Order 800.453.0385 - Denville Scientific Inc.
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1. To exclude an area from counting tap on Manual on the top menu and then tap on Exclusion Zone Then tap around the region you want to exclude four sepa rate taps The highlighted exclusion zone area will then appear To remove an existing exclusion zone double tap inside of the zone Reset Image review Exclusion Zone Camera Controls Dilution Calculator Help Manual Add Live Cells Add Dead Cells Exclusion Zone Set Cell Size WWwW densci com e info densci com e Fax 508 429 5732 i19 800 453 0385 84 October Hill Road Holliston MA 01746 17 DENVILLE SCIENTIFIC INC a division of Harvard Bioscience Inc Adjusting the Count Sensitivity To increase the counting sensitivity slide blue vertical sensitivity bar located be tween the image and notes section up To decrease the counting sensitivity slide blue vertical sensitivity bar down ihe 800 453 0385 www densci com info densci com Fax 508 429 5732 84 October Hill Road Holliston MA 01746 18 DENVILLE SCIENTIFIC INC a division of Harvard Bioscience Inc Adjusting Cell Size Parameters Tap on the Manual tab at the top and then on Set Cell Size option You will then see the sliding Max Min scale to the right side of the screen To increase the minimum cell size slide finger up To decrease the minimum cell size slide finger down Min Size M www densci com info densci com e Fax 508 429 5732 i19 800 453 0385 842. DENVILLE SCIENTIFIC INC a division of Harvard Bioscience Inc 100 240 VAC 60 HZ 1 5 amps 2 5 Ib shipping weight 5 Ib 6 5 inches tall x 4 5 inches diameter 5 to 60 um recommended diameter range Trypan Blue 10 wl x 1045 x 1047 cells mL Compatible with Biorad Luna and Countess slides Standard microscope slides with slip covers may be used for preliminary testing Android based operating system Compatible with USB flash drive UL approved LED lights power supply amp camera Compatible with FDA 21 CFR part 1 export as PDF 2 year repair or replacement For Research Use Only Not for any human therapeutic or diagnostic use www densci com info densci com Fax 508 429 5732 84 October Hill Road Holliston MA 01746 DENVILLE SCIENTIFIC INC a division of Harvard Bioscience Inc Hardware Installation Install hardware as shown in diagram below For RS RC 150 only RevCount I 120AC USB Installation diagram micro USB Tablet CPU micro USB USB USB Belkin hub USB flash drive ports flash drives not included RevCount WWwW densci com info densci com e Fax 508 429 5732 i19 800 453 0385 84 October Hill Road Holliston MA 01746 DENVILLE SCIENTIFIC INC a division of Harvard Bioscience Inc START COUNTING From out of the box to counting colonies Quick Setup device as seen on page 9 and turn on 2 Adjust li
3. i19 800 453 0385 84 October Hill Road Holliston MA 01746 DENVILLE SCIENTIFIC INC a division of Harvard Bioscience Inc Safeguards amp Cautions To maximize the use of the RevCount please follow the recommendations in this short list before using Examine carton and contents for possible damage to the product caused during shipping If damage is present contact carrier at once Failure to do so immediately may release the carrier from any liability of damage 2 Fill out the checklist page of instruction manual If something is missing please contact us 3 Carefully read this instruction manual before operating the RevCount 4 Fill out warranty registration card and mail it to APR amp D Inc Limitations of Use Denville Scientific is not responsible for misuse of the RevCount ALWAYS clean the RevCount with a damp cloth and appropriate mild deter gent solution Never clean RevCount with organic solvents like thinner or ben zene It will damage the surfaces CAUTION This is the only recommended method of cleaning 2 Never attempt to dismantle or modify the RevCount 3 Never operate the RevCount with a damaged USB cord or after the appliance malfunctions or has been damaged in any manner 4 Accessory attachments not recommended by Revolutionary Science may damage the RevCount or tablet cpu 5 Never place the RevCount or any part of the RevCount in an environment that exceeds 65 degrees Celsius o
