YSI Pro Series Instrument
Contents
1. in this solution during storage so it is important to ensure it is sealed on properly so the solution doesn t evaporate or leak Note Refer to Page 58 68 in the YSI Professional Plus User Manual for more detailed Care Maintenance and Storage instructions Viewing Saved Data e Press the File button e Scroll down with arrow keys until View Data is highlighted press Enter e Scroll down with arrow keys until you reach your site that will be highlighted press Enter To Save in Site e Scroll down with arrow keys until Site is highlighted press Enter 28 Select the site you wish to view data from or select All Sites using the arrow keys Scroll up to show data is highlighted press enter Use left and right arrow keys to view data from different sensors Record all of the data in your field book Press the Esc button to return to the main screen Downloading Data from the YSI pro plus to a computer Installing Data Manager program and USB drivers to a Computer Data Manager needs to be installed on the computer before you try to connect the instrument to your computer First install Data Manger then connect the communications saddle to the computer and lastly connect the saddle to your Pro Plus Refer to the Data Manager Readme file on the CD for detailed installation instructions The instrument comes with a CD put it in your computer the software can also be downloaded from Y2. pH sensor is now enabled To enable the ORP sensor e Press the Probe key e Highlight Set up with arrow keys and press Enter e Scroll down using the arrow keys to ISE2 and press Enter e Use the arrow keys to highlight ORP and press Enter e The ORP sensor is now enabled and there should be a checkmark next to ORP All sensors should now be enabled Note If a sensor is enabled but isn t connected to the instrument the display will show a Or before the units Selecting Different Units You can select which units the different parameters will be reported in e g mg L You should not need to do this often because we will be using the default units programmed into the meter when you receive it e Press the Probe button e Scroll down with arrow keys until Display is highlighted press Enter e Scroll down until the parameter you wish to access is highlighted press Enter Professional Ac e Another menu will appear use the arrow keys to select which unit you wish to use There will be a check mark in the box of any units you have selected to use Note Some parameters can be reported in multiple units e g DO others e g temperature cannot Installing Sensors When connecting the sensors to the instrument there are three things to remember 1 Ensure sensor and sensor port are clean and dry 2 Ensure the sensor is properly aligned wi
3. pro plus to the computer iria dis 29 Downloading water quality data to the COMputeT oocccccocccncnnconcnnnnnnonanononarononnnnnnonaconenaronenanos 30 GLP Good Laboratory Practice ici in 30 PRE TON laa O O 5 o II O AA 31 Please see the video associated with this manual for visual demonstrations of the procedures presented in this manual The video times associated with the different manual sections are as follows 0 05 Introduction pg 4 4 17 Installing Batteries pg 4 5 26 Basic Functions pg 5 7 26 Setting up Enabling Sensors pg 7 12 01 Installing Sensors pg 9 12 33 Bulkhead pg 10 13 43 Installing the pH sensor pg 11 16 32 Installing the Dissolved Oxygen Sensor pg 11 20 49 Installing the Oxidation Reduction Potential ORP sensor pg 12 22 24 Installing the conductivity temperature sensor pg 12 24 29 Connecting the cable to the instrument pg 14 24 54 Short Term Storage pg 27 26 10 The Autostable Function pg 24 28 23 Calibration pg 14 28 28 Conductivity Temperature pg 15 38 14 Dissolved Oxygen pg 16 42 48 pH pg 17 52 08 ORP pg 18 56 31 Taking Measurements pg 25 and Storing Data pg 21 59 26 Maintenance pg 25 59 45 Storing data pg 21 Introduction This YSI hand held meter is a piece of monitoring equipment that measures water quality parameters What are Parameters Parameters are a measurable factor The sensors are used to collect information about these different parame
4. tissue to remove excess Krytox e Install the probe into port 1 of the bulkhead by pushing the sensor into the port until only one O ring remains visible e Twist the grey portion of the sensor clockwise to screw the sensor into place and finger tighten Installing the Oxidation Reduction Potential ORP sensor e Putona pair of protective gloves e Take the ORP sensor out of its box and remove it from the small bottle of electrode storage solution Use the second white cap without the hole to close the bottle of solution e Lubricate the sensor O ring with a small dab of Krytox from the small white tube just to the point that it is shiny Do not over lubricate If you apply too much o ring lubricant use a lens cleaning tissue to remove excess Krytox e Push the ORP sensor into port 2 on the bulkhead until only one o ring is visible e Twist grey portion of the sensor clockwise to screw in the sensor e Finger tighten Installing the Conductivity Temperature Sensor e Take the Conductivity Temperature Sensor out of its box e Lubricate the sensor O rings with a small dab of Krytox from the small white tube just to the point that it is shiny Do not over lubricate If you apply too much o ring lubricant use a lens cleaning tissue to remove excess Krytox 12 e Line up the sensor connector with the connector inside the port You might have to turn the sensor slightly inside the port to get the alignment correct e Once the co
5. Logging data at specific intervals e Press the System button e Use the arrow keys to scroll down until Logging is highlighted press Enter e Highlight Continuous Mode to enable it press Enter There will be a checkmark in the box to show continuous mode enabled e Use arrow keys to scroll down to Interval is highlighted press Enter e Use the arrow keys to select the timings pressing Enter after each number is selected Professional 23 e Use the arrow keys to scroll down until ENTER is highlighted press Enter e Press the Esc button to take you back to the main screen e When your back on the main screen Log One Sample will be changed to Start Logging according to the time interval you have selected press Enter to begin logging data Professional As e Once ona continuous log the box will change to Stop Logging e When finished press Enter to stop the instrument from collecting data The Autostable Function This meter has a function called autostable that will alert you when a parameter s reading is stable and ready to measure You will want autostable to be on for each parameter that you are measuring For example to turn on the autostable function on for pH e Press the probe button e Scroll down to Autostable and press enter e Scroll down to ISE1pH and press enter e You will now be at a screen where enable
