Tissue Bath System - 720MO - User Manual
Contents
1. 1 1 PC data acquisition and analysis software I optional i 1 I l I 1 I 1 1 I I l 1 I l I i Interface front panel Pa ee S 1 USB connection ER Connection to oxygen supply E 3 AAA I 1 1 Om _ BNC Cables 1 p bo 1 Vacuum pump ACIDO a ae P optional a eee Suction bottle optional Figure 2 1 The complete Tissue Bath System 720MO 2 2 Setting up step by step This chapter contains a complete step by step description of how to set up a Tissue Bath System 720MO as illustrated in figure 2 1 above 1 Interface PC connection Data acquisition is possible either by connecting the Interface directly to a PC or through a PowerLab data acquisition and analysis system optional l Direct PC connection Connect the Interface to one of the COM ports on the PC using a serial cable cable not included Il PowerLab optional Connect the Interface to the PowerLab unit using BNC cables Connect Rec 1 on the Interface to Input 1 on the Power Lab Rec 2 to Input 2 etc Connect the PowerLab unit to one of the USB ports on the PC using the USB cable delivered with the PowerLab system Oxygen supply Conne2. Part 1 General requirements EN 61010 2 101 2003 Safety requirements for electrical equipment for measurement con trol and laboratory use Part 2 101 Particular requirements for in vitro diagnostic IVD medical equipment EN 61326 2 6 2005 Electrical equipment for measurement control and laboratory use EMC requirements Part 2 6 Particular requirements In vitro diag nostic IVD medical equipment With reference to regulations in the following directives 2006 95 39 EEC CERTIFICATE OF CONFORMITY 7 ABOUT THIS MANUAL This manual contains a complete list of procedures that describe how to install maintain and use the Tissue Bath System Chapter 1 provides an overview of the construction and basic features of the interface and the Tissue Bath unit Chapter 2 describes step by step instructions to set up a complete Tissue Bath System including accessories Chapter 3 is a complete manual for the 720MO interface This chapter describes in detail how to navigate the menus and how to use the special features of the Tissue Bath System 720MO Chapter 4 contains procedures describing general and daily maintenance of the Tissue Bath unit e g adjustment of supports weight calibration of the force transducers and cleaning instructions Appendix contain system specifications 8 TISSUE BATH SYSTEM MODEL 720MO USER MANUAL UNPACKING THE TISSUE BATH SYSTEM Take a few minutes to carefully inspect your new Tissue
3. 06 GJ G Place the calibration bridge on the myograph Be careful when placing the bridge The calibration arm must not touch the mounting pin When ready go to next step BACK NEXT STEP Step no Turn the heat Wait until the temperature is stable Temperature set point 37 0 C Probe temperature 36 8 C CHAMBER 1 CALIBRATION Step no OOUR oon Turn the heat on Wait until the temperature is stable Temperature set point 37 0 C Probe temperature 36 8 C BACK eo HEAT OFF NEXT STEP Step no og Make sure that the transducer 15 not subjected to any force When the relative force reading is stable go to next step Force Chamber 1 3261 NEXT STEP Step no JIM Carefully place the 2 g weight On the pan When the relative force reading is stable go to next step Force Chamber 1 3346 NEXT STEP CHAPTER 3 17 Step 6 is to verify that the calibration was performed correctly The Force Chamber 1 reading should be 9 81 0 1 mN If the Force Chamber 1 reading is off by more than 0 1 mN then remove the weight press BACK to return to Step 4 and repeat the calibration process If the Force Cham ber 1 reading is satisfactory then press NEXT STEP Calibrate the other chambers in the same manner 2 VALVE DELAY Pressing VALVE DELAY in the SETTINGS menu will allow the user to modify the time duration that the vacuum valves stay op
4. Bath System for damage which may have occurred during handling and shipping If you suspect any kind of damage please contact DMT immediately and the matter will be pursued as soon as possible If the packing material appears damaged please retain it until a possible claim has been settled We recommend that you store the packing material for any possible future transport of the Tissue Bath System In case you need to transport and the original packing material is unavailable please contact the DMT Sales Department for advice and packing instructions After unpacking your new Tissue Bath System use the following list to check that the system is complete e interface unit e 4chambers with mounted pin supports 200 um e 4chamber covers e 1external temperature probe e 1 power cord e 1calibration kit including bridge balance and 2 gram weight e plastic funnels e 1tube of high vacuum grease e 1 tube of grease for linear slides e 2 Allen keys e 1small screwdriver e 1 with user manuals for Tissue Bath Systems e 1 CD with the manual Procedures for investigation of small vessels using small vessel myograph by Professor J Mulvany Department of Pharmacology Aarhus University Denmark and the video Dissection and mounting of small vessels in wire myographs The shape of the AC plug varies by country be sure that the plug fits the outlets for your location UNPACKING THE TISSUE BATH SYSTEM
