintaining an inert atmosphere inside sealed vessel beveled tip stainless steel strain relief design Dimensions 274 x 1 8 x 0 8 mm ID Includes probe holder guide kit Non septum piercing probe bevel tip capacitive level sensing stainless steel Dimensions 220 5 x 1 5 x 1 1 mm ID Requires probe holder guide kit part number 253641 Non septum piercing probe constricted tip capacitive level sensing stainless steel Dimensions 221 x 1 5 x 1 1 mm ID tip dimensions 2 x 1 1 x 0 4 mm ID Requires probe holder guide kit part number 253641 Non septum piercing probe constricted bevel tip capacitive level sensing s
5. System Software version 3 2 or higher Valve switching speed RV700 100 Less than 266 mS RV700 112 Less than 200 m5 VVeight vvith installed valve RV700 100 Control Box and Valve Box 1 7 kg 3 7 165 RV700 112 Control Box and Valve Box 1 8 kg 4 0 lbs Working dimensions WxDxH Control Box installed on 215 control cabinet 15 2 x 13 7 x 7 1 cm 6 0 x 5 4 x 2 8 in Valve Box installed on the 215 Z arm 7 9 x 19 3 x 11 cm 3 1 x 7 6 x 4 3 in 1 6 Installation 845Z Injection Module on a 215 Liquid Handler Installing the Injection Module Installation 2 2 remove these four screws 215 Control Cabinet Cover Installing the Injection Module Your 845Z Injection Module is installed on the Z arm on the 215 Liquid Handler and is connected to the 845Z control unit which is installed on the 215 control cabinet Refer to the following instructions for installing the 845Z Injection Module on the 215 Liquid Handler Removing the Tubing Support Rod Warning Before installing the 845Z Injection Module it is necessary to remove the tubing support rod that is shipped installed on the 215 Liquid Handler If this is not done the liquid handler cannot move to its home position and you may damage the injection module 1 Remove the six Phillips screws securing the control cabinet cover to the control cabinet control tubing
6. applications e 19200 e 9600 e 4800 1 Baud Rate Appendix C To change the baud rate 1 Turn off then disconnect the 845Z control unit 2 Remove the two Phillips screws that secure the 845Z control unit s cover and then remove the cover 3 Locate JP1 in the far left center of the electronics board 5 1 1 T H 17 eeeeecece ee ee c l elC41 pi4 eo eo H 2 tied 5 l RIDRS ee eo 9 9 S 9 e 11 0666 2 ee e cs l l 960660 C35 e eis ss cs s s sc u e e e e e ee e 9 999 9 o ccoooooo 4 Reposition the fumper to connect the pins for the baud rate that you vvant to use 5 Replace the cover and the tvvo screvvs that secure it 6 Re connect the 845Z control unit C2 Specifications Methods D This appendix contains information on the methods used to obtain the specifications listed on page 1 5 For an analytical system refer to pages D 2 to D 6 for a preparative system refer to pages D 7 to D 11 D 1 Specifications Methods Appendix D 2 Analytical System 215 Liquid Handler 125 mm Z arm clamped at 125 mm Micro septum piercing probe part number 2507252 constricted 45 bevel
7. at the address Reset NV RAM and initialize to default value Returns error code 0 No error 1 Positioning error 2 GSIOC command to move valve is received when the valve is not homed 3 GSIOC command to move valve is received while the valve is in operation 4 A buffered GSIOC command was sent with an incorrect parameter 5 Problem with connection or cabling Homes the valve Returns the valve type that is installed 0 RV700 100 Analytical 1 RV700 112 Prep B 3 Spueululo2 2OIS GSIOC Commands Appendix Pxxxx B R I V I VI B VL B VVIXI B B 4 Sets the valve to an angular setting in tenths of degrees For example buffered command P3300 sets the valve to 330 degrees Reads and returns the LED display character Reads and returns the valve status L Load position I Inject position M Moving E Error Valve has not been homed Rotates valve to Inject position Rotates valve to Load position Writes to the LED display where WX Indicates GSIOC ownership of the display and writes X to the display VV Relinquishes GSIOC ownership to the unit for control of the display Baud Rate C As a default the baud rate is set to external indicating that the 845Z Injection Module is a slave device in a Gilson system and the baud rate is being clocked externally The following baud rate selections are available if the 845Z Injection Module is being controlled by non Gilson
