MultiMag User Manual
Contents
1. MultiMag Magnetic bead extraction system User Manual Version 1 1 IT IS IMPORTANT TO READ THIS MANUAL BEFORE USING THE MULTIMAG Please read this manual carefully before using the MultiMag and make it accessible to all users Failure to comply with the instructions in this manual will void the manufacturer s warranty and may pose a risk to the user Ensure all users are conversant with the instrument contact Bee Robotics for any fur ther instrument or training requirements if in any doubt before using the instrument Consult User Manual where symbol left is seen on the instrument Bee Robotics Ltd MultiMag User Manual Version 1 1 CONTENTS t INTRODUC ION Nc 3 2 TECGHNIGAL SPECIFICATIONS si cc co5 saad nals o N n dl hedeld ee dence 3 3 UNPACKING AND INSTALLATION i Uto des ead etes gen bea no Pria Dore scuta box Soin eas 4 3 1 UNAS GIS here RE pr UN NE 4 3 2 Environmental Conditoris etia boo eere siste Pei aex e ESEE ea A E 4 3 2 1 BENCINI CO PME M eee 4 3 2 2 WTI ATO meer DENN 5 9 2 9 Eli MCCC 5 3 9 Mial SEUD e E 5 4 SYSTEM OVERVIEW rte eo en See ee eee 6 4 1 mien oreraa M 6 4 2 Back view amp Connections ocean d cxecn eoa iot a pun kasd eu ec Lac vas p apte cu Pone duced 6 4 3 ControlP anel angi KOD EE E s 7 5 ACCESSORIES AND CONSUMABLES 5 5 5 deroan oaae e aaa 7 6 SAFETY amp GENERAL INEORNIATIQDDU uacua ectetuer un2. User Manual Version 1 1 7 GLOSSARYOFMM8TERMS 0 sees Control knob A single control knob for finding See 4 3 and selecting menus and starting stopping op erations Display Displays commands and information Reagent dispensing arm Travels over the extraction and locates two dispensing heads See 4 1 linked to the peristaltic pumps Magnetic pillars Permanent magnets that can See 4 1 be inserted in magnetic pillar tubes Power supply socket Only use the 24V power See 4 2 supply provided Peristaltic pumps Four pumps are located on See 4 2 the back of the MM 8 Extraction tray A disposable plastic tray for extracting and purifying nucleic acids MultiMag Magnetic Bead Extraction System Page 10 Bee Robotics Jan 2010 Bee Robotics Ltd MultiMag User Manual Magnetic pillar tubes A strip of 8 DNAse RNAse free tubes PCR tubes Magnetic pillar tube support Elution strips A strip of 8 of U shaped cups for elution of DNA Elution strip holder Heater block A block in which elution cups or tubes are positioned Normally set to 70 deg C Heater plate A plate positioned underneath the left handside of the extraction that is used to warm lysis buffer in some protocols Reagent Bottle holders MultiMag Magnetic Bead Extraction System Bee Robotics Jan 2010 Version 1 1 Page 11 Bee Robotics Ltd MultiMag User Manual Version 1 1 8 CORRECT FITTING OF PLASTIC CONSUMABLES Please take to f
3. X XX 20 19 TEGHNKSAL SUP POR Prices ERS eS ode oasi hme serae c an ea atado o uote 20 14 APPENDIX qe r 22 14 1 APPENDIX I Synopsis of Symbols used in manual amp on instrument 22 14 2 APPENDIX VII MSDS for typical C100 200 Kit used with the MultiMag 23 MultiMag Magnetic Bead Extraction System Page 2 Bee Robotics Jan 2010 Bee Robotics Ltd 1 INTRODUCTION MultiMag User Manual Version 1 1 The MultiMag is a bench top automated system for extraction and purification of nucleic acids using magnetic bead technology The MultiMag processes one eight sample extraction tray Reagents and wash solutions are added with mixing magnetic bead capture temperature and extraction of unwanted reagents all under automatic software control The result is effective and efficient extraction of the target cell protein or nucleic acid with in the disposable reagent tray The system design provides an efficient and truly walk away instrument with minimal set up and shutdown steps 2 TECHNICAL SPECIFICATIONS Instrument type Processing Capability Processing Time Temperature C Control Processing Volumes Dispensing Mode amp Accuracy Software Voltage amp Frequency Fuses pre m eee WERL ECHTE Stand alone benchtop 1 to 8 samples per run TTT Typically 1 15 minute
