Model 33 Twin Syringe Pump User`s Manual
Contents
1. 25cc 9 60 mm 0 25cc 3 45 mm 250 2 3 5 12 45 0 5 3 45 500 3 26 10 15 90 4 50 1 0 ml 4 61 mm 20 20 05 8 92 2 5 7 28 30 22 50 8 99 5 10 3 50 29 00 11 70 10 14 57 14 70 25 23 0 Unimetrics 19 58 50 32 6 Series 4000 amp 22 70 5000 29 00 Becton Dickinson 35 70 Glass All Types Size Diameter Diameter 0 460 mm Renfac 0 729 0 5 pl 4 64 mm 1 031 Diameter 1 4 64 1 460 9 12 mm 25 8 66 2 300 12 34 5 11 86 3 260 14 55 10 14 34 4 610 19 86 20 19 13 23 20 30 22 70 27 60 50 28 60 100 34 90 Publication 5390 001 REV D Harvard Apparatus Model 33 Twin Syringe Pump User s Manual Appendix B Nominal Min Max Flow Rates Minimum flow rates are taken from the smallest inside diameters and maximum flow rates are taken from the largest inside diameters of the syringes supplied by the eleven most widely used syringe manufacturers Nominal Minimum Maximum Flow Rates for Various Syringes Actual Limits will vary depending on manufacturer Syringe pil hr pl min ml hr ml min Size Min i Min Max Min Min 0 5 pl 0 0004 0 79 1 pl 0 0008 1 58 2 ul 0 0015 3 1 5 pl 0 0037 7 93 10 pl 0 0073 15 829 25 pl 0 0183 39 756 50 pl 0 0365 79 519 100 pl 0 0731 159 46 250 pl 0 1813 23 751 500 pl 1000 pl 0 7281 95 418 1 ml 0 7828 102 580 2 ml 2 8493 373 430 2 5 ml 1 8156 237 950 3 ml 2 5691 336 710 5 ml 4 9824 653 010 10 ml 7 2024 15 733 20 ml 12 5360 27 384 30 ml 16 1310 35 236 50 ml 22. Diameters The pump must be stopped when entering a diameter Diameter 1 is the diameter of both syringes except in Proportional mode In Proportional mode enter the diameter of each syringe separately Diameter 1 for syringe 1 and Diameter 2 for syringe 2 When entering a diameter the 2 direction LEDs corresponding to the syringe and the mm LED will blink Enter the inside diameter of the syringe in millimeters After a new diameter is entered the corresponding rate is set to 0 This is done for reasons of safety The maximum diameter is 50 mm See Appendix A for a list of syringe diameters Pumping Rates Rate 1 is the rate of pumping of both syringes except in Proportional mode In Proportional mode enter the rate of each syringe separately Rate 1 for syringe 1 and Rate 2 for syringe 2 When entering a rate the directional LEDs corresponding to the syringe and the current units LED will blink Use the corresponding rate key RATE 1 or RATE 2 to scroll through the rate units ml min ml hr ul min and ul hr The minimum and maximum rates permitted vary depending on the diameter of the syringe If an OOR message meaning Out of Range is displayed when entering a rate try using a different syringe for your application Selecting the Run Mode To select the run mode press the SELECT MODE key to advance the run mode LED indicator Advance the run mode indicator to the desired mode either Auto Stop Proportional or Continuous m
3. 5 digits plus 14 indicator LEDS Publication 5390 001 REV D s Harvard Apparatus Model 33 Twin Syringe Pump User s Manual Features Pressure and Speed Pump 33 can deliver up to 106 60 ml min with a 140 ml syringe and is capable of pres sures of up to 99 5 PSI with a 20 ml syringe Independent Syringe Diameters and Rates In the Proportional Mode separate syringe types and rates may be set Variable Syringes Enter the inside diameter of the syringes being used Modes of Operation Auto Stop Both syringes will operate at same rate and stop when either syringe reaches a limit stop Continuous Run With both syringes operating at same rate when end of travel is reached the pump automatically reverses the direction of travel for both syringes The external valves optionally attached to the syringes will be automatically activated or deactivated Proportional Pump operates as in Auto Stop but independent rates and diameters are set for each syringe Parallel Reciprocal Operation Syringes may pump in the same direction parallel or opposite directions reciprocal External Connections TTL Allows pump operations to be synchronized with external devices or by a person at a distance from the pump Direction of pump travel can be set via a TTL pin Also TTL pins are used to control an external valve for refilling and provide an output to indicate whether or not the pump is running Additional TTL pins are available for gener
4. Pump stalled 1 or 2 digit pump address Unrecognized command Command not presently applicable Entered value out of range All spaces ignored Maximum 5 digits per integer or floating point number Legend float gt ffffff f gt dl integer gt ddddd d gt 0 11213141516171819 b gt space cr ASCII 13 If gt ASCII 10 text gt any string of ASCII characters S es non terminal upper case terminals optional E select one either or Publication 5390 001 REV D Harvard Apparatus Model 33 Twin Syringe Pump User s Manual i Appendix D RS 232 Specifications Pump Chain Pump Port 1 Pump Port 2 pump chain Baud Rate 300 1200 2400 or 9600 Word Size 8 Parity none Stop Bits 2 A Computer control side Connection for remainder of Transmit Receive GND GND GND GND Transmit Receive UOUU UOUU Port 2 out Port 1 in Figure 9 RS 232 Specifications endix E TTL Connector Specs TTL Connections are via a 9 pin D sub type connector All inputs are internally pulled up to a logic high All user definable pins are controlled via the IN and OUT pump chain commands See Pump Chain Commands Pin Assignments i Vss Ground reference logic low Directional Indicator Low Syring 1 Infusing High Syringe 1 Refilling Pump Run
