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Bedienungsanleitung Photometer 5010

1. Measure time T2 Delay T3555 etti rp nint Reagent time 72 sss Reagent time 7 3 suss C F AAsgp sp2 AAst sbi PERE AA given entering et ate with without entering 0 10s to 255s entering 0 to 1800s Vua entering 0 to 1800s n entering 0 to 1800s ipe entering 0 to 1800s PHOTOMETER 5010 5100 V5 10a 05 07 07 D LAB RIELE GMBH CO KG DATE 07 10 07 TIME 07 16 31 METHOD 14 C F DELTA PROGRAM 14 FACTOR 1 000 WAVELENGTH 405nm TEMPERATURE 37C TIME DELTA 12s MEASURE TIME 100s DELAY 10s REAGENT 2 40s REAGENT 3 60s UNIT U L MEASURE BLANK Sb A S A E1 0 083 0 411 0 1 2 0 110 0 382 3 0 146 0 492 0 Sb A S A E2 1 0 091 1 090 2 0 140 0 991 3 0 200 1 165 NO RESULT 1 0 671 2 0 578 3 0 619 N Sb A S A E1 0 1 0 000 1 012 2 0 000 1 138 3 0 000 1 076 0 Sb A S A E2 1 0 000 1 458 2 0 000 1 530 3 0 000 1 384 0 RESULT 1 0 446 2 0 392 3 0 307 Start method selection in the main menu See chapter 4 1 Measurement with programmed methods 4 2 Measurement with basic methods In the case of activated printer the print out of the method data follows The measuring window is shown Method procedure with sample blank gt Insert measure zero solution DMeasure all samples E1 maximum 25 Change to measuring E2 by E1 E2 DMeasure all samples E2 m
2. seeeeeene emen nne nrnen eere en nere enne nnns 13 3 5 1 Changing cuvette adaptors ette Tee iEn eiTe eee e da vi sodo e do bo ason kinn 13 3 5 2 Working with original flow through system ssseeeeee emm emen 13 3 5 8 Working with standard cuvettes ssssssssssssssssssssesesee eene rennen enne aou 14 3 5 4 Working with discrete flow through CUVEttE 0 00 0 2 cece cece ce cece ceeeeeae cece ee eeeeeaeeeeeeeseceneaeeeeeeeeeeeea 14 4 PROGRAM SELECTION comico 15 4 1 Measurement with programmed methods cecccceceeeeeeeeeeeaeeeeeeeeeeeeaeeeeeeesesaaaeaeeeeeseesenaeaeeeteess 15 4 2 Measurement with basic methods oocccconoccccnococcnononcnonanoncnn nono nono non cnn nano eene nnnm 16 4 3 Method SANON kise ve eko ak yn ka ik iaa np bk ei ki Bi a D n A n WAN boo Si kat ek aa l pa pat neste 16 4 4 Utlity DrOgEaITIS cuis etn aka kn Se ki ee cen tio seen cde vend Dna kon AE ae PE ao Moco 17 4 5 Lamp protection LAMP teden klou fan da de Dena ina kwa aka SEE a kd a oi dee exar kan 17 4 6 Line feed LE iaa 17 5 CALCULATION PROCEDURE S cccccccssseeeeeeeeeeeeeeseeseeceeeeeeeeeeseeeeeeeeeees 18 5 1 GENERAL NOTES iia 18 5 1 1 Fundamental to the handling reete ii 18 5 1 2 Fundamental to the tempering ooooococccnccocococcccconccononononcconcnnnnnononnnnncnnnnnnnnnncnncnnnnnnnnnccnncanannenes 18 5 1 3 Fundamental to the inputs wi
3. D S pu pum pa eH gn penn pr pq pem pr p on koi ko ko 1 p p p p p p e I e kr fil aaa laa ai e d Fe fil 7 2 12 User name ROBERT RIELE GmbH amp Co KG n n oo ee p p p p pm pp E ppm koni konn ed konn b b kon ed b all pu pe pp p a AR p p DC H Q a M p p p r p ES ES p la on aaa i aaa aaa aa laaa azil Page 58 The name of the laboratory can be stored permanently In case of a stored name an additional line within the header is sent to the printer or to the EDP By NEW the entry of the laboratory name is possible Enter the laboratory name via the alphanu meric keyboard Following functions are available e a 1 change to lowercase e 1 A change to numeric characters e A a change to uppercase e delete character e gt blank e ESC finish input without storage e ENT finish input with storage The names of maximum five users can be stored permanently After calling a method the user is queried In case of a stored name an additional line within the header is sent to the printer or to the EDP Select user by SELECT By NEW the entry of the user name is possible Enter the user name via the alphanumeric keyboard Following functions are available e a 1 change to lowercase e 1 A change to numeric characters e A a change to uppercase e dele
4. dark value is absolutely too high gt 16 bit or higher as the repeat dark level adjustment measurement check lamp filter check blank ADC overflow all multiplexer positions are too bright too dark at setting to repeat dark level adjustment zero check filter lamp check blank 7 mathematical overflow at measurement calculation check filter check standard check measuring solution ROBERT RIELE GmbH amp Co KG Page 64 9 ERROR MESSAGE CORRECTION 5010e 510 doc 16 01 08 8 check sum error in the data record of the dark offset repeat dark level adjustment Lt mes sum error in the data record of the device basic automatic error remedy setting status ADC correction division by a too small value 0 001 A check filter check standard check measuring solution 11 invalid calibration curve Select valid number setting to zero not possible zero value is 32768 cycle check lamp check filter check zero solution setting to zero not possible zero value is gt 983039 cycle check lamp check filter check zero solution 14 invalid standard measure valid standard solution no parameter memory vacant too little memory for non delete a no longer actual nonlinear linear methods method method no is occupied select other method no delete a no longer actual nonlinear method check sum error in the parameter memory nonlinear program method newly method E para OO overflow at Kinetic check me
5. EDIT CURVE After inquiry of the curve number all curve bases can be edited see fig 7 2 2 2 SHOW CURVE After inquiry of the curve number the function will be shown LIST The method number and the date of preparation of all current curves are shown EXIT Return to the utilities P and P Consecutive numbering of the current bases A C Switch input between A for absorbance and C for concentration E Accept the edited value Li Input and confirmation of a single 0 at A lead to the deletion of the current pair of points In order to set the value to zero enter e g 0 0 ESC End input and save curve data The bases are sorted in ascending order according to their A value The current status of the internal printer is indicated in the first line by OFF or ON Change setting by SELECT Save setting permanently by OK Save setting temporarily up to next switch off of the device by EXIT 7 UTILITY PROGRAMS 5010e 510 doc 16 01 08 7 2 4 Pump menu With these functions the pump and the bubble detector can be checked and adjusted The check and adjust ment is possible only with inserted flow through cuvette adaptor E r Baal ROBERT RIELE GmbH 8 Co KG Page 47 The menu offers following functions e Activation and deactivation of pump e Calibration of pump volume e Activation and deactivation of bubble detector and of the volume optimized mode e Media t
6. the cable of the bubble detector has a bad contact replace aspiration tube chapter 8 7 Se A CIT A e meme E not occupied motocupied ss motocupied ss m timeout printer internal the internal printer is n discon e Aa 53 Setofdatapointsismissing Set of data points is missing Check multi standard functions multi standard functions nonlinear measurement value out of range check the absorbance of the sample es evorat mise peaionlanoifer eat sermoe pomor er marl cooks OC dara notsioraie omien on merate Les ree moniy memo natounsetGc aeei unusea GC matado ss tee space cay memory rortonaaroc ame GE aay memoy ROBERT RIELE GmbH 8 Co KG Page 66 9 ERROR MESSAGE CORRECTION 5010e 510 doc 16 01 08 10 1 ENVIRONMENTAL CONDITIONS Climatic conditions for storage and transport of the packed device e Temperature 25 C to 70 C Relative humidity 20 to 85 e The Photometer 5070 must be used in an environment that meets the following conditions e Temperature 15 C to 35 C Relative humidity 20 to 85 Not exposed to direct sunlight or other source of direct light e g a spot light e Well ventilated area Free from excessive dust e Free from combustible gases Free from vibrations Free from electromagnetic wave interference Well distanced from a machine generating a high frequency high voltage e g a centrifuge 10 2 MINIMAL OPERATION QUALITY Signal pro
7. DELAY 5s MAX UNITS 25 UNIT g l Method procedure MEASURE BLANK gt Insert measure zero solution NO ABS RESULT 1 0 675 19 8 Insert measure sample 2 0 843 24 8 Insert measure sample ROBERT RIELE GmbH amp Co KG Page 24 5 CALCULATION PROCEDURES 5010e 510 doc 16 01 08 5 4 2 Calculation procedure 2 C F Rb Method at which the difference of sample value As and reagent blank Ars is multiplied with a given factor F The reagent blank Ars is entered or measured once Calculation procedure eeee CP2 Characteristic 1 once a eh ak w sea ene cns C F Rb Method desi yan ront pereo ete ines End Point with Factor Calculation formula sssssssss C F As Ang o given entering Reagent blank sssssssssss entering or measuring Start method selection in the main menu PHOTOMETER 5010 5100 See chapter V5 10a 05 07 07 D 4 1 Measurement with programmed methods LAB RIELE GMBH CO KG 4 2 Measurement with basic methods DATE 07 10 07 TIME 08 52 24 In the case of activated printer the print out of the method data METHOD 21 HDL C follows PROGRAM 2 FACTOR 325 The measuring window is shown WAVELENGTH 546nm TEMPERATURE 37C MEAS VOLUME 900ul WASH VOLUME 1000ul DELAY 5s UNIT mg dl Method procedure MEASURE BLANK gt Insert measure zero solution Rb A 0 058 Insert measure reagent blank NO ABS RESULT i 1 i 1
8. LOADING PRINTER PAPER REPLACEMENT OF ASPIRATION TUBE When replacing the aspiration tube pay attention to the sequence of the assembly parts as shown below Then put aspiration tube without bending from the cuvette area side through the metal inlet tube Turn screw connec tion with fingers into the cuvette inlet tube with base screw connection milled O ring washer REPLACEMENT OF LINE FUSES The Photometer 5010 operates at any line voltage between 90 Vac and 264 Vac at 50 60 Hz without adjustment It has two line fuses in series with the power supply They are located on the rear panel To replace those fuses unplug the mains cable and remove the fuse holder with the fuses as shown below The instrument is delivered with two spare fuses A Neither use makeshift fuses nor short circuit the fuse holder Specifications of mains fuse e dimensions mm 5 20 e standard IEC 60127 2 V e time current characteristic time lag T voltage rating 250 V e rated current 1 6 A e marking T1 6AH mains switch fuse holder mains cable connector ROBERT RIELE GmbH 8 Co KG Page 63 9 ERROR MESSAGE CORRECTION 5010e 510 doc 16 01 08 9 1 9 2 9 3 9 4 ERROR MESSAGE CORRECTION GENERAL NOTE Faulty input e g wrong method number or wrong factor recognized by the user can be corrected by filling up the respective entry field with any signs After replenishing beyond the last position the faulty input
9. Measurement with programmed methods LAB RIELE GMBH CO KG 4 2 Measurement with basic methods DATE 07 13 07 TIME 08 45 09 In the case of activated printer the print out of the method data METHOD 26 SODIUM follows PROGRAM 6 STANDARD 150 0 The measuring window is shown WAVELENGTH 405nm TEMPERATURE 37C MEAS VOLUME 900ul WASH VOLUME 1000ul DELAY 3s MAX UNITS 300 UNIT mmol l Method procedure en MEASURE BLANK EE Insert measure zero solution Rb A 0 108 Insert measure reagent blank ST A 1 1 112 Insert measure standard 1 ST A 2 1 132 Insert measure standard 2 optional ST A 3 1 118 2 Insert measure standard 3 optional ST A 1 121 T Averaged standard FACTOR 148 2 Resulting factor NO ABS RESULT i 1 i 1 449 is 198 7 gt Insert measure sample das l dc 118 qe 149 6 i gt Insert measure sample 5 i 2 006 a 281 2 i gt Insert measure sample ROBERT RIELE GmbH 8 Co KG Page 29 5 CALCULATION PROCEDURES 5010e 510 doc 16 01 08 5 4 7 Calculation procedure 7 C S Sb Method at which the difference of sample value As and sample blank Asg regarding the amount is multiplied with a factor F which is determined by measuring of a standard solution with known concentration Cst and under consideration of standard blank Asrg The sample blank Asa is measured before every test Calculation procedure sss CP 7 Characteristics il C S Sb Method c
10. instability pollution expired reagents unfavorable delay time etc For a better monitoring the number of deltas deltas or At should be bigger than specified for the manual procedure The classic three minutes test with three deltas of 60 s can be replaced by 15 deltas of 12s When programming a new method which is based on CP 11 or CP 12 it is possible to set lower and upper limits for the measurement result within the method editor see chapter 6 METHOD EDITOR Fig 6 5 This can be achieved setting the parameters MIN VALUE and MAX VALUE If the measured value exceeds the MAX VALUE a message RANGE MAX is shown and if the measured value falls below MIN VALUE message RANGE MIN is shown Also a lower limit for R42 can be entered by setting MIN R 2 if the obtained R 2 value falls below the entered value a message RANGE R 2 is shown In order to get positive results at tests with decreasing absorbance see Fig 5 1 7 1 a negative factor has to be entered Only if MAX VALUE is set and the sign of the measured value is not equal to the sign of the entered MAX VALUE a message RANGE is shown The parameters MIN VALUE MAX VALUE and MIN R42 are deactivated entering a zero value ROBERT RIELE GmbH amp Co KG Page 20 5 CALCULATION PROCEDURES 5010e 510 doc 16 01 08 5 2 ABBREVIATIONS A ABS s Absorbance ARB iui Absorbance of reagent blank ARB Oi At Fixed Time absorbance of reagent blank after incubatio
11. 10 mm light path interchangeable with normal standard cuvettes macro or semi micro disposable or special optical glass Internal Peltier element temperature variable pre adjusted to 25 C 30 C and 37 C Equilibration time for aspirated reaction mixture to reach 37 C from ambient temperature 15 s Built in peristaltic pump driven by stepper motor programmable aspiration volume controlled by infrared light barrier Minimum 250 yl typically 500 ul up to 2000 ul Separate setting of aspirate volume and wash volume Touchscreen for direct functions and alphanumerical inputs Graphic display White characters or symbols blue background lighted resolution 240 128 dots Graphics printer dot matrix 24 characters per line English and German Indonesian Russian Spanish General operating software can be updated by PC Reagent open system with capacity for up to 231 programmable methods Data import by touchscreen or PC Up to 50 nonlinear calibration curves with max 20 sets of points can be stored Serial port for connection to an external printer and or PC Up to 2970 results of max 99 samples can be managed in the memory Absorbance End point with factor standard or multiple standards with or without reagent blank and or sample blank Bichromatic end point Kinetics with factor standard or multiple standards with or without blank Fixed time with factor standard or multiple standards with or without reagent