5. ane enc EE ic csi d Dub e n RR raa E REA edi 24 4 5 Tissue Bath System MalhtenaN Criar iaa 26 Appendix 1 System 28 NOTOG NR X 29 4 TISSUE BATH SYSTEM MODEL 720MO USER MANUAL SAFETY The Tissue Bath System 720MO has been designed for use only in teaching and research applications It is not intended for clinical or critical life care use and should never be used for these purposes or for the prevention diagnosis curing treatment or alleviation of disease injury or handicap e Do not open the unit the internal electronics pose a risk of electric shock e Do not use this apparatus near water e To reduce the risk of fire or electric shock do not expose this apparatus to rain or moisture Objects filled with liquids should not be placed on the system e Do not block any ventilation openings Install in accordance with the manufacturer s instructions e Do install near any heat sources such as radiators heat registers stoves or other items that produce heat e Only use attachments and accessories specified by the manufacturer e Unplug this system during lig
6. as possible without touching as illustrated in Figure 4 4 below One way to do this is to use the following technique Start with the calibration kit in place so that the tip of the calibration arm is not touch ing any part of the tissue holding pins Go to the main menu displaying the forces and zero the channel being calibrated so the force reads zero Slowly and gently slide the calibration kit forward toward the micrometer so that the calibration arm rests on the tissue holding pin creating a force reading on that channel Carefully slide the calibration kit back toward the transducer slowly until the force reads zero At this point as soon as the force reads zero the calibration arm will be properly placed for weight calibration 4 Goto the FORCE CALIBRATION sub menu of the SETTINGS menu on the Interface to begin the actual transducer calibration The process that is described above is reiterated in 6 steps once the FORCE CALIBRATION sub menu is initiated which is described in detail in FORCE CALIBRATION sub menu in 3 1 4 Settings menu on page 16 e Close up JOE eee AAA EE II The tip of the calibration arm is positioned beside the tissue holding pin Figure 4 4 Weight calibration kit shown in place on the chamber CHAPTER 4 23 4 3 Checking the force transducer The Tissue Bath force transducer is a strain gauge connected to a Wheatstone bridge The force transducers for each chamber are housed in a separate protect
7. can accommodate mounting supports for larger segments gt 500 um Because the mounting supports can be changed easily experiments can be performed with different vessels of varying internal diameter from 500 um up to 10 mm Continuous use and repeated greasing of the transducer arm holes will cause some misalignment of the mounting supports The mounting supports will need occasional adjustments Changing and adjustment of the supports is performed using the following step by step procedure NOTE THE TRANSDUCERS ARE FRAGILE AND SENSITIVE TO MECHANICAL STRAIN BE VERY CAREFUL WHEN CHANGING OR AD JUSTING THE MOUNTING SUPPORTS Changing the supports Figure 4 1 1 Use the micrometer to separate the supports as far apart as possible 2 Use the small screwdriver provided to gently loosen screw D on the support attached on the transducer side Screw D is the screw on the support closest to the transducer 3 Gently pull the support away from the transducer pin 4 Loosen screw B on the micrometer side with the appropriate Allen key 5 Pull the pin support away NOTE number the supports with the number of the chamber they were removed from using some kind of permanent marker Store the supports in the provided plastic case Numbering the supports will save time when the supports are changed again limiting the amount of adjustments needed after each change Figure 4 1 Tissue Bath chamber screws for changing supports and adjustment of th
8. into the chamber as illustrated in Figure 2 2 below A chamber can then be emptied by pressing the corresponding numbered button Pressing the all button will empty all the chambers at the same time see valve buttons in Figure 1 1 Interface front panel with 720MO chambers on page 10 NOTE WHEN DRAINING THE CHAMBERS USING THE AUTOMATIC VACUUM FUNCTION PRESS THE APPROPRIATE BUTTON FOR AN ADDITIONAL 3 5 SECONDS AFTER THE INITIAL EMPTYING THIS WILL HELP DRAIN RESIDUAL BUFFER AND SOLUTION RETAINED IN THE TUBING AND VALVES 4 Chamber covers The chamber covers will help maintain the temperature and other buffer conditions gas tension pH fairly constantly Holes in the chamber covers serve different purposes and they are illustrated in Figure 2 3 below The slots allow the covers to be placed over the chamber around the support arms and gas vacuum tubes pee e Suction Figure 2 2 Oxygen supply and suction connection e Funnel o e For drug application y b Si e Temperature probe Figure 2 3 Chamber cover Figure 2 4 Chamber cover with built in electrodes optional item 2 3 The first weight calibration Prior to shipment the Tissue Bath System 20MO has gone through two days of continuous testing including a final force trans ducer calibration However DMT recommends that a new force transducer calibration is performed before using the Tissue Bath System for the fir
9. though the chamber is plugged in at the rear of the interface the force transducer is broken and needs to be replaced In addition if the force reading s appear yellow in color cannot be reset to zero AND the transducer cannot be recalibrated the force transducer is broken and needs to be replaced If any other problems related to the force transducer are encountered please contact DMT for advice or further instructions 4 4 Force transducer replacement If the force transducer breaks and needs to be replaced follow this step by step replacement procedure carefully 1 Remove the pin from the transducer pin coming out of the transducer house 2 Disconnect the Tissue Bath chamber from the interface 3 Turn the Tissue Bath chamber upside down and remove the transducer housing by loosening the two screws A B as illustrated in Figure 4 6 The 2 screws that secure the transducer house to the chamber on next page 4 The replacement transducer will be shipped with the new transducer inside a new transducer house 5 Place a small amount of vacuum grease clear or whitish grease around the bottom of the transducer housing to seal the transducer housing when put back in place see Figure 4 7 The transducer in the transducer housing and close up of transducer pin inside the transducer on the next page 6 Carefully realign the transducer housing with the new transducer on the Tissue Bath chamber and reinsert the Allen screws through t