5. Massachusetts 01746 Phone 508 893 8999 Toll Free 800 272 2775 Fax 508 429 5732 E mail bioscience harvardapparatus com Web www harvardapparatus com Warner Instruments Inc 1125 Dixwell Avenue Hamden CT 06514 Phone 203 776 0664 Toll Free 800 599 4203 Fax 203 776 1278 E mail sales warneronline com Web Wwww warneronline com Canada Harvard Apparatus Canada 6010 Vanden Abeele Street Saint Laurent Quebec H4S 1R9 Phone 514 335 0792 Toll Free 800 361 1905 Fax 514 335 3482 Toll Free 800 335 0792 E mail sales harvardapparatus ca Web www harvardapparatus ca U K Harvard Apparatus Ltd Fircroft Way Edenbridge Kent TN8 6HE Phone 44 1732 864 001 Fax 44 1732 863 356 E mail sales harvardapparatus co uk Web www harvardapparatus co uk France Harvard Apparatus S A R L 6 Avenue des Andes Miniparc Bat 8 F 91952 Les Ulis Cedex Phone 33 1 64 46 00 85 Fax 33 1 64 46 94 38 E mail info harvardapparatus fr Germany Hugo Sachs Elektronik Harvard Apparatus GmbH Gruenstrasse 1 D 79232 March Hugstetten Phone 49 0 7665 92 00 0 Fax 49 0 7665 92 00 90 E mail Info hugo sachs de Web www hugo sachs de
6. attached to this output enables automatic selecting of infuse and refill vessels Running Indicator Output Provides a signal to another device indicating whether or not the pump is running Publication 5390 001 REV D 4 Harvard Apparatus Model 33 Twin Syringe Pump User s Manual Pump Chain Commands The Pump Chain RS 232 interface is used to enhance the control applications of the Pump 33 This interface allows all control information to be entered into the pump from an external source such as a computer In addition this interface allows up to 100 Pump 33 s or in certain cases other RS 232 devices to be controlled from a single RS 232 communication port on a computer Assign each pump in the pump chain a unique address from 0 to 99 This address is used to identify which pump is to receive a command and which pump is responding Configure each pump with its assigned address and the baud rate being used See External Control amp Interfaces When a pump is sent a command or a request is made for its prompt the Remote LED on the display will illuminate indicating that the pump is receiving pump chain commands The LED remains illuminated until the pump is turned off or SET and 1 is entered on the keypad indicating a change in the pump chain configuration A pump will not respond to pump chain communication while it is in a setting mode entered when the user presses the SET key The pump can still be controlled from the keypad while
7. cause of stalling and then press RUN STOP key to resume pumping Single Syringe Operation To operate only one syringe select the Proportional mode and set the rate of the syringe not in use to 0 Set the diameter and rate of the other syringe to discard values www harvardapparatus com 3 Harvard Apparatus Model 33 Twin Syringe Pump User s Manual Valve Control Box Setting up Valve Set up consists of 1 Pump to Valve Electrical Connections The cable fitted with a male 9 pin D sub connector must be connected to the female D sub connector on the rear of the Model 33 Syringe Pump The Valve Power can now be swiched on with the illuminated power On Off switch on the front valve housing 2 Valve tubing to syringe hook up The valve s are de energized when the front mechanism 1 is running in the INFUSE direction The syringe on the 1 mechanism when infusing should therefore be connected to the lower tubing in the pinch valve see fig ures on next page Continuous Delivery Valve Boxes Model No Description 55 7000 Pump 33 Continuous Delivery Valve Box normal pressure 30 p s i supplied with 3 2 mm ID X 6 4 mm OD 1 8 ID X 1 4 OD silastic tubing and a connector cable for connection to the syringe pump 55 7001 Pump 33 Continuous Delivery Valve Box high pressure 200 p s i supplied with 6 4 mm 1 4 OD stainless steel tubing with SWAGELOK fittings and connector cable for connection to the syringe pump Publicat
8. direction to infusion DIR REF Sets syringe 1 pumping direction to refill DIR REV Reverses pumping direction of syringe 1 Direction of syringe 2 is set appropriately www harvardapparatus com 7 Harvard Apparatus Model 33 Twin Syringe Pump User s Manual Pump Chain Commands Query pumping direction of syringe 1 DIR Response will be one of INFUSE REFILL PAR ON OFF Request to set or query Parallel or Reciprocal setting SET Command Meaning PAR ON Sets syringes to parallel pumping direction PAR OFF Sets syringes to reciprocal pumping direction Direction of syringe 2 changes appropriately while direction of syringe 1 does not change Query PAR Possible Responses Meaning ON Parallel OFF Reciprocal IN lt d gt Request to read in the TTL logic level at the specified pin d on the external 9 pin D SUB connector Valid pin numbers for input are 6 7 and 8 Possible Responses Meaning ON TTL level high OFF TTL level low If the pin specified is invalid a Not Applicable error is returned OUT lt d gt ON OFF Request to set the TTL logic level at the specified pin d on the external 9 pin D SUB connector ON Set pin to a logic high OFF Set pin to a logic low Valid pin numbers for output are 4 and 5 If the pin specified is invalid a Not Applicable error is returned SAV Request to save pump settings in the pumps non volatile memory NOVRAM Normally setting
