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H-SERIES USER'S GUIDE

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1. SPEED CONTROL VOLTAGE CHART Q3 Q2 Qi Analog 30RPM 45RPM 60 RPM Input SPEED SPEED SPEED V 0 0 0 0 OFF OFF OFF 0 0 1 53 5 8 12 0 1 0 75 7 5 12 17 0 1 1 1 09 10 15 26 1 0 0 1 43 15 23 35 1 0 1 1 72 20 30 42 1 1 0 2 08 23 35 48 1 1 1 2 5 25 45 60 41
2. Cs ES SUSU MODULAR ANIMAL BEHAVIOR TEST SYSTEM H SERIES USER S GUIDE 99990990990909099909909 59 COPVRIGHT 2001 REVISED 02 25 2003 ETI COULBOURN INSTRUMENTS 5 DEFINITIONS and TERMINOLOGV THE HABITEST SYSTEM CONNECTION and CONTROL THE HABITEST LINC MODULE CURRENT DRAW AND THE MAXIMUM LINC POWER OUTPUT THE ENVIRONMENT CONNECTION BOARD ECB MODULE ELECTRICAL CONNECTIONS SETTING UP THE ISOLATION CUBICLE STIMULUS RESPONSE MODULES GENERAL INFORMATION MODULE SIZES MODULE FIT INSTALLING MODULES STIMULUS MODULES VISUAL STIMULI H11 SERIES STIMULUS MODULES AUDITORY STIMULI H12 SERIES STIMULUS DEVICES ELECTRICAL SHOCK STIMULI H13 SERIES H13 01 2 POLE SHOCKER H13 15 PRECISION REGULATED SHOCKER H13 16 PRECISION REGULATED PROGRAMMABLE SHOCKER STIMULUS MODULES GUSTATORY STIMULI H14 SERIES FEEDER MODULE ELECTRICAL CONNECTIONS H14 03M H14 03R LIQUID DELIVERY RESERVOIR H14 04M H14 04R LIQUID SOLENOID VALVE H14 05M H14 05R LIQUID DIPPER H14 06R COMBINATION DIPPER PELLET TROUGH DIPPER CUPS H14 06 CP series H14 10R GRAIN FEEDER H14 01M H14 01R PELLET DELIVERY TROUGH H14 22M 20 H14 22 45R PELLET FEEDERS STIMULUS MODULES OLFACTORY STIMULI H15 SERIES H15 01M H15 01R OLFACTORY STIMULUS INJECTION MODULE H15 03 OLFACTORY STIMULUS CONTROL H15 20 INLINE OLFACTANT EVAPORATION CHAMBERS RESPONSE SENSORS GENERAL I
3. Modules may be mounted on the cubicle s walls by applying the soft patch to the module s back plate and the hard patch to the rear wall as illustrated or one of the side walls Press the module to the hard patch to mount it on the wall The high attenuation cubicle has strips on the LAG wrap for this purpose STIMULUS RESPONSE MODULES GENERAL IN FORMATION MODULE SIZES There are four heights of module panels one half unit one unit two units and four units in height Each of the S R devices is built on one of the larger three All 4 sizes are also available as blank panels the smallest size is offered as a blank and serves to provide more flexibilitv in fine tuning the vertical spacing of the S R modules There are two widths of each one for mouse cages and one for rat cages The test cages and shuttle cages hold modules totaling eight units of height The hubs and terminator hold modules totaling six units of height 1 2 UNIT SPACER 1 UNIT MODULES 2 UNIT MODULES 4 UNIT MODULES MODULES COME TEST amp SHUTTLE CAGES OTHER ARENAS amp IN 4 SIZES HOLD 8 UNITS TERMINATOR HOLD 6 UNITS MODULE FIT With a few exceptions anv module mav be placed in anv position in the bav and anv module mav be placed in an adjacent bav next to anv other module Excepting a few modules like the runwavs and the Nest Retreat Box all modules structural components are contained within a rectangle defined by the rearwar
4. Programmed Positions Use one of these 3 switch settings when using the shocker in the programmed mode where the protocol determines the level of shock for each presentation Range A program generated 0 to 2 5 Volt control input on the programmed control input on the rear of the case will control the level of shock from 0 milliamps to 5 milliamps Select one of the 3 meter ranges below for ease of reading according to the range of shock level to be used Prog 1 Position Meter reads 0 1000 microamps on upper scale Prog 3 Position Meter reads 0 3 milliamps on lower scale Prog 10 Position Meter scaled 0 10 milliamps on upper scale max output about mid scale Programmable Current Control Setting the output in milliamps directly in the Prog window presented by the Graphic State Notation program when you select the stimuli in protocol creation will produce the zero to 2 5 volt signal from the Habitest Linc s Prog output which will in turn produce a zero to 5 milliamp shock output when it is present You may also use any D A converter that can produce a 0 to 2 5Volt signal if you are using another control system The program control voltage must be present to set the shock level to the selected current value The remote operate input must be activated by an output from one of the Habitest Linc s AUX outputs or other 5 to 30 Volt signal for the shocker output to be on at the selected level To select the value f
5. INFORMATION ON SETTING UP THE LINCS ON THE POWER BASE AND CONNECTING THE SYSTEM TO THE COMPUTER IS FOUND IN THE GRAPHIC STATE NOTATION USERS GUIDE All inputs to the Habitest Linc interface are simple switch closures and all outputs are 28V stimulus control signals You do not have to worry about adding switch input buffers or stimulus output power drivers associated with each stimulus and response device nor any other electronics as you add new S R devices to your system they are already there built into the Habitest Linc interface module Each S R module plugs into an environment connection board ECB which is connected to the Habitest Linc so there is no wiring just slip a module into the wall track and plug its connector into the board see page 10 The special electronics for the more complicated response sensors and stimuli are on the stimulus or response module itself or in a small box in the connector line no special interface modules are needed No special wiring or hookup is necessary just plug them to the ECB in and use them as if they were simple lamps or switches MODULE CURRENT DRAW AND THE MAXIMUM LINC POWER OUTPUT Each Linc can provide Amps of 28VDC to power modules Most modules don t draw enough power to be of concern For example each of the colored lights an LED or Light Emitting Diode on any of the visual stimulus modules draws only about 20 milliamps However there are some modules that have motors like
6. solutions milk and just about everything else Connect the 6 pin connector to a feeder connector on LIQUID LEVEL the environment connection board The feeder coil PHOTOCELL TO DETECT HEAD ENTRY operate signal must be timed by software for the time necessary for the animal to enter the magazine port and lick the liquid from the end of the dipper arm It may be desirable to use the detector heads H20 93 Single Photocell Sensor to detect head entry after turning the feeder coil on and then begin timing after entry to assure consistent availability of the reinforcer When using a dipper in a cage in a cubicle it is convenient to put it on the right hand wall as viewed from the cubicle door as the intelligence wall if only one wall is to be used This will place the reservoir tray such that the tray may be removed from the side you are facing the view in the diagram above 20 DIPPER SPECIFICATIONS Voltage 24 to 30 Volts 28 Volts nominal Coil Operating Current 375 mA at 28 Volts Lamp Operating Current 40 mA at 28 Volts Quiescent Current None Duration Timed for desired access Reservoir Capacitv 50 cc usable H14 06R COMBINATION DIPPER PELLET This module has provision for mounting an H20 93 photo detector to report head entrv The combination dipper and pellet trough is designed for use when 2 levers are used with both pellet and liquid reinforcements It permits the centering of both
7. the feeders retractable levers and guillotine doors that draw significant power though only for a short time when they are actually moving The high current modules have the current draw highlighted next to the name and model number throughout this manual THE ENVIRONMENT CONNECTION BOARD ECB The ECB features an on board house light that operates in parallel with the modular house light If you are using an S R module house light and do not want this one to come on simply remove it A spare bulb is provided in the extra location of the house light group There is also provision for an optional non modular tone device It mounts directly on the board he board mount unit is not as loud as the S R module but it is very economical and adequate for audio cueing modular unit has low and high amplitude settings the latter loud enough for aversive conditioning If you havebeth types connected they will both be on when commanded because there is only one control circuit We ve also provided a constant 28V connector so that any single stimulus module may be constantly powered as long as the Linc or connection panel is powered For example the fan or house light could be on any time the interface is powered You may want to do this to free up one of the spare connectors to power an extra retractable lever tone or other device There are also test points for each and every stimulus device These small circuit pads und
8. AJAX WT BK RD GN VL BL CNTRL1 CNTRL2 CNTRL3 RD GN VL USER DIAGRAMS FOR MODULE FUNCTIONALITY ILLUSTRATED IN THE STATE GRAPHIC RESPONSE SWITCH TRIPLE OR TRI COLOR CUES INPUT CONNECTIONS AS SHOWN IN STATE GRAPHIC 28V SOURCE RESPONSE INPUT IMPORTANT NOTE The H02 08 Linc interface and the new H series Habitest system are not designed to drive heavy loads in S R modules directly The Linc s maximum output current for each command line is 100 milliamps This is adequate to drive all stimulus lights small electromagnetic coils and devices that are active in that they have onboard electronics that derive their main operating power from the 28VDC power bus on the H series ECB Examples of these devices are the Pellet Feeder guillotine doors and the retraction circuit of the Retractable Lever SETTING UP THE ISOLATION CUBICLE ROUTE THE CABLES AS SHOWN THE BLUE LINE AND CLOSE THE COVER INSTALL EACH ECB ON ONE SET OF THE POSTS USING THE THUMBSCREWS INSTALLED ON THE POSTS 4p AT THE FACTORY THE FEET ON THE BOARD WILL NOT BE IN THE WAY SHOCK AUXILLARY AND OTHER SPECIAL CABLES MAY BE PASSED VIA THE CENTER PORT BETWEEN THE ECBS ON THE REAR OF THE CUBICLE REMOVE THE TWO wi THUMBSCREWS FROMTHE _ __ CABLE PORT BOTTOM a COVER PLATE OBSERVE THE KEY ON THE CABLE CONNECTOR IT FACES YOU AND IS UP THEN LIFT THE COVER Cables from the Habitest Linc connect to the Envi
9. can be recorded with a formed meniscus in liquids translucent at 940 nM The drink tube is mounted in a nylon block and is electrically isolated from the rest of the module Aversive shock may be delivered through the drink tube for the Vogel procedure shock lick suppression without electrifying the module face or the rest of the cage Use one of the two pole shock cables discussed in the shocker section that has the clips on the end STOPPER FITS GRADUATED Connect one pole to the tube and one to the floor CYEINDER OR BOTTLE with the shorting block provided with the cable Licks be measured while shock anv electrical stimulus is being delivered without spurious current paths or interference MOUNTING THE PHOTO DETECTOR ON THE LICKOMETER BODV SHOCK CABLE 5 TO SCREW SHANK Refer to page 28 for an illustration of the general mounting configuration For this unit LOOSEN SCREW TO REMOVE TUBE AND BOTTLE OPTICAL AXIS in the mounting bracket After mounting the bracket tighten the screw slightly and rotate the plate to align it to center the U slot over the glass rod Install the head in the U slot sliding it fully tothe Then tighten the screw firmly DRIBBLE COLLECTOR being careful to not change the alignment 30 H24 01 TB 01 SINGLE LUMEN CONTROLLED FLOW DELIVERV TUBE H24 01 TB 03 TRIPLE LUMEN CONTROLLED FLOW DELIVERV TUBE These
10. chemical screens are provided to ensure product consistency Test substances can be added and you can choose from a wide variety of custom flavors colors and formulations In the interest of freshness Coulbourn Instruments does not stock Bio Serv products To order please contact Bio Serv directly 23 STIMULUS MODULES OLFACTORY STIMULI H15 SERIES All connections for items on this page are push on barbed nylon fittings for 1 8 inch inside diameter tubing GAS FROM CONTROL H15 01M H15 01R OLFACTORY STIMULUS INJECTION MODULE This module consists of a module plate with a small flush mount fitting that accepts the tubing from the H15 03 on the rear of the module and allows gases to pass into the animal s working area It can be mounted in the bottom module position below the floor plane or in any other position Use an H29 05R Fan Module page 35 for exhausting arenas 4 15 01 OR 01R MODULE PLATE OUT TO 3 DIFFERENT LOCATIONS H15 03 OLFACTORV STIMULUS CONTROL This unit can also be used to deliver aversive air puffs into up to three locations from a common supplv The H15 03 contains a 3 unit solenoid valve manifold that permits individually gating or mixing up to 3 gasses or 2 gasses plus flushing air Low dead space in the manifold means minimum mixing when changing from one line to another permitting rapid flushing The manifold is machined from Teflon The solenoids are operated by a
