Software User Guide V 2.2.1
Contents
1. 29 Number of Readings Total Downloaded 1499 1724 Clear Memory f 2 pra pe B I SEAS UNE a _ cr A pre J T E a gi agay im A Status Search g Scan with AVG Free Properties Open All Users Explore All Users fam 2 Windows Explorer 14 Paint f BolusID Notepad Sag Num Lock FES File Edit wiew Favorites Tools Help ae Back gt wi rs Search Key Folders iss Address C bolus Data Si Go Folders demo01_B_0029_06072010_XXx Aa C5 File ej Desktop 75 KB Open B My Documents Edit E 3 ae maou g Scan with AVG Free pa aig Open with gt O bolus Data Compressed zipped Folder l Uploads Cut E Desktop create shortcut H O CMPNENTS mi Config Msi Copy LJ Mail Recipient 5 d132e7ecdafa073d03fc Create Shortcut B My Documents E O Documents and Settings Delete O gt ECOWBACKUP D 5 Administrator Rename E O All Users maai 3 DEMon1 gd O Desktop E D Favorites E O My Documents eCow Ltd Unit 5b King Street Business Centre 7 9 King Street Exeter UK Version 2 2 3 January 2013 LIMITED Electronic Cow Management eCow 4 Adding More Boluses The system was set up with bolus identities specific to your site To add boluses you need to either replace the file C bolus BolusID DAT with one we send you or edit the file using Notepad or another text editor To edit the BolusID DAT file browse2. 30 00 DI TJA BS A 02 02 Be 5 0 P09 06 1 a 00 73 20 30 00 00 73 04 5F 02 A 9 02 C4 10 00 04 75 C D 4 7 00 08 06 1 1 00 73 20 40 00 00 7304 SF 02 4A9 02 04 10 00 04 76 0 47 00 03 06 11 00 73 20 30 00 O0 73 0A 5F02 49 02404 10 00 04 76 0D 47 00 08 06 11 hl Messages out Success Errors Warnings 5867 es car 7 Current Bolus Data Clock WatchDog Temperature Radio Address Current Logging Data Date Time pH Redox Temp Battery Mode Status WD No readings Sie aaua onzas SS ne ran orotate EEA n ea Siow Modo Fast Modo Test Channels Take a new reading 7 Battery Voltages Our eBoluses are shipped with a starting voltage of at least 3 00v in the logged data At 2 70v and below the pH amplification looses accuracy However our bench tests have shown this means the accuracy is only lowered to 0 4pH as opposed to 0 20H when newly dispatched If this is acceptable for your application the device can keep logging until the logged voltage is as low at 2 05v Battery Voltage a 24 hour 10 15 Weeks remaining eCow Ltd Unit 5b King Street Business Centre 7 9 King Street Exeter UK Version 2 2 3 January 2013 8 Deployment When deployed into the cow using a balling gun by a qualified technician you can ensure optimal download by finding the sweet spot on the cow Allow the eBolus to settle for at least 1 hour before trying to make conta
3. IMITED Electronic Cow Management eCow 6 Calibrating the Redox Sensor if enabled To calibrate the Redox sensor select Redox from the Calibration Type menu Calibrate Redox Program Calibration Type Reference Redox ADC Average XX Low Point_ 1376 0 XX High Point 129 0 Abort Command offset 4103 Full Calibration Gain 801 Status Exit Calibration Our software sets up a two point calibration that enables the software to know the slope and gain for each of the sensors However many authorities only use a Single point calibration or standardisation for redox on theoretical grounds To do this select the test form and press latest reading and you will see the Redox value in the relevant box Do this in a known Redox solution for a single point standardisation or if you have two solutions go to the calibrate bolus page and follow the procedure similar to pH You will have to enter your Redox standard values into the jow and high points as these will differ between manufacturer and temperature Follow the specification of your buffer for the value to enter here eCow Ltd Unit 5b King Street Business Centre 7 9 King Street Exeter UK Version 2 2 3 January 2013 Electronic Cow Management LIMITED eLow stForm Program Messages Transmit Clear Cancel Transmit Transmit Receive RSSI dBm 00 73 20 30 00 A 00 73 04 89 02 49 02003 10 2B04 75 0C 47 00 09 06 16 ima OD 3 20
