Progesterone Receptor (PR) [16]
Contents
1. Progesterone Receptor PR 16 Concentrated and Prediluted Monoclonal Antibody Control Number 902 424 061615 Catalog Number CM 424 A C OAR 424 T60 Description 0 1 1 0 ml concentrated 60 tests prediluted Dilution 1 50 1 100 Ready to use Diluent Van Gogh Yellow N A Intended Use For Research Use Only Not for use in diagnostic procedures Summary and Explanation Progesterone Receptor PGR content of breast cancer tissue is an important parameter in the prediction of prognosis and response to endocrine therapy 5 Studies have shown PGR clone 16 is directed against the human progesterone receptor molecule 1 5 A prokaryotic recombinant protein corresponding to the N terminal region of the A form of human progesterone receptor was used as the immunogen Antibody characterization studies demonstrated that PGR clone 16 reacts with both A and B forms of human progesterone receptor by Western blotting procedure 4 Source Mouse monoclonal Species Reactivity Human others not tested Clone 16 Isotype IgG1 Total Protein Concentration 10 mg ml Call for lot specific Ig concentration Epitope Antigen Progesterone receptor Cellular Localization Nuclear Positive Control Progesterone Receptor positive breast carcinoma Known Applications Immunohistochemistry formalin fixed paraffin embedded tissues Supplied As Buffer with protein carrier and preservative Storage and Stability Store at 2 C to 8 C Do not2. de Stainer Protocol Editor should be programmed as follows Protocol Name PR Protocol Template Description Ms HRP Template 1 Dewaxing DS Option DS Buffer Antigen Retrieval AR Option AR2 low pH 103 C Reagent Name Time Temp PR 30 min 25 C Biocare Medical LLC 4040 Pike Lane Concord CA 94520 coal Tel 800 799 9499 www biocare net Technical Note This antibody has been optimized for use with Biocare s MACH 4 Universal HRP Polymer Detection and ONCORE HRP Detection Other Biocare polymer detection kits may be used however users must validate incubation times and protocols for their specific application Use TBS for washing steps Limitations This product is provided for Research Use Only RUO and is not for use in diagnostic procedures Suitability for specific applications may vary and it is the responsibility of the end user to determine the appropriate application for its use Precautions 1 This antibody contains less than 0 1 sodium azide Concentrations less than 0 1 are not reportable hazardous materials according to U S 29 CFR 1910 1200 OSHA Hazard communication and EC Directive 91 155 EC Sodium azide NaN used as a preservative is toxic if ingested Sodium azide may react with lead and copper plumbing to form highly explosive metal azides Upon disposal flush with large volumes of water to prevent azide build up in plumbing Center for Disease Control 1976 National Institute of Occupati
3. onal Safety and Health 1976 6 2 Specimens before and after fixation and all materials exposed to them should be handled as if capable of transmitting infection and disposed of with proper precautions Never pipette reagents by mouth and avoid contacting the skin and mucous membranes with reagents and specimens If reagents or specimens come in contact with sensitive areas wash with copious amounts of water 7 3 Microbial contamination of reagents may result in an increase in nonspecific staining 4 Incubation times or temperatures other than those specified may give erroneous results The user must validate any such change 5 Do not use reagent after the expiration date printed on the vial 6 The SDS is available upon request and is located at http biocare net Technical Support Contact Biocare s Technical Support at 1 800 542 2002 for questions regarding this product References 1 Qiu J et al Effect of delayed formalin fixation on estrogen and progesterone receptors in breast cancer a study of three different clones Am J Clin Pathol 2010 Nov 134 5 813 9 2 Arihito K et al Comparison of evaluations for hormone receptors in breast carcinomas using two manual and three automated immunohistochemical assays Am J Clin Pathol 2007 Mar 127 3 356 65 3 Press M et al Comparison of different antibodies for detection of progesterone receptor in breast cancer steroids Steroids 2002 Aug 67 9 799 813 4 Mote P e
4. t al Detection of progesterone receptor forms A and B by immunohistochemical analysis J Clin Pathol 2001 Aug 54 8 624 30 5 Bevitt D et al New monoclonal antibodies to oestrogen and progesterone receptors effective for paraffin section immunohistochemistry J Pathol 1997 Oct 183 3 228 32 6 Center for Disease Control Manual Guide Safety Management NO CDC 22 Atlanta GA April 30 1976 Decontamination of Laboratory Sink Drains to Remove Azide Salts 7 Clinical and Laboratory Standards Institute CLSI Protection of Laboratory Workers from Occupationally Acquired Infections Approved Guideline Fourth Edition CLSI document M29 A4 Wayne PA 2014 Page 1 of 1 Fax 925 603 8080
5. use after expiration date printed on vial If reagents are stored under conditions other than those specified in the package insert they must be verified by the user Diluted reagents should be used promptly any remaining reagent should be stored at 2 C to 8 C Staining Protocol Recommendations manual use Peroxide Block Block for 5 minutes with Biocare s Peroxidazed 1 Pretreatment Solution Diva Pretreatment Protocol Retrieve sections under pressure using Biocare s NxGen Decloaking Chamber at 110 C for 20 minutes alternatively follow the recommendations in the Decloaking Chamber User Manual if using a different model Protein Block Incubate for 15 minutes at RT with Biocare s Background Punisher Primary Antibody Incubate for 45 minutes at RT Probe Incubate for 10 minutes at RT with a secondary probe Polymer Incubate for 20 minutes at RT with a tertiary polymer Chromogen Incubate for 5 minutes at RT with Biocare s DAB OR Incubate for 5 7 minutes at RT with Biocare s Warp Red Counterstain Counterstain with hematoxylin Rinse with deionized water Apply Tacha s Bluing Solution for 1 minute Rinse with deionized water Protocol Recommendations OQNCORE Automated Slide Staining System OAR424 is intended for use with the ONCORE Automated Slide Staining System Refer to the ONCORE Automated Slide Staining System User Manual for specific instructions on its use Protocol parameters in the ONCORE Automated Sli
Download Pdf Manuals
Related Search