12. 20 diluted solution or ISE Cleaning Solution undiluted let it possibly affect up to 20 minutes 3 5 10 x rinse with dist water For device cleaning and surface decontamination purposes use commercial decontaminating solution which are usually available in clinical chemistry laboratories like Mikrozid AF Liquid Bacillol plus 3 Yo Kohrsolin or similar solutions Switch off the device and disconnect it from the mains voltage Then clean the device with soft cloth and decontaminating solution Empty the drain tank at the end of daily measurement or whenever filled CALIBRATING MEASURING SYSTEM At doubtful measurement results a dark level adjustment has to be carried out corresponding to chapter 7 2 1 ADJUSTMENT OF BUBBLE DETECTOR See chapter 7 2 4 Pump CALIBRATION OF PERISTALTIC PUMP See chapter 7 2 4 Pump REPLACEMENT OF RIBBON Open printer cover Lift used ribbon by pressing PUSH on the left Take ribbon cassette out of the printer Take new ribbon cassette out of the wrapping Turn small wheel on the left in the direction marked by an arrow till the inked ribbon is tightened Put in the ribbon cassette Pay attention that the paper is to be taken through inked ribbon and cassette Take printer paper through the crack of the printer cover and close the printer cover ROBERT RIELE GmbH amp Co KG Page 62 8 MAINTENANCE 5010e 510 doc 16 01 08 8 6 8 7 8 8 REPLACEMENT OF PAPER ROLL See chapter 2 4
13. 5 RIELE Operating Instructions Photometer 5010 vs ISS M ROBERT RIELE GmbH amp Co KG Software Version 5 10 Documentation Version 1 2008 5010e 510 doc 16 01 08 The packaging material the type plate on the instrument and the manual may contain the following symbols or abbreviations Manufactured by This product fulfills the requirements of Directive 98 79 EC on in vitro diagnostic medical devices In vitro diagnostic medical device IVD Caution refer to accompanying documents Please refer to safety related notes in the manual accompanying this device Please consult instructions for use Symbol for the marking of important information for appropriate handling of the device Description of the technical specifications according DIN 58 960 Teil 4 IP XO REF SN Biohazard Samples containing material of human origin must be treated as potentially infectious The relevant laboratory guidelines on safe use must be observed Symbol for the marking of electrical and electronics devices according to 7 ElektroG No special protection against penetrating moisture IP International Protection Order number Serial number The Photometer 5010 meets the requirement stated in Directive 98 79 EC of the European Parliament and the Council of the European Union EU on in vitro diagnostic medical devices Furthermore the Photometer 5010 is manufactured according to the special safety requirements for IVD m
14. DELAY 3s MAX UNITS 22 2 UNIT mmol l Method procedure uy MEASURE BLANK au gt Insert measure zero solution ST A 1 1 110 2 Insert measure standard 1 ST A 2 1 093 2 Insert measure standard 2 optional ST A 3 1 059 gt Insert measure standard 3 optional ST A 1 088 Averaged standard FACTOR 5 10 7 Resulting factor NO ABS RESULT ap 1 026 5 23 DUE gt Insert measure sample 2 l 1 357 l 6 02 a gt Insert measure sample 3 l 1 582 l 8 07 l gt Insert measure sample ROBERT RIELE GmbH 8 Co KG Page 28 5 CALCULATION PROCEDURES 5010e 510 doc 16 01 08 5 4 6 Calculation procedure 6 C S Rb Method at which the difference of sample value As and reagent blank Arg is multiplied with a factor F which is determined by measuring of a standard solution with known concentration Cst and under consideration of reagent blank Are The reagent blank Ars is entered or measured once Calculation procedure ssssssssssseeeeee CP6 Characteristic eti tiran ladan a Sena eat de eke ason C S Rb Method cocccccoccocococcconinacanononicnncnnanannoncos End Point with Standard Calculation formula esses C F As Ang Resulting factor sesssssssss F Cst Asr Ang Reagent blank ccecceseeeeeeeeeeeees entering or measuring Start method selection in the main menu PHOTOMETER 5010 5100 See chapter V5 10a 05 07 07 D 4 1
15. Kurf rstenstr 75 79 D 13467 Berlir Photometer 5010 cema TEES C ROBERT RIELE GmbH amp Co KG Page 11 3 OPERATING ELEMENTS 5010e 510 doc 16 01 08 3 3 TOUCHSCREEN MEASURE SAMPLE The touchscreen shows applications and information It is contact sensitive and reacts to the pressure exerted on it In order to execute a function the desired range on the screen must be touched A The surface of the touchscreen may be never touched with ball point pen pencil or another pointed article EI MODE WASH ZERO RESULT SP Sia BSC 06 15 06 3 3 4 WORKING AREA pump tube with joints REF 5010 050 outlet tube cuvette REF 5010 066 waste tubing REF 1704834001 aspiration tubing REF 5010 065 gt joint inlet tube cuvette REF 1707175001 ROBERT RIELE GmbH 8 Co KG Page 12 3 OPERATING ELEMENTS 5010e 510 doc 16 01 08 3 5 3 5 1 3 5 2 CUVETTES AND CUVETTE ADAPTOR Changing cuvette adaptor If a flow system is installed first the tubing system must be emptied Go to the main menu and execute a washing process by sipping lever P five times Air is aspirated and the residual liquid is pumped off through the waste tube Switch off the device If a flow system is installed disconnect the bubble detector remove the aspiration tube from the metal tube and disconnect the outlet tube cuvett
16. Page 7 CONTENTS 5010e 510 doc 16 01 08 1 INTRODUCTION TO PHOTOMETER 5010 This device is a semi automated programmable photometer for In Vitro Diagnostic IVD by qualified laboratory staff It is operated via touchscreen Remote control is possible by a serial data interface chapter 7 2 5 Serial interface REMOTE CONTROL For measuring methods several programmed methods with open parameters are available chapter 5 CALCULATION PROCEDURES and chapter 12 METHOD LIST Besides up to 231 methods built up on the basic methods can be established and stored by the operator with a method editor A list of methods can be printed out chapter 6 METHOD EDITOR Up to 50 nonlinear calibration curves with maximum 20 sets of points can be stored chapter 7 2 2 Multi standard functions Photometer 5070 offers a flexible cuvette concept A special flow through cuvette and a peristaltic pump provide for speedy operation with an optimal tempering of the measuring solution Alternatively the measuring solution can be measured in one way or glass cells in the provided standard cuvette adaptor By means of a combination of transistor and Peltierelement the solution reaches fast and accurately one of the three selectable temperatures 25 C 30 C or 37 C The Photometer 5070 is standard equipped with six optical filters of the wavelengths 340 405 492 546 578 and 623 nm If required they can be exchanged against any wavelength
17. a a lt s X TWA T tT Photodiode UT C uvette kod Cuvette adaptor Filter Glass for heat absorption ROBERT RIELE GmbH amp Co KG Page 71 10 TECHNICAL DATA 5010e 510 doc 16 01 08 Kindly contact directly the responsible dealer REF Description 5010 065 Aspiration tubing 185 mm 2 pcs 1706853001 Axis printer paper 1706870001 Bubble detector with plug 0573655001 Cuvettes of optical glass 4 pcs 501 002 Data cable serial interface 805 410 Disposable cuvettes 1000 pcs 1704818001 Dust cover 1704796001 Flow through cuvette adaptor 5010 018 Fuses for line power 10 pcs 0554871001 Halogen lamp 12V 20W 5010 024 Hitergent 1000 ml 0369624001 Interference filter 436nm 5010 670 Interference filter 670nm other wavelengths on request 500 002 Incubator T12 500 001 Incubator T16 1707175001 Joint inlet tube cuvette 0552402001 Mains cable 5010 005 Operators manual 5010 066 Outlet tube cuvette 5 pcs 1268457001 Printer paper 5 rolls 1010735001 Printer ribbon 1 pc 0745235001 Printer ribbon 5 pcs 5010 050 Pump tube with joints 090 064 Secondary calibration standards four piece certified 1704800001 Standard cuvette adaptor 090 063 Thermistor 1707574001 Top cover small for printer 1704834001 Waste tubing 2 pcs Incubator T12 Incubator T16 ROBERT RIELE GmbH amp Co KG Page 72 11 ACCESSORIES AND SPARE PARTS 5010e 510 doc 16 01 08 BASIC METHOD 15 basic methods chapter 12 1 free reserved for further 4 automatic calc
18. blank Turbidimetry with optional timer function Single double and triple determinations Curve fitting for nonlinear standard curves Free hemoglobin in combination with optional interference filters Up to 50 methods can be controlled with two control serums Levey Jennings plot Kinetic variable from 3 28 deltas time per delta 4 255 s Fixed time variable from 0 65535 s Programmable from 0 65535 s Range 90 Vac up to 264 Vac at 50 60 Hz Length 33 cm x width 34 cm x height 18 cm 6 kg CE Lv Page 68 10 TECHNICAL DATA 5010e 510 doc 16 01 08 10 5 CERTIFICATES EC Declaration of Conformity Manufacturer ROBERT RIELE GmbH 8 Co KG Address ROBERT RIELE GmbH amp Co KG Kurfuerstenstrasse 75 79 D 13467 Berlin Germany ROBERT RIELE GmbH amp Co KG declares under sole responsibility that the product Product name Photometer 5070 to which this declaration relates is in conformity with the following standards or other normative documents EN 61326 1 EN 61010 1 EN 61010 2 101 following the provisions of Directive 89 336 EWG EMC and 73 23 EWG LVD as well as 98 79 EG IVD This declaration describes an absorption photometer for laboratory use Berlin March 2006 ROBERT RIELE GmbH amp Co KG LU bt W Riele ROBERT RIELE GmbH amp Co KG Page 69 10 TECHNICAL DATA 5010e 510 doc 16 01 08 Technical Specifications Description according to DIN 58960 Teil 4 A Identi
19. factor F The reagent blank Arg is entered or measured once During the procedure the dialog asks for the use of a reagent blank The default value is OFF To continue with out reagent blank press ENTER After each measurement the next sample can be measured with NEXT With RESULT it is possible to measure the same sample again Calculation procedure sese CP 9 Characteristic eren FTK F Rb Method inei iere Fixed Time with Factor Calculation formula C F Aso As 1 ArB o Ang FACUON CL given entering Reagent blank entering or measuring UNIT MEASURE BLANK 3 0 829 DELTA A 0 3 2 1 029 1128 DELTA A 0 410 1381 2 DELTA A 0 502 PHOTOMETER 5010 5100 V5 10a 05 07 07 D LAB RIELE GMBH CO KG DATE 07 13 07 TIME 09 47 33 METHOD 29 CK MB PROGRAM 9 FACTOR 2751 3 WAVELENGTH 340nm TEMPERATURE 37C MEAS VOLUME 900ul WASH VOLUME 1000u1 INCUBATION 120s REACTION 180s MAX UNITS 1500 U L Rb A NO ABS RESULT 1 1 005 910 7 31 1 Start method selection in the main menu See chapter 4 1 Measurement with programmed methods 4 2 Measurement with basic methods In the case of activated printer the print out of the method data follows The measuring window is shown Method procedure gt Insert measure zero solution 0 000 Without reagent blank insert measure optionally gt Inse
20. is deleted and the entry field is free again for the renewed correct input Error messages by the device are carried out either exclusively via a signal tone chapter 9 2 ACOUSTIC ERROR MESSAGES or as combination signal tone and display In the display errors are shown as plaintext chapter 9 3 PLAINTEXT ERROR MESSAGES or coded with an error number chapter 9 4 CODED ERROR MESSAGES Each error message has always to be confirmed with E ACOUSTIC ERROR MESSAGES When pressing a key which is not permitted or not meaningful a deeper signal tone still sounds as error message after the higher signal tone which is to confirm the keystroke can be switched off according to chapter 7 2 14 Key signal ON OFF In the display no corresponding error message appears parallel to this The operation of the device can directly be continued by the correct keyboard entry PLAINTEXT ERROR MESSAGES RANGE MIN The programmed low limit was under run by the measurement RANGE MAX The programmed upper limit was exceeded by the measurement RANGE R42 The square of the correlation coefficient R lies at the kinetic measuring below the programmed low limit CODED ERROR MESSAGES method is write protected method cannot be cleared by special software check sum of a freely programmed method is wrong program new method 3 forbidden input wrong number format repeat input in permitted area method not available method editor check method no
21. memory for serum 2 EXIT Return to previous window ROBERT RIELE GmbH amp Co KG Page 52 7 UTILITY PROGRAMS 5010e 510 doc 16 01 08 MONTHLY MEMORY S METHOD 25 GLU SERUM 1 ET LOT G31 COMPANY LAB DATE MAY REQUIRED 14 4 RANGE La i 5 al CALCUL SS 1 012 m 13 860 1 07 09 07 EXIT MORE DELETE ROBERT RIELE GmbH amp Co KG SY Page 53 7 20 gt is mmol 1 T l 6 2 9 1 abnormal TECH 7 8 In the overview window of the selected method all data of quality control are visible In the line above the keys following information the current reading is indicated 1 Numerator of the monthly memory The oldest reading corresponds to the 1 01 27 06 gt Date of reading 13 30 Reading 1 s gt Deviation of the reading lies within minus 1s From 3s the warning level starts With a deviation of gt 3s an is displayed For the calculation of the quality values of a method at least 20 readings in the month memory must be present 1 User identification The keys have following functions Change to next reading Change to previous reading DEL Delete all measuring data of the monthly memory of the selected method and confirm again e g at change of serum MORE Change to output dialog EXIT Return to QC menu 7 UTILITY PROGRAMS 5010e 510 doc 16 01 08 PRN N PRN E EXIT Printout of the monthly memory of a method with seru
22. through cuvette For example this function makes it possible to pump two consecutive times 500 ul from a 1000 ul sample volume 7 UTILITY PROGRAMS 5010e 510 doc 16 01 08 MEDIA TEST 4 WATER ROBERT RIELE GmbH 8 Co KG Page 49 7 2 4 4 Media test With the media test the bubble detector can be checked and adjusted In the second line to the left of the medium WATER the current sensitivity level of the bubble detector is shown Sip distilled water by Sipping lever P Be sure that the aspiration tube is completely filled with water Enter new sensitivity level step by step by numeric key 0 up to 7 starting with O until the display changes from AIR to WATER Then increase the sensitivity level by one further step Empty the aspiration tube by Sipping lever P The display changes from WATER to AIR The adjustment is permanently saved by E 7 UTILITY PROGRAMS 5010e 510 doc 16 01 08 7 2 5 Serial interface A PC or an external printer can be connected to Photometer 5010 via the RS 232 serial interface at the back A suitable data cable can be supplied REF 501 002 The connected device must comply with safety standard EN 60950 The menu offers following functions e Activation and deactivation of EDP e Activation of remote control e Activation or deactivation of external printer with serial interface e Activation of download function of oper ating system 7 2 51 EDP ON
