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Operating Manual and User's Guide

1. Specifications Jacket Nylon temperature range 40 to 100 C Sheathing cabling PVC standard Connector termination SMA 905 Fiber core pure fused silica Cladding doped fused silica Fiber profile step index multi mode Numerical aperture 0 22 Recommended minimum bend radius momentary 8 cm long term 16 cm 26 Experiment Tutorial When you are ready to begin your experiment you should have already installed the PC2000 installed OOIChem set up your light source sample holder and connected your fiber from the PC2000 to your light source sample holder Now you are ready to take your measurements Because of the components making up your CHEM2000 and CHEM2000 UV VIS your system is ideal for absorbance and transmission The CHEM2000 can also make relative irradiance measurements If however you wish to utilize your system for other measuring functions additional products might be required Contact an Ocean Optics Applications Scientist for options Absorbance Experiments Absorbance spectra are a measure of how much light is absorbed by a sample The software calculates absorbance Aa using the following equation S D Ax logio e D a Di where Sis the sample intensity at wavelength A Dis the dark intensity at wavelength A R is the reference intensity at wavelength i Common applications include the quantification of chemical concentrations in aqueous or gaseous samples
2. 32 Kinetics Experiments Select Spectrometer Kinetics Configuration from the menu to configure and establish the parameters for a kinetics experiment In the Kinetics Configuration dialog box you can collect spectral data as a function of time from up to 4 single wavelengths and up to two mathematical combinations of these wavelengths A kinetics experiment will not be displayed in the graph unless you choose Spectrometer Spectrometer Configuration from the menu and choose Spectrum amp Kinetics next to Graph and chart display mode This way not only will your kinetics experiment be displayed in the bottom half of the graph area you will also still see a spectrometer channel s real time spectra in the top half of the graph area Kinetics Conty alas HH BESS Greg Daten Lee Te Preset S anpra Inter E dee EN al EU re b EU Ire e 300 Jee d 300 Le bec Si ge er welt Eins el 24 Hi Berean DI To run a kinetics experiment 1 2 3 10 11 In the graph area select acquisition parameters such as integration period averaging and boxcar smoothing values Do not change these parameters for the duration of the kinetics experiment Select Spectrometer Kinetics Configuration from the menu to open the Kinetics Configuration dialog box Enter a Preset Duration value to set the length of time for the entire kinetics process Be sure to select
3. Subtract D ark Selecting this box subtracts the current dark spectrum from the spectra being displayed This command is useful if you are trying to look at a change in an emission spectrum or are trying to eliminate from the spectra fixed pattern noise caused by a very long integration period The subtract dark spectrum function only acts on spectra displayed in Scope Mode Acquire Data M odes Chart Active When Spectrum amp Kinetics is chosen as the Graph and chart display mode in the Spectrometer Configuration dialog box the Chart Active function becomes visible in the display area above the Scan button This function is responsible only for the Kinetics chart By deselecting this function users can use the scan button and collect data in just the spectrum section of the graph If the function is enabled choosing the scan button results in the collection of data in both the spectrum section of the graph and the kinetics section of the graph Scan Stop Button When in Single mode the Scan button acts as a snapshot After selecting the Single mode click on the Scan button to take a scan The button depresses and Stop replaces Scan The button will stay depressed until the scan has been completed the time set in the Integration Period box When in Continuous mode the Scan button continuously takes scans After each integration cycle another scan will immediately begin The button depresses and Stop replaces Scan Click on Stop
4. 4 Now you are ready to calculate the wavelength calibration coefficients In your spreadsheet program find the functions to perform linear regressions if using Quattro Pro look under Tools Advanced Math if using Excel look under Analysis ToolPak 5 Select the true wavelength as the dependent variable Y Select the pixel number pixel number squared and the pixel number cubed as the independent variables X After you execute the regression an output similar to the one shown below is obtained Regression Statistics Multiple R 0 999999831 R Square 0 999999663 Adjusted R Square 0 999999607 Standard Error 0 125540214 Observations 22 intercept Coefficients Standard Error Intercept 190 473993 0 36904 first coefficient X Variable 1 0 36263983 0 001684745 X Variable 2 1 174416E 05 8 35279E 07 X Variable 3 2 523787E 09 e ee second coefficient third coefficient A 6 The numbers of importance are indicated in the above figure You will need to record the Intercept as well as the First Second and Third Coefficients Also look at the value for R squared It should be very close to 1 If it is not you have probably assigned one of your wavelengths incorrectly 7 Select Spectrometer Spectrometer Configuration from the menu Update the wavelength coefficients 38
5. where Sis the sample intensity at wavelength A Dis the dark intensity at wavelength A Ris the reference intensity at wavelength A Common applications include measuring the reflection properties of mirrors anti reflection coatings and measuring the visual properties of the color in paints graphic arts plastics and food products R The components that came with the CHEM2000 and CHEM2000 UV VIS will not allow the user to make reflection measurements A reflection probe is necessary We offer several components for reflection measurements such as a variety of reflection probes a reflection probe holder diffuse reflectance standards and an integrating sphere Contact Ocean Optics for more information To take a reflection measurement 1 Select Scope under Mode of Operation in the software display area Make sure the signal is on scale Adjust acquisition parameters so that the peak intensity of the reference signal is about 3500 counts Take a reference spectrum by first making sure nothing is blocking the light path going to your spectrometer The analyte you want to measure must be absent while taking a reference spectrum Take the reference reading by clicking the Reference button in the software display area This command merely stores a reference spectrum To save a spectrum you must select File Save Spectral Values from the menu Storing a reference spectrum is requisite before the software can calculate reflection spectra 2 Whil
6. To take an absorbance measurement 1 Select Scope under Mode of Operation in the software display area Make sure the signal is on scale Adjust acquisition parameters so that the peak intensity of the reference signal is about 3500 counts Take a reference spectrum by first making sure nothing is blocking the light path going to your spectrometer The analyte you want to measure must be absent while taking a reference spectrum Take the reference reading by clicking the Reference button in the software display area This command merely stores a reference spectrum To save a spectrum you must select File Save Spectral Values from the menu Storing a reference spectrum is requisite before the software can calculate absorbance spectra 2 While still in Scope Mode take a dark spectrum by first completely blocking the light path going to your spectrometer If possible do not turn off the light source If you must turn off your light source to store a dark spectrum make sure to allow enough time for the lamp to warm up before continuing your experiment Take the dark reading by clicking the Dark button in the software display area This command merely stores a dark spectrum To save a spectrum you must select File Save Spectral Values from the menu Storing a dark spectrum is requisite before the software can calculate absorbance spectra 3 Begin an absorbance measurement by first making sure the sample is in place and nothing is blocking the
7. contact Ocean Optics To take a relative irradiance measurement 1 Select Spectrometer Spectrometer Configuration from the menu Next to Color Temp make sure the color temperature in Kelvin of the reference lamp you are going to use is entered here The color temperature of the tungsten halogen light source that came with your CHEM2000 has a color temperature of 3100 Kelvin Click OK Select Scope under Mode of Operation in the software display area Make sure the signal is on scale by adjusting acquisition parameters Take a reference spectrum of your reference lamp Take the reference reading by clicking the Reference button in the software display area This command merely stores a reference spectrum To save a spectrum you must select File Save Spectral Values from the menu Storing a reference spectrum is requisite before the software can calculate relative irradiance spectra While still in Scope Mode take a dark spectrum by first completely blocking light from going to your spectrometer Take the dark reading by clicking the Dark button in the software display area This command merely stores a dark spectrum To save a spectrum you must select File Save Spectral Values from the menu Storing a dark spectrum is requisite before the software can calculate relative irradiance spectra Begin a relative irradiance measurement by first positioning the fiber at the light or emission source you wish to measure Then select Relative
8. filters up to 3 mm thick and accepts color correcting and longpass filters round or square There is no clamp to hold the filter in place To install a filter in the sample holder filter slot loosen the filter clamping screw with an Allen wrench and insert the filter into the filter slot The filter slot can accommodate filters up to 6 mm thick Clamp the filter in place by gently tightening with an Allen wrench Replacing the Bulb on the ISS 2 PEDE A LEND Order a replacement bulb Turn off the lamp and detach the power supply Allow the lamp to cool Remove the fan from the bottom of the lamp by loosening the four screws securing it to the base Two of the four screws also hold the two front legs in place Once the screws are loosened gently pull the fan along with the legs away from the lamp Now that the fan is removed use an Allen wrench to loosen the set screw that is underneath the fan This screw holds the bulb in place You do not need to remove the set screw loosening it is sufficient Locate the two set screws at the back of the lamp one above each back leg These two screws keep the two halves of the lamp together Remove the two screws Gently separate the two halves of the lamp and pull the bulb out of its housing Detach the wire and socket from the lamp leads Remove bulb unit and discard Plug the new bulb into the socket and slide it forward into the front of the lamp as far as it will go Tighten the set sc
9. the following tables supply the necessary information H 1 H eader Pins Slave H eader Pin Function 1 Channel 1 slave 1 2 Channel 2 3 Channel 3 4 Channel 4 5 Channel 5 6 Channel 6 7 Channel 7 8 GND 9 GND 10 SO 11 Reserved 12 Multiple Strobe 13 Single Strobe 14 Reserved 15 F read ROG 16 F clock J2 D SUB 15 Connectors Pin Function 1 Strobe Single Fire 2 Strobe Multiple Fire 3 5 VDC 4 External Hardware Trigger 5 External Synchronization 6 Analog Channel 7 Input 7 Analog Channel 6 Input 8 External Software Trigger 9 Analog Channel 1 10 Ground 11 Analog Channel 4 12 Analog Channel 5 13 Digital Out 0 Strobe Enable or Lamp On Off 14 Analog Channel 3 15 Analog Channel 2 36 Appendix C Calibrating the Wavelength of Your Spectrometer The following describes how to calibrate the wavelength of your spectrometer Though each spectrometer is calibrated before it leaves Ocean Optics the wavelength for all spectrometers will drift slightly as a function of time and environmental conditions You are going to be solving the following equation which shows that the relationship between pixel number and wavelength is a third order polynomial A I C p Op Op where A is the wavelength of pixel p J is the wavelength of pixel 0 C is the first coefficient nm pixel C is the second coeffi