4. When the program is paused PROGRAM PAUSED Prompt to restart the program Restart Program p diia Please note during pause some instrument functions will not pause and will continue e g pump priming de priming and heater Press Control Knob PDrosrsm Indicates the program is restarting gra Remark a carousel loading scan is also performed at restarting this stage When the program has restarted PROGRAM RUNNING l Indicates the program is running 9 5 Completion of Extraction Process When the extraction is completed the display shows PROGRAM COMPLETE T Indicates the extraction is completed Press button Pressing the button Control Knob will bring the screen back to the main menu The Heater Block of the MultiMag can reach temperatures of 90 C during a run and is still hot at the end of a run Take care not to touch the heater block Remove elution block Take care as it the temperature may still be 60 deg C Leave on the work bench to cool At this point refer to 9 3 Washing Cleaning MultiMag Magnetic Bead Extraction System Page 17 Bee Robotics Jan 2010 Bee Robotics Ltd MultiMag User Manual Version 1 1 9 5 1 Quick reminder e Connect tubes to 70 isoprop tubes e Leave extraction tray in position e Press Control Knob to start tube washing Finally Remove extraction tray and dispose Remove Magnetic pillar tubes Switch Off power 10 TROUBLESHOOTING 10 1 Error Mes
5. amp consumables BX asune 6 1 2 Detailed Personal Safety Information The Heater plate and block of the MultiMag can reach temperatures of 90 C dur ing a run Take care not to touch the heater plate at any time during or immedi ately after a run Avoid touching the MultiMag with wet hands and DO NOT attempt to disassem ble the MultiMag In either case electrical shock may result and in both cases the warranty will be invalidated It is recommended to wear eye protection a laboratory coat and protective gloves when operating the instrument handling reagents If contact of any re agent occurs with skin or eyes wash thoroughly with water Specimens should be handled as if infectious using safe laboratory procedures Handle all samples as if capable of transmitting disease ALWAYS FOLLOW ACCEPTED HEALTH AND SAFETY PROCEDURES gt gt Pe 6 2 Cleaning amp Disposal The MultiMag may be used with potentially infectious biological samples Ob serve standard laboratory safety practices at all times Cleaning of the MultiMag should be performed regularly using proprietary decontaminants followed by water and appropriate detergent Normally cleaning of the carousel and fixed tray below is most im portant e ALWAYS wear protective gloves prior to cleaning e Use an appropriate detergent to clean surrounding surfaces MultiMag Magnetic Bead Extraction System Page 8 Bee Robotics
6. nua Exeter nue soo eonun 8 6 1 oaiety PrecaldtioliSa doin anon eau auditus date ne boten uu fud cc itd Li o nU ede ce 8 6 1 1 SUIS TADS NA naren ET EE 8 6 1 2 Detailed Personal Safety Information cccccccceeseseeeseeseeeeeeeeeaeaaeeeseeeeeeeeessesaaeaeeeeeeeees 8 6 2 Cleaning DISDOSal zu5xoc Aula di tse it ered ile econ hah De iub seus esata fice ius 8 y GLOSSARY OF MIMS TERMS 5 eet S ideni ettet ao iet tait its os eMe ed 10 8 CORRECT FITTING OF PLASTIC CONSUMABLES eeeeeeeeeeeeenmeeememenns 12 8 1 Magnetic DITAR TUDES e rr p 12 8 2 EUO UID cm eM RN 13 8 3 gt di geled Mi EE 14 92 INSTRUMENT OPERATION siete tee teeta undis Teo Pu uen chis rue a edt eee eae 15 9 1 ey cce E CR 15 9 2 Switching on and selecting the Correct Protocol eeessseseeeeeeeere 15 9 3 A ssa igo Cleaning e cR 16 9 3 1 Ober GIG MIN GS ese toate daca crenata a utens EE mc nixus e ieu selieue inn aeria naa 16 9 4 Pausimng Progra ED EU EE 16 9 5 Gompletion ol Extraction Process ace oe Eee Dc sd meses ets ec ut uc ee ues unies utens 17 9 5 1 Quick reminder S S T S 18 10 TROUBLESHOOTING s cust cne cutie r S a E ette oct antt 18 10 1 Error Messages OVEIVIOW seserian aaa a edu a I A AAE Ea 18 10 2 LASCrUNUMOrMaAON SCEE enia a a areas eeatosesueeatestterossemiemea 18 TE QUICK GUIDE PROTOCOL 5 adiu chica aa eel Alera o added es 19 11 1 Example C100 DNA RNA Virus Kit 19 QJ WEEU RASA S
7. 578 6 R10 Ethanol 64 17 5 200 578 6 R10 Guanidin Hydrochlorid E 50 01 1 x 200 002 3 s R22 36 38 E Wash Buffer White Sodium Perchlorate 76 7601 89 0 o 23 231 511 9 9 Xn Triton X 100 9002 93 1 R22 41 Elution Buffer No hazardous ingredients T069 53 1 Magnetic Beads No hazardous ingredients Wash Buffer Yellow No hazardous ingredients Perchlorate solutions with concentrations below 70 are classified as non oxidizing materials Ill Health Hazard Data Route s of Entry Inhalation Ingestion Skin contact or eye contact from splashes Inhalation Ethanol may cause cough drowsiness headache or fatigue Guanidine Hydrochloride Triton X 100 and Sodium perchlorate may cause respiratory tract irritation Ingestion Guanidine Hydrochloride Triton X 100 and Sodium perchlorate may cause gastrointestinal irritation with nausea vomiting and diarrhea Skin contact Ethanol may dry skin cause skin discoloration with irritation Guanidine Hydrochloride Triton X 100 and Sodium perchlorate may cause reddening discomfort or mild irritation Eye contact MultiMag Magnetic Bead Extraction System Page 23 Bee Robotics Jan 2010 Bee Robotics Ltd MultiMag User Manual Version 1 1 Splashes may cause reddening discomfort or eye damage Carcinogenicity NTP IARC Monographs OSHA Regulated not listed N A N A N A Chronic Exposure Ethanol may cause drying cracking of skin Repeated high exposur
