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1. Figure 2 13 Final Assembly of the Microlab 300 Series Pipettor Note With all components installed the device becomes slightly top heavy Use caution when operating and avoid bumping and pushing the unit to prevent knocking it over Microlab 300 User Manual 27 CHAPTER 2 INSTRUMENT SETUP 2 9 Applying Power to the Instrument The Controller is powered via the connection to the Syringe Drive Unit Step 1 To power up the instrument plug the country specific power cord into the power supply Step 2 Make sure the Syringe Drive Unit Power switch is set to the O off position Then plug the power supply into the VDC receptacle on the Syringe Drive Unit and plug the power cord into a wall outlet as shown in Figure 2 14 Step 3 Flip the Power switch on the Syringe Drive Unit upwards to the on position It takes approximately one minute for the Controller to boot up completely Power Supply Power Cord p n 51092 01 User Defined a ll Tir Figure 2 14 Power Cord and Power Supply Installation 2 10 Priming the Instrument This section will describe how to properly prime the Microlab 300 before running a method Step 1 Select the Prime button on the Controller Main Menu screen Step 2 Select the appropriate number of cycles and pr
2. Syringe Drive Unit Figure 2 4 Assembly of Controller and Syringe Drive Unit Step 1 Place the Syringe Drive Unit in the selected location Step 2 Place the Controller on top of the Syringe Drive Unit as shown in Figure 2 4 Microlab 300 User Manual 21 CHAPTER 2 INSTRUMENT SETUP A Important The controller should be charged for 12 hours prior to use see Section 2 4 Step 3 Next plug one end of the grey Controller cable into the Controller receptacle on the back of the Controller and the other end of the cable into the Controller receptacle on the back of the Syringe Drive Unit Step 4 Finally insert the SD card label side up into the left side of the Controller The Controller is now properly installed on the unit As shown below the Controller can tilt at different angles for user comfort and preference in addition this Controller may also be hung on a wall r Front Screen IER a gt oan OR Figure 2 5 Controller 2 6 Installing the Cable Management System The Cable Management System is designed to hold the Disposable Tip Hand Probe Included with the Cable Management System is a Cable Manager that eliminates unnecessary cord clutter on the workstation The unit comes with three pieces that are easily assembled the Accessory Holder the Cable Manager and the Probe Holder as shown in Figure 2 6 22
3. Microlab 300 User Manual 77 CHAPTER 10 ICON INDEX 78 Liquid Classes Small Icon Buttons Import Liquid Classes Press to import Liquid Classes from the SD card onto the instrument Export Liquid Classes Press to export Liquid Classes from the instrument onto the SD card Delete Press to delete a Liquid Class User Icon Buttons Edit User Press to edit an existing user Change Key Press to reset a user key CHAPTER 10 ICON INDEX History Press to view the edit history of a user account Microlab 300 User Manual 79 CHAPTER 11 CARE AND MAINTENANCE CHAPTER 11 Care and Maintenance This chapter contains instructions for routine maintenance procedures for the Microlab 300 which includes the following topics 11 1 Daily Maintenance 11 2 Weekly Maintenance 11 3 Yearly Maintenance 11 4 Instrument Calibration 11 5 Cleaning the Microlab 300 11 6 Storing the Microlab 300 11 7 Replacement Component Installation 80 CHAPTER 11 CARE AND MAINTENANCE 11 1 Daily Maintenance It is recommended to prime the Microlab 300 Pipettor daily to ensure the accuracy and precision of the instrument The recommended system fluid is deionized water Over time solutions like water will degas which creates air bubbles in the system The air in the system may lead to inaccuracies during pipetting applications 11 2 Weekly Maintenance System fluid pa
4. eC 94 Problem Persistent air or leaks in the fluid path CHAPTER 12 TROUBLESHOOTING Possible Causes Loose worn or incorrect tubing fittings Fill tubing is not fully submerged in the reservoir Dispense speed is too slow to dislodge the bubbles from the fluid path Damaged or worn syringe or plunger tip Corrective Action Hand tighten the fittings or replace old tubing with new tubing Make sure the fill tube is fully submerged in the reservoir System fluid leaks from syringes Instrument gives inaccurate or imprecise results Instrument does not fill or dispense Unit is overheated Damaged or worn syringe or plunger tip Controller cable is connected into the Accessory port and not the Controller port Air exists in the fluid path Liquid Classes are not set correctly Inadequate ventilation room temperature too high or duty cycle too high Microlab 300 User Manual Check to see that fill tubing is totally submerged in reservoir Check to see that tubing connections are tight replace if necessary gt Check to see that the syringes are installed correctly and that they are not leaking gt Replace worn or leaking valves or tubing Increase dispense speed to dislodge air bubbles from the system Reset Liquid Classes to the application solution The unit is overheated Power it off and allow it to cool down Resume us
5. Linked Method Editor NI E Aliquot Reverse Pipette Dilution rum into Test tuDe a Loop 1 Liquid Class Aqueous300Default Sample Volume 1000 Tip Type Standard Tip Size 1 ml Pre Wet Mode Off Mix Mode Off A Method Selector lil Forward Edit Delete The second screen is the Linked Method Run screen This screen displays the different applications within the Linked Method that are run throughout the method 42 CHAPTER 5 CONFIGURATION MENU Linked Method gt HD YO DV IT de 4 a Aliquot Reverse Pipette Serial Dispense Information dilute solution to 0 033333 Loop 1 Liquid Class Aqueous300Default Diluent Volume 750 Tip Type Standard Sample Volume 250 Pre wet Mode Off Tip Size 1 mL Mix Mode Off Air Gap Trigger Off d Method Selector 5 1 7 Numeric Keypad This screen appears when changing a numeric value The screen initially displays the current parameter value Pressing a keypad button automatically clears the Current value Enter Sample Volume Value indicator 1 000 0 25 0 1 000 0 Range Indicator Cancel Keypad Buttons Microlab 300 User Manual 43 CHAPTER 5 CONFIGURATION MENU Value Indicator This indicator displays the current value that will be stored when the Enter button is pressed Keypad Buttons These buttons are used to add and clear numbers from the Value Indicator Range Indicator For most parameters only specific values are acceptable The
6. CHAPTER 2 INSTRUMENT SETUP Step 1 Determine on which side of the pump to install the Cable Management System Remove the two plastic screws and carefully install the Accessory Holder with the metal screws and washers as shown in Figure 2 7 Hand tighten each screw until snug Step 2 Loosen the metal thumbscrew on the end of the Accessory Holder Then thread the metal rod through the hole and tighten the thumbscrew as shown in Figure 2 7 Step 3 Attach the Probe Holder by loosening the thumbscrew Position onto the metal rod and tighten as shown in Figure 2 8 Step 4 Attach the Cable Manager onto the metal rod as shown in Figure 2 9 Cable Manager Accessory Holder Figure 2 6 Cable Management System Figure 2 7 Assembly of the Accessory Holder Microlab 300 User Manual 23 CHAPTER 2 INSTRUMENT SETUP oe Sn VL Figure 2 8 Assembly of the Probe Holder Figure 2 9 Assembly of the Cable Manager The Cable Management System is now properly installed on the instrument 2 7 Installing the Disposable Tip Hand Probe 24 Step 1 Plug the probe cable into one of the two probe receptacles on the rear of the Syringe Drive Unit Step 2 Thread the tub
7. Learn more about the LyncStore at www hamiltoncompany com 5 7 User Accounts User accounts may be created to add a level of security to the Microlab 300 system The Users screen gives the system s administrator the ability to add users edit existing users and export and import users via the system s SD card To enter this screen User accounts must be enabled under Security settings and a user with access to modify accounts must be logged into the system For each account that is created a role or multiple roles is assigned to indicate the level of security for each user Roles include Operator Configure Hardware Manage Users Program Wizards and Linked Methods 54 CHAPTER 5 CONFIGURATION MENU 5 1 Add User The Administrator or a user who has the ability to create and edit user accounts may add new users to the system To add a new user simply fill in the User ID First Name Last Name and set the user role Once all the settings are input the Users button on the bottom left of the screen will be enabled Press this button to exit the screen and save the new account When you exit the screen the system will automatically generate a user key for the new account Record the User ID and the User Key for future reference It is suggested to modify the user key for the new account upon initial log in to the instrument 5 7 2 Edit User This screen is used to modify the settings of an existing user account A user account con
8. Step 1 Before installing the tubing pre wet both tubing fittings with deionized water Step 2 Make sure the tubing is free of crimps or blockages Crimping or blocking within the tubing may cause leaks or air bubbles If the tubing is damaged do not use it and replace with new tubing Step 3 Insert the fill tubing fitting in the left port on the valve and turn clockwise to tighten as shown in Figure 2 12 Figure 2 12 Assembly of Fill and Probe Tubing Step 4 Place the blunt end of the fill tubing in an appropriate reservoir filled with deionized water Cover the reservoir with an appropriate cap or Parafilm to prevent contamination of system fluid Note The fill tubing can be shortened by cutting it to meet specific customer needs Step 5 Insert the probe tubing in the right port on the valve and turn clockwise to tighten 26 CHAPTER 2 INSTRUMENT SETUP WARNING Keep fingers away from syringe drive and from between syringe plunger and bottom of syringe barrel to prevent pinching of fingers Congratulations you have successfully completed the hardware installation of the Microlab 300 Series Pipettor pannen OGD AIA Ac SEE 000000000 LEN
9. User Manual a Ee h ge D e g ipDettor HAMILTON Warranty Information Hamilton Company warrants this equipment to be free of defects in material and workmanship for 12 months from the date of receipt The warranty does not cover normal wear and tear of the syringes valves or equipment The warranty extends to the buyer of record on the original purchase order to Hamilton Company Hamilton Company or an authorized Hamilton representative agrees to repair or replace at its option and free of charge to the buyer at a normal place of business or at a Hamilton repair facility any part or parts that under proper and normal use appears to be defective during the warranty period Abuse unauthorized replacement of parts modifications or adjustments made by other than Hamilton Company or its authorized representative voids this warranty This warranty grants you specific rights No other warranties expressed or implied including implications of warranties of merchantability and fitness for a particular product are made Hamilton Company s product liability shall be limited to repair replacement or price refund of any defective product Hamilton Company endeavors to provide prompt and satisfactory service 1 All Hamilton Company valves are warranted to be free of defects in material and workmanship at the time of delivery 2 Hamilton Company reserves the right to refuse to accept the return of any instrument or valve th
10. 61500 04 The printer upgrade kit contains an HP printer and an additional accessories box Components required for connecting the printer to the Microlab 300 include black and color inkjet cartridges a USB cable a power supply and a country specific power cord All other components in the HP printer box are NOT required to connect to the Microlab 300 Step 1 Power off the Microlab 300 Step 2 Insert of end of the USB cable into the USB port on the back of the Controller as shown in Figure 2 15 Step 3 Insert the other end of the USB cable into the printer connection port Step 4 Connect the power supply to the printer Microlab 300 User Manual 29 CHAPTER 2 INSTRUMENT SETUP Step 5 Connect the power cord to the power supply Step 6 Plug the power cord into a wall outlet The Microlab 300 is now connected to the printer Step 7 Power the Microlab 300 on and then power the printer on Figure 2 15 Installation of the Printer 30 CHAPTER 3 INTRODUCING THE MICROLAB 300 SOFTWARE CHAPTER 3 Introducing tne Microlab 300 Software The Microlab 300 Series Pipettor comes with user friendly software allowing users to easily create save and execute complete pipetting protocols This chapter provides an overview of the user interface such that a user can navigate the screens 3 1 User Interface Flow Chart Microlab 300 User Manual 31 CHAPTER 3 INTRODU
11. Avoid touching tips discarded into the laboratory supplied waste bin Any surfaces on which liquid is spilled must be decontaminated CHAPTER 5 CONFIGURATION MENU CHAPTER 5 Configuration Menu This chapter describes how to configure the instrument including the following topics 5 1 Overview of Screen Terminology 5 2 Liquid Classes 5 3 Hardware Configuration 5 4 Software Configuration 5 5 Security Settings 5 6 Log Settings 5 User Accounts Microlab 300 User Manual 37 CHAPTER 5 CONFIGURATION MENU 5 1 Overview of Screen Terminology This section describes the features of the eight major screen types used throughout the user interface 5 1 1 Icon Screens 5 1 2 Run Screens 5 1 3 Data Entry Screens 5 1 4 Pop up Control 5 1 5 Pick List 5 1 6 Linked Method Screens 5 1 7 Numeric Keypad 5 1 8 Alphanumeric Keypad 5 1 1 Icon Screens The icon screens allow basic navigation through the user interface General screens like the Main Menu or Wizard menu commonly use this screen to display all possible navigation options 38 aia q TMICrOLAB 7 WE T Icon Buttons Prime Wizards ga em WW gy Configuration Utilities CHAPTER 5 CONFIGURATION MENU Icon Buttons These buttons allow major navigation among functions within the user interface Back Button Pressing this button in the lower left of the screen displays the previous screen Small Icon Buttons These buttons at the bottom of
12. DEE EE EE NR en EE EE EEE NN i EE Er er A A a aaa i A EE SE EEE ee EE RENE A EEN GE EE TE EE EE IE E E NEE Ge SE 5 ED ne EE EN Microlab 300 User Manual 105 APPENDICES A 106 Chemical PTFE Borosilicate Glass CTFE Kel F DEE EE Ge DEET EE i An GE de EE eh Se EE EE E a e g EE EE eines rn a EE E EEN DEE er TE ET EE SA EEN Babette EE EE a EE EE EE S dee GEET dE Se SE GEET EE E SEET EE EE S SE SET EE i Ee EEN EE GEES SEENEN E E SC picas EE EE GEET EE BEE e GEET EE oo A a dE laa EN ME ea EEE er A EE 5 es EE K EE ee EE TEE SE APPENDICES Chemical PTFE Borosilicate Glass CTFE Kel F Per en S ee E ve ae ebe a ee S ial na EE Fr EE e DEE E eee er nn EE DEE EE NE EN S BEE EE S A SE SE A idee i he KG EE S EE EE S EE EE SE ae tee S SEET SE EE Ge SE S Kee i fl Ne Je ENE AEE EN en AE ed Jer eee A nee a EN nna SE ee EE GE SE GE S vr EE SEN EE EEN EE S Fe er S SE EE a A e EE S EE P E A EE SS EE SE laa S RE EEE EEN ess ae EN S ee EE Microlab 300 User Manual 107 APPENDICES Appendix D Instrument Performance Test Reports The Microlab 300 is shipped with two calibration certificates that validate the instrument successfully completed the gravimetric testing per ISO 8655 CERTIFICATE OF CALIBRATION 108 Model Tips Used Part No Liquid Type Technician Balance Make Balance Mo
13. hardware settings software settings security settings log settings and user accounts Utilities Press to prime the instrument move the syringes view logs transfer error logs and update the system Pipette Press to enter the Run screen for the Pipette Wizard CHAPTER 10 ICON INDEX Icon Function Serial Dispense Press to enter the Run screen for the Serial Dispense Wizard Configuration Menu Icons Hardware Press to select and configure the button mapping on the Disposable Tip Hand Probe Software Press to review and modify the date time screen calibration screen power off settings internal storage usage Wizard versions and pump versions Security Settings Press to review and modify user accounts required max login retries key life soan and key expiration warning This will only be used if Security is enabled Log Settings Press to review and modify electronic signatures enforce adherence to the Linked Method order view log path select methods to log and set a custom Controller name Ed Microlab 300 User Manual 73 CHAPTER 10 ICON INDEX Function Users Press to edit add export or import users to the instrument Utilities Menu Icons Log Viewer Press to view and print logged methods Error Log Press to transfer error log files to the SD Card This will be used for diagnostic purposes at Hamilton Company Update Press t