10. Irradiance under Mode of Operation in the software display area Click on the Scan button in the display area to take a scan If Single is selected only one scan will be taken If Continuous is selected the spectrometer will continuously take scans until you click on the Stop button To save the spectrum select File Save Spectral Values from the menu Pb If at any time any sampling variable changes including integration period averaging boxcar smoothing distance from light source to sample etc you must store a new reference and dark spectrum 30 Concentration Experiments The absorbance of a solution is related to the concentration of the species within it The relationship known as Beer s Law is A amp cl where A is the absorbance at wavelength A is the extinction coefficient of the absorbing species at wavelength A c is the concentration and is the optical pathlength Concentration is the amount of a specified substance in a solution Graphs of absorbance vs concentration are known as Beer s Law plots These are prepared by measuring the light absorbed by a series of solutions with different known concentrations The length of the sample such as the path length of our cuvette holder and the wavelength chosen for monitoring the amount of light absorbed are constants A linear plot from taking scans of these standard solutions with known concentrations is then obtained The plot is then used to determine
11. above the 12V label For users of European version wall transformers plug the transformer into a standard 220 V outlet At this time the green LED indicator light on the front of the lamp will be lit This indicator light only means that the lamp is receiving power not that the deuterium and tungsten bulbs are on Operating the ISS UV VIS Manually Find the switch on the back of the ISS UV VIS There are three positions On Off and Remote For Manual operation move the switch to the On position There can be up to a 1 5 second delay between switching the lamp to on and the bulbs igniting If the lamp has not been used recently the deuterium bulb may take up to 60 seconds to ignite 3 For 0 3 peak to peak stability allow 30 minutes warm up time before taking your measurements Operating the ISS UV VIS through Software Take the 15 pin accessory cable and plug one end into the PC2000 installed into your computer Connect the other end of the accessory cable into the back of the ISS UV VIS 2 Find the switch on the back of the ISS UV VIS There are three positions On Off and Remote For Software operation move the switch to the Remote position Moving the switch to the Remote position enables you to control the lamp through the software whether you are using OOIChem or OOIBase32 3 When using OOIChem select Spectrometer Enable Strobe from the menu to turn the light source in the ISS UV VIS on and off 4 When you want to control
12. choose a cursor style and a point style You can also choose a color for the cursor and whether or not to display the name of the channel the cursor is currently reporting Finally you can bring the cursor to the center of the spectrum or center the spectrum around the cursor s current position Text Box This box allows you to enter an operator name and any other text to identify your experiment This text appears in your data files You can edit this text at any time Acquisition Parameters Integration Period Enter a value to set the integration period in milliseconds for an active spectrometer channel The integration period of the spectrometer is analogous to the shutter speed of a camera The higher the value specified for the integration period the longer the detector looks at the incoming photons If your scope mode intensity is too low increase this value If the intensity is too high decrease the value While watching the graph trace in Scope Mode adjust the integration period and other acquisition parameters until the signal intensity level is approximately 3500 counts Average Enter a value to implement a sample averaging function that averages the specified number of spectra The higher the value entered the better the signal to noise ratio The S N improves by the square root of the number of scans averaged Boxcar Smooth Enter a value to implement a boxcar smoothing technique that averages across spectral
13. concentration experiment See pages 31 32 for step by step instructions on calculating concentrations Calculate Calibration Curve Concentration is the amount of a specified substance in a solution In order to calculate concentration you must take absorbance measurements of a series of solutions with different known concentrations The length of the sample and the wavelength chosen for monitoring the amount of light absorbed are constants Then a linear plot from taking these scans is obtained This Calibration Curve is used to determine the unknown concentrations See pages 31 32 for step by step instructions on calculating concentrations and on using this dialog box Enable Strobe This function allows you to enable or disable the triggering of external strobes through the spectrometer You would only select Spectrometer Strobe Enable from the menu if you were operating an external strobe source The CHEM2000 and CHEM2000 UV VIS systems do not include a strobe light source However the ISS UV VIS that comes with the CHEM2000 UV VIS can be turned off and on through the software and this function Spee hie ee oral ud abe Spectrometer C onfiguration Intercept From the Wavelength Calibration Report that came with your system enter the Intercept under the Master column Though the CHEM2000 and CHEM2000 UV VIS come with only a Master spectrometer channel additional spectrometer channels can be added at any time Contact an Application
14. converter For the PC2000 there is only one bank of switches on the A D converter the Base Address may be changed via the first 6 switches and the IRQ may be changed via the last 3 switches See Appendix A on page 34 for switch positions 5 Insert the PC2000 into an ISA bus slot Make sure the connections are snug and restart your computer Step 2 Install OOIChem Software Before installing OOIChem make sure that no other applications are running 1 Insert Disk 1 into your floppy drive When prompted insert Disks 2 and 3 Execute Setup exe 2 At the Welcome dialog box click Next gt 3 At the Destination Location dialog box you can choose Browse to pick a destination directory Click Next gt 4 At the Backup Replaced Files dialog box select either Yes or No We recommend selecting Yes If you select Yes you can choose Browse to pick a destination directory Click Next gt Select a Program Manager Group Click Next gt At the Start Installation dialog box click Next gt At the Installation Complete dialog box choose Finish gt When prompted to do so restart your computer when the installation is complete IR Step 3 Configure OOIChem Software After you restart your computer navigate to the OOIChem icon and select it Now that the PC2000 and software have been installed you need to configure your software The first time you run OOIChem after installation you must follow sever
15. data This method averages a group of adjacent detector elements A value of 5 for example averages each data point with 5 points or bins to its left and 5 points to its right The greater this value the smoother the data and the higher the signal to noise ratio However if the value entered is too high a loss in spectral resolution results The S N improves by the square root of the number of pixels averaged Reference Scan Selecting the Reference button activates a prompt to make sure your light is on You then must choose to either Store or Cancel your reference scan A reference spectrum is taken with the light source on and a blank in the sampling region Storing a reference spectrum is requisite before the software can calculate absorbance transmission and relative irradiance spectra This command merely stores a reference spectrum To permanently save the reference spectrum to disk select File Save Spectral Values from the menu Dark Scan Selecting the Dark button activates a prompt to make sure the light path is blocked You then must choose to either Store or Cancel your dark scan A dark spectrum is taken with the light path to the spectrometer blocked Storing a dark spectrum is requisite before the software can calculate absorbance transmission and relative irradiance spectra This command merely stores a dark spectrum To permanently save the reference spectrum to disk select File Save Spectral Values from the menu
16. light going to your sample Then select Absorbance under Mode of Operation in the software display area Click on the Scan button in the display area to take a scan If Single is selected only one scan will be taken If Continuous is selected the spectrometer will continuously take scans until you click on the Stop button To save the spectrum select File Save Spectral Values from the menu Pb If at any time any sampling variable changes including integration period averaging boxcar smoothing distance from light source to sample etc you must store a new reference and dark spectrum 27 Transmission Experiments Transmission is the percentage of energy passing through a system relative to the amount that passes through the reference Transmission Mode is also used to show the portion of light reflected from a sample Transmission and reflection measurements require the same mathematical calculation The transmission is expressed as a percentage TA relative to a standard substance such as air The software calculates Ta or Ra by the following equation S D eut x100 R D where Sis the sample intensity at wavelength A D is the dark intensity at wavelength A R is the reference intensity at wavelength A Common applications include measurement of transmission of light through solutions optical filters optical coatings and other optical elements such as lenses and fibers Pb For transmission of light through s
17. the menu to print a spectrum or select File Print Kinetics from the menu to print kinetics data Exit Select File Exit from the menu to quit OOIChem A message box appears asking you if you are sure you want to exit the software Edit Menu Functions Clear Spectrum Overlays Select Edit Clear Spectrum Overlays from the menu to remove static spectra from the graph Clear Kinetics Values Select Edit Clear Kinetics Values from the menu to clear both the kinetics values from the chart and to clear the kinetics traces A message box then appears asking if you are sure you want to clear the kinetics chart Autoscale X The Autoscale X function automatically adjusts the horizontal scale of a current graph so the entire horizontal spectrum fills the display area Autoscale Y The Autoscale Y function automatically adjusts the vertical scale of a current graph so the entire vertical spectrum fills the display area Show Kinetics V alues When setting up your kinetics experiment you 600 o 800 o x 1 0 must first select Spectrometer Spectrometer Configuration from the menu and make sure that Spectrum amp Kinetics is selected next to Graph and chart display mode Then configure your experiment by selecting Spectrometer Kinetics Configuration from the menu When you select your wavelengths the values from these wavelengths will be displayed above the kinetics chart if this function is enabled Show
18. the unknown concentrations of substances in solutions In order to discover the unknown concentration of a substance in a solution you must first take spectral scans of a series of solutions with different known concentrations of the same substance You begin this process by taking an absorbance spectrum of the solution with the highest known concentration 1 Select Scope under Mode of Operation in the software display area Make sure the signal is on scale Adjust acquisition parameters so that the peak intensity of the reference signal is about 3500 counts Take a reference spectrum by first making sure nothing is blocking the light path going to your spectrometer The solution with the highest known concentration you want to measure must be absent while taking a reference spectrum Take the reference reading by clicking the Reference button in the software display area To save the spectrum select File Save Spectral Values from the menu 2 While still in Scope Mode take a dark spectrum by first completely blocking the light path going to your spectrometer If possible do not turn off the light source If you must turn off your light source to store a dark spectrum make sure to allow enough time for the lamp to warm up before continuing your experiment Take the dark reading by clicking the Dark button in the software display area To save the spectrum select File Save Spectral Values from the menu 3 Take the solution with the highest kn
