Agilent 500 Ion Trap LC/MS with the Agilent 1200 Infinity Series
Contents
1. 23 45 6 7 8 ON Figure 4 Switch settings to initiate with default settings 3 Follow the instructions that came with the G1369B LAN card to install the card into the pump or autosampler Agilent 1200 Infinity Series LC to Agilent 500 lon Trap LC MS Set Up Guide Step 1 Install the LAN card into the Agilent 1200 Infinity Series 4 Connect one end of the supplied LAN crossover cable to the LAN card Connect the other end to the LAN connector on the PC that controls the Agilent 500 Ion Trap LC MS Figure 5 Crossover cable connected to the LAN card installed in the Agilent 1200 Infinity Series pump module The LAN card has been set to the default setting so it will use the default IP address of 192 168 254 11 Agilent 1200 Infinity Series LC to Agilent 500 lon Trap LC MS Set Up Guide 3 Step 2 Configure the network on the PC to work with the LAN card Step 2 Configure the network on the PC to work with the LAN card 1 On the control PC click Start gt Control Panel gt Network Connections gt Local Area Connection gt Properties gt Internet Protocol TCP IP Network Connections e Control Panel File Edit View Favorites Tools Help Gk BD Pluh H raiders Fi Q Bacl x J gt Search gt Folders abe Address Network Connections Name See Also Administrative Tools 4p Windows Update Automatic Updates Help and Support Bluetooth Configuration Folder Options Connect using B Broadcom2. 500 lon Trap LC MS Set Up Guide Step 1 Install the LAN card into the Agilent 1200 Infinity Series Install the G1369A LAN interface card into the LC detector module If a detector is not present install the LAN interface card into the pump module or the autosampler The pump module is preferred over the autosampler For G1315C D DAD SL detectors The LAN interface for the G1315C D DAD SL is built into the module main board No LAN interface card installation is needed but you need to make sure the switches are properly set 1 Set the switches on the module main board so that switches 7 and 8 are ON or UP See Figure 2 Refer to the Agilent 1200 Series Diode Array and Multiple Wavelength Detector User Manual p n G1315 90012 for more information ON 23 4 5 6 7 8 Figure 2 Switch settings to initiate with default settings for the G1315C D DAD 2 Continue at step 4 on page 3 Agilent 1200 Infinity Series LC to Agilent 500 lon Trap LC MS Set Up Guide 1 For all other LC modules 1 Make a note of the MAC address of the LAN card See for Figure 3 for location Switches DG l ee ce ee a se oe ee MAC Label c C66 can oe T us A 7 A ii f z i s E i Board information tantan imi 8 ai BGM ay E 20 0077 i ow gs Figure 3 LAN card details 2 Set configuration switches 5 and 6 to ON so that the card initiates with default settings See Figure 4
3. Access Point Dual Channel Iface 359008 Selected Modules 1100 1200 System 1100 1200 Access IP 192 168 254 11 Figure 15 1100 1200 System listed as a configured module d Click OK Agilent 1200 Infinity Series LC to Agilent 500 lon Trap LC MS Set Up Guide Step 4 Connect the Agilent 500 lon Trap LC MS to the Agilent 1200 Infinity Series 1 Connect one end of the supplied remote cable to the REMOTE connector on the back of the autosampler See Figure 16 Figure 16 REMOTE connector on the back of the autosampler 2 Connect the other end of the remote cable to the User I O port on the back panel of the Agilent 500 Ion Trap LC MS See Figure 17 HUNINOUTUOUUUOUUUEOHQROUAQEUUUEUHUEUA HNIUNIUUHUAUHUAUUUAUUEUUHGUAUUULAUHOLUE Cee RAT Renee 9 isee Can 3 oe 10 Pa sostat Figure 17 User 1 0 port on the back panel of the LC MS Agilent 1200 Infinity Series LC to Agilent 500 lon Trap LC MS Set Up Guide 9 Step 5 Configure the Agilent 500 lon Trap LC MS 1 Open the Agilent MS Workstation toolbar 2 Right click the right side of the toolbar then in the shortcut menu select Enable Disable Instrument Modules See Figure 18 Enable Disable Instrument Modules Instrument Modules currently enabled Instrument Modules currently disabled Select instrument modules you wish to disable Select instrument modules you wish to enable Quad Mass Spec 2002 Micro GC 2003 Micro GC gt gt Disable gt gt 212 LC Solvent