10. 9 CHAPTER 1 SYSTEM OVERVIEW 1 1 Interface front panel Interface display e Heat indicator Power indicator DMT ero Valve buttons Figure 1 1 Interface front panel with 720MO chambers 1 2 Interface rear panel 4 transducer connections Gas regulator needle valve 9 Gas input TRANS 4 o OXYGEN i POWER USB ES 2av oc L O Power connector A ON OFF switch Vacuum input SY RS 232 Port for serial connection to PC o Temperature probe o REC 4 REC 3 REC L REC 1 ak w Y W USB output Figure 1 2 Interface rear panel 10 TISSUE BATH SYSTEM MODEL 720M0 USER MANUAL 1 3 The Tissue Bath unit Connection to interface Micrometer Figure 1 3 Tissue Bath unit Force transducer pin E 4 Pin support connected to ee ies e Pin support connected to micrometer force transducer Figure 1 4 Close up of Tissue Bath unit CHAPTER 1 11 CHAPTER 2 SETTING UP 2 1 The complete Tissue Bath System 720MO PowerLab Data Acquisition System DMT CS200 Pulse Train Stimulator optional optional i Es t9 1 unie ciui qe curn M 0 2 I 1 EA l I ot IM ROA i I l 1 I I 1 L 1 I 1 I
11. A E A 9 6 Certificate nano ll Rr ERR DDR CREE UE C E E DR REIR VIN Ec TRE D RD RR V ER 7 A An aa 8 Unpacking the Tissue Bath System c ssccssssecsensecenssseeesseeenssseenassenesseennaseeeaseeenaseeensseoeesseoneaseosssenenseeonsseeosssenensseensaseneassenensseonaas 9 Chapter 1 System OVE VOW aaa 10 T aC COMIC PUE UU PP 10 UN METAS cH gt aran anar m 10 LO i is EI rr A 11 Chapter 2 SO W MP a 12 2 1 The complete Tissue Bath System 720M0 risa akku uxdacVadud S nta Ga ra reca e adul 12 2 2 SCHNE STED nai aO DO Cebu Vm unti Savi cua bu El ad fanis RE dan 12 SNA EA 13 Chapter 3 The interface MENUS mi ER A RE HER ER ADHERE BA 14 MIN quA so menus aaa aria m ii O O a 14 Chapter 4 The Tissue A MM EUER 21 4 1 Changing and adjusting the MOUNTING SUD DOTS aries caladas 21 2 Calibration of the force Trai dB BI VER ERI YS E 23 Z3 the Translated 24 4 4 Force transducer replacement wisi
12. DANISH MYO TECHNOL Ost TISSUE BATH SYSTEM MODEL 720MO 2 TISSUE BATH SYSTEM MODEL 720MO USER MANUAL TISSUE BATH SYSTEM MODEL 720MO TRADEMARKS Pentium is a registered trademark of the Intel Corporation Windows Windows 95 Windows 98 Windows ME Windows NT Windows 2000 Windows XP Windows Vista and Windows 7 are registered trademarks of Microsoft Corporation All other trademarks are the properties of their respective owners Danish Myo Technology A S reserves the right to alter specifications as required This document was as far as possible accurate at the time of printing Changes may have been made to the software and hardware it describes since then New information may be supplied separately This documentation is provided with the DMT Tissue Bath System Model 720MO All rights reserved No part of this manual may be reproduced or transmitted in any form or by any means without the written permission of Danish Myo Technology A S Every attempt is made to ensure accurate information misprints construction and specification changes can occur Danish Myo Technology A S reserves the right to alter change content as required and without any notice Copyright O Danish Myo Technology A S DANISA MYO TECHNOL OGS Trademarks 3 CONTENTS o e e 3 m EN E N N EENE E EE E E 5 E E E A E E 6 POT OV ANS E E EE E E E E E E neous A E E E N E
13. ENT CALIBRATION DELAY OUTPUT RANGE INTERFACE SETTINGS FORCE CALIBRATION x Force calibration chamber 1 Force calibration chamber 2 SELECT Force calibration chamber 3 SELECT Force calibration chamber 4 SELECT FORCE CALIBRATION X Force calibration chamber 1 SELECT Force calibration chamber 2 SELECT Force calibration chamber 3 SELECT Force calibration chamber 4 SELECT 29550 Follow the Weight calibration procedure in the User Manual Prepare the mounting pins and chamber for calibration When ready go to next step Step 2 involves setting up the calibration kit appropriately for the actual weight calibration Verify that the tissue holding pin does not touch the cali bration arm as instructed The calibration arm should be as close as pos sible to the tissue holding pin support without touching in order to get the most accurate calibration Press NEXT STEP when the calibration kit is in position Step 3 initiates the heating process for the chambers In order for the cali bration to be accurate the transducers must be heated to the intended ex perimental temperature to compensate for heat induced expansion of parts in the transducer Otherwise inaccurate readings and transducer drift may occur To start heating press HEAT ON Covering the chambers with the cham ber covers will expedite the chamber heating Place the temperature probe into the chamber for the first calibration to