23. to Fig 6 3 METHOD 20 HEMOGLOBIN where the number of the method to be deleted F 29 4 405nm g dl is queried After a prompt for confirmation the selected method is deleted Basic and fixed METHOD 21 HDL C methods cannot be deleted E 2220000 denm MONAL LIST A list of all programmed methods can be printed and transmitted via the serial inter METHOD 30 CREATININ face S 2 0 492nm mg dl METHOD 31 GOT EXIT Return to main menu F 1746 0 340nm U l Fig 6 2 All methods from no 20 can be copied to a new method no First the method to be copied is queried in Fig 6 3 Its parameters METHOD COPY can be changed starting with Fig 64 LAST The method used last can be copied on a new method place Its setting parameters can be changed starting with Fig 6 4 EXIT LAST H gt H This function is very useful if a basic method with new setting parameters was successfully tested These parameters can be saved as a new method starting from no 20 EXIT Return to main menu ROBERT RIELE GmbH 8 Co KG Page 40 6 METHOD EDITOR 5010e 510 doc 16 01 08 Fig 6 3 METHOD NO METHOD NAME UNIT DIM 132 3 4 5 6 ESC 0 Fig 6 4 parameter window 1 ETHOD EDIT RV x CONOUBWNE vor od DO OS ch Ni IAOZCONME Himrmzmrm rm Hm mrzEoOoc m Uo 322 uv 3m NP mor ga 22m mH Dz rzo AND Hor cA Bcc API U x mz orm c m gt DH z no i 42 H Fig 6 5 parameter window 2 D EDIT m WISIIZ
24. within the range of 340 800 nm Three additional filters e g 670 nm can be installed The device is equipped with a rugged graphics printer 8 dot matrix with 24 characters per line using standard paper The measuring data can be stored and managed in the Photometer 5070 chapter 7 2 8 Data logging According to a GLP conformal documentation the names of lab and operator can be printed out as well as transferred to EDP chapter 7 2 5 Serial interface EDP ON OFF In Photometer 5070 up to 50 methods can be supervised with a quality control chapter 7 2 6 Quality control Numerous utility programs permit the individual configuration of the device Function tests support the analysis of sources of error Photometer 5070 is future proof by FLASH MEMORY technology The operating system can be updated with program novelties and or improvements comfortably without having to open the equipment chapter 7 2 5 Serial interface DOWNLOAD ROBERT RIELE GmbH amp Co KG Page 8 1 INTRODUCTION TO PHOTOMETER 5010 5010e 510 doc 16 01 08 2 1 DELIVERY Check the device and contents of the enclosed box as follows on visible transport damages and completeness Operator s Manual Aspiration tube Axis printer paper Dust cover Fuses for line power Mains cable Printer paper Pump tube with joints Ribbon band printer Standard cuvette adaptor Top cover small for printer Waste tube A P Y A Inform th
25. 010 5100 DATE 02 03 06 5 3 11 2 14 2 35 3 ROBERT RIELE GmbH amp Co KG Page 56 7 UTILITY PROGRAMS 5010e 510 doc 16 01 08 7 2 9 Temperature ON OFF 7 2 10 Temperature adjustment PERATURE D SELECT TEMP ADJUSTMENT TEMP ACTUAL ROBERT RIELE GmbH amp Co KG 36 90 Page 57 The current status of the tempering is indi cated in the first line by OFF or ON Besides the inserted cuvette adaptor is marked by D or E Change setting by SELECT Following options are possible e OFF e 25 C e 30 C e 37 C Save setting permanently by OK Save setting temporarily up to next switch off of the device by EXIT A The temperature control was adjusted at the factory Anyhow the tempering control can be cali brated provided that the tempering was switched on for at least 30 minutes Measure the current temperature with an independent measuring system e g thermis tor REF 090 063 inside the cuvette and enter this value According to the difference to 25 0 C 30 0 C or 37 0 C the system corrects its internal setting The calibrating of the tem perature is interrupted when the tempering is off or the temperature unstable Enter the password 5010 Enter the actual temperature in TEMP ACTUAL four digit in C e g 36 90 and confirm by E 7 UTILITY PROGRAMS 5010e 510 doc 16 01 08 7 2 11 Laboratory name p p epe pm p SS pm pL an D P P
26. 064 327 uu Insert measure sample i 1 188 i 367 ui 2 Insert measure sample 3 i 1 340 i an n gt Insert measure sample ROBERT RIELE GmbH 8 Co KG Page 25 5 CALCULATION PROCEDURES 5010e 510 doc 16 01 08 5 4 3 Calculation procedure 3 C F Sb Method at which the difference of sample value As and sample blank Asg regarding the amount is multiplied with a given factor F The sample blank Asg is measured before every test Calculation procedure eee CP 3 Characteristic c reticere redes C F Sb Method eter preteen anpe End Point with Factor Calculation formula sssssssse C F As Ase dz E E given entering Start method selection in the main menu PHOTOMETER 5010 5100 See chapter V5 10a 05 07 07 D 4 1 Measurement with programmed methods LAB RIELE GMBH CO KG 4 2 Measurement with basic methods DATE 07 09 07 TIME 09 45 32 In the case of activated printer the print out of the method data METHOD 23 BILIRUBIN follows PROGRAM 3 FACTOR 12 80 The measuring window is shown WAVELENGTH 546nm TEMPERATURE 37C MEAS VOLUME 900ul WASH VOLUME 1000ul DELAY 5s MAX UNITS 8 0 UNIT mg dl Method procedure MEASURE BLANK gt Insert measure zero solution NO ABS RESULT 1 1 000 4 21 2 Insert measure sample blank Sb A 0 671 gt Insert measure sample 2 1 215 4 25 gt Insert measure sample blank Sb A 0 884 2 Insert measu
27. 07 14 52 QC EDP not implemented 7 2 6 1 INPUT OF CONTROL SERUM CONTROL SERUM 1 iu 1 Enter name max 15 digit 1 ID En Pee 3 Enter LOT no max 10 digit 4 2 COMPANY 0K 5 Enter company max 10 digit 8 3 SE Enter expiry date max 8 digit NO Change to next control serum EXIT and OK Accept input and return to 37 00C 07 13 07 14 52 QC menu ROBERT RIELE GmbH amp Co KG Page 51 7 UTILITY PROGRAMS 5010e 510 doc 16 01 08 7 2 6 DAILY MEMORY S1 Select daily memory for serum 1 S2 Select daily memory for serum 2 EXIT Return to previous window Measuring data of the corresponding daily memory are shown with method number method name reading and dimension Change to next reading Change to previous reading DEL Delete shown reading in the daily memory and confirm again by DEL STORE Store shown reading in the daily memory and confirm again by STORE Afterwards the reading is deleted in the daily memory Print out of dail f 1 i HD out 9r cary memory tor senum l PRINT Print all readings DAILY MEMORY S1 PHOTOMETER 5010 5100 V5 10a 05 07 07 D LAB RIELE GMBH CO KG EXIT Retum to QC menu DATE 07 10 07 TIME 08 30 03 25 GLUCOSE 13 03 21 HDL C 367 27 UREA COL 197 2 29 CK MB 1128 1 31 GOT 189 9 7 2 6 3 MONTHLY MEMORY After query of method number select serum 1 or 2 of the method S1 Select monthly memory for serum 1 S2 Select monthly
28. B 3 Specified measuring range Outside the specified measuring range and under rated operating conditions other than those stated in section B 4 the maximum permissible errors given in section B 5 can be exceeded B 3 1 Spectral absorbance A A 0Ato25A B 3 2 Wavelength A useable for measurements 340 nm to 800 nm B 4 Specified Operation conditions B 4 1 Spectral transmittance of the cuvette 75 Yo B 4 2 Warm up time 15 min B 4 3 Operating voltage mains between 90 Vac and 264 Vac at 50 60 Hz B 4 4 Ambient temperature 15 C to 35 C B 4 5 Sound pressure level SPL lt 50 dB B 5 Maximum permissible errors and other limiting values B 5 1 Photometric uncertainty of the spectral absorbance max 3 96 B 5 2 Photometric short time variation coefficient 1 Yo B 5 3 Uncertainty of wavelengths max 2 nm B 5 4 Spectral half width of spectral radiation flux at detector lt 10nm B 5 5 Percentage of wavelength integrated false radiation measured at 340 nm as transmittance of a cut off filter NaNOs 0 1 Yo B 5 6 Uncertainty of temperature regulation lt 0 2 C B 5 7 Specimen related carry over ratio measure according to Broughton with p Nitro phenol lt 3 OPTIC CONSTRUCTION Am path of rays is directed from the back to the front of the device Insert standard cuvette accordingly Diaphragm 1 Diaphragm 2 Diaphragm 3 Diaphragm 4 Lens 2 Lens 3 4 MODE d Halogen lamp Y NS
29. DELTA Endpoint with Factor C F AAg2 sb2 AAsi sbi CP 15 C F 3 WL Measurement with 3 Wavelengths C 168 Agisnm 84 Azgonm 84 Aasonm Explanations CP NO entien Number of the calculation procedure chapter 6 METHOD EDITOR Characteristic Name of the calculation procedure chapter 12 1 BASIC METHOD Calculation formula Calculation basis of basic method ROBERT RIELE GmbH amp Co KG Page 22 5 CALCULATION PROCEDURES 5010e 510 doc 16 01 08 5 4 DESCRIPTION OF METHOD PROCEDURES In the descriptions of the calculation procedures a typical print out by the internal printer is shown on the left side All examples were built with active pump In case of measurements without pump the volumes for sipping the media are not printed All print outs begin with the device information laboratory data and method parameters followed by all measur ing data necessary for a manual examination of the readings The measuring window The arrangement of the measuring window is alike in all calculation procedures Depending on the method various numbers of readings or diagrams are shown status of measuring system or next action MEASURE SAMPLE e 16 5 n reading window numerator dimension action keys MODE WASH nn info line Functions of the action keys in the measuring window Oa dS 5 name of active method Glucose ZERO RESULT 07 1925 8424 R
30. E2 E1 is measured several times depending on the quantity of samples In the first course the samples E1 maximum 25 will be measured optionally with or without sample blank After a user defined measure time the samples E2 will be measured in a second course Attention should be paid to the order within the series to avoid errors The procedure corresponds to a fixed time kinetic and is normally processed using the standard cuvette adaptor Quality control samples can not be saved This calculation procedure has special parameters that allow a time controlled measuring process These parameters are time delta T1 measure time T2 delay T3 reagent time 2 and reagent time 3 By setting a time delta value between 10s and 255s the other parameters for the time controlled measuring will be used In the time controlled mode the quantity of samples is determined by the measure time and the time delta e g with a measure time of 60s and a time delta of 10s it is possible to measure 6 samples without sample blank The measure time should be chosen greater than or equal to the time delta At the beginning of the method the use of a sample blank is queried After the blank is measured the time controlled measuring process will be started with RESULT With a combination of acoustic signals and text messages on the display the Photometer takes control of the timing for the whole measuring The measurement of the samples E1 can be finished at any ti
31. GE UTILITIES LAMP 37 05C OY 6 dksh 5 7 METHODS After around 15 minutes the device is heated up and ready for measurement First the tempering is switched off If working with tempered material is required later switch on the tempering already now either directly by the utility program chapter 7 2 9 Temperature ON OFF or indirectly by selec tion of a method with programmed tempering chapter 5 1 GENERAL NOTES If errors appeared during operation first of all they have to be confirmed with E before remedy chapter 9 ERROR MESSAGE CORRECTION 2 4 LOADING PRINTER PAPER With initial operation or if the colored end of the paper roll appears printer paper must be inserted e Open the printer cover and remove the rest of paper e Put printer paper axis into the new printer paper reel e Fold the beginning of paper over the corner to an angle of 90 as shown below e Insert the bent paper underneath the ribbon cartridge e Press LF several times for line feed until the paper has a length of about 5 cm e Insert printer paper reel into the axis guide e Push the printer paper through the slot in the printer cover and close the printer with the cover ROBERT RIELE GmbH amp Co KG Page 10 2 INSTALLATION 5010e 510 doc 16 01 08 3 1 FRONT printer cover lid working area touchscreen aspiration tube sipping lever P 3 2 BACK n ROBERT RIELE GmbH amp Co KG y M
32. GUAGE GERMAN Save setting permanently by OK The setting is temporarily saved up to next switch off of the device by END 7 UTILITY PROGRAMS 5010e 510 doc 16 01 08 7 2 18 ADC counts Optic p EM a a ee pj ld bad sid ler le sal lal id Ead i ail lesa pu pe pu a pg pa pr pH o koi koi koi oi kai Hiei A en E aa aa aaa aaa azi aaa kaa aaa aaa 7 2 19 Service tools ROBERT RIELE GmbH amp Co KG Page 61 Indicated is the current value of the optical analogue digital converter The value is proportional to the light current depending on the selected amplification level and the boost setting To a key actuation the system reacts possibly only after three seconds The functions VO to V7 set the amplification level The function BO increases boost ON or reduces boost OFF the time slot The functions FO to F9 place the filter wheel into the positions 0 to 9 The position 0 corre sponds to the filter wheel position at the dark level adjustment Stop function by ESC The service tools are reserved for trained specialists only and therefore protected by a password Stop function by ESC 7 UTILITY PROGRAMS 5010e 510 doc 16 01 08 8 8 1 8 2 8 3 8 4 8 5 MAINTENANCE This chapter provides necessary information concerning general maintenance by the user A If any faults should occur which cannot be remedied then service should be contacted Repairs at th