8. injection module Push the terminal connector in as far as it will go It is designed to fit snugly into its receptacle 6 Connect the opposite ends of the wires to the other device Be sure to match ground connections 7 Label each cable to identify the purpose of the connection Installation Power Connections Use the supplied AC adapter to connect the power receptacle on the 845Z Injection Module control unit to an AC power source Refer to the rear panel diagram on page 2 4 Connect the 12 pin cable attached to the injection module to the control unit The connector pushes on and pulls off Don t twist The outlet from the control unit is located on the rear panel See rear panel diagram on page 2 4 2 9 suon uuo2 2911399 Bupieyy Making Plumbing Connections Installation Valve to probe tubing connection 2 10 Making Plumbing Connections Refer to the following pages to make the plumbing connetions The analytical instructions should be used with the 845Z Injection Module part number 251551 and 845Z plumbing package part number 250550152 The preparative instructions should be used with the 845Z Prep Injection Module part number 251552 and 845Z prep plumbing package part number 250550252 Sample Loop The 845Z Injection Module is shipped with the sample loop installed The loop runs between ports two and five of the valve Column and Pump Tubing Analytical Connect the 0 3 mm ID T
9. outlet right port on your syringe pump Connect the 0 8 mm ID calibrated Teflon tubing pre cut to 18 cm and supplied in the plumbing package from the top of the probe holder to port six of the injection valve 2 11 Operation 3 The 845Z Injection Module is controlled via a method running on a computer You create the method using UniPoint System Software ordered separately See Appendix A Replacement Parts and Accessories for part numbers If you have not already done so install the UniPoint System Software Refer to the installation instructions included with the software Example files for controlling the 845Z Injection Module in an analytical system are provided with UniPoint upon installation C Gilson UniPointNini 8452 Example files for controlling the 845Z Injection Module in a preparative system are supplied on the 845Z Injection Module Documentation CD part number LT2575CD Copy the folder 845ZPrep to your C Gilson UniPoint folder 3 1 Start Up Operation 3 2 INJECTION MODULE Start Up To get the injection module ready for injections turn power on to the 845Z control unit using the power switch located on the rear panel see diagram on page 2 4 Front Panel A SOLUTIONS AT WORK FOR YOU The front panel of the 845Z control unit has a green POWER light and an amber one character display When power is switched on the POWER light is lit The display identifies the status of
10. probe tip to valve using the equipment described in Appendix D Preparative 202 uL from probe tip to valve using the equipment described in Appendix D Dead volume depends on probe volume and calibrated tubing volume Environmental conditions Indoor use Altitude up to 2000 m Temperature range 5 40 C Air pressure 75 105 kPa Pollution degree 1 or 2 in accordance with IEC 66 Humidity Maximum relative humidity 80 for temperatures up to 31 C decreasing linearly to 50 relative humidity at 40 C Front panel One character LED display and power light suonpouo ds jesruy2 L Technical Specifications ntroduction Injection carryover Analytical 0 02 using the equipment and method described in Appendix D Preparative lt 0 01 using the equipment and method described in Appendix D Injection reproducibility Analytical Total loop fill CV 0 5 using the configuration and test procedure described in Appendix D Preparative Prep total loop fill no overfill CV lt 1 0 using the configuration and test procedure described in Appendix D Liquid contact materials Stainless steel PEEK Teflon Manufacturing standards Meets applicable Safety and EMC certification standards UL and CE certified Power requirements 24V DC at 2 0A supplied via a 2 1 mm ID power plug center contact is positive Software control Computer control via GSIOC and UniPoint
11. the valve L for load or I for inject Operation 3 Injection Technique Total Loop Fill With a total loop fill injection you must inject a total volume equal to two to three times the volume of the injection loop plus the dead volume The following diagrams describe the sequence of events for a total loop fill to syringe to syringe pump pump from probe fror prope port 1 Pa loop loop mobile phase mobile phase to column detector mobile phase pumps sample from the loop to the column Total Loop Fill Load left and Inject right Valve Positions 3 3 uon lul Operation uolun 4009 Ep o duind wou seud liqoul ainpow uono lul 2978 uled MO 4 pinbrq JUSAJOS 34 Maintenance 4 To obtain optimum performance and maximum life from the injection module it is important to keep the instrument vvell maintained In order to keep your injection module at peak performance Gilson recommends that you do the following e Flush the valves and tubing daily with distilled or deionized water e Periodically check the fittings to ensure that they are tight e Wipe up all spills immediately e Warm all fluids to room temperature before running them through the system cold fluids may cause leakage 4 1 Replacing the Rotor Seal and Stator Face Assembly Maintenance F Rotor Seal Stator Ring Stator Face Assembly