14. 1 mL tip to ensure the pipetting accuracy of each dispense Microlab 300 User Manual 59 CHAPTER 6 WIZARDS 6 6 Serial Dispense Wizard This Wizard is used to repetitively dispense aliquots of differing P volumes at the press of a button The total volume which is the sum of all the serial dispenses is drawn into the ClickSure tip and then multiple dispenses are performed as specified The current dispense volume is controlled by the Parameter Adjustment button The current dispense volume can be changed at any time The Edit Steps button shows how many dispenses have been completed out of the total number of dispenses that are programmed In this Wizard the system will also aspirate an additional amount of sample volume 5 uL when using a 50 uL tip and 25 uL when using a 1 mL tip to ensure the pipetting accuracy of each dispense Edit Steps This is the entire list of programmed dispenses that can be edited by pressing the Parameter Adjustment button This will bring up the programmed dispense volumes These volumes may be modified deleted or additional volumes may be added Hold The Serial Dispense Wizard dispenses volumes from the Edit Steps one at a time in order from the first dispense to the last When the last dispense is reached it starts over with the first dispense volume Enabling the Hold functionality causes the current dispense to be dispensed repeatedly until the Hold is disabled at which time the Se
15. A series of two strokes aspirate and dispense that comprise both the up and down movements of the syringe drive mechanism Default A factory setting for example a default aspirate soeed for each syringe size Delete To remove a method or file Delete button This button removes one character at a time from the value indicator or removes a method or file Density Defines the mass of the fluid per unit volume Density is measured in g mL Diluent A liquid that is added to a sample to decrease the sample s concentration Dilute To reduce the concentration of solutes in a sample by adding another fluid The fluid that is added is called diluent and may be deionized water for example Dilution Wizard A Wizard designed to allow the user to perform a dilution where the diluent and sample volumes are not greater than 1 mL Discrete The Discrete functionality in the Serial Disoense Wizard allows the user to aspirate and dispense each volume in the Edit Steps one at a time rather than aspirating the total volume of dispenses Microlab 300 User Manual 411 GLOSSARY Dispense To distribute fluid in a single portion or in several increments from a ClickSure tip on the Disposable Tip Hand Probe Dispense speed Defines how quickly the fluid is dispensed Slower speeds may be used for viscous fluids to allow them to flow to the end of the tip increasing dispense accuracy Display screen The touchscreen on the Controller th
16. AA 43 45 113 Keypad toggle E 45 113 L Linked Methods NING DE 62 63 END ve 65 EE eo A EE A E 62 o o 63 64 Liquid Classes o EE 48 49 FN 49 122 OVIA 49 FINN Eli a 45 47 e e A 48 Ess kN 54 67 Ec STING JE E 32 54 73 Biere 47 54 55 67 M Maintenance TS TUNG A ee e OO e 85 87 EI 81 Nr 87 VEE EELER 81 SI EEEO OO ee 81 Manual Conmventons 9 Method Linker naaa oia 61 65 0 EEE A E E 58 77 114 MOVE SII Straits 32 67 74 N Number of pre wet cycleS EE 46 47 114 NPA 46 114 NET AA 43 114 O Ordering information ccccccceecceeecceeecceeeceseecueeseeeeeeesaees 101 104 BJELLE o EE N 19 20 115 Overview of the Microlab 200 11 14 Microlab 300 User Manual 123 Parameter adjustment button occocccocccccconcccnconocnnnanioncnanons 39 115 Parameter indicator n nannanonnannennnnnnonnnnrnnrnnnnnnnnnrnnnnrnrennnne 40 115 Performance test reports cooccoccconccccconiccnconincncononnnnaninncnanons 108 TL 41 Pipette Wizard MEN KNE 58 72 115 OU CO de E 41 Post aspirate della 46 115 Post blowout ge E 46 116 Post dispense dual Orina 47 116 Post dispense delay ooccconcconoconocononononenenonenaninacinnconncnanenan 47 116 Power on off ewitch 18 gt 50010 1 0 gt senne eeo E sa Ened ear EEE 46 116 ao on 28 32 67 72 74 116 Probe OIE cito 16 22 24 102 116 Probe EN 18 116 R RE 110 190 te JE secon ndee ee rnei ors EEEn 44 117 Replacement parts
17. Cable Management Gvetemm 102 Cable Management System Tumbecrews es 102 SES UDS E 102 Disposable Tip Hand PONG soria gege 102 FN 102 FOWET eil E 103 KOSE e ie NE 103 124 Syringe Drive thumbscrews ccccssecseeecseeseeeeseseeeeeeeaeees 101 NS EE 101 MAL 101 EE EEE 101 Returned Material Authorization RMA number 117 Reverse Pipette Ward 59 117 UTE 39 117 S SE A o EEE E EE EA 11 14 SEE A E EET YO o Rate EE 35 S BES e PEE E EE T A EAE A EE E A 16 53 101 117 Security Sets EE 32 53 73 Serial Dispense Ward 60 73 117 SM DUNO arane ENE 45 118 TUNE 39 40 75 79 118 Software configuration Bae ne fl EE 50 LO q EE 50 51 Syringe Drive E eege 118 System ee E110 os sssrini raissa AEROS 53 MO EE 53 leie NNN 53 Microlab 300 User Manual 125 Technical SPCC CANONS E 98 E eine ET 0 o SEN 96 MT 10 118 MMA 34 36 118 Touchscreen interface A 51 52 i 10 LE 119 Troubles E ar 94 95 Tubing Jace lte BE 20 27 o A 101 U Universal power Supply occooccoccconcoccconoccccononcnnononnnnanonnonanons 16 103 Updating the AV SUITS AT scarico 68 119 BEN ON necia tata 19 20 User interface rates 32 Users e RE 55 o E 55 ENN 55 Bet 66 68 V ENN 44 45 119 VEG 88 119 yae ACU e EEE 17 88 119 WBC receptacle E 18 19 119 VU 119 126 W Wizards ee dE 59 Rule A 59 e 5 eae ok rete eee 58 Reverse Pipette EE ito 59 Serial Uuepenmee 60 Microlab 300 User Man
18. Memory 5 4 7 System Trace Logging 5 4 8 System Calibration Date 5 4 1 Setting the Date and Time To set the Date and Time the user must press the Configuration button from the Main Menu screen and then the Software button From here the user will need to press the Controller View button Using the toggle buttons the user can highlight and select Date and then use the change buttons to set the appropriate date Likewise this process may be repeated for setting the Time 5 4 2 Setting the Elevation As discussed in Section 5 2 2 the Microlab 300 ships with three standard Liquid Classes for pipetting aqueous volatile and viscous solutions The Microlab 300 standard Liquid Classes were developed under factory conditions at 1 300 meters above sea level However as altitude increases and air pressure decreases the volume aspirated by the Microlab 300 syringes is reduced Thus the instrument must be calibrated at ambient air pressure to accurately aspirate and dispense the defined volume The three standard Liquid Classes can be adjusted to compensate for the difference in elevation at a customer site Within the Controller screen in the Software configuration menu a user can input the elevation of his laboratory measured in meters The three standard Liquid Classes will automatically be adjusted based on the new elevation 50 CHAPTER 5 CONFIGURATION MENU Note When creating new liquid classes there is no need to compensate fo
19. Range Indicator displays the valid values for the parameter that is currently selected If a value is entered outside of that range the font color will turn red until an allowed value is entered 5 1 8 Alphanumeric Keypad This screen appears when changing an alohanumeric value The screen initially displays the current parameter value Pressing a keypad button automatically clears the current value Favorite Filename Value Indicator Keypad Buttons Delete Button Shift Button Keypad Toggle Value Indicator This indicator displays the current value that will be stored when the Save button is pressed 44 CHAPTER 5 CONFIGURATION MENU Keypad Buttons These buttons are used to add or clear the numbers or characters from the Value Indicator Shift Button This button toggles the letters from lower case to upper case Keypad Toggle This toggles the keypad between letters and numbers Delete Button This removes one character at a time from the Value Indicator 5 2 Liquid Classes The Microlab 300 instrument allows for the use and creation of Liquid Classes Liquid Classes are designed to compensate for the liquid s density viscosity surface tension and or vapor pressure to maintain accuracy and precision In the software users may use the Liquid Classes that come standard on the instrument or create unique Liquid Classes to get the best pipetting accuracy for the given liquid of choice This section
20. Replacement 11 7 3 Tubing Replacement 11 7 4 Battery Replacement 11 7 5 O ring Replacement 11 7 1 Valve Replacement In this section the user will learn how to properly replace the valve onto the Microlab 300 Valve Shaft Insert Valve Stem Valve Actuator Figure 11 1 Microlab 300 Valve Replacement Step 1 Be sure both syringes have been removed from the system Refer to Section 11 7 2 for instructions on removing the syringes Step 2 Remove the existing valve The valve can easily be removed from the instrument by simply unscrewing the two screws and pulling the valve off the instrument Step 3 Insert the new valve shaft into the valve actuator and rotate the valve until the valve stem engages with the valve drive See Figure 11 1 88 CHAPTER 11 CARE AND MAINTENANCE Step 4 With the valve stem and valve drive engaged rotate the valve until the alignment pins slip into the front of the instrument The threaded syringe ports should point down toward the syringe drive mechanism Step 5 Install the valve screws to complete the valve mounting The final assembly of the valve is depicted in Figure 2 12 11 7 2 Syringe Replacement In this section the user will learn how to properly replace the syringes on the instrument Step 1 Power the Microlab 300 on using the Power On Off switch Step 2 Press the Utilities button on the Main Menu screen and select the Move Syringes button In
21. The VDC receptacle is located in the lower right corner on the back of the Syringe Drive Unit The port is used to connect the power cord to the Microlab 300 system 2 2 3 Description of the Rear View of the Controller VDC Receptacle Ethernet RJ 45 OTG USB Controller Receptacle USB Port Figure 2 3 Rear View of the Controller VDC Receptacle The VDC receptacle is located on the back of the Controller This port is used for charging the Controller It is only used when the Controller is not attached to the Microlab 300 Syringe Drive Unit Microlab 300 User Manual 19 CHAPTER 2 INSTRUMENT SETUP Note A Controller power supply is not shipped with the Microlab 300 because the Controller receives power through the Controller receptacle when connected to a powered Syringe Drive Unit OTG USB Port The OTG USB port is located on the back of the Controller This port is used for diagnostics and testing at Hamilton Company prior to shipment USB Port The USB port is located on the back of the Controller This port is used for connecting the Microlab 300 to the Printer Kit p n 61500 04 Controller Receptacle This port is located on the back of the Controller This port is used for connecting the Controller to the Syringe Drive Unit from which it receives input power Ethernet RJ 45 The Ethernet receptacle is located on the back of the Controller This port will be used for future expansion of the system 2 3 Selecting
22. dispensed In Pipette and Dilution Wizards a blowout can either be triggered after dispensing the liquid or can be set to occur automatically In all other pipetting Wizards the extra volume remaining in the tip after dispense can be removed by selecting Empty Tip on the Run screen CHAPTER 9 BEST PIPETTING PRACTICE 9 4 Pipetting Highly Viscous Solutions Use the Reverse Pipetting Wizard for viscous solutions when using the Viscous300Default Liquid Class or when pipetting plasma serum or other liquids with high protein content Use a filtered ClickSure tip Set Liquid Class parameters to use slow aspirating and dispensing speeds Set Liquid Class parameters to use an aspirate delay to allow liquid to completely enter the ClickSure tip Set Liquid Class parameters to use a post dispense delay or a pre blowout delay to allow the fluid to collect at the end of the tip before being blown out Wipe the tip against the container wall to remove any liquid which may be present on the outside of the ClickSure tip Microlab 300 User Manual 74 CHAPTER 10 ICON INDEX CHAPTER 10 Icon Index This chapter will define the function and purpose of the major icons used throughout the user interface 72 Main Menu Icons Linked Method Press to create a Linked Method Linked Methods are created by linking multiple Wizards or Favorites together to create one run file Configuration Press to set Liquid Class
23. for avoiding personal injury Is flagged with the International Warning Symbol Biohazard Information that alerts the operator to situations where special care IS required to remain protected from chemical or biochemical hazards Sr Important Information that is essential for avoiding damage to equipment Note Interesting information or information that can help improve system performance Microlab 300 User Manual 9 CHAPTER 1 INTRODUCTION CHAPTER 1 Introduction This chapter provides a brief overview of the Microlab 300 Series Pipettor including the following discussions 1 1 Introduction of the Microlab 300 Series Pipettor 1 2 Safety Precautions 10 CHAPTER 1 INTRODUCTION 1 1 Introduction of the Microlab 300 Series Pipettor The Microlab 300 is a precision liquid handling device designed to pipette volumes of liquid from 0 5 to 1 000 uL The system s syringes and valve work together to aspirate and dispense fluid according to the air displacement principle while the single pipette probe uses ClickSure tip technology to ensure a tight seal for optimal pipetting results Finally the Microlab 300 is controlled by a large touchscreen interface which guides users step by step through complete pipetting protocols This instrument comes complete with the Syringe Drive Unit Controller SD card valve syringes tubing hand probe and Cable Management System Chapter 2 will discuss the proper installation of Mi
24. instrument used to turn the Microlab 300 on and off Post aspirate delay Defines the amount of time the system waits after an aspiration to allow the system to reach mechanical and pressure equilibrium The operator is expected to maintain the tip within the fluid during this time interval Longer delays may be needed to adjust for faster aspirate speeds and or more viscous fluids Microlab 300 User Manual 415 GLOSSARY Post blowout delay Defines the amount of time the system waits after dispensing the blowout and before re aspirating the blowout volume in preparation for the next pipetting activity Post dispense audible If enabled an audible buzzer will indicate that a dispense is complete Post dispense delay Defines the amount of time the system waits after a dispense to allow the system to reach mechanical and pressure equilibrium The operator is expected to maintain the tip in a proper dispensing position during this time interval Longer delays may be needed to adjust for faster dispense speeds Pre blowout delay Defines the amount of time the system waits after dispensing fluid and before dispensing the blowout Pre wet This is a step setting that allows the user to aspirate and dispense a solution x times to pre wet the pipette tip that is to be used in the step This improves the accuracy of the dispense Prime The act of making the Microlab 300 ready for operation by running fluid through the fluid path tubin
25. maintenance on the Microlab 300 Microlab 300 User Manual 11 CHAPTER 1 INTRODUCTION When transporting the Microlab 300 for repair or shipment it should be properly packaged inside the original shipping container All Microlab 300 instruments returned to Hamilton Company for repair must be decontaminated before packaged for shipping Only the original approved parts and accessories may be used with the Microlab 300 Any alterations or modifications to the instrument may be dangerous and will void the warranty 1 2 2 Operating the Microlab 300 Series Pipettor When using the Microlab 300 Good Laboratory Practices GLP should be observed During the operation of a Microlab 300 remain clear of moving parts Never try to remove valves syringes or tubing when the Microlab 300 syringe drive mechanism is moving Never move the Microlab 300 while it is in operation lf a spill occurs power off the instrument and wipe it down with the appropriate disinfectant or chemical cleaner Remember to analyze the aspects of the spill to determine what safety precautions should be followed to prevent future spills A Important There are openings at the bottom of the syringes on the front of the Syringe Drive Unit Prevent fluid from entering through these holes as this could damage the internal system electronics 1 2 3 Electrical The Microlab 300 must be turned off and disconnected from the power source when removing any mechanical or electric