9. enter the new data value Up to five digit numbers are accepted for rates and diam eters including up to four decimal places Entering more than five digits will clear the previous five digits in the display Press the green ENTER key when the desired data value is displayed If the data value entered is outside the pump s operating parameters the display will read oor meaning Out of Range Pressing any key will restore the display with the original data value Enter another data value within the pump s parameters or just press ENTER to reuse the original data value The data value entered can be reviewed as described above Note Certain data items have multiple settings For these after the ENTER key is pressed the display will prompt you for the additional information Various rules apply to when what and how data can be set at various times See the relevant section for further details Toggle Keys The keys in this group when pressed successively select different states Each state change is accompanied by a corresponding change in status LED display To operate Pump 33 needs to know the diameter s of the syringes the rate s of pumping pumping directions and the mode of operation Except for Proportional mode syringe 2 is assumed to be the same diameter as syringe 1 and will pump at same rate as syringe 1 Publication 5390 001 REV D 5 Harvard Apparatus Model 33 Twin Syringe Pump User s Manual Operation
10. hr ul mn ul hr Publication 5390 001 REV D Pump Chain Commands 9 f Harvard Apparatus Model 33 Twin Syringe Pump User s Manual DIA A B lt float gt Request to set or query syringe diameter settings Set syringe 1 diameter DIA diameter DIA A diameter Set syringe 2 diameter only valid in proportional mode DIA B diameter Corresponding syringe s rate will be zeroed Diameter is of the format ffffff Units are millimeters Diameter 2 is only accepted when mode is proportional If diameter is accepted and valid diameter will become the new diameter Diameter will not be accepted if the pump is running and a Not Applicable error is returned If the diameter is invalid an Out Of Range error is returned Query syringe 1 diameter DIA DIAA Query syringe 2 diameter only valid in proportional mode DIA B Response is of the following format ffffff Units are millimeters MOD AUT PRO CON Request to set or query pumping mode SET Command Meanin MOD AUT Puts pump in Auto Stop mode MOD PRO Puts pump in Proportional mode MOD CON Puts pump in Continuous mode If pump is running command will not be accepted and a Not Applicable error is returned Query MOD Possible Responses Meaning AUT Auto Stop mode PRO Proportional mode CON Continuous mode DIR INF REF REV Request to set or query pumping direction of syringe 1 SET Command Meaning DIR INF Sets syringe 1 pumping
11. it is in a pump chain Control data that is changed via RS 232 will NOT be stored in the pump s non volatile memory unless requested with the SAV command or other data is changed from the keypad Each command sent to the pump chain is a string of ASCII characters with leading zero s on numbers and all spaces optional Numbers are a maximum of five digits The following symbols are used in describing the commands Symbol Meanin ese optional ee select one either or f digits 0 9 or a decimal point d digits 0 9 lt cr gt carriage return ASCII 13 lt lf gt line feed ASCII 10 lt float gt ffffff lt integer gt ddddd lt text gt any string of ASCII characters Command Formats Meaning lt cr gt Stops all pumps All pumps on the pump chain interpret this as a stop command but do not respond with a prompt pump address lt cr gt Request for prompt The pump with the indicated address responds with its prompt optional pump address Sends a command to a pump The pump command lt cr gt with the indicated address executes the command then responds with its prompt The optional pump address if not speci fied will default to pump address 0 After each command is received and executed the pump acknowledges the command with a prompt Preceding the prompt will be the text response if applicable The text response will be one or more lines of ASCII text each preceded by a line feed and ter minated by a
12. pressure 30 p s i supplied with 1 8 ID x 1 4 OD silastic tubing and connector cable for connection to the syringe pump Continuous Delivery High Pressure Valve Box high pressure 200 p s i supplied with 1 4 OD 304 stainless steel tubing with SWAGELOK fittings and a connector cable for connection to the syringe pump www harvardapparatus com 3 Harvard Apparatus Model 33 Twin Syringe Pump User s Manual Appendix J Custom Applications The Harvard 33 Syringe Pump lends itself to a multitude of OEM industrial applica tions for all types of custom pumping or pilot plant applications Please contact the Harvard Technical Support Department if we can be of help Publication 5390 001 REV D Declaration of Conformity Application of Council Directive s Standard s to which conformity is declared Manufacturer s Name Manufacturer s Address Type of Equipment Model No 73 23 EEC 89 336 EEC EN61010 EN50082 1 EN50081 1 Harvard Apparatus Inc 84 October Hill Road Holliston Massachusetts 01746 U S A Syringe Pump Model 33 I the undersigned hereby declare that the equipment specified above conforms to the above Directive s and Standard s Place Date United States of America November 11 1996 Signature Beth Bauman Full Name VP Engineering Operations Position HARVARD APPARATUS U S A Harvard Apparatus 84 October Hill Road Holliston
13. 4 4201 53 346 100 ml 0 087 91 20 140 ml 0 1053 106 60 www harvardapparatus com 4 Harvard Apparatus Model 33 Twin Syringe Pump User s Manual Appendix C Command Information Pump Chain Command Summary All spaces in command are ignored Maximum 5 digits per integer or float data command gt lt adr gt lt cmd gt lt CR gt adr gt d d cmd gt RUN STP RAT A lt float gt lt units gt RAT B lt float gt lt units gt DIA A lt float gt DIA B lt float gt MOD AUT PRO CON DIR INF REF REV PAR ON OFF IN lt d gt OUT lt d gt ON OFF SAV VER units gt UM UH MM MH Command Response Output Format output gt lt response gt lt prompt gt response gt lt response gt lt response gt lt lf gt lt message gt lt cr gt lt lf gt lt adr gt lt lf gt lt adr gt gt lt lf gt lt adr gt lt lt lf gt lt adr gt adr gt d d message gt NA OOR lt text gt lt integer gt lt float gt prompt gt 1 or 2 digit address Start pump Stop pump Set query syringe 1 rate Set query syringe 2 rate Set query syringe diameter 1 Set query syringe diameter 2 Set query pumping mode Set query pumping direction Set query Parallel Reciprocal Read TTL pin d Set TTL pin d Save settings in NOVRAM Query software version ul mn ul hr ml mn ml hr Stopped Syringe 1 infusing Syringe 1 withdrawing