33. H 546nm TEMPERATURE 37C MEAS VOLUME 900ul WASH VOLUME 1000ul DELAY 3s MAX UNITS 12 Method procedure gt Insert measure zero solution Rb A 0 150 gt Insert measure blank of reagent blank Rbb A 0 046 gt Insert measure reagent blank DELTA Rb 0 104 71 Resulting blank gt Insert measure standard blank ST A 1 1 485 gt Insert measure standard 1 ST A 2 1 521 gt Insert measure standard 2 optional ST A 3 1 495 Insert measure standard 3 optional ST A 1 501 Averaged standard STb A 0 479 1 standard blank DELTA ST 1 022 T Averaged standard minus standard blank FACTOR 8 i 74 4 Resulting factor E EE da gt Insert measure sample blank Sb A 0 489 Insert measure sample en 2 1 542 7 7 89 m gt Insert measure sample blank Sb A 0 535 gt Insert measure sample CI a e no kt gt Insert measure sample blank 3 1 394 8 39 gt Insert measure sample Sb A 0 329 ROBERT RIELE GmbH amp Co KG Page 31 5 CALCULATION PROCEDURES 5010e 510 doc 16 01 08 5 4 9 Calculation procedure 9 FTK F Rb Method at which a reagent blank is measured after an incubation time Ak and after a reaction time gt Arg and also a sample after an incubation time As o and after a reaction time gt As 1 The difference from the change of the test and the change of the reagent blank is multiplied by a predefined
34. OBERT RIELE GmbH amp Co KG EXIT Leads to the query whether the measuring program is to be terminated MODE Occupies the action keys with following mode functions NUM MODE LF QC RETURN PRN DETAIL LAMP M STD RETURN WASH With activated pump the wash volume defined to the method is pumped ZERO Starts the zero measuring With activated pump the supping lever P must also be pressed RESULT Starts the measuring With activated pump a solution already sipped is measured once again To aspirate and measure a new sample press sipping lever P Page 23 5 CALCULATION PROCEDURES 5010e 510 doc 16 01 08 5 4 4 Calculation procedure 1 C F Method at which a measured sample value As is multiplied with a predefined factor F Calculation procedure CharacteriStiC occooocononnccnccncocononnnonnononononononanencnnonaronnan Method e lia Calculation formula ooooocccccccccononnconnnnnonononannnnonos Facto AAEE EA AAEE EET EA A E Start method selection in the main menu PHOTOMETER 5010 5100 See chapter V5 10a 05 07 07 D 4 1 Measurement with programmed methods LAB RIELE GMBH CO KG 4 2 Measurement with basic methods DATE 07 10 07 TIME 08 44 12 In the case of activated printer the print out of the method data METHOD 20 HEMOGLOBIN follows PROGRAM 1 FACTOR 29 4 The measuring window is shown WAVELENGTH 405nm TEMPERATURE 37C MEAS VOLUME 900ul WASH VOLUME 1000ul
35. OFF The current status of the EDP Electronic Data Processing interface is indicated in the first line by OFF or ON Change setting by SELECT Following options are possible e EDP OFF e EDP ON CR LF e EDP ON STX ETX BCC Save setting permanently by OK Save setting temporarily up to next switch off of the device by EXIT 7 2 5 2 REMOTE CONTROL Activate remote control by START When activated Photometer 5010 can be remote controlled by a PC and a suitable program Deactivate remote control by sipping lever P By EXIT the program returns to the superordinate menu item 7 2 5 3 _ SERIAL PRINTER ON OFF The current status of the external serial printer is indicated in the first line by OFF or ON Change setting by SELECT Save setting permanently by OK Save setting temporarily up to next switch off of the device by EXIT ROBERT RIELE GmbH amp Co KG Page 50 7 UTILITY PROGRAMS 5010e 510 doc 16 01 08 7 2 6 7 2 5 4 DOWNLOAD The operating system of Photometer 5010 is contained in an electronically erasable and programmable FLASH MEMORY With the download function the operating system of the Photometer 5010 can be updated by PC DOWNLOAD PLEASE WAIT The download function is controlled by an appropriate software and terminated after actualization A break of the function cannot be accom plished by key Only switching the photometer off terminates the download function Quality con
36. RE BLANK 2 Insert measure zero solution Rb A 0 085 gt Insert measure blank of reagent blank Rbb A 0 198 gt Insert measure reagent blank DELTA Rb 0 113 Resulting blank NO ABS RESULT 1 0 715 154 gt Insert measure sample blank Sb A 0 486 gt Insert measure sample gt 0 646 49 gt Insert measure sample blank Sb A 0 497 gt Insert measure sample ROBERT RIELE GmbH amp Co KG Page 27 5 CALCULATION PROCEDURES 5010e 510 doc 16 01 08 5 4 5 Calculation procedure 5 C S Method at which a measured absorbance value As is multiplied with a factor F which is determined by measur ing of a standard solution with known concentration Csr Calculation procedure 00 0 eeeceeeeeeeeeeenneeeseeneeeeenneeeenees CP 5 Characteristic rn pese orina C S Method tito iei cene tere End Point with Standard Calculation formula sssssssssssss C F As Resulting factor cccccceeeeeeeeeceeeeeeeeeeeeeneeeaees F Csr Asr Start method selection in the main menu PHOTOMETER 5010 5100 See chapter V5 10a 05 07 07 D 4 1 Measurement with programmed methods LAB RIELE GMBH CO KG 4 2 Measurement with basic methods DATE 07 13 07 TIME 08 33 42 In the case of activated printer the print out of the method data METHOD 25 GLUCOSE follows PROGRAM 5 STANDARD 5 55 The measuring window is shown WAVELENGTH 546nm TEMPERATURE 37C MEAS VOLUME 900ul WASH VOLUME 1000ul
37. ZEZ d RV x lt UNXD gt zvoz rr ONOUBWNHE Z it bet oo oO HED gt EMHAH gt A IT O rMnra2x lt x2 o DH HO lt lt PaANMZNncc 4 gt cr gt mm HZZONIZ aomam gt D 2 m o YA Fig 6 6 parameter window 3 METHOD EDIT RV x Ll Dae SAL 2 MIN VALUE S1 3 REQUIRED SI 4 MAX VALUE S1 Bebb SZ 6 MIN VALUE S2 7 REQUIRED S2 8 MAX VALUE S2 ROBERT RIELE GmbH amp Co KG 7 8 E NAME EXIT 0K ON 8 P1 3 NAME EXIT OK 8 P2 3 NAME EXIT 0K 8 P3 3 Page 41 Inquiry window of the desired method The method used last is suggested With or the methods can be scrolled A numeric input of the method number is possible at any time A known method is indicated with name and dimension E Select shown method ESC Return to main menu The parameter windows 1 and 2 show the general method data The parameter window 3 has special functions which are necessary for quality control only see below For each setting parameter a leading identifi cation number is shown If the identification number is selected on the keyboard the cor responding setting parameter becomes configurable Number and kind of setting parameters depend on the calculation procedure So identification numbers can be occupied variedly Characteristic numbers without parameters do not have a function EXIT Return to main menu OK Accept setting parameters depending on editor mode sometimes with query of target metho
38. ase of activated printer the print out of the method data METHOD 15 C F 3 WL follows PROGRAM 15 FACTOR 1 00 The measuring window is shown WAVELENGTH 380 415 450nm TEMPERATURE 37C DELAY 2s UNIT mg dl MEASURE BLANK gt Insert measure zero solution NO umol L mg dl gt The result will be displayed in two units of measurement 1 4 859 7 83 MUS MEN uia Nov demie Lacu tap ro gt Insert measure sample 2 5 865 9 45 ROBERT RIELE GmbH amp Co KG Page 39 5 CALCULATION PROCEDURES 5010e 510 doc 16 01 08 6 METHOD EDITOR By the method editor the daily laboratory work can be substantially facilitated Based on the 15 calculation procedures up to 231 user defined methods with their setting parameters can be saved With the functions of the editor a method can be established changed or deleted Fig 6 1 METHOD NEW CHANGE COPY In the main window of the method editor following options are available METHOD COPY EES METHOD COPY Change to Fig 6 2 where different copy functions can be selected METHOD EDIT METH EDIT Change to Fig 6 3 where the number of the method to be edited is queried METHOD NEW Afterwards all setting parameters of the selected method can be changed METHOD DELETE EXIT METHOD NEW Change to selection of the 37 05C 07 13 07 12 88 calculation procedure see 5 3 SURVEY OF THE METHODS In Fig 6 4 all setting parameters can be edited Printout of a method list METHODE DELETE Change
39. asuring solution El E comunican mentioned module does not answer ina check interconnecting cable certain time check mentioned module communication overflow send receive buffer reduce amount of data at the communication partner a EAN Dann n a e o ROBERT RIELE GmbH amp Co KG Page 65 9 ERROR MESSAGE CORRECTION 5010e 510 doc 16 01 08 error at pump calibration clamp pump tube control volume of aspiration control connectors of tubes the plug of the bubble detector has a bad contact pump tube not clamped no liquid aspirated too much liquid aspirated during calibrating power of the pump too small the cable of the bubble detector has a motor does not rotate or stops bad contact connectors of tubes leaky adjust bubble detector chapter 7 2 4 bubble detector works falsely 7 a aspiration tube blocked replace aspiration tube chapter 8 7 aspiration tube badly stained error at sipping solution clamp pump tube adjust bubble detector chapter 7 2 4 le control volume of aspiration no liquid aspirated control connectors of tubes 3 Ponera the ra kan kran do a calibration of peristaltic pump motor does not rotate or stops chapter 7 2 4 connectors of tubes leaky bubble detector works falsely aspiration tube blocked aspiration tube badly stained pump tube not clamped the plug of the bubble detector has a bad contact
40. ater or another appropriate rinse solution by pressing WASH or sipping lever P This is also neces sary after a method change chapter 8 1 CLEANING INSTRUCTION For the procedure within a measuring series see the application regulation In order to aspirate solution into the measuring system put the aspiration tube deeply enough into the respective vessel Before setting to zero press ZERO Trigger setting to zero by sipping lever P Trigger a normal measuring by sipping lever P Repeat a measuring of a solution which is already aspirated by RESULT To work in optimized volume mode use the function Volume optimized ON OFF chapter 7 2 4 3 2 This function makes it possible for example to pump two consecutive times 500 ul from 1000 ul sample volume ROBERT RIELE GmbH amp Co KG Page 13 3 OPERATING ELEMENTS 5010e 510 doc 16 01 08 3 5 3 Working with standard cuvettes Am path of rays is directed from the back to the front of the device Insert single cuvette according to the drawing OPTIC CONSTRUCTION in TECHNICAL DATA Trigger setting to zero by ZERO Trigger a normal measuring by RESULT 3 5 4 Working with discrete flow through cuvette For use of a discrete flow through cuvette insert the standard cuvette adaptor Am path of rays is directed from the back to the front of the device Insert flow through cuvette according to the drawing OPTIC CONSTRUCTION in TECHNICAL DATA Pay attention to the correct con
41. aximum 25 Results based on the differences of the measured samples gt Show the results by MODE MODE DETAIL Method procedure without sample blank Measure all samples E1 maximum 25 Change to measuring E2 by E1 E2 gt Measure all samples E2 maximum 25 Results based on the differences of the measured samples 3 Show the results by MODE MODE DETAIL ROBERT RIELE GmbH amp Co KG Page 38 5 CALCULATION PROCEDURES 5010e 510 doc 16 01 08 5 4 15 Calculation Procedure 15 C F 3 WL Method at which a sample is measured with three different wavelengths 380 nm 415 nm and 450 nm This method is appropiated for free hemoglobin measurements The mentioned wavelengths are not included in the standard set of filters The factor has to be adjusted if using thinner see chapter 5 1 3 Calculation procedure iesti i e eae eE a eae CP 15 Characteristic kwa ak aaa en pd cer cdi C F3WL Method iiiter ettet Measurment with 3 wavelengths Calculation formula C mg dl F 168 A415nm 84 Aasonm 84 Ausonm Factor ii pec e ete e te a ope ieee ve Pede given entering Conversion factor eiecit ei petere eere iine umol L 0 6206 mg dl Start method selection in the main menu PHOTOMETER 5010 5100 See chapter V5 10a 05 07 07 D 4 1 Measurement with programmed methods LAB RIELE GMBH CO KG 4 2 Measurement with basic methods DATE 07 10 07 TIME 08 16 31 In the c
42. cessing in analogue amplifiers with high amplification factors cannot differentiate desired from undesired signals Amplifiers thus are apt to be overloaded or produce spurious signals The equipment will operate correctly when the undesired signals are removed Short term changes of the operational behavior do not influence the overall function of the device 10 3 TYPE PLATE For installation pay attention to the specifications on the type plate ROBERT RIELE GmbH Co KG Kurf rstenstr 75 79 D 13467 Berlin Made in Germany Photometer 5010 90 264 V 50 60 Hz 130 VA max Fuse T 1 6 A H 250 V ROBERT RIELE GmbH amp Co KG Page 67 10 TECHNICAL DATA 5010e 510 doc 16 01 08 10 4 SHORT SPECIFICATIONS Type Light Source Wavelength Wavelength Selection Photometric Range Cuvette System Temperature Control Aspiration System Sipping Volume Operator Interface Data Presentation Integrated Printer Languages Memory Signal Port Data Logging Measurement Procedures Quality Control Measuring Time Delay Time Mains Supply Dimensions Weight Marking ROBERT RIELE GmbH 8 Co KG Semi automatic single beam filter photometer Halogen lamp 12 V 20 W with protection for lifetime 340 nm 800 nm Automatic via 9 position filter wheel 6 standard interference filters 340 nm 405 nm 492 nm 546 nm 578 nm and 623 nm 3 positions for optional filter of choice 0 2 5A Micro flow cell 32 ul
43. ction of the aspiration tube can be seen confirm the event by OK During the second phase the system pumps 1000 ul distilled water by Sipping lever P repeatedly The turn of the pump wheel is shown beside the entry WATER As soon as the end of the liquid in the tube between the metal inlet tube and the screw connection of the aspiration tube can be seen confirm the event by OK Save setting permanently by OK 7 2 4 3 Pump settings In this menu it is possible to configure the bubble detector and the optimized volume mode After configuring the bubble detector the window for the configuration of the optimized volume mode will be displayed automatically 7 2 4 3 1 Bubble detector ON OFF The current status of the bubble detector is indicated in the first line by OFF or ON Change setting by SELECT Save setting permanently by OK Save setting temporarily up to next switch off of the device by EXIT 7 2 4 8 2 Volume optimized ON OFF The current status of this setting is indicated in the first line by OFF or ON Change setting by SELECT Save setting permanently by OK Save setting temporarily up to next switch off of the device by EXIT When volume optimized is on an acoustic signal will be emited after the required volume is pumped At this moment the sample must be retired from the aspiration tube After a pause of approx 1s the rest of liquid in the tube will be completely pumped into the flow