26. of the Accept button dispenses only the defined sample volume leaving the additional volume in the tip If the user chooses to repeat the Reverse Pipette function the additional volume will not be re aspirated This volume can be removed from the tip by selecting the Empty Tip icon at the bottom of the Wizard Run screen 6 4 Dilution Wizard This Wizard is used to dilute sample with diluent up to 1 mL total volume Once the system is primed pressing the Accept button causes the defined amount of diluent to be aspirated into the ClickSure tip A Second press of the Accept button causes the defined amount of sample to be aspirated in the tip A third trigger of the Accept button will simultaneously dispense both diluent and sample into the desired vessel The user may also add an Air Gap between the sample and the diluent to ensure that mixing does not occur prior to dispensing 6 5 Aliquot Wizard This Wizard is used to repetitively dispense aliquots of the same volume at the press of a button The total volume which is the sum of all the aliquots is drawn into the ClickSure tip and then multiple dispenses are performed based on the volume selected The volume of each dispense is controlled by the Parameter Adjustment button The number of aliquots dispensed Is indicated on the screen of the Controller In this Wizard the system will also aspirate an additional amount of sample volume 5 uL when using a 50 uL tip and 25 uL when using a
27. parameters including the Liquid Density and speeds should be set appropriately for instrument calibration using deionized water Step 6 Select the Number of Replicates that you will pipette at each volume point It is recommended that you select a number of replicates between 3 and 5 Step 7 Select the tip size and type you would like to calibrate Press Edit Once again all parameters for the Liquid Class should not need to be modified as described in Step 4 Note If pipetting over the complete volume range of the Microlab 300 it will be necessary to perform the calibration routine with both tip sizes once for the 50 uL tip and once for the 1 mL tip Calibration will also need to be performed for each tip type non sterile sterile or sterile filtered intended to be used with the Liquid Class 82 CHAPTER 11 CARE AND MAINTENANCE Step 8 Press the Data Points icon in the lower right corner of the screen All data points will be pre populated from the duplication of the original curve Step 9 Add or delete volume points using the icons at the bottom of the screen For instrument calibration it is recommended that you use a minimum of the volume values indicated in Table 11 1 You may also select volume values that are most commonly used in your pipetting applications Table 11 1 Data Points for Instrument Calibration Tip Size Volume uL Percentage of Tip Sa EE Ge EE Se EE E iid cade KEE EE EE Ee Se SE E i E
28. the Move Syringes screen you may choose to raise or lower the syringes Select lower syringes The syringe drive should move down Step 3 Carefully remove the syringes by turning the thumbscrews at the bottom of each syringe counter clockwise Then turn the glass barrel of each syringe counter clockwise WARNING Be careful not to break the glass of the syringes during syringes removal or installation Broken glass could result in bodily harm Step 4 Insert the 1 25 mL syringe on the left side of the valve and turn the glass barrel clockwise until it is finger tight Tighten the thumbscrew at the bottom of the syringe to secure Step 5 Carefully insert the 25 uL syringe on the right side of the valve and turn the glass barrel clockwise until it is finger tight Tighten the thumbscrew at the bottom of the syringe to secure Microlab 300 User Manual 89 CHAPTER 11 CARE AND MAINTENANCE Ui I UN MN a an Wil WU I H WI With di H N SE d LAAT in ci 00000 000 l First mount the threaded connection IDN HDD Then mount the Thumbscrew Figure 11 2 Replacing Syringes on the Microlab 300 A Important Always tighten syringes so that they are finger tight Syringes that are over or under tightened can cause problems with your Microlab 300 Pipettor Syringes that are over tightened may ca
29. the Proper Location When selecting a location for your Microlab 300 choose an area that is clean dry level and away from hazardous chemicals radiation and or hazardous biological substances Leave enough space around the unit for ventilation Make sure there is a power source nearby to connect the power cord 2 4 Charging the Controller Upon receiving a system it is recommended to charge the Controller Step 1 Connect the provided power cord to the power supply unit Step 2 Plug the power cord into a wall outlet Step 3 Insert the power supply into the Controller VDC receptacle The Controller powers up and displays the Main Menu Charge the Controller overnight prior to use Ensure the temperature of the Controller charging location remains below 35 C 95 F 20 CHAPTER 2 INSTRUMENT SETUP 2 5 Installing the Controller and Syringe Drive Unit The Syringe Drive Unit is the core of the Microlab 300 Series Pipettor It contains a precision drive motor the syringe drive mechanism the valve assembly the power button and all cable receptacles The Controller contains the software necessary to communicate user inputs on the touchscreen like volume to the Syringe Drive Unit resulting in precision pipetting This section describes the installation of the Controller and Syringe Drive Unit Ke I Controller Migros J FUZANENA i DNI
30. 3 Cleaning the Exterior of the Microlab 300 11 5 4 Cleaning the Disposable Tip Hand Probe 11 5 1 Deciding When to Clean the Microlab 300 Cleaning the Microlab 300 is dependent on how the instrument is used with respect to the duration of use and types of solutions being used by the system We recommend that you clean the instrument daily The tubing and syringes should be purged and primed at the end of each experiment and or each work shift To clean the Microlab 300 flush the system with ethanol or with 10 chlorine bleach and deionized water solution Avoid using caustic or acidic cleaning solutions Microlab 300 User Manual 85 CHAPTER 11 CARE AND MAINTENANCE 86 11 5 2 Cleaning the Fluid Path For highly critical applications the Microlab 300 should be cleaned on a regular basis Use ethanol or 10 dilute chlorine bleach to clean the fluid path See Appendix C for chemical compatibility with the Microlab 300 Steps on cleaning the fluid path are listed below When the tubing and syringes come in contact with contaminated or hazardous samples follow safe laboratory practices in selecting and using a cleaning fluid to flush out the tubing and syringes WARNING Follow laboratory safety procedures if the Microlab 300 instrument comes in contact with hazardous materials Step 1 Prepare a reservoir of cleaning fluid and place the fill tubing in the cleaning fluid reservoir Step 2 Direct the hand probe into the cleaning flu
31. AAAAAAAAAOAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA K KS 102 APPENDICES Table B 7 Power Supply r Description Part Number Power supply 61092 01 Table B 8 Replacement Power Cords Description Country Diagram of Plug Part Number Standard CEE 7 7 Senn ena EUOPS 3892 01 Russia Schuko Standard AS 3112 Pda oe 3892 02 Argentina China UK Ireland Malaysia Standard BS 1363 Middle East 3892 03 USA Canada Mexico Standard NEMA 5 15p Genial Ameren Brel Y 3892 05 Microlab 300 User Manual 103 APPENDICES Table B 9 Accessories r 5 Description Part Number Cable Management System 65160 01 Tubing Clips 5 pack 88990 p Table B 10 User Manual r NI Description Part Number Microlab 300 User Manual 67258 01 4 104 APPENDICES Appendix C Chemical Compatibility This section contains information about chemical compatibility with the Microlab 300 instrument at room temperature The fluid path consists of the inside syringe barrel which is made of borosilicate glass the tip of the syringe plunger which is made of PTFE and the internal valve components which are made of PTFE and CTFE LEGEND 0 No data available A No effect excellent B Minor effect good C Moderate effect fair D Severe effect not recommended d I Chemical PTFE Borosilicate Glass CTFE Kel F O O el A A ET EE S RE EE ee ee O EE oo See EE EE
32. CING THE MICROLAB 300 SOFTWARE 3 1 User Interface Flow Chart The flowchart below shows the interrelationship among the major screens in the Microlab 300 Series Pipettor user interface The Main Menu screen provides access to all other screens within the user interface Press the Icon buttons on the Main Menu screen to navigate through the interface Press the Back button to return to the previous screen Prime a Method Liquid Reverse Pipette Hardware Linked Method Editor Linked Method Run Move Syringes E i O Viewer Security Settings Error Log Lo Settings Serial Dispense Update 32 CHAPTER 4 DISPOSABLE TIP HAND PROBE CONFIGURATION AND OPERATION CHAPTER 4 Disposable io Hand Probe Configuration and Operation This chapter discusses details on the Disposable Tip Hand Probe and will cover the following topics 4 1 Configuring the Disposable Tip Hand Probe 4 2 Operating the Disposable Tip Hand Probe Microlab 300 User Manual 33 CHAPTER 4 DISPOSABLE TIP HAND PROBE CONFIGURATION AND OPERATION 4 1 Configuring the Disposable Tip Hand Probe The probe buttons can be set for right handed or left handed use within the Configuration menu of the Microlab 300 Controller Step 1 Select Configuration from the Main Menu screen Step 2 From the Configuration screen select Hardware Step 3 Choose whether the probe will be used in the right or left hand mode e Probe Tubing Probe Cab
33. E Ee i Pr The data for accuracy and precision only applies after instrument calibration using Hamilton ClickSure pipette tips ud 100 Appendix B Replacement Parts and Accessories Table B 1 Replacement Syringes Description Part Number cl a ad Ke ke ian ee Ke ar Thumbscrew 1 pack 66829 01 Ee Table B 2 Replacement Controller Parts Description Part Number Controller cable 66063 01 Microlab 300 SD card 67255 01 VE Table B 3 Replacement Valves r Description Part Number Microlab 300 Valve Assembly 66008 01 Table B 4 Replacement Tubing Description Part Number 12 gauge fill tubing 61614 01 Microlab 300 User Manual APPENDICES 101 APPENDICES Table B 5 Disposable Tip Hand Probe and Tips Description Part Number saad A A An GE E ane EA A an ege Ke EE E EE Ce ne SE EE SE SE EC E EE EE a aa ede can ae apr area ge e SE AS T BEEN SEN SSES REESEN a 8 SS SS i e EE S sene EE EE Se E E Gg 9 EEN BEN ease PR a a o OA DoS E E SE Een ES A ae en 1 Includes 5 ferrules 5 stainless steel rings 5 14 28 fittings 5 strain reliefs 12 gauge tubing and a probe tip Table B 6 Microlab 300 Cable Management r Description Part Number nope ae supe ieee RE e EE ms See KE ET a GC EE seco EE oot SE E E e Thumbscrew 1 pack 65164 01 2 AAA AAAA
34. E EE E As i FE saison ds Gaia EE i 1 2 Note In the data points screen there will be a high and low volume point for each tip size that cannot be modified For example the 50 uL tip has a high point of 50 uL and a low point of 1 uL When creating the calibration curve these high and low volume points will be used Calibration will also need to be performed for each tip type non sterile sterile or sterile filtered intended to be used with the Liquid Class Now the system can be gravimetrically calibrated for the selected tip size and tip type 11 4 2 Calibration using the Liquid Class Wizard Step 1 After adding or deleting volume points as described in Step 9 of the Calibration Routine select the volume value that you would like to begin calibrating Step 2 Select the Wizard icon in the lower right corner of the Liquid Curve Data screen You have now entered the Liquid Class Wizard Run screen The number of replicates for each volume value will be indicated by the number of lines in the table Microlab 300 User Manual 83 CHAPTER 11 CARE AND MAINTENANCE Step 3 Place a plastic or glass weighing container on the balance and adjust the weight tare to zero Note The recording of the weighing results requires the use of a precision balance with a high resolution Dispensing operations under 10 uL require a balance with a resolution of 0 001 mg Step 4 Load the ClickSure tip type and size being used for calibr
35. Management System oocccoccncnccnoncnnncnnononcononononenoss 22 2 7 Installing the Disposable Tip Hand Hrobe 24 2 8 Installing the Fill and Probe Tubmg 26 2 9 Applying Power to the lnetrumenmt 28 Ac lt A 28 2 11 Using the Close TIDS datos 29 2 12 Installing the Printer Optional ooccocccocnoocnoncnoncnoncnoncnononononononononons 29 CHAPTER 3 Introducing the Microlab 300 Goftware 31 21 User o NM 32 CHAPTER 4 Disposable Tip Hand Probe Configuration and Operation 33 4 1 Configuring the Disposable Tip Hand Drobe 34 4 2 Operating the Disposable Tip Hand Drohbe 35 CHAPTER 5 Configuration Men 37 5 1 Overview of Screen Terminology ccccsececseecceeeceeecseecseeeceueeseseeeesaeess 38 ll COMO E 38 Se EE o o PI ee ere es 39 Sl Data Eny NN 40 12 PN 41 og ae E EEE 41 5 1 6 Linked Method Screens occoocccocooncconcnoncnonnnonnnonconoccnncnnononrnnnrnenononons 42 9 NT SG e eins aaa ria eran 43 518 ANNENS Pee Ai 44 4 Me 010 GE een 45 521 Louia Class En KT EE 45 5 2 2 Standard Liquid Ee 48 5 2 3 Creating Liquid COaseee 48 5 2 4 Editing Deleting Liquid COasees 49 525 IMPO EO LOU We EE 49 5 3 Hardware e ele e HON EE 49 54 oware 10011610 o oO 0NUU 0CO E E lt P 50 5 4 1 Setting the Date and me 50 E E dl ere todita ett slcis doin 50 5 4 3 Calibrating the Controller Touchscreen oocccocccoccconnconcconnconononnnnnos 51 5 4 4 Screen Power Off nina ctas 52 5 4 5 Firmware Software Verslons ccoccc
36. ON Check the power supply to see if the light is on when plugged in Controller unit not plugged into Check to see that the Controller unit is the Syringe Drive Unit plugged into the Syringe Drive Unit Instrument functions SEET SE normally but display Plug power supply directly D s ns seen Controller If the Controller illuminates Faulty pump circuit board then there is an internal problem illuminate with the Syringe Drive Unit Call the Hamilton Service Department Faulty Controller cable Call the Hamilton Service Department Power adapter is plugged into the Syringe drive is Controller and not the Syringe So Ne SS SECHS non responsive and Drive Unit UNG EDIE Controller displays Device Is not connected Controller cable is connected into the Accessory port and not the Controller port on the Syringe Drive Unit Check tubing valve and syringes for Faulty or blocked tubing blocked a blockage crimps or loose fittings or leaky fluid path replace tubing either replace valve and syringes or tighten valve and syringes Plug the Controller cable into the Controller port of the Syringe Drive Unit Instrument does not fill or dispense Non functional Syringe Drive Unit Call the Hamilton Service Department Valve does not rotate valve drives are not engaged or valve is faulty Power is applied to Controller Plug the power supply into the Syringe and not the Syringe Drive Unit Drive Unit
37. Provide a complete description of any hazardous materials that have been used with the instrument Biohazard Hamilton Company reserves the right to refuse a return shipment of any Hamilton product that has been used with radioactive microbiological substances or any other material that could be hazardous to Hamilton employees Important Do not return syringes tubing or valves with your instrument Hamilton Company will assume that they pose a health hazard and will destroy them Microlab 300 User Manual 97 APPENDICES Appendices Appendix A Technical Specifications Table A 1 Technical Specifications 98 KE Problem Possible Causes re Sn ES SEENEN EE E Wa a e EE EE o A S EC ebe EH EE Ee e e o REO AAO E Gg ver ee ae ee E SE SS A Een KE on A AAA E N E E a WEE DEN REENEN WEE ies 100 O E EEE T a ogre EE a NESE E ee E a a sory EE ees A O o naa ee a AA E neon EE Proa DEE ae age SE NG NS ag cere Se Pre AAA AAA E IEI E mr BE WEE REENEN 7 o SE Ge EE KEE Seege E eege EE Seen Re NN EN Indoor Operation and Use Only 1 only for the instruments that are designated for IVD 2 Per Section 3 6 6 2 pollution degree 2 is defined as normally only non conductive pollution occurs addition of foreign matter solid liquid or gaseous ionized gasses that may produce a reduction of dielectric strength or surface resistivity Occasionally however a temporary conduct