14. SELECT Once the desired setting has been chosen pressing ENTER will lock the selection and be stored in memory Range Chamber 1 200 mN SELECT ENTER Pressing the white X in the red box will exit that menu and take you auto matically to the Actual Force Display 3 1 1 Power up screen DMT720 MYOGRAPH After turning on the 720M0 interface an Introduction screen appears Multi Myograph System is auto calibrating the A D converters while this screen is dis Model DMT720 played Software Revision 03 00 09 Date Nov 27 2009 14 TISSUE BATH SYSTEM MODEL 720M0 USER MANUAL After a few seconds the ACTUAL FORCE display will appear ACTUAL FORCE Force chamber 1 Force chamber 2 Force chamber 3 Force chamber 4 Probe temperature E At any given time if the force applied on any channel is out of range the force reading for the overloaded channel will turn yellow as a warning ACTUAL FORCE Force chamber 1 234 36 mN Force chamber 2 0 26 mN Force chamber 3 1 06 mN Force chamber 4 0 76 mN Probe temperature 37 0 C C JC Three menus are accessible from the default ACTUAL FORCE screen or aca roncE B display These menus are Zero Heat and Settings ACTUAL FORCE E Force chamber 1 Force chamber 2 Force chamber 3 Force chamber 4 Probe temperature 3 1 2 Zero menu SET FORCE TO ZERO This menu is used to zero the output of the transducers When using a d
15. al temperature probe Therefore the user can adjust the temperature of each chamber individually to fine tune the temperature setting so that Chamber 4 SELECT EXACT temperatures can be achieved for any particular chamber This is referred to as a temperature offset TEMP OFFSET ON CHAMBER Chamber 1 Chamber 3 The SELECT and functions are the same in this menu as previ ously described for the VALVE DELAY menu Pressing ENTER will store the numbers in memory for future experiments Chamber 1 SELECT Chamber 2 SELECT Chamber 3 SELECT Chamber 4 SELECT Il FACTORY DIAGNOSTICS Entering FACTORY DIAGNOSTICS will display the LOGIN CODE TO DIAG NOSTICS window This window is for trained technicians and used for diagnostics and troubleshooting purposes The general user will not have access to this window Entering the proper 5 digit pin number however will allow the trained technician access to Diagnostics panels that will provide information during a malfunction or mechanisms to 5 2 o change other settings controlled by the onboard computer Code init value 51761 C JCOC OC C Type login code to get acces 20 TISSUE BATH SYSTEM MODEL 720MO USER MANUAL CHAPTER 4 THE TISSUE BATH UNIT This chapter contains a complete explanation of how to adjust calibrate and maintain the Tissue Bath System 4 1 Changing and adjusting the mounting supports Each chamber
16. ata acquisition program like LabChart by AD Instruments 0 using this feature Force chamber 1 2 36 mN SELECT will reset the baseline of the chart traces without affecting the calibrations or physically changing any pre load tensions placed on the mounted ves sels The channels can be changed individually by pressing SELECT or all Force chamber 3 1 06 mN SELECT at once by pressing ALL Pressing ENTER will execute the zero function and return the user to the ACTUAL FORCE display Force chamber 2 0 26 mN SELECT Force chamber 4 0 76 mN SELECT 3 1 3 Heat menu The heating unit and temperature are controlled from this menu To turn the heat on or change the preset temperature for the system access the temperature control menu Pressing the HEAT key will enter the menu and allow the user to change the default system temperature as well as turn the heat on or off Pressing DEFAULT will automatically reset the temperature Probe temperature 36 6 C setpoint to 37 C Manually change the temperature by pressing the up or down arrows Temperature setpoint 37 0 C HEAT CHAPTER 3 15 To turn the heat on touch ON and the ON icon will turn green The sys tem will heat to the designated temperature setpoint A little red light to the right of the touch screen will turn on when the heat is turned on Pressing the white X in the red box will send the user back to the ACTUAL FORCE dis
17. ct the gas supply 95 O 5 CO or 21 0 5 CO balance with tubing running from the gas supply to the gas inlet on the back of the Interface Oxygen is supplied to the chambers by tubing attached to the black stainless steel oxygen pipe The oxygen and vacuum tubing need to be inserted into the chamber in order to aerate the heated buffer and emptying the chambers respectively Needle valves on the back of the interface can be adjusted to regulate the amount of bubbling that occurs Turning the regulator clockwise increases the bubbling while turning it counter clockwise decreases the bub bling Each regulator has a lock device attached that can be used when the desired bubbling is achieved see Figure 2 2 Oxygen supply and suction connection on page 13 NOTE THE NEEDLE VALVES NEED TO BE GREASED USING THE GREASE FOR THE LINEAR SLIDES AND TURNED AT REGULAR IN TERVALS TO PREVENT THEM FROM STICKING OR PERMANENTLY LOCKING 12 TISSUE BATH SYSTEM MODEL 720MO USER MANUAL 3 Vacuum connection The system has a built in drainage system with separate valves that allows each chamber to be emptied individually After connecting the vacuum source to the vacuum pipe at the back of the Interface the vacuum pipes need to be inserted into the chambers in order for this feature to work properly The pipes are inserted into the chamber by gently pulling up on the curved part of the pipe turning it 90 counter clockwise and gently lowering it
