User guide - Classic source v2-1
Contents
1. well which makes short work of any semiconductors in the vicinity such as those in your computer for example Our approach is to generate the high voltage pulse INSIDE the lamphouse which is electrically screened and to do so in a way that is balanced with respect to ground This is another tried and tested feature so we can confidently say that it will NOT damage your other equipment It is important to mention here that the two types of lamp that the classic lamphouse supports 75W xenon or mercury xenon continuous and xenon flash require different mountings This prevents the wrong lamp being used with your system However it is quite easy to change the mountings if required It is also possible to upgrade from the Classic to O ptosource as a number of components are identical Please contact our sales department for further details if you are considering this option Page 1 _Page Upgrading 2 Installation guide Before beginning to install a Cairn system it is advisable to have to hand an oscilloscope any age or condition is fine provided that it s not completely dead screwdrivers Allen keys and safety glasses We would also urge our customers to take some time to carefully check the delivery note to make sure that they can identify all the components Having checked that all components are present and correct the next step is to assemble and test the different sections of the system 2 1 Illumination syste2. The Cairn Research Classic Source Lamphouse User Guide 2 1 75 Watt Classic Light Source Aug 2001 Important information Important Please read before installation For maximum reliability we recommend using the equipment within certain guidelines If in any doubt then please feel free to contact our technical support department e mail tech cairnweb com The following points should be considered when using the Classic source 1 Xenon arc lamps are pressurised and a potential hazard Please refer to the relevant section of the manual when changing lamps and always wear suitable protective goggles 2 Hold the lamp by the electrode base to avoid contact with the glass Skin contact will leave behind aresidue which with heat will blacken and therefore reduce light intensity and the lamp s life 3 To avoid arcing between the anode connector arm and the lamphouse position the arm at 45 Pointing to the bottom right hand comer of the assembly when viewed through the quartz window 4 Be careful not to strain the lamp during installation as it is easy to break off the cathode connection if significant bending or twisting forces are applied If you have any problems in installing the lamp please phone for further advice Neither the manufacturer s nor our own guarantee cover lamp breakage during installation so please proceed with CARE 5 Use suitable protective eyewear when focussing the light The short wavelength
3. cccscsssssssssssessssessescsssseescsseseescseseesseseserssseasercaseas i TI Package Comi e iii MI Table Contents aaa v AERC VG CIs sess A ren 1 2 Tnstalaton ro 3 2 1 Illumination system COMPO MCI nn 3 2 2 Illumination system couplings and MOS dada 4 2 3 Lamp Installa ee nee 5 2 4 AA Dea O o OOO 6 2 5 Illumination Set up Sequence caras ii 7 gt Technical SUPPOTt rra e aos 9 m Page v en a Page vi Introduction 1 Introduction The Cairn classic light source comprises of two components namely a lamphouse and an appropriate power supply for the lamphouse In addition components for connecting the light source output to a fluorescence microscope via a epifluorescence port will usually be supplied This connection will take one of three forms A direct coupling an indirect coupling or a beam collimator The direct coupling relays the light from the light guide directly into the rear of the objective the indirect coupling relays the light via aperture and field stops to the objective and the beam collimator relays the light into the entry port of the microscope manufacturers epifluorescence coupling The classic lamphouse has been on sale for the best part of a decade now and it s been very successful We originally designed a 75W lamphouse and power supply because our customers were fed up with having their measurement equipment fried by other manufacturers light sources more about this below This is a thoroughly trie