38. Volatile Developed using 99 5 isopropyl alcohol Default300Viscous Developed using 87 mg mL Bovine Serum Albumin BSA 5 2 3 Creating Liquid Classes Step 1 To create a new Liquid Class enter the Configuration menu from the Main Menu screen Then select Liquid Classes Step 2 Select one of the standard Liquid Classes that is closest to the properties of your liquid Step 3 Press the Duplicate button name the new Liquid Class and press Save Note Duplication will copy all of the pipetting parameters of the selected Liquid Class including pipetting speeds blowout properties and aspiration or dispense delays etc It will also duplicate the points of calibration volume and mass used to create that liquid compensation curve After duplicating a Liquid Class Hamilton recommends checking all parameters and data points and modifying them accordingly Step 4 After the Liquid Class is created press the Edit button next to the Liquid Class that was created This will take the user to the Liquid Class settings screen Step 5 Enter the Liquid Density g mL and Number of Replicate parameters by highlighting the parameter and inputting the appropriate value Step 6 Select the tip size and type that you want to modify by pressing the Edit button Enter the appropriate values for the additional parameters The additional parameters include Pre Blowout Delay Post Blowout Delay Aspiration Speed Post Aspirate Delay Dispense Speed P
39. added to provide details to the users during the run e g pipette Solution A Once created a Linked Method becomes a run only protocol Users will not be able to modify any of the parameters when running the method 7 2 Creating Linked Methods Step 1 Select the Linked Method icon from the Main Menu screen Step 2 Press the New button in the lower right corner Input the name of the method and press the Save button Step 3 To select one of these options press the button next to the application and select Accept 62 CHAPTER 7 METHOD LINKER Note If a step is added that is not needed it may be selected and deleted If the steps are not in the correct order a user may move any step by first selecting the step and using the left and right arrows to move the step to the proper location Step 4 To change the parameters of a step select the step The user is notified that the step is selected when the step becomes highlighted in blue Step 5 Press the Edit button The user will be able to add notes to the method by selecting the Information button loop a step in a method n times select the volume to dispense select the tip size set the Liquid Class set the tip type and set the pre wet and mix settings Repeat this with all the steps in the method A Important If a user has already created a Favorite of any Wizard it is possible to use the Favorite as a step in a Linked Method rather than recreating the parameters Sele
40. al components A Important The power supply provided with the Microlab 300 is the only power source approved for connecting to the instrument Unapproved power supplies can cause overheating burning melting and short circuiting of the device The use of so called substitute or alternative power supplies is prohibited Also ensure the power source is plugged into a power outlet having the voltage and frequency that matches the power supply input requirements Check to ensure the appropriate power cord for your country was received See Appendix B for appropriate power cords Only connect the Microlab 300 power supply to a grounded outlet 12 CHAPTER 1 INTRODUCTION Charge the Controller overnight prior to use Ensure the temperature of the Controller charging location remains below 35 C 95 F Avoid damaging the power cord while operating the instrument Do not bend it excessively step on it or place heavy objects on the power cord A damaged power cord can easily become a shock or fire hazard Never use a damaged power cord 1 2 4 Biohazard If the Microlab 300 becomes contaminated with a biohazardous or chemical material it should be cleaned in accordance with the maintenance procedures See Chapter 11 Care and Maintenance Section 11 5 Observe and perform the given maintenance procedures Failure to do so may impair the reliability and correct functioning of the Microlab 300 If working with biohazardous
41. and view method logs Learn more about the LyncStore at www hamiltoncompany com 8 4 Transferring Error Logs The Error Log screen provides the means to transfer all error logs from the instrument onto the Microlab 300 SD card by pressing the Run button These files can then be sent to Hamilton for diagnostics Microlab 300 User Manual 67 68 CHAPTER 8 UTILITIES 8 5 Updating the Instrument Firmware and Software The Update screen allows the user to update the instrument to the latest firmware and software The update will be downloaded from the website onto the SD card The SD card can then be placed in the instrument By selecting Update from the Utilities menu the instrument will receive the newest firmware and software updates CHAPTER 9 BEST PIPETTING PRACTICE CHAPTER 9 pest Pipetting Practice To achieve the highest precision and accuracy we recommend that you follow the following pipetting tips explained in this chapter including 9 1 Preparing to Pipette 9 2 Aspirating Liquid 9 3 Dispensing Liquid 9 4 Pipetting Highly Viscous Fluids Microlab 300 User Manual 69 CHAPTER 9 BEST PIPETTING PRACTICE 9 1 Preparing to Pipette Always place the liquid in a suitable vessel The liquid and the ClickSure tip should have approximately the same temperature Use ClickSure filtered tips to prevent contamination by aerosols or foaming solutions in the tip Use ClickSure tips only once Check whe
42. at allows the user to program and operate the instrument Disposable Tip Hand Probe The Disposable Tip Hand Probe is the compatible probe for the Microlab 300 The probe accepts two ClickSure tip volume sizes 50 uL and 1 mL Down Describes the movement of the syringe drive mechanism as it moves away from valve Down position This is the position of the syringe drive mechanism when it is fully extended away from the valve Edit steps This Parameter Adjustment button is the entire list of programmed serial dispenses that can be edited These volumes may be modified deleted or additional volumes may be added Ethernet RJ 45 The Ethernet receptacle is located on the back of the Controller This port will be used for future expansion of the system Fluid direction indicator This indicator shows the direction of fluid movement in or out of the ClickSure tip in the next step 112 GLOSSARY Fill tubing A tubing line that connects the fill reservoir to the valve inlet port on the left side of the valve Fluid Path The inside surfaces of the valve syringe s and tubing that contact the system fluid Hand probe See Disposable Tip Hand Probe Hold The Serial Disoense Wizard dispenses volumes from the Edit Steps one at a time in order from the first dispense to the last When the last dispense is reached it starts over with the first dispense volume Enabling the Hold functionality causes the current dispense to be dispense
43. at has been used with radioactive or microbiological substances or any other material that may be deemed hazardous to employees of Hamilton Company 2012 Hamilton Company All rights reserved Compliance This equipment has been tested and complies with the limits for a class B digital device pursuant to Part 15 Federal Communication Commission FCC Rules These limits are designed to provide reasonable protection against harmful interference in an installation This equipment generates uses and can radiate radio frequency energy and if not installed and used in accordance with the instructions may cause harmful interference to radio communications However Hamilton Company cannot guarantee that no interference will occur in a particular installation If this equipment does cause harmful interference to radio or television reception which can be determined by turning the equipment off and on the user is encouraged to try to correct the interference by one or more of the following measures Turn the Microlab 300 off and on to determine whether it is generating interference Reorient or relocate the receiving antenna Increase the separation between the equipment and receiver Connect the equipment into an electrical outlet on a circuit that is isolated from the circuit to which the receiver is connected Consult the dealer or an experienced radio TV technician for help This equipment has been verified to comply with the limi
44. ation onto the Disposable Tip Hand Probe Step 5 Press the Accept button on the probe to pre wet the tip with deionized water Step 6 Press the Accept button on the Disposable Tip Hand Probe to aspirate the defined volume of deionized water Step 7 Press the Accept button on the Disposable Tip Hand Probe to dispense the defined volume of deionized water into the weighing container Step 8 Close the door of the balance and after the mass has stabilized record the mass value Step 9 Repeat Steps 5 8 using the same ClickSure tip for the number of replicates defined Step 10 Eject the ClickSure tip Step 11 Press the Liquid Curve Data button in the lower left corner to automatically average the mass values provided The averaged mass value will be populated into the Liquid Curve Data Step 12 Repeat Steps 1 11 for the additional volume values entered in the Liquid Curve Data screen You have now successfully completed instrument calibration for the tip size and type being used Once again it will be necessary to perform the calibration routine with both tip sizes once for the 50 uL tip and once for the 1 mL tip Calibration will also need to be performed for each tip type non sterile sterile and sterile filtered intended to be used with the Liquid Class After calibration validation per ISO 8655 can be performed Please reference the latest revision of the ISO 8655 specification for the latest procedure To validate the Mi
45. core gt DQ mmm Strain Relief Tubing Figure 11 3 Exploded view of Disposable Tip Hand Probe cable and tubing 11 7 3 2 Installing New Probe Tubing Step 1 Gently thread the tubing through the tip of the hand probe The end without the flange should go into the probe first As the tubing is being pulled through the probe thread the tubing through the tubing clips on the probe cable Pull the tubing until the flange seats inside the end of the probe Step 2 Screw the new probe tip onto the probe and tighten to ensure a good seal Step 3 Place the strain relief followed by the 28 threaded fitting on the non flanged tubing end with the threads facing the end of the tubing Move the fitting down the tubing so it does not interfere with the next steps Step 4 Place the stainless steel ring and the yellow ferrule on the non flanged tubing end The correct orientation of the stainless steel ring is important the thinner edge of the ring should face toward the fitting as shown in Figure 11 3 Note Correct orientation of the ferrule is with the thinner side facing the fitting Microlab 300 User Manual 91 CHAPTER 11 CARE AND MAINTENANCE Step 5 Once all the pieces are assembled onto the tubing wet the fitting using deionized water Screw the fitting into the valve while lightly pressing the tubing into the valve to ensure a flush connection The stainless steel ring will compress onto the ferrule and create a
46. crolab 300 User Manual 81 CHAPTER 11 CARE AND MAINTENANCE Using the density g mL and the mass values of the pipetted fluid at each volume used in calibration the Microlab 300 determines compensation factors The factors are calculated from volume point to volume point for the specific pipetting technique being used The compensation factor is also used below the lowest volume in the range and above the highest volume point in the range to extrapolate additional data points beyond those measured during calibration Hamilton recommends completing a gravimetric test to check the validity of the compensation factors Should you choose to calibrate the Microlab 300 without sending the instrument to Hamilton Company please follow the following calibration routine 11 4 1 Calibration Routine Step 1 Prime the system Reference Section 2 10 for priming instructions Step 2 Enter Liquid Classes from the Configuration screen Step 3 Create a new Liquid Class by selecting the Microlab 300 standard Liquid Class Aqueous300Default and then selecting Duplicate at the bottom of the screen Step 4 Enter a name for the new Liquid Class It is recommended to name the calibration Liquid Class such that it is recognized as a calibration curve For example Calibration220ct2012 Step 5 Find the new Liquid Class on the Edit Classes screen and select Edit Because you duplicated the standard Liquid Class for deionized water Aqueous300Default all
47. crolab 300 follow the instructions below Step 1 Select Wizards from the Main Menu screen Step 2 Select Pipette from the Wizards screen Step 3 Select the Advanced button on the Run screen Then select the Liquid Class and Tip Type settings that you developed during the calibration routine Step 4 Change the Pre wet setting to On Then return to the Run screen 84 CHAPTER 11 CARE AND MAINTENANCE Note The number of pre wet cycles is determined in the Liquid Class If you need to modify the pre wet settings refer to Section 5 2 4 for editing Liquid Class instructions Step 5 Use the parameter adjustment buttons to input the volume and tip size being tested It is recommended to validate each tip size at 100 50 and 10 of each tip s total volume capacity per Table 11 1 Follow the latest revision of the ISO 8655 specification for the number of replicates to perform as well as tip reuse instructions Step 6 After all of the pipetting data has been collected for both tip sizes reference Appendix A Table A 1 to validate that your pipetting results comply within the technical specifications of the instrument Should the results not comply please contact Hamilton Company for assistance 11 5 Cleaning the Microlab 300 This section will discuss how to properly clean the Microlab 300 and will include discussions on the following topics 11 5 1 Deciding When to Clean the Microlab 300 11 5 2 Cleaning the Fluid Path 11 5
48. crolab 300 Series Pipettor components Note The ambient temperature of the instrument location should continuously remain below 35 C 95 F 1 2 Safety Precautions For proper handling and care of the Microlab 300 it is essential that operating and service personnel follow general safety procedures and safety instructions described in this manual Service maintenance must be performed only by authorized service technicians Hamilton Company strongly recommends reading this information before proceeding with the remaining chapters in this manual 1 2 1 General Safety Information The Microlab 300 should be placed in a location where personnel have easy access to the front back and sides for ease of operation and maintenance Before operating the instrument determine the amount of space needed for efficient and safe operation of the Microlab 300 Choose an area that is clean dry level and away from hazardous chemicals radiation and hazardous biological substances Also the area should be free from corrosives Leave enough space around the unit for ventilation Avoid placing the instrument near other instruments or objects that could inhibit or stop air flow or otherwise cause overheating of the electronics Cleaning dismantling and performing maintenance on the Microlab 300 should be performed only by properly trained personnel who know how to avoid possible dangers Only certified repair technicians are authorized to perform mechanical
49. ct the method icon in the sequence that you would like to replace with a Favorite of that method Select the Edit small icon button On the Settings screen select the Favorites icon in the bottom toolbar Finally press Select to choose the desired Favorite Step 6 When the parameters of each step have been modified save the method You will be directed to the list of Linked Methods on the system Note Linked Methods may be duplicated Simply select the Linked Method that you would like copied and press the Duplicate button All parameters will be copied and may be modified by selecting the file and pressing the Edit button 7 3 Running Linked Methods Step 1 To run a Linked Method select the Linked Method icon from the Main Menu Step 2 Select the appropriate Linked Method from the list and press the Run button The Linked Method Overview screen will display indicating the sequence of steps in the Linked Method Microlab 300 User Manual 63 64 CHAPTER 7 METHOD LINKER Linked Method Aliquot Reverse Pipette Serial Dispense Loop 1 Liquid Class Aqueous300Default Diluent Volume 750 Tip Type Standard Sample Volume 250 Pre wet Mode Off Tip Size 1 mL Mix Mode Off Air Gap Trigger Off a Method Selector Step 3 Press the Accept button on the probe to begin the method The user will be presented with the Run screen of the active Wizard Each time the user completes a step in the Linked Method the Linked Method Overv