11. is clear of the trough and the hopper is balanced properly GRAIN FEEDER SPECIFICATIONS Voltage 24 to 30 Volts 28 Volts nominal Coil Operating Current 750 milliamps at 28 Volts Lamp Operating Current 40 milliamps at 28 Volts Quiescent Current None Duration User controlled for desired access time Hopper Capacity 230 cc H14 01M H14 01R PELLET DELIVERY TROUGH This module has provision for mounting an H20 93 photo detector to report head entry or pellet removal for automatic take and FEEDER monitoring DETECT HEAD ENTRY LIFT HOPPER UP AND OUT TO CLEAN amp FILL HOLE FOR PHOTOCELL DETECTION OF HEAD ENTRY PELLET FROM FEEDER PELLET BLOCKS DETECTING PELLET REMOVAL The pellet trough is a recessed cup baffle and chute which receives the pellet feeder delivery spout The access port for the rat model is 1 3 8 inches wide by 1 5 8 inches high Opening dimensions for the mouse model are proportionally smaller The ports for both are 1 deep with a DETECT PELLET REMOVAL depression in the center where the pellet comes to rest for easiest access by the subject A magazine light is installed inside the top of the opening The unit has two locations to mount the photo detector of the H20 93 one to detect entry of the subject s head and the other to detect pellet removal When a pellet is removed the detector reports the event for recording and rep
12. per second up to a maximum of 50 per second This is an ideal method of bringing magnitude data into a behavioral software package which is after all event rate oriented for contingency structures The output pulses represent a number of Newton seconds of force applied or other time integrated units for other transducers USE ANY HABITEST LOAD CELL PLATFORM WITH ANIMAL HOLDER SHOWN CLIMBING CAGE OR LIVING CAGE FOR MEASURING ERGOMETRIC ACTIVITY STARTLE OR TREMORS ALSO STRAINGAGE BRIDGE TRANSDUCER FROM OUR V SERIES SVSTEM OR FROM ANOTHER MANUFACTURER F COULBOURN TRANSDUCER MONITOR BALANCED AC HIGH TRESHOLD R TOR BALANCE LOW PROPORTIONAL POWER COUPLING SENSITIVITY THRESHOLD ADJUST OUTPUTS SWITCH THRESHOLD B SWITCH PROPORTIONAL 38 24 72 RESISTIVE BRIDGE TRANSDUCER MONITOR Specifications Common Mode Rejection 100 dB Min DC 60 Noise RTI 1 Hz 1000 Hz 0 4 Bridge Excitation 5 0 Volts Coupling Frequency Response DC Coupled DC 1500 Hz Coupled 1 Hz 1500 Hz 3 dB Balance Control Auto Balance Pressing down initiates autobalance Balance Precision 32 mV of center of 5 V range Balanced LED Indicates successful autobalance Sensitivity Control 3 Ranges Accuracy 2 Digital outputs Switch closures to 28V ANALOG RESPONSE SENSING NOTES V THRESHOLD SETTING ov LOGIC 1 ON ke
13. shocker to ON OPERATION Remote Manual Operate Up Enables an external signal to operate the shocker Center Disables remote operate Down Operates shocker manually for duration of depression The down position of the switch is spring loaded to return to center when released 13 Remote Operate On the back panel turns shocker on when external voltage is applied 5 to 30 VDC at 20 milliamps In our Habitest system use an auxiliary output and follow the color code blue to blue 28 V command and black to black common ground In the Graphic State program select the Aux output in the state you want to present shock When the shocker is wired for remote operation you may prevent it from being operated manual override of remote signals by placing the OPERATE toggle switch on the front of the H13 01 to the OFF position Shock Output 2 terminal posts on the rear of the cabinet Connect the shock cables to these posts and then to the grid floor drink tube tail shock apparatus etc CABLES FOR H13 01 SHOCKER H93 07 25 2 CONDUCTOR CABLE FOR ALTERNATE GRIDS 25 FT H93 07 50 2 CONDUCTOR CABLE FOR ALTERNATE GRIDS 50 FT FOR ALTERNATE GRID 2 POLE SHOCK TO GRID FLOOR OF CAGES OR 4 TO H93 30 FOR HUBS 25 OR 50 FT H93 09 25 2 CONDUCTOR CABLE PIN TO CLIP 25 FT H93 09 50 2 CONDUCTOR CABLE PIN TO CLIP 50 FROM SHOCKER OUTPUT FLOOR ra ONE OBJECT H13 15 PRECISION
14. 1 2 O O 1 5KHz e Om 2KHz 9dB O O O Om 2 5KHz 12dB GOO Om 3KHz 15dB OO MI i EPEREA 3 5Khz 18dB e 4KHz 21 12 STIMULUS DEVICES ELECTRICAL SHOCK STIMULI H13 SERIES Resistance regulated The H13 01 is an economical series resistance regulated shocker for small animals The set accuracv is 5 and the regulation is 20 over a 10K to 30K ohm subject resistance range It is fully isolated for subject and operator safety using the same shielded and grounded transformer as our feedback regulated research model H13 16 below The operate control input is optically isolated to simplify operation from any signal source from 5 to 30 VDC It is designed for student lab applications and other protocols where precise current control over wide ranges of subject resistance is not required Series resistance regulated shockers can never deliver more current than the total circuit resistance predicates The current output IS therefore inherently overshoot limited and the shocker may be used in applications where continuity is broken during shock administration This means that it may be used with small animals in any attached or unattached electrode two pole applications as well as with test cage grid floors using alternate grid bar connections where precise stimulus parameters are not required However it should be noted that it is possible for anim
15. 19 50 2 CONDUCTOR CABLE PIN TO CLIP 50 PROM al OUTPUT TO ANY TWO OBJECTS 447777 gr 25 OR 50 FT FLOOR CAN BE Pa ONE OBJECT ARENA AND RUNWAY SHOCK CONNECTIONS FROM SHOCKER TO CAGE OR DISTRIBUTOR BOARD SHOCKER E MCOULBOURN ANY CAGE FLOOR OUT RUNWAY CONNECTOR os OUT TO ANY CAGE OR ANOTHER DISTRIBUTOR t NOTE RUNWAYS HAVE A CONNECTOR ON EACH SIDE TO ALLOW DAISY CHAIN CONNECTIONS FROM RUNWAY TO RUNWAY USING ONLY H93 31 32 SHOCK CABLES 18 STIMULUS MODULES GUSTATORY STIMULI H14 SERIES FEEDER MODULE ELECTRICAL CONNECTIONS All feeders in the Habitest system are operated via the same two pins in the connector The magazine light is operated by the same pin that controls the i red cue light and the mechanism that controls the food or liquid delivery MAGAZINE presentation is operated bv the same pin that controls the green light The LIGHT diagram to the right illustrates this and makes it obvious that anv feeder can be operated a connector on the in the verv unlikelv event that vou must control more than 4 feeders from a single Linc Conversiv and more likelv vou mav control the red and green lights or a corresponding pattern or cue function bv using the Feeder connectors NOTE In the state graphic window in the Graphic State Notat
16. 3 pellets requires 1 68 seconds etc The number of pellets in each slew or burst operation of this type must not exceed 25 with at least a 10 second rest off time before the next burst PELLET FEEDER SPECIFICATIONS Voltage 24 to 30 Volts 28 Volts nominal Motor Operating Current 495 milliamps at 28 Volts Quiescent Current 85 milliamps at 28 Volts Lamp Operating Current 40 milliamps at 28 Volts Duration 1 millisecond for a single pellet delivery Motor Frequency 200 Hz Hopper Capacity 2000 minimum 45 mg For lamp in trough or in combination dipper trough NOTE ROUTINE CLEANING OF PELLET DUST The feeder must be empty before cleaning Remove the motor plate mounting screws on the plate on either side of the motor that attach it to the top plate of the hopper Lift the motor assembly from the hopper Clean the hopper and disk with a small brush Reinstall the motor assembly on the same hopper if you are cleaning many units at the same time making sure the pellet occluder post is toward the front panel DUSTLESS PRECISION PELLETS Bio Serv One 8 Street Suite 1 Frenchtown NJ 08825 TEL 908 996 2155 FAX 908 996 4123 Bio Serv s Dustless Precision Pellets are nutritionally balanced and virtually dust free resulting in hassle free performance in Cl feeders They are available in both grain based and purified formulations and in banana and chocolate flavors in both 20 and 40mg sizes Nutritional assays and
17. LLATION H10 37R GDM H10 37M GDM FINGER HOLE SAFTEY SLOT ue 4 10 37 PROG SLIDE THE LIFTER PIN THROUGH THE FINGER HOLE IN THE DOOR PLATE BEFORE INSTALLING THE THUMBSCREWS 36 ACCESSORIES THE HABITEST amp TRU SCAN SVSTEMS A21 10 LIGHT INTENSITV AND COLOR CONTROLLER This device may be used with any lamp LED or incandescent rated up to 1 Amp in the Habitest system It connects to any TRIPLE CUE or a SPARE single cue module at the environment see pages 7 amp 11 Itis controlled by a spare output to provide 28 Volts and an analog output to provide the proportional signal from the back of the Linc It can also be used to control magazine lights The 1 Amp 28 Volt signal provides a reference for a power driver which is driven by a SVCOMSOURN MABITEST retriggerable one shot driven in turn by a 421 40 LIGHT INTENSITY CONTROLLER voltage controlled oscillator The net result is that the higher the voltage is on the analog input ae the faster the one shot fires Since these things O E happen far faster than flicker fusion and the period of the one shot is fixed lamp LED incandescent driven via a driver the one ANALOG INPUT ECR shot changes in brightness as the frequency oes 9 The center knob simplv controls the routing of the signals The first 3 positions route the signal f
18. NFORMATION RESPONSE SENSORS BUILT INTO ARENAS H10 11M PA MOUSE PASSIVE AVOIDANCE CAGE H10 11M SC MOUSE SHUTTLE CAGE H10 11R PA RAT PASSIVE AVOIDANCE CAGE H10 11R SC RAT SHUTTLE CAGE H10 37R LD COUPLER W LOCKABLE DOOR H10 38R SHOCKABLE RUNNING WHEEL Q A 69 000 22 22 23 24 24 24 24 26 26 26 26 26 26 27 27 5 RESPONSE SENSING MODULES H10 39R NEST RETREAT BOX H20 93 SINGLE PHOTOCELL SENSOR H20 95 PHOTOCELL SENSOR BAR H21 03M H21 03R RESPONSE LEVER H21 05R OMNIDIRECTIONAL RESPONSE LEVER H21 09M H21 09R NOSE POKE OPERANDUM H21 15R BIRD PECKING KEV H21 17R KEY W 3 COLOR CUE H21 19R KEY W 7 PATTERN DISPLAY H23 17M H23 17R RETRACTABLE LEVER H24 01M H24 01R OPTICAL LICKOMETER H24 01 TB 01 SINGLE LUMEN CONTROLLED FLOW DELIVERY TUBE H24 01 TB 03 TRIPLE LUMEN CONTROLLED FLOW DELIVERY TUBE H24 01M H24 01R OPTICAL LICKOMETER APPLICATION NOTES H24 05 CONTACT SENSOR H24 61 H24 61 MC INFRARED ACTIVITY MONITOR CEILING MOUNT H25 01 CEILING CHAIN H25 06 UNIVERSAL CEILING MOUNT BUMP ROD ACCESSORY MODULES H21 01 HAND SHAPING SWITCH H21 02R H21 02M EXPERIMENTER S MANUAL SWITCH MODULE H29 01 BALANCE ARM MODULE H29 05R H29 05M FAN MODULE H10 37R H10 37M RUNWAY GUILLOTINE DOORS H10 37 RUNWAY GUILLOTINE DOOR LIFTER ACCESSORIES TO THE HABITEST SYSTEM A21 10 LIGHT INTENSITY AND COLO
19. R CONTROLLER A24 72 RESISTIVE BRIDGE TRANSDUCER MONITOR ANALOG RESPONSE SENSING APPLICATION NOTES A28 21 MOTOR SPEED CONTROLLER 27 27 28 29 29 29 29 DEFINITIONS and TERMINOLOGV ARENA Arenas are the confining space in which the animal works including cages and hubs that have tracks to hold stimulus presentation and response sensing modules the computer uses to interact with the subject CHAMBER total experimental environment consisting of a test arena above placed inside of an optional isolation cubicle below to block extraneous stimuli when and where necessary CUBICLE An optional enclosure into which to place a test arena in order to block visual stimuli and to attenuate auditory stimuli ECB See Environment Connection Board ENVIRONMENT The total environment includes arenas with stimulus and response modules runways hide boxes running wheels and cubicles in which arenas are placed ENVIRONMENT CONNECTION BOARD or ECB The printed circuit board in the environment to which the stimulus and response modules are connected This board connects to the Habitest Linc via multi conductor ribbon cables The Linc receives switch input signals representing responses and sends output signals to turn on stimuli via the cables and board to the connectors on the S R modules EVENTS Switch events or response input events to the ECB and the Habitest Linc and ultimately the Graphic State Notation program are