19. thousand times smaller and ten times less expensive than previous systems Due to this dramatic reduction in size and cost of optical sensing systems applications once deemed too costly or impractical using conventional spectrometers were now feasible CHEM 2000 and CHEM 2000 UV VIS Spectrophotometer Systems The CHEM2000 and CHEM2000 UV VIS Spectrophotometer Systems utilize the optical bench of our second generation miniature fiber optic spectrometer the S2000 by mounting it onto an A D converter and turning the system into a PC plug in spectrometer The CHEM2000 consists of four basic elements a CHEM2000 BW PC2000 PC Plug in Fiber Optic Spectrometer 350 i 900 nm a tungsten halogen light source with integrated cuvette holder a 400 um optical fiber and OOIChem operating software CHEM2000 UV VIS The CHEM2000UV VIS also consists of four basic elements the PC2000 UV VIS PC Plug in Fiber Optic Spectrometer 200 850 nm a miniature deuterium tungsten light source with integrated cuvette holder a 300 um solarization resistant optical fiber and OOIChem operating software The light source supplies light to the sample The light transmitted through the sample is collected and sent to the spectrometer via the fiber The spectrometer measures the amount of light at each wavelength in the sampled spectrum The A D converter on which the spectrometer is mounted transforms the analog data from the spectrometer into digital infor
20. time has passed Select File Save Kinetics Values from the menu to save a tab delimited ASCII file with the spectrometer s serial number active channel and acquisition parameters in a header This file can be opened with any text or spreadsheet editor 33 Appendix A Changing the Settings on the PC2000 Base Address Settings To change the Base Address settings on the PC2000 find the bank of switches on the A D converter The Base Address may be changed via the first 6 switches the IRQ may be changed via the last 3 switches Switches in the OFF position have the decimal values shown Switches in the ON position have a value of zero The Base Address is the sum of the values of the switches In the default setting switches 5 and 6 are added to give a value of 768 A few of the many combinations for Base Address settings are below After you have changed the switches reinstall the card and change the software settings to match the hardware settings See pages 6 8 for instructions The gray block indicates the position of the switch Switch is in the on upward position Example 768 decimal Hex300 0x300 Default Setting Switch 1 2 3 4 5 6 ON OFF Decimal equivalent 16 32 64 128 256 512 Value as shown 256 512 Example 784 decimal Hex310 0x310 Switch 1 2 3 4 5 6 ON OFF Value as shown 16 256 512 Example 800 decima
21. to halt the scanning process and discontinue acquiring data Scaling the Graph You can change the vertical and or horizontal scales of the graph by simply clicking on an X and Y endpoint and manually typing in a value The graph will then resize itself File Menu Functions Save Spectral Values Select File Save Spectral Values from the menu to save the current spectrum Text box entries and acquisition parameters are included in the headers of these files You can then use these files as overlays or import them into other software programs such as Microsoft Excel Save Kinetics Values Select File Save Kinetics Values from the menu to save kinetics data Text box entries and acquisition parameters are included in the headers of these files You can then import them into other software programs such as Microsoft Excel Open Spectrum Overlay Select File Open Spectrum Overlay from the menu to open a dialog box that allows you to open a previously saved spectrum and to open it as an overlay a static spectrum while still acquiring live data You can open up to 8 overlays in the graph Open Kinetics Values Select File Open Kinetics Values from the menu to open a dialog box that allows you to open a previously saved kinetics chart Printer Setup Select File Printer Setup from the menu to select and configure a printer for printing graphical spectra or kinetics data Print Spectra and Kinetics Select File Print Spectra from
22. I O Port button Find an available I O Range Base Address a number or range of numbers unassigned to a device The number is in hexadecimal Note these available settings When you first run OOIChem you must enter these values in the Configure Hardware dialog box E Wein NI Dissen LER Lei Ia Ki weet RI ipia VLEET With Windows NT devices cannot share IRQs each device must be assigned a unique IRQ Install the PC 2000 Turn off the computer and remove the computer cover Ground yourself to the computer chassis or power supply Remove the PC2000 spectrometer A D card combination from its static shielded bag If necessary change the position of the switches on the A D converter Position the switches to match the available settings you found in the previous section numbers not being used by other hardware devices See Appendix A on page 34 for switch setting positions Find an open ISA bus slot and remove the slot protector Insert the PC2000 into an available expansion slot on the motherboard connector by gently rocking the card into the slot Make sure the card is fully seated in the motherboard before screwing the tab on the PC2000 to the computer Do not bend the card or move it from side to side once it is seated in the slot If you have a CHEM2000 take your 400 um fiber that came with your system and screw one end into the SMA connector on your PC2000 If you have a CHEM2000 UV VIS take your 300 um solarization r
23. IChem is that users can control the parameters for all system functions such as acquiring data designing the graph display and configuring the cursor Additional features of OOIChem include the ability to save data as ASCII files and to store and retrieve sample spectra OOIChem software will be updated and improved continuously To obtain free upgrades visit out web site at www oceanoptics com Software_Updates asp In order to download free upgrades you will need the password found on the back of your Software and Resources Library CD If you find that you need more advanced spectrometer operating software we have included free of charge OOIBase32 a sophisticated 32 bit user customizable advanced acquisition program See the included OOIBase32 manual for a list of functions and features Display Functions Several functions are accessed not through the menu but through buttons and task bars directly on the display screen on the top and to the right of the graph area The resolution of your computer s monitor must be 800 x 600 or better to view OOIChem software From the display screen you can choose a mode of operation configure the cursor configure the graph enter acquisition parameters choose a mode to acquire data take reference dark and sample scans of your sample and scale the graph Spectrometer Channel Selection The Spectrometer Channel area allows you to animate the window for a specific spectrometer channe
24. IS Caution KA The beam emerging from the ISS UV VIS produces low levels of ultraviolet radiation Direct eye contact could cause eye injury Safety eyewear is recommended S Never look directly into the light source sight down the beam into the source or stare at the diffuse reflected beam kA Dangerous voltages present NO serviceable parts inside unit To replace bulbs contact Ocean Optics R This instrument should not be used for any clinical or diagnostic purposes fs Handle with care Dropping the instrument may cause permanent damage Operation Adjusting the Fit of the Cuvette for the ISS UV VIS The ISS UV VIS is designed to hold 1 cm square cuvettes When properly adjusted the cuvette should fit snugly into the holder 1 Locate the two ball plunger screws 2 Use the screwdriver to loosen the two ball plunger screws until the ball end of each screw is just visible in the holder 3 Insert your cuvette into the holder 4 Gently tighten the screws until the ball contacts the cuvette and starts to compress Do not over tighten Setting Up the ISS UV VIS 1 Attach the 300 um solarization resistant optical fiber that came with you CHEM2000 UV VIS to the SMA connector on the front of the sample holder and attach the other end of this fiber to the SMA connector on the PC2000 installed in your computer 23 2 Plug the wall transformer into a standard 110 V outlet Plug the 12 V output into the back of your ISS UV VIS
25. Legends Select Edit Show Legends to enable or disable the legends for the spectral trace overlays and kinetics traces When the legends are displayed you can opt to configure the traces by simply clicking on the legend trace you want to configure You have the opportunity to choose from several aesthetic functions such as the plot design of the spectrum the point style used in the spectrum the line style and width desired color of the plot and a bar plot design You can also choose to fill the baseline in the spectrum Utilize this function to differentiate one spectral trace from another Active File 1 File 2 File 3 File 4 File 5 File 6 File 7 File 8 WEE Spectrometer Menu Functions Scan When the Single mode is selected in the display screen the Scan menu function acts as a snapshot After selecting the Single mode select Spectrometer Scan from the menu to take one scan of the sample When the Continuous mode is selected in the display screen select Spectrometer Scan from the menu to continuously take scans Select Concentration Wavelength This function is used when calculating the unknown concentration of a substance in a solution You select this function after you take an absorbance measurement of a standard solution with a known concentration Choose the wavelength of the highest peak in your absorbance spectrum Then select Spectrometer Calculate Calibration Curve from the menu and complete the rest of your
26. Operating Manual and User s Guide CHEM2000 and CHEM2000 UV VIS Miniature Fiber Optic Spectrophotometers Ocean Optics Inc 380 Main Street Dunedin FL 34698 727 733 2447 727 733 3962 fax For the latest information consult our web site www OceanOptics com Or e mail our Technical Service Department TechSupport OceanOptics com A MN cean K ptics Inc 021400 Copyright 2000 Ocean Optics Inc All rights reserved No part of this publication may be reproduced stored in a retrieval system or transmitted by any means electronic mechanical photocopying recording or otherwise without written permission from Ocean Optics Inc This manual is sold as part of an order and subject to the condition that it shall not by way of trade or otherwise be lent re sold hired out or otherwise circulated without the prior consent of Ocean Optics Inc in any form of binding or cover other than that in which it is published Trademarks Microsoft Windows Windows 95 Windows 98 and Windows NT are either registered trademarks or trademarks of Microsoft Corporation Limit of Liability Every effort has been made to make this manual as complete and as accurate as possible but no warranty or fitness is implied The information provided is on an as is basis Ocean Optics Inc shall have neither liability nor responsibility to any person or entity with respect to any loss or damages arising from the information