14. 90 001 REV D s Harvard Apparatus Model 33 Twin Syringe Pump User s Manual User Interface Description of Keys Set Keys 1 Diameter 1 33 Syringe Pump Rate 2 Diameter 2 L Proportional Only m Change Direction Reciprocal Mode Parallel Select Toggle Data Entry Figure 3 Keypad Interface The keypad consists of 21 keys used for entering control information and data into the pump These keys are grouped into 3 sections see Figure 3 above Set keys toggle keys and data entry keys Set Keys Set Rate 1 Rate 2 Diameter 1 Diameter 2 q1 Toggle Keys Parallel Reciprocal Select Mode Change Direction Run Stop Allows modification of a data item in this group of keys To modify a data item press the relevant key after pressing the SET key Displays sets current rate of syringe 1 Also syringe 2 rate if current mode is not Proportional Scrolls through rate units when setting Displays sets current rate of syringe 2 Scrolls through rate units when setting Key only valid in Proportional mode Displays sets current diameter of syringe 1 Also diameter of syringe 2 if current mode is not Proportional Displays sets current diameter of syringe 2 Key only valid in Proportional mode Used to display and set current Pump Chain address and to scroll thro
15. Model 33 Twin Syringe Pump User s Manual Syringe Pump Model 353 MAI 55 3333 HARVARD APPARATUS Publication 5390 001 REV D WEEE RoHS Compliance Statement EU Directives WEEE and RoHS To Our Valued Customers We are committed to being a good corporate citizen As part of that commitment we strive to maintain an environmentally conscious manufacturing operation The European Union EU has enacted two Directives the first on product recycling Waste Electrical and Electronic Equipment WEEE and the second limiting the use of certain substances Restriction on the use of Hazardous Substances RoHS Over time these Directives will be implemented in the national laws of each EU Member State Once the final national regulations have been put into place recycling will be offered for our products which are within the scope of the WEEE Directive Products falling under the scope of the WEEE Directive available for sale after August 13 2005 will be identified with a wheelie bin symbol Two Categories of products covered by the WEEE Directive are currently exempt from the RoHS Directive Category 8 medical devices with the exception of implanted or infected products and Category 9 monitoring and control instruments Most of our products fall into either Category 8 or 9 and are currently exempt from the RoHS Directive We will continue to monitor the application of the RoHS Directive to its products and will comply with a
16. S and or DSR lines Verify pins 4 5 and 6 on the 25 pin connector are jumpered on the computer side of the cable Display Messages All digits flashing Pump has not received any commands since being powered on StALL Also a syringe LED and Run LED flashing This indicates that the pusher block travel of the syringe corresponding to the syringe LED blinking has been impeded This may be caused by the syringe plunger hitting bottom a kink in the tubing syringe plunger binding or any situation requiring more force to the head of the syringe than the pump is capable of delivering To restart the pump remove the cause of stalling and press RUN The pump will continue the interrupted procedure where it stopped Also note a large increase in the pumping rate while running could also stall the motor oor Out of range A value was entered or encountered in a pump program that was beyond the pumps limits Try using a different size syringe Note that rates must be less than 42950 Express larger rates using different units Key pressed is not applicable Publication 5390 001 REV D 7 Harvard Apparatus Model 33 Twin Syringe Pump User s Manual Appendix I Accessories Catalog No MA1 55 4144 MA1 55 7760 MA1 70 2022 MA1 55 7000 MA1 55 7001 Product Foot Switch Daisy Chain Cable 2ft for connecting two or more pumps need 1 cable per pump RS 232 Connector Cable 7ft 9 pin D Sub Continuous Delivery Valve Box normal
17. TIAL DAMAGES Some states do not allow exclusion or limitation of inci dental or consequential damages so the above limitation or exclusion may not apply to you THERE ARE NO IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR USE OR OF ANY OTHER NATURE Some states do not allow this limitation on an implied warranty so the above limitation may not apply to you If a defect arises within the two year warranty period promptly contact Harvard Apparatus Inc 84 October Hill Road Building 7 Holliston Massachusetts 01746 1371 using our toll free number 1 800 272 2775 or outside the U S 508 893 8999 Goods will not be accepted for return unless an RMA returned materials author ization number has been issued by our customer service department The customer is responsible for shipping charges Please allow a reasonable period of time for comple tion of repairs replacement and return If the unit is replaced the replacement unit is covered only for the remainder of the original warranty period dating from the pur chase of the original device This warranty gives you specific rights and you may also have other rights which vary from state to state Repair Facilities and Parts Harvard Apparatus stocks replacement and repair parts When ordering please describe parts as completely as possible preferably using our part numbers If practical enclose a sample or drawing We offer a complete reconditioning service CAUTION T