50. d Classes Aqueous Volatile and Viscous may not be deleted from the instrument or modified They may be duplicated to create new Liquid Classes as described in Section 5 2 3 5 2 5 Import Export Liquid Classes To import Liquid Classes from the SD card or to export Liquid Classes to the SD card the user simply presses the Import or Export buttons on the Edit Classes page and then selects the files that need to be imported or exported The imported files will propagate in the Liquid Classes screen The exported files will be saved on the SD card These can then be transferred onto another instrument or to a PC 5 3 Hardware Configuration The Hardware Configuration allows the user to manipulate the different hardware components that are connected on the Microlab 300 instrument At this point in time there is only the Disposable Tip Hand Probe which needs hardware configuration The Disposable Tip hand probe can be configured to right or left hand use as described in Section 4 1 As more hardware components are developed for the instrument they will be added here Microlab 300 User Manual 49 CHAPTER 5 CONFIGURATION MENU 5 4 Software Configuration The Software Configuration menu allows the user to review and modify software settings 5 4 1 Setting the Date and Time 5 4 2 Setting the Elevation 5 4 3 Calibrating the Controller Touchscreen 5 4 4 Screen Power Off 5 4 5 Firmware Software Versions 5 4 6 SD Card Capacity and System
51. d repeatedly until the Hold is disabled at which time the Serial Dispense Wizard will move to the next dispense Icon buttons These buttons are used to provide the major navigation from function to function within the user interface In vitro Diagnostic Device IVD Devices that are used to perform diagnoses in a controlled environment outside of a living organism Keypad buttons These buttons are used to add and clear numbers and characters from the value indicator Keypad toggle This toggles the keypad between letters and numbers Linked Method The run only electronic protocol SOP comprised of a sequence of Wizards or Favorites Microlab 300 User Manual 113 GLOSSARY Linked Method Editor The program used to connect several Wizards in a Sequence to create a complete pipetting application called a Linked Method Liquid Class A solution setting in the instrument that allows for users to customize their system based on liquid used in the application Main Menu The main screen on the Controller for the Microlab 300 This is the screen used to navigate from and to all other screens Memory The information storage capacity of the instrument Method A complete series of repeatable functions that can be performed by Microlab 300 instruments These functions may consist of pipetting aliquoting diluting etc Mix This is a Wizard in the Linked Method screen that allows the user to mix a sample by aspirating and dispe
52. del Serial Number Barometric Pressure Room Temperature Relative Humidity Liquid Temperature Coeff of Evaporation Rel Liquid Density Volume Setting Dispensed Volumes 1 Average Volume Standard Deviation Mean Error Spec Mean Error Actual Pass Fail Cv Spec CV Actual Pass Fail Certified by NIST Test Numbers Test PASSED 50 HL Microlab 300 Series Pipettor 50 uL non sterile ClickSure Tips 960 racked 67100 01 Serial No MLP300H01005 D I water Work Order 12345 J DOE Sartorius MC 5 70105086 Balance Sensitivity 4 000001 g Calibration Due 11 2012 Test Conditions 87256 22 6 55 2 22 6 0 010 0 996810 Pa Ge sg mg mg L 87261 22 6 56 3 22 6 0 010 0 996810 Pa EG TE 87274 22 6 56 2 22 6 mg 0 010 mg uL 0 996810 Test Data 5 0 ph 046 uL 975 uL 804 uL 005 pL Test Results 5 002 0 158 24 915 0 181 uL uL 1 50 0 04 Pass 0 85 0 34 Pass 0 60 0 59 Pass 1 00 0 96 Pass lt on 25 Oct 2012 822 270236 04 The product specified above has been calibrated at ambient pressure The calibration is performed pursuant to ANSINCSL Z 540 1 1994 with an unbroken chain of calibrations traceable to N LS T HAMILTON OF ENG THE MEASURE ELLENCE Hamilton Bonaduz AG patna CEATIiFi ts 7402 Fax 41 81 660 60 70 Telephone 41 81 660 60 60 P N 2297 01 REV C Figure D 1 E
53. dene B US FE OIG ES o EN E TOE Best pipetting practice nnenanoenunnanonnnnrnnonnnnnnrnnrnnrnennnnnnne C Cable Management System cc cccccssecesseeeseseeeeeeeeseseesaes be Calibrating Microlab 300 Series Fpettor elles EE EE Cancel DUTON hr Change DUTTON tonteria Chemical compatibility EE Cleaning Disposable Tip Hand Hrote nn e o SU A eo o On 120 16 22 24 102 104 110 16 22 24 102 110 INDEX Se SUNS de 29 102 110 CICKSUFE tip volume INGICAION isiisinvivcsicsnnscdessvnisessnsnconaceuraees 40 110 Configuration SCreen sicccsccdscesscexencdcccedieninceveccbaetecdeestescecticomecs 110 Controller Controller roceptoce 18 19 110 Bed jeg EE 16 19 20 D Data entry SCreens ENEE 40 Delete button EE 44 45 111 PEN A 46 111 OT VET 32 59 73 77 111 BIG ee 60 111 Dispense SSC iii s 112 Disposable Tip Hand Probe ENN 87 Beieleit ge E 34 Nr 24 25 PTE 35 36 E A 60 112 A E 32 67 14 ENS OE E EEE 20 112 E FANON EE E 57 4 75 Firmware software versions oocccoocccoccconcconeconecononanonanonaninnnnns 52 53 68 Fluid direction indicator asian 40 112 Microlab 300 User Manual 121 Hardware 0101 gr 01816 10 E 49 leie PENE EE 60 113 COn DUNONG EE 38 39 72 79 113 ICON SE LN 38 Installation Cable Management Gwvstemm 22 24 Disposable Tip Hand Hrobe 24 25 Electrical CONE Sri acts 28 id e A A OU MEA a 29 nn EE NE 89 90 MIT 90 91 VU 88 K
54. describes Liquid Class parameters and how Liquid Classes can be used 5 2 1 Liquid Class Parameters 5 2 2 Standard Liquid Classes 5 2 3 Creating Liquid Classes 5 2 4 Editing Deleting Liquid Classes 5 2 5 Import Export Liquid Classes 5 2 1 Liquid Class Parameters Microlab 300 Liquid Classes include all parameters that a user may adjust to fine tune pipetting accuracy Changes in the Liquid Class will not affect the dispensing precision Precision can be improved by replacing worn parts Microlab 300 User Manual 45 CHAPTER 5 CONFIGURATION MENU Liquid Class parameters include density aspirate soeeds dispense speeds post aspiration delays post dispense delays blow out delays and number of pre wet cycles as described below Density Defines the mass of the fluid per unit volume Density is measured in g mL Number of Replicates Defines the number of times a particular volume will be pipetted and weighed during gravimetric calibration The software will calculate the average mass from these replicates and use this average mass value and the density to adjust the data points of a Liquid Class curve Pre Blowout Delay Defines the amount of time the system waits after dispensing fluid and before dispensing the blowout Post Blowout Delay Defines the amount of time the system waits after dispensing the blowout and before re aspirating the blowout volume in preparation for the next pipetting activity Aspirate Speed Defin
55. e at a lower duty cycle 95 CHAPTER 12 TROUBLESHOOTING 12 2 Getting Technical Support If a problem persists even after attempts to correct it contact Hamilton Company Technical Support or Service Department To expedite service please have the instrument part number and serial number ready and available Please be able to provide application specific information drive speeds and liquids being used In the United States and Canada Hamilton Company Inc 4970 Energy Way Reno Nevada 89502 Customer Service 1 888 525 2123 Technical Support Service 1 800 648 5950 Outside the U S 1 775 858 3000 96 In Switzerland Hamilton Bonaduz AG Via Crusch 8 Ch 7402 Bonaduz GR Switzerland Customer Service 41 81 660 60 60 Fax 41 81 660 60 70 CHAPTER 12 TROUBLESHOOTING 12 3 Returning Instruments for Repair Before returning an instrument to Hamilton please contact Hamilton Company and request a Returned Materials Authorization number RMA Note Do not return instruments to Hamilton Company without an RMA number This number assures proper tracking of your instrument Instruments that are returned without an RMA number will be sent back to the customer without being repaired Biohazard The Microlab 300 instrument MUST be decontaminated before it is returned to Hamilton Company To decontaminate the instrument remove health hazards such as radiation infectious diseases corrosive agents etc
56. e is pressed when a ClickSure tip is discarded from the probe 118 GLOSSARY Trigger To press a button on the Disposable Tip Hand Probe to elicit a response from the instrument Tubing clip A component that attaches to the side of a liquid reservoir to hold tubing lines in the fluid Update This button is used to update the software or firmware of the Microlab 300 instrument Value indicator This indicator displays the current value that will be stored when the Enter button is pressed Valve The component that directs the flow of liquid through the system Valve actuator The valve actuator is part of the Syringe Drive Unit that engages with the valve to rotate the valve to the correct position at the correct time Valve drive motor The valve drive motor is part of the drive unit that controls valve rotation Valve port Openings in the valve assembly through which liquid flows As the valve turns ports are opened or closed directing the flow of liquid through the system tubing VDC receptacle This is the receptacle where the power cord connects to the Syringe Drive Unit to a wall outlet There is also a VDC receptacle on the Controller used to charge the Controller Viscosity A property of liquid describing its resistance to flow Alcohol for example has a very low viscosity whereas glycerin has a very high viscosity Microlab 300 User Manual 119 INDEX Index A Alphanumeric kevpad Aspirate SPE re
57. each screen provide additional functionality to change values on the current icon screen 5 1 2 Run Screens These screens enable control and monitoring of all aspects of the pipettor s activities Buttons and icons are used to change parameters and indicators are designed to give current and future system status Dilution Tip Size Clickaure ENEE Parameter hanes Adjustment ndicator Buttons Diluent Volume Sample Volume Fluid 250 0 ul Direction Soa Indicator Total Volume Dilution Ratio Parameter haear 1000 0 ul MS Pack Buon dl Wizards i Small Icon Buttons Run Empty Tip Favorites Advanced Parameters Adjustment Buttons These buttons show the current dispense setting The exact function for these buttons are defined in greater detail in the Wizards and Linked Method sections of this manual Microlab 300 User Manual 39 CHAPTER 5 CONFIGURATION MENU Parameter Indicator This indicator shows the value of a parameter but does not allow for the adjustment of the parameter Back Button Pressing this button in the lower left of the screen displays the previous screen ClickSure Tip Volume Indicator This indicator shows the current volume of solution in the ClickSure tip Small Icon Buttons These buttons provide additional functionality to the Run screen The exact function for these buttons is defined in greater detail in the Wizards and Linked Method sections of this manual Fluid Direction Indicator This indicator sho
58. ecision Instruments Congratulations on purchasing the Hamilton Microlab 300 Series Pipettor The Microlab 300 is a hand operated semi automatic precision pipetting device that provides accurate liquid handling via one pipette probe over the complete pipette volume range of 0 5 ul to 1 mL The Microlab 300 functions on the principle of air displacement At the heart of each Microlab 300 Series Pipettor is a precision stepper drive motor combined with our uniquely designed Gastight syringes The result is a precise and accurate instrument that is easy to set up and operate Proper handling and maintenance of your new Microlab 300 will increase the lifespan of the instrument To learn about proper care and maintenance of your investment please read this entire manual including the warranty information Hamilton Company thanks you for purchasing the Microlab 300 Series Pipettor INTENDED USE Intended Use This manual applies to both the Microlab 300 and the Microlab 300 IVD products The Microlab 300 is a hand operated bench top device designed for precision liquid handling The unit is intended for indoor laboratory use by authorized technical staff The unit is also intended for manufacturing purposes with an emphasis on reproducibility tnrough pre prescribed run only methods Conventions Used in this Manual Throughout this manual symbols are used to represent various kinds of information AN WARNING Information that is essential
59. een will display followed by a completely black screen After a few seconds the splash screen will appear again Double click the screen as indicated to enter the calibration program MAAN Tenpi THE MEASURE OF EXCELLENCE TICTOLAB 5 4 4 Screen Power Off The Microlab 300 touchscreen is designed to turn off after an extended period of inactivity The default setting for the power off is 10 minutes If desired the power off time can be increased in the Controller configuration screen 5 4 5 Firmware Software Versions The Microlab 300 is controlled by a combination of three different programs There is firmware on the Syringe Drive Unit which is controlled by software that is running an operating system installed on the Controller The version information for all three of these programs can be viewed in the Software configuration menu within Versions Versions of all Wizards and Linked Methods may also be viewed in this screen 92 CHAPTER 5 CONFIGURATION MENU The most current version of the Microlab 300 firmware and software is posted at www hamiltoncompany com microlab300 under the Technical Information menu This file can be used to update an older Microlab 300 to the current version of firmware and software To update the system download the file and transfer it to the SD card provided with the system Insert the SD card into the side of the controller Then navigate to the Utilities menu and select the Update icon Pre
60. es how quickly the fluid is aspirated Slower soeeds may be used for viscous and or volatile fluids to prevent cavitation and or boiling Speed options include Fast 7 5 pL sec for volumes lt 15 uL 300 uL sec for volumes gt 15 uL Recommended 5 uL sec for volumes lt 15 uL 200 uL sec for volumes gt 15 uL Slow 2 5 uL sec for volumes lt 15 uL 100 uL sec for volumes gt 15 uL Post Aspirate Delay Defines the amount of time the system waits after an aspiration to allow the system to reach mechanical and pressure equilibrium The operator is expected to maintain the tip within the fluid during this time interval Longer delays may be needed to adjust for faster aspirate soeeds and or more viscous fluids 46 CHAPTER 5 CONFIGURATION MENU Dispense Speed Defines how quickly the fluid is dispensed Slower speeds may be used for viscous fluids to allow them to flow to the end of the tip increasing dispense accuracy Speed options include Fast 7 5 pL sec for volumes lt 15 uL 300 uL sec for volumes gt 15 uL Recommended 5 uL sec for volumes lt 15 uL 200 uL sec for volumes gt 15 uL Slow 2 5 uL sec for volumes lt 15 uL 100 uL sec for volumes gt 15 uL Post Dispense Delay Defines the amount of time the system waits after a dispense and before a Subsequent dispense to allow the system to reach mechanical and pressure equilibrium The operator is expected to maintain the tip in a proper dispensing position during this time inte
61. ess the Run button One cycle consists of one complete syringe aspiration and a complete dispense The recommended number of prime cycles is 10 Step 3 Continue priming until there is no air present in the fluid path 28 CHAPTER 2 INSTRUMENT SETUP A Important If air bubbles are still present after several priming cycles verify that all syringe and tubing connections are secure and repeat the priming until the air is purged from the system 2 11 Using the ClickSure Tips The Microlab 300 uses disposable tips for liquid handling The system is optimized using validated ClickSure disposable pipette tips which are available in different sizes purities and with or without a filter The Microlab 300 is only functional if a ClickSure tip has been attached You can either attach the pipette tip by hand or directly insert the end of the probe into a tip held in the tip packaging box If attaching a pipette tip by hand it must be handled in such a way to avoid contamination and heating of the tip Load the ClickSure tip by applying light pressure A tip is properly loaded when an audible click is heard and the O ring sits comfortably within the O ring groove of the ClickSure tip Sr Important To preserve tips for future use the tip package can be resealed by pressing the adhesive lid back onto the tip box 2 12 Installing the Printer Optional The printer upgrade kit that is compatible with the Microlab 300 is part number