27. Q and the Input output Base Address When you first run OOIChem you must enter these values in the Configure Hardware dialog box Remember that Input output settings are expressed in hexadecimal EST Emer mE View Reitti Fiese Rasaan View Fetoa Aeren Bearne fr O Bech merap cute ld D nian pease fa d Drei green Ster oh C Lee gies j C Heen i inpia ta T Baroy Gear Hacker ud Te per IS IG ie ee Eiaeia DEZ SCH Roden Cordi OR It Pirie Peet LECH Deia VED of er Plaid Red US Pregreseresble inden Garn aaa Prot TAT DS BE AT DPE ii Direct Biraja Fre D bk C rett Biusn art AT SO RABE ih Dore D Pepe Poet LPT DD IER Hadad Free Dr Coia Feta CMS Mead ez ek a CGO edd Daa PO IDEE Code D tz op a ne a a ee su For most computers the default settings on our A D converters work well In the picture at left on page 6 it appears that the Printer occupies IRQ Setting 07 but for most computers our A D converters can share the IRQ 07 setting with a printer and conflicts will not arise All computers have multiple Base Address Input output settings from which to choose Windows NT Users Find Available Base Address and IRQ Settings Go to Start Programs Administrative Tools Common Windows NT Diagnostics In the Windows NT Diagnostics dialog box click on the Resources tab Select the IRQ button Find an available IRQ anumber unassigned to a device Select the
28. adiance Experiments Irradiance is the amount of energy at each wavelength from a radiant sample In relative terms it is the fraction of energy from the sample compared to the energy collected from a lamp with a blackbody energy distribution normalized to 1 at the energy maximum Relative irradiance is calculated by the following equation S Dy al A HA where Bh is relative energy of the reference calculated from the color temperature in Kelvin Sis the sample intensity at wavelength A Dis the dark intensity at wavelength A Ris the reference intensity at wavelength A Common applications include characterizing the light output of LEDs incandescent lamps and other radiant energy sources such as sunlight Also included in irradiance measurements is fluorescence in which case the spectrometer measures the energy given off by materials that have been excited by light at a shorter wavelength Li The components that came with the CHEM2000 UV VIS will not allow the user to make relative irradiance measurements Only CHEM2000 users can perform relative irradiance measurements In order to make relative irradiance measurement the reference spectrum must be made in Scope Mode with a blackbody light source of known color temperature This color temperature is needed in order to calculate relative irradiance The light source that comes with the CHEM2000 UV VIS is not a blackbody light source with a known color To purchase a blackbody light source
29. al prompts to configure your system before taking measurements Hardware Configuration The Configure Hardware dialog box opens when you first run OOIChem The parameters in this dialog box are usually set only once when OOIChem is first installed and the software first opens 1 Under Spectrometer Type choose S2000 PC2000 2 Under A D Converter Type choose ADC1000 PC2000 3 Under Base Address choose the same setting as the dip switches on the A D converter and the same available setting you found in your computer Remember that the Input output Range Base Address you selected was expressed in hexadecimal In this dialog box the base address is given in decimal followed by the hexadecimal equivalent in parenthesis For example 768 0x0300 gives the base address as 768 decimal and 300 hexadecimal 4 Under IRQ choose the same setting as the dip switches on the A D converter and the same available setting you found in your computer 5 For your setup only these parameters apply to your system Click OK You can always change these settings once OOIChem is fully operational by selecting Spectrometer Hardware Configuration Spectrometer Configuration At this point OOIChem should be acquiring data from your spectrometer There should be a dynamic trace responding to light near the bottom of the displayed graph Now that OOIChem is running you need to configure your system Select Spectrometer Spectrometer Configurat
30. cient nm pixel and C is the third coefficient nm pixel You will be calculating the values for J and the three Cs Setting Up To re calibrate the wavelength of your spectrometer you will need the following e A light source that produces spectral lines Ocean Optics HG 1 Mercury Argon lamp is ideal for this purpose If you do not have an HG 1 you will need a spectral line source that produces several at least 4 6 spectral lines in the wavelength region of your spectrometer e Your spectrometer e An optical fiber the fibers that came with your CHEM2000 and CHEM2000 UV VIS will work fine e Either a spreadsheet program Excel or Quattro Pro for example or a calculator that performs third order linear regressions If you are using Microsoft Excel choose Tools Add Ins from the menu and check AnalysisToolPak and AnalysisTookPak VBA Calibrating the Wavelength of Your Spectrometer 1 After placing OOIChem into Scope Mode take a spectrum of your light source that produces spectral lines Adjust the integration period until there are several peaks on the screen that are not off scale 2 Move the cursor to one of the peaks and carefully position it so that it is at the point of maximum intensity Record the pixel number that is displayed in the cursor function bar located above right of the graph Repeat this step for all of the peaks in your spectrum 3 Using your spreadsheet create a table like the one shown on the nex
31. contained in this manual Table of Contents lee IO 1 QUICK SIE EE A Installing the PC2000 and OOIChem Gottware cece eee 6 Using OO Chem Software eeennenenennennennenennennenn 9 Display TT EE 10 Spectrometer Channel Gelectlon nnn nnnnen nenn 10 Mode of Operation sde eased ash dee Tasca ev reales 10 GUrSOF lie tel NEE 11 RE 12 ACQUISITION Parameters osaan ai a a a a ea E N 12 Reference Scan TEE 12 DANO SCAN EE E 12 Subtract RE 13 ee UN EE 13 Scaling the Graph iri aussie anal heen Agia Ae eee 13 File Menu Functions tee guerre 14 Save Spectral Vleit ett una Sege gidd Dee aAa dE 14 SAVE le 14 Open Spectrum Overlay ssssssnnsennnnnnnnannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnannnnnnnnnnnnann 14 Open Kinetics Values 0 2 ce cccecceeeecceceeeeeeeaeeeeeeeeeeeeeceaeeeeaaeseneecaeeecaaeeeeaaesdeneeseeessaaeseeaeeeseneesaees 14 Selen EE EE 14 Print Spectra and Kinetics ne a le Gn ea 14 Ee enge aa cena th ebe ET N EE OEN EE ege eg E E ies 14 Edit Menu UE CEN ENS a2 a a a EN 15 Clear Spectrum Overlays cccccecceesseceeeeeceeeeeeaeeeeaeeceeeecaaeeseaaesaeeeseaeeesaaeseeaeeseeaeessaeseeneeesaes 15 Clear Kinetics V ltee entsteet seiten een 15 aleet EEN 15 AutoscaleY E 15 Show Kinetics Values 22 ue Es eae ieee 15 lee Ee EE 15 Spectrometer Menu Functions 4snnnennenennenennenennenennnnnnennnennn 16 e ET 16 Select Concentration Wavelengih nenn 16 Calc
32. ctor female Graph and chart display mode If you choose Spectrum Only spectra from one real time spectrometer channel and up to 8 overlays can be displayed in the graph area If you choose Spectrum amp Kinetics spectra from one real time spectrometer channel and up to 8 overlays can be displayed in the in the top half of the graph area A kinetics chart monitoring values from up to 4 wavelengths and 2 of their arithmetic calculations can be displayed in the in the bottom half of the graph area if the Chart Active function is enabled Flash Delay The value entered here sets the delay in milliseconds between strobe signals sent out of the spectrometer This function allows you to enable or disable the triggering of external strobes through the spectrometer You would only enter a value in this box if you were operating an external strobe source The CHEM2000 and CHEM2000 UV VIS systems do not include a strobe light source Color Temperature This box allows you to enter the color temperature in Kelvin of your light source In order to calculate relative irradiance the reference spectrum must be made in Scope Mode with a blackbody light source of known color temperature This data is necessary for the software to complete calculations for relative irradiance measurements For CHEM2000 users your lamp has a color temperature of 3100 Kelvin CHEM2000 UV VIS users cannot make relative irradiance measurements because the light source
33. e integration period are still set in the software You should use this mode if you are using a continuous light source and its intensity is constant before during and after the trigger Ip In order for you to use the External Software Triggering option it is imperative that you know the specifications and limitations of your triggering device The design of your triggering device may prevent you from using the external software triggering mode as it is described here To use the External Software Trigger Mode 1 Supply a line from your triggering device to Pin 3 of the J2 Accessory Connector on the PC20000 to provide the positive voltage S VDC to the spectrometer See figure for pin location We do not advise using an outside source to supply the voltage as it is based on a referenced ground and your reference may be different from ours Using Pin 3 to supply voltage ensures that the spectrometer will receive the appropriate voltage for the trigger event 2 Supply a line from Pin 8 of the J2 Accessory Connector to your triggering device See figure for pin location 3 Set your acquisition parameters in the software 4 Select Spectrometer Spectrometer Configuration from the menu and choose External Software Trigger 5 Once you select External Software Trigger it will appear on your computer that your spectrometer is unresponsive Instead it is waiting for the trigger Activate your triggering device J2 D SUB 15 Accessory Conne
34. e spectra are a measure of the intensity of a light source relative to a reference emission source Relative irradiance is calculated by the following equation S D SCH ao AT Di where Bis relative energy of the reference calculated from the color temperature in Kelvin Sis the sample intensity at wavelength A D is the dark intensity at wavelength A R is the reference intensity at wavelength A Concentration Concentration is the amount of a specified substance in a solution Graphs of absorbance vs concentration are known as Beer s Law plots These are calculated by first measuring the light that is absorbed from a series of solutions with different known concentrations The length of the sample such as the path length of our cuvette holder and the wavelength chosen for monitoring the amount of light absorbed are constants Then a linear plot derived from the scans of these standard solutions with known concentrations is obtained The plot is then used to determine the unknown concentrations of solutions See the Experiment Tutorial beginning on page 27 The absorbance of a solution is related to the concentration of the species within it The relationship known as Beer s Law is Au EA where A is the absorbance at wavelength A is the extinction coefficient of the absorbing species at wavelength A c is the concentration and is the optical pathlength Cursor Function Bar Sign When the is selected t
35. e still in Scope Mode take a dark spectrum by first completely blocking the light path going to your spectrometer If possible do not turn off the light source If you must turn off your light source to store a dark spectrum make sure to allow enough time for the lamp to warm up before continuing your experiment Take the dark reading by clicking the Dark button in the software display area This command merely stores a dark spectrum To save a spectrum you must select File Save Spectral Values from the menu Storing a dark spectrum is requisite before the software can calculate reflection spectra 3 Begin a reflection measurement by first making sure the sample is in place and nothing is blocking the light going to your sample Then select Transmission under Mode of Operation in the software display area The mathematics required to calculate reflection measurements are identical to those necessary to compute a transmission spectrum Click on the Scan button in the display area to take a scan If Single is selected only one scan will be taken If Continuous is selected the spectrometer will continuously take scans until you click on the Stop button To save the spectrum select File Save Spectral Values from the menu Pb If at any time any sampling variable changes including integration period averaging boxcar smoothing distance from light source to sample etc you must store a new reference and dark spectrum 29 Relative Irr
