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OPERATING INSTRUCTIONS AND SYSTEM DESCRIPTION FOR
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1. DB INS truments for the Life Sciences OPERATING INSTRUCTIONS AND SYSTEM DESCRIPTION FOR THE 9 CHANNEL EXTRACELLULAR AMPLIFIER SYSTEM FOR 2 TETRODES VERSION 1 1 npi 2010 npi electronic GmbH Hauptstrasse 96 D 71732 Tamm Germany Phone 49 0 7141 9730230 Fax 49 0 7141 9730240 support npielectronic com http www npielectronic com Tetrode Amplifier User Manual Table of Contents ke DALCLY RE SULAUOGIAS aequus ua ai i a Deu ik i a i a i 3 ZEXT STD2 NMI Ch PAGE utate as aa a aa a S nus 4 al SYSTEM DES CII DUOM ai sea a a o A a a a a o a a Ia d 4 2 2 ADescripiomror LMS Front Pane Luo de ai em ai i i aa a OE Cut rue E 4 2 9 JDescripHoIborthe Reir Panel ai in iua etd no ctionis desiit cosi a a S 6 Da ACA SIAC E dia iii ai i i i ii a a i i i i T 7 S SC NIC AAU e k A W RAE oc RR e 7 version 1 1 page 2 Tetrode Amplifier User Manual 1 Safety Regulations VERY IMPORTANT Instruments and components supplied by npi electronic are NOT intended for clinical use or medical purposes e g for diagnosis or treatment of humans or for any other life supporting system npi electronic disclaims any warranties for such purpose Eguipment supplied by npi electronic must be operated only by selected trained and adeguately instructed personnel For details please consult the GENERAL TERMS OF DELIVERY AND CONDITIONS OF BUSINESS of npi electronic D 71732 Tamm Germany 1 2 3 4 5 GENERAL This system is d
2. 500 1000 and corner frequencies of high pass 0 1 0 3 1 Hz and low pass 500 2k 8k Hz filter can be set internally at the PCB by DIL switches and a jumper see also Table 1 and Table 2 for each channel separately version 1 1 page 4 Tetrode Amplifier User Manual DC gain 200 LP 8 kHz DC gain 500 LP 8 kHz DC gain 1000 LP 8 kHz DC gain 200 LP 2 kHz DC gain 500 LP 2 kHz DC gain 1000 LP 2 kHz DC gain 200 LP 500 Hz DC gain 500 LP 500 Hz DC gain 1000 LP 500 Hz Table 1 DC recordings Jumper Si S2 S3 S4 S5 S6 Setting HEN 4 pa Hz gain 200 LP 8 kHz Hz gain 500 LP 8 kHz Hz gain 1000 LP 8 kHz Hz gain 200 LP 2 kHz Hz gain 500 LP 2 kHz Hz gain 1000 LP 2 kHz Hz gain 200 LP 500 Hz Hz gain 500 LP 500 Hz Hz gain 1000 LP 500 Hz HP 0 3 Hz gain 200 LP 8 kHz HP 0 3 Hz gain 500 LP 8 kHz HP 0 3 Hz gain 1000 LP 8 kHz HP 0 3 Hz gain 200 LP 2 kHz HP 0 3 Hz gain 500 LP 2 kHz HP 0 3 Hz gain 1000 LP 2 kHz Hz gain 200 LP 8 kHz Hz gain 500 LP 8 kHz Hz gain 1000 LP 8 kHz Hz gain 200 LP 2 kHz Hz gain 500 LP 2 kHz Hz gain 1000 LP 2 kHz Hz gain 200 LP 500 Hz Hz gain 500 LP 500 Hz Hz gain 1000 LP 500 Hz Table 2 AC recordings version 1 1 page 5 Tetrode Amplifier User Manual Gain amplification factor HP corner frequency high pass filter LP corner frequency low pass filter OE jolojole Jumper O10 ma ch o 960 OOo 0 O O Cou c
3. oo ooon 56 m 5 DIL switch 09 oo B00 Cm aos 66606 o 68000 ce c 000 Q o0 E oo 29 2 t Q O O O OGOO OO S660 666000520 009062 666060 See as Figure 2 PCB of one amplifier location of DIL switch and jumper 2 3 Description of the Rear Panel 2 3 X ce 2 EJ O Figure 3 rear panel view of EXT T2 1 GROUND This connector is linked to the internal system ground which has no connection to the 19 cabinet CHASSIS to avoid ground loops 2 CHASSIS This connector is not linked to system ground and can be used for grounding the CHASSIS independently from system ground 3 IN OUT SIGNALS TETRODE B Not connected 4 IN OUT SIGNALS TETRODE A Not connected 5 POWER SUPPLY connector Connector for the wall POWER SUPPLY 12V 18V AC IA min 6 POWER ON switch Switch to turn POWER ON upper position version 1 1 page 6 Tetrode Amplifier User Manual 3 Headstage D 71732 Tamm IWO 3 act onic Hai es ese a tit AAR r A m AMPLIFIER HEAL Figure 4 one headstage of the EXT T2 Headstage Elements Pg Connector for the tetrode see also Figure 5 REF Not connected GND Ground connector Ground connector GND Channel 1 connector CHO Channel 3 connector CH2 Channel 4 connector CH3 Channel 2 connector CH1 Un amp WN m Figure 5 tetrode connector 4 Technical Data Input resistance gt 107 Q range 1 V Output gain x200 x500