4. October Hill Road Holliston MA 01746 19 DENVILLE SCIENTIFIC INC a division of Harvard Bioscience Inc Zoom The RevCount software uses what is typically known as pinch and zoom meth od To zoom in ona spot simply touch the screen with two fingers together and then slide them apart To zoom out on an area touch the screen with two fingers apart and then slide them together ss e d Zoom out Zoom in Manually add cells Tap on the Manual tab at the top and then on Add Live Cells Then tap on the image where you want to add a new counted cell The new counted cells will show up as a white dot as apposed to the green dots Note added cells will show up as a positive figure under Cells added in the notes section on the right side of the screen Manually remove cells To remove a live highlighted cell press and hold finger on cell to remove it This will now show a red X To remove a dead highlighted cell press and hold finger on cell to remove it This will now show a black X WWwW densci com info densci com e Fax 508 429 5732 i19 800 453 0385 84 October Hill Road Holliston MA 01746 20 DENVILLE SCIENTIFIC INC a division of Harvard Bioscience Inc Selecting a Previous Image Data Tap on File and then tap on Load Image The Load Image list of previous imag es will appear Select desired image and confirm Saving Data Tap on File and then tap on Capture Image Th
5. _ Detachable power supply w USB connector Manual Notes amp Service Record For your own future reference and to expedite answers to your questions please complete the following information Date Purchased Serial Number Model Number Purchased From Your Notes www denscli com info densci com e Fax 508 429 5732 i19 800 453 0385 84 October Hill Road Holliston MA 01746 DENVILLE SCIENTIFIC INC a division of Harvard Bioscience Inc Warranty Registration Please fill out this page or a copy of it and send it to the address below to validate your warranty Warranty Registration Card for the Denville Scientific RevCount Please Print Date Purchased Serial Number Purchased From Please check one Company School Individual Name of Department or Institution Address Intended use Where did you hear of our product Comments Warranty Policy Your Denville Scientific RevCount is warranted to be free from defects of material or workmanship under normal use for a period of two years from date of purchase If your RevCount proves to be defective within this warranty period APR amp D Inc will repair or replace your unit at our option This warranty will be void if malfunction is caused by accident misuse or negligence including tampering abuse or damage in transit This warranty excludes any relief for incidental or conse quential damages www denscli com info densci com e Fax 508 429 5732
6. on the right side of the screen Simply slide finger up or down for adjustment Brightness Contrast www densci com info densci com e Fax 508 429 5732 i19 800 453 0385 84 October Hill Road Holliston MA 01746 15 DENVILLE SCIENTIFIC INC a division of Harvard Bioscience Inc When Preview is shown on top menu bar image displayed is live When Pause is shown on top menu bar image displayed is fixed The Pause mode is setting which all counting occurs Dilution Calculator Help Capturing Images To capture an image for counting cells tap the CAPTURE button under the File dropdown menu Note Before capturing you may need to adjust the focusing Add Live Cells ina 800 453 0385 www densci com info densci com Fax 508 429 5732 84 October Hill Road Holliston MA 01746 16 DENVILLE SCIENTIFIC INC a division of Harvard Bioscience Inc Cell Counting To count an image tap the COUNT button on the lower right side of screen The results will appear in the notes box on the right side of the screen In the image dead cells will be highlighted in black while live cells will be highlighted in green see Sensitivity and Cell Size sections on pages 16 and 7 Note Before counting you may need to adjust the sensitivity and or the counting region C3 File Counted Image Preview Manual Camera Controls Dilution Calculator Help Ul Jr Count Exclusion zone
7. DENVILLE SCIENTIFIC INC a division of Harvard Bioscience Inc The RevCount User Manual WWwW densci com info densci com e Fax 508 429 5732 i19 800 453 0385 84 October Hill Road Holliston MA 01746 DENVILLE SCIENTIFIC INC a division of Harvard Bioscience Inc Table of Contents Parts Checklist Notes Service Record 3 Warranty 4 Safeguards and Cautions Limitations of Use 5 Intended Use 7 Specifications Contact Information 8 Hardware Installation For RS RC 150 only 9 Quick Start I0 Step By Step Comprehensive Setup lO Wi Fi Setup 12 Automatic Updates 3 Cell Profile Setup 3 Setup Lighting Focusing Brightness and Contrast Controls 4 Live Video Feed l6 Capturing Images l6 Cell Counting 7 Exclusion Zone 7 Count Sensitivity 18 Cell Size I9 Zoom 20 Adding Removing cells from count 20 Selecting Previous Image 21 Saving Data 21 Export to PDF 21 Calculating Dilutions 22 Help 23 Appendix A Camera Settings 23 WWwW densci com info densci com e Fax 508 429 5732 i19 800 453 0385 84 October Hill Road Holliston MA 01746 DENVILLE SCIENTIFIC INC a division of Harvard Bioscience Inc This page intentionally left blank www densci com info densci com e Fax 508 429 5732 i19 800 453 0385 84 October Hill Road Holliston MA 01746 DENVILLE SCIENTIFIC INC a division of Harvard Bioscience Inc Parts Checklist _ RevCount _ USB Belkin Hub Power OTG cable Tablet cpu