36. ent at a sharp angle A bending radius of less than 1 is dangerous Keep connectors and probe tips covered when the fibers are not being used Clean the ends of the fibers with lens paper and distilled water alcohol or acetone Avoid scratching the surface Do not immerse fiber ends in caustic materials or other solutions that can damage glass Specifications Jacket Nylon temperature range 40 to 100 C Sheathing cabling PVC standard Connector termination SMA 905 Fiber core pure fused silica Cladding doped fused silica Fiber profile step index multi mode Numerical aperture 0 22 Recommended minimum bend radius momentary 10 cm long term 16 cm 22 CHEM2000 UV VIS Sampling Components ISS UV VIS Integrated Sampling System The ISS UV VIS Integrated Sampling System that comes with the CHEM2000 UV VIS is a combination of a RF deuterium source with a tungsten halogen bulb connected to a cuvette holder for 1 cm cuvettes The cuvette holder attaches directly to the light source and has a 5 mm diameter f 2 collimating lens to collect the light and funnel it to the solarization resistant optical fiber that also comes with the CHEM2000 UV VIS The ISS UV VIS can be operated manually or through the software Parts Included e ISS UV VIS Deuterium Tungsten Light Source Cuvette Holder e Power cord for connecting the ISS UV VIS to outlet e 15 pin accessory cable for software control of the ISS UV V
37. es 6 8 for details 1 about changing the IRQ and Base Address values and about this dialog box EA CHEM2000 Sampling Components ISS 2 Integrated Sampling System The CHEM2000 comes with a light source sample holder combination called the ISS 2 Integrated Sampling System It is a fully integrated tungsten halogen light source and 1 cm square cuvette holder that couples to the PC2000 with optical fiber to create a small footprint system from 350 to 900 nm The ISS 2 has a 900 hour bulb with a color temperature of 3100 Kelvin and has a 5 mm diameter f 2 collimating lens In the ISS 2 the fan is not enclosed in the base it is exposed and should be handled with care Parts Included ISS 2 light source and cuvette holder assembly 12VDC wall transformer for providing power 1 cm square plastic cuvette for holding samples Screwdriver for adjusting the fit of the cuvette Allen wrench for adjusting the collimating lens Caution The light source becomes HOT during operation Handle with care DO NOT insert plastic or flammable materials in the filter slot The materials could melt or ignite The unit could ignite flammable materials that come in contact with the metal housing Ze X Ze Xa There is an exposed fan on the bottom of the light source sample holder Handle with care Operation Adjusting the Fit of the Cuvette for the ISS 2 The ISS 2 is designed to hold 1 cm square cuvettes When properly adjusted the cuvette should f
38. esistant fiber that came with your system and screw one end into the SMA connector on your PC2000 Reinstall the cover Install OOIChem The resolution of your computer s monitor must be 800 x 600 or better to use OOIChem software Before installing OOIChem make sure that no other applications are running 1 Insert Disk 1 into your floppy drive When prompted install Disks 2 and 3 Execute Setup exe 2 At the Welcome dialog box click Next gt 3 At the Destination Location dialog box you can choose Browse to pick a destination directory Click Next gt 4 At the Backup Replaced Files dialog box select either Yes or No We recommend selecting Yes If you select Yes you can choose Browse to pick a destination directory Click Next gt At the Installation Complete dialog box choose Finish gt When the installation is complete you must restart your computer au Run OOIChem After you restart your computer navigate to the OOIChem icon and select it The first time you run OOIChem after the installation you must enter data into the Configure Hardware dialog box Configure Hardw are The Configure Hardware dialog box opens automatically the first time you run the software The parameters in this dialog box are usually set only once when OOIChem is first installed and the software first opens 1 Under Spectrometer Type choose 2000 PC2000 2 Under A D Converter Type choose Ocean Opt
39. exit Notepad Restart OOIChem You should now see the Configure Hardware dialog box Using OOIChem Software OOICHEM OPERATING SOFTWARE provides users with a real time interface to a variety of acquisition signal processing display and measurement functions The 32 bit software program operates with Windows 95 98 and Windows NT and performs basic absorbance emission and kinetics functions which are presented in an easy to follow format that is ideal for undergraduate level students and beginning spectroscopists OOIChem software provides users with five different modes of operation Scope Absorbance Transmission Relative Irradiance and Concentration In addition the software allows users to control data acquisition features such as integration period averaging and boxcar smoothing directly from the spectral graph display Users can acquire data by taking manual single scans or by running continuous scans and add into the spectral window as many as 8 previously saved overlay spectra Users can also perform kinetics experiments which allows users to monitor and report up to 4 single wavelengths or up to 2 calculated values from a combination of wavelengths for example an absorbance value of 400 minus an absorbance value of 700 A kinetics chart displays the time series When the experiment is complete the data can be exported to an ASCII file for additional processing Another exciting feature of OO
40. fore the software can calculate transmission spectra Begin a transmission measurement by first making sure the sample is in place and nothing is blocking the light going to your sample Then select Transmission under Mode of Operation in the software display area Click on the Scan button in the display area to take a scan If Single is selected only one scan will be taken If Continuous is selected the spectrometer will continuously take scans until you click on the Stop button To save the spectrum select File Save Spectral Values from the menu Pb If at any time any sampling variable changes including integration period averaging boxcar smoothing distance from light source to sample etc you must store a new reference and dark spectrum 28 Reflection Experiments Reflection is the return of radiation by a surface without a change in wavelength The reflection may be e Specular in which the angle of incidence is equal to the angle of reflection e Diffuse in which the angle of incidence is not equal to the angle of reflection Every surface returns both specular and diffuse reflections Some surfaces may return mostly specular reflection others more diffuse reflection The glossier the surface the more specular the reflection Reflection is expressed as a percentage Ra relative to the reflection from a standard substance such as our WS 1 white reference for a diffuse reflection measurement S D ap LN 100 R D
41. h it is coupled i e though the spectral range of the ISS 2 goes to 2 um it can only see to 900 nm when used with the PC2000 that came with your CHEM2000 The amount of time it takes for the lamp to achieve a stabilized output will vary A fan cools the lamp therefore the lamp may take much less than 30 minutes to stabilize 21 400 um Diameter Optical Fiber The 400 um diameter optical fiber that comes with your CHEM2000 is a single strand optical fiber The active part of the fiber consists of a silica core surrounded by a silica cladding material The fiber is very fragile and if not protected by a suitable buffer material would be nearly useless for most applications The buffer materials are polymer coatings that provide mechanical strength Cabling further protects the buffer coated fiber Our standard laboratory cabling is blue PVC The ends of the fibers are cleaved epoxied into the SMA connectors and polished Follow the guidelines below when handling your fiber mo 7797 P P P Care and Use G uidelines When fibers break they stop transmitting light Inspect fibers by eye to determine if light is being transmitted Bending the fiber will cause attenuation To minimize this effect add extra strain relief to both ends of the fiber Do not exceed the temperature specifications for the materials involved 200 C for the fiber 100 C for PVC cabling 100 C for standard epoxy Do not allow the fiber to be b
42. h of Your Spectrometer 37 Introduction Ocean Optics miniature fiber optic spectrometers and accessories have revolutionized the analytical instrumentation market by dramatically reducing the size and cost of optical sensing systems More than 10 000 Ocean Optics spectrometers have been sold worldwide striking evidence of the far reaching impact of low cost miniature components for fiber optic spectroscopy Diverse fields such as research and development industrial process control medical diagnostics environmental monitoring and of course education have benefited from access to Ocean Optics technology In fact Ocean Optics has its roots in education It formed in 1989 when Florida university researchers developed a fiber optic pH sensor as part of an instrument designed to study the role of the oceans in global warming The researchers soon formed Ocean Optics Inc and their ingenious work earned a Small Business Innovation Research grant from the U S Department of Energy While designing the pH monitoring instrument the researchers wanted to incorporate with their sensor a spectrometer small enough to fit onto a buoy and were surprised to discover none existed So they built their own In 1992 the founders of Ocean Optics filled a substantial need in the research community and changed the science of spectroscopy forever by introducing a breakthrough technology the S1000 Miniature Fiber Optic Spectrometer nearly a
43. he pointer becomes a crosshair symbol enabling you to drag the cursor around the graph Magnify Symbols When the magnify symbol is selected you can choose from among 6 magnify functions The function chosen will remain in use until another magnify icon or the crosshair symbol is selected Clockwise beginning with the top left symbol the magnify icons perform the following functions magnifies a specific area by clicking and dragging a box around an area zooms in on the horizontal scale but the vertical scale remains the same zooms in on the vertical scale but the horizontal scale remains the same zooms in approximately one point vertical and horizontal click once or press continuously zooms out approximately one point vertical and horizontal click once or press continuously reverts to the last zoom function OG Be Oo ho Cursor Diamond To move the cursor left or right in small increments in the graph area click on the left and right sections of the move cursor diamond The top and bottom sections of the diamond will send the cursor to the next or previous channel in your system The CHEM2000 and CHEM2000 UV VIS are single channel systems However additional channels can be purchased at any time 11 Cursor Properties Krick 766 46 nm gei Tel In this bar you can label the cursor and monitor the cursor s X value and Y value To the right of the X and Y values of the cursor is a cursor selection button that allows you to
44. hours minutes and seconds The duration of your kinetics experiment cannot exceed 24 hours Enter a Preset Sampling Interval value to set the frequency of the data collected in a kinetics process Be sure to select hours minutes and seconds Data from a timed acquisition is stamped with a time that is accurate to 1 millisecond Under Wavelength enter the single wavelengths from which you wish to collect data You can collect data from up to 4 single wavelengths characterized as a b c and d If you want the data graphed from these single wavelengths or from the mathematical calculations described in the next step select the Display box to the right of your values In the boxes next to x and y you have the opportunity to perform calculations on the data collected from the single wavelengths you specified as a b c and d In the y box you can also use x which represents the calculation used in x Click OK to confirm the parameters and close the dialog box Click the Scan button to begin the kinetics experiment Make sure that Continuous is selected The top half of the graph displays a real time full wavelength spectrum The bottom half of the graph displays the data for the selected wavelengths and their derived arithmetic calculations Each data set is stored with a time stamp Click the Stop button to stop the experiment However if a Preset Duration time was selected the experiment will automatically stop after the designated