12. tip capacitive level sensing stainless steel Dimensions 221 5 x 1 5 x 1 1 mm ID tip dimensions 10 x 0 7 x 0 4 mm ID Requires probe holder guide kit part number 253641 Rinse station insert part number 25245533 for non level sensing applications deep pocket 1 mL syringe part number 25025343 1200 uL FEP transfer tubing part number 49942261 0 030 ID x 0 062 OD x 103 7 845Z Injection Module RV700 100 Rheodyne valve with PEEK rotor seal part number 496775055 20 uL stainless steel loop part number 49677522 18 cm calibrated Teflon tubing part number 49944182 from probe to valve port 6 TFE Tubing part number 495034 0 3 mm 0 010 ID x 1 5 mm OD 1 16 for connection between port 4 on the injection valve and the column or detector and between port 3 on the injection valve and the pump purge valve Appendix D Injection Carryover Method HPLC pump 322 Pump e H1 pump head Detector Fluorescence Detector e Gain 100 e Attenuation Sensitivity 16 e Emission 400 nm e Excitation 254 nm Mobile phase rinse solvent Isocratic e 100 methanol Flow rate 2 0 mL min Cycle time 26 seconds per injection Injection Total loop injection 2 to 3 times loop overfill dead volume of 2 ug mL anthracene in methanol Dead volume 40 uL Probe volume 28 uL Calibrated tubing volume 12 uL Rinse Probe at rinse station aspirate 400 uL from reservoir at 20 mL min then dispense 500 u
13. 0 mL min Injection reproducibility calculation CV Standard deviation for injection set 100 Average area for injection set Appendix D Preparative System 215 Liquid Handler 125 mm Z arm clamped at 125 mm Beveled tip probe part number 27067382 stainless steel grooved septum piercing Dimensions 221 x 2 0 x 0 8 mm ID Requires probe holder guide kit part number 253640 Rinse station insert part number 25245533 for non level sensing applications deep pocket 10 mL syringe part number 25025345 1200 uL FEP transfer tubing part number 49942261 0 030 ID x 0 062 OD x 103 7 845Z Injection Module RV700 112 Rheodyne valve 5 mL stainless steel loop part number 49677529 18 cm calibrated Teflon tubing part number 499421821 from probe to valve port 6 PEEK tubing part number 49954059 0 040 1 0 mm ID for connection between port 4 on the injection valve and the column or detector and between port 3 on the injection valve and the pump purge valve D 7 spoy N suonpouio ds Specifications Methods Appendix Injection Carryover Method HPLC pump 333 334 Pump e H3 pump head Detector 152 UV VIS Detector Flow cell 0 05 mm e Wavelength 254 e Sensitivity 0 01 e Peak width 10 sec Mobile phase rinse solvent e Gradient e 70 methanol e 30 water Flow rate 20 mL min Injection Prep total loop injection loop fill 100 uL of 55 000 ng mL ethyl paraben in 70 30 methanol
14. 3S pue je s J0 OY v Huloejdeay Troubleshooting 5 Error Messages When an error has occurred the error number will flash alternating with on the LED display on the 845Z control unit Refer to the table below for the list of errors and possible resolutions Error Description Solution 1 Positioning error e The hex screws may be too tight in the stator Loosen with a 9 64 Allen head screwdriver e The position sensor may need to be cleaned For assistance contact the Gilson Customer Service Department e Valve is defective or absent replace valve e Sensor is defective replace sensor For assistance contact the Gilson Customer Service Department 2 GSIOC command to move Turn power OFF then ON to the injection module valve is received when the valve is not homed 3 GSIOC command to move e Turn power OFF then ON to the injection module valve is received while the valve is in operation e Make changes to the program controlling the valve 4 A buffered GSIOC e Turn power OFF then ON to the injection module command was sent with an incorrect parameter e Resend the command with a valid parameter 5 Problem with connection Verify that cables are properly installed and or cabling connected 5 1 Electrical Troubleshooting 5 2 Electrical Unit not operational Make sure povver is turned on Check AC povver cord connections Try different AC outlet Check all connections nput functions not opera
15. 845Z Injection Module User s Guide LT2575 2004 Gilson Inc All rights reserved May 2004 Table of Contents Declaration of Conformity 1 Introduction 2 Installation 3 Operation 4 Maintenance Unpacking Customer Service Technical Specifications Installing the Injection Module Removing the Tubing Support Rod Mounting the 845Z Injection Module Installing the 845Z Control Unit Making Electrical Connections 845Z Control Unit Rear Panel Unit ID Selection GSIOC Port Input Output Port Power Connections Making Plumbing Connections Sample Loop Column and Pump Tubing Syringe Pump and Probe Tubing Start Up Front Panel Injection Technique Total Loop Fill Liquid Flow Path Replacing the Rotor Seal and Stator Face Assembly 4 2 5 Troubleshooting AppendixA Appendix B Appendix C Appendix D Error Messages Electrical Mechanical Repair and Return Policies Replacement Parts and Accessories GSIOC Commands eee eer cect aes eae ea tenn B 1 GSIOC Command List Baud Rate Specifications Methods Analytical Injection Carryover Method Injection Reproducibility Method Preparative Injection Carryover Method Injection Reproducibility Method Declaration of Conformity Application of Council Directives 89 336 EEC 73 23 EEC Standards to vvhich Conformity is Declared EN61326 EN61000 3 3 EN61000 3 2 EN61010 1 2001 Manufacturer s Name Gilson Inc Manufacturer s
