FlexChem User Guide
Contents
1. over tighten SciGene Technical Service 408 733 7337 408 733 7336 Fax techserv scigene com www scigene com Page 3 Hybex Microarray Chamber User Guide Step 5 Attach Handle to Chamber amp Insert into Heating Unit Screw the chamber handle into the top of del the assembled chamber Carefully lift the o chamber keeping it level and insert it into Chamber the heating unit by aligning the recess for i Assembly the temperature probe Unscrew the handle Lae and lower the heated lid aA Align recess in chamber with temperature The heating unit must have two e ea Cee i TA center of A chambers inserted for proper a BZA heating unit operation Turn the power switch on the front of the Aer eena heating unit to the ON position Select the N two chambers desired temperature by pressing the up and Ie turn on power down buttons while holding in the SET button The unit will attain the desired temperature and stabilize in a few minutes Hold in the SET key and use the UP DOWN buttons to set the desired temperature Step 7 Remove and Disassemble the Chamber At the conclusion of the desired incubation Use the T handle time screw the handle into the top of the hex wrench to chamber a few turns and carefully lift it from remove corner the unit Unscrew the cover screws and lift PON the cover from the base If the chamber remains adhered to the base use the2. rack on the chamber base so the two screw heads come through the key holes Move rack toward the center of the base to secure and then rotate base 180 and add a second rack if needed Prepare coverslipped arrays using your desired volume of target probe solution and hybridization buffer Keeping the slide level and holding it at its ends slowly and evenly push the slide into one set of side clips all the way into the rack As an aid for pipetting and handling place Slides are held the array across an extra rack as shown so in place by the ends are easy to grab Place array on Hold slide by the spring clips extra rack when ends and insert i pipetting into rack Failure to insert the slide completely into the rack will interfere with the chamber cover Step 3 Add Water to Cover Pad Invert the chamber cover with absorbent pad in place and add 1 ml of dH20O Allow iqui Absorbent Pad liquid to soak completely into the pad AEE Place the chamber cover with water impregnated pad in place onto the base by aligning the recess for the temperature Recess for probe Temperature Use the T handle hex wrench to tighten the four corner screws on the cover Tighten the first screw a half turn then move to the screw diagonally across and tighten a half Tighten screws with turn Continue with the opposite corners T handle Hex Wrench Repeat until wrench stops or hand tight Do not
3. 0 6ml Tube Heat Block Holds 60x0 6 mL tubes 1 5ml Tube Heat Block Holds 32x1 5 mL microcentrifuge tubes Waterbath Insert 625 mL Hybex Microarray Chamber Kit Includes 2 Chambers Racks And Tools Slide Rack Holds 4 Slides Absorbent Pads For Chamber Cover Handle For Hybex Slide Racks T Handle Hex Wrench 1051 52 0 Digital Thermometer Includes Cable and NIST certificate Send orders to Fax 408 733 7336 or custserv scigene com SciGene Technical Service 408 733 7337 408 733 7336 Fax techserv scigene com www scigene com
4. SciGene Version 2 1 June 2009 Hybex Microarray Chamber User Guide Read This Section First Handling Arrays and Racks Look closely at the construction of the rack that holds the arrays Slides are inserted into four pairs of spring clips on the sides of the rack Holding a test slide at its ends with thumb and forefinger insert it into the spring clips Practice this a few times Slides should insert smoothly and be gently held by the clips Maintaining System Humidity Maintaining a good seal between chamber cover and base is essential to performance The chamber base o ring and the edge of the cover that seals against it must be free of debris or damage to provide an effective seal and maintain humidity in the chamber The o ring and chamber cover edge should be inspected and wiped clean with a Kimwipe or other lint free paper towel before each use Take care not to damage the cover sealing edge when not in use Lastly be sure to pipet 1 ml of water into the cover pad before use to prevent samples from drying out Proper Use of the Heating Unit Turn on power and set temperature only after inserting the chambers into the unit Two chambers must be used surrounding the temperature probe in the middle of the heating unit to ensure accurate temperature control Do not preheat the heating unit or chambers before use Replacing Absorbent Pad Cleaning and Storage Chamber cover with absorbent pad removed chamber
5. base with o ring in place and racks should be washed in warm water with mild detergent rinsed with dH20 and dried using a lint free cloth Do not use organic solvents that may damage the o ring or finish When not in use insert racks into the chambers loosely assemble cover and base place in a Ziploc bag and store in a Safe location The absorbent pad in the chamber cover should be replaced when it appears worn Unscrew the nylon screw and discard the worn pad With the cover turned over position a new pad so its mounting hole is above the threaded hole in the cover Secure the pad to the cover with the nylon screw using a Phillips screwdriver Do not over tighten Identify System Components Use the illustration below to identify system components at RP650 0670 05 J 7 Chamber handle a RP100 0670 01 Cover socket cap screw with retaining ring Chamber cover RP1057 32 1 Absorbent pad 7 RP650 0670 2 Cover pad screw 1057 32 3 Slide rack 4 position RP650 034 01 Rack alignment screw RP100 2004 01 yp A Chamber base O ring ERNS F Chamber base L RP650 0670 06 T handle hex wrench RP1057 32 5 _ Handle for Hybex racks SciGene Technical Service 408 733 7337 408 733 7336 Fax techserv scigene com www scigene com Page 2 Hybex Microarray Chamber User Guide Step 1 Insert Rack s Into Chamber Base Place a slide
6. flat edge of a small screwdriver to gently separate the cover from the base SciGene Technical Service 408 733 7337 408 733 7336 Fax techserv scigene com www scigene com Page 4 Hybex Microarray Chamber User Guide Step 8 Removing and Handling the Rack s A Slide the rack s toward the edge of the base and lift to release B Move the slide rack s immediately to a waterbath insert Cat 1057 35 0 inside a second Hybex heating unit or to another preheated container of wash solution Lay each rack on its back to allow the Place rack into wash solution in waterbath coverslips to fall away from the slides and exit BEE LOWER O through the bottom of the rack its back to allow coverslips to fall away C With racks submerged align the two semicircular C tabs on the side of one rack with the corresponding slots on the opposite side of the second rack Insert the spring handle Cat RP1057 32 5 into the a center slots on the long side of the racks The handle Se A will hold two racks together while processing handle Ordering Information O g PF Description UOM Hybex Microarray Incubation System 115V Complete System Hybex Microarray Incubation System 230V Complete System Hybex Microsample Incubator base 115V Heating base only Hybex Microsample Incubator base 230V Heating base only PCR Tube Block Holds 96x0 2 mL tubes or single plate
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