GenTarget's EcoTMPlasmid DNA Miniprep Kit User Manual
Contents
1. HEK293 YFP Stable Cell Line Catalog Number Storage ScC011 1 vial of cells 2 x 10 cells in 80 Liquid nitrogen DMEM 10 FBS 10 DMSO Product Description The HEK293 Cell Line is a stable line established from primary embryonal human kidney transformed with sheared human adenovirus type 5 DNA The expressed E1A adenovirus gene allows these cells to produce very high levels of protein HEK293 YFP cell is transformed from the 293 cell line and stably expresses YFP gene It is established by transduction of YFP expressing lentivirus that also contains a blasticidin resistant gene for selection and maintenance of the stable cell line YFP is constitutively expressed in high level under CMV promoter The following expression construct was integrated into cell s genome Core expression cassette 500nm 535nm Ex Em filter Bright light Culture procedure 1 Thaw the frozen vial of cells quickly in a 37 C water bath 1 3min decontaminate the outside of the vial with 70 ethanol AMSBIO www amsbio com info amsbio com By UK amp Rest of the World BEE North America German Switzerland ES 184 Park Drive Milton Park 1035 Cambridge Street sik g Landstr 17 19 Centro Nord Sud 2E Abingdon OX14 4SE UK Cambridge MA 02141 60325 Frankfurt Main CH 6934 Bioggio Lugano T 44 0 1235 828 200 T 1 617 945 5033 or T 49 0 69 779099 T 41 0 91 604 55 22 F 44 0 1235 820 482 T 1 800 987 0985 F 49 0 69 1332. This product is limited to the laboratory that the product is delivered to This Product is not for resale or distribution transfer for any purpose including transfer of the Product as a component of any products Amsbio will retain all rights for this Product s license and other intellectual property 4 This Product should be used for non profit purpose including any products and services usage Furthermore research use only means that this product is excluded without limitation from resale repackaging or modification to be used for the making or selling of any commercial product s or services without the written approval of Amsbio You may contact us at info amsbio com for product proprietary information 5 Amsbio is not liable and does not have any responsibility or liability whatsoever for any direct and indirect consequential or other damages resulted from using this Product AMSBIO www amsbio com info amsbio com UK amp Rest of the World BE North America m Germany Switzerland AS 184 Park Drive Milton Park 1035 Cambridge Street Bockenheimer Landstr 17 19 Centro Nord Sud 2E Abingdon OX14 4SE UK Cambridge MA 02141 60325 Frankfurt Main CH 6934 Bioggio Lugano T 44 0 1235 828 200 T 1 617 945 5033 or T 49 0 69 779099 T 41 0 91 604 55 22 F 44 0 1235 820 482 T 1 800 987 0985 F 49 0 69 13376880 F 441 0 91 605 17 85 F 1 617 945 8218
3. 76880 F 41 0 91 605 17 85 F 1 617 945 8218 2 Transfer the entire content of the cryovial into a T 75 cm flask containing 15 ml of pre warmed complete medium Incubate the cells overnight in a 37 C incubator 5 CO3 3 The following day replace the medium with 15 ml of pre warmed complete medium Optional add 10 ug ml blasticidin in medium 4 Incubate the cells and monitor cell density Passage cells 1 10 dilution when the culture reaches 80 90 confluency 6 Freeze cells at a density of 3 x 10 cells ml using 90 complete medium with 10 DMSO A Complete medium D MEM high glucose 2mM L glutamine 10 Fetal Bovine Serum FBS 0 1 mM MEM Non Essential Amino Acids NEAA 1 Pen strep optional Quality Control Each vial contains greater than 2 x 10 cells with gt 95 viability before freeze Cells are tested free of bacteria viruses mycoplasma Warranty and user terms 1 This product is warranted to perform as described when used in accordance with this manual Amsbio MAKES NO REPRESENTATIONS AND EXTENDS NO WARRANTIES OF ANY KIND EITHER EXPRESS OR IMPLIED Amsbio s sole remedy for breach of warranty should be at the option of Amsbio to repair or replace the product if this product does not meet the stated quality standard 2 By paying the purchase price buyer is granted a non transferable non exclusive license to use the product This product is sold for research and development purposes only 3
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