18. al use RS 232 Multiple pumps can be chained together and remotely controlled from a comput er or any device communicating via RS 232 Non Volatile Memory All operational data entered into the pump from the keypad or requested to be saved via RS 232 will be stored On power up all settings from when it was powered down will be recalled and the display will blink until the pump receives its first command Stall Detection An optical detector on each motor is used to verify expected movement of the motors If motor is prevented from turning due to jamming or kinking of the tubing the pump stops and the display indicates a stall condition by blinking the run light and the syringe direction light of the stalled syringe Pump 33 employs a microcontroller which controls a small step angle stepping motor that drives a lead screw and half nut Microstepping techniques are used to further reduce the step angle making flow pul sation negligible A keypad is used for entry of operating data to the pump or data can be entered via RS 232 The microcontroller calculates the cross sectional area of the syringe selected and calibrates the flow rate www harvardapparatus com 4 Harvard Apparatus Model 33 Twin Syringe Pump User s Manual Initial Setup 1 Read the manual 2 Locate the voltage selector switch on the rear panel of the pump and set it to the voltage being used If other than 115VAC 60 Hz is being used the plug must be cut off and an app
19. carriage return lt lf gt lt text gt lt cr gt www harvardapparatus com 3 Harvard Apparatus Model 33 Twin Syringe Pump User s Manual Pump Chain Commands A prompt is a string of ASCII characters sent by a pump indicating the pump s address and its present state lt lf gt 1 or 2 digit address prompt character Prompt Characters Meaning Pump stopped gt Syringe 1 infusing lt Syringe 1 refilling Pump stalled Pump Commands and Responses RUN Starts pumping according to the present setting of the pump If pump already pump ing a Not Applicable error is returned STP Stops pump if it was running If pump already stopped a Not Applicable error is returned RAT A B lt float gt lt units gt Request to set or query infusion rate settings Set syringe 1 rate RAT rate RAT rate units RAT A rate RAT A rate units Set syringe 2 rate only valid in proportional mode RAT B rate RAT B rate units Rate is of the format ffffff Units are one of Meanin UM ul mn UH ul hr MM ml mn MH ml hr Rate 2 is only accepted when mode is Proportional If rate is accepted and valid rate will become the new rate If the rate is invalid an Out Of Range error is returned Query syringe 1 rate RAT RATA Query syringe 2 rate only valid in proportional mode RAT B Response is of the following format f f f f f f lt units gt Where units are one of the following ml mn ml
20. dapparatus com 3 Harvard Apparatus Model 33 Twin Syringe Pump User s Manual External Control and Interfaces Attaching a Valve Control Accessory Box Attach the valve control cable to the pump s accessory valve connection see Figure 9 Refer to the Valve Control Box section TTL Devices The pump does not need to be configured to attach a TTL device To attach a TTL device simply plug the appropriate TTL connector into the 9 pin connector on the rear of the pump See Appendix E for wiring specifications Foot Switch Input Used to start and stop the pump Pressing the foot switch performs the same function as pressing the RUN STOP key on the keypad The foot switch connector allows remote or automated operation of the pump Timer Input Opening the timer input starts the pump Closing the timer input stops the pump The timer input allows for an externally controlled pumping interval Pumping Direction Input Sets the direction of pumping Opening the directional input sets syringe 1 to infuse Closing the input sets syringe 1 to refill The pumping direction input is not recognized when data is being entered from the keypad Syringe 2 changes direction appropriately Also the valve control output is set appropriately Directional Indicator Output The Directional Indicator output is an indicator of the direction of pump travel When the output is a logical high syringe 1 is set to infuse A logical low indicates refill A valve
21. essages ccn 21 Appendices A Syringe Diameters in MM c cc eeceeeteeeenteeeeeees 22 B Nominal Min Max Flow Rates 23 C Command Information 22222222 zerg arr ub abs 24 D RS 232 Specifications u sssceeserrerrrrrenrrrrenrnnne 25 E TTL Connector Specifications cceeeeeees 25 F Pump to PC Connection c ccccccccscecteeeeeeeesneteees 26 G Maintenance eaea E E E 27 H Troublesho Qing rare la ae ia 28 l ACCOSSONO S arrestere ED Eee 29 J Custom Applications sssceererererrrrn rreren 30 www harvardapparatus com Harvard Apparatus Model 33 Twin Syringe Pump User s Manual Warranty and Repair Information Serial Numbers All inquires concerning our product should refer to the serial number of the unit Serial numbers are located on the rear of the chassis Calibrations All electrical apparatus is calibrated at rated voltage and frequency While the flow will stay calibrated the peak will vary Warranty Harvard Apparatus warranties this instrument for a period of two years from date of purchase At its option Harvard Apparatus will repair or replace the unit if it is found to be defective as to workmanship or material This warranty does not extend to damage resulting from misuse neglect or abuse normal wear and tear or accident This warranty extends only to the original customer purchaser IN NO EVENT SHALL HARVARD APPARATUS BE LIABLE FOR INCIDENTAL OR CONSEQUEN