62. g valve syringes to remove all air bubbles The system must be primed prior to use and between fluid changes Probe holder This is part of the Cable Management System that holds the Disposable Tip Hand Probe in place Probe receptacle This is the port where the hand probe connects to the Syringe Drive Unit Probe tubing This is the tubing that is installed in the Disposable Tip Hand Probe 116 GLOSSARY Range indicator When putting numerical values for volumes and speeds the pump will have a range indicator depicting the acceptable range for the current setup Reservoir A container that holds the deionized water used to prime the instrument Returned Material Authorization number RMA A number assigned by Hamilton Company which is used to trace an instrument in the event it is returned for repair Reverse pipette A pipetting technique during which a user aspirates more solution that what is to be dispensed Reverse Pipette Wizard This Wizard is used to aspirate slightly more volume than what is defined to ensure an accurate dispense Run screen This screen allows a user to control and monitor all aspects of the instrument s activities Buttons and icons are used to change parameters and indicators are designed to give current and future status of the instrument SD card The SD card is used to store all the methods Liquid Classes and users that are on the pump It is also used to transfer any stored informa
63. id reservoir Step 3 Press the Prime button to begin the prime cycle Step 4 Continue priming the system until the fluid path is clean The number of cycles required to clean the fluid path will depend on the level of contamination present in the system A good estimate is 5 10 cycles of the syringes or 5 10 times the internal volume of the fill and probe tubing Step 5 When the fluid path is clean press the Pause button to stop the priming cycle Step 6 Move the fill tubing from the cleaning fluid reservoir and place it into a reservoir of deionized water Step 7 Direct the hand probe towards a waste container Step 8 Press the Prime button to continue the prime cycle Step 9 Continue priming the system until the fluid path is free of all cleaning fluid Press the Pause button to stop the priming cycle The system is now clean and ready for use 11 5 3 Cleaning the Exterior of the Microlab 300 The Microlab 300 housing is moderately inert to chemical exposure However some chemicals may discolor the surface of the unit If a spill occurs on the exterior of the instrument wipe the surface immediately Wash the surface using a damp cloth with soap and water Then dry the area Make sure liquids do not enter the interior of the Microlab 300 CHAPTER 11 CARE AND MAINTENANCE To disinfect the exterior surface wipe it down with 10 chlorine bleach and deionized water solution Then dry the area Note The cleaning g
64. iew screen will graphically display the progress Note Pressing the Information button at the bottom of each run screen dis plays the pipetting details and parameters being used in the active Wizard A Important If a user makes an error when running a Linked Method pressing the Cancel button on the probe will bring the user to the Linked Method Overview screen The user can select any of the steps in the se quence and continue the method at the desired step If Security is enabled the user will be asked to provide a written justification of the error and reason for cancellation This justification will appear in the log file Step 4 Continue pipetting until the method is complete An audible beep will indicate that the method run is complete At this time if logging is enabled a log file will be generated and stored on the SD card CHAPTER 7 METHOD LINKER 7 4 Editing Deleting Linked Methods To edit or delete a Linked Method select the file that needs to be modified and press the Edit button If the file needs to be deleted select the file and press the Delete button Microlab 300 User Manual 65 CHAPTER 8 UTILITIES CHAPTER 8 This chapter focuses on the Utilities of the instrument In this chapter the following topics will be discussed 8 1 Priming the Instrument 8 2 Moving the Syringes 8 3 View Log Files 8 4 Transferring Error Logs 8 5 Updating the Instrument Firmware and Software Utilities Error L
65. ing and probe cable through the Cable Manager starting after the third tubing clip Weave under each tab as shown in Figure 2 10 Note The Disposable Tip Hand Probe comes with the tubing pre installed Instructions for installing this tubing on the Microlab 300 are located in Section 2 8 Instructions for replacing the tubing are located in Section 11 7 3 WARNING Keep fingers away from hinged openings to prevent pinching of fingers Step 3 Set the Disposable Tip Hand Probe in the Probe Holder as shown in Figure 2 11 CHAPTER 2 INSTRUMENT SETUP Figure 2 11 Assembly of the Disposable Tip Hand Probe Note The entire Cable Management System can be adjusted to the desired position or height of the user Users may also use a standard ring stand rather than the metal rod provided to accommodate the Cable Management System as well as other ring stand accessories Microlab 300 User Manual 25 CHAPTER 2 INSTRUMENT SETUP 2 8 Installing the Fill and Probe Tubing The Microlab 300 Series Pipettor uses 12 gauge tubing for pipetting applications The tubing comes in two different types fill tubing and probe tubing The fill tubing has a blunt end that is designed to go into a reservoir of liquid The probe tubing is preinstalled in the Disposable Tip Hand Probe Instructions for replacing the probe tubing are found in Section 11 7 3
66. iquid Class Wzard 83 11 5 Cleaning ne MENN BOO acicate ati 85 11 5 1 Deciding When to Clean the Microlab 200 85 1 52 _Gleaning the Fluid PAN vassere der 86 11 5 3 Cleaning the Exterior of the Microlab 200 86 11 5 4 Cleaning the Disposable Tip Hand Probe ooccocccocccccncocncncnnnonnn 87 NR Beigl glad Merola 0 6 aseisiin 87 11 7 Replacement Component Installation o cooccoocnnoconocnoncnoncnonnnonoss 88 11 7 1 Valve Hebei 88 11 7 2 Syringe Replacement ccoocccoconoconoconncnonnnonnnonnnoncnoncnonnnnnnononononencnnos 89 11 7 3 Tubing Heplacermment 90 11 7 3 1 Removing Old Probe TUDING soirsicais taras ninia 91 VS Installing New FOSEN annen EA e 91 f ie len EE 92 1 25 O ring FENCE NEE 92 CHAPTER 12 Troubleshooting EE 93 12 1 Troubleshooting Guide 94 12 2 Getting Technical SUpport wi sicsianictnnsnanenieniaceeraviend swhwentevindadennienondaamunin 96 12 3 Returning Instruments for Repalr cccccceeccseeceeecseceeeceeeseeeseeeseess 97 es tele 98 Appendix A Technical SpecificatiONS ooccooccoocioncnoncnoncnoncnonnnornonononononons 98 Appendix B Replacement Parts and Accessories ooccoccconcocnonccocnnaninnonoss 101 Appendix C Chemical Compatibility o ooccooccoocconcconcnoncconcconncononoronnoos 105 Appendix D Instrument Performance Test Reports occcoccccnccccocnnnnnonnnnss 108 NOS e EEE A EEE E E A E 109 WELCOME Welcome Welcome to the World of Hamilton Pr
67. ivity caused by condensation must be expected The data for accuracy and precision only applies after instrument calibration using Hamilton ClickSure pipette tips APPENDICES Table A 2 Microlab 300 Accuracy and Precision Specifications for Deionized Water a Volume uL Accuracy Precision e EEN E e eg E e EE i GE A a SE Se EE a EE EE ORTEN EE o tio hr Se EE EN a EE 8 EN i S SC GE SS EEE i ve A A a A 5 e ae SH is a See eer eee nT i o AA EEE ao see ae ee eee tee 5 ae e ev 5 oo KEE a a eg SE an a EN 1000 0 40 0 15 The data for accuracy and precision only applies after instrument calibration using Hamilton ClickSure pipette tips Microlab 300 User Manual kl 99 APPENDICES Table A 3 Microlab 300 Accuracy and Precision Specifications for Bovine Serum Albumin 87 mg mL Volume uL Accuracy Precision AA A o ea e WEE EE NN ae SE e EE SE GE SE EE z SE Se EE ER a SE o is ee AA a AA a SE e EE SC Ehe SE SE se E ETS SE E gt A SE AA The data for accuracy and precision only applies after instrument calibration using Hamilton ClickSure pipette tips pd Table A 4 Microlab 300 Accuracy and Precision Specifications for Isopropyl Alcohol 99 5 a y Volume uL Accuracy Precision A A GE E i S SE ta e EE EE 8 Se E GE i Ne ae EE GE EE S o GE Se DEE i EE 5 EN EE a A ee ee eee EE S e estate e as eS ee A E 5 BE EE
68. izards for the Microlab 300 including discussions on the following Wizards 6 1 Wizard Basics 6 2 Pipette Wizard 6 3 Reverse Pipette Wizard 6 4 Dilution Wizard 6 5 Aliquot Wizard 6 6 Serial Dispense Wizard Wizards Pipette Reverse Pipette Dilution Y T y Aliquot Serial Dispense 96 CHAPTER 6 WIZARDS 6 1 Wizard Basics To simplify the programming of the Microlab 300 Wizards were created for common pipetting applications These Wizards are designed to enable the user to quickly change from one pipetting technique to the next with minimal time and hassle This section of the manual describes the features that are common to most Wizards 6 1 1 Run 6 1 2 Empty Tip 6 1 3 Favorites 6 1 4 Advanced Settings 6 1 1 Run Run is a small icon button present on every Run screen This button serves a similar purpose to the Accept button on a hand probe When the Run button is pressed the instrument will begin to perform the programmed action To stop immediately press the Back button on the bottom left of the screen or the Cancel button on the hand probe 6 1 2 Empty Tip Empty Tip is a small icon button used to empty the volume within a ClickSure tip after an aspiration This button can be used anytime there is fluid remaining inside the tip The user will be warned that they are going to empty the tip this warning allows the user to properly direct the flow into a reservoir or a waste container 6 1 3 Favorite
69. le e e Scroll Wheel Accept Button e gt e Cancel Button J Figure 4 1 Disposable Tip Hand Probe buttons right handed 34 e Tip Eject Button CHAPTER 4 DISPOSABLE TIP HAND PROBE CONFIGURATION AND OPERATION 4 2 Operating the Disposable Tip Hand Probe The Disposable Tip Hand Probe is held differently than a traditional pipette Reference Figure 4 2 for instructions on how to properly hold the hand probe Figure 4 2 Holding the Disposable Tip Hand Probe Accept button Located next to the thumb used to activate pipetting functions and to choose OK from pop up screens Cancel button Located on the opposite side of the Accept button used to activate Empty Tip function or to choose Cancel from pop up screens Scroll button Located top center of the probe used to access method Favorites and to select items from a list box Microlab 300 User Manual 35 CHAPTER 4 DISPOSABLE TIP HAND PROBE CONFIGURATION AND OPERATION 36 Tip Eject button Located near the index finger used to eject ClickSure tip Note For tips on cleaning the Disposable Tip Hand Probe reference Section 11 5 4 Biohazard When using the Microlab 300 always observe Good Laboratory Practices GLP Wear suitable protective clothing safety glasses and protective gloves particularly when handling biohazardous samples and when dealing with spilled liquids and contaminated areas of the instrument
70. nge drive with a thumbscrew The syringe drive moves up and down to dispense and aspirate fluid accordingly 2 2 2 Description of the Rear View of the Syringe Drive Unit MICrOLAB C e ISO 8655 2 Power On Off Switch gt gt O Probe Receptacle E Accessory Receptacle 6 pin mini DIN HAMILTON Controller Receptacle 6 pin mini DIN HAMILT2N PN 66260 01 REV X1 SN ML300XX00001 KN e Figure 2 2 Accessory Receptacle and Controller Receptacle o VDC Receptacle Power on off I O Switch The Power on off switch is located in the upper right corner on the back of the Syringe Drive Unit The switch toggles on or off I on O off Probe Receptacles There are two probe receptacles located on the back of the instrument under the Power switch The Disposable Tip Hand Probe may be inserted into either receptacle When a signal is received by the Syringe Drive Unit through the probe receptacle the syringe drive moves to perform the next action in the current method 18 CHAPTER 2 INSTRUMENT SETUP Accessory Receptacle 6 pin mini DIN This port will be used for future expansion of the system Controller Receptacle 6 pin mini DIN The Controller receptacle is located in the lower right corner on the back of the Syringe Drive Unit This port is used to connect the Controller to the Syringe Drive Unit using the grey Controller cable VDC Receptacle
71. nsing Number of pre wet cycles The number of aspiration and dispense cycles that will occur prior to aspiration of the desired volume to wet the inside of the ClickSure tip Number of replicates Defines the number of times a particular volume will be pipetted and weighed during gravimetric calibration The software will calculate the average mass from these replicates and use this average mass value and the density to adjust the data points of a Liquid Class curve Numeric keypad A group of keys on the Controller display that are used to enter numeric data 114 GLOSSARY OTG USB Port The OTG USB port is located on the back of the Controller This port is used for diagnostics and testing at Hamilton Company prior to shipment Parameter adjustment buttons These buttons show the current dispense volume and tip settings The exact function for these buttons will be defined in greater detail in the Wizards and Linked Method sections of the manual Parameter indicator This indicator shows the value of a parameter but does not allow for the adjustment of the parameter Pipette To measure a fixed amount of liquid with a probe and then dispense that same volume of liquid into a vessel Pipette Wizard A Wizard designed to allow the user to program a pipetting application Power cycle To turn the Microlab 300 off and then back on Power down To turn an electrical device off Power on off switch The switch on the back of the
72. o either side of the Microlab 300 that holds the Disposable Tip Hand Probe See Section 2 6 on functionality and assembly of the Cable Management System Cable Manager This component connects to the Accessory Holder of the Cable Management System and helps hold the probe cables and tubing off of the lab bench See section 2 6 on functionality and assembly of the Cable Manager Cancel button This button is located on the Disposable Tip Hand Probe and is designed to stop the syringe drive unit from performing an application It is also used to cancel popup screens or to enable the Empty Tip functionality Change buttons These are buttons that are used to edit fields such as date and time in the different screens ClickSure tips These are the tips that are validated for the Microlab 300 Series Pipettor and are compatible with the Disposable Tip Hand Probe ClickSure Tip Volume Indicator This indicator shows the current volume of solution in the ClickSure Tip Configuration screen This is the screen on the Controller where the user can set Liquid Class Hardware settings Software settings Security Settings Log Settings and User accounts for the unit Controller receptacle This is the receptacle where the grey 6 pin Mini DIN Controller cable connects from the Controller to the Syringe Drive Unit 110 GLOSSARY Controller unit This component allows the user to send operating instructions to the Syringe Drive Unit Cycle
73. o update the Microlab 300 software and firmware to the version that is present on the SD Card Main Menu Small Icons Favorites Press to recall all applications that are frequently used and saved as a Favorite 14 CHAPTER 10 ICON INDEX Wizards Small Icon Buttons Run Press to trigger the instrument This icon functions identically to the Disposable Tip Hand Probe Accept button Favorites Press to recall applications within the active Wizard that are frequently used and saved as a Favorite Advanced Press to see the advanced detail of the current Wizard See Section 6 1 4 for more details Default Press to change settings back to their Hamilton default values Up Press to move a step in the Serial Dispense Editor upward in the sequence Microlab 300 User Manual 75 CHAPTER 10 ICON INDEX 76 Function Duplicate Press to copy a step in the Serial Dispense Editor The step will be added directly below the step that was copied Add Step Press to add a new step in the Serial Dispense Editor This new step will be added to the bottom of the sequence Linked Method Small Icon Buttons Run Press to trigger the instrument This icon functions identically to the Disposable Tip Hand Probe Accept button History Press to view the edit history of a Linked Method e CHAPTER 10 ICON INDEX Function Mix Press to add a mix step in a Linked Method