16. Address 3000 W Beltline Highway Middleton WI 53562 EC Office Address Gilson S A S 19 Avenue des Entrepreneurs B P 145 F 95400 Villiers le Bel France Type of Equipment Laboratory Equipment Models r PP U UI nn een ee enor 845Z Injection Module Beginning with Serial Number 250C1N001 Month and Year of Manufacture March 2001 I the undersigned hereby declare that the equipment specified above conforms to the above Directives and Standards Place Middleton WI USA Michael Jacquart Issue Date February 2001 Senior Vice President Corporate Technology Development Introduction 1 The Gilson 845Z Injection Module enables you to inject samples onto an HPLC or other analytical or preparative system It is used with a Gilson 215 Liquid Handler 845Z Injection Module on a 215 Liquid Handler 1 1 Unpacking ntroduction Unpacking The injection module is delivered with all major components already assembled Keep the original container and packing material in case the module must be returned to the manufacturer The injection module and accessories are shipped in one container Unpack the container and inspect for damage Promptly report any damage to the carrier Some carriers must receive concealed damage claims within seven days of delivery After unpacking ensure you have one of the following injection modules and its accessories Part Number Descri
17. L 400 uL rinse volume 90 uL sample volume 10 uL air gap at 20 mL min D 3 spou ll suoneouo ds Specifications Methods Appendix Injections 1 Two injections of methanol blanks 2 One injection of 2 ug mL anthracene in methanol 3 Two injections of methanol blanks Repeat steps 2 and 3 ten more times Calibration curve parameters e External calibration e Linear curve fit e Five calibration levels 0 4 ng mL anthracene in methanol 1 0 ng mL anthracene in methanol 2 0 ng mL anthracene in methanol 6 0 ng mL anthracene in methanol 10 0 ng mL anthracene in methanol Or Injection protocol Total loop injection Initialize your system i e home syringe at rinse station and home XYZ arm before running any method Set injection valve to load Aspirate 10 uL air gap at 0 3 mL min Move to sample location 15 mm from bottom Aspirate 90 uL sample volume 2 to 3 times loop overfill dead volume of 2 ng mL anthracene in methanol at 5 mL min Wait 0 02 minutes to equilibrate Set injection valve to inject Coordinate with start of data collection Move to outside rinse station Rinse probe aspirate 400 uL from reservoir at 20 mL min then dispense 500 uL 400 uL rinse volume 90 uL sample volume 10 pL air gap at 20 mL min H Go M VO O 01 Appendix D Injection Reproducibility Method HPLC pump 322 Pump e H1 pump head Detector Fluorometer e Gain 100 e Attenuation Sensiti
18. Refer to the diagram on page 2 4 for the location of the input output port The 845Z Injection Module has one contact input Input Action open to close valve to Inject position close to open valve to Load position The module has one contact output The contact is closed when the valve reaches the Inject position The contact is opened when the valve reaches the Load position While the valve is moving the contact remains in the previous state When using contact input control if input equals output motion is finished Output State open Load position closed Inject position suon uuo ip u lzi Bubiely Making Electrical Connections Installation 2 8 2 conductor cable terminal block connector to peripheral equipment Making connections To make connections you need the follovving e 2 conductor cable 22 30 gauge for each wire e wire insulation stripper e small blade screwdriver You can purchase a 6 foot piece of suitable cable part number 709910206 from Gilson To prepare and make connections with the 2 conductor cable 1 Cut the cable into pieces of appropriate length 2 Strip about 0 25 cm of insulation from each end of the cable 3 Locate the terminal block connector in the accessory package 4 Insert each wire into the appropriate slot on the connector Push the wire all the way in then tighten its corresponding pin screw 5 Connect the terminal block connector to the