22. his pump is not registered with the FDA and is not for clinical use on human patients AN CAUTION Not for clinical use on human patients Publication 5390 001 REV D s Harvard Apparatus Model 33 Twin Syringe Pump User s Manual General Safety Summary Please read the following safety precautions to ensure proper use of your syringe pump To avoid potential hazards and product damage use this product only as instructed in this manual To Prevent Hazard or Injury Use Proper Line Cord Use only the specified line cord for this product and make sure line cord is certified for country of use Ground the Product This product is grounded through the grounding conductor of the power cord To avoid electric shock the grounding conductor must be connected to earth ground Before making any connections to the input or output terminals of the product ensure that the product is properly grounded Make Proper Connections Make sure all connections are made properly and securely Any signal wire connections to the unit must be no longer than 3 meters Observe all Terminal Ratings Review the operating manual to learn the ratings on all connections Use Proper Fuse Use only specified fuses with product Avoid Exposed Circuitry Do not touch any electronic circuitry inside of the product Do Not Operate with Suspected Failures If damage is suspected on or to the product do not operate the product Contact qual ified service personnel
23. ion 5390 001 REV D Pinch Valve Connectors 3 Harvard Apparatus Model 33 Twin Syringe Pump User s Manual Top Tube z O z Bottom Tube SA a Valve Shown de energized Figure 4 Pinch Valve Connections lt _ Top Top _ gt gt Output Rear Bottom Bottom P Top Top Front i Fill Figure 5 Continuous Delivery Pump in Reciprocal Mode Top Top Output H Rear Rear Bottom Bottom P Top Top Output H Front Front Bottom Bottom Fill Fill Front Rear Figure 6 Dual Delivery followed by Refill Pump in Parallel Mode www harvardapparatus com gt Harvard Apparatus Model 33 Twin Syringe Pump User s Manual Stainless Steel Valve Connectors P Com B Normally Open A Normally Closed Output Front be Figure 7 Continuous Delivery Pump in Reciprocal Mode B Output Rear r D P A Output Rear Front B D P A Front Fill t j Fill Front Rear Figure 8 Dual Delivery followed by Refill Pump in Parallel Mode Swage lock fittings should be finger tight plus 1 1 4 turns Tubing is 304 stainless 1 4 OD wall thickness 0 035 Publication 5390 001 REV D External Control and Interfaces 5 Harvard Apparatus Model 33 Twi
24. isplay consists of a 5 digit LED display and 14 LED indicators The display will be showing either the default display or an informational message The two syringes are referred to as Syringe 1 and Syringe 2 Syringe 1 is located towards the front and Syringe 2 is located towards the rear Rate 1 refers to the set flow rate of Syringe 1 and Rate 2 refers to Syringe 2 By default the display will be showing Rate 1 Informational messages are shown when the user queries another data value by pressing the corresponding key or when the pump is alerting the user to a problem Such problems are a value out of range oor or the pump stalling StALL The 14 LED indicators are divided into three sections Pump mode and state syringe directions and units of value being displayed See Figure 2 The LED indicators are as follows Pumping Mode and State LEDs Meaning Auto Stop Pump is in Auto Stop mode Proportional Pump is in Proportional mode Continuous Pump is in Continuous mode Remote Pump Chain communication Run At least one motor is operating Syringe Directions LEDs Comprising of 4 indicators these LED s indicate the direction of the syringes when illuminated When blinking indicates the corresponding motor is stalled or data pertaining to that syringe is being entered Unit LEDs Meaning ml min milliliters per minute ul min microliters per minute ml hr milliliters per hour ul hr microliters per hour mm millimeters Publication 53
25. n Syringe Pump User s Manual TTL VOLTAGE SELECT PORT 2 PORT 1 Figure 9 External Interface External devices that can be attached to the Pump 33 include external valves pump chain and TTL devices See the appropriate appendixes for specification details on attaching devices Attaching a Pump Chain On the back of the pump are two telephone jack type connectors These are the RS 232 ports Looking at the back of the pump the connector on the right is port 1 and the left is port 2 Attach the RS 232 connectors in the appropriate port according to the following chart Device Port Number Computer 1 Pump Chain 2 Configuring the Pump Chain After pressing SET and 1 the Remote LED will blink indicating the pump is request ing its pump chain configuration 1 First the display will show Adr nn with the colon blinking where nn is the current pump chain address Enter the 2 digit address assigned to the pump and press ENTER Note Each pump in the chain needs a unique address 2 Next the display will show b nnnn with the colon blinking where nnnn is the current baud rate Use the 1 key to select between the supported baud rates 300 1200 2400 and 9600 then press ENTER Note Each pump in the chain must have the same baud rate See the section on Pump Chain Commands for pump chain control information www harvar