8. ILLE SCIENTIFIC INC a division of Harvard Bioscience Inc Help The help menu has three items About will give company name and model information User Guide will open up this document Contact information for Denville Scientific s Technical support Email info densci com Telephone contact 800 453 0385 www densci com Help Help About Us User Manual Appendix A Camera Settings Focus Adjust focus for better clarity Brightness Adjusting Brightness lightens or darkens the image Contrast Adjusting contrast heightens or lessens the difference between light and dark are as in the image www densci com info densci com e Fax 508 429 5732 i19 800 453 0385 84 October Hill Road Holliston MA 01746
9. become available they can be installed seamlessly by swiping the notifica tion bar upper left hand corner of the screen and clicking Update Available option The update will then download onto the tablet Install the up date when download is complete sa 1 fS meem a A2VO0l uu Save Profile Data Count Cell Profile Setup Many of the settings can be programmed in and saved for each cell type being counted To make a cell profile simply adjust the camera settings p15 sensitivity settings p 18 and click Save ina 800 453 0385 www densci com info densci com Fax 508 429 5732 84 October Hill Road Holliston MA 01746 13 DENVILLE SCIENTIFIC INC a division of Harvard Bioscience Inc Focusing To focus the RevCount run the RevCount software insert sample and tap on preview button While viewing the sample turn the focusing wheel either clockwise or counter clockwise located in the front of the RevCount until the best focus is given WWwW densci com info densci com e Fax 508 429 5732 i19 800 453 0385 84 October Hill Road Holliston MA 01746 14 DENVILLE SCIENTIFIC INC a division of Harvard Bioscience Inc Brightness and Contrast Controls Make sure camera mode is in preview will show as 3rd tab on top menu bar To adjust the image brightness or contrast tap on Camera Controls and then tap on Manual controls The controls will then appear
10. e hemocytometer Cells outlined in green are counted live while those outlined in black are dead Those cells outlined in red are either to big or too small Limit the size of detected cells by Tapping Manual in the menu and selecting Set Cell Size Adjust cell size by moving the min and max size sliders to de sired parameters Cells outside of parameters will be outlined in red www denscli com info densci com e Fax 508 429 5732 i19 800 453 0385 84 October Hill Road Holliston MA 01746 10 SCIEN TIFIC INC a division of Harvard Bioscience Inc 1 Save the desired parameters by tapping Save This will save a profile that will be automatically a e MEA loaded the next time the RevCount app is used Minsee 2 12 Tap the count button to count the sample and Max Size 23 view the details of the count Threshold 6 3 If a PDF copy is desired Tap File then select eee Save PDF Contrast 64 14 To save a copy of the image tap File then Load Save select Capture Image Give the image a name and will be saved to the RevCount_images directory on the tablet 15 To save the count data tap File then Save Data 16 To perform a new count Tap the gt button to the right of Count A new image will be taken and counted with the previous saved parameters If the cam era needs adjusting again Tap Pause then select Preview 7 To receive automatic updates turn
11. ghting by use of the rotary switches in back 3 Click count Comprehensive I 2 3 4 gt 6 7 8 7 Connect the RevCount base to the Android tablet as shown on page 9 Make sure all components USB hub tablet RevCount base USB OTG cable pow er supply are all connected correctly Turn on the Android tablet by holding in the power button for a couple sec onds until the boot screen Google appears The device should boot up straight into the RevCount app Check the optics by placing a blank disposable hemocytometer into the stage of the RevCount base located below the focusing wheel see focusing page A view of the hemocytometer should appear on the screen Prepare a sample by pipetting 4uL of sample into either chamber of the hemocytometer and inserting it back into the stage on the RevCount base Adjust the focusing wheel to get the crispest image Adjust the cameral controls by tapping on Camera Contols in the menu bar then select Manual Controls Adjust the brightness and contrast by moving the slider bars in the dialog that opened up until the desired contrast and brightness is reached Pause the image by tapping Preview in the menu the selecting pause This will freeze the current frame in the camera for counting Move the Sensitivity Slider up or down until the best count is reached The counting area is in the center of the screen overlaid with a 4x4 grid which represents a mm square on th