19. ample loop stainless steel 100 uL sample loop stainless steel 1 mL sample loop stainless steel 2 mL sample loop stainless steel 5 mL sample loop stainless steel TFE Tubing 0 3 mm 0 010 ID x 1 5 mm OD 1 16 package of 10 ft Tubing 040 x 5 ft PEEK Rheodyne 6000 055 package of 5 RheFlex short fitting set 5 nuts 5 ferrules A 1 Replacement Parts and Accessories Appendix 49942261 49041028 49041031 49944182 499421821 541180372 A 2 1200 uL FEP tubing 0 030 ID x 0 062 OD x 103 7 Upchurch P 742 union 0 1 to 10 32 thread P 706 PEEK ZDV union 050 thru hole 18 cm Teflon tubing with fittings for connection from probe to valve port 6 Tubing 8 mm ID x 7 2 w end fitting Spiral wrap Appendix Probes for 125 mm Z arm 2507234 2507235 2507236 2507227 2507242 2507243 2507244 2507245 Septum piercing probe side entry capacitive level sensing stainless steel strain relief design Dimensions 274 x 1 8 x 0 8 mm ID tip dimensions 12 x 1 3 mm OD entry hole center is 3 5 mm from tip Requires probe holder guide kit part number 253640 Septum piercing probe side entry vented capacitive level sensing stainless steel strain relief design Dimensions 274 x 1 8 x 0 8 mm ID tip dimensions 12 x 1 3 x mm OD entry hole center is 3 5 mm from tip Requires probe holder guide kit part number 253640 Septum piercing short pencil point probe vented at 14 m
20. and turn it 2 Align the arrow head with one of the indicated numbers The unit ID is 20 plus the selected number 7 4 ANI Unit ID 20 setting 2 5 suon uuo2 2911399 Buryjeyy Making Electrical Connections Installation 2 6 9 pin female connector connect to GSIOC port of injection module GSIOC Port Use the GSIOC port to connect the injection module to the liquid handler This enables the module to be controlled by a program running on the computer connected to the liquid handler 1 Locate the GSIOC cable in the accessory package 2 Connect one of the female 9 pin connectors to the GSIOC port of the injection module 3 Connect the other female 9 pin connector to the GSIOC port of the liquid handler If the liquid handler and injection module are part of a Gilson system use the male connector to connect additional instruments along the GSIOC Refer to the installation guide supplied with the Gilson system software mi lt 30 gt 9 pin female connector connect Jye 4 gt to liquid handler E 5 T T 9 pin male connector connect to GSIOC cable for linking additional GSIOC modules Installation Input Output Port You can use the input and output contacts found on the rear panel of the 845Z control unit to receive signals from and send signals to other instruments
21. cabinet support Z arm rod Z axis 2 Use the supplied 7 64 Allen wrench to remove the four Allen screvvs securing the tubing support rod to the control cabinet from inside the cover 3 Store the tubing support rod 4 Replace the control cabinet cover on the control cabinet using the six screvvs you removed in step 1 Installation 2 Mounting the 845Z Injection Module Important Before mounting the 845Z Injection Module on the 215 Z arm remove the low pressure valve assembly and mounting bracket if installed 1 Using a Phillips screwdriver loosen the mounting screw on the mounting bracket located on injection module Turn counterclockwise to loosen 2 Partially pull out the bracket Do not remove completely 3 Mount the injection module on the left top side of the Z arm You will need to mount one side of the injection module at a time front to back The valve should face the front and the mounting bracket should face the right Note It is possible to optimize your tubing length by mounting the injection module lower on the Z arm However do not mount the arm at any lower than 40 mm per the scale on the side of the Z arm Doing so may damage the Z arm control cable port on the liquid handler 4 Tighten the screw on the mounting bracket until the injection module is secure on the Z arm Installing the 845Z Control Unit Place the 845Z control unit on the 215 control cabinet It is best to p
22. e the serial number located on bottom of the control unit of your injection module e the installation procedure you used e list of concise symptoms e list of operating procedures and conditions you were using when the problem arose e list of other devices connected to the multiple injection module and a description of those connections e list of other electrical connections in the room Warranty repair Units covered under warranty will be repaired and returned to you at no charge If you have any questions about applicability please contact the Gilson Customer Service Department or your authorized representative Non warranty repair Contact your local Gilson representative or the Gilson Customer Service Department to discuss service options and make arrangements to return the instrument if necessary Rebuilt exchange For some units rebuilt exchange components are available Contact the Gilson Customer Service Department for details 5 5 s r olod pue ed Repair and Return Policies Troubleshooting Return procedure In the United States contact the Gilson Customer Service Department to obtain authorization before returning any Gilson equipment To return a piece of equipment e Carefully pack the unit to prevent damage in transit Check with Gilson regarding proper method of shipment No responsibility is assumed by Gilson for damage caused by improperly packaged instruments Indicate the a