44. d S 3 Change to next parameter window Specifics in parameter window 3 At least one control serum must be defined before data can be entered see chapter 7 2 6 Quality control If at least one control serum with its setpoint and range is entered corresponding memory of the quality control is reserved for this method So it can be supervised with inte Fi quality control If both ID identifications are deleted then also all data and reserved memory of this method in the quality control are deleted 6 METHOD EDITOR 5010e 510 doc 16 01 08 ROBERT RIELE GmbH amp Co KG Page 42 Query of the desired method number under which the new method is to be stored The next free method number is indicated How ever each free method number can be selected within the range of 20 to 250 E Store method with selected number In case of multi standard method Fig 6 8 follows ESC Break storage and return to editor menu For a method with multi standard there is the editor window for the curve bases P and P Consecutive numbering of the current bases A C Switch input between A for absorbance and C for concentration E Accept the edited value Li Input and confirmation of a single 0 at A lead to the deletion of the current pair of points In order to set the value to zero enter e g 0 0 Fi End input and save curve data For measuring in a multi standard method at least 2 bases wi
45. d point measurement CONC W FACTOR RB e Fixed time kinetic two point kinetic CONC W FACTOR SB e Kinetic CONC W FACTOR RB SB EXIT e Transmission Scrolling through all methods is possible by PAGE The current page is shown at the right upper screen corner By END the program returns to the main menu A method is selected by pressing the corre sponding key The following abbreviations are used for the distinction of the methods CONC concentration KIN kinetic F factor STD standard RB reagent blank SB sample blank Further information Chapter 5 CALCULATION PROCEDURES 4 3 Method editor METHOD NEW CHANGE COPY Each photometric test can be permanently stored with its setting parameters by the method editor METHOD COPY LIST With the functions of the method editor are METHOD EDIT possible the new installation the change and removing a method METHOD NEW By LIST an overview of the programmed methods can be printed and transmitted via METHOD DELETE EXIT the serial interface 37 05C O7 le OU 12 53 Further information Chapter 6 METHOD EDITOR ROBERT RIELE GmbH 8 Co KG Page 16 4 PROGRAM SELECTION 5010e 510 doc 16 01 08 ROBERT RIELE GmbH amp Co KG Page 17 Utility programs are necessary for the adjust ment and maintenance of Photometers 5010 Further information Chapter 7 UTILITY PROGRAMS By LAMP in the main menu the halogen lamp can be switched off temporar
46. differentiation between normal and abnormal activities This is followed by a sequence of measurements in regular time intervals Deltas or At An example of a resulting curve is shown in Fig 5 1 7 1 0 85 Kinetic ABSA 0 8 0 75 ABS 2 or 0 65 pr 06 pen 0 55 o Aaa cu Ww AE SM Delay time g At p At ye At q NA At p 045 04 i i i f i i 0 12 24 36 48 60 tis 72 84 96 108 120 132 Fig 5 1 7 1 Resulting curve of kinetic test decreasing absorbance In each time interval Delta or At the difference between the relating absorbance values as well as the gradient of the curve are calculated To obtain the alteration per minute AAs minute the gradients must be averaged This is done by a simple linear regression calculation also giving an indicator for the linearity of the test This indicator is called the coefficient of correlation R For practical reasons the square of the coefficient of correlation R 2 is taken in a Kinetic calculation The value of R42 can vary between 0 and 1 An R42 value of 1 indicates perfect linearity and a value of 0 indicates absolute non linearity Already values lt 0 9 indicate a bad linearity and therefore an incorrect test In practice linear tests show values of R 2 near to 1 In the example for Calculation procedure 11 KIN F Rb values of R42 2 0 998 are permitted Results with smaller R42 values could be caused by temperature
47. e e iicet aa ar 18 5 1 4 Fundamental to the methods with standard sssssee ee 19 5 1 5 Fundamental to the methods with multi standards ooooncccnnoccconoccccnononnnnnona nano nonnncnnno cnn nnnnn no 19 5 1 6 Fundamental to bichromatic measurements ssssssse ee eere 19 54 7 Fundamental to the Kirietlc eui id 20 5 2 ABBREVIATIONS ciii eire kie E vno pak Enea ed ie LR de esto Fo sra ove et E da vat Eo sare dete na 21 2 3 SURVEY OF THE METHODS cut e tede D diese dete en E RE vise pi pede Rte 22 5 4 DESCRIPTION OF METHOD PROCEDURES eene eren eren nere enne nnns 23 5 4 1 Calculation procedure 1 iPod aene D ote ae es 24 5 4 2 Calculation procedure 2 C F Rb ssesssseee eene eene enne ener enne nnns 25 5 4 3 Calculation procedure 3 C F SD ssssseeee eene nennen enne 26 5 4 4 Calculation procedure 4 C F SDRD ooocconoccconococcnononcncnononcnonono nono non nnnnnnn eem nennen nennen nnns 27 5 4 5 Calculation procedure 5 C S miniin oi a oeira aiai nnne enne nns 28 5 4 6 Calculation procedure 6 CIS RD oooonccccnocicinococcnononcncnononcnn nono nonn nn n nr rana nnnm nennen nnne 29 5 4 7 Calculation procedure 7 C S Sb vivise iita eieiei ieiti renea kaeida daiiran 30 5 4 8 Calculation procedure 8 C S SbRb ssssseseeeeeeneene eene nee nennen 31 5 4 9 Calculation procedure 9 FTK F RD oooccccnocccnnococcnononcncnononcn
48. e device may be carried out only by authorized specialist staff Through improper repairs the warranty extin guishes and the user can be heavily jeopardized CLEANING INSTRUCTION A Liquid waste is potentially biologically hazardous Always wear gloves if handling those materials Do not touch parts of the device other than those specified Consult the laboratory protocol for handling biohazardous materials d care that no liquid enters the device There is no protection against penetrating of liquids Code IP X0 Check from time to time that the tubing and the connections are leakproof The flow through system of the Photometer 5070 has to be washed with dist water regularly before and after measuring 2 3 times Depending on sample material and reagent and always at the end of a working day the Photometer 5010 has to be cleaned additionally with a phosphate free detergents e g with approx 5ml of 1 Yo Hitergent solution REF 5010 024 and afterwards rinsed with 5ml distilled water Persistent residues are to be removed with a combined alkaline acid treatment The following procedure is advised step by step 1 5x wash with NaOH 1 N 2 5 x rinse with dist water 3 5x wash with HCI 0 5 N 4 5 xrinse with dist water If the system is contaminated severely it may alternatively be cleaned with hypochlorite The following proce dure is advised step by step 1 5 x rinse with dist water 2 2 3 x wash with hypochlorite 1
49. e display changes between C und XX XXC In the case of switched on tempering and instable temperature the display changes between C and e g 37 03C In the case of stable temperature the current temperature of e g 37 01C is shown Small fluctuations of the value are normal e Date in the format day month year e Time A programmed method for a photometric test can be called directly by input of the method number The valid range for a method number lies between 20 and 250 Scroll all existing methods by or If no method is programmed a plain text error message chapter 9 3 PLAINTEXT ERROR MESSAGES is shown Call the selected method by E Return to main menu by ESC Li A programmed method can be estab lished via menu METHOD NEW CHANGE COPY chapter 4 3 Method editor The transmission of a method collection is possible by PC with special software Further information Application sheets of reagent manufacturers 4 PROGRAM SELECTION 5010e 510 doc 16 01 08 4 2 Measurement with basic methods A photometric test can be executed by a method already permanently programmed but open in all setting parameters 14 different methods with different calculation procedures are available Each of these methods can serve as prototype for a method programmed by the operator BASIC METHODS PAGE 1 4 Available are e Absorbance measurement CONC W FACTOR PAGE p e Concentration measurement en
50. e first standard the program changes automatically to the method selection window There the given parameters of the respective method can be again controlled and or changed All further standards are queried during the following procedure The program for measuring the multi standards branches out automatically to the calculation procedures as follows CP1 gt CP5 CP2 gt CP6 CP3 gt CP7 CP4 gt CP8 CP9 gt CP10 CP11 gt CP12 CP14 gt CP14 i For measuring in another standard method than suggested start measuring multi standards in each other selected standard method before measuring the first standard by MODE MODE M STD Curve number and standards are queried accordingly MEASURE CURVE After inquiry of a curve number start of measuring of standard directly with a basic method which is based on standard This special mode ends automati cally after measuring or after selection of a factor method The standard of the selected basic method represents the first standard which can be measured All further standards are queried during the measuring execution in the special mode PRINT CURVE After inquiry of the curve number print out on the internal printer or via the serial interface Li For measuring in a multi standard method at least 2 bases with A and C must be defined 7 UTILITY PROGRAMS 5010e 510 doc 16 01 08 7 2 3 Printer ON OFF ROBERT RIELE GmbH amp Co KG Page 46
51. e from the joints of the pump tube Loosen the milled screw and remove the adaptor Another adaptor can easily be inserted Before check the cleanliness of the lense and the underside of the adaptor When inserting the adaptor electrical contacts in the device are closed Turn milled screw only by hand Working with original flow through system Lead the aspiration tube with attached bubble detector without sharp bend from the working area through the metal tube Fasten outlet tube cuvette to the metal nipple of the cuvette as well as to the joint of the pump tube A Before connecting the tubes remove pump tube at both ends from the metal clamps The pump tube must not be tensed during longer time out fig below Tension release The upper connection of the pump tube can be loosened easily from the metal clamps Plug the bubble detector to the socket in the working area It must sit as close as possible at the connection of the flow through cuvette For working without bubble detector switch off this function chapter 7 2 4 3 Bubble detector ON OFF A While working with the flow through system the tubes must not be sharply bended No residues should be inside Check from time to time that the tubing and the connections are leakproof After each replacement of tubing execute a Pump calibration chapter 7 2 4 2 A Before as well as after all measuring it is absolutely necessary to wash the tube system repeatedly with distilled w
52. e sales office immediately about transport damages Keep the original packaging for a possible return 2 2 PREPARATION FOR INSTALLATION Place the device on a stable level surface Do not obstruct the input air at the bottom and the waste air at the back plate to guarantee the ventilation of the device If the device was exposed to extraordinary fluctuation in temperature and or humidity it must acclimatize suffi ciently before operation A Before connecting the waste tube to the pump tube remove pump tube at both ends from the metal clamps The waste tube of the flow through system must be led through the tunnel to the backside of the device chapter 3 2 BACK and then into any drain tank 2 3 INSTALLATION Photometer 5070 operates at any line voltage between 90 Vac and 264 Vac at 50 60 Hz The device plug of the mains cable must be put into the socket at the back of the device and the mains plug into a grounded mains socket A While connecting or disconnecting an external device PC printer to Photometer 5070 both devices must be switched off Switch on Photometer 5070 by the mains switch at the back Greeting screen After switching on copyright website type of device and version designation are displayed and in the case of activated printer printed out ROBERT RIELE GmbH amp Co KG Page 9 2 INSTALLATION 5010e 510 doc 16 01 08 MAIN MENU METHODS MEASURE WITH PROGR MEASURE WITH BASIC METHODS NEW CHAN
53. easure standard 3 optional R 2 0 9994 ST KIN 0 327 Averaged standard FACTOR 244 3 T Resulting factor Ng nus 4 one Ser gt Insert measure sample 1 0 232 Ve ay Numerator ABS 1 ABS 2 Result R 2 0 9984 2 0 175 81 8 RS 0 3337 Insert measure sample ROBERT RIELE GmbH amp Co KG Page 35 5 CALCULATION PROCEDURES 5010e 510 doc 16 01 08 5 4 13 Calculation procedure 13 TRANSMISSION Calculation procedure ssssssssseeeeee CP 13 Characteristic ooooccccccocononncinnoncnonnonnconnoncnonnornnnnnancnnnno T in 96 Start method selection in the main menu PHOTOMETER 5010 5100 See chapter V5 10a 05 07 07 D 4 1 Measurement with programmed methods LAB RIELE GMBH CO KG 4 2 Measurement with basic methods DATE 07 10 07 TIME 11 24 32 In the case of activated printer the print out of the method data METHOD 13 TRANSM follows PROGRAM 13 FACTOR 1 0 The measuring window is shown WAVELENGTH 546nm TEMPERATURE 37C MEAS VOLUME 900ul WASH VOLUME 1000ul DELAY 2s UNIT Method procedure MEASURE 100 gt Insert measure zero solution NO ABS RESULT 1 0 329 46 9 gt Insert measure sample 2 1 004 9 9 Insert measure sample 3 2 020 1 0 gt Insert measure sample ROBERT RIELE GmbH 8 Co KG Page 36 5 CALCULATION PROCEDURES 5010e 510 doc 16 01 08 5 4 14 Calculation procedure 14 C F Delta Method at which a difference of sample