74. ocncccnoncnoncnoncnoncnonononononononenonenonoss 52 5 4 6 SD Card Capacity and System Memory ooccoccncncnnncinncnnncnonnnonononnos 59 547 o A 59 548 System Calbraton Date susana iia 53 D NSM 53 5 6 LOO OONO EEE EE 54 STU Ted 54 5 7 1 Add Ueer nrn nern r nrnna nrnna errr n errn errr r nna 59 5 7 2 Edit USE A A 55 KE Goes CWI erg USOS rs sonaron EENE ero DO ORAP IER E e CC 56 ol EN 57 A e e UE O ines D A EE EAE E A E St ol Ne Sr 6 1 4 Advanced Settings occccccccccnconcnccononocnncnnnononooncnonononncnononnonanennnnns 56 ben NG 58 6 3 Reverse Pipette Wizard A e 0 UC 59 O NNN 59 ERAN OU e EE 59 6 6 Serial Dispense Ward 60 CHAPTER 7 Method J EE 61 ke OF Linked MSI JE 62 7 2 roang Wee WR ee 62 73 FUNDING LINKS Ne e 63 7 4 Editing Deleting Linked Methods A 65 EE C UNS aras 66 8 1 Priming the Instrument 67 8 2 MOVING the ln e 67 8 3 WS IN Log EN n a i aS 67 ios nn 67 8 5 Updating the Instrument Firmware and Software essersreerrerrerrerren 68 CHAPTER 9 Best Pipetting Drachce 69 9 1 Preparing to Heite 70 Gees 910 1 6 e EE 70 9 3 Dispensing e IER 70 9 4 Pipetting Highly VISCOUS Solutions Luren seeren 71 Sr TER 10 COR NC EE 72 CHAPTER 11 Care and MANENANGE msictonmartamaei ia 80 VIL VTS SN 11 2 Weekly Matenance 81 11 3 Yearly Maintenance rrrarrnrrrrrnnrrrarnannrerrarerarsasrnsrrsnnnersasnnnersrsnsersesnnnn SN 11 4 Instrument Calibration 81 114 1 NN 82 11 4 2 Calibration using the L
75. og Update 66 CHAPTER 8 UTILITIES 8 1 Priming the Instrument This section describes how to properly prime the Microlab 300 before beginning to pipette From the Main Menu on the Controller screen select the Prime button Select the appropriate number of cycles and press the Run button One cycle consists of one complete syringe aspiration and a complete dispense The recommended number of prime cycles is 10 If there is still air in the fluid path after the priming verify that all tubing and syringe connections are tight and repeat the priming until all air bubbles have been removed from the system 8 2 Moving the Syringes To remove or install new syringes on the Microlab 300 Series Pipettor the user must first lower the syringe drive To raise or lower the syringes press the Utilities button from the Main Menu and then select the Move Syringes button There will be two buttons on this page one for raising the syringes and one for lowering the syringes 8 3 Viewing Log Files In the Log Viewer the user may view log files for methods that have been executed on the instrument or print the log file directly from the Controller when connected to the printer upgrade kit p n 61500 04 The log file shows the details of the method run including number of loops tip size tip type mix mode cancellations sample volume Liquid Class and pre wet mode The user may also use the LyncStore online service provided by Hamilton to store
76. ost Dispense Delay Post Dispense Audible and Number of Pre wet Cycles For details on these parameters reference Section 5 2 1 Note If pipetting over the complete volume range of the Microlab 300 using a single tip type it will be necessary to create two Liquid Classes one for the 50 uL tip and one for the 1 mL tip Step 7 Once all parameter values have been entered select the Data Points button in the lower right part of the screen All data points will be pre populated from the duplication of the original curve Step 8 Add or delete volume points using the icons at the bottom of the screen 48 CHAPTER 5 CONFIGURATION MENU Note In the data points screen there will be a high and low volume point for each tip size that cannot be modified For example the 50 uL tip has a high point of 50 uL and a low point of 1 uL When creating the calibration curve it is recommended that these high and low volume points be used Step 9 Now the system can be gravimetrically calibrated Refer to Section 11 4 for instrument calibration 5 2 4 Editing Deleting Liquid Classes To edit an existing Liquid Class the file must be selected from the Liquid Classes Edit Classes screen The user may edit the Liquid Class accordingly by following the instructions in Section 5 2 3 beginning at Step 4 To delete a file the user may select the file from the Liquid Classes Edit Classes screen and press the Delete button Note The standard Liqui
77. pose of gloves as biohazardous waste Do not put your hands or face near the syringe drive mechanism while the instrument is running 14 CHAPTER 2 INSTRUMENT SETUP CHAPTER 2 Instrument Setup This chapter provides detailed information on the installation of the Microlab 300 hardware including 2 1 Overview of Microlab 300 Parts List 2 2 Describing Instrument Components 2 3 Selecting the Proper Location 2 4 Charging the Controller 2 5 Installing the Controller and Syringe Drive Unit 2 6 Installing the Cable Management System 2 7 Installing the Disposable Tip Hand Probe 2 8 Installing the Fill and Probe Tubing 2 9 Applying Power to the Instrument 2 10 Priming the Instrument 2 11 Using the ClickSure Tips 2 12 Installing the Printer Optional Microlab 300 User Manual 15 CHAPTER 2 INSTRUMENT SETUP 2 1 Overview of Microlab 300 Parts List The Microlab 300 instrument comes with everything needed for installation For replacement parts please refer to Appendix B Replacement Parts and Accessories Carefully unpack the Microlab 300 components and verify that all parts have been received The parts list for the instrument is displayed in the table below Table 2 1 Microlab 300 Parts List Component Part Number Quantity Microlab 300 User Manual 67258 01 1 Hardware Quick Start Guide 67273 01 1 Software Quick Reference Card 67274 01 1 Syringe Drive Unit Includes valve 1 25 mL syringe 66260 01 1 and 25 uL s
78. r elevation because the data collected and entered into the software was gathered in real time and not at the factory Elevation compensation will only be used for the three standard Liquid Classes shipped with the system 5 4 3 Calibrating the Controller Touchscreen The controller touchscreen is calibrated before it leaves Hamilton Company If the clicked area on the touchscreen does not seem to align with the image on the screen It is probably time to recalibrate the screen Step 1 To reset the touchscreen calibration select the Software icon from the Configuration screen Next select the Controller View button and scroll down to the Screen Calibration option and press Calibrate The following screen should appear Carefully press and briefly hold stylus on the center of the target Repeat as the target moves around the screen Press the Esc key to cancel Step 2 Press the center of the cross Step 3 The cross will move to different locations on the screen Each time the cross stops moving press the center Step 4 Once all 5 positions have been recorded press anywhere on the screen to store the calibration values Microlab 300 User Manual 51 CHAPTER 5 CONFIGURATION MENU If the calibration is so far off that it is impossible to navigate to Screen Calibration as described in Step 1 it is possible to get to the calibration program during system start up Turn the instrument Off and then On The initial solash scr
79. rial Dispense Wizard will move to the next dispense Discrete The Discrete functionality in the Serial Dispense Wizard allows the user to aspirate and dispense each volume in the Edit Steps one at a time rather than aspirating the total volume of dispenses The Discrete functionality can be enabled within the Advanced button of the Serial Dispense Wizard 60 CHAPTER 7 METHOD LINKER CHAPTER 7 Method Linker This chapter provides details regarding Linked Methods and includes a discussion of the following topics 7 1 Overview of Linked Methods 7 2 Creating Linked Methods 7 3 Running Linked Methods 7 4 Editing Deleting Linked Methods Microlab 300 User Manual 61 CHAPTER 7 METHOD LINKER 7 1 Overview of Linked Methods Linked Methods are useful when a user wants to complete an entire pipetting application from start to finish without having to access each Wizard separately A Linked Method is a sequence of pipetting Wizards and or Favorites strung together Linked Method Editor VEN Aliquot Reverse Pipette Dilution Loop 1 Liquid Class Aqueous300Default Sample Volume 1000 lip Type Standard lip Size 1 mi Pre Wet Mode Off Mix Mode Off a Method Selector UI Forward Edit Delete Linked Methods serve as electronic SOPs and produce one log file upon completion All parameters of each Wizard or Favorite can be modified during the creation of a Linked Method Instructional prompts can also be
80. rval Longer delays may be needed to adjust for faster dispense speeds Post Dispense Audible If enabled an audible buzzer will indicate that a dispense is complete Number of Pre wet Cycles The number of aspiration and dispense cycles that will occur prior to aspiration of the desired volume to wet the inside of the ClickSure tip The tip type and size used to create a Liquid Class are also important Liquid Classes must be defined for each of the ClickSure tip sizes and types The user must select which tip size and type non sterile sterile or sterile filtered that is to be used and create a Liquid Class For example if a user uses only sterile filtered tips but uses volumes across the volume range of 0 5 to 1 000 uL the user must create two Liquid Classes one for each tip size The Microlab 300 SD card can store hundreds of Liquid Class files For archiving purposes Hamilton has provided a PC application available online called LyncStore This application allows for management and archiving of Liquid Class files transferred from the SD card to a PC From this PC application you can filter archive and view Liquid Class files Learn more about the LyncStore at www hamiltoncompany com Microlab 300 User Manual 47 CHAPTER 5 CONFIGURATION MENU 5 2 2 Standard Liquid Classes The Microlab 300 offers three standard Liquid Classes for the user Default300Aqueous Developed using deionized water per ISO 8655 standards Default300
81. s Operations that are performed frequently can be stored as Favorites and then recalled for use at a later time Press the Favorites button to store the current Wizard setup as a Favorite or to change the current Wizard setup to a previously stored Favorite setting When selecting a previously stored Favorite the menu will display only the Favorites that were stored for the given Wizard being executed Microlab 300 User Manual 57 CHAPTER 6 WIZARDS 6 1 4 Advanced Settings D For most applications the default settings in each Wizard will provide the best results For applications that require greater control over the instrument the Advanced menu provides an interface to adjust the following settings Liquid Classes are designed to help the user compensate varying fluid properties By compensating for different liquid properties the dispenses from the instrument will be more accurate and precise The instrument comes with three standard Liquid Classes Users may also create custom Liquid Classes See Section 5 2 3 for more details Pre wet Pipette and Dilution Wizards only Pre wet is the optional process of aspirating and dispensing sample to coat the inside of a pipette tip prior to aspirating a defined sample volume The pre wet function helos ensure accuracy of viscous liquids by decreasing the cohesive forces between tip and sample Pre wet also helps with volatile fluids as evaporation within the tip can cau
82. samples observe and perform the maintenance procedures with a focus on cleaning and decontamination Wear gloves when handling the instrument and components Avoid touching tips discarded into waste container Any surfaces on which liquid is spilled must be decontaminated If working with biohazardous or chemical materials the user must not touch them Biohazard Material used with the Microlab 300 may be considered biohazardous Dispose of hazardous or biologically contaminated materials according to the practices of your organization Discard all materials in a safe and acceptable manner and in compliance with all federal local regulatory and jurisdictional requirements Always observe Good Laboratory Practices GLP Wear suitable protective clothing safety glass and protective gloves particularly when handling biohazardous samples and when dealing with spilled liquids and contaminated areas of the instrument Avoid touching tips discarded into the laboratory supplied waste bin Any surfaces on which liquid is spilled must be decontaminated Microlab 300 User Manual 13 CHAPTER 1 INTRODUCTION 1 2 5 Protective Equipment It is the user s responsibility to know and understand the level of protective equipment that must be worn when working with radioactive biohazardous and harsh chemicals When using the Microlab 300 wear personal protective apparel including disposable gloves Thoroughly wash hands after removing gloves and dis
83. se a significant loss of sample before delivery Pre wet increases the humidity within the tip thus reducing both the amount of variation and sample evaporation It is recommended to use this setting for volatile or viscous fluids to improve pipetting accuracy If enabled the ClickSure tip will be pre wetted three times prior to the sample aspiration Mix Pipette and Dilution Wizards only This setting allows a user to mix after performing a pipetting or dilution step The user may select the volume to mix If enabled pressing the Accept button will begin the repeated aspiration and dispense cycle Pressing the Accept button again will end the mixing cycle 6 2 Pipette Wizard 08 This Wizard is used to aspirate and dispense single samples Once the system is primed pressing the Accept button causes the defined amount of sample to be aspirated into the ClickSure tip A second press of the Accept button causes the liquid to be dispensed from the ClickSure tip into the destination vessel CHAPTER 6 WIZARDS 6 3 Reverse Pipette Wizard This Wizard is used to aspirate and dispense single samples During a Reverse Pipette the system will aspirate slightly more volume than what is defined Once the system Is primed pressing the Accept button causes the defined amount of sample to be aspirated into the ClickSure tip plus an additional amount of sample 5 uL when using a 50 pL tip and 25 uL when using a 1 mL tip A second press
84. sists of a User ID First Name Last Name enabled or disabled flag user key and user roles The user roles define what Controller settings a specific user IS able to change Only one account can be set to have Administrator access All other users can have at least one of the following roles be able to operate the Wizards or Linked Methods in a run only mode be able to modify probe handedness be able to manage user accounts or be able to program Wizards or Linked Methods In addition to editing account information the Edit User screen allows for the deletion of a user or to reset a user key in the event it is forgotten 5 7 3 Export and Import Users To export users simply press the Export Users button and the data will be saved on the SD card To import user accounts onto your Controller insert the SD card that contains the desired user files and press the Import Users button The user accounts will be uploaded from the SD card onto the new Controller The Microlab 300 SD card can store many user accounts For archiving purposes Hamilton has provided a PC application available online called LyncStore This application allows for management and archiving of files transferred from the SD card to a PC From this PC application you can filter archive and view user accounts Learn more about the LyncStore at www hamiltoncompany com Microlab 300 User Manual 55 CHAPTER 6 WIZARDS CHAPTER 6 Wizards This section discusses the W
85. ss start to begin the update and follow on screen instructions to complete the update 5 4 6 SD Card Capacity and System Memory In the Controller configuration screen there are indicators showing percent memory used on the SD card and the system memory 5 4 7 System Trace Logging If the Microlab 300 is having technical difficulties Hamilton Technical Support staff may require more detailed system trace logs to troubleshoot the problem This can be enabled within the software configuration menu in the Controller screen Simply select System Trace Logging and turn this feature on The system will automatically log additional data from the software application and report this information in the Error log refer to Section 8 4 for discussion of Error Logs Note The System Trace Logging will be reset to Off if the instrument is powered off 5 4 8 System Calibration Date After a system has been calibrated the calibration date can be stored 5 5 Security Settings For laboratories requiring traceability the Microlab 300 provides the option for users to log in before they can create edit or run methods To enable the use of passwords and user accounts select Security Settings This screen manages the use of user accounts the expiration of the user keys and the maximum Microlab 300 User Manual 53 CHAPTER 5 CONFIGURATION MENU number of times an incorrect user key can be entered before the user account is locked The first time
