USER GUIDE
Contents
1. Single 8 channel unit User guide Certificate of conformity AC DC power adaptor Hanger and accessories Pipette tips extra battery If there are any items missing damaged or not according to your order please contact our distributor or sales representatives for replacement immediately Please charge up the battery before first time operation Note The items inside the package may be subject to change according to your requests 3 2 Inserting the Battery Remove the battery cover by pressing its latch and tilting it to the side see Figure 1 Insert the battery see Figure 2 with the metallic contacts facing downwards Close the cover of the battery compartment Figure 1 Open the battery cover Figure 2 Insert the battery Note After the battery is inserted the pipette will automatically carry out a calibration routine 3 3 Charging the Battery Internal Charging Circuit Insert the battery into the battery compartment Insert the DC in plug of the power adaptor into the DC in jack see Figure 3 Connect the power plug of the power adaptor with the external power source Connecting the power plug before inserting the battery will inactivate the charging process The indication bar inside battery symbol Wn will blink during the charging process When the battery is fully charged the blinking will stop and the pipette will beep once to remind the user Figure 3 Using the int2. 4 Overview This section presents an overview of the CappTronic pipette see Figure 6 and points out the locations of its buttons The LCD display panel is also shown below see Figure 7 N LCD Display Keypad Battery Cover an RESET Button gt Tip Ejector START Button X ES Finger Rest Bushing N Connecting Nut Lower Part Tip Fitting Front View Side View Figure 6 1 channel pipette overview sys System Setting auto Auto Pipetting mp Multiple Dispensing mx Mixing Count No Prog No se Sequential Aspirating Dispensing Dan y Volume ul DW EN SYS AUTO MD MIX SE Direction AY COUNT iQ 16 Acoustic Signals Spoed BB 1000 a A Tip Eon Alii Battery Capacity Eg EN Me vii MA BlowOu we Reverse Pipetting EXIT Button SET Button Up Down Button Figure 7 LCD display Panel 4 1 Keypad Function Button Symbol Function Description Set Enter Confirms your choice Exit Esc q Exits to previous mode setting Up y Increases volume changes parameter Down Y Decreases volume changes parameter Piston automatically determines the Reset home position Activates aspirating dispensing Start START Replace ff button Tip Ejector TIP Ejects the tips Tips On Efficient Operation START button can be used to increase volumes and to change modes and settings as Up button during the function
3. 7 function settings are sequential procedures To store these settings in memory you have to complete the whole setting procedure and press SET when the LCD displays PROG 2 Youcanuse the 9 Up Down button to move back and forth to the previous function settings 6 1 Aspirating Speed There are 5 speeds available for both aspirating and dispensing 1 Press EXIT to enter the function mode selection Then press gt Up Down repeatedly until the SYS symbol starts flashing 2 Press D SET to confirm the selection of SYS mode and the aspirating speed symbol will start flashing 3 Press Up Down to change the aspirating speed Slowest E Fastest 4 Press D SET to confirm the speed selection and move to the Dispensing Speed function setting 6 2 Dispensing Speed x 1 The Dispensing Speed symbol should now be flashing 2 Press Up Down to change the aspirating speed Slowest Fastest 3 Press D SET to confirm the speed selection and move to the Acoustic Signals function setting 6 3 Acoustic Signals Please refer to Section 4 2 for the definition of Acoustic Signals 1 The Acoustic Signals symbol 16 should now be flashing 2 Press Up Down to select the preference 14 On Off 3 Press D SET to confirm the selection and move to the Tip Ejection Alarm setting 6 4 Tip Ejector Alarm The tip ejector ala
4. DC 3 6 V Acoustic Indication Yes Autoclavable Yes for lower part only Operating Temperature 5 50 C Operating Humidity RH 0 85 Battery 900 mAh 3 6 V or above Stand for 3 units Optional Certification Complies with CE Class A EN60101 1 2 EN50082 1 EN 55011 Complies with ISO 8655 DIN 12650 Note 1 U S patents pending 2 Specifications are subject to change without prior notice Appendix B Tip Ejector Height Adjustment 1 Remove the lower part of pipette as described in Section 11 1 2 Remove the flat head screw and bushing as shown in Figure 14 3 Use flat head screw driver or other tool to rotate the tip ejector height adjustment nut By rotating the nut in counter clockwise direction the tip ejector will move closer to the mouth of tip fitting This height adjustment will enable the pipette to accommodate to a wider range of tips 26 Tip ejector height an adjustment nut Flat head screw Figure 14 Tip ejector height adjustment Appendix C Warranty The CappTronic electronic pipettes are covered by a warranty for one year against defects in materials and workmanship This period begins from the date of purchase and within this period all defective parts will be replaced The warranty does not cover defects caused by excessive tear and wear or damage due to shipping accident abuse misuse problems with electrical power or usage not in accordance with product instruction