45. hs characterized as a b c and d Display If you want the data graphed from these single wavelengths or from the mathematical calculations of these wavelengths described below select the display box to the right of your values Mathematical Calculations In the boxes next to x and y you have the opportunity to perform calculations on the data collected from the single wavelengths you specified as a b c and d Also in the box next to y you can use x which represents the calculation used in the box next to x Configure Hardware Hardware Configuration Ocean Optics Windows Device Driver 32 bit This command opens the Configure Hardware Version 3 01 00 dialog box It allows you to configure the specific type of hardware connecting the spectrometer to Spectrometer Type el the computer For the CHEM2000 and 52000 Pc2000 S Pixel CHEM2000 UV VIS systems you only need to A D Converter Type 50 select from Spectrometer A D Converter Type L cmgeecmmg Base Address I O Range and IRQ Interrupt Request The remaining hardware parameters are Base Address I 0 Range IRG Interrupt Request for other Ocean Optics systems The parameters in res 00300 this dialog box are usually set only once when Serial Port OOIChem is first installed and the software first starts There is no reason to change these parameters unless you need to alter the IRQ or SADS00 Pixel Resolution Base Address values See pag
46. iber Included as part of the CHEM2000 is a 400 um diameter single strand UV VIS optical fiber that couples easily via SMA terminations to the PC2000 and light source sample holder This silica core silica clad fiber is 2 meters in length For the CHEM2000 UV VIS the included optical fiber is a 300 um diamter solarization resistant patch cord that carries light from the sample to the spectrometer O0IChem Spectrometer Operating Software OOIChem is our standard Windows operating software for the CHEM2000 and CHEM2000 UV VIS systems It is a basic acquisition and display software that provides a real time interface to a variety of spectral processing functions OOIChem allows users to perform basic spectroscopic measurements such as absorbance transmission relative irradiance and concentration OOIChem operates with Windows 95 Windows 98 and Windows NT Visit our web site at www OceanOptics com Software_Updates asp to download free OOIChem upgrades OOIBase32 Spectrometer Operating Software OOIBase32 is our standard spectrometer operating software that we provide free of charge to all customers While OOIChem is a basic acquisition and display program OOIBase32 is user customizable and a much more advanced acquisition and display program With OOIBase32 you have the ability to control all system parameters collect data from up to 8 spectrometer channels simultaneously and display the results in a single spectral window perform reference moni
47. ics Windows Device Driver 32 bit ADC1000 PC2000 Version 3 01 00 3 Under Base Address choose the same setting as the dip switches on the A D converter and the Spectrometer Type S2000BT same available setting you found in your Js2000 Pc2000 x Giel Computer A D Converter Type 950 Jab C1000 PC2000 m Remember that the Input output Range Base Address you selected was expressed in hexadecimal Prett ec EA In this dialog box the base address is given in 768 0x0300 decimal followed by the hexadecimal equivalent in SC parenthesis For example 768 0x0300 gives the base address as 768 decimal and 300 hexadecimal fi al SAD500 Pixel Resolution 4 Under IRQ choose the same setting as the dip 1 switches on the A D converter and the same available setting you found in your computer aen 5 Only these parameters apply to your system Ignore the other settings they apply to other A D converters Click OK You can always change these settings once OOIChem is fully operational by selecting Spectrometer Hardware Configuration from the menu If you do not see the Configure Hardware screen exit the software Then select Start Run and type c windows ooidrv ini for Windows 95 98 systems or ec winnt ooidrv ini for Windows NT systems Notepad will open Edit this file for our device driver by finding the Tnitialized entry and making sure this line reads Initialized 0 Save the OOIDRV INI file and
48. ion from the menu e Coefficients From the Wavelength Calibration Report that came with your system enter the coefficients for your CHEM2000 or CHEM2000 UV VIS under Master e Trigger mode Select No External Trigger unless you have wired an external triggering device to the spectrometer for synchronizing data with an external event e Graph and chart display mode Choose Spectrum Only to only view live spectra from one spectrometer channel Choose Spectrum amp Kinetics to view both real time live spectra in the top half of the graph and to view a chart displaying your kinetics experiment in the bottom half of the graph e Flash Delay This function is for use with a strobe light source The CHEM2000 and CHEM2000 UV VIS systems do not come with a strobe light source e Color Temperature Enter the color temperature of your reference light source used in relative irradiance measurements For CHEM2000 users your lamp has a color temperature of 3100 Kelvin For CHEM20000UV VIS users your light source does not have a known color temperature Acquisition Parameters Set data acquisition parameters by choosing an integration period and selecting averaging and boxcar smoothing values Text Box Enter the operator name or any other identifying text here This text appears in your data files You can edit this text at any time Step 4 Connect Sampling Optics If you have a CHEM2000 take your 400 um fiber that came with your system screw o
49. it snugly into the holder 1 Locate the two ball plunger screws 2 Use the screwdriver to loosen the two ball plunger screws until the ball end of each screw is just visible in the holder 3 Insert your cuvette into the holder 4 Gently tighten the screws until the ball contacts the cuvette and starts to compress Do not over tighten Turning On the ISS 2 Attach the 400 um optical fiber that came with you CHEM2000 to the SMA connector on the front of the sample holder and attach the other end of this fiber to the SMA connector on the PC2000 installed in your computer 2 Plug the wall transformer into a standard 110 V outlet Plug the 12 V output into the back of your ISS 2 For users of European version wall transformers plug the transformer into a standard 220 V outlet 3 Find the on off switch on the back of the ISS 2 Turn the lamp on 4 Allow 30 minutes to stabilize before using Using the Filter Slots in the ISS 2 The filter slots one at the light source a second in the sample holder can be used to hold filters or light blocks The most useful filters include an FG 3 blue filter for increasing the relative energy near 400 nm and 800 nm compared to 600 nm an IR cutoff filter to reduce stray light below 750 nm and a 550 nm high pass filter to eliminate second order effects on Shortwave NIR measurements 1 To install a filter at the light source simply insert the filter into the filter slot The slot accommodates
50. l Hex320 0x320 Switch 1 2 3 4 5 6 ON OFF Value as shown 32 256 512 Example 816 decimal Hex330 0x330 Switch 1 2 3 4 5 6 ON OFF Value as shown 16 32 256 512 34 Interrupt Request Settings To change the IRQ settings on the PC2000 see the bank of switches on the A D converter The IRQ may be changed via the last 3 switches The following matrix defines the different IRQ settings by switch positions 7 8 and 9 In the default setting the IRQ is set to 7 Other combinations for IRQ settings are below After you have changed the switches reinstall the card and change the software settings to match the hardware settings See pages 6 8 for instructions The gray block indicates the position of the switch FE Switch is in the on upward position IRQ 7 Default Setting Switch 7 8 9 ON OFF IRQ 3 Switch 7 8 9 ON OFF IRQ 4 Switch 7 8 9 ON OFF IRQ 5 Switch 7 8 9 ON OFF IRQ 9 Switch 7 8 9 ON OFF IRQ 10 Switch 7 8 9 ON OFF IRQ 11 Switch 7 8 9 ON OFF 35 Appendix B PC2000 Pin outs and Jumpers It is not necessary for the average user to worry about the interconnect scheme as the cables supplied with all of the units need only be plugged into the matching connectors on the hardware However if the need arises to design and fabricate your own cabling system
51. l Though the standard CHEM2000 and Slave 1 CHEM200 UV VIS are single spectrometer channel systems you can Slave 2 purchase additional channels at any time For now select Master Slave 3 Slave 4 Slave 5 Slave 6 M ode of Operation Scope The signal graphed in Scope Mode is the raw voltage coming out of the A D converter Once you open OOIChem and it begins to acquire data you see the raw voltage from the detector expressed in A D counts This spectral view mode is unique to Ocean Optics It allows you to control signal processing functions before taking absorbance transmission and relative irradiance measurements Be careful when using this mode as it represents a combination of several factors the intensity of the light source the reflectivity of the grating and the Mode of Operation mirrors in the spectrometer the transmission of the fibers the response Slave 7 of the detector and the spectral characteristics of the sample Absorbance Transmission Scope Mode should primarily be used when selecting signal Relative Irradiance acquisition parameters such as integration period averaging and boxcar smoothing and when taking dark and reference scans Concentration Absorbance Selecting this mode switches the spectral window into Absorbance Mode Before this can occur both a dark and reference scan must be stored in Scope Mode See the Experiment Tutorial beginning on page 27 Absorbance is calcu
52. lated by the following equation When this equation is evaluated for each pixel of the detector the absorbance spectrum is produced S D Ax logio ks D a Di where Sis the sample intensity at wavelength A Dis the dark intensity at wavelength A Ris the reference intensity at wavelength A Transmission Selecting this mode switches the spectral window into Transmission Mode This is also the spectral processing mode used for Reflection spectroscopy as the math necessary to compute reflection is identical to transmission Before this can occur both a dark and reference scan must be stored in Scope Mode See the Experiment Tutorial beginning on page 27 The transmission of a solution is calculated by the following equation S D GT x 100 R D where Sis the sample intensity at wavelength A D is the dark intensity at wavelength A R is the reference intensity at wavelength A Relative Irradiance Selecting this mode switches the spectral window into Relative Irradiance Mode The reference spectrum must be made in Scope Mode with a blackbody of known color temperature CHEM2000 UV VIS users cannot make relative irradiance measurements because the light source that comes with the system is not a blackbody source with a known color temperature A dark spectrum is usually obtained by preventing light from entering the fiber that connects to the spectrometer See the Experiment Tutorial beginning on page 27 Relative irradianc
