SOP: To Create, your run protocol, optimize and run an assay. For
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3. art Wizard The mice vl heip pou to start temasya the pequred opk nica If meoestary hka wang he mest A s possble to perfor yar ous oir eatis het sharony the aay Proud to prr fermegtmentionls don Fd bweirsirictems r thn word F yo Tar van o mwt ve sca Pasea 1034 the pe WEN sans PE PETAN 80 14 When complete your protocol will be optimized and ready to run 15 After the assay is complete please reload tray Load Button on top of screen Exit the Envision software by closing the full envision software 16 Log out of your account
4. ion if you chose Tb LanthaScreen Repatest Went rden PAI start sty e ES eee H Bore 7 Right click on Well Type to reveal several different activities Measurement Kinetics Shaking Temperature Control 8 Select Measurement in the protocol window and select the correct filter set To do this select the small icon to the right of the drop down assay list circled Valid Measurements are indicated with an asterix next to them rol giota roes CE Sint pee ecen i a 9 A list of preloaded read protocols will appear on the left side of the screen From the list on the left click measurement technologies and method of choice If your measurement is not available in the list you can create you r own by first duplicating an existing measurement Fle Et vien Tob Actions Heb 9 0 32 Bak mwe oa tat F Alte onan m n D Gerena vc os E GA mrut am face Lanes pm cu ae taajaan w us 4 Aenm tase Lear Openu LANES OBA Du unc soc ess w Ensen X Sut u al ne a w Eraser Lthasoeen T BADOE Suk LANEDR Cu ane tansan w ese Easpun ie DNIE Sue 1 LANCEDELEIA Cus Nana soc sss Euicpumeis mo Emn spago L028 Copy if Larthasaoen Eu Last DOANDEN S01 LANCE DEFIA Dual nme 6c 65 Eurepun tS th Erion feo Stem Letrasonen Tolar Garer DANIE 1 LMI OELFLADual one remem oesi mo Esn BBL Anza enacts DeWsDEs Sue GOERS Wana saps osfirm e Ein 924
5. lch Lame 394 Erten pear cence tes Jeret 2 A e eee Gren scans Tests oat rite Recrde Br Mans Protocol oats C _ ow Ertan one Dom a tne noes am cana T twaen Prosoma 32 cortar i SF quel ean test CL 04 Costar t Eriten Presan New 204 Gostar i Ewan Fedatin Baton 3 costar 1 Ene Fah tano Us opinias Conwguy sagens 87 Enion Focrecen Flate Ooi metin IDA Ganire PEAY t BNIPEDIL B5PEAF No Erien 00 escabin obne IW Costar L ILZO ITAU FI No Crista sigan 10 12 12 AM Pesta 1M censa t anjon 3857 Ho tae rieaegit 13 54 15 24 Crouet 234 Costar t eigen 2392661 No Erten Waan tsaa PH Copy of Predictor 204 Costar L 8 9 3012 4 30 39 PM No Evaon B2011 HSPA me S6 Genera i na No Eritaca sjajan aio an mestan IW Costar t th fo Eriten siess Latham Eubndrg Jane Nanci Corra Ly FACE 377 L Saa Ilte o Enean SAN Lass A 4 The new protocol will open immediately 5 From the Protocol menu near the center of the screen go to General Settings and enter your plate type z Mention Nascuenant made Lat aun Murter of runs a ij ut settings iatt aN amem Wele meson Grou Hemrenents Mates a B Generat hei ee ey Ln retata pte r eee Use general groper heh Gd anyer beigt T Use measurement hajt defined n label Gd Frond messreent Fent r E 6 Now select Well Selection A new screen will appear with a plate schematic allowing you to use the drop down tool bar to define your wells as needed Controls or Blanks for auto subtract
6. urimesrence 1A C DEFIA Tre resoherd Foros D o Rarer ES Aes Sarcke aot stih AG ese sennae i Aer in 10 A new window will open for with the measurement parameters 11 For kinetic runs choose number of repeats and time intervals per repeat X e peete Neer Caneel ms Tb LanthaScreen s E steel General seta 9 7 S2e8RF 2 Measurement umr EA osoutsoninos liaa b Srei Labs ete maed nt Optica B E Wels selection Gra somatic e Ss esc as Puak 3 Catana openers Fates kiant a YUk test arte est Laneta ras Muter st siwe repes e Frente He ra Prestolue Bottom BE serriterepestenh ko Thala Simei Te rth Breasts 15 Asay eeamples h C Walac protocols E Barcode satros UG taste serra B Racy n 12 When finished select start button at the top of the screen It will take you through the optimization wizard The optimization protocol standardizes the sample height and gain for your specific assay 13 The assay wizard will open automatically Select optimize existing protocol then select Next Select the protocol you want to optimize and follow the directions on the screen E Uber protocols Tgp enrascccn F O Phic open D aren som A rhonercerce mterty Test Latha Fas m E assay evampes 1 rab oroconcts Idle Sclctprotucal uad protocole maod wth andes Mr button ta start the 2002p Welcome to the Assay St
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