5. lower part of the pipette 1 Press the START button or any button of the keypad to make the step motor shaft retract inside of upper part for 1 cm The step motor will automatically retract inside of upper lower part if users do not touch any button for 10 minutes 2 Connect the lower part with connecting nut Tighten up the connecting nut 3 Press the button The step motor shaft will connect with the piston of the lower part automatically After the connection is completed the pipette will beep twice 12 Maintenance The outside of the pipette may be wiped clean with 60 Isopropanol 7096 ethanol or a suitable detergent such as Trigene and then wiped with a lint free cloth If the pipette is severely contaminated or if very corrosive chemicals are dispensed the lower part of the pipette should be disassembled and rinsed The individual parts should be decontaminated with a suitable detergent rinsed in distilled water and then air dried It is recommended to clean the pipette at regular intervals depending on how much the pipette is used as well as lubricating it once per year If the pipette is heavily used or autoclaved often you may need to lubricate the pipette more frequently Only Capp trained representatives or users are recommended to lubricate the piston Please use the lubricant as offered by Capp only 23 It is recommended to check the performance of your pipette regularly e g every 3 6
6. mode selection and volume settings This unique design will speed up the setting processes 4 2 Acoustic Signals The acoustic signals are a great help when you are familiarizing yourself with the operating procedure of the CappTronic pipette They can also be switched off if required see Section 6 3 Acoustic Sound Operation Low tone beep Aspirating High tone beep Dispensing Tip Ejection Error codes MD MIX SE mode cycle or RESET routine is 2 beeps completed 5 Function Map The main menu of the pipette includes five function modes SYS AUTO MD MIX and SE Each mode includes various function settings see Figure 9 Function Map Mode Aspirating a mood Dispensing Mix Speed Dispensing Number Number Volume COUNT COUNT Dispensing Dis i i ispensing Mix Dispensing Volume Nimhe 1 2 3 Acoustic Signals Dispensing Function as Settings Tip Ejection Alarm Aspirating Number Blow out 1 2 3 Reverse Aspirating Pipetting Volume Program Recall Figure 8 Function map 6 SYS System Mode SYS mode is used to set up the function settings before pipetting Once users select and store these settings they will no longer need to set up the settings before every pipetting There are 7 function settings Aspirating Speed Dispensing Speed Acoustic Signals Tip Ejection Alarm Blow out Reverse Pipetting and Program Recall in SYS mode 11 Note 1 These
7. symbol e should now be flashing 2 Press Up Down to choose the preference On Off 3 Press D SET to confirm the selection and move to the PROG setting Note If reverse pipetting is selected you will have to press the STAR button one more time to dispense the remaining liquid during the pipetting r E will appear on the LCD to remind users to dispense the remaining liquid 6 7 Setting PROG Programs The memory program includes 9 different storage locations PROG 01 09 Your favorite operating modes with user selected settings can be stored to these locations for future recalls Before setting the programs you must have programmed operating mode e g AUTO MD mode etc 14 Store Operating Mode to Storage Locations 1 Complete the setup of function mode e g AUTO MD MIX mode Press and hold W Down for 3 seconds to enter PROG mode Press Up Down to select PROG number from 01 09 Bow B Press D SET to confirm the location selection and save the functions Recall Stored Programs from the Storage Location 1 Press EXIT to enter the function mode selection Then press S Up Down repeatedly until the SYS symbol starts flashing 2 Press D SET repeatedly to confirm the selection of all 6 function settings until the PROG symbol starts flashing 3 Press Up Down to select the PROG number 4 Press D SET to confirm the selection 5 Press th