4. Nettreme 57xx Gigabit C This connection uses the following tems Fonts v Chent for Microsoft Networks Game Controllers 7 Fie and Printer Sharing for Microsoft Networks 5HP 3D DriveGuard MEELIS BHP Quick Launch Buttons PHP Wireless Assistant De Control Pane 4 Gaintel R GMA Driver for Mobile My Network Places Baas Internet Options G My Documents Transmission Control Protocol Intemet Protocol The defaut te Ro ed eae posal owe sterling gg gore Keyboard a M if C Show icon in notification area when connected Mal Z Notify me when this connection has limted o no connectivity SEE Network Connections POS re MOC UTI Figure 6 Path to open the Internet Protocol TCP IP Properties dialog box 2 In the Internet Protocol TCP IP Properties dialog box for IP address set the first 3 segments to be the same as the LAN card that is 192 168 254 3 Set the last segment of the IP Address to something different from the LAN card such as 10 See Figure 7 Internet Protocol TCP IP Properties General You can get IP settings assigned automatically if your network supports this capability Otherwise you need to ask your network administrator for the appropriate IP settings Obtain an IP address automatically Use the following IP address IP address 192 168 254 10 Subnet mask 255 255 255 0 Default gateway DNS se ess au Use the following DNS server addresses
5. Preferred DNS server Altemate DNS server Figure 7 Set IP address 4 Agilent 1200 Infinity Series LC to Agilent 500 lon Trap LC MS Set Up Guide 4 Click Advanced then in the Advanced TCP IP Settings dialog box a Click the IP Settings tab then check that the settings match those in Figure 8 x x Advanced TCP IP Settings IP Settings DNS WINS Options IP addresses IP address Subnet mask 192 168 254 10 255 255 255 0 Renove Defauk gateways Gateway Metric V Automatic metic Figure 8 IP Settings b Click the DNS tab then check that the settings match those in Figure 9 Advanced TCP IP Settings 3 IP Settings DNS WINS Options DNS server addresses in onder of use a The following three settings are applied to all connections with TCP IP enabled For resolution of unqualified names Append primary and connection specific DNS suffixes Append parent suffixes of the primary DNS suffix Append these DNS suffixes in order l DNS suffix for this connection cb varianinc com V Register this connection s addresses in DNS C Use this connection s DNS suffix in DNS registration Figure 9 DNS settings Agilent 1200 Infinity Series LC to Agilent 500 lon Trap LC MS Set Up Guide Inr l Arn t h Fa D f M da OI k on tne ru TO work with the LAN card c Click the WINS tab then check that the settings match those in Figure 10 Advanced TCP IP Settings
6. Agilent 500 lon Trap LC MS with the Agilent 1200 Infinity Series Set Up Guide Step 1 Install the LAN card into the Agilent 1200 Infinity Series 1 Step 2 Configure the network on the PC to work with the LAN card 4 Step 3 Install and set up ChemStation Software 7 Step 4 Connect the Agilent 500 lon Trap LC MS to the Agilent 1200 Infinity Series 9 Step 5 Configure the Agilent 500 lon Trap LC MS 10 Use this guide to set up an Agilent 1200 Infinity Series to work with an Agilent 500 Ion Trap LC MS Before you begin make sure you have Sync cable p n G3932 60001 G1398B LAN Card Kit p n G1369 60002 