23. eflon tubing supplied in the plumbing package from your pump to port three of the injection valve Connect the 0 3 mm ID Teflon tubing supplied in the plumbing package from port four of the injection valve to your column detector A PEEK union is supplied in the plumbing package for use if necessary Preparative Connect the 0 040 1 0 mm ID PEEK tubing supplied in the plumbing package from your pump to port three of the injection valve Connect the 0 040 1 0 mm ID PEEK tubing supplied in the plumbing package from port four of the injection valve to your column detector A PEEK union is supplied in the plumbing package for use if necessary Installation 2 Syringe Pump and Probe Tubing from inlet to injection valve tubing port 1 valve screw valve to vent tubing syringe H piston piston operating rod piston holding screw suon uuo Ie u l i Bubieiy Analytical Connect the 1200 uL 0 8 mm ID tubing supplied in the plumbing package from port one of the injection valve to the outlet right port on your syringe pump Connect the 0 3 mm ID calibrated Teflon tubing pre cut to 18 cm and supplied in the plumbing package from the top of the probe holder to port six of the injection valve Preparative Connect the 1200 uL 0 8 mm ID tubing supplied in the plumbing package from port one of the injection valve to the
24. eservoir at 100 mL min then dispense 10 000 uL at 20 mL min Rinse probe aspirate 10 000 uL from inside reservoir at 100 mL min then dispense 10 000 uL at 20 mL min D 9 spoy N suonpouio ds Specifications Methods Appendix D 10 Injection Reproducibility Method HPLC pump 333 334 Pump e H3 pump head Detector 152 UV VIS Detector Flow cell 0 05 mm e Wavelength 254 e Sensitivity 0 1 e Peak width 10 sec Mobile phase rinse solvent e Gradient e 70 methanol e 30 water Flow rate 20 mL min Cycle time 70 seconds per injection full loop Load to Inject 43 seconds post injection rinsing Injection Prep total loop injection loop fill pull volume 190 uL of 550 pg mL ethyl paraben in 70 30 methanol vvater Dead volume 202 uL Probe volume 111 uL Calibrated tubing volume 91 uL Rinse Probe at rinse station aspirate 2000 uL from reservoir at 10 mL min then dispense 2000 uL at 10 mL min Appendix D Injections 1 2 One system blank 25 injections of 550 ug mL ethyl paraben in 70 30 methanol water Five injections per sample tube Injection protocol Prep total loop injection Initialize your system i e home syringe at rinse station and home XYZ arm before running any method 1 Set injection valve to load 2 Aspirate 100 uL air gap at 1 0 mL min 3 Move to sample location 2 mm from bottom 4 Aspirate 5000 uL sample volume loop fill of 13 550 ug mL ethyl pa
25. lace it toward the rear as you will need to make electrical connections to the unit 2 3 lnpoyy UoNsafuy y Sullle sul Making Electrical Connections Installation 2 Making Electrical Connections 845Z Control Unit Rear Panel 6 LISTED TO db CERTIFIED TO he 845Z 4 vss Ius sto 10101 4p GILSON INJECTION MODULE 0992535620 3000 WEST BELTLINE HIGHWAY BOX 620027 ACN 001 768 396 MIDDLETON WI 53562 0027 THIS DEVICE COMPLIES WITH PART 15 OF THE FCC RULES OPERATION IS SUBJECT TO THE FOLLOWING TWO CONDITIONS 1 THIS DEVICE MAY NOT CAUSE HARMFUL INTERFERENCE AND 2 THIS DEVICE MUST ACCEPT ANY INTERFERENCE RECEIVED INCLUDING INTERFERENCE THAT MAY CAUSE UNDESIRED OPERATION CE ial 1 0 iz GSIOC UNIT ID POWER H POWER INPUT OUTPUT 20 SETTING TES 0 Baza 1 Input Output I O port 2 Gilson Serial Input Output Channel GSIOC port 3 Unit ID selector 4 Power switch 5 Power receptacle 6 Power outlet to injection module 24 Installation Unit ID Selection At the factory Gilson set the unit ID to 29 The unit ID identifies the injection module to Gilson software packages that can issue GSIOC commands to Gilson instruments There is no need to change the unit ID unless it is the same as that assigned to another Gilson instrument connected along the GSIOC To change the unit ID 1 Gently insert a small flat blade screwdriver into the Unit ID selector on the rear panel