8. CappTronicv Single and Multichannel Electronic Pipettes USER GUIDE Version 2 9 Copyright No part of this user guide may be reproduced or transmitted in any form or by any means electronic or mechanical for any purpose without prior written permission of Capp A S CappTronic and Capp are trademarks of Capp A S Table of Contents 1 Safety Precautions ssrcssscssensessorssuessnssnsssuenunsssnennensnensnsssensoesssnssnssesssnenen 1 1 1 Operation Safety Precautions seen 1 1 2 Battery Safety rata eg a iie boards 1 2 General Description orscorsonsennssnsssnsesnnensnssnssonssonssnnssnssonssonsnonsnnssnnsenne 3 2 1 Features si ic 3 3 AA TT 4 3 1 Unpa cking mee eR EE 4 3 2 Inserting the Battery sese 4 3 3 Ch rgmg the Battery 5i ee hee e rh en 3 3 4 Start Pipettin ardid ti 6 3 5 Hanger Installation sucias aa 7 4 OVervieW 8 4 1 Keypad Functi0ns 3 i dies bi Ea deret e bet 9 4 2 Acoustic Sipnals re en e rpm PERDE 10 5 Function Map eoe etetinteoo tese top tate torre Ie PR E SUSS sess sescosnsetsoessbvaseesonsnen 10 6 SYS System Mode eerte eren eee eene tenens etna etas ta seta seta sete sees nato 11 6 1 Aspirating Speed ana Oed aei ec ee ats 12 6 2 Dispensing Speed ceri met te eee 12 6 3 Acoustic Signals asses gabe dba mtd 13 6 4 Tip Ejector Alarm acest b tee epe 13 6 5 BloW
9. arantee problem free safe operation of your new pipette it is essential to observe the following points 1 1 Operation Safety Precautions AN When using infectious radioactive toxic and other solutions which may pose health risks please observe the safety precautions laid down for your country Do not use the pipette in a potentially explosive environment or with potentially explosive chemicals When using organic solvents or corrosive chemicals please ensure that they are compatible with the pipette tips and the pipette Repair should be carried out by Capp authorized service personnel only Use original spare parts and accessories only 1 2 Battery Safety A The CappTronic pipette operates on a small but powerful Lithium ion battery Misuse or abuse of the Lithium ion battery may cause damage or injury through fire electric shock or chemical leakage Please read and understand all warnings before using the battery When storing the battery do not allow it to come into contact with any metallic surfaces Never use the pipette while using the DC in jack to charge the battery 1 11 12 Do not incinerate the Lithium battery or expose it to excessive heat Do not short circuit puncture crush disassemble damage deep cycle re vitalize or modify the battery Do not expose the battery to water or moisture Do not drop or subject the battery to strong impacts Only use the battery specified in this manua
10. e START button to operate the pipette in the selected program Note During the recall process the changes of other settings will not be stored 7 AUTO Mode In the AUTO mode the pipette performs the aspirating and dispensing of the fixed liquid volume 1 Press EXIT to enter the mode selection which causes the AUTO symbol to start flashing If not press S Up Down to select AUTO mode and make it flashing Press D SET to confirm the mode selection a re Use w Up Down to select the desired pipetting volume Press D SET to confirm selected volume and readiness for pipetting Tip You may use the START button to replace 44 Up to speed up the volume setting To have fast volume changes during pipetting works users can press D SET to make selected pipetting volme flash Use T Up Down to select the desired pipetting volume Press D SET again to confirm the desired pipetting volume 8 MD Multiple Dispensing Mode In MD mode the CappTronic performs repetitive dispensing of a selected volume To compensate the possible mechanical gap users MUST select Reverse Pipetting in System Mode and blow out the residual volume in the end of every multiple dispensing cycle If the reversed function is performed the sum of the dispensing aliquots and an automatically selected excess volume is aspirated into the tip The excessive volume is needed to ensure equal
11. ernal charging circuit to charge the battery Note 1 For safety concerns the pipette shall not be used during the charging period 2 Do not charge the battery for a long period of time 3 4 Start Pipetting There is no power On Off button on the pipette Once inserting the fully charged battery the pipette will be switched on It will perform a calibration routine and switch on the LCD display as in Figure 8 Press any keypad button or the START button to enter the function mode selection The pipette will be automatically switched off if it s not in use for more than 10 minutes Select the desired function mode and settings refer to the following chapters 6 10 before pipetting After the initial set up please attach the correct sized tips to the tip fitting tip base of the pipette before operation By simply 6 pressing the START button the pipette will start working The CappTronic is ergonomically designed Hold the pipette as shown in Figure 5 Use your index finger to press the START button and your thumb to press the ejector Figure 4 Correct operation 3 5 Hanger Installation The hanger can be fixed in multiple ways beside the experimental bench by screw or double adhesive tape inside accessory bag as shown in Figure 6 The finger rest can be attached to the hanger to keep the pipette in a vertical position LO Figure 5 Hanger installation 7