26. ning Indicator Low Pumped Stopped High Pump Running User Definable Output User Definable Output Foot Switch Input Falling edge starts stops pump Timer Input Rising edge starts pump Directional Indicator Running Indicator al Foot Switch 4 Pr 7 User Definable Outputs _ Vcc 5V Timer Refill Figure 10 TTL Connector Specifications Falling edge stops pump Directional control input Rising edge sets Syringe 1 to infuse Logic High Falling edge sets Syringe 1 to refill Vcc Logic High Reference Logic Low OV 05V Max 2 mA current sink 2V 5V Max 400 mA current source www harvardapparatus com Harvard Apparatus Model 33 Twin Syringe Pump User s Manual Appendix F Pump to PC Connection cp d Computer Terminal Port Settings Baud Rate 9600 or match pump baud rate Data Bits 8 Stop Bits 2 RJ11 Cable Straight Thru Pinning Max Distance 15M DB9 Female to 4 6 PIN RJ11 12 such as L COM P N RBA098F Computer Front View Fa STD DB9 2 RD 3 TD 5 Sig ground Connection Chart RJ12 6x6 RJ11 RJ12 DB9 3 STD DB25 2 TD 3 RD 7 Sig ground Publication 5390 001 REV D 4 Harvard Apparatus Model 33 Twin Syringe Pump User s Manual Appendix G Maintenance Pump 33 requires no special maintenance other than keeping it clean by avoiding acci dental spills of pumped material The two guide rods and the lead screw should be
27. ny changes as they apply Do Not Dispose Product with Municipal Waste e Special Collection Disposal Required 5 Harvard Apparatus Model 33 Twin Syringe Pump User s Manual Table of Contents Warranty and Repair Information ccccccesseeeeeeeeeeeeeee 2 General Safety Summary cccccessseeeeeeeceeeeeeeeeeeneneeeeneee 3 Specifications wiecccccsiciiis coccccasssceccscnd cencscececsccssnasscccsssesesas coe 4 FO AUN CS asaan A aAa ANERER AA RAA 5 Initial Setup sssssssssssnnssnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn nnmnnn nnna 6 Loading Syringes succeer EL nanmanna 7 User Interface Description of Keys ccccecsseessseesseeeeeeeeeeeeeeeeseeeeeeeeee 9 Entering Data ceirean aPN ERER RAEAN EEEN 10 Operation BE a e P E deste E T E E 11 PUMPING Rates surrender 11 Selecting the Run Mode eseeeessseceesseeeeeeeeeeees 11 Selecting the Pumping Directions aececi 11 RUNNING the PM raa nes Sere titers ceed 11 Single Syringe Operation ce eeeeeeeseeceeeneeeeesneeeees 11 Valve Control BOX 22244 EEN ES ERE 12 Pinch Valve Connectors cccscsssseeeeceeseeeeeeeeeeeseeeneeee 13 14 External Control and Interfaces Attaching Configuring a Pump Chain sese 15 TIL Devices is is scccseteteedevsivecdsciestenenectecsieceeek cadecsnannewaees 16 Foot Switch Input ceeeeeceeeeeeeeeeeeeeeeeeeeneeeeeeeseneaas 16 Pump Chain Commands Pump Commands and Responses n 18 Pump Chain Error M
28. o move freely so the syringe to be loaded Loosen the screws on the syringe block and pusher block to free the retaining brackets 2 and 3 Figure 1 To free the pusher block from the leadscrew turn the knob on the front of the block 1 until the pin in the knob slips into the hole in the block The syringe clamp locking screw on the right side of the syringe block 4 should be loosened and the clamp rotated to the side Place the syringe barrel on the syringe holder block and move the pusher block to accommodate the plunger Make sure the syringe barrel flange and the plunger flange are held by the retain ing clamps Press the retaining brackets firmly against the flanges and tighten the retaining screws Rotate the syringe clamp and press down firmly on the syringe barrel Secure in place by tightening the locking screw 4 Setting Limit Stops To set the limit stops loosen thumbscrews on limit stops and slide stops to desired travel limits infuse limit amp refill tighten thumbscrews www harvardapparatus com 3 Harvard Apparatus Model 33 Twin Syringe Pump User s Manual User Interface Auto Stop ml min Proportional lt lt Syringet gt l min Continuous i ml hr Remote lt Syringe 2 gt pl hr Run mm Figure 2 User Interface The user interface consists of a display area and a keypad The d
29. ode Auto Stop Both syringes pump according to DIAMETER 1 and RATE 1 until syringe 1 reaches the limit switch pump stops Proportional Each syringe pumps independently syringe 1 according to DIAMETER 1 and RATE 1 syringe 2 according to DIAMETER 2 and RATE 2 When syringe 1 reaches limit switch pump stops Continuous Both syringes pump according to DIAMETER 1 and RATE 1 When syringe 1 reaches limit switch the direction of pump ing for both syringes is reversed and pumping continues The valves if applicable are set accordingly Selecting the Pumping Directions Parallel or Reci Pressing the PARALLEL RECIPROCAL key reverses procal Operation pumping direction of syringe 2 Change Direction Pressing the CHANGE DIRECTION key reverses the pump ing directions of both syringes When valves are used in the system then the valve s setting s will be changed simulta neously with the change in direction Running the Pump Pressing RUN STOP key starts pump and illuminates Run LED on display Pressing RUN STOP key again stops pump While running a new rate can be set The new rate for relevant syringe s takes effect when ENTER key is pressed Pump will operate according to selected run mode Pump can also be started and stopped via external controls If operation of one of the motors is impeded both motors will stop and dis play will read StALL with the run LED and the direction LED of stalled syringe blink ing Investigate
30. ropriate plug installed observing the polarity of the interna tional line cord used Brown high Blue neutral Green ground 3 Turn on main power switch located directly above the line cord on the rear panel The display will illuminate indicating that the power connections are cor rect The flashing display indicates that power has just been applied 4 Load syringes Set syringe ID NO Mm Set pumping rate s 7 Select the run mode 8 Selecting the pump direction See section on operation for more information Publication 5390 001 REV D Harvard Apparatus Model 33 Twin Syringe Pump User s Manual Loading Syringes SYRINGE 1 o eee ee oe Se LA Sates REFILL INFUSE LIMIT so NS LIMIT so NG LIMIT ses SWITCHES SYRINGE 2 ie te ad Betis ee et S oO Figure 1 Syringe Loading The syringe holder and pusher block are fitted with movable retaining brackets which hold the syringe barrel and plunger when refilling When loading the syringe into the pump it is necessary to adjust these brackets The pusher block is fitted with a mechanism to release the drive nut from the leadscrew This allows the block t