4. LIMITED Electronic Cow Management eCow eBolus Software User Guide V 2 2 2 January 2013 eCow Ltd Unit 5b King Street Business Centre 7 9 King Street Exeter UK Version 2 2 3 January 2013 Electronic Cow Management LIMITED eCow Contents OS TNS A E cesses E tect eons E T 2 PPG A APO BOE EUO e E E A E E EE EE EEE 3 Device storage amd Main te Mate ssisecyiniri e iEn I AER 3 1 Plug in Dongle and Switch on the Computter cccccssesssssssssssssssseeeeeeeeeees 4 Dis NOW CO AO D e E A TA A E E 4 s T Dae E E E 6 A A ddp More Boli E eaea a E a a di 7 5 Calibrating the Sensol ccccccccssssssssssesssssseesesssssesseesssesssssssssseeeeeceeeasaeeeeeess 7 6 Calibrating the Redox Sensor if enabled ccecceeeeeeeeseeeeeeeeeeeeeeeeeaas 9 Ty NPAC BY VOM ASS ceca hep eh Sreeedona E E E E E tn EE 10 ge MS Ly E daa E E Set cs ba A case aoe tee ieee 11 9 Troubleshooting and things to remember when calibrating eeeeeeees 11 Download SUCCESS criteria oss svasconssncscrscassssnssnnntesdnatiaveascoeatnesseoreuesnaadaisbayeimeddoiaeees 20 Accuracy Success Criteria Quick teSt cccccccccsssssseseeeeeeeeeeeeeesaaeaeceeeseaeanees 20 Accuracy SUCCESS Criteria 2 formal ES bovcviccvescave tecerasecccvevecescerapcueseeeontcensspectens 20 eCow Ltd Unit 5b King Street Business Centre 7 9 King Street Exeter UK Version 2 2 3 January 2013 LIMITED Electronic Cow Management eCow Preparatio
5. ND SOLUTIONS PoC Aceticacid SMS Pp oCENA HCOn ET A Po NaHPOL12HO OO o 93g PO O GI KG I Po CaCl anhyd 4 gg Po MgCl anhyd 0B Fg PoC EN OH SN HCI MAIN STEPS ACTION NAME Using an analytical balance weight the following constituents NaHCO 9 80 g NazsHPO 12H2O 9 30 g NaCl 0 47 g KCI 0 57 g CaCl anhyd 0 04 g MgCl anhyd 0 06 o 2 Dissolve the CaClzin about 10 ml of deionised water before adding to the other constituents Dissolve the other constituents in 750 ml of deionised water using the magnetic mixer Add the 10 ml of CaCl solution Lo a a Add 20 ml of 5M acetic acid Complete the volume to 1000 ml with deionised water 7 Final pH should be in the range of 5 5 to 6 5 If it is not adjust pH using either HCI 5N or NaOH 5N eCow Ltd Unit 5b King Street Business Centre 7 9 King Street Exeter UK Version 2 2 3 January 2013 LIMITED Electronic Cow Management eCow Appendix C Bolus acceptance protocol This is the protocol to be operated by the customer to demonstrate that the eBoluses are operating correctly Switch on the hand held eCow computer and login as the nominated user this is not password protected but can be at the customer s discretion This is a touch screen computer but it is not waterproof and will not operate in the rain Put it into a clear plastic bag if working in wet conditions The eCow software should start automatically a
6. adings Total Downloaded o 0 Clear Memory Status Checking for Bolus Select File __ CAbolus Data demo01_B_0029_06072010_xXx csv Exit Download eCow Ltd Unit 5b King Street Business Centre 7 9 King Street Exeter UK Version 2 2 3 January 2013 LIMITED Electronic Cow Management eCow First the message Checking for Bolus will appear followed by a number relating to the data points stored Previous Clear Memory Select File Exit Download lf the number of points downloaded does not equal the number of points stored then you should press Download and repeat until all the data is downloaded Examine the file in C bolus Data It will be named with your site name the bolus number and the date terminating in csv It can be opened with Notebook or in a spreadsheet Examine the last line it should be close to the current time Back this file up You can now press Clear Memory and the PC s time will be sent to the eBolus eCow Ltd Unit 5b King Street Business Centre 7 9 King Street Exeter UK Version 2 2 3 January 2013 Electronic Cow Management LIMITED eCow 3 Transfer Data Right click over the Start icon and select explore Select the Data folder from the bolus folder and copy to a USB stick Copy the files created onto a USB stick and email them to info ecow co uk This data can help determine when your eBolus needs replacing by analysing battery rate of depletion Bolus ID
7. awakened Be aware that to do a full calibration normally takes approx 30 minutes for each eBolus so do calibrations a day or two prior to deployment Once calibrated the device remains in calibration for months when kept hydrated As a new customer please become a registered user on our eCow website and email harry ecow co uk to upgrade you to customer status Then you can access our video tutorials http www ecow co uk customer support video tutorials and software guides Device Storage and Maintenance eBoluses should be stored in pH 4 Buffer for both overnight and long term storage Ensure they are kept below 30 C to retain maximum battery life Slow response and non