8. Jan 2010 Bee Robotics Ltd MultiMag User Manual Version 1 1 e Use a cloth dampened in detergent and wipe all areas in and around the extraction tray area Use the same technique to wash all parts that may come in contact with any accidental spillage The instrument will automatically clean all of the reagent and waste lines provided the user follows the onscreen instructions see 9 8 at the end of each run All the hazardous substances will be contained in the waste bags and in the reagent containers should any reagents be left at the end of any run When disconnecting waste bags at the end of any run disconnect the waste bag connector luer then replace with luer caps provided in every kit on inlet of waste bags and handle waste bags very care fully do not squeeze under any circumstances Replace waste bags per kit used No other drainage system is required with waste amp reagent disposal from the instrument The maxi mum waste in any waste bag is 250ml and a typical reagent kit will produce approx 360ml of waste divided into two 250ml bags DISPOSAL Dispose of any unused reagents and waste in accordance with country federal state and local regulations Instrument Disposal W As part of the WEEE EU directives this instrument should be returned to the manufacturer for dismantling at the end of its life C MultiMag Magnetic Bead Extraction System Page 9 Bee Robotics Jan 2010 Bee Robotics Ltd MultiMag
9. Waste Module Black On Control Panel near Power Green LED Power Indica tor T On Control Panel near Symbol c Muling Pause Yellow LED Program Paused Indicator Symbol amp Outline On Control Panel near entry slot of Smart Card Symbol amp Outline White Insert Smart Card On Control Panel around the Control Knob Symbol amp Outline White Rotate amp Press MultiMag Magnetic Bead Extraction System Page 22 Bee Robotics Jan 2010 Bee Robotics Ltd MultiMag User Manual Version 1 1 14 2 APPENDIX VII MSDS for typical C100 200 Kit used with the MultiMag Material Safety Data Sheet I Product Identification Product Name As Used on Label and List Catalog Number Virus DNA RNA Extraction Kit C100 200 Manufacturer s Name Bee Robotics Ltd Address Telephone Number for Information 32 33 Cibyn Industrial Estate Caernarfon 44 0 1286 672744 Gwynedd Wales UK LL55 2BD Emergency Telephone Number 44 0 1286 672744 ll Hazard Ingredients Identity Information Labelling according to EC Directives Guanidin i 50 01 1 200 002 3 R22 36 38 10 17 Xn R22 41 Reagent Hazardous Components CAS No EC No Content Lysis Buffer e 7 Triton X 100 9002 93 1 1 296 Sorbitan Sesquioleate 8007 43 0 R36 37 38 2 Binding Buffer Sodium Perctiorae 7601880 231 5119 M Green Sodium Perchlorate 7601 89 0 231 511 9 R25 Ethanol 64 17 5 200
10. bent material e g vermiculite dry sand to contain pick up the spilled solution Place all contaminated disposables into a suitable container seal label and hold for dis posal Waste Disposal Method Please consult local state and federal regulations for additional guidance on disposal VIII Precautions to be taken in Handling and Storing Store in tightly closed containers Ethanol take measures against electrostatic loading See package insert for further storage and handling information IX Control Measures Respiratory Protection Respiratory protection is not required under normal use of this product Ventilation A system of local and or general exhaust is recommended Local exhaust ventilation is generally preferred because it can con trol the emissions of the contaminant at its source Protective Gloves Eye Protection Wear appropriate eye pro Wear appropriate protective gloves to tection to prevent eye contact prevent skin contact Other Protective Clothing or Equipment Wear appropriate body protection to prevent skin contact Eye wash stations and deluge showers Work Hygienic Practices Good laboratory technique should be used when handling this product Observe appropriate chemical hygiene Avoid contact with skin or eyes Do not place in mouth Do not eat drink or smoke while working with product Upon completion of work activities involving this product wash hands thoroughly with soap and water X Physical C