ChemStation Software MS Workstation Software Refer to the ChemStation and MS Workstation installation guides for system requirements fee Agilent Technologies 500 Ion Trap LC MS 1200 Infinity Series Set Up Workflow Configure PC Install MS network for LAN Workstation and communication ChemStation on PC Install LAN card in 1200 Infinity module Configure 1200 Infinity Series in ChemStation Enable LAN communication Connect 1200 Connect serial cable module to PC via 4 from 1200 module to crossover cable J9 port on LC MS Configure 500 MS Disable all Open MS Clear the AutoStart modules except k Workstation Module box 500 MS System Control 500 lon Trap LC MS now synchronized with 1200 Infinity Series Ready to Use Figure 1 Set up overview 2 Agilent 1200 Infinity Series LC to Agilent
7. Delivery System 216 LE nanoLC System 3800 GC 500 MS Mass Spec 800 Interface Box 8200 AutoS ampler 9001 Solvent Delivery System 9002 Solvent Delivery System 9010 Solvent Delivery System lt lt Enable lt lt 9012 Solvent Delivery System 9050 U Vis Detector 9100 AutoS ampler 9200 Prospekt 9300 AutoS ampler ADC Board OK Cancel Figure 18 Enable Disable Instrument Modules dialog box 3 Disable all modules except for Quad Mass Spec To disable a module select the module from under Instrument Modules currently enabled then click Disable 4 Open the System Control window 5 Check that the QUAD icon is not in the AutoStart Module box See Figure 19 Agilent 1200 Infinity Series LC to Agilent 500 lon Trap LC MS Set Up Guide Step 5 Configure the Agilent 500 lon Trap LC MS T System Control Configuration per ile Edit Inject Automation Recalculate Instrument Windows am Configuration Sep 29 11 56 24 System Control Copyright 1989 2009 Yarian Inc Figure 19 System Control Configuration window Agilent 1200 Infinity Series LC to Agilent 500 lon Trap LC MS Set Up Guide 11 Step 5 Configure the Agilent 500 lon Trap LC MS 12 6 Configure the Sync signals for the Agilent 500 Ion Trap LC MS a Click Configuration gt Sync signals System Control 325 MS Waiting for Injection of Sample File Edit Inject Automation Recalculate Instrument Windows Help He EJE gt Pcl Fel 20
8. gt a Waiting az QUAD 42 Ready a scheduled mrm s tmrm 07_15_10 9 29 2010_12 1 9 22 Sample 3_Method1 Toots f Reports He 45 3 a w Fal Instrument 1 Sep 29 12 21 40 Ins 1 SyncOut READY Figure 20 Sync signals command b Configure the Sync Signals as shown in Figure 21 Agilent 1200 Infinity Series LC to Agilent 500 lon Trap LC MS Set Up Guide Sync Signals Start Out Signal Ready Out Signal Enabled iri N MV Enabled iri P When the instrument starts an acquisition it closes Wiring The instrument changes the contact between pins the contact between pins 4 and 5 on Row 2 Column c 4 and 5 on Row 1 Column C of the IO connecter oe a eTa Ready closes contact when it is ready to acquire data and reverses it Ready opens contact when it is not ready Ready In Signal Start In Signal l Enabled witing The instrument senses the readiness of another M Enabled C VarianGC Cable 03 937179 01 GC QuadMS device through Pins 2 and 3 at Row 2 Column A of the I0 Connector When the state between these Generic Cable 03 937413 01 Generic QuadMs or pins is sensed open or closed depending upon Win Generic I0 Board Assembly CUBO6 00047 which radio button is selected the device is 9 considered ready Contact open ready Looking for anup edge If the instrument is ready to acquire data closing the contact between Pins 2 and 3 at Row 1 Column amp Contact closed ready C Looking fora down edge of the IO co