54. easuring Start method selection in the main menu PHOTOMETER 5010 5100 See chapter V5 10a 05 07 07 D 4 1 Measurement with programmed methods LAB RIELE GMBH CO KG 4 2 Measurement with basic methods DATE 07 13 06 TIME 10 03 16 In the case of activated printer the print out of the method data METHOD 30 CREATININ follows PROGRAM 10 STANDARD 2 00 The measuring window is shown WAVELENGTH 492nm TEMPERATURE 37C MEAS VOLUME 900ul WASH VOLUME 1000ul INCUBATION 45s REACTION 60s MAX UNITS 25 UNIT mg dl Method procedure MEASURE BLANK gt Insert measure zero solution Rb A 0 000 Without reagent blank insert measure optionally ST KIN 1 0 194 gt Insert measure standard 1 ST KIN 2 0 203 gt Insert measure standard 2 optional ST KIN 3 0 214 gt Insert measure standard 3 optional ST KIN 0 204 Averaged standard FACTOR 9 80 7 Resulting factor NO ABS RESULT 1 0 326 9 84 DELTA A 1 005 gt Insert measure sample 2 0 336 10 81 DELTA A 1 103 gt Insert measure sample 3 0 329 12 84 DELTA A 1 310 gt Insert measure sample ROBERT RIELE GmbH amp Co KG Page 33 5 CALCULATION PROCEDURES 5010e 510 doc 16 01 08 5 4 41 Calculation procedure 11 KIN F Rb Method at which a sample S is measured several times depending on the number of deltas in an equidistant time grid From the resulting absorbance values an alteration per minute AAs Minute i
55. edical devices stated in DIN EN 61010 testified by TUV Rheinland Group The Photometer 5070 fulfils the EMC immunity requirements for laboratory use equipment according to the EMC standard EN 61326 ROBERT RIELE GmbH amp Co KG Page 3 GENERAL NOTES 5010e 510 doc 16 01 08 Operator qualification Only appropriately trained operators are qualified to operate the device Environmental conditions The Photometer 5070 is approved for indoor use only For further environmental conditions see chapter 10 1 Patient ambience The Photometer 5010 may not be used in the patient ambience A Electrical Safety This device was examined and left the factory in perfect technical condition To preserve this and protect safe and faultless operation the operator must follow the orders and remarks of this operating manual Connect the device to grounded power outlets only All peripheral devices that are connected to the Photometer 5010 must comply with safety standard EN 60950 Before connecting read the documentation of the peripheral devices Opening covers or removing parts of the instrument except where this can be achieved manually without the use of any tool may expose voltage carrying components Connectors can be live too Never try to maintain or repair an open instrument which is carrying voltage Repairs at the device including replacement of the Lithium battery may be carried out only by authorized specialist staff Through improper re
56. eneous solution the input of a delay before a measuring is possible at all methods e All delay times can be cancelled by pressing the aspiration tube P for a long time ROBERT RIELE GmbH amp Co KG Page 18 5 CALCULATION PROCEDURES 5010e 510 doc 16 01 08 5 1 4 Fundamental to the methods with standard Each measuring of a standard calibrator can be executed as single double or triple determination Following is shown In the white reading window the MEASURE averaged absorbance of the standard 0 l 6 1 5 is shown ST A Below the white reading window the absorbance 1 2 and 3 of a standard are shown Glucose ooo ooo ermer NNO By OK the average of all values is taken over Values with 0 are ignored OK CURS DEL RESULT and excluded from the calculation The resulting factor is calculated from 36 99C 07 13 07 12 44 the average of the standard By CURS a value is selected A flashing white square marks the current value By DEL a value is deleted and excluded from the calculation By RESULT a measuring is triggered The determined resulting factor of a standard measurement is stored together with the corresponding method number After renewed selection of this method the last resulting factor is offered as OLD STD The principle of the multiple measurement can also be expanded to all measurements The corresponding entry can be set invoking a basic method The parameter is definable in pre
57. est check and adjustment of bubble detector 7 2 4 404 Pump ON OFF The current status of the pump is indicated in the first line by OFF or ON Change setting by SELECT Save setting permanently by OK Save setting temporarily up to next switch off of the device by EXIT 7 2 4 2 Pump calibration The pump calibration can be executed with or without bubble detector So set bubble detec tor ON or OFF Empty aspiration tube by WASH Put 1000 pl distilled water into a sample cup Dip aspiration tube into the sample cup up to the base The volume of 1000 yl is sucked in by Sipping lever P Behavior with bubble detector After stoppage of the pump values for air and water are indicated which correspond to the delivered volumes Save setting permanently by OK If the calibration is not possible although the pump tube is connected and 1000 ul were sucked in execute a media test see 7 2 4 4 Afterwards the pump calibration can be repeated 7 UTILITY PROGRAMS 5010e 510 doc 16 01 08 PUMP CALIBRATION AIR 159 WATER 1096 MS 50u1 EXIT 0K WASH B DET ON EXIT 0K SELECT VOLUME OPTIMIZED ON EXIT 0K SELECT ROBERT RIELE GmbH amp Co KG Page 48 Behavior without bubble detector With each operation of Sipping lever P the pump wheel rotates and delivers air The turn is shown beside the entry AIR on the screen As soon as the liquid in the tube between the metal inlet tube and the screw conne
58. fication A 1 Type of photometer Photometer 5010 A 2 Model 5010 A 3 Instruction for use Photometer 5010 user manual A 4 Manufacturer ROBERT RIELE GmbH amp Co KG Kurfuerstenstrasse 75 79 D 13467 Berlin Germany DECLARATION OF CONFORMITY The above mentioned absorption photometer is in conformity with the following metrological description Berlin March 2006 ROBERT RIELE GmbH amp Co KG Zu Links W Riele B Metrological description B 1 Measuring System B 1 1 Optical configuration cf Figure B 1 2 Source s of radiation halogen lamp B 1 3 Spectroscopic apparatus filter wheel B 1 4 Radiation detector s photodiode B 1 5 Cuvette s cell s 10 mm glass or plastic cuvette square shaped or 10 mm flow through cuvette B 1 6 Temperature regulation of the cuvette 25 C 30 C or 37 C B 1 7 Displayed units absorbance mass concentration enzyme activity B 1 8 Display device digital display absorbance 0 000 to 2 500 mass concentration 0 000 to 9999 enzyme activity 0 000 to 9999 B 2 Method of measurement B 2 1 Generation of spectral absorbance A A monochromatic measurement B 2 2 Zero compensation of spectral absorbance manual B 2 3 Control of the measured spectral absorbance with an absorption reference solution see manual B 2 4 Determination s of concentration Lambert Beer Equation ROBERT RIELE GmbH amp Co KG Page 70 10 TECHNICAL DATA 5010e 510 doc 16 01 08
59. ie Mka dea BRA ke ARE cated 55 7 2 8 MR I ofer 56 12 9 Temperature ON VOFF eee ente rte kn RAE EE EE n P n Eoo ena EU tn 57 7 2 10 Temperature adjustment eee ee Dee nein de rase e toe ioc e eed dense 57 7 2 11 Laboratory MAME t PERLE Er e RP ERE err nete Rete E nhan 58 7 2 12 User name ete ee e te ipae ee beu er edes Pe eco ee ie 58 IEERENSJa S 59 F214 Key signal ON OFF eire trece deals 59 7 2 15 Touchscreen adjustment aret ere e Er nei c eB e e en dto Ree dap dudes 59 2 16 AD ATC VIMO aee ree HER MEHR rr be nii nr Aii E tunes 60 TRAMO LANA kiye siek ete reyon Ye ye she ea ma Ye se a do 60 2 18 ADC counts Optio nice da cased des cocida ponn 61 1 2 19 Service tools eret iet e te yo kafe in prets ap e f ara ei aapna 61 8 MAINTENANCE iii ii a a at oo no a a on e a op m m ka A ako 62 8 1 CLEANING INSTRUCTION eterne cepi ad kad kinn 62 8 2 CALIBRATING MEASURING SYSTEM eee enn aoos se aoaoesseeoooosseoosoesoooossseoooonnonon 62 8 3 ADJUSTMENT OF BUBBLE DETECTOR ssssesseseeeeene enne e enne en nennen nere enne nnns 62 8 4 CALIBRATION OF PERISTALTIC PUMP emm emere enen nenne enne nnns 62 8 5 REPLACEMENT OF RIBBON tete eene e vest iae ut e S ak eI eee een pe an beeen sod 62 8 6 REPLACEMENT OF PAPER ROLL esee eene eene enne en nennen nenne enn nen nnns 63 8 7 REPLACEMENT OF ASPIRATION TUBE eene
60. ily to extend the lifetime Activate the lamp protection by ON Deactivate the lamp protection by OFF After 60 s Photometer 5010 is again ready for measuring Leave the function by EXIT Li A zero adjustment should be repeated by bringing in a zero solution and pressing ZERO Pressing LF in the main menu triggers a line feed in the case of activated printer Several lines can be advanced by continuous pressure on LF 4 PROGRAM SELECTION 5010e 510 doc 16 01 08 5 1 GENERAL NOTES The device offers operator guidance in the display by a combination of plaintext and short terms Messages and inputs regarding the method always have to be confirmed by OK By EXIT all methods can be broken off For a restart see chapter 4 PROGRAM SELECTION Measuring is generally triggered by sipping lever P or RESULT zero measuring by ZERO and sipping lever P chapter 3 5 2 Working with original flow through systemand 3 5 3 Working with standard cuvettes 5 1 1 Fundamental to the handling e Before measuring with standard cuvettes the lid of the cuvette compartment is to be closed e Deviations from normal operation caused by the device or by the operator are notified by ERROR They always have to be confirmed by E chapter 9 ERROR MESSAGE CORRECTION Example 1 The reading exceeds the programmed upper limit Example 2 Too little liquid when sucking in a measuring solution 5 1 2 Fundamental t
61. ivity levels VO V7 are calibrated in succession at simultaneous display of the dark value The function cannot be interrupted After com pletion the program returns to the utility program level Li Monthly executed the dark adjustment compensates possible deviations of the measuring accuracy due to environmental influences ROBERT RIELE GmbH amp Co KG Page 44 7 UTILITY PROGRAMS 5010e 510 doc 16 01 08 7 2 2 Multi standard functions Before curve data of a method with multi standard can be processed the method must have been established in the method editor chapter 6 METHOD EDITOR A curve without corresponding method cannot be processed The term curve no has the same meaning as method no If using a multi standard method later on pay attention that all extinction values of the samples lie within the range of the curve bases Values outside of the extinction range cannot be calculated In this case is shown and lt lt lt gt gt gt printed instead of the reading Fig 7 2 2 1 UTILITIES MEASURE CURVE PRINT CURVE EDIT CURVE SHOW CURVE 37 05C Oo WS O 7 Print out of curve CURUES 20 07 03 06 A k i LA SUB MENUE ESA EXIT det 3 9 NO ABS CONC i 0 202 60 2 0 60 30 3 0 920 15 4 1 160 73 5 501 3 28 ROBERT RIELE GmbH 8 Co KG Page 45 Main window of multi standard functions MEASURE CURVE After query of a curve number and th
62. lank press ENTER Calculation procedure Characteristic Method cccccceceeeeseseeeeeeeeaneees Calculation formula C F AAsuinit AARB Minit Resulting factor F Cst AAST Minit AARB Minit Reagent blank rirrrrrrerereererrrororres entering or measuring Number of deltas entering 3 to 28 Time per delta suuussl entering 4 s to 255 s Start method selection in the main menu PHOTOMETER 5010 5100 See chapter V5 10a 05 07 07 D 4 1 Measurement with programmed methods LAB RIELE GMBH CO KG 4 2 Measurement with basic methods DATE 07 09 07 TIME 11 10 03 In the case of activated printer the print out of the method data METHOD 32 UREA follows PROGRAM 12 STANDARD 80 0 The measuring window is shown WAVELENGTH 340nm TEMPERATURE 37C MEAS VOLUME 900ul The absorbance value will be constantly refreshed on the display WASH VOLUME 1000ul during the delay time DELAY 35 At the beginning and at the end of the delay time the absorbance DELTAS 5 values ABS 1 and ABS 2 are measured respectively TIME DELTA 5s MIN R 2 0 998 UNIT mg dl Method procedure inc MEASURE BLANK m gt Insert measure zero solution Rb A 0 000 Without reagent blank insert measure optionally ST KIN 1 0 327 gt Insert measure standard 1 R 2 0 9996 ST KIN 2 0 330 gt Insert measure standard 2 optional R 2 0 9989 A ST KIN 3 0 324 Insert m
63. m 2 MONTHLY MEMORY S2 DATE 07 10 07 PHOTOMETER 5010 5100 V5 10a 05 07 07 D LAB RIELE GMBH CO KG METHOD 25 GLUCOSE UNIT mmol l SERUM NO 5 ID LT SYS abnormal LOT G312 COMPANY LABOR TECH DATE MAY 06 REQUIRED 14 4 MIN VALUE 12 1 MAX VALUE 16 7 QC VALUES n 20 MEAN m 13 860 STD DEVIATION s 1 012 COEFF OF VAR CV 7 298 02 15 06 13 54 1s 1 02 14 06 14 07 41s 1 02 13 06 14 69 41s 1 02 12 06 13 50 1s 3 02 11 06 14 68 41s 3 02 10 06 15 33 42s 1 02 09 06 15 99 3s 1 02 08 06 15 38 42s 2 02 07 06 14 61 41s 1 02 06 06 13 70 1s 1 02 05 06 12 74 2s 1 02 04 06 12 13 2s 1 02 03 06 12 65 2s 2 02 02 06 13 11 1s 1 02 01 06 13 88 1s 3 01 31 06 13 51 1s 3 01 30 06 13 24 1s 3 01 29 06 12 50 2s 1 01 28 06 12 74 2s 2 01 27 06 13 30 1s 1 ROBERT RIELE GmbH amp Co KG Page 54 Output dialog If at least 20 readings are stored in the monthly memory these are indicated in the Levey Jennings plot In this representation the deviations can be controlled visually and thus tendencies or systematic errors be better recognized The keys for the printout are located next to the curve diagram PRN N Start the normal printout of the data of the current monthly memory The single data of the readings are not printed thereby PRN E Start the extended printout of the data of the current monthly memory As shown in the example left also the single data of the readings are printed EXIT Re
64. me X incubation time in seconds iia At Fixed Time reaction time in seconds Tape eRe At Kinetic time per delta in seconds ROBERT RIELE GmbH amp Co KG Page 21 5 CALCULATION PROCEDURES 5010e 510 doc 16 01 08 5 3 SURVEY OF THE METHODS The calculation procedures on which all methods are traceable from the list of methods are mentioned in the following table Criterion is the characteristic of the calculation procedure see below For detailed description of the respectively accompanying procedure of method see chapter 5 4 DESCRIPTION OF METHOD PROCEDURES CP No Characteristic Method Calculation formula CP 1 C F Endpoint with Factor C FxAs CP 2 C F Rb Endpoint with Factor C F As Ang CP 3 C F Sb Endpoint with Factor C F As Ase CP 4 C F SbRb Endpoint with Factor C F As Asg Ars Arsel CP 5 C S Endpoint with Standard C FxAs CP 6 C S Rb Endpoint with Standard C F As Ang CP 7 C S Sb Endpoint with Standard C F As Ase CP8 C S SbRb Endpoint with Standard C F x As Asel Ars Arsel CP 9 FTK F Rb Fixed Time Kinetic with Factor C F Aso As Aro Ars CP 10 FTK S Rb Fixed Time Kinetic with Standard C F Aso As Aro Ane l CP 11 KIN F Rb Kinetic with Factor C F AAs minit AARB Minit CP 12 KIN S Rb Kinetic with Standard C F AAs minit AARB Minit CP 13 TRANSM Transmission in 96 CP 14 C F