11. d 32 33 Cibyn Caernarfon Gwynedd Wales UK LL55 2BD Tel 44 0 1286 672744 Fax 44 0 1286 678322 Web www beerobotics com E mail enquiries beerobotics com There are no user serviceable parts within the MultiMag MultiMag Magnetic Bead Extraction System Page 20 Bee Robotics Jan 2010 Bee Robotics Ltd MultiMag User Manual Version 1 1 Only by Bee Robotics or associated trained service personnel may repairs and servicing be carried out For further details of instrument or to arrange for training to be given on the instrument con tact Bee Robotics at above address MultiMag Magnetic Bead Extraction System Page 21 Bee Robotics Jan 2010 Bee Robotics Ltd MultiMag User Manual Version 1 1 14 APPENDIX 14 1 APPENDIX I Synopsis of Symbols used in manual amp on instrument Symbol Publication Symbol amp Outline IEC 60417 5032 Alternating Current Instrument Serial Label Symbol amp Outline IEG 60417 5007 On Supply Inlet Filter Symbol amp Outline IEC 60417 5008 Off Supply Inlet Filter Background Yellow Symbol amp Outline IEC 60417 5032 Caution Hot Surface On Heater Plate Black Background Yellow On Waste Station Symbol amp Outline IEC 60417 5007 Caution Risk of Danger On Control Panel near y Red LED Symbol amp Out Black siti line White no bgcolour Background Yellow Symbol amp Outline ISO 7000 0659 Biohazard On
12. d is ready If a malfunction is detected an error message will be displayed which may require the attention of a qualified service engineer However refer to section 13 TECHNICAL SUPPORT for more information and help MultiMag Magnetic Bead Extraction System Page 5 Bee Robotics Jan 2010 Bee Robotics Ltd MultiMag User Manual Version 1 1 4 SYSTEM OVERVIEW 4 1 Front View 4 2 Back View amp Connections One of four peristaltic pumps Power switch 24Volts Computer USB connection Power cable connector Pumps 1 4 are used for the transfer of liquid reagents to the extraction tray All pumps can be purged manually using the control panel MultiMag Magnetic Bead Extraction System Page 6 Bee Robotics Jan 2010 Bee Robotics Ltd MultiMag User Manual Version 1 1 4 3 Control Panel and Knob Each extraction protocol can be located and selected us ing the control knob and display There is an LCD display showing instrument status prompts and input requirements There is a single control knob which functions by turning or pressing Single Control Knob 5 ACCESSORIES AND CONSUMABLES MultiMag Magnetic Bead Extraction System Page 7 Bee Robotics Jan 2010 Bee Robotics Ltd MultiMag User Manual Version 1 1 6 SAFETY amp GENERAL INFORMATION 6 1 Safety Precautions 6 1 1 Instrument labelling Please observe and respect all caution labels shown on instrument
13. e may damage liver nervous system or blood cells Sodium perchlorate Prolonged or repeated exposure may cause thyroid inhibition IV Medical Conditions Generally Aggravated by Exposure Persons with pre existing skin disorders eye problems or liver function may be more susceptible to the effects V Emergency and First Aid Procedures Inhalation If breathing becomes difficult remove victim to fresh air Seek medical assistance immediately Ingestion If victim is conscious and alert give 2 4 cupfuls of milk or water Never give anything by mouth to an unconscious person Get medical aid immediately Skin contact If skin contact occurs wash skin with water and remove contaminated clothing If contact with product leads to continued reddening inflammation after rinsing with water get medical attention Eye contact Flush eye s with large volumes of water for at least 15 minutes Get medical attention immediately VI Fire fighting procedures Extinguishing Media Use dry chemical carbon dioxide CO or appropriate foam Special Fire Fighting Procedures Wear self contained breathing apparatus and full protective gear Unusual Fire and Explosion Hazards Auto ignition temperature of ethanol 423 C VII Precautions for Safe Handling and Use Steps to Be Taken in Case Material is Released or Spilled Wear appropriate personal protective clothing as specified in section IX Use non sparking tools and equipment ethanol Use an inert absor