18. d water Any residual acid will cause corrosion of the stainless steel pins IMPORTANT NOTES BE VERY CAREFUL USING HCL OR HNO BECAUSE THESE ACIDS MAY CAUSE EXTREME DAMAGE TO THE STAINLESS STEEL CHAMBERS AND SUPPORTS DO NOT USE BLEACH TO CLEAN THE CHAMBERS REPEATED USE OF CHLORINATED SOLUTIONS SUCH AS BLEACH AND HCL WILL CAUSE DAMAGE TO THE STAINLESS STEEL PARTS OF YOUR TISSUE BATH SYSTEM AVOID USING THEM IF AT ALL POSSIBLE AFTER CLEANING ALWAYS CHECK THAT THE GREASE AROUND THE TRANSDUCER PIN IS SUFFICIENT TO KEEP THE BUFFER AND WATER FROM ENTERING THE TRANSDUCER HOUSING If red or brown discolorations appear on the chamber sides or on the supports the following cleaning procedure will work in most cases f Incubate the Tissue Bath chamber and supports for 30 minutes with 2mM T 1210 Tetrakis 2 pyridylmethyl ethylenediamine solution dissolved in double distilled water 8 Use a cotton tip applicator to mechanically clean all the affected surfaces during the last 15 minutes of the incubation period 9 Wash the Tissue Bath chamber and supports several times with double distilled water 10 Incubate the Tissue Bath chamber with 96 ethanol for 10 minutes while continuing the mechanical cleaning with a cotton tip applicator 11 Remove the ethanol solution and wash a few times with double distilled water Incubate the Tissue Bath chamber and Supports with an 8 acetic acid solution for 10 minutes and continue the mechanical cleaning w
19. e pins CHAPTER4 21 Fine adjusting the pins for larger vessels Figure 4 2 and 4 3 1 Loosen screw A to move the micrometer side arm holder sideways 2 Loosen screw B to move the micrometer side pin toward or away from the transducer 3 Loosen screw Cto align the transducer side tissue holding pin horizontally 4 Loosen screws D and E to align the heights of the tissue holding pins vertically A B E DC Figure 4 2 Fine adjustment of the pins in the Tissue Bath chamber Tissue holding pins from top view Tissue holding pins from side view E v X Z Figure 4 3 Illustrations of properly aligned tissue holding pins depicted on the far left and incorrectly aligned pins depicted in the middle and far right 22 TISSUE BATH SYSTEM MODEL 720M0 USER MANUAL 4 2 Calibration of the force transducer As a part of the general maintenance of the Tissue Bath System DMT recommends that the Tissue Bath System is weight cali brated at least once a month The Tissue Bath System should also be weight calibrated every time the interface has been moved Although lab benches are all supposedly perfectly horizontal small differences in lab bench pitch can affect the calibration of the system The Tissue Bath System also should be calibrated if the system has been idle for longer than a month A step by step procedure is included in the FORCE CALIBRATION sub menu in 3 1 4 Se
20. en for washes Factory default is set at 1 second but 1 second is not enough time to completely empty a chamber with even as small a volume as 5 ml Pressing SELECT next to any given channel will cause the line selected to turn blue The up and down arrow keys can then be used to modify the length of time the vacuum valves stay open after the valves have been acti vated with the valve buttons on the front of the interface Pressing ALL will cause all the lines to turn blue meaning all chambers will be modified at the same time Again the up and down arrow keys can be used to modify the length of time the vacuum valves stay open Pressing ENTER after modifying the value s for valve delay will lock in the settings They will be retained in memory and used when the system is next turned on 18 TISSUE BATH SYSTEM MODEL 720MO USER MANUAL CHAMBER 1 CALIBRATION sev OOOO The transducer is now calibrated Force read out should be 9 81 mN 0 1 mN If OK go to next step Otherwise repeat the calibration Force Chamber 1 9 81 mN EMPTY VALVES DELAY Chamber 1 Chamber 2 Chamber 3 Chamber 4 EMPTY VALVES DELAY Chamber 1 Chamber 2 Chamber 3 Chamber 4 EMPTY VALVES DELAY Chamber 1 Chamber 2 Chamber 3 Chamber 4 NEXT STEP 3 FORCE RECORDING OUTPUT The FORCE RECORDING OUTPUT or FORCE REC OUT sub menu deter mines the upper limit for force sent from the BNC analo
21. gue outputs This will only affect the data collected from the interface by a data acquisition system such as an AD Instruments PowerLab The factory default setting for FORCE REC OUT is 20 mN meaning that if the force of the mounted vessel exceeds 20 mN the force recorded by the data acquisition system will not record more than 20 mN and will appear as a flat line trace at 20 mN even though the force readings on the interface may exceed 20 mN Therefore change the FORCE REC OUT settings to an appropriate setting to capture the maximum response from the vessel of interest This value should not exceed the settings for the transducer range which is defined by the sub menu MEASUREMENT RANGE and is explained in the next section The SELECT and ALL functions are the same in this menu as previously described for the VALVE DELAY menu Pressing ENTER will store the numbers in memory for future experiments NOTE ANYTIME FORCE REC OUT IS CHANGED A NEW WEIGHT CALIBRATION ON THE TRANSDUCERS SHOULD BE PERFORMED ENTERING THE NEW VOLTAGE VALUES INTO THE DATA ACQUISITION SYSTEM BEING USED 4 MEASUREMENT RANGE The MEASUREMENT RANGE sub menu in SETTINGS determines the maxi mum force capacity of the transducer The factory setting is 200 mN but the transducer capacity can be changed to 400 mN 800 mN or a maximum of 1600 mN of force detection depending on the size of the vessel used The SELECT and ALL funct