65. me with E1 E2 In the second course will be so many samples measured as in the first course Before starting a new E1 E2 course a new zero measurement has to be done The reagent time 3 is usable only if the reagent time 2 is set In that case the reagent mode will be entered i e the photometer also takes control of the timing for dispensing the reagent before measuring the samples The quantity of samples will be determined by the reagent time 2 and the time delta The reagent time 2 should be chosen less than or equal to the reagent time 3 and greater than or equal to the time delta Figure 5 1 shows the time sequence of a time controlled measuring process with N samples a delay time T3 and without reagent time A Ei Y O T3 01 Ti TA 1 1 1 LI I L 1 l T2 Blank Press Insert Insert Insert measure Result En E12 E13 t t Time T1 Time Delta a automatic process T2 Measure time T3 Delay optional A C manual process A Start of time controlled measuring El Start of first course El E2 Start of second course E2 Fig 5 4 14 1 time controlled measuring ROBERT RIELE GmbH 8 Co KG Page 37 5 CALCULATION PROCEDURES 5010e 510 doc 16 01 08 Calculation procedure Characteristic rrrerrrrrorroreoooon Calculation formula PAC OM past E E EE ka Soins Sample blank suussse Time Delta T1
66. mory contents remain after switching the device off If the memory is filled with 2970 results no further result can be added The memory must be emptied first by deletion of results The logging menu offers following options UTILITIES SUB MENUE LOGGING ON OFF Activation of data log LOGGING ON OFF ging If this mode is activated the sample numerator gets a new function as ID no Then PRINT DATA OF ID NO before each measuring the numerator must be set to the required number between 1 and 99 by MODE and NUM After each measuring DELETE DATA OF ID NO the reading is asked to be stored REPORT OF LOG MEMORY EXIT 37 00C 07 13 07 14 52 PRINT DATA OF ID NO After query of the ID no the corresponding readings are printed out Afterwards the printed data are asked to Print out of readings of an ID no be deleted DATA FROM ID NO LES IIS S e LUN DELETE DATA OF ID NO After query of DATE 07 09 07 the ID th di di LAB RIELE GMBH CO KG e no e corresponding readings are deleted ID NO 35 DATE 02 28 06 REPORT OF LOG MEMORY Print out of a report of the complete logging memory with ID no and corresponding number of readings METHOD 24 GLUCOSE 13 03 mmol l DATE 03 01 06 e g METHOD 25 CK MB s 195 U l 5 3 stands for 3 stored readings for ID no 5 DATE 03 02 06 METHOD 24 GLUCOSE 12 17 mmol l EXIT Return to the utilities Report of logging memory LOGGING REPORT PHOTOMETER 5
67. n time To ARE kwe men At Fixed Time absorbance of reagent blank after reaction time T ARBB or veeerooooooooooorene Absorbance of blank of reagent blank Ant Absorbance of sample Astana kwi At Fixed Time absorbance of sample after incubation time To a At Fixed Time absorbance of sample after reaction time T NSB sevo eene ene Absorbance of sample blank AST odes Absorbance of standard PAST 0 font dert papay ete At Fixed Time absorbance of standard after incubation time To AST sta At Fixed Time absorbance of standard after reaction time T ASTB vic eite Absorbance of standard blank Cuidados Concentration e Concentration of standard CM as uinacmue Quality control Coefficient of variation dA min At Kinetic AA min AARB Minit eee At Kinetic change of reagent blank per minute measured in AA min AAS Minit At Kinetic change of sample per minute measured in AA min Fuit Factor ET Fixed Time Kinetic KIN Cisco ee Kinetic Musa desd Quality control number of values MM Nanometer dimension of wavelength Miranda Quality control mean of values Posada av Result Sample RO zi ata Reagent blank RDD 5 nes Blank of reagent blank Recta At Kinetic square of correlation coefficient shows the linearity of a test SON Standard STD tcc Standard blank OD ers Sample blank Shas retorts Aesth Quality control standard deviation TRANSM T Transmission in orcas idad At Fixed Ti
68. nection of aspiration tube and outlet tube cuvette Switch on the pump by the function Pump ON OFF chapter 7 2 4 1 Switch on or off bubble detector by the function Bubble detector ON OFF chapter 7 2 4 3 After the installation execute a Pump calibration chapter 7 2 4 2 ROBERT RIELE GmbH 8 Co KG Page 14 3 OPERATING ELEMENTS 5010e 510 doc 16 01 08 4 PROGRAM SELECTION After switch on the touchscreen shows the main menu From this screen the basic methods unalterably programmed in the system or operator specific programmed methods can be reached Also the adjusting programs are started from this mask With the method editor own methods can be established and changed The utility programs cover the configuration adjustments and check routines The lamp protection function can directly be reached by LAMP line feed of printer by LF After completion of a method or execution of a utility program the program always returns to the main menu MAIN MENU MEASURE WITH PROGR METHODS MEASURE WITH BASIC METHODS METHODS NEW CHANGE COPY Wyte bt yr ae S LAMP LF 31 036 OZE MIS 2057 it ak 3s amp 4 1 Measurement with programmed methods METHODS NO 30 METHOD Glucose UNIT mg dl 1 2 3 4 5 6 7 8 EFS CITO 0 E ROBERT RIELE GmbH amp Co KG Page 15 Main menu Down in the status line from left to right following is shown e Current temperature of the cuvette adaptor in C In the case of switched off tempering th
69. nennen nennen nnne nnne n rnnt en 63 8 8 REPLACEMENT OF LINE FU SES 5 ee ib dee t een era de IR ede peo deinen a dua de t aie 63 9 ERROR MESSAGE CORRECTION 00 cccccscssseeeeeeeeeeeeeeeeeeeeneeeeeeeeeeeeeeees 64 9 1 GENERAL NOTE oe ei e HRS kant kd n SEN E UNE RA Biete RR Ak a eR EN UR ERN NUR clr L e Me E RP RES 64 9 2 ACOUSTIC ERROR MESSAGES eise a cada 64 9 3 PLAINTEXT ERROR MESSAGES ssseseeeeeeeeene nnne asse sone ennt ener en nenne nennen nns 64 94 CODED ERROR MESSAGES inr em pi maten E nir Ree ERES 64 10 gt TECHNICAL DATA iiis Saxonia tix eaae w Ln FR dei ek Cx Li ix Lid 67 10 1 ENVIRONMENTAL CONDITIONS seen nenne een neneem ener en nenne nennen nene rennen en 67 10 2 MINIMAL OPERATION QUALITY sssesseseeeeenenneeneneen nennen nennen rnnt n rnnt en nnne nnns 67 10 3 TYPESPENTE teer n N knnen s e ket REEL ERR Rein der EE Ree AT ASE 67 10 4 SHORT SPECIFICATIONS imitar eei eee eie eet Erde ete ee nca poteet be Tee Sen dp ge 68 10 5 CERTIFICATE Sirri irc iei oe tercie ta Beer te oett d p e Rast fe aso de BOE ka touv EAEE loa saa 69 11 ACCESSORIES AND SPAREPAR Tunisia 72 12 METHOD LIS T pepe rate sesttezetecdtazutocd tapum ant tatutond canon Pet abea been ooo bw pea b et mE tb VENUE 73 12515 BASIC METHODS 2 s peter pan bos As 73 12 2 LIST OF USER SPECIFIC METHODS sese eene nennen enne en nennen nennen 74 ROBERT RIELE GmbH amp Co KG
70. o KG Page 30 5 CALCULATION PROCEDURES 5010e 510 doc 16 01 08 5 4 8 Calculation procedure 8 C S SbRb Method at which the difference of reagent blank Arg and blank of reagent blank Arse regarding the amount is subtracted of the difference of sample value As and sample blank Asg regarding the amount and this difference is multiplied with a factor F which is determined by measuring of a standard solution with known concentration Csr and under consideration of standard blank Astg and the difference of reagent blank Arg and blank of reagent blank Arse The sample blank Asa is measured before every test The reagent blank Arg is entered or measured once Calculation procedure 00 0 eeseeeeeseeeeeeenneeeeeeneeeeenneeeeneas CP 8 Characteristics C S SbRb A ruins tou devise dese syon End Point with Standard Calculation formula C F As Ass Ars Angel Resulting factor Fz Cst Ast Asre Akg AReBe Reagent blank rrrrrrrrrrereeoerererorres entering or measuring Start method selection in the main menu PHOTOMETER 5010 5100 See chapter V5 10a 05 07 07 D UNIT mg dl MEASURE BLANK 4 1 Measurement with programmed methods LAB RIELE GMBH CO KG 4 2 Measurement with basic methods DATE 07 13 07 ZEIT 09 33 26 In the case of activated printer the print out of the method data METHOD 28 Ca follows PROGRAM 8 STANDARD 8 02 The measuring window is shown WAVELENGT
71. o the tempering e Tempering switched on or off is parameter of a method e After switching on the tempering it lasts up to 10 minutes until a constant temperature of 25 C 30 C or 37 C is reached e The current temperature of the flow through cuvette or of the cuvette adaptor is shown at the lower edge of the touchscreen For meaning of the display see chapter 4 PROGRAM SELECTION MAIN MENU A temperature instable or out of tolerance during measuring is marked by an asterisk at the utmost right position in the corresponding print line To avoid deviations due to temperature influence a delay between triggering and actual measuring can be programmed in each method e For a quick mode of operation all temperature sensitive samples reagents and washing solutions should be externally temperated by Incubator T12 T16 REF 500 002 500 001 or a water bath 5 1 3 Fundamental to the inputs e The input format of the factor and or the standard with sign determines the output format of the result concerning the number of decimal places Example With factor 36 8 the calculated concentration will be shown with one decimal place e Each factor or standard can be minus signed so that the result is calculated with correct sign Example The test GOT is programmed with the factor 1746 because the measuring principle implies a decreasing absorbance e The reagent blank can be measured entered or put on zero e For a homog
72. occccccncococccccocincnanononccnncananannonnos End Point with Standard Calculation formula esses C F As Ase Resulting factor neen F Cs Asr Astel Start method selection in the main menu PHOTOMETER 5010 5100 See chapter V5 10a 05 07 07 D 4 1 Measurement with programmed methods LAB RIELE GMBH CO KG 4 2 Measurement with basic methods DATE 07 13 07 TIME 08 55 25 In the case of activated printer the print out of the method data METHOD 27 UREA COL follows PROGRAM 7 STANDARD 50 0 The measuring window is shown WAVELENGTH 546nm TEMPERATURE 37C MEAS VOLUME 900ul WASH VOLUME 1000ul DELAY 3s MAX UNITS 220 UNIT mg dl Method procedure MEASURE BLANK gt Insert measure zero solution gt Insert measure standard blank ST A 1 0 614 gt Insert measure standard 1 ST A 2 0 629 2 Insert measure standard 2 optional ST A 3 0 620 2 Insert measure standard 3 optional ST A 0 621 Averaged standard STb A 0 106 standard blank DELTA ST 0 515 4 Averaged standard minus standard blank FACTOR 97 1 Resulting factor NO ABS RESULT 1 2 292 197 6 gt Insert measure sample blank Sb A 0 257 gt Insert measure sample 5 2 340 198 0 gt Insert measure sample blank Sb A 0 300 gt Insert measure sample 3 2 223 197 2 gt Insert measure sample blank 0 193 gt Insert measure sample Sb A ROBERT RIELE GmbH 8 C
73. ometer 5070 against defects in material and workmanship For further information contact the local distributor At the end of the life or utilization time the device and the accessories can be given back to the manufacturer with costs for an environmental waste disposal The previous professional decontamination has to be proved with a certificate Address of the manufacturer aud ROBERT RIELE GmbH amp Co KG Kurfuerstenstrasse 75 79 D 13467 Berlin Germany Phone 49 0 30 404 40 87 Fax 49 0 30 404 05 29 E mail info riele de http www riele de ROBERT RIELE GmbH amp Co KG maintains a quality management system according to DIN EN ISO 9001 2000 and DIN EN ISO 13485 2003 certified by TUV Rheinland Group CERTI ROBERT RIELE GmbH amp Co KG Page 5 GENERAL NOTES 5010e 510 doc 16 01 08 CONTENTS 1 INTRODUCTION TO PHOTOMETER 25010 eeeeeeeeee 8 2 INSTALLATION iii 9 2 1 DELIVERY 2 55 detti testo a Doce Meta edt ode ie oi a po defi oka pe Aa epa ak bade ab oo dan 9 2 2 PREPARATION FOR INSTALLATION 20 lL vil ettt tk kaa eat aa aaa ae nennen nennen rnnt innen terrere nnns 9 2 9 INSTALLATION en cee alin ve seke e oue se oar ee Y Aa yo veye aes 9 2 4 LOADING PRINTER PAPER oem nipona osito like dove dow joer 10 3 OPERATING ELEMENT Sound 11 3 1 meh 11 32 BACK EE 11 3 3 TOUCHSCREEN 5 inerte as 12 234 WORKING AREA 5 iti EE inicia ias 12 3 5 CUVETTES AND CUVETTE ADAPTOR
74. ononn cocoa nn nc nano nennen nennen enne 32 5 4 10 Calculation procedure 10 FTK S RD ooococoncccnococcnononcnonononcnnnono cocoa nn ncnnnn nennen nennen enne 33 5 4 11 Calculation procedure 11 KIN F RD ooooonoccconococcnononcncnononcnn nono nonnnnn cnc nano nn nr nono emnes 34 5 4 12 Calculation procedure 12 KIN S RD oooconocccnnococcconoccnccononcnnnononcnnnonccnonnn meme nnne nnns 35 5 4 13 Calculation procedure 13 TRANSMISSION seen eene nennen 36 5 4 14 Calculation procedure 14 C F Delta oooooonccnnococcnonocccccononcnnnononcnnnonnncnnnn cnn r non n em emen 37 5 4 15 Calculation Procedure 15 C F 3 WL ikisinin tonii ieina nn nr non nennen enne nnns 39 6 METHOD EDITOR coseno rl 40 ROBERT RIELE GmbH 8 Co KG Page 6 CONTENTS 5010e 510 doc 16 01 08 ZI UTILITY PROGRAMS kri ok n kk na ak ken rior tan rer rre ctn re a 43 7 1 SELECTION OF UTILITY PROGRAMS viad niee a non n nn naar n nn en nere enne nennen nnne 43 7 2 DESCRIPTION OF UTILITY PROGRAMS issssseeene ene nennen nnnm nnn en nnne nnne nnns 44 7 2 1 Dark adjustmiebit hte to id et oreet ik leelo de ake kode p p k kte kb ka don ap led 44 2 2 Multl standard TUnGtions oct titanes 45 1 2 3 Printer ON VOFF ui EE 46 52 1 PUMP Mes o ea ect aero ld 47 A425 Serial interface e Ee me HE eee cde d 50 E26 Quality COMO Rer oben ei 51 AP eT PMIRTOUTSOTINGS sce Maat eret eo retine chan oak kaa idem vivant loose cide
75. pairs the warranty extinguishes and the operator can be heavily jeopard ized If suspected the device can no longer be operated safely turn it off and take steps to ensure that no one will subsequently attempt to use it Electromagnetic waves Devices that emit electromagnetic waves may affect measured data or cause the Photometer 5070 to malfunc tion Do not operate the following devices in the same room where the Photometer 5070 is installed mobile phone transceiver cordless phone and other electrical devices that generate electromagnetic waves A Reagents Regarding reagents follow the safety as well as the operating instructions of the manufacturers Pay attention to the currently valid German Gefahrstoffverordnung GefStoffV A A Biological safety Liquid waste is potentially biologically hazardous Always wear gloves if handling those materials Do not touch parts of the device other than those specified Consult the laboratory protocol for handling biohazardous materials Pay attention to the currently valid German Biostoffverordnung BioStoffV A Spillings and cleaning If a sample is spilled on the device wipe up immediately and apply disinfectant A Waste Handle liquid waste properly according to legislation on water pollution and on the treatment of drainage and waste matter ROBERT RIELE GmbH amp Co KG Page 4 GENERAL NOTES 5010e 510 doc 16 01 08 ROBERT RIELE GmbH amp Co KG warrants Phot