14. ect programme number The instrument will now complete the purification process Eluted samples The eluted samples in the eluting strip or tubes are now ready for RT PCR or PCR Storage If the samples need to be stored then the tube option should be used available in 2010 Each tube will fit into a labelled screw cap tube available from BeeRobotics Closing down or continue with next run MultiMag Magnetic Bead Extraction System Page 19 Bee Robotics Jan 2010 Bee Robotics Ltd MultiMag User Manual Version 1 1 If more samples are to be extracted check carefully that there is enough reagent in each bottle and mix the bead binding before starting new run Dispose of extraction tray and magnetic pillar tubes ready for a new set If no more extractions are required then leave extraction tray in position Disconnect green and white connectors and re connect to yellow 15ml tubes Its not necessary to reconnect the red and yellow bottles Select Wash The tubes will be washed out with yellow buffer Dispose of extraction tray Power down MultiMag 12 LIMITED WARRANTY Please note the serial number of the MultiMag found on the back of the instrument below for future reference SEIBDAEJTIO aiebant iE roe E xA aD d dd tania Warranty Bee Robotics Ltd warrants that each product described herein will be free from defects in materials and workmanship for a period of one year from the date of delivery Bee R
15. he MultiMag in the pre PCR Laboratory on a solid flat surface Place the instrument so that there is no obstruction to the Control Panel on the top of the instrument The minimum bench space required is 600mm width and 400mm depth Ideally the bench space should be 800mm width x 450mm depth Place the instrument a maximum of 1 metre from an appropriate power source The cover lid swings upwards so that when at its maximum opening the height from the instrument base to the extreme of the lid is 350mm 3 2 1 1 Disconnection guidelines In an emergency immediately turn the power off and unplug from power source The mains switch and mains lead connection is on the bottom left of the instrument to the left hand side as viewed from the front The instrument should be 120mm from any rear wall so that there is ample clearance for the mains and waste module connectors and to facilitate disconnection in an emergency Do not place the instrument too close to any object that might impair emergency discon nection in an emergency particularly any object to the right or rear of the instrument MultiMag Magnetic Bead Extraction System Page 4 Bee Robotics Jan 2010 Bee Robotics Ltd MultiMag User Manual Version 1 1 3 2 2 Ventilation Do not place the instrument in line with a direct draft such as an air vent Otherwise there are no spe cific ventilation requirements for the instrument Allow a space of 100mm between the instrument and any wal
16. he following mes sage is displayed This routine checks basic software and mechanical functions MultiMag Invites user to press Knob to initialise the instrument Press Knob Lower lid and press Knob The instrument performs a system self check MultiMag For about 10 seconds Initialising Notes in the event of a power failure deliberate power cut or error a message is displayed before the system check as a reminder Try overriding this message by pressing the Control knob and observe whether the instrument initialises properly Refer to the section Last run information screen chapter 10 2 p18 __ MultiMag Program No XX Invites user to select a protocol Turn Control Knob Clockwise to select correct protocol MultiMag __ Example of a protocol for viruses C100ul Virus v2 uiuis Press Control Knob PROGRAM SETUP Select number of loaded samples by turning the knob No of samples xx Press Control Knob MultiMag Magnetic Bead Extraction System Page 15 Bee Robotics Jan 2010 Bee Robotics Ltd MultiMag User Manual Version 1 1 Program NO XX Asks user to confirm right number of samples and cor No of samples YY rect programme number Press Control Knob to activate protocol Note This should not be attempted unless the following sections have been read and understood 9 3 Washing Cleaning Locate 15ml tubes containing 7096 isopropanol water in the appropriate wash p