20. REGULATED SHOCKER META A A With grid floor scanner TWO 5 H13 16 REV A PRECISION REGULATED PROGRAMMABLE SHOCKER With grid floor scanner and programmable shock levels These 2 model numbers are based on the same basic shocker The H13 16 is simplv a H13 15 with a programmable shock level control added specifications and operation are otherwise the same The shocker is totallv isolated floating output for operator and subject safetv as well as isolation from other sources of electrical stimulation such as phvsiological stimulators that mav be in use concurrentiv The unit can function as a two pole bipolar current reversal square output or as a scanner distributing the polaritv reversal across an 8 pole output for grid floor applications Control of the mode is bv a switch on the back panel The grid outputs are direct Form C semiconductor switches The off grids have zero resistance so the subject receives the same current regardless of the number of grids contacted The H13 16 mav also be programmed from 0 to 5 milliamps in 20 microamp increments bv a 0 2 5 Volt signal via the PROG INPUT on the back of the case The signal is generated bv the Graphic State Notation 14 control program and comes from the PROG OUTPUT on the back the Habitest Linc you may also use your own D A converter output Specifications pertaining to the H13 16 only are in red type With the Two Pole Scan
21. a pulsed switch closure at the onset of a beam break for licks using the optical lickometer or a continuous switch closure for the entire duration of the break to sense animal position Vou mav also select a delaved opening to detect a pellet removal while ignoring bounce upon initial deliverv The light source and photodetector cell operate in the invisible infrared region 940nM and each is enclosed in a small grooved cylindrical head which mounts on the following devices H10 37R XXX All Runways H24 01MorR_ Optical Lickometers H14 01MorR Pellet Troughs H14 05M Liquid Dippers H14 06R Dipper Trough H14 10R Grain Feeder NOTE The H20 93 is part of the H21 09M 4 098 Nose Poke modules and need not be ordered separately MODULE WALL lt _ RETAINING PLATE SLIDE THE LIGHT EMITTING AND PHOTODETECTOR HEADS ONTO THE RETAINING PLATES D INSTALLATION AS TRANSIT DETECTORS ON RUNWAYS lt DETECTOR OR EMITTER HEAD SCREW SPACER WASHER You may use photobeam detectors to detect entry or passage into arunway To do this place the screw supplied with the sensor head through the runway wall from the inside Then from the outside place the spacer over the screw followed by the lock washer the retaining plate and finally the nut Tighten the nut Slip the light source head into the slot on the bracket on one side and the sensor head into the bracket across the runway from t
22. al box runway as a start box to permit the animal to be introduced to the environment for a waiting or adaptation period and then be released into the main working environment without handling variables when the experiment starts The manual doors may be lifted automatically by the optional universal door lifting mechanism H10 37 below The lifter may be added at any time to automatically lift doors that you have been lifting manually without any change in the environment from the animal s perspective H10 37 RUNWAY GUILLOTINE DOOR LIFTER This device may be mounted on any rat or mouse runway to lift an H10 37R GDM or H10 37M GDM Manual Guillotine Door above It mounts with two thumbscrews on the bridge bar at either or both end s of any runway You can add the lifter at any time after you purchase the manual door A lifting pin fits into the hole in the door to lift it but when the door pin goes down the door will fall freely The slotted bottom of the hole allows enough free travel for the door to rest on the animal s tail or back as a safety measure It will then fall closed when the animal is clear The lifter may be programmed by any spare or other stimulus output see page 11 When the stimulus is on the door is in the up position By placing the switch on the side of the case in the UP position the door will not respond to programmed outputs and will remain in the up or open position RUNWAY GUILLOTINE DOOR LIFTER INSTA
23. alligator clip attached to the tube retaining thumbscrew and the other pole to the shorted grid bars of the floor See shock cables on page 14 H93 09 25 or 50 and 18 h93 19 25 or 50 Licks may be measured while tongue or foot shock or any other electrical stimulation is being delivered without Spurious current paths or interference because the response is measured optically The tube holding block and indexing collar are factory adjusted to place the tip in position relative to the light path for best performance of lick sensing with rats and mice The collar on the tube is locked in place so that the bottle and tube may be returned to the same position each time it is removed for filling IT SHOULD NOT BE REMOVED The tube with the attached stopper and bottle are retained by the tube retaining thumbscrew in the plastic block remove the assembly loosen the knurled thumbscrew Remove the stopper and tube assembly from the bottle and fill the bottle Then replace the assembly making sure that the locking collar is fully against the Delrin block to properly index the tip of the tube Then re tighten the thumbscrew The tip of the drink tube will be in the same position even if the stopper is neither on the tube nor in the bottle in the same position Adjustment is possible in case you are using other species with grossly different response topographies It is accomplished in two dimensions One dimension controls the depth o
24. als to avoid being shocked by learning to stand on alternate grids since grids do not change their relative polarity as they do in scanned output shockers H93 07 25 and H93 07 50 shock cables have two pins on the shocker end for this shocker and jumpered 8 pin connectors for alternate grid connection on the other See next page SPECIFICATIONS Power On Off Switch On front panel Remote Manual Operate Remote Up enables external operate Off Center disables remote operate Manual Down operates shocker manually Remote Operate Turns shocker on when external voltage is applied Set Current Sets current from 05 milliamp to 2 0 milliamp Shock Light On for duration of operation Number of Outputs 2 Maximum Current 2 milliamps into 10K Ohms Maximum Voltage Output 280 VAC 60 Hz Waveform 50 or 60 Hz sine depending upon line mains frequency Regulation 20 over 10 to 30K Ohms Dimensions 3 1 4 in Hx 10 W x 7 D Electrical 110 120 VAC 50 60 Hz or 220 240 VAC 50 60 Hz specify on order INSTALLATION 1 Attach the AC mains power cord to the AC input on the rear of the shocker and plug the cord into an appropriate AC mains outlet 2 Attach the electrodes or shock cable to the binding posts on the rear of the shocker 3 Set the desired shock amplitude by adjusting the SET SHOCK LEVEL on the front of the shocker Range 50 microamps to 2 milliamps 4 Turn the AC power switch on the front of the
25. and data acquisition purposes selector switch permits the user to choose either of two output modes Vou mav choose movement units brief pulses representing the minimum resolution of detection or movement episodes a continuous output for the entire duration that movement units occur with inter event intervals of less than 400 milliseconds ACTIVITV MONITOR The dual element differential detector senses the emitted infrared bodv heat image of the z subject 13nM infrared radiation through arrav of lens facets that create a field of multiple images on the two detector elements Any relative change in the energy falling on the elements is defined as a movement unit and results in a 12 ms 28V pulse output closure in the unit mode at the output to be routed to the Linc via a Switch connector on the ECB This design gives excellent sensitivity linearity and inter unit consistency because a variable pulse rate where each pulse represents a uniformly time space defined movement unit produces the magnitude measurement pulses per unit of time The clear plastic Vivak comprising the cage walls is opaque to 13nM radiation Therefore interference from outside moving heat sources e g the experimenter is not a problem All electronics are on board so that the unit connects to a standard Switch connector of the ECB Installation and Operation The H24 61 is supplied with two th
26. ble to the standard H21 03R lever H21 09M H21 09R NOSE POKE OPERANDUM The electronics of the H20 93 SINGLE PHOTOCELL SENSOR above are built into the module Response detection is by the invisible 940 nM infrared photo beam across the opening of the hole at the front of the cylinder The printed circuit board on the module has all of the features provided in the H20 93 A 28 Volt signal is provided to report nose entry to any Switch input of the ECB Both modules have red amber and green LED cue lights that are mounted on the rear of the cylindrical chamber behind the key They are operated by a Cue output of the ECB The inside of the chamber is white plastic to scatter the cue light and make it visible from a side angle view A gaseous olfactory stimulus connection is affixed to the chamber to receive tubing from the H15 03 Olfactory Stimulus Control H21 15R BIRD PECKING KEY H21 17R KEY W 3 COLOR CUE H21 19R KEY W 7 PATTERN DISPLAY These response keys are fabricated from transparent plastic and fitted with travel adjustment screws The same module panel is used for all 3 numbers listed A rear projection screen backs the H21 15R key paddle The switch is a sealed highly reliable microswitch type The minimum force required to actuate all keys is 13 grams The tricolor cue lights and 7 pattern display lights shown both activate on 28 z V from output Refer
27. cca M OI LL THRESHOLD COMPARATOR MODE V LOGIC 1 ON oy LOGIC O OFF LI PROPORTIONAL TIME INTEGRATED SERIAL PULSE ANALOG CONVERTING ANALOG SIGNALS TO EVENTS Some of our behavioral response sensors as well as other transducers provide information about the analog magnitude of certain tvpes of behaviors The A24 72 Transducer Monitor reduces that analog information to a series of pulses that varv rate so that thev be handled the interface as event like inputs The pulses represent units of behavior and are handled the same wav as if thev were discrete events like those defined bv the binarv on off nature of a response device like a lever Discrete on off events specificallv lever presses runwav entries nose pokes etc are just like the analog threshold events in the upper trace of the figure on top of the page Circuits which detect the level magnitude or amplitude of an analog cursive or proportional signal are variously known as comparators level detectors Schmitt triggers or threshold detectors These events are just like lever press events except that the Transducer Coupler threshold function defines the switching point rather than the spring and metal contacts of a lever or the breakage of a photobeam The comparator function of the A24 72 Transducer Monitor will detect the point at which an analog signal from a transducer exceeds a given us
28. cks must stop for at least some brief time before the pump will be turned off You as the experimenter must determine which method is appropriate for your protocol as well as the drive rates and syringe size Among the things to consider are concepts such as intermittent reinforcement where each lick is an operant and only lick results in a drive pulse of sufficient duration to deliver a quantity of water to be ingested in a single lick Another protocol may embody the concept of cued availability of free access drinking for some period or periods of time in the experiment run and employ either the lick pulse mode or constant drive episode mode The dribble collector is generally not necessary for rats and mice as these species spill little or no liquid from this device but it is a simple matter to place a jar lid under it to be sure H24 05 CONTACT SENSOR For both rat amp mouse EVCOUSOUN The contact sensor is an electronic switch HAL that senses the passage of a low current than 5 microamps and is well below sensation threshold The standard response sensor connector on the unit connects to anv mar nie through the subject The current level is less switch input on the ECB just as do levers etc SWFIEH GW ELE The sensing leads connected to anv two conducting parts of the environment When the subject touches both completing contact the swi