4. d and tested design which is suitable for many applications and uses our well established 75W power supply with constant power regulation for stable output and long lamp life It uses switch mode technology for compact size minimum heat generation and universal power input requirements 90 250V 50 60Hz Following the introduction of our Optoscan monochromator we began selling more systems to customers with interests in high speed imaging where the highest possible light intensities are required The Classic source actually works well with the Optoscan but the Optosource has been custom designed for this application and gives typically 2 3 times more light the exact improvement depends on the input slit width which is variable in our design For other applications including flash photolysis the Optosource allows more efficient coupling of the light output into a smaller diameter light guide which is likely to be translated into a higher light intensity at the experimental sample From theoretical considerations we expect an approximately two fold improvement For many users the most important feature of our light sources is that they can be used safely in close proximity to any other equipment While xenon lamps typically run at only about 10 20V they require a high voltage trigger pulse of around 30K V to initiate the arc Some other manufacturers seem to ensure that this trigger voltage reaches the lamp by spraying it around everywhere else as
5. fixed to the protruding end of the lamp taking care not to touch the silica envelope surrounding the electrodes To avoid arcing between the anode connector arm and the lamphouse position the arm at 45 pointing towards the bottom right hand comer of the lamphouse when viewed from the mirror face in the same direction as the nodule on the lamp Carefully replace the lamp assembly in the lamphouse and replace the four retaining screws If you inadvertently touch the envelope it is important to clean the lamp with the wipe provided to prevent grease residues burning on to the lamp and thus reducing its operating efficiency Lamp assembly viewed from above 1 Front lamp fitting and arm 2 Rear lamp mount N B It is not possible to connect the lamp in the incorrect orientation or fit the incorrect lamp as the anode and cathode fittings are different for each 2 4 Lamp Removal Ensure the lamp is fully cooled amp protective eyewear is wom before handling To change the lamp after it has reached the end of its useful life remove the lamp assembly as described above and release the anode arm from the lamp Slacken the fixing screw in the cathode mount and the lamp can then be removed through the central hole in the mirror Fitting of the new lamp is as described above in section 2 3 The lamphouse enclosure reaches extremely high temperatures when in use and could cause severe burns so please exercise extreme caution when exchan
6. g it up to the stop Secure in place using the small hex screw Page 7 6 9 If supplied fit the epifluorescence coupling assembly to the microscope see separate instructions provided with the coupling With the light guide mount detached from the microscope epifluorescence coupling feed the free end of the light guide into the light guide mount and tighten in place at the end stop as shown in the figure below Replace the mount with the light guide fitted onto the end of the microscope coupling tube in the epifluorescence port of the microscope To focus the light beam remove one of the microscope objectives and position the appropriate filter cube in the light path Using a piece of fluorescent paper or card at the microscope stage as a target adjust the position of the light guide mount until a sharp image of the end of the light guide is seen at the stage Centre the image using the offset adjustments on the light guide mount Replace the microscope objective and check for uniform illumination at the image plane The illumination path is now fully aligned and the system ready for use To ensure optimum performance from your lamp housing it is recommended that after approximately 1 hour from tum on fine focussing is carried using the detector attached to your system Small adjustments of the horizontal vertical and focussing controls on the lamp should be made to maximise the signal from the detector We recommend tha
7. ging lamps Page 6 Upgrading 2 5 Illumination Set up sequence With the lamp installed in the lamphouse the next stage is to focus the light source 1 3 4 Connect the cabling to the lamphouse There is a four pin lamphouse power lead which needs to be connected from the lamphouse to the power supply unit and the power supply should be connected to the mains If not already in place attach the output coupling tube to the light source with the focussing assembly in place and the diaphragm fully open N B If the focussing assembly is unavailable the lamp should be focussed 55mm in front of the coupling tube using a piece of black card as a target This should be carried out only using suitable protective eyewear to ensure the adjustment can be made again at a future date Tum on the lamp and leave the unit to warm up for at least ten minutes and then adjust the lamp focus and position to give the sharpest image of the lamp arc at the centre of the focussing assembly illustrated below This ensures the maximum amount of light is focussed onto the entrance of the light guide x Unfocussed uncentred x Unfocussed centred Y Focussed centred Turn off the lamp and remove the focussing assembly from the light source coupling before attaching the lamphouse light guide mount in its place together with any filter sliders or filter wheel Fit one end of the light guide into the lamphouse mount slidin