53. mation that is passed to a computer Finally the software performs basic acquisition and display functions on your data PC2000 PC Plug in Fiber Optic Spectrometer The PC2000 is 2048 element linear CCD array fiber optic spectrometer mounted on a 1 MHz A D card that fits easily into an ISA bus slot in the PC The spectrometer has a 25 um slit and provides a resolution of 1 5 nm FWHM The 12 bit 8 channel A D card is single ended and half length Though the standard CHEM2000 and CHEM2000 UV VIS are single channel systems additional slave spectrometer channels can be purchased at any time to expand wavelength range or perform multiple tasks Light Source Sample Holder The ISS 2 Integrated Sampling System that comes with the CHEM2000 is a versatile light source sample holder with a tungsten halogen bulb a built in filter slot for color correcting and other uses and a sample holder for 1 cm square cuvettes The light source sample holder is SMA terminated for easy coupling to optical fibers and has a 5 mm diameter f 2 collimating lens The ISS UV VIS Integrated Sampling System comes with the CHEM2000 UV VIS This light source sample holder has deuterium and tungsten bulbs and a cuvette holder for 1 cm cuvettes It occupies 7 1 2 x 4 and weighs lt 1 Ib The cuvette holder attaches directly to the light source and has a 5 mm diameter f 2 collimating lens to collect the light and funnel it to the solarization resistant optical fiber Optical F
54. n which is the formation of color centers or silica molecules with an unbound pair of electrons These color centers have an absorption band that is centered at 215 nm In regular HOH fibers our standard fibers the concentration of these color centers grows until nearly all UV light in the 215 nm region is blocked Other solarization resistant fibers are hydrogen loaded but their solarization resistant properties do not last as the hydrogen eventually leaks out of the silica meaning that these hydrogen doped solarization resistant fibers have a shelf life With Ocean Optics solarization resistant optical fibers there is no shelf life Care and Use Guidelines When fibers break they stop transmitting light Inspect fibers by eye to determine if light is being transmitted Bending the fiber will cause attenuation To minimize this effect add extra strain relief to both ends of the fiber Do not exceed the temperature specifications for the materials involved 200 C for the fiber 100 C for PVC cabling 100 C for standard epoxy Do not allow the fiber to be bent at a sharp angle A bending radius of less than 1 is dangerous Keep connectors and probe tips covered when the fibers are not being used Clean the ends of the fibers with lens paper and distilled water alcohol or acetone Avoid scratching the surface Do not immerse fiber ends in caustic materials or other solutions that can damage glass SPS o oe P FF
55. n back order Wavelength Calibration Report In your shipment box you will find your spectrometer in a silver gray anti static bag Your software diskettes and Wavelength Calibration Report are packaged with this manual A Wavelength Calibration Report comes with every spectrometer channel contains important information specific to your spectrometer and should be retained Use the calibration coefficients and intercept numbers on your Wavelength Calibration Report to calibrate the wavelength of your spectrometer U pgrades Customers sometimes find that they need Ocean Optics to make a change or an upgrade to their system In order for Ocean Optics to make these changes the customer must first contact us to obtain a Return Merchandise Authorization RMA number and to receive specific instructions when returning a product Quick Start The CHEM2000 and CHEM2000 UV VIS systems are easy to set up allowing the user to start collecting data within minutes This Quick Start provides brief instructions on installing your PC2000 installing and configuring the software and setting up your system To find detailed directions on a specific component see the Table of Contents Detailed PC2000 installation instructions begin on page 6 Detailed OOIChem software instructions begin on page 9 Step 1 Interface the PC2000 to your PC The PC2000 is our fiber optic spectrometer mounted onto an A D converter The entire instrument is installed into you
56. n your computer it has to be assigned a Base Address setting and an IRQ setting The default settings for each are Base Address I O Range 768 decimal 300 hexadecimal IRQ Interrupt Request 07 These default values are set on the A D converter There are dip switches on the A D converter and their positions determine the values These default values are set in the operating software as well Most of the time these default settings will work with your computer However if you have many devices installed in your computer you may have a conflict other devices may be using these settings If there is a conflict with another device in your computer you must change the positions of the switches on the A D converter For the PC2000 there is only one bank of switches on the A D converter the Base Address may be changed via the first 6 switches and the IRQ may be changed via the last 3 switches To first check your computer to see which settings are available follow the instructions for the Windows system that you use Windows 95 and Windows 98 Users Find Available Base Address and IRQ Settings Koch Go to Start Settings Control Panel and double click on the System icon 2 Choose the Device Manager tab and double click on Computer at the top of the list of devices in your computer 3 Under View Resources find available settings numbers unassigned to hardware Note these available settings for both the Interrupt request IR
57. ne end into the SMA connector on the light source and screw the other end of the fiber into SMA connector on your PC2000 which you have already installed in your computer If you have a CHEM2000 UV VIS take your 300 um solarization resistant fiber that came with your system screw one end into the SMA connector on the light source and screw the other end of the fiber into SMA connector on your PC2000 Step 5 Receive Data Run OOIChem in Scope Mode and take a reference spectrum and a dark spectrum see the Experiment Tutorial section beginning on page 27 for details Choose the absorbance transmission or relative irradiance mode to take your sample measurements Installing the PC2000 and OOIChem Software The PC2000 PC PLUG IN SPECTROMETER has our 2048 element linear CCD array fiber optic spectrometer mounted onto an ADC1000 A D card The ADC1000 A D CARD is a high speed ISA bus analog to digital converter card that connects our miniature fiber optic spectrometers to desktop PCs The ADC1000 is a 12 bit 8 channel single ended A D card This sturdy spectrometer A D card combination fits easily into an ISA bus slot in the PC The following directions apply to both CHEM2000 and CHEM2000 UV VIS users Each device in or connected to your computer is assigned specific settings it s similar to giving each device its own name so that your computer will know what to call and how to recognize the device In order for your PC2000 to work as a device i
58. ngsten bulb Bulb Replacement The deuterium and tungsten bulbs cannot be replaced by unauthorized personal To replace a bulb in the ISS UV VIS contact Ocean Optics 24 Specifications Deuterium wavelength range 200 400 nm Tungsten wavelength range 360 850 nm Deuterium bulb life 800 hours Tungsten bulb life 2 000 hours Ignition delay 1 5 seconds to 60 seconds Peak to peak stability 0 3 in 30 minutes 1 0 in 10 minutes Connector SMA 905 Power input 12V Power requirement 12 VDC 420 mA Power consumption 5 watts deuterium 3 8 watts tungsten 1 2 watts 25 300 um Diameter Solarization resistant Optical Fiber The 300 um Diameter Solarization resistant Optical Fiber that comes with your CHEM2000 UV VIS is a single strand optical fiber The active part of our solarization resistant fibers consists of a silica core surrounded by a silica cladding material The fiber is then coated in aluminum Our standard laboratory cabling is blue PVC Our solarization resistant fibers are best used for applications where exposure to long term UV light occurs Solarization is the loss of transparency in glass due to exposure to ultraviolet radiation If you are using a UV light source the UV radiation degrades the silica in a standard patch cord fiber over time resulting in increased overall absorption values and invalid data This degradation is called solarizatio
59. older Enter the known concentration of the standard solution in the chart in the Concentration column next to Solution 1 31 8 10 11 12 13 14 15 16 17 18 19 Click the Scan button or select Scan Solution from the menu The absorbance value will appear next to the concentration for Solution 1 At any point you can select Edit Clear from the menu to clear the dialog box of all data Calculate ab sp Dune LS tarvebength masted 8 D Jee For aac alu ent the korn concentration value and cick rent When dons chok yew L sd esaann Lara Solution el lanes i Abiba Lats Callbralion Cursi RES BERR Pa Mi DH 2 au Sean i D i EH L za Fand ee _ Fila Pai i Be L7 a 38 _ LE 10N Bee 17m er a5 er SS T a UWE SE TE ee en en ee ee m em SEAN Loes iret I Take Solution 1 out of the cuvette holder and put in another standard solution with the next highest known concentration Enter the known concentration of the standard solution in the chart in the Concentration column next to Solution 2 Click the Scan button or select Scan Solution from the menu The absorbance value will appear next to the concentration for Solution 2 You may continue to scan solutions with known concentrations You must scan at least 2 in order to achieve a calibration curve When you have completed taking scans of your solutions with known concentrati
60. olutions we offer a transmission dip probe with screw on removable tips in 2 mm 5 mm or 10 mm path lengths Contact Ocean Optics for more information To take a transmission measurement 1 Select Scope under Mode of Operation in the software display area Make sure the signal is on scale Adjust acquisition parameters so that the peak intensity of the reference signal is about 3500 counts Take a reference spectrum by first making sure nothing is blocking the light path going to your spectrometer The analyte you want to measure must be absent while taking a reference spectrum Take the reference reading by clicking the Reference button in the software display area This command merely stores a reference spectrum To save a spectrum you must select File Save Spectral Values from the menu Storing a reference spectrum is requisite before the software can calculate transmission spectra While still in Scope Mode take a dark spectrum by first completely blocking the light path going to your spectrometer If possible do not turn off the light source If you must turn off your light source to store a dark spectrum make sure to allow enough time for the lamp to warm up before continuing your experiment Take the dark reading by clicking the Dark button in the software display area This command merely stores a dark spectrum To save a spectrum you must select File Save Spectral Values from the menu Storing a dark spectrum is requisite be
61. ons click the View Calibration Curve button You will then have the Intercept and Slope of your curve The Slope is the necessary to compute Beer s Law and to find the unknown concentration of a solution At this time you may also select a label for your concentration values such at Moles per Liter in the Concentration Units box This is only a label and does not affect the data in any way Select Edit Show Legend from the menu to display the legend for the calibration curve The legend allows you to choose a plot design point style line style line width and plot color Select Edit Show Palette from the menu to display a variety of options for configuring the curve The palette provides features such as autoscaling graph formatting value precision mode mapping and graph positioning You can select File Print from the menu for the dialog box to print the dialog box To save the current calibration curve data select File Save from the menu for the dialog box Select File Close from the menu for the dialog box to close this dialog box and return to the main window When a message box asks if you would like to use this calibration curve when calculating concentration values select Yes Now that you are back in the main display window place the solution with the unknown concentration of a substance in the cuvette holder Under Mode of Operation select Concentration Click the Scan button to receive your concentration values