12. l Do not use a leaking battery Do not charge the battery for a long period of time The battery temperature rises with extended periods of use Care should be taken to avoid burns Burns may result if the battery is removed immediately after extended periods of use If fluid from the battery enters your eye immediately rinse the eye with plenty of fresh water and contact a doctor If fluid from the battery makes contact with your skin or clothing wash the area thoroughly with water 2 General Description CappTronic features an accurate and efficient as well as ergonomic and light weight design The pipette is designed to conduct various liquid handling tasks in the laboratory with a reduced risk of strain and upper limp disorders 2 1 Features High accuracy and precision Force savings Useful practical operation protocols such as Automatic Pipetting AUTO Multiple Dispensing MD Mixing MIX and Sequential Aspirating Dispensing SE etc Possibility of up to 9 user defined program settings 5 speeds for aspirating and dispensing Long operating hours Ergonomic design Automatic calibration Graphic operation interface as easy and user friendly as a mobile phone Adjustable tip ejector height Automatic keeping of the last used pipetting protocol and settings Complies with CE ISO 8655 GLP 3 Getting Started 3 1 Unpacking Open the package and check whether it contains the following items
13. luding air gap volume cannot exceed maximum volume range Press D SET to confirm the selection 18 10 The count 01 and aspirating volume will appear on the display The A pipetting direction and speed will flash to indicate that the pipette is ready for liquid pick up Press the START button to aspirate the samples H will appear to remind users to create an air gap between sample aspirations Ill 11 When the aspirations are completed will flash to indicate the pipette is ready for dispensing 12 After the sequential aspirating cycle the pipette will make two beeping sounds Pressing the START button will re activate the whole cycle again until users change the function mode Note 1 If the blow out setting is selected the pipette will automatically blow out the liquid during sequential aspiration mode 2 E1000 models do not include the Sequential Aspirating SA function because the air gap is not strong enough to support the weight of samples 3 Sequential Aspirating SA function will not perform Reverse Pipetting even it is selected in System SYS mode 10 2 SD Sequential Dispensing Mode The CappTronic performs repetitive dispenses of the selected volumes When the first aspiration is completed the pipette will sequentially dispense the aspiration volume 1 Press EXIT to enter function mode selection 2 Press Up Down repeatedl
14. months Thus users should establish a regular testing routine for their pipette having regard to the accuracy requirements of the application frequency of use number of operators using the pipette nature of the liquid dispensed and the acceptable maximum permissible errors established by the user ISO 8655 1 For maintenance please contact your CappTronic dealer or authorized service provider You may find useful additional information on our website www cappbrand com Note Using improper lubricants will deter or block the movement of the piston of the pipette and will cause loss of warranty 13 Troubleshooting To ensure the product quality the pipette is designed with a self diagnosis program This program will constantly monitor the accuracy of pipetting volume battery status and auto calibration function Error Err messages will appear on the display if the pipette fails to perform the attempted action properly In case any Error Err messages or faults occur please refer to the solutions in the following table to clear the error messages or faults If the following solutions are not able to resolve the problem please return your pipette to your CappTronic dealer or an Capp authorized service provider for repair Symptom Possible Cause Solution Err 01 Bad battery Charge the battery Bad power adaptor Replace the power adaptor Err 02 Auto calibration is not Press the working b