reproducible measurements are signs that the electrodes have become coated or clogged If the device becomes coated or clogged the time taken to make a measurement will increase normally 95 of final readings should be achieved in less than 10 seconds Rinsing with methyl alcohol should remove the coating and restore the speed of response Redox electrodes may need additional cleaning from time to time with crocus paper If the methyl alcohol rinse does not restore the response soak in 0 1M Hcl for five minutes Remove and rinse with water and place in 0 1M NaOH for five eCow Ltd Unit 5b King Street Business Centre 7 9 King Street Exeter UK Version 2 2 3 January 2013 LIMITED Electronic Cow Management eCow minutes Remove rinse again and soak in pH 4 bu
8. ct Make sure the antenna is positioned beneath the cow in the eBolus area of residence ideally just behind its point of elbow and about 5cm away from its skin See below The larger the size of your cows the more difficult it may be to communicate with the eBoluses This is because larger animals tend to have more body mass and more dense bones It is important once you have made contact with the eBolus to not move the dongle as this line of sight contact is easily broken We would recommend asking for a latest reading first and once a message is successfully received and the eBolus is in fast mode THEN press download button 9 Troubleshooting and things to remember when calibrating When calibrating if you have the eBoluses in a cluster close together in parallel the dongle has limited line of sight to the eBolus in question and struggles to make contact The setup we use to talk to and calibrate our eBoluses is highlighted in the photos below where eBoluses are being pre warmed in the tub to the right on a heat plate with a thermometer inside the tub Then the eBolus to calibrate is stood vertically in pots filled with pH 4 and eCow Ltd Unit 5b King Street Business Centre 7 9 King Street Exeter UK Version 2 2 3 January 2013 Electronic Cow Management LIMITED eCow pH 7 Again another thermometer to ensure correct temperature is reached Ensure cleaning with de ionised water between buffers Also when placing the eB
9. e Temperature Check Interval E g If the Temperature Check Interval is set to 3 minutes and the Logging Time to 15 minutes then five readings will be taken 3 minutes apart and the average stored eCow Ltd Unit 5b King Street Business Centre 7 9 King Street Exeter UK Version 2 2 3 January 2013 Electronic Cow Management LIMITED eCow Radio Check Time Window To save power the eBolus can be programmed so it doesn t check the radio during the night when it s unlikely that the operator would need to download any data A check is made on the radio between the Start and End Times at the Radio Interval Note only Hours and minutes are used no seconds Bolus ID Idle Calibrate Logging Temperature Check Interval Minutes Radio Interval Slow Mode ae ie Radio Interval Fast Mode Seconds Logging Time Multiple of TCI Minutes Radio Check Time Window start Hrs Min Sec End Hrs Min Sec 00 00 00 23 59 00 Sane tintecd Press the Download Settings button to transfer the settings to the eBolus eCow Ltd Unit 5b King Street Business Centre 7 9 King Street Exeter UK Version 2 2 3 January 2013 LIMITED Electronic Cow Management eCow Appendix B Preparation of artificial rumen fluid Define the preparation of 1000 ml of artificial rumen fluid Preparation of artificial rumen fluid for conditioning pH boli EQUIPMENT DESCRIPTION P Analytical balance Magnetic stirrer po pH meter ft MATERIAL A
10. e of data downloaded within 1 hour of starting download Accuracy An approximate check on accuracy involves passing a rigid sample tube into the rumen via the fistula and drawing a sample from close to the end cap of the sensor and measuring it immediately Accuracy Success Criteria quick test The sample drawn from near the bolus end when compared to the immediate pH value will be within 0 2 pH units of that recorded by the comparative instrument Accuracy Success Criteria 2 formal test After the agreed period of measurement say 1 week for a new customer the eBolus should be removed rinsed in warm water and placed in a vessel of 4 pH buffer at 39 C On interrogation after 5 minutes the bolus output should be between 3 8 and 4 2 pH The process should be repeated in buffer 7 and after 5 minutes the bolus output should be between 6 8 and 7 2 pH After this the bolus can be recalibrated and placed in another cow If there is a difference you may want to apply a linear adjustment to the data file but this is at your own risk as the drift may not be linear eCow Ltd Unit 5b King Street Business Centre 7 9 King Street Exeter UK Version 2 2 3 January 2013