29. d projection of the perimeter of the module face outline Restrictions on placement apply only to the 6 bay test cages because the 3 bays on each side of the cage are in a flat planar array Any module will fit next to any other in the radial array of any hub where the bay tracks are wider and the modules angle away from each other creating even more clearance INSTALLING MODULES To install modules remove the cover plates of the 6 bav test cage or the lid of the hubs Just slip the modules down into the grooves of the tracks that form each module bay You may use any size blank module panel metal or clear plastic to space modules at the desired height from the floor or from the module below After the module is installed connect it to the ECB in an appropriately named connector i To place a terminator at the end of a runway place the runway along with any module or blank panel that you want at the end of the runway in the double tracks of the terminator Then to make it easy to install the whole runwav arm lift the entire assembiv terminator modules and all and slide the other end of the runwav into the tracks of the cage or hub If you are using a module with a part that protrudes from the front panel like the paddle on the lever shown below place the module and the runway end plate together face to face with the protrusion fitting into the open space at the end of the runway Then hold them together and place both into the t
30. e other of the elements Each image s slightly different displacement increases the relative change caused by a subject movement The sensor s output signal is amplified and used to trigger a movement unit pulse for each edge transition Since the lenses are Fresnel type and have a non critical focus relatively great depth of field the unit may also be used in large area applications including open field mazes primate chairs and cages and even room sized fields with humans NOTE A movement unit is dependent upon image size and distance of the movement The time distance constant depends on viewing distance and the actual size of the subject image area on the sensor so an actual spatial velocity specification is of little use This also means that data for grossly different sized or species of animals is not comparable The H24 61 monitors movement in the x y and z axes Z axis movement is detected both because any movement up a wall is seen as radial movement and because central vertical movement is seen as a change in size It will detect rearing of mice with the optional suspended ceiling kit H24 61MC and some smaller movements grooming etc of rats The maximum rate of movement unit pulses cannot exceed 50 pulses per second A rapidly moving animal making very large movements can produce a pulse rate of up to 15 or 20 pulses per second Even at these rates Graphic State software can handle 16 stations simultaneously along wit
31. e arm For larger than this standard volume the interchangeable cups may be ordered and attached to the end of the arm They slip over the end of the arm and are held in place by a small recessed set screw The cup and screw are stainless steel An Allen wrench is provided with the cups It is difficult to align the arm if it is removed it s a 3 handed job for the inexperienced 21 H14 10R GRAIN FEEDER 750 This module has provision for mounting an H20 93 photo detector to report head entrv The Grain Feeder is capable of delivering almost anv drv food of small granular structure It is commonly used for bird seed or extruded pelletized feed commonly formulated for pigeons It may also be used to deliver small pieces of other dried feeds such as shrimp To operate the feeder connect it to one of the Feeder connectors on the ECB and time the operate signal allowing sufficient time for the animal to consume the desired amount of food You may find it behaviorally advantageous to mount detector heads on the unit to permit bringing up the feeder with an operate and starting a timed interval upon head entry to delay the drop to allow the desired access time after head entry It can be used for monitoring ad lib feeding when used with the photo detector The entire food hopper lifts off for filling and cleaning Simply lift up until the pivot pin clears the pivot groove in the arm Then move it rearward so that the bottom
32. e stimulus modules the Board Mount the High Power modular tvpes and the in line Noise Generator are operated via anv one of the Spare connectors or the Green control line of anv one of the triple Cue connectors See page 7 The in line Tone Generator board and the in line Attenuator board are operated to their full capabilitv bv using a Cue connector Select the frequency or attenuation level in accordance with the color codes shown page 7 and below in conjunction with selection of the matching color in the state graphic Either may be operated via a Spare connector but the Tone generator can produce only a 1KHz tone and the attenuator can produce only 3dB of attenuation The case for all 3 auditory modules is shown at bottom see 8 The speaker may receive signals from the audio pass through on the ECB which passes nominal 1Volt RMS audio signals via the jack on the back of the Linc from an outside source The speaker is also used to reproduce the tone signal from the Tone Generator by connecting it directly to the output of the Tone Generator The signals from the Tone Generator Noise Generator or the pass through jack on the ECB may be routed to the speaker via the Attenuator in series to afford program control over the amplitude of the signal MABITEST 17 88 i xiri TONE ATTENUATOR TRIPLE CUE FEEDER SPARE TONE GENERATOR HOUSELITE OFF NONE
33. e transducer model number the units of measure desired and the sensitivitv setting on the monitor When vou create a data analvsis element the data presented in vour event lists and graphs will have the selected datum name and will be automaticallv scaled for the sensitivitv and range of the transducer and the setting on the interface monitor Your event graphs will bear the datum name you selected activitv etc with the associated proportional units of measure Newton seconds etc Both our E24 72 Transducer monitor and our E24 61 Ceiling Mount Activity Monitor are capable of pulse rates well above 10 per second The Transducer monitor is capable of rates up to 50 per second A28 21 MOTOR SPEED CONTROLLER This device was originally developed to control the speed of our Roto Rod but will be used for other motors in the future and become a general purpose motor control as time goes on may be used for any one of the three speeds available in the Roto Rod motor according to the chart below The spare input is the pickup for the 28 VDC that drives the motor The analog lead that drives the control input is provided with the unit and is connected to the analog output on the back of the Linc The motor control lead on the motor is connected to the jack labeled MOTOR OUTPUT on the right of the unit CVCOUBSOURN MABITEST 28 21 MOTOR SPEED CONTADLLER o PROM LINE SPE CF OUTPUT
34. ect Routes shock to the subject output cable in this position The yellow light indicates that shock is being routed to subject VERY IMPORTANT NOTE WHAT DOES THE METER READING REALLY MEAN When setting the shock output the routing switch is in the set position and the current level is set while reading the meter The unpulsed shock source is fed directly to the meter which measures current constanily applied to the test set load When the shocker is in the use mode routing to subject the level of current during the pulse on portion of the cycle is the same as was originally set or programmed in the set mode See above for meter ranging 16 When the shocker is in use with a subject the current is off one third of the time across given pair of grids Thus the meter reading when in use with a subject is lower than when it was set due to its mechanical integrating properties but the current at anv instant when it is flowing is exactiv as it was set or is programmed Also consider the fact that the animal or may not be contacting the grids at any given moment reducing even further the average reading in the use mode For example if the rat is standing on two grids current is only flowing two ninths times one third of the time If the rat is jumping at random intervals and landing for some unknown fraction of time on an unpredictable number of grids then one cannot expect the meter to indicate any more than a general ran
35. er The 3 valved ports connect with a 4th non valved port in a common verv low volume chamber Gases mav flow in either direction Vou mav take 3 gas sources into the 3 valved connections and route a selected single gas or a mixture of the 3 out of the 4th connection There are of course 3 single gases and 4 combinations of gases that mav be routed to a single location See page 12 for the use of binarv color codes for the state graphic in the software that controls the device When using gases as discriminanda in the select mix mode generally one of the 3 is plain air for flushing the common chamber connection lines and presentation devices like the nose poke module or the entire arena if vou are using the injection module plate The Olfactorv Injection Module plate is on a single unit height module plate so that it mav be mounted in the bottom position below the floor so that it does not use up valuable S R module working area below a lever for example Gases mav be routed to either Nose Poke module to present them in a confined area so that the animal must make response spatially localized with the stimulus may also be used to fill a runway and make a task where the animal must track an olfactant to the source and confirm his tracking by making beam breaking nose poke an operant response 25 RESPONSE SENSORS GENERAL INFORMATION INPUT EVENTS FOR THE ECB AND GRAPHIC STATE NOTATION All response detecti
36. er each connector are in electrical parallel with each stimulus or response device connected to the connector and labeled in smaller type SWITCH FEED MAG magazine lite CUES Y G TONE etc There is also a connector on the top of the board labeled Probe The test probe one is provided with each System Power Base is attached via a short wire to its connector so that you can plug it in and touch the probe tip to any of the pads to test the stimulus device or response input This can save a lot of time in testing modules because you don t have to run a program to turn them on The two blue LEDs at the bottom indicate to which half of the Linc the board is connected 1 light A 2 lights B The speaker connector is connected via 2 wires in the ECB cable to the same type of audio jack on the rear of the Linc module MODULE ELECTRICAL Each module connects to the via a telephone tvpe connector on the end of the small cable connected to the module For those who wish to connect other S R devices to our svstem or ours to other svstems the technicians diagrams are shown at the top The user diagrams below simply show functionality in a convenient spatial lavout to visuallv separate power the switch connection and the 3 stimulus control lines TECHNICIAN S DIAGRAMS FOR MODULE WIRING CONNECTIONS AT THE PLUG END WIRE COLOR CODES BL BK WT 28V RESP IN COM IJLILI
37. er set level and trigger giving a signal which is on above the setpoint or off below the setpoint This type of circuit is actually a one bit analog to digital converter The output is reoresented as a single bit on a single wire rather than as a multi bit binary code using a greater number of wires This function will convert an analog signal to a discrete input event for the Linc interface 39 The user selectable analog level is part of the event definition somewhat analogous to tightening a spring to increase the force required to trip the switch in a lever The threshold function allows vou to use Graphic State to record the number of times the signal which mav represent force temperature acceleration etc went above a value of vour choosing Either tvpe of event the mechanical threshold of a switch closure or an electronicallv set threshold point on an analog signal is a discrete event you have an analog signal that you wish to record with more precision or resolution than simply as an above or below setpoint event you may easily do so with the monitor Such measures are made by using a time integrated unit of an analog proportional measure as is illustrated in the lower trace of the figure on the previous page and in the figure below Doing this you can easily measure the magnitude of analog signals as event pulses in an event based behavioral control system such as Habitest This method of analog to digital c