22. gure 4 9 The areas where linear slide grease may be applied for smooth micropositioner movement 26 TISSUE BATH SYSTEM MODEL 720MO USER MANUAL Cleaning the Tissue Bath DMT strongly recommends that the Tissue Bath chambers and surrounding areas are cleaned after each experiment At the end of each experiment use the following procedure to clean the Tissue Bath chambers and supports 1 Fill the Tissue Bath chamber to the edge with an 8 acetic acid solution and allow it to work for a few minutes to dissolve calcium deposits and other salt build up Use a cotton tipped applicator to mechanically clean all chamber surfaces 2 Remove the acetic acid and wash the Tissue Bath chamber and supports several times with double distilled water 3 If any kind of hydrophobic reagents have been used which might be difficult to remove using steps 1 and 2 then try incubating the chamber and supports with 96 ethanol or a weak detergent solution i e 0 1 triton 100 4 To remove more resistant or toxic chemicals incubate the Tissue Bath chamber and supports with HCI for up to 1 hour In exceptional cases incubate the chamber and supports with no stronger than a 3M HNO solution for about 15 minutes 5 Wash the Tissue Bath chamber and supports several times with double distilled water If acids such as 1M and HNO are used to clean the chambers make sure ALL surfaces are thoroughly dried after copious washes with double distille
23. he bottom of the Tissue Bath chamber f Tighten the screws and place some vacuum grease around the transducer pin that protrudes from the transducer housing see Figure 4 8 Close up of transducer pin from outside on the next page Make sure that the hole is completely sealed to prevent buffer solution or water from entering the transducer housing and damaging the new force transducer 24 TISSUE BATH SYSTEM MODEL 720MO USER MANUAL Figure 4 6 The two screws that secure the transducer house to the chamber Figure 4 7 Inside the transducer housing and close up of transducer pin The orange arrows and the dashed frame indicate the place that the vacuum grease needs to be applied to prevent water and buffer from damaging the transducer Figure 4 8 Close up of transducer pin from outside The arrow indicate the place that the grease needs to be applied to prevent water and buffer from damaging the transducer IMPORTANT NOTE CALIBRATE THE NEW FORCE TRANSDUCER BEFORE PERFORMING A NEW EXPERIMENT AS DESCRIBED IN CHAPTER THE INTERFACE MENUS ON PAGE 14 AND 4 2 CALIBRATION OF THE FORCE TRANSDUCER ON PAGE 23 CHAPTER 4 25 4 5 Tissue Bath System Maintenance The Tissue Bath System 720MO is a very delicate and sophisticated piece of research equipment DMT recommends that the following sections are read carefully and that the instructions are followed at all times Tissue Bath chamber tubing To prevent the t
24. htning storms or when unused for long periods of time advised that different operating voltages require the use of different types of line cord and attachment plugs Check the voltage in your area and use the correct type See the table below Voltage Line plug according to standard 110 125 V UL81 and CSA C22 2 No 42 220 230 230 V CEE 7 CEE 7 page VII SR section 107 2 D1 IEC 83 page C4 VII SR section 107 2 CEE 7 page VII SR section 107 2 D1 IEC 83 page C4 83 page C4 240 V SN 1363 of 1984 Specification for 13A fused plugs and switched and unswitched socket outlets NOTE PROTECT THE POWER CORD FROM BEING WALKED ON OR PINCHED PARTICULARLY AT POWER PLUGS AND THE POINT WHERE THEY CONNECT TO THE APPARATUS REFER ALL SERVICING TO QUALIFIED SERVICE PERSONNEL SERVICING IS REQUIRED WHEN THE APPARATUS HAS BEEN DAMAGED IN ANY WAY SUCH AS THE POWER SUPPLY CORD OR PLUG IS DAMAGED LIQUID HAS SPILLED ONTO OR OBJECTS HAVE FALLEN INTO THE APPARATUS THE APPARATUS HAS BEEN EXPOSED TO RAIN OR MOISTURE DOES NOT OPERATE NORMALLY OR HAS BEEN DROPPED SAFETY 5 EMC EMI This equipment has been tested and found to comply with the limits for a Class B Digital device pursuant to part 15 of the FCC rules These limits are designed to provide reasonable protection against harmful interference in residential installations This equipment generates uses and can radiate radio frequency energy and if not installed and used in acco
25. ions are the same in this menu as previously described for the VALVE DELAY menu Pressing ENTER will store the numbers in memory for future experiments FORCE REC OUTPUT RANGE Range Range Range Range Force1 Rec Range Force2 Rec Range Force3 Rec Range Force4 Rec Range 200 mN 1100 mN 20 mN 1200 mN 200 mN 1100 mN 20 mN 1200 mN MEASUREMENT RANGE Range Chamber 1 Range Chamber 1 Range Chamber 1 Range Chamber 1 200 mN 800 mN 400 mN 200 mN MEASUREMENT RANGE Range Chamber 1 Range Chamber 1 Range Chamber 1 Range Chamber 1 200 mN 800 mN 400 mN 200 mN SELECT SELECT SELECT SELECT SELECT SELECT SELECT SELECT SELECT SELECT SELECT SELECT SELECT SELECT SELECT SELECT CHAPTER 3 19 5 INTERFACE SETTINGS INTERFACE SETTINGS The INTERFACE SETTINGS sub menu in SETTINGS has an additional 2 sub menus These 2 additional sub menus are UE DIFFERENCE TEMPERATURE DIFFERENCE aa Il FACTORY DIAGNOSTICS DIAGNOSTICS l TEMPERATURE DIFFERENCE The TEMPERATURE DIFFERENCE function allows the user to finetune TEMP OFFSET ON CHAMBER the temperature setpoint of the system Although the temperature set point for the system can be set in the HEAT MENU the actual tempera ture for the system may not heat to the exact defined setpoint The Chamber 2 SELECT exact temperature in the bath can be measured using the extern
26. ith a swab stick 12 Wash the Tissue Bath chamber and supports several times with double distilled water 13 Dry the surfaces using absorbent paper or cotton tip applicators IMPORTANT NOTES IN EXCEPTIONAL CASES THE SUPPORTS MAY NEED TO BE REMOVED FROM THE TISSUE BATH CHAMBER AND CLEANED INDIVIDUALLY TO ASSURE PROPER CLEANING OF ALL SURFACES NEVER SOAK THE SUPPORTS IN ANYTHING STRONGER THAN 8 ACETIC ACID FOR EXTENDED PERIODS OF TIME I E SEVERAL HOURS OR OVERNIGHT CHAPTER4 27 APPENDIX 1 SYSTEM SPECIFICATIONS Technical specifications Tissue size Chamber Chamber material Chamber volume Chamber suction Chamber cover Chamber gassing Force range Force resolution Micropositioners Weight calibration Heating Temp range Temp resolution Temp probe Output reading Analogue output Voltage Ambient temp 500 um up to 10 mm as rings Four individual chambers Acid resistant stainless steel Max 8 ml Manual or automatic time controlled user defined Supplied with connections for gassing Individually controlled per chamber by needle valves User selectable at 200 400 800 1600 mN 0 1 mN Manually operated Semi automatic Built into chamber independent of superfusion Ambient temp 45 C 0 1 C External Force mN Independently filtered 4 channel output at 2 5V full scale 100 to 240 VAC auto 50 60 Hz via external power supply 15 30 C Optional accessories Automatic B