62. own concentration and put it in the cuvette holder Make sure nothing is blocking the light going to your sample Then select Absorbance under Mode of Operation Click on the Scan button in the display area to take a scan Make sure Single is selected To save the spectrum select File Save Spectral Values from the menu 4 Now select the wavelength for monitoring the concentration of your solutions by choosing Spectrometer Select Concentration Wavelength from the menu Move the cursor to the highest absorbance peak of the spectrum of the solution with the highest known concentration and choose Select Move cursor to desired wavelength and click 5 Remove the solution with the highest known concentration Select Spectrometer Calculate a Calibration Curve from the menu The Calculate Calibration Curve dialog box opens Now you will begin taking scans of the rest of your series of standard solutions with known concentrations from the lowest known concentration to highest all while working in this dialog box 6 If you wish you can take a new reference and a new dark scan for each solution by choosing Scan Dark and Scan Reference from the menu of this dialog box However in this case it is not necessary If no reference or dark scan is taken at this point the software will use the reference and dark scans taken in Steps 1 and 2 to calculate absorbance 7 Take the solution with the lowest known concentration and put it in the cuvette h
63. r PC But first you must find available Base Address and IRQ values 1 The default settings for our A D products are a Base Address or Input Output Range of 768 decimal and an IRQ of 7 You will need to match Base Address and IRQ settings on the A D card to available settings in your computer First determine which settings are not being used by other hardware devices e Ifyou have Windows 95 98 go to Start Settings Control Panel Double click the System icon Choose the Device Manager tab and double click on Computer at the top of the list of devices Under View Resources note available settings numbers unassigned to hardware Remember that these I O settings are expressed in hexadecimal and correspond to our Base Address which is given in decimal followed by the hexadecimal equivalent in parenthesis e Ifyou have Windows NT go to Start Programs Administrative Tools Common Windows NT Diagnostics Click on the Resources tab Select the IRQ button Find an available IRQ Select the I O Port button Find an available I O Range Base Address 2 Note these available settings When you first run OOIChem you must enter these values in the Configure Hardware dialog box 3 Turn off the computer and take off the computer cover Ground yourself to the computer chassis or power supply and remove the PC2000 spectrometer A D combination from its static shielded bag 4 If necessary change the position of the switches on the A D
64. rew on the bottom of the lamp to hold the bulb in place 10 Close the two halves of the lamp being careful not to pinch the wires 11 Replace the two screws at the back of the lamp and secure the fan to the bottom of the lamp Spectral Output of the ISS 2 This graph represents a blackbody curve for the 3100 K color temperature tungsten halogen light source The actual spectral output of the lamp will vary due to the spectrometer configuration grating efficiency the sampling optics used and the detector s efficiency 1 Normalized Intensity o gt bt Q N fi 300 500 700 900 1100 1300 1500 1700 1900 Wavelength nm 900 hour bulb 3100K 20 1SS 2 Specifications Path length 1 cm Collimating lens BK 7 glass 360 nm 2 um 5 mm diameter f 2 Collimating lens termination SMA 905 Filter slot accepts filters up to 1 4 6 mm in thickness Base material aluminum Spectral range 360 nm 2 um 9 0 cm x 5 0 cm x 3 2 cm LWH Dimensions 3 5 x 2 0 x 1 25 LWH Power input 12 VDC 800 mA 7 20 VDC 0 5 2 amps Power output 6 5 watts Bulb life 900 hours Bulb color temperature 3100K Output to bulb 5 volts 1 3 amps Output regulation 0 2 voltage Time to stabilized output 30 minutes The useable range of the ISS 2 is limited to the wavelength range of the spectrometer to whic
65. s Scientist for more information First Second and Third Coefficients From the Wavelength Calibration Report that came with your system enter the First Coefficient Second Coefficient and Third Coefficient under the Master column Though the CHEM2000 and CHEM2000 UV VIS come with only a Master spectrometer channel additional spectrometer channels can be added om L Ces at any time meat SE Trigger Mode With the CHEM2000 or CHEM2000 UV VIS and OOIChem Operating Software you have two methods of acquiring data In the No External Trigger Mode or Normal Mode the spectrometer is free running That is the spectrometer is continuously scanning acquiring and transferring data to your computer according to parameters set in the software In this mode however there is no way to synchronize the scanning acquiring and transferring of data with an external event To synchronize data acquisition with an external event the External Software Trigger Mode is available It involves connecting an external triggering device to the spectrometer and then applying an external trigger to the spectrometer before the software receives the data In this mode the spectrometer is free running just as it is in the Normal Mode The spectrometer is continually scanning and collecting data With each trigger the data collected during the integration period is transferred to the software All acquisition parameters such as th
66. t page In the first column place the exact or true wavelength of the spectral lines that you used Most calibration line sources come with a wavelength calibration sheet If you do not have a wavelength calibration sheet for your light source you can probably find the wavelengths for your spectral lines if they are being produced by pure elements in the first column of the worksheet In the second column of this worksheet place the observed pixel number In the third column calculate the pixel number squared and in the fourth column calculate the pixel number cubed 37 Independent Dependent Values computed from Variable Variables the regression output True f Predicted 2 3 We Pixel Pixel Pixel Wavelength Difference 253 65 15 30625 5359375 253 56 0 09 296 73 296 87616 25934336 296 72 0 01 302 15 312 97344 30371328 302 40 0 25 313 16 342 116964 40001688 313 02 0 13 334 15 402 161604 64964808 334 19 0 05 365 02 490 240100 117649000 365 05 0 04 404 66 604 364816 220348864 404 67 0 01 407 78 613 375769 230346397 407 78 0 00 435 84 694 481636 334255384 435 65 0 19 546 07 1022 1044484 1067462648 546 13 0 06 576 96 1116 1245456 1389928896 577 05 0 09 579 07 1122 1258884 1412467848 57901 0 06 696 44 1491 2223081 3314613771 696 70 0 15 706 72 1523 2319529 3532642667 706 62 0 10 727 29 1590 2528100 4019679000 727 24 0 06 738 40 1627 2647129 4306878883 738 53 0 13 75147 1669 2785561 4649101309 75127 0 19
67. that comes with the system is not a blackbody source with a known color temperature Kinetics Configuration Select Spectrometer Kinetics Configuration from the menu to configure and establish the parameters for a kinetics experiment In the Kinetics Configuration dialog box you can collect spectral data as a function of time from up to 4 single wavelengths and up to two mathematical combinations of these wavelengths Data from a kinetics experiment will not be displayed in the graph unless you choose Spectrometer Spectrometer Configuration from the menu and select Spectrum amp Kinetics next to Graph and chart display mode This way not only will your kinetics experiment be displayed in the bottom half of the graph area you will also still see real time spectra in the top half of the graph area Preset Duration Kinetics Condequnalice Enter a value to set the length of time for the entire kinetics DH SS ek Dipha process Be sure to select hours E Presei Daten sl 600 Im EI minutes and seconds Your b _ ep we ol kinetics experiment cannot Peng Samping Ier e a00 e i d 300 exceed a duration of 24 hours ziik rm LI E Preset Sampling Interval bein Enter a value to set the frequency of the data collected in a kinetics y D Ewe bi process Be sure to select hours minutes and seconds Wavelength Enter the single wavelengths from which you wish to collect data You can collect data from up to 4 single wavelengt
68. the ISS UV VIS through our more advanced OOIBase32 Spectrometer Operating software select or deselect the Strobe Enable box in the Acquisition Parameter dialog bar above the graph area to turn the light source in the ISS UV VIS on and off Refer to the OOIBase32 Spectrometer Operating Software Manual for more details 4 There can be up to a 1 5 second delay between turning the bulbs on via the software and the bulbs igniting If the lamp has not been used recently the deuterium bulb may take up to 60 seconds to ignite 5 For 0 3 peak to peak stability allow 30 minutes warm up time before taking your measurements Disabling the Tungsten or Deuterium Bulb It is possible to disable the deuterium or the tungsten bulb in the ISS UV VIS Both bulbs are enabled at the time of manufacture In order to disable the deuterium or tungsten bulb you must remove the casing of the ISS UV VIS Disabling the Deuterium Bulb Jumper block JA of the ISS UV VIS s circuit board controls the deuterium bulb Short pins 2 3 that is place a jumper over pins 2 3 of JA to enable the deuterium bulb Short the pins 1 2 that is place a jumper over pins 1 2 of JA to disable the deuterium bulb Disabling the Tungsten Bulb Jumper block JB of the ISS UV VIS s circuit board controls the tungsten bulb Short pins 2 3 that is place a jumper over pins 2 3 of JB to enable the tungsten bulb Short pins 1 2 that is place a jumper over pins 1 2 of JB to disable the tu
69. toring and time acquisition experiments and use numerous editing viewing and spectral processing functions At any time users can receive free OOIBase32 updates from our web site at www OceanOptics com Software_Updates asp These are the standard components for the CHEM2000 and CHEM2000 UV VIS systems However we offer a complete line of light sources sampling holders in line filter holders flow cells and other sampling devices an extensive line of optical fibers and probes and collimating lenses attenuators diffuse reflectance standards and integrating spheres All components have SMA terminations so that changing the sampling system is as easy as unscrewing a connector and adding a new component or accessory This modular approach components are easily mixed and matched offers remarkable applications flexibility Users pick and choose from hundreds of products to create distinctive systems for an almost endless variety of optical sensing applications Packing List A packing list comes with each order It is located inside a plastic bag attached to the outside of the shipment box The invoice is mailed separately The items listed on your packing slip include all of the components in your order However some items on your packing list are actually items installed into your spectrometer such as the grating and slit The packing list also includes important information such as the shipping address billing address and components o
70. ulate Calibration Cume nenn nna 16 Enable Str be u 2 atana aa an aeaa a an a Raan d a O aaa LAE ea Aea EN auaa agement 16 Spectrometer Configuration cccccceeccecseeceeeeeeeeeeceeeeecaaeeeeaaesseeeeseaeeesaaesseaeesseeeescaeesenaeeesaes 16 Kinetics Configuration deceit 2 sie ISCH In 18 Hardware Contigurationas scatters ean eeh ve dee ee 18 CHEM2000 Sampling Components eeeneeeenneennnnn 19 ISS 2 Integrated Sampling System ennennnnnennnnnnnnnennnnnn 19 400 um Diameter Optical Fiber oo reese 22 CHEM2000 UV VIS Sampling Components eee tees eeteeeeteteteeneeeees 23 ISS UV VIS Integrated Sampling System nennn 23 300 um Solarization resistant Optical Fiber nneeenenn 26 Experiment THEN Eeer 27 Absorbance Esperlenbe tee 27 Transmission Experiments ccc cesccecesesseecsscsecsesececsacsecsscsecsaceecsusasesesaseecsaes 28 Reflection Experiments s na einen 29 Relative Irradiance Experiments uennennnnnnennnnnnnnnnnnnnnnnn 30 Concentration Experiments ccccccceccceceececeeeeceeeeceeeaeeeeecaecaeeeseseecaeeeseeeeeaeeesaeeeeaeers 31 Kinetics Experiments sa nase 33 Appendix A Changing the Settings on the PC2000 34 Appendix B PC2000 Pin outs and Jumpers cece cece ee eeeteeeeeeeeeeneee 36 Appendix C Calibrating the Wavelengt

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