38. f the drink tube protrusion past the Delrin isolation block The other rotation controls the radial position of the tube tip determined by the angle of the block see the side view illustration The rotational aspect is locked by means of the two holding screws on either side of the block Depth is adjusted by means of the locking collar that mounts on the drink tube The collar is locked into position with a set screw For feeding and drinking applications this device is less expensive than a dipper but it can only be used when the protocol allows for free liquid access for the entire session unless one of the controlled flow tubes 31 above is used conjunction with an infusion pump Use of the infusion pump allows controlled deliverv of liquid that can be brought under program control as a function of licking as an operant This tube is purchased as an extra rather than as a replacement for the standard tube This is so that animals can learn to drink from the device using the standard free deliverv tube supplied with the unit without the necessitv of using an elaborate protocol involving a pump The control of the pump may be on a lick pulse command basis where each lick results a very brief command to the pump The pump may also be controlled on an episode drive basis where the pump is turned on continuously for a licking episode where the episode definition is no more than T elapsed time between licks In other words the li
39. gar it must be flushed with water after use The fluid must be fed from a gravitv feed supplv such as the bottle supplied with the unit or a reduced pressure feed line Never attempt to connect the feed tube to a mains fed faucet the pressure is too great for the connections Turning down the flow rate from a main line supplv will not reduce the pressure When using the bottle supplied with the unit place the bottle above the module Connect the 6 pin connector to a feeder connector on the ECB To set up a delivery quantity you must adjust flow rate and time of actuation of the valve The slower the flow rate the longer the valve must be open to deliver the same quantitv Adjust the flow rate with the hose clamp provided with the unit Then set vour software to deliver the desired volume bv adjusting the feeder command time O ee SOLENOID VALVE SPECIFICATIONS Voltage 24 to 30 Volts 28 Volts nominal Coil Operating Current 400 mA at 28 Volts Lamp Operating Current 40 mA at 28 Volts Quiescent Current None Duration Timed for desired access Reservoir Bottle Capacitv 1 Liter DONOT OIL THE DASHPOT H14 05M H14 05R LIQUID DIPPER 375 This module has provision for mounting an H20 93 photo detector to report head entrv Either the Liquid Dipper or the Combination Dipper Pellet MANN Trough may be used with almost any fluid of appropriate viscosity It works well with water alcohol sugar
40. ge of average time density current The meter will simply jitter around a reading which is a fraction of the set value Remember the meter is used to precisely set or test the magnitude of the pulsed stimulus current as it will be delivered to the subject It is impossible or very expensive to measure stimulus administration as it is delivered unless attached cutaneous electrodes are used a restrained subject In spite of all of the variables aversiveness is highly correlated with instantaneous current the pulse on cycle which is the value the meter reads in the set test mode Use the meter to manually set or to test the current provided by a programmed level code In the use mode the meter will verify that the stimulus is reaching the animal With no animal in the circuit the meter should show little or no movement If it does you have a short or a resistive path in the cage Check the floor for droppings bridging the grids or moist debris on the grid floor circuit board Very close inspection of the meter may reveal a very slight trepidation of the meter movement within open circuit clean cage This is due to the resistive equivalence of capacitance the delivery cable This capacitive impedance factor is of negligible consequence as an alternate path when a subject is in the cage It does not affect the stimulus parameters from a perceptual or behavioral standpoint NOTE WELL You must take all of the above variables
41. h automatic pellet replacement feeding monitors and optical lickometers even while running other complex interactive protocols There is also a switch to select a high sensitivity for smaller subjects such as mice and a lower sensitivity for larger subjects such as rats These settings are referenced to our standard cages When using larger subjects and or different environments select the sensitivity that gives the best dynamic measurement range This activity monitor is also available for use on banks of rack mounted home cages where there is no clearance for ceiling mounting the sensors Special sensors with a different lens geometry are used in this system to normalize movements in the front and rear of the cage when it is viewed from the front Variability between sensors is typically less than 5 when factory adjusted using our E61 03 calibrated motor driven target The target is one inch in diameter four inches long and held at a constant temperature of 40 C to 410C 1040F to 105 8 F by feedback control to simulate body heat 34 H25 01 CEILING CHAIN For both rat amp mouse The Ceiling Chain mounts on top of the operant cage the shuttle cages and the hexagon octagon or dodecagon hubs It mav be used for a simple operant response for escape avoidance or positive reinforcement The bead chain climbing response is ideal for escape designs Hanging on the chain to avoid grid shock is useful in fatigue and motor studies W
42. he light source Rotate them to the desired elevation but note that the source and sensor must be at the same angle so the beam is aligned ROTATE FOR ELEVATION ADJUSTMENT SENSOR INSTALLED MOUNTING HOLE 28 H20 95 PHOTOCELL SENSOR BAR This device consists of separate emitter and detector bars There are five beams on one inch centers The detector bar connects to any Switch input on the ECB like any switch tvpe response sensor Beam breaks are reported as standard 28V signals if any one of the 5 beams is broken The LED emitter light source bar connects to a 28 V connector Because this unit is used only to report animal position it operates in a continuous mode for the entire duration of the break No pulse or delayed mode are available as in the H93 03 above NOTE The H20 95 is part of the Rat and Mouse Shuttle Cages and need not be ordered separately H21 03M H21 03R RESPONSE LEVER This manipulandum is designed for small mammals H21 03R is suitable for rats as well as squirrel monkeys and the H21 03M for mice The switch is a high reliability sealed unit The minimum actuating force of the H21 03R is 25 G and the H21 03M is 4 G H21 05R OMNIDIRECTIONAL RESPONSE LEVER This is similar to the H21 03R lever except that it can be actuated by pressing off center in any direction in a full 360 circle The actuation member is a stainless steel tube protruding 75 inch past the front panel Actuation force is compara
43. hen the animal pulls or climbs on the chain the switch closes and reports the response to one of the Switch inputs on the ECB The chain is corrosion and chew proof stainless steel and is long enough to reach the floors of the cages and hubs It may be cut to the desired length H25 06 UNIVERSAL CEILING MOUNT BUMP ROD For both rat amp mouse This unit mounts on the ceiling of the operant cage the shuttle cages and the hexagon octagon or dodecagon hubs in the same manner as the chain It reports a response to one of the Switch inputs on the ECB any time the stainless steel rod is moved off plumb in any direction The rod comes down to within one inch of the floor of the test cages and shuttle cages It may be cut for use in the hubs which are not as tall as the cages ACCESSORY MODULES H21 01 HAND SHAPING SWITCH For both rat amp mouse The hand switch is used for experimenter control and hand shaping It has a standard 6 pin module receptacle in parallel at the end of the cable for connecting any response sensor module in the arena if desired This allows either the experimenter s switch or the animal s response switch to report a closure It connects to one of the Switch inputs on the ECB and has an 8 foot cable so the operator may sit well back from the arena H21 02R H21 02M EXPERIMENTER S MANUAL SWITCH MODULE This switch mounts in the bay tracks of any arena like any S R module but the switch is on the
44. into account when replicating other studies using shockers that may read the shock after the scanner resulting in a very low reading due to the meter s mechanical integrating properties The specifications of the shock pattern waveform sine or square pulse or how the current is read are rarely reported in recent publications If you have read that 0 1 to 0 3 milliamps is sufficient to produce reliable avoidance behavior in shuttle or passive avoidance designs chances are it was measured post scan and or with the animal in the circuit If so the pulse current was actually much higher than the reported value This shocker reads the unpulsed DC current level through a calibrating resistor for precise setting 2 POLE VERSUS SCANNER OPERATION If a two pole cable is used with the Two Pole Scanned switch in the scanned position the shock stimulus output on the two pole cable will have only 2 9 the shock time density it would have with the jumper in the two pole position This is because placing the jumper in the two pole position changes the reset point on the distribution counter from position 9 to position 3 causing the counter to scan only 2 instead of all 8 grids plus the 9 or home position An effective shock will be delivered but does not have as great a time density pattern as with the jumper in the position Note also that the meter again due to its mechanical integrating properties will read even lower in appr
45. ion program and on the Linc front panel the magazine light is shown vellow and the operate command is shown as red This was done in the interest of claritv O FEEDER when looking at the displav Considering that you can combine 2 or even 4 Linc s to serve a single station this is unlikelv to ever be a necessitv Even if vou split Lincs to serve 2 stations each vou will still have 2 feeders per station But vou can still run 2 more in each station by using the 2 Cue control s in each half of the Linc The limit would then depend upon the power supplv in the base and probabilisticallv how often each is operated after all this would be 4 feeders in each of 16 stations or a total of 64 This is generally considering that most conceivable protocols only operate one feeder at a time making 16 the worst case Still this is too many you can operate about 12 feeders at the same time so don t use a yoked protocol for more 4 FROM The diagram above covers the following modules ECB MARKED FEEDER H14 01M amp Pellet Deliverv Trough H14 04M 8 R Liquid Solenoid Valve H14 05M amp Liquid Dipper H14 06R Combination Dipper Pellet Trough H14 10R Grain Feeder FEEDER H14 22M 20 amp R 45 Pellet Feeders All feeders are operated via one of the Feeder connectors on the ECB Magazine light Pass through connections for H14 01M amp R Pelle
46. king operant and other applications The system also features other arenas for both rat and mouse format modules These include a square hub a hexagon hub an octagon hub a dodecagon hub in the rat size only an instrumented running wheel in the rat size only as well as interconnecting runways and goal boxes for both These hold the same S R modules as the respective sized cages above The runways may be used to connect any of the large cage or hub arenas or to build a variety of linear angular and radial arm mazes The runways are built using module plates as the end walls so that they may be slipped into any wall section Runways may be fitted with either one or two guillotine doors to allow the experimenter to control access to and from any runway hub cage wheel or other device Hubs especially the octagon may be used as multi bay operant cages to hold more levers feeders and other S R modules or they may simply act as runway intersections for a nearly infinite variety of mazes CONNECTION and CONTROL THE HABITEST LINC LINC SWITCH INPUTS CUE LIGHTS TONE AUX SWITCH INPUTS CUE LIGHTS TONE AUX NUMBER POWER 1 2 1 1 1 2 1 1 a 5V 3 4 2 2 3 4 2 2 7 28V SPARES FEEDERS H LITE SPARES FEEDERS H LITE 1 2 OP 1 2 1 2 OP 3 4 1 2 ML A 3 4 1 2 ML B 0 7 ONLY et THE H02 08 HABITEST LINC MAY BE RUN AS A SINGLE STATION AS 2 STATIONS OR IT MAY BE GROUPED WITH UP TO 3 OTHER LINCS FOR A VERY COMPLEX STATION