4. x1k selectable by DIL switch Output range 12 V into I KQ 1 V into 50 Q load Low pass filter 500 2k 8k Hz selectable by DIL switch High pass filter DC 0 1 0 3 1 selectable by DIL switch Power requirements 12 18 V AC 1 A min wall power supply Dimensions 19 rackmount cabinet 19 483 mm 10 250 mm 1 75 44 mm Headstage Size 70 x 26 x 26 mm Holding Bar length 150 mm diameter 8 mm version 1 1 page 7
5. esigned for use in scientific laboratories and must be operated by trained staff only General safety regulations for operating electrical devices should be followed AC MAINS CONNECTION While working with the npi systems always adhere to the appropriate safety measures for handling electronic devices Before using any device please read manuals and instructions carefully The device is to be operated only at 115 230 Volt 60 50 Hz AC Please check for appropriate line voltage before connecting any system to mains Always use a three wire line cord and a mains power plug with a protection contact connected to ground protective earth Before opening the cabinet unplug the instrument Unplug the instrument when replacing the fuse or changing line voltage Replace fuse only with an appropriate specified type STATIC ELECTRICITY Electronic equipment is sensitive to static discharges Some devices such as sensor inputs are equipped with very sensitive FET amplifiers which can be damaged by electrostatic charge and must therefore be handled with care Electrostatic discharge can be avoided by touching a grounded metal surface when changing or adjusting sensors Always turn power off when adding or removing modules connecting or disconnecting sensors headstages or other components from the instrument or 19 cabinet TEMPERATURE DRIFT WARM UP TIME All analog electronic systems are sensitive to temperature changes Therefore all electronic in
6. struments containing analog circuits should be used only in a warmed up condition 1 e after internal temperature has reached steady state values In most cases a warm up period of 20 30 minutes is sufficient HANDLING Please protect the device from moisture heat radiation and corrosive chemicals version 1 1 page 3 Tetrode Amplifier User Manual 2 EXT T2 Amplifier Filter 2 1 System Description The EXT T2 module is an amplifier filter with headstages for two tetrodes A and B The signals recorded from every single tetrode attached to the headstage are amplified and filtered and linked to the BNC connectors OUTPUT 0 to OUTPUT 3 for every tetrode Every OUTPUT is available at two BNC connectors The GROUND plug provides system ground Measurements are done 1s single ended configuration against GND 2 2 Description of the Front Panel Figure 1 front panel view of EXT T2 1 HEADSTAGE connector Connector for the HEADSTAGE of TETRODE A 2 OUTPUT 0 OUTPUT 3 connectors BNC connectors providing the amplified and filtered signals of tetrode A 3 GROUND connector Banana jack providing system ground 4 POWER ON LED LED indicating that POWER is switched ON 5 HEADSTAGE connector Connector for the HEADSTAGE of TETRODE B 6 OUTPUT 0 OUTPUT 3 connectors BNC connectors providing the amplified and filtered signals of tetrode A Every channel has a single PCB with amplifier and filters Amplification factors 200
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