27. ive compartment Figure 4 5 below While the protective cover offers some mechanical protec tion for the force transducers they are still very vulnerable to applied forces exceeding 1 Newton 100 grams or fluid running into the transducer compartment due to insufficient greasing of the transducer pinhole Transducer house Figure 4 5 Illustration of the proper transducer house If the force readings on the Interface appear unstable or noisy then first check that the chambers are connected properly to the Interface and that the chambers are plugged all the way into the interface If the force reading s are still unstable or noisy then perform a new calibration of the force transducer as described in Chapter 3 The interface menus on page 14 and 4 2 Calibration of the force transducer on page 23 During the new calibration monitor the relative force reading values in the FORCE CALIBRATION sub menu on the interface Steps 4 and 5 of the calibration procedure The normal operating values for the force transducer during calibration should be between 3000 and 3500 e fthe value is O a single digit or a three digit number the force transducer is broken and needs to be replaced e the value is less than 2000 or greater than 4500 the force transducer has been broken and needs be shipped to DMT for further test and a temperature compensation e Ifthe message OFF is displayed on the main page of the Interface even
28. monitor when the chamber has reached the target temperature Heating will take about 20 to 30 minutes for the chambers and transducers to come to 37 C with the chamber cov ers in place Once the chamber s are heated and have reached the target temperature press NEXT STEP Step 4 is the first step in the actual weight calibration process A 4 digit number will be displayed in blue at the bottom of the screen If nothing has been disturbed during the heating process the zero O gram or 0 00 mN calibration should be stable after 20 30 sec indicated by the 4 digit number and NEXT STEP can be pressed at this time Normally the read ing will be stable after 20 30 sec If the 4 digit number is not stable then wait until the number has stopped fluctuating before pressing NEXT STEP Step 5 is the 2 gram weight calibration At this step place the 2 gram weight in the pan closest to the transducer so as to simulate a vessel pulling on the tissue holding pin closest to the transducer Remember a 2 gram weight in a 90 vector is cut in half and the transducer will only detect 1 gram or 9 81 mN of force The weight placement should cause a positive increase in the 4 digit number Wait at least 20 30 seconds for the applied force to stabilize Normally the reading will be stable after 20 30 sec If the 4 digit number is not stable then wait until the number has stopped fluctuating before pressing NEXT STEP Step no C 63 6
29. play 3 1 4 Settings menu The Settings Menu contains several sub menus that can be accessed to change functional aspects of the interface These sub menus include 1 FORCE CALIBRATION 2 VALVE DELAY 3 FORCE REC OUTPUT 4 MEASUREMENT RANGE 5 INTERFACE SETTINGS 1 FORCE CALIBRATION NOTE EVERYTIME A FORCE CALIBRATION IS PERFORMED THE MEAS UREMENT RANGE IS SET TO DEFAULT 200 mN SET MEASUREMENT RANGE AFTER THE FORCE CALIBRATION Entering the FORCE CALIBRATION sub menu begins the transducer calibra tion procedure Begin the calibration procedure by pressing FORCE CALI BRATION to enter the sub menu The sub menu will list all 4 chambers for calibration To begin the calibration press SELECT for the chamber you wish to cali brate The text for the chamber to be calibrated will turn blue Pressing EN TER will start the 6 step procedure for calibrating the force transducer on the desired chamber The calibration procedure is listed in 6 individual steps and needs to be per formed for each channel or transducer when calibrating the system Step 1 involves setting up the chamber for calibration Make sure the cham ber contains the tissue holding pin Fill the chamber with double distilled water Press NEXT STEP 16 TISSUE BATH SYSTEM MODEL 720MO USER MANUAL SET CHAMBER TEMPERATURE Temperature setpoint 37 0 C Probe temperature 36 6 C HEAT SETTINGS FORCE VALVE FORCE REC MEASUREM
30. rdance with the instructions may cause harmful interference to radio communications However there is no guarantee that interference will not occur in a particular installation If this equipment does cause harmful interference to radio or television reception which can be determined by monitoring the interference while turning the equipment off and on the user is encouraged to correct the interference by one or more of the following measures e Reorient or relocate the receiving antenna e Increase the separation between the equipment and receiver e Connect the equipment into an outlet on a circuit different to that which the receiver is connected to e Consult the dealer or an experienced radio TV technician for help APPROVALS Complies with the EMC standards 89 336 EEC 61326 2 6 2005 EN 61000 3 2 Certified with the safety standards Directive 2006 95 EC EN 61010 1 2001 EN 61010 1 Corr 1 2003 EN 61010 1 Corr 1 2003 EN 61010 2 101 2003 6 TISSUE BATH SYSTEM MODEL 720MO USER MANUAL CERTIFICATE OF CONFORMITY DMT A S Skejbyparken 152 8200 Aarhus N Denmark hereby declares its responsibility that the following product Tissue Bath System Model 720MO is covered by this certificate and marked with CE label conforms with the following standards EN 61010 1 2001 EN 61010 1 Corr 1 2003 EN 61010 1 Corr 1 2003 Safety requirements for electrical equipment for measurement con trol and laboratory use