47. lacement of the pellet This method is far superior to the load cell scale and A D converter method commonly employed in many labs today 22 H14 22M 20 H14 22R 45 PELLET FEEDERS 495 dispensing 85mA quiescent The H14 22M 20 uses 20 milligram pellets or tablets and the H14 22R 45 uses 45 milligram pellets or tablets These sizes are appropriate for mice and rats respectively The disk in the bottom of the pellet hopper turns VA turn for each dispense command signal and brings one of 4 holes over the drop tube to drop a pellet into either the Pellet Trough or the Combination Liquid Dipper Pellet Trough The Pellet Feeders require at least a one millisecond pulse at 24 to 30 Volts 28 Volts nominal to deliver a single pellet When used with a Pellet Trough or Combination Liquid Dipper Pellet Trough the Pellet Feeder is operated via the pellet feeder connector coming out of either as is shown on page 19 The Pellet Feeders can be operated continuously in applications that require the delivery of multiple pellets The control command to deliver a single pellet must be a minimum duration of one millisecond A one unit state duration of 20 50 or 100 milliseconds in Graphic State Notation will deliver 1 and only one pellet To determine the signal duration needed to drop additional pellets to be in a continuous burst multiply the number of pellets by 560 milliseconds e g 2 pellets requires 1 12 seconds of operation
48. ned switch located on the rear of the cabinet in the two pole position the unit functions as a two pole shocker with a two pole bipolar current polarity reversal square wave output Since three equal time intervals off constitute a stimulus cycle the repetition rate is 40 Hz for a line frequency of 60 Hz and 33 33 Hz at 50 Hz Placing the switch in the scanned position expands the number of output poles distributing the polarity reversal across an 8 pole output which is repeated every 8 grids at the shock floor of the arena or runway SPECIFICATIONS Power On Off Switch Remote Manual Switch Remote Operate Input Meter Range Switch Manual Set Shock Shock Routing Calibrate Load Max Prog Control V In Maximum Output Current Maximum Output Voltage Output Waveform Regulation Calibrate Load AC Switching On rear panel Remote Up enables external operate Off Center disables remote operate Manual Down operates shocker manually Turns shocker on when external voltage is applied 5 position switch selects meter range Set shock manually while reading the meter Set Test Routes shock to a dummy load for setting or to subject 50K Ohms simulates a very high value of subject resistance for more precise setting and more accurate delivery over resistance changes 5 Volts 0 2 5 volts 0 5 milliamps maximum output 5 milliamps into 75K Ohms 450 volts Square pulse At 500 micr
49. o be controlled by using a guillotine door at one or the other end of a runway RESPONSE SENSING MODULES H10 39R NEST RETREAT BOX The Nest Retreat Box has a floor switch to determine if the animal is in the box A composite floor stands 1 2 off of the bottom and rests on an adjustable force switch Weight sensitivitv is adjustable bv a spring and screw Adjustment need be made if the weight of bedding if available to the animal or the weight of pups in a maternitv protocol exceeds the weight set at the factorv may be mounted in any hub at the end of a runway in the rat shuttle cage or test cage However owing to its large size it cannot be mounted with most other modules in the adjacent bays in either of these cages because unlike the radial array of module bays the hubs the bays in the cages a single plane The back wall and floor assembly is removable simply by opening two catches for access to the animal cleaning tending and monitoring of pups or replacement of nest material 27 H20 93 SINGLE SENSOR The single photocell beam break detector connects to anv switch input on the environment connection boards The board contains all the necessarv circuitrv to power the infrared LED source and photodetector Beam breaks are reported as standard switch closures An onboard jumper be positioned the user to select different modes of operation for each application Vou mav select
50. o initiate state flow if your protocol is designed to auto start in this manner 26 RESPONSE SENSING THE OPENING OF THE COUPLER W LOCKABLE DOOR H10 37R LD COUPLER W LOCKABLE DOOR 295mA This arena and runway coupler has a switch to report the animal s pushing on the door The switch report see previous is activated when the door is in any position other than free hanging straight down The report is made either when locked by the latch and pushed or when it is free and the animal passes through The switch report can be used to activate the latch see page 11 when the animal tries to pass This allows the correct door to be set up so the sound of the latch operation at the beginning of a trial will not cue the correct door The switch can also be used to report attempts at a incorrect or locked door The H10 37R LD Coupler with Lockable Door is an alternative to the guillotine door to control access between arenas with the advantage that it can be locked to prevent passage while at the same time be monitored to record attempts to pass RESPONSE SENSING TURNING IN THE RUNNING WHEEL H10 38R SHOCKABLE RUNNING WHEEL Use H10 37R DP 09W for Extra Drop Pans The running wheel features a shockable grid floor a revolution sensing circuit to count turns and a drag brake on the axle to allow for control of the force necessary to turn the wheel The brake is controllable for or off by a s
51. oamps 2 at 0 to 900K Ohms At 1 milliamp 2 at 0 to 450K Ohms At 2 milliamps 2 at 0 to 225K Ohms 47K Ohms Zero Crossover Scanner Section The grid outputs are semiconductor bipolar MOSFET switches The off grids have zero resistance so the subject receives the same current regardless of the number of grids contacted AC Switching Indicators Dimensions Electrical INSTALLATION Zero Crossover Power On Shock Test Load and Shock Subject 10 W x 7 D x 3 1 4 H 110 120 VAC 50 60 Hz or 220 240 VAC 50 60 Hz specify on order 1 Attach the AC mains power cord to the AC input on the rear of the shocker and plug the cord into an appropriate AC mains outlet 2 Attach the electrodes or shock cable to the 8 Pin connector on the rear of the shocker 3 Turn the AC power switch on the back of the shocker to OPERATION Power On Off Switch On rear panel Remote Manual Switch Up Enables an external signal to operate the shocker Center Disables remote operate Down Operates shocker manually for duration of depression The down position of the switch is spring loaded to return to center when released Remote Operate Input On back panel turns shocker on when external voltage is applied 5 to 30 VDC at 20 milliamps Mode Range Switch 5 position switch selects three ranges in the programmed mode two ranges in the manual mode 15 Manuallv Setting V S Programming the Shock Level
52. ogram being sure the device House Lite or Single Hi Bright Cue is plugged into the connector you select The diagram below shows the connections for all of the visual stimuli controlled by the Triple Cue connectors Next to the diagrammatic representation of the connections on the left there is a column for the 7 Pattern displays the Triple Cues and the Tricolor Cues Note that the decimal stimulus numbers represent the binary coding of the 3 stimulus bits The control line in the spare connector is the same conductor as the one controlling the green stimulus in the Cue connector it is the least significant bit If you use a Spare command for a three stimulus module it will light the green light or the single dot in the 7 pattern display 7 PATTERN TRIPLE FEEDER SPARE TONE DISPLAY CUE HOUSELITE Om OFF O Om STIM1 OO Om STIM 2 Om STIM 3 OO O Om STIM 4 e00 O Om STIM 5 NOTE The feeder mag lites spares tone and OOO STIM6 house lite color codes here do not correspond to colors of the lights on Linc front panel This was done so that thev would not be confused with nearbv lights 11 STIMULUS MODULES AUDITORV STIMULI H12 SERIES The auditorv module group like the visual stimulus module group on the previous page uses either Spare connectors or Cue connectors As with the visual group auditorv stimuli mav be operated bv either tvpe connector Singl
53. on signals or input events generated by the Habitest system are 28 Volt signals The signal is reported via a Switch input jack on the ECB to the Habitest Linc Some of the response sensors detect responses using a simple mechanical switch many years ago most of them did thus the continuing 28V POWER designation on the ECB Others sensors use modern electronic sensors and signal conditioning of varying degrees of complexity RESPONSE INPUT LINC COMMON POWER The same connector jack is used as is used for the Cue and Spare outputs that come out on the bottom 3 pins One side of the switch is connected to the top left 28V pin and the other side of the switch is connected to the top center pin to report to the Linc The top right pin is the RESPONSE SWITCH INPUT other side of the 28 Volt power supply and CONNECTIONS provides the common positive for the electronic response sensors as well as the stimuli Response sensors are generally for the duration that the sensor is being acted upon For example when is in an 21 09 O9R Nose Poke or an animal s head is in a feeder magazine which has been fitted with an H20 93 Single Photocell Sensor and the photo sensors on either of them are set in the Continuous mode When an animal is in a Nest Retreat Box or a lever is being held down the output is also a continuous signal Other response sensing devices generate pulses
54. onversion is known as time integrated serial pulse A D conversion Converting the pulses into units of measure like gram seconds is done automatically by Graphic State Notation It has point and click tables for our platforms and transducers that you select when you create a data analysis element computational element to tell the program what conversion factor to use This is more fully covered in the Graphic State Notation User s Guide Time integral or serial pulse analog measures that are useful in the analysis of behavior include things like 1 Ergometric force applied either to a force transducer like an ergometric activity tremor platform or to a TIME INTEGRATED SIGNAL GRAM NEWTON MILLIVOLT SECONDS EACH PULSE REPRESENTS A NUMBER _ lt GM SECONDS NEWTON SECONDS MILLIVOLT SECONDS ETC GRAPHIC STATE ANALYSIS lt ELEMENT CAN PARSE PULSES INTO A VARIETY OF HISTOGRAMS CONVERTING ANALOG SIGNAL REPRESENTING RESPONSE MAGNITUDE INTO DISCRETE EVENT PULSES AND DISTRIBUTING THEM OVER TIME climbing cage wall 2 Aneroid pressures 3 Force applied to press plate key or lever 4 Moving an accelerometer All can be measured as a series of pulses having an instantaneous rate or frequency conversely period or IET which is proportional to the rolling average of the measure s amplitude For proportional analog measures performed in behavior analysis this is the preferred method because
55. or the shock in any state in the Graphic State Notation program double click on the Prog button in the state graphic and select using the window NOTE Manual Positions Use one of the 2 switch settings below when using the shocker in the manually set mode to deliver the same shock for each presentation Man I Lo Position To set 0 to 0 5 milliamps use the Lo range position and read the 0 1 upper meter scale Man I Hi Position To set 0 to 3 milliamps use the Hi range and read the 0 3 lower meter scale To set values above 3 mA use the LO range and set 0 3 to 0 5 milliamps while reading the 0 1 upper meter scale then set the range to HI before delivery Over ranging the meter to 5 will not damage it Shock Routing Switch Set Test Routes shock to a dummy load resistor for setting shock manually or checking and testing the external program control signals via the optional programmed level control Shock is not connected to subject output cables when the switch is in the set test position The green light indicates that the shocker is being operated with shock being routed to the test calibrate load When you create a protocol in Graphic State you may want to run it in the Pilot mode to check functioning of the program This is a good time to check the shock output levels you programmed in the protocol Just set the switch to the Set Test position for the pilot run and read the meter Subj