17. hemical Characteristics Boiling Point N A Vapor Pressure mm Hg N A Vapor Density AIR z 1 N A Specific Gravity HO 1 N A Melting Point N A Evaporation Rate Butyl Acetate 1 N A Solubility in Water Soluble in water Appearance and Odour Magnetic Beads suspension of brown polymer particles in water MultiMag Magnetic Bead Extraction System Page 24 Bee Robotics Jan 2010 Bee Robotics Ltd MultiMag User Manual Version 1 1 All other reagents all bottles contain aqueous solutions XI Reactivity Data Stability Stable under normal handling and storage conditions Incompatibility Materials to Avoid Ethanol strong oxidizing agents potassium dioxide bromine pentafluoride acetyl bromide acetyl chloride platinum sodium perchlorate strong acids finely powdered metals magnesium reducing agents Perchlorate solutions with concentrations below 70 are classified as non oxidizing materials Guanidine hydrochloride sorbitan sesquioleate strong oxidizing agents Hazardous Decomposition or Byproducts Guanidine hydrochloride Hydrogen chloride nitrogen oxides carbon monoxide irritating and toxic fumes and gases carbon dioxide Sodium perchlorate Hydrogen chloride chlorine oxides of chlorine sorbitan sesquioleate carbon monoxide carbon dioxide Hazardous Polymerization Conditions to Avoid no Heat flames ignition sources incompatibles XII Toxological Information Toxicity Data Sodium perc
18. hlorate undiluted LD50 rat oral 2100 mg kg Guanidine Hydrochloride undiluted LD50 mouse oral 571 mg kg LD50 rat oral 475 mg kg Ethanol TDLo man 1 4 g kg LD50 rat oral 7060 mg kg Irritation rabbit skin 20 mg 24H moderate reaction rabbit eye 500 mg 24H mild 500mg severe Reproductive Effects Ethanol has been linked to birth defects in humans Carcinogenicity No XIII Ecological Information Environmental fate stability Ethanol is expected to readily biodegrade when released into soil and water and is degraded when released into air Effect on material on plants or animals N A Effect of chemical on aquatic life Ethanol is not expected to be toxic to aquatic life The LC50 96 hour values for fish are over 100 mg L XIV Disposal considerations Product There are no uniform EC Regulations for the disposal of chemicals or residues Chemical residues generally count as special waste The disposal of the latter is regulated in the EC member countries through corresponding laws and regulations We recommend that you contact either the authorities in charge or approved waste disposal companies which will advise you on how to dispose of special waste Packaging Disposal in compliance with official regulations Handle contaminated packaging in the same way as the substance itself If not officially specified differently non contaminated packaging may be treated like household waste or recycled XV Tran
19. it these components correctly ALWAYS USE GLOVES 8 1 Magnetic pillar tubes Note the magnetic pillar tubes are DNase and RNAse free PCR tubes Treat very carefully to avoid contamination Carefully slide Magnetic pillar tubes into white plastic support Check that the two components have meshed together properly Position magnetic pillar tube holder on the instrument underneath the magnetic pillars Check that the mechanism is working smoothly by lifting up and down If there is evidence of snagging inspect carefully and relocate the white plastic support properly MultiMag Magnetic Bead Extraction System Page 12 Bee Robotics Jan 2010 Bee Robotics Ltd MultiMag User Manual Version 1 1 8 2 Elution strips Note the elution tubes are DNase and RNAse free PCR tubes Treat very carefully to avoid contami nation Note carefully that the strip is notched at the 1 first cup position The 1 cup position should be positioned away from the user Mark the notched end with an indelible pen to identify the first tube Position the elution strip in elution strip holder Locate elution strip holder in the heater block Note The small handle is nearest the user The small handle is used to position remove the elution strip holder MultiMag Magnetic Bead Extraction System Page 13 Bee Robotics Jan 2010 Bee Robotics Ltd MultiMag User Manual Version 1 1 8 3 Extraction Tray Label the tray with sa
20. l or other instruments 3 2 3 General Keep free of dust harsh solvents and acidic vapours Vibration and harsh sunlight must be avoided to ensure correct results The MultiMag should always be placed on a flat stable surface The MultiMag is intended for indoor use only and should not be subjected to unduly large variations in temperature and humidity This instrument was designed for use in a typical laboratory environment i e Altitude Up to 2000m Temperature Ambient temperature range Between 5 30 C T Maximum relative humidity 80 for temperatures up to 31 C decreasing linearly y to 50 relative humidity at 40 C Power Leave to stand for 3 hours before applying power to avoid problems that might arise from condensa tion The MultiMag meets the protection requirements laid down in the European Council directive 89 336 EEC on electromagnetic compatibility and carries a CE mark Storage conditions from 5 C to 30 C 3 3 Initial Setup After unpacking according to 3 1 above place instrument on a bench according to 3 2 above Connect the 24V supply to the mains socket and the back of the instrument Lower the instrument lid Switch On the mains switch at the back of the instrument Press Knob MultiMag Initialising is displayed on the LCD screen This takes a few seconds only At the end of the initialization routine the LCD screen displays MultiMag Press Knob indicating the instrument has been checked an