11. e set by selecting the appropriate tab eCow Ltd Unit 5b King Street Business Centre 7 9 King Street Exeter UK Version 2 2 3 January 2013 eCow LIMITED Electronic Cow Management Bolus ID Idle Calibrate Logging Idle The only parameter for idle mode is the Temperature Check Interval At this interval the eBolus switches on and checks the temperature to see if the mode has changed before returning to sleep No radio communication is possible in this mode Maximum time interval is 34 minutes The recommended interval is 5 minutes Calibrate Calibrate mode has a Temperature Check Interval used to check the temperature and change modes Unlike idle mode this is not used for a sleep time see below Radio Interval fast and slow modes sets the sleep time the longer the sleep time the longer battery life There are two modes fast and slow Normally it operates in slow mode because fast communication is not required If fast communication is required then the software switches to fast mode Recommended settings Bolus ID Idle Calibrate Logging Temperature Check Interval 1 Minutes Radio Interval Slow Mode 60 Seconds Radio Interval Fast Mode 10 Seconds Logging Temperature Check Interval Radio Intervals are the similar to those used for calibration mode Logging Time sets how often pH readings are stored The stored readings are an average taken since the last reading with each individual reading time being set by th
12. ffer for 10 minutes before use 1 Plug in Dongle and Switch on the Computer Plug in the USB Dongle before turning on the Computer Never unplug the dongle whilst the software is running as this will require a complete re installation of the software Ensure the PC time is correct for your country Change by right clicking on clock at the bottom right hand side the screen The computer has been set up to automatically load the pH eBolus software from login If this does not occur then double click on the pH eBolus icon on the desktop The software automatically searches and finds the USB Dongle and when successful a green light emits from the dongle po Hallie If the short cut doesn t work pH bolus will be found in C Program Files eCow Ltd pHBolus A record of all calibrations and configurations are sent to the eBolus and this is kept in C Bolus Uploads folder and the file is called Bolus followed by the ID number It is a CSV format file which will load directly into Excel A record of all downloads is also kept in the same file 2 Download Data As the eBoluses are set at 00 00 GMT you need to send each eBolus the newly determined time by doing a data download then clearing its memory This will send the PC time to the device Press the Download button on the Start Page Change the ID of the bolus to that within the cow Press Download the system will do everything until download has failed or succeeded Program e
13. n for Use All eBoluses are shipped with the sensing end covered with a hydrating blue vinyl cap Remove the tape and the blue vinyl cap from the metal end During shipment it is possible for air bubbles to move into the glass bulb of the sensor To remove the air shake down the device in the same manner as a clinical thermometer until the glass bulb is filled with solution You may need a magnifier and lamp to see this from the holes of the end cap After rinsing the sensing end with deionised water the eBolus must be warmed up All eBoluses have a responsive time window of 00 00 to 23 59 GMT but only log when above 35 C When interrogating an eBolus for the first time ensure it has been warmed for at least 20 minutes between 35 C and 45 C Use an independent thermometer to check the temperature of your heat block water bath The Q1 computer has been set to 00 00 Greenwich Mean Time Please change the Computer s time zone suitable to your country by right clicking on the clock at the bottom right of the screen Each bolus has been accurately calibrated for pH and or Redox using ORP Buffer Test Kit pH 4 265mV and pH 7 92mV which can be purchased from our online store http shop ecow co uk We recommend you buy ORP Buffer Solution and Electrode Test Kit so you can complete a calibration On each bolus there may be up to 5 data points stored these fabrication memories exist so you the customer knows when it was first