76. pro grammed methods chapter 6 METHOD EDITOR 5 1 5 Fundamental to the methods with multi standards Linear calibration is used in the case of two calibrators The absorbance forms a linear diagram with the concentrations chapter 7 2 2 Multi standard functions Nonlinear calibration is used for samples with a nonlinear but reproducible connection between the absorbance and the concentrations At least three maximum 20 calibrators are required for nonlinear calibration chapter 7 2 2 Multi standard functions 5 1 6 Fundamental to bichromatic measurements The calculation procedures based on endpoint measurement CP 1 to CP 8 CP 13 and CP 14 can be executed bichromatic The zero measurement will be done with a wavelength defined as bichromatic The bichromatic wavelength might be not included in the standard set of filters The bichromatic wavelength can be set after calling a method chapter 6 METHOD EDITOR Fig 6 5 ROBERT RIELE GmbH amp Co KG Page 19 5 CALCULATION PROCEDURES 5010e 510 doc 16 01 08 5 1 7 Fundamental to the Kinetic In a kinetic method the sample absorbance is measured several times in pre established time intervals The user can define a delay time and a quantity and duration of time intervals after the delay time Deltas or At At the beginning and at the end of the delay time the absorbance values ABS 1 and ABS 2 are measured respectively The difference ABS 1 ABS 2 allows the
77. re sample 3 1 033 4 23 gt Insert measure sample blank Sb A 0 702 gt Insert measure sample ROBERT RIELE GmbH amp Co KG Page 26 5 CALCULATION PROCEDURES 5010e 510 doc 16 01 08 5 4 4 Calculation procedure 4 C F SbRb Method at which the difference of reagent blank Arg and blank of reagent blank Arse regarding the amount is subtracted of the difference of sample value As and sample blank Asg regarding the amount and this difference is multiplied with a given factor F The sample blank Asg is measured before every test The reagent blank Arg is entered or measured once Calculation procedure sss CP 4 Char cteristio ne ek ape feat 0I C F SbRb A E End Point with Factor Calculation formula C F As Asgl Ars Angel zoo EEE E E E cacon pe sesa pase given entering Reagent blank cceeeeeeeesereeeeeeees entering or measuring Start method selection in the main menu PHOTOMETER 5010 5100 See chapter V5 10a 05 07 07 D 4 1 Measurement with programmed methods LAB RIELE GMBH CO KG 4 2 Measurement with basic methods DATE 07 09 07 TIME 10 11 15 In the case of activated printer the print out of the method data METHOD 24 Fe follows PROGRAM 4 FACTOR 1330 The measuring window is shown WAVELENGTH 578nm TEMPERATURE 37C MEAS VOLUME 900ul WASH VOLUME 1000ul DELAY 5s MIN UNITS 37 MAX UNITS 158 UNIT ug dl Method procedure MEASU
78. rt measure sample gt Insert measure sample gt Insert measure sample ROBERT RIELE GmbH amp Co KG Page 32 5 CALCULATION PROCEDURES 5010e 510 doc 16 01 08 5 4 10 Calculation procedure 10 FTK S Rb Method at which a reagent blank is measured after an incubation time gt Arso and after a reaction time gt Arg and also a sample after an incubation time As o and after a reaction time gt As 1 The difference from the change of the sample and the change of the reagent blank becomes multiplied with a factor F which is determined by means of the change of standard solution Asro Asr 1 and the change of reagent blank Ars o Ars 1 during the reaction time and given concentration of standard The reagent blank Arg is entered or measured once During the procedure the dialog asks for the use of a reagent blank The default value is OFF To continue with out reagent blank press ENTER After each measurement the next sample can be measured with NEXT With RESULT it is possible to measure the same sample again Calculation procedure ccccccceceeceeeeeeeeeeceeeeeeeeeeeeees CP 10 Characteristic sssssssse eene FTK S Rb Method needs Fixed Time with Standard Calculation formula C F x Aso As 1 ArB o Ang Resulting factor Fz Cst Ast o Ast 1 ARrB o ArB 1 Reagent blank rrrrrrrrerereeoerrrrrorren entering or m
79. s determined by a regression calculation The reagent blank AARB minute is measured in the same way as the sample or entered directly in U I and subtracted from the sample value This difference is multiplied by a given factor F During the procedure the dialog asks for the use of a reagent blank The default value is OFF To continue with out reagent blank press ENTER Calculation procedure Characteristic Method nere rindi Calculation formula FAC OR oeste tte a ettet Reagent blank Number of deltas Time per delta C F AAs minit AARB Minit given entering entering or measuring entering 3 to 28 entering 4 s to 255 s PHOTOMETER 5010 V5 10a 05 07 07 D 5100 LAB RIELE GMBH CO KG DATE 07 10 07 TIME 10 50 19 METHOD 31 GOT PROGRAM 11 FACTOR 1746 0 WAVELENGTH 340nm TEMPERATURE 37C MEAS VOLUME 900ul WASH VOLUME 1000ul DELAY 60s DELTAS 5 TIME DELTA 18s MAX UNITS 280 MIN R 2 0 998 UNIT U L MEASURE BLANK ELTA REG 0 0908 Rb A 0 000 NO ABS RESULT 1 0 123 R 2 0 9996 2 0 154 189 9 R 2 0 9993 3 0 110 158 5 R 2 0 9986 ABS 1 0 812 T ABS 2 0 702 1 0 0867 2 0 0899 3 0 0871 4 0 0874 5 0 1087 D Start method selection in the main menu See chapter 4 1 Measurement with programmed methods 4 2 Measurement with basic methods In the case of activated printer the print out of the method data follows The measuring windo
80. te character e gt blank e ESC finish input without storage e ENT finish input with storage 7 UTILITY PROGRAMS 5010e 510 doc 16 01 08 7 2 43 Error list 7 2 14 Key signal ON OFF 7 2 15 Touchscreen adjustment ROBERT RIELE GmbH amp Co KG Page 59 The last 10 serious errors are shown or printed The oldest error is shown first The last error is always marked with no 1 By NEXT earlier error messages are shown By PRINT the complete error list is printed or output to the serial interface For troubleshooting the coded error list can be consulted chapter 9 4 CODED ERROR MESSAGES The current status of the key signal is indi cated in the first line by OFF or ON Change setting by SELECT Save setting permanently by OK Save setting temporarily up to next switch off of the device by EXIT The deeper signal tone for error messages remains active in any case By this function the panel can be adjusted After call of the function a white cross is shown in the left lower corner of the screen Touch the intersection point in the cross with a non scratching plastic tip touchscreen pen pipette tip as exactly as possible In the first line the coordinates are shown as X and Y value The input will be accepted and the coordinate display will be reseted after a time out of 10s Then the cross is shown in the right upper corner Touch the intersection point After a timeo
81. th A and C must be defined 6 METHOD EDITOR 5010e 510 doc 16 01 08 UTILITY PROGRAMS SELECTION OF UTILITY PROGRAMS MAIN MENU Main menu Utility programs are necessary for the adjust MEASURE WITH PROGR METHODS ment and maintenance of the photometer MEASURE WITH BASIC METHODS MEASURE NEW CHANGE COPY UTILITIES LAMP LF 3 7 5 Wise ONES 6 0 7 al al 8 48 8 UTILITIES PAGE 1 5 Page 1 of utility programs Scrolling through all utility programs is possi DARK ADJUSTMENT PAGE ble by PAGE The current page is shown at the right upper screen corner By EXIT the MULTI STANDARD program returns to the main menu PRINTER A utility program is selected by pressing the relating key PUMP MENU EXIT SA One Oy is 7 13 05 Description in chapter Dark adjustment 7 2 1 Multi standard functions Printer ON OFF 7230 7 2 3 Serial interface Quality control 7 2 5 7 2 6 Printout settings Data logging 7 2 8 Temperature ON OFF Temperature adjustment 7 2 10 Laboratory name Error list Date Time 7 2 16 ROBERT RIELE GmbH amp Co KG Page 43 7 UTILITY PROGRAMS 5010e 510 doc 16 01 08 7 2 1 Dark adjustment The dark adjustment should be done not before the warm up time of 15 minutes has passed better after one hour operation Li To avoid strange light the lid has to be closed during the dark adjustment Start the dark adjustment by START Automatically all eight sensit
82. trol In Photometer 5070 up to 50 methods can be supervised with a quality control The device can manage up to 6 control serums Each QC supervised method can be connected with 2 control serums The QC data of a series of measurements are stored in a daily memory Each reading is stored with method number date and user identification From the daily memory the individual QC data can become deleted or saved in the monthly memory of the corresponding method The monthly memory of a QC method can record up to 31 readings With the 32nd the oldest reading is deleted in the memory For the calculation of the quality values of a method at least 20 readings in the monthly memory must be present The average of all readings the standard deviation and the coefficient of variation are calculated Contents of the daily and monthly memory can be indicated and printed out Except the basic methods all methods can be connected with a quality control The method typical data of a control serum are entered via the method editor see chapter 6 METHOD EDITOR UTILITIES SUB MENUE The QC menu offers following functions EDIT CONTROL SERUM Up to 6 control EDIT CONTROL SERUM serums can be defined Without a defined serum the QC cannot be started DAILY MEMORY DAILY MEMORY View print and process MONTHLY MEMORY ing of the daily memory for serum 1 2 MONTHLY MEMORY View print and proc QC EDP EXIT essing of the monthly memory for serum 1 2 37 00C 07 13
83. turn to QC menu 7 UTILITY PROGRAMS 5010e 510 doc 16 01 08 7 2 6 4 QC EDP Not implemented 7 2 7 Printout settings By START the program version and the complete status of the saved settings are printed out PRINTOUT SETTINGS EXIT START to ACTUAL SETS eee DATE 07 10 07 TIME 12 59 51 PHOTOMETER 5010 7001 V5 10a 05 07 07 D PCB LAYOUT C ADC COUNTS DARK ADJ E 0 18240 1 18244 2 18251 3 18261 4 18279 5 18312 6 18380 7 18494 ADC COUNTS DARK ADJ D 0 18235 1 18236 2 18236 3 18237 4 18240 5 18246 6 18255 7 18271 TEMPERATURE E 25C 8322 3000 30C 9552 3000 37C 11273 3000 TEMPERATURE D 25C 8299 3000 30C 9485 3000 37C 11165 3000 FILTER 0 DDD 2 405 4 546 6 623 8 999 PUMP MS AIR 201 MS 50ul 66 B DET 5 BATTERY OK VDC LAMP 12 25V ADC CORRECTION 150 BOOST nm 390 EDP OFF STX ETX BCC LANUAGE 1 ENGLISH 2 GERMAN KEY SIGNAL ON PROGR METHODS 0 340 492 578 999 999 ON UWP ROBERT RIELE GmbH amp Co KG Page 55 7 UTILITY PROGRAMS 5010e 510 doc 16 01 08 7 2 8 Data logging Up to 2970 results can be managed in the memory of Photometer 5010 They are stored together with a two digit identification number which can be assigned e g to a patient or to a sample The range of the identification number starts with 1 and ends with 99 Up to 2970 results can be assigned to one identification number or aver age 30 results for maximum 99 identification number stored Me
84. ulation methods 1 915 up to 231 user specific methods chapter 12 2 LIST OF USER SPECIFIC METHODS as copy master to be filled out by the user BASIC METHODS 16 19 20 250 12 1 syn ey sun UN BEN uopesy LL oneuny uoneqnou ejeg paepuejs 10398 4 uu Y onset euo LLL awen poule m 12 METHOD LIST Page 73 ROBERT RIELE GmbH amp Co KG 5010e 510 doc 16 01 08 LIST OF USER SPECIFIC METHODS 12 2 spun xen suun un gut Buueduie uonoees LI oneury uogeqnou Aejaq plepuejs 101984 12 METHOD LIST Page 74 ROBERT RIELE GmbH 8 Co KG
85. ut of 10s the memory inquiry follows Save the adjustment by OK Reject the adjustment by EXIT 7 UTILITY PROGRAMS 5010e 510 doc 16 01 08 Hint If the device is maladjusted this function can directly be called during the switching on routine Keep the touchscreen pressed during switching on After some seconds a deep signal tone sounds and the text message TOUCH PANEL ADJUSTMENT will be shown at the first line of the greeting screen Release the touchscreen within one second Execute the adjustment of the touchscreen as described above You must choose your Photometer type 4040 or 5010 after the touchpanel adjustment is completed 7 2 16 Date Time 7 2 17 Language ROBERT RIELE GmbH amp Co KG Page 60 Select Photometer This dialog will be only displayed when the touchscreen adjustment has been entered during switching on the Photometer The Photometer type in use must be selected 4040 or 5010 The current status of the date time display is indicated in the first line by OFF or ON Change setting by SELECT With activation of the clock date and time can be changed by OK Each entry of day month year minute and second must be confirmed by E If a value is to be changed then all values are to be entered again The current status of the language is indicated in the first line The setting can be changed by SELECT Following options are possible e LANGUAGE ENGLISH e LAN
86. w is shown This example shows a negative factor producing a positive result at decreasing absorbance The absorbance value will be constantly refreshed on the display during the delay time At the beginning and at the end of the delay time the absorbance values ABS 1 and ABS 2 are measured respectively Method procedure gt Insert measure zero solution Without reagent blank insert measure optionally gt Insert measure sample 189 7 Numerator ABS 1 ABS 2 Result Insert measure sample gt Insert measure sample Detail print out of deltas ABS 1 and ABS 2 ROBERT RIELE GmbH amp Co KG Page 34 5 CALCULATION PROCEDURES 5010e 510 doc 16 01 08 5 4 12 Calculation procedure 12 KIN S Rb Method at which a sample S is measured several times depending on the number of deltas in an equidistant time grid From the resulting absorbance values an alteration per minute AAs Minute is determined by a regression calculation The reagent blank AARB minute is measured in the same way as the sample or entered directly in U I and subtracted from the sample value This difference is multiplied by a factor F which is determined by meas uring of a standard solution AAst Minute With known concentration Cst and under consideration of the reagent blank AARB minute During the procedure the dialog asks for the use of a reagent blank The default value is OFF To continue with out reagent b

Bedienungsanleitung Photometer 5010

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