15. operating condition for each dispensing step 1 2 3 Press EXIT to enter the mode selection Press Up Down to make the MD symbol start flashing Press SET to confirm the selection The COUNT symbol will then appear 16 Use s Up Down to select the desired pipetting count Press D SET to confirm the selection of count The default pipetting volume will start flashing Use gt Up Down to select the desired pipetting volume Press D SET to confirm the pipetting volume The pipetting volume will show the total aspirating volume After the settings are completed firstly press the START button to aspirate the sample according to the pipetting volume Next continue to press the START button to dispense the samples according to the pipetting counts When the count becomes 0 press the button to aspirate the sample again By pressing the button the whole operation cycle will automatically repeat 9 MIX Mode After the settings are completed Mixing is performed automatically as long as the START button is held down or with just one touch of the START button the pipette also can finish the programming 1 2 Press EXIT to enter mode selection Press Up Down repeatedly until the MIX symbol starts flashing Press SET to confirm the selection The COUNT symbol will then appear Use Up Down to
16. rm is designed to remind the users that the tips have been ejected It helps the users avoid using the same tip to contaminate different reagents 1 The Tip Ejector Alarm symbol me should now be flashing 2 Press Up Down to select the preference mA On Off 3 Press D SET to confirm the selection and move to the Blow out setting 6 5 Blow out The automatic blow out function in different pipetting modes simulates the blow out function in manual pipettes To avoid the leftover final droplet in tips the blow out setting is recommended in all protocols The blow out setting will not be performed in Reverse Pipetting setting 1 The Blow out symbol should now be flashing 2 Press Up Down to select the preference On Off 3 If is selected pressing D SET will confirm the selection and move to the PROG setting If is selected pressing P SET will confirm the selection and move to the Reverse Pipetting setting Note It is important to withdraw the tip quickly from the dispensing sample vessel after dispense if Blow out is selected because the piston will retract a short distance for creating the blow out volume 6 6 Reverse Pipetting The automatic reverse pipetting in different pipetting modes is to reserve the final drop of sample The pipette will not perform the reverse pipetting in MIX or SE modes 1 The Reverse Pipetting
17. s or if other than original spare parts supplied by the manufacturer have been used Each pipette is tested and documented by the manufacturer before shipping Capp Quality Control System guarantees that the performance of the CappTronic pipette you have purchased is within its specifications 27 Your Notes Your Notes Your Notes Printed in Taiwan P N 401 apecap 29 2008 1 1
18. select the desired mixing count Press D SET to confirm the selection The default pipetting volume will start flashing Use Up Down to select the desired mixing volume Press SET to confirm the selection The total mixing volume and counts will be displayed Press the START button to start the mixing function After the completion of mixing pressing the START button will re activate the mixing process until the user changes the function mode 10 SE Sequential Aspirating Dispensing Mode SE mode includes SA Sequential Aspirating mode and SD Sequential Dispensing mode 10 1 SA Sequential Aspirating Mode The pipette performs repetitive aspirations of the selected volumes An air gap will be created automatically between two aspirations When the sequential aspiration is completed the next operation will dispense all of the aspiration volume together 1 2 3 4 5 6 Press EXIT to enter function mode selection Press Up Down repeatedly until the SE symbol starts flashing Press D SET to confirm the selection Use Up Down until the SA symbol starts flashing Press SET to confirm the selection Under the COUNT symbol 01 will appear Press s Up Down to set the volume of the first aspiration Press SET to confirm the selection Repeat steps 6 and 7 to set the sequential aspiration volumes The total aspirating volume inc