56. outside of the arena where it may be pressed by the experimenter It is connected to one of the switch inputs on the connection panel Use this switch when the starting of an experiment must be accomplished as soon as the animal is placed in the arena rather than from the computer and Linc location H29 01 BALANCE ARM FOR ALL TEST CAGES H29 01HP BALANCE ARM MOUNTING PLATE FOR HUBS This device mounts directly on the roof of any H series cage or on octagon or hexagon hubs with the mounting plate It is used to carry electrode leads catheters swivels and commutators The balance weight on the arm may be moved like a counterpoise to balance the lead or electrode weight slightly negative and then locked in place H29 05R H29 05M FAN MODULE The fan module is used to boost air exchange from any runway hub or cage It is generally used for olfactory studies to flush air from the arena in the vicinity of controlled olfactory stimulus delivery devices or to keep free flow olfactory stimuli in multi arena environments from mixing 35 H10 37R GDM H10 37M GDM RUNWAY GUILLOTINE DOORS These manual doors consist of a metal plate with a finger hole at the top They slide into a slot in the bracket that joins the wall to the faceplate at the end of all runways You may use them simply as manual doors to open or block certain segments of complex working arenas by using the finger hole to lift them see below They are also useful when using a go
57. oximate proportion However do not infer that the shock is diminished perceptually by a similar fraction itis not It is the instantaneous current in a pulse that correlates most closely with perceived aversiveness Set Point Current is only an indication of the relative aversiveness of a shock and the resulting behavior when time density inter pulse interval relative to nerve and muscle absolute refractory periods electrode pattern and route of administration variables are constant Administration variables include electrode contact area cutaneous sensitivity at the site s volumetric density of all tissue in the path and the fractional volume of muscular tissue in that path In short it is a chaotic system in which current set points are generally monotonic with respect to aversiveness in commonly used laboratory procedures employing grid floors or attached electrodes and timed durations of administration long enough to insure that several pulses reach the animal A 100 millisecond operate time insures one scan of the grid floor 20 milliseconds assures one alternation of the two pole output at 60 Hz 17 CABLES H13 16 H93 01 25 8 CONDUCTOR RIBBON CABLE ALTERNATE GRIDS 25 FT H93 01 50 8 CONDUCTOR RIBBON CABLE FOR ALTERNATE GRIDS 50 FT FOR SCANNED GRID FLOOR SHOCK TO GRID FLOOR OF CAGES OR H93 30 FOR HUBS 25 50 H93 19 25 2 CONDUCTOR CABLE PIN TO CLIP 25 FT H93
58. pare stimulus output of the Habitest Linc Revolution counting is accomplished by a magnetic reed switch reporting to the Habitest Linc via a standard response Switch input connector on the ECB The printed circuit board wheel carries the shock stimulus from commutators near the hub to the grid bars around the circumference of the wheel The grids are the same as all of the rat grid shock floors in the system Connection is made using a two pole shock cable for either the H13 01 or the H13 16 shockers When the brake control input is operated by a Spare stimulus output from the ECB the force necessary to run the wheel is increased from approximately 7 grams to approximately 30 grams at 30 rpm at the circumference of the wheel This force is not an absolute brake to stop the wheel but represents an effective resistance in the range of sustainable activity that increases the effort required to turn the wheel The resistive drag braking force is generated by feedback from the magnetic interaction of the windings of the motor when it is turning Resistance is proportional to speed reaching about 30 grams at 30 rpm The running wheel may be joined to any runway or the coupler with or without the lockable door The coupler with the lockable door H10 37R LD may be used to control access If you use either of the couplers you can connect a rat test cage directly to the wheel and the two will fit a cubicle Access to the wheel may als
59. racks of the terminator at the same time Protrusions will not clear the top of the doorway on the plate comprising the end of the runway if you try to install them one at a time 10 STIMULUS MODULES VISUAL STIMULI H11 SERIES All visual stimuli in the Habitest system are controlled via a Spare House Lite or a Cue connector The Cue connector has 3 circuits to control up to 3 stimuli The Spare and House Lite connector have a single circuit to control a single stimulus on the same pin as the red stimulus on the Cue connector see below There are 4 Cue circuits and 8 Spare circuits in each Habitest Linc there are 2 and 4 of these respectively available on each of the 2 ECBs for each Linc As discussed earlier these two connector types can control all stimuli not just the visual stimuli see page 7 To control intensity or color see A21 10 LIGHT INTENSITY AND COLOR CONTROLLER on page 37 NOTE of the connectors in the H series environment are six pin connectors and we have wired them to use the same pins for 28V Ground common positive Response Report and the remaining 3 pins Control command You may use a Cue connector to control any of the single stimulus devices and a Spare connector to control a specific one of the 3 cues in a triple cue device To activate a single visual stimulus device select any one of the Spare stimulus buttons in the state graphic in the GSN pr
60. rather than steady signals They include the Ceiling Mount Activity Monitor the Transducer Monitor and the H21 09M O9R Nose Poke or H20 93 Single Photocell Sensor mentioned above when their photo sensor circuits are set in the Pulse mode BUILT IN RESPONSE SENSING POSITION IN SHUTTLE CAGES H10 11M PA MOUSE PASSIVE AVOIDANCE CAGE Door lifting holding up H10 11M SC MOUSE SHUTTLE CAGE Door lifting Door lifting holding up H10 11R PA RAT PASSIVE AVOIDANCE CAGE Door lifting holding up H10 11R SC RAT SHUTTLE CAGE Door lifting 800mA holding up 128mA Animal transit and position are sensed and reported by a photocell system built into the cages You may select automatic position detection and shock routing to simplify programming or you may use the E20 95 Photocell Sensor Bar output and create states in your program to follow the animal and then operate the shock transfer relays from the states you use to determine its location The former automatic is considerably simpler and the connections for this option are made when the cages are shipped A reset switch is provided to clear the memory for the animal following circuit It is mounted on the top of the cage If the cage is not turned off that is unpowered between subjects it will remember where the last subject was If the next subject is placed in the same side no change will be registered t
61. rom the one output of the one shot to the RED YEL and GRN lamps of a triple cue The light varies from black to the selected color or lamp chosen In the case of a spare with a single cue lamp the only position that will work is the YEL position When the last two positions a chosen RED to YEL or RED to GRN a smooth transition from red to either one of the two colors will occur because the zero output of the one shot provides the complementary signal The color shift with complementary signals will be continuous and linear for narrow band sources like LEDs as will the duty cycle controlling brightness of single lamps Since the color shift and brightness shift are used with many types of lamps and LEDs no attempt has been made to create a table in the analog output section of the Graphic State software relating to perceived brightness Stevens power function etc After all this is not psychophysics it is just cueing Rather 0 250 Millivolts from the AD selection table will produce 0 to full duty cycle in a linear transition from 1 10 000 brightness at OMV to full brightness at about 249 MV It is used in the 5 Bay test cage as a discriminanda for slight changes in brightness or color Here five brightness controllers and five tricolor nose pokes are used for the subject to select oddity as an attention task varies stimulus properties Another application is the control of brightness of the start chamber of the shuttle cage in the passi
62. ronment Connection Boards ECBs via a feed through that is under the gray hinged panel on the rear of the cubicle Route the ECB cable through the slot behind the ECB and connect it to the bottom of the ECB The cubicle can accommodate two ECBs so that a single Linc may be fully dedicated to a single arena in the cubicle using its full complement of 8 response inputs four feeders and all of the rest of the stimulus outputs To mount an ECB just remove 4 thumbscrews from the mounting posts in the left or right position Place the board over the posts and replace the screws The feet installed on the board for tabletop use will not be in the way An exhaust fan powered by 28Volts is built into the cubicle It is wired to a standard module connector mounted just below the fan The fan may be run continuously from one of the Constant 28V connectors on an ECB or be run under program control from a Spare or other stimulus control outputs Cables and wires to devices not provided for on the ECB may be routed into the cubicle via the opening below the exhaust fan This is for connections like the analog lead from the PROG stimulus output on the back of the Linc shock cables or electrode connections and for power cables for special devices such as the Transducer Monitor Boss e sheen 725 e 4 et INLINE S R DEVICES like the Noise Generator Cue Color Control Tone Generator and others are supplied with Velcro
63. stimulus Cue from SINGLE GAS IN the ECB and each solenoid is marked with a color dot corresponding to the color that operates that valve There are 3 tube connection inputs and a common output so that a single gas or a mix of 2 or 3 gasses be routed to 1 location The outputs may be connected to the H15 01M or R above to any feeder magazine or to the H21 09M or R Nose Poke modules to supply gases as discriminative stimuli The inputs and outputs can be reversed so that a single supply gas may be selectively delivered to 1 2 or 3 locations Fittings are available for user installation contact us for details MIX OF GASSES OUT TO A SINGLE LOCATION H15 20 INLINE OLFACTANT EVAPORATION CHAMBERS These chambers have an inlet and outlet barb so that clear air or other gas may be passed through them The volume is sufficient to minimize flow through dilution gradients for typical cueing presentations small amount of liquid or a swab of cotton with the proper amount of olfactant is placed in the chamber The olfactant evaporates into the air in the chamber When the solenoid valve is opened air or other gas 24 bearing the solute olfactorv stimulus is delivered With small percentage of the total volume delivered the concentration gradient over the flow interval is minimized A check valve is supplied with each chamber to be used to prevent back flow cross contamination of the olfactants in each chamb
64. t and communicate with the computer STIMULI Stimuli are physical properties of the environment that are above the subject s threshold of sensation Controlled stimuli are those that are turned on and off by the program via the Habitest Linc cables and ECB using the industry standard 28Volt control signals SWITCH The name for the input on the Environment Connection Board ECB to which response sensing modules are connected This a carryover from early days when all response sensors were simple switches See also EVENTS THE HABITEST SYSTEM HABITEST is a modular animal behavior test system The test cages and other working arenas are constructed using a system of open bays with slotted tracks as wall sections Each section holds slip in Stimulus Response modules and blank panels to close each wall section The system includes four modular test cages There are two large ones three bays facing three bays and one bay facing five bays for rats pigeons small primates and other similar sized animals and two small ones for mice gerbils etc There is a complement of narrow S R modules for the small cages and wider modules for the larger cages these are for feeding drinking operant and other test procedures There are also four modular shuttle cages two for rats one has a blacked out compartment on the left for passive avoidance and the other two for mice These are used for avoidance place preference feeding drin