14. nd the green bar state Connected If this bar is red or says Not Connected check the USB port is connected to the antenna and try again Press the screen button that says Configure Bolus and also calibrate the eBolus and set the eBolus clock to computer time Insert the eBolus into the cow by fistula It will take up to 2 hours to migrate to its final resting place in the rumen reticulum The eBolus will rest approximately 100 mm inside the cow at a point approximately 300 mm tail wards from the front legs about 100 mm from the centreline of the cow A metal detector tuned to maximum range will be provided for demonstration purposes to aid in its location The software must be given the correct identity of the eBolus If set running it will continue working for 10 minutes before time out If data has not been downloaded repeat the process Most data will download within a few minutes of contact being established However breaks in transmission can occur and the process may need to be repeated pH sensors do drift principally due to different ionic status Accurate results will only be achieved if the sensor is recalibrated after being kept either in rumen liquor for 24 hours or preferably removed from the cow and calibrated at 39C eCow Ltd Unit 5b King Street Business Centre 7 9 King Street Exeter UK Version 2 2 3 January 2013 Electronic Cow Management LIMITED eCow Download success criteria A complete fil
15. olus in the buffer pots give them a sharp spin in the pot to push out any trapped air bubbles The hood to the heat block is wrapped in tinfoil as our office is noisy with conflicting radio waves and this simple measure helps a great deal to get eBoluses responding fast Notice both the dongle and eBolus are under the tinfoil hood eCow Ltd Unit 5b King Street Business Centre 7 9 King Street Exeter UK Version 2 2 3 January 2013 LIMITED Electronic Cow Management eCow Calibration and set up files relating to the eBolus reside in the C bolus directory under normal circumstances these are not needed by the user Data files are automatically labelled and stored in the C bolus data directory If the antenna is accidentally pulled out when the software is running the program cannot work until the antenna is reconnected and the computer is restarted Never dip the eBolus into your stock buffers decant a small amount from stock and assume that it will quickly become contaminated Rinse the sensors between tests with deionised water by washing the the water through the crossholes ideally with a squeezy bottle Redox solution can poison the pH electrode for several hours so do the pH calibration last Ideally immerse the end cap of the eBolus in rumen liquor or an analogue a recipe for this is in Appendix A to condition the pH probe for several days but keep the liquor below 30C to keep the eBolus inactive Redox p
16. otential is very sensitive to temperature so maintain the eBolus at a stable temperature of 35 C for calibration pH is less sensitive to temperature but our process ensures that the eBolus is in the correct zone The best guide to temperature is that given by the eBolus Go to the test form page and press the temperature button and you will see the current temperature this may differ from your bath setting because of heat loss from the surface On extraction from the cow the eBolus will be heavily contaminated and the cross holes may need to be swilled out of rumen debris The crossholes restrict the flow of buffer to the sensors so do move the eBolus about to ensure flow through Radio does not travel well through water or steel so do not fill your bath too deep and mount the antenna in line of sight of the eBolus for best results Any problems please get in touch on 44 0 1392422441 or email michelle ecow co uk eCow Ltd Unit 5b King Street Business Centre 7 9 King Street Exeter UK Version 2 2 3 January 2013 LIMITED Electronic Cow Management eCow Appendix A Configuration Configure the eBoluses to have a smaller time window e g 08 00 12 00 as this has been found to improve battery life This responsive time window just means when the radio for wireless communication is switched on The eBolus still logs 24 7 at a 15 minute interval or more frequently if you would prefer Our other customers normally interact with