21. mple identification Carefully place appropriate volume of sample in the correct position starting at position A1 and pro ceeding to position H1 Note It is not very efficient to use fewer than 4 samples however an extra 20 volume of re agent is provided so that this should be sufficient of 4 2 sample runs Waste and purge trough Slide the extraction tray into the extraction tray guide rails as shown Correct position of tray Push as far in as possible without too much force into the guide rails Remove for safe disposable by sliding tray towards you Be careful Do not spill contents MultiMag Magnetic Bead Extraction System Page 14 Bee Robotics Jan 2010 Bee Robotics Ltd MultiMag User Manual Version 1 1 9 INSTRUMENT OPERATION 9 1 Overview The MultiMag ON OFF mains switch is located on the back of the instrument The MultiMag is operated by a single knob on the control panel on the front of the instrument The control knob functions by turning clockwise or anticlockwise or by pressing down Turning The control knob clockwise or anticlockwise will change a screen display e tothe next or previous screen display or e todisplay either Yes or No or e to select a number e g no of samples Pressing the control knob indicates acceptance of the display message 9 2 Switching on and selecting the Correct Protocol After switching on the MultiMag at the mains switch on the back of the instrument t
22. obotics Ltd agrees as its sole responsibility under this limited warranty and upon prompt notice of the defect to repair or re place any product found to be defective within the warranty period The limited warranty is not applicable to 1 abnormal wear and tear 2 abuse unreasonable use improper installation mistreatment or neglect 3 damage caused by equipment or system with which the product is used 4 damage caused by modification or repair not made or authorised by Bee Ro botics Ltd or 5 theft vandalism fire water or other peril Product may not be returned without proper authorisation from Bee Robotics Ltd Cost of transportation removal or reinstallation of the equipment will be paid by the purchaser This warranty and the remedies set forth herein are exclu sive and in lieu of all other express or implied including any implied warranties or merchantability or fitness for a general purpose and no other representations or claims shall be binding on or obligate Bee Robotics Ltd in any way In no event will Bee Robotics Ltd be liable for any special incidental or consequential damages resulting from use or malfunction of this product or the equipment or sys tem with which it is used loss of revenue or cost of replacement of goods 13 TECHNICAL SUPPORT Manufactured By BEE ROBOTICS LIMITED 32 33 Cibyn Caernarfon Wales UK For all technical support and assistance contact Technical Department Bee Robotics Lt
23. ositions Connect tube connector to the 15ml tube cap connector Extraction tray set up Quick Demo Demo Program Position an empty extraction tray in the instrument End of Run wash Cleaning Program Leave the used extraction tray in position Turn Control Knob MultiMag Protocol for washing tubes at the end of an extraction Cleaning Program run Press Control Knob Proceeds to pump 7096 alcohol through the tubes 9 3 1 Other Cleaning In case of contamination of the work surface wipe with a 3 596 solution of hydrogen peroxide Do not leave for extended periods In addition if contamination is a recurring problem place the MultiMag in a cabinet that has an UV source leave the MultiMag lid open and sterilise with UV light during pref erably night time hours 9 4 Pausing Program During a run the program can be paused by pressing the control knob for at least 1 second Push Control Knob for 1 second Fouge ihi a2 Prompt to continue the program Pressing the control knob the system returns to the Program running display Turn Control Knob Pause a 2 Sues Prompt to pause the program By pressing the control knob the system pauses the program MultiMag Magnetic Bead Extraction System Page 16 Bee Robotics Jan 2010 Bee Robotics Ltd MultiMag User Manual Version 1 1 Press Control Knob PROGRAM PAUSING Indicates program pausing is in progress Please wait oe d dii