31. s changed from a pump chain are not stored in the pump s NOVRAM and are lost after power down VER Request for version of pumps embedded software Response for the present version will be 33V2 0 Publication 5390 001 REV D Harvard Apparatus Model 33 Twin Syringe Pump User s Manual Pump Chain Commands Pump Chain Error Messages Error messages are in the format lt lf gt lt message gt lt cr gt and are followed by a prompt lt Message gt is one of the following Possible Responses Meaning Syntax error in a received command NA Command Not Applicable at this time OOR Out Of Range Control data is out of pump s operating range www harvardapparatus com Harvard Apparatus Model 33 Twin Syringe Pump User s Manual Appendix A Syringe Diameters in mm Harvard Terumo SGE Scientific Stainless Steel Diameter Glass Engineering Diameter 8 95 mm Size Diameter 9 525 mm 13 00 25 ul 0 73 mm 19 130 15 80 50 1 03 28 600 20 15 100 1 46 34 900 23 10 250 2 30 44 755 29 10 500 3 26 Becton Dickinson Sherwood Monoject SE mi Se Tr Plastic Plasti Plastic 5 10 30 bak i Diameter 10 14 57 Size Diameter 4 78 mm ey mm Hamilton Microliter 8 66 12 70 Series Gastight 12 06 15 90 Size Diameter 10 14 50 20 40 05 pl 0 103 mm 19 13 23 80 0 1457 30 21 70 26 60 0 206 50 60 26 70 38 40 0 3257 Air Tite Popper amp Sons Inc lg All Plastic Perfektum Glass 1 031 Size Diameter Size Diameter 100 1 46
32. sparingly lubricated periodically with the Magnalube G R grease provided with the pump This Teflon R based grease is available either from Harvard Apparatus or Carleton Stuart Corp 13 02 44th Ave Long Island City NY 11101 Solvents of any type should never be used to clean the pump A mild detergent solution may be used to clean the keypad Fuse Replacement Make sure power cord is disconnected from main supply before replacing fuse Remove bottom cover on pump by removing four 4 rubber feet and four 4 screws Locate fuse on power supply module Remove fuse from fuse clip Use caution no to break fuse when removing Replace fuse bottom cover screws and rubber feet For continued fire protection replace fuse only with 250V fuse of the specified type and rating GAG 1AMP 250V SLO BLO FUSE 1 AMP S B Figure 11 Fuse Replacement WARNING Use in manner not specified by manufacturer may impair protection provided by equipment www harvardapparatus com i Harvard Apparatus Model 33 Twin Syringe Pump User s Manual Appendix H Troubleshooting RS 232 Difficulties Verify that the baud rates and data framing parameters on all devices are the same With a pump chain a Communication timeout error on a computer is usually caused by the computer errantly handshaking on the RTS C
33. to perform inspection Place Product in Proper Environment Review the operating manual for guidelines for proper operating environments Observe all Warning Labels on Product Read all labels on product to ensure proper usage CAUTION Protective Ground Refer to Manual Terminal www harvardapparatus com Harvard Apparatus Model 33 Twin Syringe Pump User s Manual Specifications Harvard Pump 33 Specifications Type Accuracy Reproducibility Syringe Type Size Minimum Size Maximum Flow Rate Minimum Maximum Non Volatile Memory RS 232 TTL Average Linear Force Drive Motor Motor Drive Control Motor Steps per Rev of Leadscrew Step Resolution Step Rate Minimum Maximum Pusher Travel Rate Minimum Maximum Power Voltage Range Dimensions H x W x D Weight Timing Belt Drive Lead Screw Pitch Display Microprocessor dual drive single syringe infuse withdraw 0 35 0 1 Plastic glass or stainless steel 0 5 pl 140 ml 0 0004 ul hr 106 6 ml min Storage of all settings RJ11 4 conductor 9 pin connector 57 Ibs 2 motors each 0 9 step angle motor Microprocessor controlled from 1 2 to 1 4 microstepping 1 600 steps at 1 2 stepping or 3 200 steps at 1 4 stepping 0 33 um step 27 3 sec step 416 7 psec step 0 726699 u m min 95 25 mm min 45 W 1 0 A fuse 95 to 130 VAC 60 Hz 220 to 260 VAC 50 Hz selectable 15 2 x 31 1 x 28 6 cm 6 x 12 5 x 11 25 in 6 8 kg 15 Ib 2 1 24 threads inch
34. ugh supported baud rates while setting Chooses between Parallel and Reciprocal pumping operation Chooses between Proportional Continuous Run or Auto Stop LED indicators display current mode Pump must be stopped to change modes Reverses direction of both syringes and sets the valve control output as appropriate Starts or stops the pump Data Entry Keys 1 through 0 Enter Used to facilitate the entering of numeric data values Stores displayed data value when entering data www harvardapparatus com 5 Harvard Apparatus Model 33 Twin Syringe Pump User s Manual Entering Data Set Keys The keys in this group are used to modify or review settings of the pumps control d a t a To review the current setting of a control data item simply hold down the relevant key and the data setting will appear in the display To modify a data setting first press then release the green SET key The display will then read SET Press the key in the SET key group of the data item that is to be mod ified The display will show the current setting Data is entered into the pump by either entering a numerical value or in the case of entering a baud rate by scrolling through a menu of choices Always press ENTER to terminate each data request by the pump When entering a numerical value the LEDs corresponding to the data being entered will blink Using the yellow data entry keys on the right side of the keypad see Figure 3
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