65. t Delivery Trough and H14 06R Combination Dipper Pellet Trough When using a pellet feeder with a pellet trough or a combination dipper pellet trough the pellet feeder is operated via the connector coming from the trough The trough is connected to one of the Feeder connectors on the ECB The wiring from the board powers the magazine light in the trough The rest of the wires continue along to the feeder carrying the operate commana line and the power lines to the feeder via the daisy chain arrangement See next page for mounting 19 H14 03M H14 03R LIQUID DELIVERV RESERVOIR This is the same deliverv reservoir used in the H14 04M and R USED AS LITE below It is fitted with needle tubing on the rear to receive a ON THIS FEEDER catheter type tube Fluid is delivered by an infusion pump rather than by the gravity feed reservoir with a solenoid valve the model below It is used for greater precision or when only small volumes of fluid are available owing to their great cost No magazine light is available because this is not an active feeder module but you may use an H11 Series cue above the reservoir in place of a magazine light if necessary H14 04M H14 04R LIQUID SOLENOID VALVE 400mA ja The Liquid Solenoid Valve should be used with water or solutions OPERATE in which all ingredients will evaporate If used with solutions that leave a residue such as salt or su
66. tch reports the response Generallv the grid floor tos is one pole all of the grids shorted together by the special grid floor 7 connector supplied with the unit and another isolated object is the ka other pole This device mav be used to sense animal contact with novel stimulus devices mounted on a module Vou can make vour own using blank plastic module plates 90 00 2 0 4 0 You may also hang things from the cage ceiling or sit them on an isolated pad or base on the floor 32 24 61 H24 61 MC INFRARED ACTIVITY MONITOR For both rat amp mouse H24 61 MC SUSPENDED CEILING KIT FOR MOUSE CAGE This detector mounts on the top of standard test cages shuttle cages and the hubs It mounts in the standard ceiling hole array on the tops of these arenas An optional suspended ceiling kit is available H24 61MC for the mouse cage to lower the sensor for increased sensitivity The kit consists of a false ceiling plate to hold the H24 61 and 4 blank module plates with pins to support it The plates like any module may be mounted at various elevations The wide field of view will detect animals while ACTIVITY MONITOR MOUNTS ANGLE OF VIEW IS 90 rearing or leaning wall It can see both rats THE ON ARENA CEILING and mice in this posture and senses movement CVCOULBOURN 8 at anv elevation in the cage For flexibilitv in defining the response for program control
67. the spatial topographic and temporal nature of overt behavioral responses are not suited to periodic instantaneous sample by multi bit A D converters If an event is to be proportional it must be designated as such in the event name list and bear the prefix To record these types of signals and present your data in units of measure corresponding to the transducer double click on the event name box in the top create a protocol window Then scale your graphs by selecting the proportional option for that event this will also append the P A pop up window will appear for you to specify the units of measure that the event represents Here you select units such as gram seconds or Newton seconds for force PSI or Pascal seconds for pressure g seconds for acceleration or other amplitude time integral units This is very simple to do when you are using Coulbourn Instruments or similar transducers and our E24 72 Transducer Monitor In the event type box click on the button labeled proportional to select the 40 unit of measure This will change the default unit from a discrete named event to a proportional It will also bring up a window with a chart of our transducers bv model number The chart for each has one or more units of measure for tvpe of transducer measures along with a box to select the setting you have already made or will make on the switches on the transducer monitor Here vou mav select th
68. the industry standard 28 Volt response sensor signals from the Habitest or other environment s levers photocells etc All of the words response response input switch input switch input event or event input are used interchangeably in this manual They refer to the inputs on the ECB to which the Habitest response sensing modules are connected on the environment connection board and to the corresponding inputs and indicator lights on the Habitest Linc INPUTS See EVENTS MODULES STIMULUS amp RESPONSE The panels that hold the stimulus producing and response sensing devices for the Habitest system The panels slide into the tracks of the arenas to form the walls LINC The Habitest Linc is the interface module that connects the environment to the computer RESPONSE See also EVENTS Any behavior of the animal that is above the threshold of one of the system s input sensors and is therefore measured a reported behavioral event This may include responses the animal makes on an operandum or a behavior recorded by an incidental response sensor like a ceiling mounted activity monitor an ergometric force platform STATION A station is the total set of environmental hardware dedicated to running one animal It includes the arena with stimulus and response modules in which each animal is run and the interface hardware necessary to present the stimuli and the input sensors to register response events for one subjec
69. to the visual MOUNT WITH APERATURE DOWN stimulus displavs on page 11 for patterns 29 H23 17M H23 17R RETRACTABLE LEVER moving quiescent ITCH l quiescent fu lt These levers incorporate the same paddle switch 0 iE force and throw characteristics as their fixed counterparts H21 03M H21 03R see above They rE OTOH are fitted with high speed stepping motors that extend ioni SPARE W and withdraw the lever Retraction is activated bv a 28 VDC signal via a Spare stimulus output gpm H24 01M H24 01R OPTICAL LICKOMETER use also as free access water bottle U S Patent No 4373471 PHOTOBEAM DRINK TUBE PHOTOBEAM DETECTOR 4 EMMITER The optical lickometer is used to measure licking drinking from a standard drinking tube It requires an H20 93 Photocell Buffer see pg 28 for mounting The photo detectors LED source and sensor are mounted on either side of the module The light beam is piped via glass rods to the tip of the drink tube across a gap at the end of the tube The animal s tongue breaks the beam on each The H20 93 must be set in the pulsed mode for use with the lickometer so that the capacitive coupling can pass relative changes in optical densitv to assure that licks are recorded ANIMAL S TONGUE BREAKS PHOTOBEAM even if there is a meniscus from the lips to the tube Twentv TO REPORT A LICK TO THE INTERFACE licks per second
70. tubes replace the standard gravitv flow tube and bottle For precision flow control and conditional availabilitv to the animal fluid is pumped on demand from an infusion pump The TB 01 model has a single reduced diameter lumen The TB 03 model has three reduced lumens a single standard outer diameter tube so that three different fluids mav be delivered via the same tube The tip of the controlled flow tubes are as close in shape as possible to the standard tube both for behavioral and response measurement reasons They differ from the free flow type tube in that they have needle tubing inside the standard drinking tube with avery small inside diameter to prevent air replacement flow The result is that when fluid is not pumped no fluid may be taken The outer dimensions are the same however to insure that the lick topography and the counting of licks are comparable to the standard tube DO NOT MOVE FACTORY ADJUSTED COLLAR CONNECT TO PUMP MINIMUM DISPLACEMENT WATER TUBE OPTICAL LICKOMETER APPLICATION NOTES The drink tubes are mounted in a Delrin block and electrically isolated from the rest of the module Conflict Lick Suppression Vogel Procedure Conditioned Suppression or other protocols involving shock via the drink tube are easy to accomplish Aversive shock may be delivered through the drink tube without electrifying the module or cage walls To do this connect one pole of a shocker to the drinking tube with an
71. umbscrews The sensor window is placed with the lens facing down into the cage over the large hole on the roof of the rat modular cages the lids of the hubs or the H24 61MC suspended ceiling for the mouse cage The thumbscrews are inserted through the small holes near the large lens hole and then tightened to secure the unit Dimensions 10 16 cm x 15 24 cm x 3 81 cm 4 inches x 6 inches x 1 5 inches Monitoring the change in position of its infrared body heat image is accomplished by using dual element 13 nanometer infrared heat sensor in conjunction with a multiple lens array The array looks at the subject and focuses the multiple images one for each lens in the array on the sensor s active heat sensitive area Assume that the red spot in the diagram below represents an animal in the center of a cage or other working arena Each lens element sees the spot from a slightly different perspective The sensor has two far infrared photosensor elements connected together as an AC coupled differential amplifier so that it has maximum sensitivity to differences in the changes in light heat energy falling on the two elements 33 SI SUBJECT LENS ARRAV DETECTOR SENSOR ELEMENTS SUBJECT IMAGES OVER ELEMENTS With the arrav of images from each lens focused on the sensor anv movement results in a change in infrared energy falling on each element because each image partially or totally falls off of one or comes onto th
72. ve avoidance task Here the high bright cue lamp is driven by the BLK to YEL selection of the controller to vary the brightness of the start side These two applications illustrate the use of both LED and incandescent lamps as well as the use of modules fitted with both triple and single cue lamps 37 24 72 RESISTIVE BRIDGE TRANSDUCER MONITOR This device allows any resistive bridge transducer to be used in the Habitest system It has two standard response switch outputs one for threshold detection and the other for a serial pulse proportional output The threshold output is on when the transducer is above a user selected setpoint This function is comparable to a comparator or signal level trigger It is used for setting force pressure or other threshold requirements to produce an event type input The serial pulse proportional output is a serial pulse analog to digital converter employing time period integration This function is comparable to a cumulating resetting integrator It is used to record ergometric aspect behaviors in a time amplitude integrated mode It may be used with any bridge transducer but is most commonly used with the Startle Tremor platforms for ergometric activity or with a stand mounted transducer The proportional output produces a pulse stream the frequency of which is proportional to the magnitude of the signal from the transducer more force more pressure etc the faster the pulses typically from a few
73. with the levers on either side Each access port is the same size as in the H14 01R and H14 05R The ports are separated with the liquid to the left The 2 magazine lights are operable independentiv When using an H14 01R Pellet Delivery Trough with an H14 22R 45 Pellet Feeder the feeder must be centered on the module because ie RIGHTHAND HOLES CENTER THE DELIVERV TUBE A LEFTHAND HOLES OFFSET THE DELIVERY TUBE want to use the H14 06R Combination Dipper Pellet Trough with the feeder vou must offset the feeder mechanism on the feeder FEEDER panel so that the drop tube mav be MODULE inserted in the pellet chute when install the feeder module above it 1 UNIT There are two screws in the center BLANK of the panel and three tapped 4 threaded holes in the bracket which are used to secure the mechanism to the panel Remove PELLET the screws and screw them into the ROUGH proper two holes shown at the top COMBINATION right for the type trough you are DIPPER using PELLET TROIIGH Also note that the H14 01R Pellet Delivery Trough is a 2 unit high 1 UNIT module and requires a blank filler BLANK module above to space the drop a TILLER tube properly The blank at the bottom brings the magazine opening up to standard height DIPPER CUPS H14 06 CP series The dipper arm used in the H14 05M 05 and H14 06R dippers is finished with a 01 cc volume cup machined directly into the end of th

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