24. s to 25n minutes M S S S I I LII Programmable fi from ambient to 90 C EE 90utoi5ml bindngstag Peristaltic pumps T 2 i Menu driven embedded software ee Bench Top Power Supply 100 240V 47 63Hz Input 24V 100W Output 170 H x 330 D x 530 W m 10kg MultiMag Magnetic Bead Extraction System Page 3 Bee Robotics Jan 2010 Bee Robotics Ltd MultiMag User Manual Version 1 1 3 UNPACKING AND INSTALLATION 3 1 Unpacking CAUTION Take care when removing from its container 1 Visually inspect the container for transit damage 2 Place the carton in an upright position to open Lift out the instrument and at the same time remove any protective foam and gently rest the in strument down on the bench 4 Inspect the instrument for any obvious signs of transit damage 5 Report any damage immediately to your local representative 6 Check that the serial number on the instrument and the delivery note are the same 7 Check that all accessories are present Contents should be as follows MM8 MultiMag Automated Magnetic Bead Extraction System MM8 BHS Bottle Holder x2 MM8 PSU 24V 100W Bench top Power Supply ML 10 Mains lead for 24V power supply USBL 2M USB Lead 8 Store packing materials until you are completely satisfied that the instrument was delivered safely and that it is performing to specification 3 2 Environmental Conditions 3 2 1 Bench Space Always place t
25. sages overview In the event of a system failure or fault the MultiMag will alert the user as follows e An audible signal will be heard e The LCD screen on the control panel will display an error message described below example 0908000000111111 The user must make a note of the error code and report 2244445509996666 it as soon as possible to the local agent or Bee Robotics e Usually errors mean that the instrument has to be restarted Before performing any other runs consult the local agent or Bee Robotics to determine the cause of the error 10 2 Last run information screen In the event of an error during the previous run a message is displayed as a reminder before the Sys tem Check after switching on the MultiMag INFO LAST RUN Indicates there was a power failure during the last run POWER FAILURE and the program could not be completed INFO LAST RUN Indicates there was an error during the last run GENERATED ERROR It is advisable to contact a service engineer Press Control Knob The instrument performs a system check as usual MultiMag System Check Either MultiMag Magnetic Bead Extraction System Page 18 Bee Robotics Jan 2010 Bee Robotics Ltd MultiMag User Manual Version 1 1 11 QUICK GUIDE PROTOCOL All the kits sold by BeeRobotics have a similar format as follows Bottle Color Code Lysis buffer sometimes combined with bead binding in Green bottle Bead and Binding buffer some
26. sport Information Is this material hazardous Yes Proper shipping name Ethanol Hazard Class Number 3 Packaging group II UN Number UN 1170 XVI Other Information MultiMag Magnetic Bead Extraction System Page 25 Bee Robotics Jan 2010
27. times contains lysis agent A wash buffer containing chaotropic salts and other wash consitituents Alcohol based buffer Elution buffer 11 1 Example C100 DNA RNA Virus kit Reagent preparation Mix the magnetic beads with the binding buffer Green Fitting plastic consumables See section 8 Reagent position on instrument Follow the color guide on the round black bottle holder Note To aid mixing of the bead binding buffer always use a 15 ml tube and use an approximate vol ume for the run 1ml excess Position each bottle in the Reagent bottle holder according to the colour code Connect each tube according to color code Sample preparation Remember to label reagent tray starting with A1 and proceeding to H1 Use 100ul of diluted viral transport medium This assumes 1 nose throat swab per 1 3ml Other samples such 10ul buccal cells diluted with 80ul water may also be used Add 100ul lysis buffer and 5 ul PK to each sample and incubate for 10 minutes at 55 60 deg C in an air incubator Instrument start up Select the appropriate Protocol C100 Virus Wait until the lysis is completed and position the extraction tray in the instrument see section 8 3 Complete the start up process by responding to the last prompt PROGRAM No XX No of samples YY Select number of loaded samples by turning the knob Press Control Knob Program NO XX Asks user to confirm right number of samples and cor No of samples YY r
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