31. st time The force transducer calibration procedure is described in detail in the FORCE CALIBRATION sub menu under SETTINGS as explained in Chapter 3 on the next page CHAPTER2 13 CHAPTER 3 THE INTERFACE MENUS Chapter 3 is a complete manual for the 720MO interface This chapter contains a detailed description of how to navigate the touch screen menus and how to use the special features of the 720MO 3 1 Interface menus Navigation Menus on the 720M0 interface are all accessible by a touch screen To SET FORCE ZERO access a menu simply touch the screen to access a menu When a set ting needs to be changed the setting can be changed by pressing the SE Force chamber 1 2 36 mN Sal LECT icon on the touch screen corresponding to the desired channel to be changed Force chamber 2 0 26 mN SELECT Force chamber 3 1 06 mN SELECT SELECT Force chamber 4 0 76 mN SELECT The line to be modified will turn blue indicating that the interface is waiting SET FORCE TO ZERO for input When ALL is chosen all lines corresponding to all 4 channels will turn blue Force chamber 1 2 36 mN SELECT Force chamber 2 0 26 mN SELECT ALL Force chamber 3 1 06 mN SELECT Force chamber 4 0 76 mN SELECT Changing the numeric value for the chosen parameter can be done by MEASUREMENT RANGE touching the up or down arrow keys VW Range Chamber 1 200 mN SELECT Range Chamber 1 800 mN SELECT Range Chamber 1 400 mN
32. ttings menu on page 16 Force transducer calibration procedure Figure 4 4 NOTE EVERYTIME A FORCE CALIBRATION IS PERFORMED THE MEASUREMENT RANGE IS SET TO DEFAULT 200 mN SET MEASUREMENT RANGE AFTER THE FORCE CALIBRATION This section contains step by step instructions to calibrate the force transducer and should be used in conjunction with the steps described in FORCE CALIBRATION sub menu in 3 1 4 Settings menu on page 16 1 Move the tissue holding pins apart Fill the chamber with distilled water or buffer Use the same volume that will be used during the experiments 2 Set up the calibration kit bridge and balance on one of the Tissue Bath chambers as illustrated in Figure 4 4 below Also place the weight on one of the chambers Turn the heat on as discussed in 3 1 3 Heat menu on page 15 The system takes about 20 to minutes to reach 37 C Lower temperatures take less time and higher temperatures take more time to reach Make sure adequate time is allowed so that calibration can be performed at the temperature at which the experi ments will be performed Placing the calibration kit and weight on the chamber allows them to warm up to the experimental target temperature There is no need to bubble the chambers while waiting for the system to heat up 3 When the system reaches target temperature adjust the calibration kit so that the tip of the calibration arm is as close to the tissue holding pin on the transducer side
33. ubing from becoming blocked with buffer salt deposits after an experiment remove the chamber cover from the Tissue Bath chamber and turn on the vacuum and press the vacuum valve for about 10 seconds by holding down the valve button s Turn off the vacuum and gas supply Remove any water or buffer remaining in the chamber or on the tubing using absorbent paper Force transducer The force transducer is the most delicate and fragile component of the Tissue Bath System Extreme care must be used when handling or touching the force transducers As a part of daily maintenance inspect the grease around the transducer pin extending from the transducer housing pinhole before starting any experiment Insufficient grease in this area will allow buffer and water to enter the transducer housing and cause damage to the force transducer IMPORTANT NOTES DMT RECOMMENDS THAT THE HIGH VACUUM GREASE SEALING THE TRANSDUCER PINHOLE IS CHECKED AND SEALED AT LEAST ONCE A WEEK ESPECIALLY IF THE TISSUE BATH IS USED FREQUENTLY DMT TAKES NO RESPONSIBILITY FOR THE USE OF ANY OTHER KINDS OF HIGH VACUUM GREASE OTHER THAN THE ONE AVAILABLE FROM DMT DMT TAKES NO RESPONSIBILITY FOR ANY KIND OF DAMAGE TO THE FORCE TRANSDUCERS Linear slides Check the linear slides under the black covers for grease at least once a week In case of insufficient lubrication grease the slides with the Grease for Linear Slides included with your system See figure 4 9 below Fi
34. uffer Filler System 625FS Chamber cover for field stimulation Combined pulse amp train generator CS200 28 TISSUE BATH SYSTEM MODEL 720MO USER MANUAL NOTES NOTES 29 DMT A S Skejby Science Center Skejbyparken 152 DK 8200 Aarhus N Denmark Tel 45 87 41 11 00 Fax 45 87 41 11 01 www dmt dk sales dmt dk support dmt dk DMT Asia Ltd Rm 2402B Great Eagle Centre 23 Harbour Road Wanchai Hong Kong S A R P R China Tel 852 6621 8337 Fax 852 3020 7554 www dmt asia com sales dmt asia com support dmt asia com DMT Asia China office Rm 28C No 8 Dong Fang Road Lu Jia Zui Financial District Shanghai 200120 P R China TEO 0 215425 1330 Fax 86 21 5877 0063 www dmt asia com sales dmt asia com Support dmt asia com DMT USA Inc 201 East Liberty Street Suite 6 Ann Arbor MI 48104 USA Tel 1 770 612 8014 Fax 1 678 302 7013 www dmt usa com sales dmt usa com supportodmt usa com
Download Pdf Manuals
Related Search