26. m side entry vented at 72 mm capacitive level sensing stainless steel Dimensions 272 x 1 8 x 0 8 mm ID Requires probe holder guide kit part number 253640 Septum piercing pencil point probe vented at 72 mm side entry capacitive level sensing stainless steel Dimensions 273 x 1 8 x 0 8 mm ID Requires probe holder guide kit part number 253640 Septum piercing probe deflected tip capacitive level sensing stainless steel with Teflon coated liquid path strain relief design Dimensions 274 x 1 8 x 0 8 mm ID tip dimensions 12 x 1 3 mm OD bevel cut entry hole Requires probe holder guide kit part number 253640 Septum piercing probe deflected tip vented capacitive level sensing stainless steel with Teflon coated liquid path strain relief design Dimensions 274 x 1 8 x 0 8 mm ID tip dimensions 12 x 1 3 mm OD bevel cut entry hole Requires probe holder guide kit part number 253640 Septum piercing probe deflected tip capacitive level sensing stainless steel strain relief design Dimensions 274 x 1 8 x 0 8 mm ID tip dimensions 12 x 1 3 mm OD bevel cut entry hole Requires probe holder guide kit part number 253640 Septum piercing probe deflected tip vented capacitive level sensing stainless steel strain relief design Dimensions 274 x 1 8 x 0 8 mm ID tip dimensions 12 x 1 3 mm OD bevel cut entry hole Requires probe holder guide kit part number 253640 A 3 S HOSS Vy pure
27. ng parameters for control operation of or request information from the injection module GSIOC Commands Appendix B 2 GSIOC Command List There are two kinds of commands that you can send over the GSIOC e Buffered commands B send instructions to the injection module These commands are executed one at a time e Immediate commands I request status information from the injection module These commands are executed immediately temporarily interrupting other commands in progress In the command list below the GSIOC command must be entered in the proper upper or lower case format If a buffered command requires additional information you see italicized text next to the command The description of the command identifies what you need to enter in place of the italicized parameter Appendix Command 70 Oaal xxxx Type I B Description Resets the injection module Equivalent to turning power OFF then ON Reads the current firmware version Returns 845Zvx xx where x xx indicates the currently installed version Reads non volatile memory NV RAM at current address Returns aa xxx where aa Value between 0 and 19 for word type data 0 Valve type xxx Data at the address for Valve type 0 0 RV700 100 Analytical 0 1 RV700 112 Prep Sets the value at NV RAM address where aa Value between 0 and 19 for word type data or 100 to 119 for floating type data xxxx Optional Data
28. ption Analytical 251591 845Z Injection Module with control unit and installed Rheodyne RV700 100 valve and sample loop includes GSIOC cable 7 64 Allen wrench power cords terminal block connector 845Z Documentation CD 250550152 845Z plumbing package includes tubing fittings 5 16 1 4 wrench PEEK union spiral wrap and tubing clips Preparative 251552 845Z Prep Injection Module with control unit and installed Rheodyne RV700 112 valve and sample loop includes e GSIOC cable 7 64 Allen wrench power cords terminal block connector 845Z Documentation CD 250550252 845Z Prep plumbing package includes tubing fittings 5 16 1 4 wrench PEEK union spiral wrap and tubing clips ntroduction Customer Service Gilson Inc and its vvorldvvide netvvork of authorized representatives provide you vvith the follovving types of assistance sales technical applications and instrument repair If you need assistance and are in the United States please contact your regional Gilson representative or call the Gilson Customer Service Department at 800 445 7661 or 608 836 1551 You can also contact the Gilson Customer Service Department via its e mail address service gilson com Outside the United States contact your Gilson representative for assistance Specific contact information can be found on the Gilson web site at www gilson com JOWIO SND Technical Specifications ntroduction
29. raben in 70 30 methanol water at 5 mL min Move arm to top Aspirate 190 uL pull volume Wait 0 01 minutes to equilibrate Coordinate with start of data collection Set injection valve to inject Move to outside rinse station Home syringe Rinse probe aspirate 2000 uL from outside reservoir at 10 mL min then dispense 2000 uL at 10 mL min Rinse probe aspirate 2000 uL from inside reservoir at 10 mL min then dispense 2000 uL at 10 mL min Injection reproducibility calculation CV Standard deviation for injection set 100 Average area for injection set D 11 spoy N suonesyoadg
30. tainless steel Dimensions 221 x 1 5 x 1 1 mm ID tip dimensions 2 x 1 1 x 0 4 mm ID Requires probe holder guide kit part number 253641 Non septum piercing probe beveled tip Teflon coated stainless steel Dimensions 221 x 1 5 x 1 1 mm ID Requires probe holder guide kit part number 253641 Beveled tip probe stainless steel grooved septum piercing Dimensions 221 x 2 0 x 0 8 mm ID Requires probe holder guide kit part number 253643 Grooved septum piercing probe beveled tip stainless steel Dimensions 221 x 1 5 x 0 4 mm ID Requires probe holder guide kit part number 253641 GSIOC Commands B What is GSIOC The Gilson Serial Input Output Channel GSIOC is an asynchronous serial communications interface that enhances the power of your Gilson equipment The GSIOC incorporates an EIA RS 485 interface and allows up to 32 slave devices to be controlled from a single master in a multiple drop configuration Each slave device is identified by a unique number which must be known to the instrument and to the controller The default unit ID of the injection module is 29 To control the injection module via the GSIOC interface you need the following e acomputer with any Gilson control software or 706 Device Driver Software installed e 506C System Interface or 215 Liquid Handler From the computer you e specify the unit ID of the injection module as the device you want to control e issue commands that set operati