86. th requires health maintenance to prevent clogs which would prevent device from performing per calibration It is recommended to change the deionized water in the fill reservoir once a week to avoid contaminants which may cause clogs in the instrument s fluid path and thus prevent the instrument from performing per calibration 11 3 Yearly Maintenance It is recommended that the Microlab 300 be sent to a service center for cleaning and lubrication on an annual basis Upon receiving the instrument a Hamilton technician will evaluate the instrument to make sure it is in good working order The belts will be replaced and the lead screw and shaft will be cleaned and replaced if necessary The lead screw and shaft will be lubricated Finally the instrument will be retested and recertified for calibration 11 4 Instrument Calibration Many companies require that instrumentation including the Microlab 300 be validated on a monthly semi annual or annual basis The instrument can be sent to Hamilton Company for recertification at any time Simply contact Hamilton Customer Service to obtain a Return Materials Authorization RMA number Note Sending the Microlab 300 in for non warranty service will be charged to the customer Hamilton s validation procedure for accuracy and precision are performed according to the ISO 8655 standard There are instructions on validating the Microlab 300 on our website at www hamiltoncompany com microlab300 Mi
87. ther the set aspiration and dispensing speeds as well as pipetting delays are suitable for the liquid These parameters can be viewed within the Liquid Class screen Choose from either the standard Liquid Classes or create a new Liquid Class as described in Section 5 2 3 Be sure that the chosen Liquid Class has been validated for the pipetting method and liquid being used 9 2 Aspirating Liquid Step 1 Step 2 Step 3 Step 4 Step 5 Step 6 Pre wet the new ClickSure tip by aspirating and dispensing the liquid to be pipetted one to three times Immerse the ClickSure tip into the liquid vertically approximately 3 mm deep Immersing the tip too deep in the liquid will have adverse effects while immersing the tip too shallow may result in air bubbles Press the Accept button on the probe to aspirate liquid Maintain the immersion depth so that no air is aspirated accidentally After aspiration keep the tip in the liquid for approximately 2 seconds particularly for viscous liquids After aspiration pull the tip slowly out of the liquid Wipe the tip against the container wall to remove any liquid which may be present on the outside of the ClickSure tip 9 3 Dispensing Liquid Step 1 When dispensing liquid place the ClickSure tip at a slight angle against the wall of the 70 Step 2 Step 3 Step 4 Step 5 well or tube Press the Accept button on the probe to dispense the liquid Wait until no more liquid is
88. tight connection between the valve and tubing Step 6 Re thread the tubing and probe cable into the Cable Manager by weaving under each tab Plug the probe cable into one of the two probe receptacles on the instrument 11 7 4 Battery Replacement In the Controller there is a battery to maintain the date and time in the event that power Is lost to the instrument Should the battery fail the Controller will need to be sent in for service The battery should have a life of 10 or more years 11 7 5 O ring Replacement Step 1 Gently grasp and hold the ejection sleeve up on the probe Step 2 Use forceps or tweezers to remove the old O ring from the groove on the probe Step 3 Discard the old O ring Step 4 Gently thread the replacement O ring onto the end of the probe and into the groove 92 CHAPTER 12 TROUBLESHOOTING CHAPTER 12 Troubleshooting This chapter includes information on troubleshooting the Microlab 300 which includes the following topics 12 1 Troubleshooting Guide 12 2 Getting Technical Support 12 3 Returning Instruments for Repair Microlab 300 User Manual 93 CHAPTER 12 TROUBLESHOOTING 12 1 Troubleshooting Guide This section contains information on troubleshooting the Microlab 300 instrument Table 12 1 Troubleshooting Guide Problem Possible Causes Corrective Action Disconnected power cord or Connect power cord and check Instrument does not faulty power outlet power Source power
89. tion to another Controller or to a PC Serial dispense During a serial dispense a user repeatedly dispenses aliquots of differing volumes at the press of a button The total volume is drawn into the tip and then multiple dispenses are performed as specified Serial Dispense Wizard This Wizard is used to program a serial dispense using the instrument Microlab 300 User Manual 417 GLOSSARY Shift button This button toggles letters from lowercase to uppercase Small icon buttons These buttons provide additional functionality to change values on the Current screen Speed See Aspirate soeed and Dispense speed Syringe drive mechanism The arms to which the syringe plungers connect The movement of the syringe drive arms moves the syringe plungers which displaces fluid drawing it through the system Syringe Drive Unit The heart of the Microlab 300 that contains the valves syringes tubing and the syringe drive mechanism Syringe initialization Driving the syringe to the top of stroke towards the valve System fluid The fluid originating from a reservoir that is pumped through the entire system during priming The recommended system fluid for the Microlab 300 is deionized water Timer The timer is a function in the Linked Method screen that allows the user to insert a time delay after a given method step This is most commonly used for incubation times Tip eject button This button on the Disposable Tip Hand Prob
90. ts for a class B computing device pursuant to FCC Rules In order to maintain compliance with the FCC regulations shielded cables must be used with this equipment Operation with non approved equipment or unshielded cables is likely to result in interference to radio and TV reception The user is cautioned that changes and modifications made to the equipment without the approval of the manufacturer could void the user s authority to operate this equipment Microlab 300 User Manual Table of Contents KEIER NOC ge de D 2 EEE EN 3 Table of Contents EE 4 WV CNG ONS ENE EEE 8 Mee O enn ae ee eee EEE 9 TFT 10 1 1 Introduction of the Microlab 300 Series Pipettor cooccocccocncocnconnnnoo 11 Mee VM GE 11 1 2 1 General Safety Information occcocioocconcnonnnonnnonononoconoconnnnonononononnos 11 1 2 2 Operating the Microlab 300 Series Pipettor ocoocccocncccncncnnnonononos 12 A o e E A ee re ane 12 A A An 18 EE EE 14 CHAPTER 2 Instrument SOUP sino 15 2 1 Overview of Microlab 300 Parts Let 16 2 2 Describing Instrument Components coocccccnccncnnncnnnncnnnnonenonnonnnoncnnnninnnos 17 2 2 1 Description of the Front View of the Syringe Drive Un ie 2 2 2 Description of the Rear View of the Syringe Drive Um 18 2 2 3 Description of the Rear View of the CGonmtroler 19 2 3 Selecta the Proper LOCGANON ER 20 241 ONG me SONE er l 20 2 5 Installing the Controller and Syringe Drive Um 21 2 6 Installing the Cable
91. ual 127 2012 Hamilton Company All rights reserved Corporation in the United States and other countries Microsoft ea C and Visual Basic are regi l and Marathon Paper Mills Company All rights reserved Parafilm is a registered trademark of Pe Labi isa trademark Oi Nati d al Instru ny nor any software programs or other goods or services d with endorsed by or sponsored by National Instruments red by Hamilton Company in the U S and or other countries Part No 67258 01 11 2012 4 Hamilton Americas amp Pacific Rim Hamilton Europe Asia amp Africa 4970 Energy Way Via Crusch 8 Reno Nevada 89502 USA CH 7402 Bonaduz GR Switzerland Tel 1 775 858 3000 Tel 41 81 660 60 60 Web www hamiltoncompany com Fax 1 775 856 7259 Fax 41 81 660 60 70 e1010 D 4 8 59 5O salesQhamiltoncompany com contactOhamilton ch USA Europe 41 8 1 660 60 60 To find a representative in your area please visit hamiltoncompany com contacts
92. uidelines presented here are meant as guide only If applications require different cleaning solutions contact Hamilton Company for more information 11 5 4 Cleaning the Disposable Tip Hand Probe Clean the probe with an alcohol such as methanol or ethanol Do not soak the probe or use acetone to clean as acetone may dissolve the plastic housing of the probe The Disposable Tip Hand Probe is not autoclavable Note The hand probe s fluid path is manufactured from FEP tubing that is chemically inert For chemical compatibility please reference the Microlab manual Appendix C Biohazard When using the Microlab 300 always observe Good Laboratory Practices GLP Wear suitable protective clothing safety glass and protective gloves particularly when handling biohazardous samples and when dealing with spilled liquids and contaminated areas of the instrument Avoid touching tips discarded into the laboratory supplied waste bin Any surfaces on which liquid is spilled must be decontaminated 11 6 Storing the Microlab 300 For long term storage prime and purge the system with ethanol to facilitate drying Remove the tubing and syringes Cover the instrument to protect it from damage Microlab 300 User Manual 87 CHAPTER 11 CARE AND MAINTENANCE 11 7 Replacement Component Installation This section contains instructions for replacing the components on the Microlab 300 including the following 11 7 1 Valve Replacement 11 7 2 Syringe
93. use leaks or damage to the valve Syringes that are under tightened may cause leaks WARNING Use extreme caution when removing cracked or broken syringes Always wear protective gloves and eye wear 11 7 3 Tubing Replacement The Microlab 300 Pipettor has two tubing assemblies There is a fill tubing assembly that should be attached to the left port of the valve To replace this tubing simply unscrew the existing tubing assembly and discard Follow the directions in Section 2 8 on installing the tubing The probe tubing is integrated into the Disposable Tip Hand Probe and attaches to the right port of the valve Follow the directions in Section 2 8 on installing the probe tubing on the system To replace the probe tubing follow the instructions below To replace the tubing on the Disposable Tip Hand Probe the existing tubing needs to be removed first 90 CHAPTER 11 CARE AND MAINTENANCE 11 7 3 1 Removing Old Probe Tubing Step 1 Unscrew the tube fitting from the valve Unplug the probe cable from the instrument Remove the tubing and probe cable from the Cable Manager Step 2 Unscrew the probe tip and discard Step 3 Cut off the tubing end with the ferrule and stainless steel ring and unthread the fitting Step 4 Begin by pushing the tube out through the tip of the hand probe Then pull the remaining tubing out and discard Probe Cable Tubing Clip eien G ord O OOOO 1 4 28 Fitting Stainless Steel Ring
94. user accounts are enabled the system will automatically create an Administrator account User ID Admin and User Key 7913 Once user Accounts are enabled the system will force the user to log in as the Administrator before any other settings can be adjusted 5 6 Log Settings The Microlab 300 Controller provides the ability to save a log of pipetting activities to the SD card Logging can be enabled per Wizard or Linked Method For regulated customers requiring 21 CFR Part 11 compliance Electronic Signatures can be enabled if the Security Settings are also enabled Once Electronic Signatures are enabled all Wizards and Linked Methods must be logged If multiple systems are used by the same laboratory a unique Controller name can be entered to simplify the management of log files The Controller name can be set within the Log Settings Configuration menu Log files created on the Microlab 300 can be viewed and printed directly from the Controller by purchasing the printer upgrade kit p n 61500 04 For more details on the printer option refer to Section 2 12 The Microlab 300 SD card can store hundreds of log files depending on the number of activities recorded in each log For archiving purposes Hamilton has provided a PC application available online called LyncStore This application allows for management and archiving of files transferred from the SD card to a PC From this PC application you can filter archive view and print log files
95. ws the direction of fluid movement in or out of the ClickSure tip in the next step 5 1 3 Data Entry Screens Data Entry screens allow the inputting of values for several parameters The values that can be changed are displayed in a table format Editing a value is enabled when the selected row is highlighted Serial Dispense 40 CHAPTER 5 CONFIGURATION MENU 5 1 4 Pop up Control The Pop up Control appears as a modal window within the current screen All buttons outside of the modal window will be disabled until the OK button is pressed to close the modal Serial Dispense r Edit Step Tip gt I17e There is no liquid aspirated to empty 5 1 5 Pick List The Pick List screen allows the user to select from a list of options Options can include a choice of settings or applications to activate All buttons outside of the Pick List screen remain disabled until the Accept or Cancel button is pressed to close the screen Microlab 300 User Manual 41 CHAPTER 5 CONFIGURATION MENU Linked Method Editor Select Wizard To Add DI Ali Pipette si Aliquot Loop 1 Reverse Pipette Timer Perio Dilution Accept Cancel a Method Selector Y A P Back Forward Edit Delete 5 1 6 Linked Method Screens There are two main screens in the Linked Method The first screen is the Linked Method Editor This screen allows the user to add and delete different Wizards and steps in a Linked Method
96. xample Calibration Certificate GLOSSARY Glossary Accept button This button is located on the Disposable Tip Hand Probe and is designed to trigger the syringe drive unit to perform the next step in an application It is also used to select OK in popup screens or to enable the Run functionality Accessory Holder This component is part of the Cable Management System and connects directly to either side of the Syringe Drive Unit to support the Probe Holder and Cable Manager See Section 2 6 on functionality and assembly of the Accessory Holder Accessory receptacle This is a port on the Syringe Drive Unit that will be used for future expansion of the Microlab 300 Series Pipettor Aspirate To take in a defined volume of liquid into a ClickSure tip on the Disposable Tip Hand Probe Aspirate speed Defines how quickly the fluid is aspirated Slower soeeds may be used for viscous and or volatile fluids to prevent cavitation and or boiling Aliquot Wizard A Wizard in which fluid is distributed multiple times in equal volume increments Back button This button will take the user back to the previous screen Blowout Removing excess fluid remaining in the tip using air in the system Button mapping This function will allow the user to change the handedness on the Disposable Tip Hand Probe to either left or right handed Microlab 300 User Manual 109 GLOSSARY Cable Management System This is the component that attaches t
97. yringe pre installed Controller Includes Controller cable and SD card SEET 1 Disposable Tip Hand Probe EEE Includes instruction sheet Cable Management System Includes instruction sheet Accessory Holder 65160 01 1 Cable Manager and Probe Holder 12 gauge fill tubing 61614 01 1 Power supply 61092 01 1 Reference Power cord Aopen 1 This is a country specific power cord Reference Appendix B for the appropriate part number 16 CHAPTER 2 INSTRUMENT SETUP 2 2 Describing Instrument Components This section shows detailed diagrams of the front and back of the Microlab 300 Syringe Drive Unit and Controller It also provides a description of the buttons and port receptacles required to install and operate the Microlab 300 Pipettor as described in following chapters 2 2 1 Description of the Front View of the Syringe Drive Unit Valve Syringe Drive LA Figure 2 1 Front View of the Microlab 300 Syringe Drive Unit Valve Actuator The valve actuator turns the valve automatically to aspirate and dispense liquids Valve direction is dependent on the volume being aspirated or dispensed Microlab 300 User Manual 47 CHAPTER 2 INSTRUMENT SETUP Syringe Drive The syringe drive mechanism positions the Hamilton precision syringes with the high resolution stepper motor The syringes are threaded into the valve and the plunger is attached to the syri

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