8. m components Lamphouse power supply Front Panel Rear Panel EX CAIRN 1 Power Switch 3 Timer reset 1 Power connector to lamphouse 2 Lamp on indicator 4 Hours of use display 2 Mains power socket Lamphouse Front Panel Rear Panel 1 Output window 1 amp 3 Arc horizontal and vertical adjustments 2 Arc focussing adjustment 4 Arc lamp power supply connector Page 3 2 2 Illumination system couplings and mounts Lamphouse coupling tube Lamphouse light guide coupling with integral iris with provision for two filter sliders Filter slider for lamphouse coupling Liquid light guide Microscope light guide mount Microscope coupling Nikon coupling shown Page 4 xo ya Upgrading 2 3 Lamp Installation As delivery companies are inclined to treat packaged equipment with less respect than perhaps they should we have found it prudent to remove lamps from the lamphouses after testing This saves the inconvenience of finding a sharps bin to empty the lamphouse contents into and ensures you receive both a lamp and lamphouse that are functional on arrival After removing the equipment from its packaging first check the components for any obvious signs of damage If all is well the first stage in the installation is to fit the supplied lamp into the lamphouse Be careful not to strain the lamp during installation as it is easy to break off the cathode connection if significant bending or twis
9. s generated by the arc lamp are potentially hazardous so care should be taken to avoid direct exposure to the beam 6 Do not operate the power supply without a lamp installed in the lamphouse this can lead to equipment damage and failure although you still have to try hard 7 The lamps we supply for use with our light source have a built in filter to cut off the short wavelengths which generate ozone so there is little or no ozone emitted from the light source Page i _Page ii Introduction Il Package Contents Light Source includes lamphouse and power supply a a a Opto 75W Classic 75W Opto 150W Coupling Hardware a a a Cabling a a Diaphragm coupling with focussing assembly Liquid light guide Basic lamphouse light guide coupling with provision for two filter sliders Lamphouse light guide mount with manual shutter amp provision for two filter sliders Lamphouse light guide mount with automatic shutter amp provision for two filter sliders Filter slider for lamphouse coupling Direct epifluorescence coupling for uuenee Microscope Indirect epifluorescence coupling for o Microscope Beam collimator for e Microscope Mains leads Light source power lead Page iii O ao Page iv Introduction Ill Table of Contents I Important Please read before installation c
10. t this fine calibration be repeated periodically during the lifetime of the lamp Page 8 Technical Support 3 Technical Support e mail tech cairnweb com Web http www cairnweb com Address Cairn Research Ltd Unit 3G Brents Shipyard Industrial Estate Faversham Kent ME13 7DZ Telephone 44 0 1795 590 140 Fax 44 0 1795 590 150 Page 9 ___Page 10
11. ting forces are applied If you have any problems in installing the lamp please phone for further advice Neither the manufacturer s nor our own guarantee cover lamp breakage during installation so please proceed with CARE Disconnect the power supply before continuing Arc lamps are pressurised and fragile Ensure suitable protective eyewear is wom at all times while handling 1 To gain access to the lamp mount the rear plate must be removed There are four slotted screws two on each side of the lamphouse which secure this to the housing After removing these screws the rear plate which supports the lamp assembly will slide out of the lamphouse to the rear Location of rear plate retaining screws 2 The lamp is mounted horizontally and is fitted in place by passing the cathode narrower end through the central hole in the mirror and inserting in the mount The lamp is secured by means of a hexagonal screw To reduce shadowing effects ensure the nodule on the glass envelope of the lamp is pointing along the anode arm towards the lower right hand comer of the lamphouse when viewed from the mirror face Hold the lamp by the electrode base to avoid contact with the glass Skin contact will leave behind a residue which with heat will blacken and therefore reduce light intensity and the lamp s life n Page 5 3 The front lamp fitting the anode will be clipped to one side for shipping This should be released xe and
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