19. t 2 hours You can follow Figures 11 14 to disassemble the pipette Please DO NOT use excessive force to pull down the lower part before releasing the cylinder lock as shown in Figure 10 This action may break the connection mechanism of the lower part 20 Cylinder lock Warning Don t use force to pull the lower portion before release the cylinder lock Figure 9 Wrong operation of Lower Part disassembly Disassemble Lower Part Always remember to press button before you disassemble the lower part of the pipette Press the button Wait until you hear two beeps Loosen the lower part of the pipette by rotating the connecting nut in the clockwise direction as shown in Figure 11 Pull the lower part downward slowly until the cylinder lock appears as shown in Figure 12 Please place the pipette on a flat surface Push the cylinder lock downwards until you hear a click sound as shown in Figure 13 The lower part will come off easily and automatically as shown in Figure 14 21 Figure 10 Rotate the connecting nut Figure 11 Pull the lower part clockwise downward slowly until the cylinder lock appears Cylinder lock Figure 12 Push the cylinder lock Figure 13 The lower part will downward until you hear a click sound come off easily and automatically 22 11 2 Reassemble Lower Part AN Always remember to press button after you disassemble the lower part of the pipette Follow these steps to reassemble
20. utton Err 03 Inaccurate pipetting volume Press the 24 button Err 04 Step motor failure Photo couple failure Press the button Droplets left inside the tip Unsuitable tip Use high quality tips Leakage or volume too small Non uniform wetting of the plastic tip Rinse the tip Tip incorrectly attached Unsuitable tip Attach firmly Use high quality tips Fail to aspirate Low battery Charge the battery The lower part doesn t hook up correctly with motor shaft Reassemble the lower part one more time Foreign objects block the tip Use MIX mode fitting s channel and distilled water to wash away the foreign objects Piston blocks Lubricate piston Power on failure Bad battery contact Reinsert the battery Rusted battery contact Replace with new battery Dead battery Charge the battery Function Mode Tip Ejector can t bounce Adjust Tip setup failure back 25 Ejector position Appendix A Technical Specifications Automatic Pipetting AUTO Multiple Dispensing MD Mixing MIX Operation Mode Sequential Aspirating Dispensing SE System Setup SYS Memories 9 sets Aspirating Dispensing Speed 5 speeds Auto calibration Yes Power Engine High precision stepping motor Power Saving Yes after 10 minutes Power Adaptor 100 120 220 240 V AC
21. w OUt a orto t RE re rinnen 13 6 6 Reverse Pip ero 14 6 7 Setting PROG Programs seisis enno ieseni n etteren pios iassa 14 Te AUTO MOUE s ssssssrssseressesserensescesenssecesesesceseneescssssessessesessesessessesess 15 8 MD Multiple dispensing Mode eere erect eene eene 16 De MEX MOU M s 17 10 SE Sequential Aspirating Dispensing Mode 18 10 1 SA Sequential Aspirating Mode eee 18 10 2 SD Sequential Dispensing Mode seen 19 11 Sterilization cessessusssssorsnnsnsnnesssnssnnsonsnssnnsonsnnsnnsnnsnnssnnnnsonsnnsnnsnnsnnnnnn 20 11 1 Disassemble Lower Part essseeeeereee 21 11 2 Reassemble Lower Part screen eee cre en 23 12 Maintenance c sosssnssnssessnssnssonsnnsnnsnnsnnssnssnnsonsnnsnnsnnsnnssnssnnsonsnnssnsnnsnnnsnn 23 13 Troubleshooting erssorsoossonssnnssnsssnnennnensnssssssnssonssonsnnssonssonssnnsnnnsnnsenne 24 Appendix A Technical Specifications sorsosssessnssorsorsnnsonsnnsnnsnnennnnnsnnenene 26 Appendix B Tip Ejector Height Adjustment ceret 26 Appendix C Warranty sorsssssssnsssssnssorsossnssnnsonsnnsonsnnsnnsnnssnnsonsnnsonsnnssnsnnnnnne 27 1 Safety Precautions Congratulations to your new CappTronic Before using it for the first time please read this entire operating manual carefully To gu
22. y until SE symbol starts flashing 3 Press D SET to confirm the selection 19 4 Press Up Down repeatedly until Sd symbol starts flashing 5 Press D SET to confirm the selection 6 Under COUNT symbol 01 will appear Press S Up Down to set the volume of the first dispense 7 Press D SET to confirm the selection 8 Repeat steps 6 and 7 to set the sequential dispensing volumes The total dispensing volume cannot exceed maximum volume range 9 Press D SET to confirm the selection 10 The count 01 and the total aspirating volume will appear on the display The pipetting direction and speed will flash to indicate the pipette is ready for liquid pick up Press the START button to aspirate the samples When the aspirations are completed the dispensing count v and volume will appear and amp will flash to indicate that the pipette is ready for dispensing 11 After the sequential dispensing cycle the pipette will make two beeping sounds Pressing the START button will re activate the whole cycle until the user changes the function mode Note If the blow out setting is selected the pipette will automatically blow out the liquid during sequential aspiration mode 11 Sterilization Only the lower part of pipette can be steam autoclaved 121 C 1 bar 20 minutes The autoclaved parts must be allowed to dry completely at room temperature for at leas
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