9. nnector will start an acquisition Test Syne Signals Start In Signal Wiring CONTACT CLOSURE INPUT 1 ADD JUMPERS BETWEEN PINS 1 AND 2 AND PINS 3 AND 4 ANALOG TO 5 a DIGITAL 3 CONTACT CLOSURE CONVERTER CIRCUIT GROUND CABLE SHIELD GROUND ROW 1 COL A 37 PIN DSUB CONNECTOR ANALOG GND CHASSIS GND Alternate wiring methods Generic I0 Board Assembly CUB06 00047 Analog Digital Contact Closure C Open Collector Figure 21 Sync Signals settings The Agilent 500 Ion Trap LC MS is now ready to run sample analysis and collect MS data with the Agilent 1200 Infinity Series Refer to the Agilent 500 Ion Trap LC MS with the Agilent 1200 Infinity Series Quick Start Guide for more information on control of the Agilent 1200 Infinity Series with the Agilent 500 Ion Trap LC MS Refer to the operation manuals and user guides for both the Agilent 500 Ion Trap LC MS and the Agilent 1200 Infinity Series for operation and maintenance Agilent 1200 Infinity Series LC to Agilent 500 lon Trap LC MS Set Up Guide 13 www agilent com In This Book This guide describes how to set up the Agilent 1200 Infinity Series to be used with the Agilent 500 Ion Trap LC MS and the MS Workstation software Agilent Technologies Inc 2010 2015 Revision Al February 2015 9300032300 fhe Agilent Technologies
10. strument 1 cation C Chem32 CORE 1 Remote Phone Log gt R Instrument 1 online gt PN Spider Soltare ms Workstation IQT Report gt ReadMe txt I Agilent BootP Service emStation a Ss Scheduler Figure 12 Path to open Configuration Editor 3 Click Configure gt Instruments to open the Select Instrument dialog box 4 For Instrument Type select Modular Systems Classic then click OK See Figure 13 Select Instrument Insteument Type LC Data Analysis oriy Modular LC System Instrument Name linstrument 1 f gt Intial Screen Window Size C Noma icon Full screen Lox coca eof Figure 13 Select Modular Systems Classic 5 In the Instrument 1 Device Configuration dialog box a Click 1100 1200 Access b In the 1100 Modular System dialog box for IP Address type 192 168 254 11 then click OK Agilent 1200 Infinity Series LC to Agilent 500 lon Trap LC MS Set Up Guide Instrument 1 Device Configuration Modular LC System Classic Modules C Identify by Host Name Identify by IP Address IP Addess 192 168 254 11 OK Cancel Help Figure 14 Set IP address for the LC c Check that 1100 1200 System appears in the Instrument 1 Device Configuration dialog box to verify that the PC has successfully connected to the Agilent 1200 Infinity Series Instrument 1 Device Configuration Modular LC System Classic Modules Camon Instr Ctrl
11. ud R paeem IP Settings DNS WINS Options WINS addresses in order of use IF LMHOSTS lookup is enabled it applies to all connections for which TCP IP is enabled Enable LMHOSTS lookup Import LMHOSTS NetBIOS setting Default Use NetBIOS setting from the DHCP server If static IP address is used or the DHCP server does not provide NetBIOS setting enable NetBIOS over TCP IP Enable NetBIOS over TCPAP Disable NetBIOS over TCP IP Figure 10 WINS settings d Click the Options tab then check that the settings match those in Figure 11 Advanced TCP IP Settings PR IP Settings DNS WINS Options Optional settings TCPAP fikenng Description TCP IP filtering allows you to control the type of TCPAP network traffic that reaches your Windows computer Figure 11 Options settings 6 Agilent 1200 Infinity Series LC to Agilent 500 lon Trap LC MS Set Up Guide Step 3 Install and set up ChemStation Software 1 Install the ChemStation software onto the control PC 2 Configure the 1200 Modules a Click Start gt All Programs gt Agilent ChemStation gt Configuration Editor Configuration Editor s Remote Assistance O Windows Media Player a ti Instrument 1 online 3 Windows Messenger Windows Movie Maker B Add Instrument p Y Add Instrument E Network Associates gt nfiguration Edror B ment a WordPad GD Sentech gt Ge In
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