31. ting Make sure connections into terminal block connector are secure Make sure terminal block connector is secure in input output port Check connections for proper pin assignments Be sure pins from external devices are assigned correctly Check polarity of input Inputs should be a contact closure If not it must be TTL level logic 0 activates Confirm that source supplying input to injection module is working Troubleshooting Output functions not operating Make sure connections into terminal block connector are secure Make sure terminal block connector is secure in the input output port Check connections for proper pin assignments Output from injection module should be compatible with device to which it is interfaced Outputs are contact closures 5 3 jeo14 99 5 Mechanical Troubleshooting Mechanical Mobile phase pressure is steadily increasing e Check that the injection valve inlet tubing from the pump port 4 and or outlet tubing to the detector port 3 is not crimped or plugged Liquid leaking from the bottom of the injection valve Check that all fittings are secure in the valve tighten if necessary Check that the stator face is securely tightened to the valve tighten if necessary Troubleshooting 5 Repair and Return Policies Before calling us Gilson Customer Service personnel vvill be able to serve you more efficiently if you prepare the following information
32. uthorization on the carton and on the packing slip e Always insure for the replacement value of the instrument e Include a description of symptoms your name address phone number and purchase order to cover repair costs return and shipping charges if your institution requires it Ship to Gilson Inc Attention Customer Service indicate the authorization here 3000 W Beltline Highway Middleton WI 53562 Outside the United States contact your Gilson representative for return procedures Replacement Parts and Accessories Part Number 21064651 496700100 496775038 496775027 496775055 496700112 4967001121 4967001122 36078143 49677520 49677521 49677522 49677523 49677524 49677527 49677528 49677529 495034 49954059 49656055 A Description UniPoint LC System Software V5 1 Chromatography software package for data acquisition and control of Gilson HPLC systems fraction collectors and automated switching valves ERM capabilities and features facilitate 21 CFR Part 11 compliance Requires Microsoft Windows 2000 SP3 or XP Professional SP1 and 506C interface module RV700 100 Rheodyne valve RV700 100 stator RV700 100 stator face assembly RV700 100 rotor seal RV700 112 Rheodyne valve RV700 112 stator RV700 112 stator face assembly and rotor seal Shielded GSIOC cable 30 5 HL sample loop stainless steel 10 uL sample loop stainless steel 20 uL sample loop stainless steel 50 uL s
33. vity 16 e Emission wavelength 400 nm e Excitation wavelength 254 nm Mobile phase rinse solvent Isocratic e 100 methanol Flow rate 2 0 mL min Cycle time 26 seconds per injection Injection Total loop injection 2 to 3 times loop overfill dead volume of 6 ng mL anthracene in methanol Dead volume 40 uL Probe volume 28 uL Calibrated tubing volume 12 uL Rinse Probe at rinse station aspirate 400 uL from reservoir at 20 mL min then dispense 500 uL 400 uL rinse volume 90 uL sample volume 10 uL air gap at 20 mL min D 5 spou ll suoneouo ds Specifications Methods Appendix Injections 1 Two methanol blank injections 2 Ten injections of 6 ng mL anthracene in methanol 3 Two methanol blank injections Injection protocol Total loop injection Initialize your system i e home syringe at rinse station and home XYZ arm before running any method 1 Set injection valve to load 2 Aspirate 10 uL air gap at 0 3 mL min 3 Move to sample location 15 mm from bottom 4 Aspirate 90 uL sample volume 2 to 3 times Vo CON OD V1 loop overfill dead volume of 6 ng mL anthracene in methanol at 5 mL min Wait 0 02 minutes to equilibrate Set injection valve to inject Coordinate with start of data collection Move to outside rinse station Rinse probe aspirate 400 uL from reservoir at 20 mL min then dispense 500 uL 400 uL rinse volume 90 uL sample volume 10 pL air gap at 2
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