17. selecting the white text box in the calibration screen to the left of the Low Point and High Point buttons Place the eBolus into pH 4 buffer and raise to a temperature above 35 C this will activate the eBolus although a few minutes may elapse before the sensing system wakes and decides that it is in this state Press Full Calibration taking 25 minutes per eBolus and follow the instructions or conduct a manual calibration as follows this is quicker fad Calibrate pH Program Calibration Type Next ID Reference pH ADC Average Bolus ID 124 4 Low Point _ 1967 7 High Point 385 Abort Command offset 77297 Full Calibration Gain 52800 Status Exit Calibration On the Calibrate Bolus tab select the bolus ID Press the Low Point button after a while a number will appear in the box This is the value read by the ADC it should be between 1700 and 2000 Repeat this process until the number is not varying by more than 5 Take the bolus out and place it in pH 7 Buffer for a few minutes Then repeat the process of collecting a number for the High Point this number should be between 250 and 550 When it stabilises press Calculate to set the Offset and Gain Press Arm to prepare the eBolus When the status says Armed press Download when the status message says Completed your eBolus is ready to use eCow Ltd Unit 5b King Street Business Centre 7 9 King Street Exeter UK Version 2 2 3 January 2013 L
18. their cows early in the morning so a time window to interrogate the eBoluses to retrieve data would most likely be 08 00 12 00 The device can retain up to 2700 lines of data so please ensure to download data weekly and clear its memory on a routine basis after a successful download Please Note Your system has been factory configured for reliability No responsibility can be accepted should you wish to make any changes to the configuration settings or installation of additional software on the table devices and the like for like replacement warranty will be invalid The software has three operating modes Idle Calibration and Logging and the mode the eBolus operates in depends on the temperature ae i The temperature settings for the modes are ogging Temperature 35 Logging temperature lower temperature at which data logging occurs No upper point Calibration Recommended 35 degrees Temperature High Calibration Temperature Low Calibration Temperature High upper limit for calibration mode Recommended 35 degrees 31 31 C Calibration Temperature Low lower limit for calibration mode Recommended 31 degrees If the temperature of the eBolus falls below this it will enter idle mode to save power no radio It is also possible to configure an idle mode between calibration and logging by selecting different temperatures although this isn t recommended The operating parameters for each mode can b
19. to C Bolus Bolus D dat and right click to open with Notepad Add the ID numbers found on the side of the new boluses and close the file Save on exit The pH Bolus software will need to be closed and re opened to enable the new ID numbers to be available Bi Organize iE Views Y BolusiD Notepad E Documents File Edit Format wiew Help E Pictures demool i Music A More gt Folders w V m a Pl wo Desktop b Bioparametrics a P di Public F aog JE Computer ita FT a amp Local Disk C gt dR Bolus O fh 5 Calibrating the Sensor As stated before all eBoluses have a responsive time window of 00 00 to 23 59 GMT but only log when above 35 C When interrogating an eBolus for the first time ensure it has been warmed for at least 20 minutes between 35 C and 50 C Use an independent thermometer to check the temperature of your heat block water bath You will also need standard buffers of pH 4 and pH 7 Our classic pH buffers 4 and 7 state that there is a change in values as temperature increases pH 4 20degees C 4 00 pH 7 20degees C 7 01 pH 4 30degees C 4 02 DH 7 30degees C 6 98 pH 4 40degees C 4 04 pH 7 40degees C 6 97 eCow Ltd Unit 5b King Street Business Centre 7 9 King Street Exeter UK Version 2 2 3 January 2013 LIMITED Electronic Cow Management eCow But please check your own solution s manufacturers guidelines You can edit these values by
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