12. is brings up a Saved As menu Type in desired name for the file and tap enter Export to PDF Tap on File in the top menu and tap on Save PDF This brings up a Saved As menu Type in desired name for the file and tap enter test sample 13 12 16 10 37 4 WwWwW densci com info densci com e Fax 508 429 5732 i19 800 453 0385 84 October Hill Road Holliston MA 01746 21 DENVILLE SCIENTIFIC INC a division of Harvard Bioscience Inc Calculating Dilutions To calculate dilutions tap on Dilutions Calculator in the top menu bar and then tap on Calculate Dilution Enter starting concentration and desired final concen tration then tap on Calculate to find volume of cell sample needed to be added to buffer solution For example to make 10 uL of a desired cell concentration of 1045 cells mL from an initial concentration of 106 cells mL type in 10 E 6 as the starting concentra tion 10 E 5 as the final concentration and lOuL as the final volume In the display window the instructions will be given to mix luL of cell sample suspension to 9 uL of buffer solution centration 1 0 E 6 cells ml centration 1 0 E 5 cells mL u Calculate add 1 0 uL of 1 0E6 cells mL original solution to 9 0 uL of buffer solution for a final volume of 10 0 uL and concentration of 1 0E5 cells mL www densci com info densci com e Fax 508 429 5732 i19 800 453 0385 84 October Hill Road Holliston MA 01746 22 DENV
13. on WI FI see p 12 Count File Image Pause Add Live Cells Camera Controls Dilution Calculator Help yeast3_14_1_30 13 23 23 jpg Live Cells Counted 736 Dead Cells Counted 7 max 22 00um min 2 00um avg 8 00um Live Cells Added 0 Live Cells Removed 0 Dead Cells Added 0 Dead Cells Removed 0 Intensity 6 Min Gate Size 2um Max Gate Size 23um Live Cell Concentration 7 4E6 cells mL Dead Cell Concentration 7 0E4 cells mL 13 Jun 2014 9 24 12 CDT gt Profile 5 www densci com info densci com Fax 508 429 5732 diri 84 October Hill Road Holliston MA 01746 41 DENVILLE SCIENTIFIC INC Wi Fi Setup To enable Wi Fi to receive automatic updates to the software swipe down on the wifi icon below in the upper right corner of the tablet screen Select the Wi FI option Select your network and type in your network key and press Connect When connected to the internet through wi fi the tablet can receive updates as they become available Signal strength Fair WPA WPA2 PSK inl a 800 453 0385 www densci com info densci com Fax 508 429 5732 84 October Hill Road Holliston MA 01746 12 DENVILLE SCIENTIFIC INC a division of Harvard Bioscience Inc Automatic updates The engineers at Denville Scientific are striving to make the feel and accuracy of the software better and better To this end we release updates from time to time As updates
14. r below 5 degrees Celsius Humidity should be at or below 65 6 Never use RevCount for any purpose other than the intended use www denscli com info densci com e Fax 508 429 5732 i19 800 453 0385 84 October Hill Road Holliston MA 01746 DENVILLE SCIENTIFIC INC a division of Harvard Bioscience Inc 8 Never attempt to modify the plugs or cords This may cause damage to the RevCount or tablet cpu 9 Never immerse the RevCount cord or plug in water or operate on wet sur faces 10 Never allow the cords to contact hot surfaces beyond 65 C Any slack in the cord should be wrapped up and held together with a band www denscli com info densci com e Fax 508 429 5732 i19 800 453 0385 84 October Hill Road Holliston MA 01746 DENVILLE SCIENTIFIC INC a division of Harvard Bioscience Inc Intended Use RevCount The Denville Scientific RevCount can be used for the following purposes Cell Counting e Count cells on disposable gridless hemocytometers e Distinguishes between live and dead cells using Trypan Blue stain e Determine live and dead cell concentrations www denscli com info densci com e Fax 508 429 5732 i19 800 453 0385 84 October Hill Road Holliston MA 01746 Specifications Power requirements Net weight Dimensions Cell Size Label or Dye Sample Volume Cell Concentration Slide Compatibility CPU Certifications Warranty Regulatory Statement UT 18 800 453 0385
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