6. SI s website at ysi com software Open My Computer Double click on the YSI CD picture Another screen will open up showing all of the files on the CD Double click on YSI Data Manager to begin installing Data Manager A box will open beginning installation follow the prompts it goes through Note It is important to install Data Manager on your computer before connecting the YSI meter to your computer These instructions may vary depending on what version of Windows you have on your PC Refer to the ReadMe file on the CD by double clicking the icon for more details Connecting the YSI pro plus to the computer A black plastic saddle and a USB cable are included with your instrument Insert the small end of the USB cable into the top of the saddle Insert the bigger end of the USB cable into your USB port on your computer If connecting for the first time Windows will likely prompt you to go through two Found Hardware Wizards in order to complete the installation Go through both these wizards when prompted The drivers must be installed before attaching the instrument to the saddle Otherwise DataManager will not recognize the instrument has been attached Attach the black plastic saddle to the instrument by first clicking in the top of the saddle to the back of your instrument oval shaped groove Align the grooves on the top and middle of the saddle 29 e Once aligned push down on the saddle and it should snap int
7. Step 13 The instrument will automatically recognize the standard value and will display it at the top of the screen Step 14 Highlight Accept Calibration and press enter to accept the second calibration point Step 15 Record After Calibration pH Reading in pH units and the post calibration pH millivolts on the calibration sheet This pH millivolt value should be should be between 165 mV and 180 mV below the pH 7 mV reading If this is not the case this could mean the probe needs to be cleaned Clean the probe according to the manufacturer s recommendations see section on page 25 of this manual on sensor maintenance and cleaning and then recalibrate If the pH millivolt reading is still not within the range specified above the reference solution inside the probe might be too old The sensor may need further maintenance or to be replaced The lifespan of reference solution inside pH sensors is only 1 5 2 years Step 16 Remove the calibration cup from the bulkhead and dispose of the pH 10 standard Rinse the sensors and calibration cup 3 times with de ionized water Step 17 If you have a third pH standard i e pH 4 follow the directions above to accept a third calibration point Otherwise press the Cal button to finish the pH calibration Oxidation Reduction Potential ORP You will need de ionized DI water and Zobell s solution Note Calibrate ORP after successfully calibrating pH Step 1 Prepare the Zobell calibration soluti
8. YSI Professional Series Water Quality Meter Instruction Manual Version 1 Contents E 4 Mt Set lcd 4 Baterista ars o EN 4 BASIC FUNCION Sist a 5 SAN o 5 Setting the TIM earn 6 Seting UP ENADIRE SENSO Sas 7 installing Sensor AA A 9 Me BUKRA S OE 10 COMME CHINE SEASON EN A 11 Cala CON asa 14 Supples required tor COMO NAAA do 14 Conductivity ana Temperature da 15 Dissolved OXY EEN a A AS Ad 16 O A E A oamnene tea 17 Oxidation Reduction Potential ORP cccooocccnnncnnnonacononacinonacicnonacononarinonanicnonarononarinonaninos 18 Corado NEE ae er arene aR oe E E Oo 20 SONDE Dat rd 21 Logging Gata at SOECITIC interval das 23 The Autostable FUNCTION Gs is ds 24 Taking Measurement o ta al e 25 MUECA eo POPE AAEN AE atone erates es 25 CONQUE Temperat Crispin a aiii 25 DIOR Pesada is 25 Maintenance and Store adds 25 RODIACE O ADE nicotina E S 25 cleaning Sen or PONS rnei a a a 26 cleaning SCMSONS a a a ia 26 SON Or OIE A A a a ON 27 SO Term tora SO N A EA NEOS eee ere Ree 27 LONE Term SOFIE Errian A E R 27 Conductvuity Temperatileinia di A O dt asedes 28 DiISSOIVE OXY BEN aia 28 O 28 OR oreo aia ate a cic a aa asap ee aaa erty cael asc as esac need ce eee 28 VIEWINS Save Data a 28 Downloading Data from the YSI pro plus to a COMpUteT ooccccocccnccnccnncnncnnonacononaronncnanononaronenarononos 29 Installing Data Manager program and USB drivers to a COMpuUteT cccoccccnccccnncncnnccnnnacnnnannnnnss 29 Connecting the YSI
9. ain date time for 2 minutes when batteries are out This is important if you intend to log data Basic Functions e System button will take you to the screen with all of the instruments basic settings e Use Esc whenever you would like to exit the current screen and return to the main screen Using the left arrow key will take you back to the previous screen instead of to the main screen e Probe button also referred to as sensor button will take you to the sensor menu Use this button to enable sensors and display units e Cal or calibration button opens the calibrate menu Use to calibrate all parameters except temperature e File button is used to view data and good laboratory practice glp files set up site and folder lists and delete data Note At any time the 2 button can be pressed to receive tips during operation Setting the date e Press system key e Use arrow keys to scroll down and Highlight Date time press Enter Professional s e Scroll down to Date press Enter e Use arrow keys to press on the numbers for the current day pressing Enter on each number you wish to select When finished scroll down with arrow keys until Enter is highlighted press Enter Professional Wr Setting the Time e Use arrow keys to scroll down and highlight Time press Enter Professional Ls e Use arrow keys to select numbers for the current t
10. and sensor information See the YSI manual for a more complete description about the information contained in the glp file References These instructions have been assembled and reproduced with modifications from YSI incorporated Professional Plus Quick Start Guide Revision B 2009 2 pages YSI incorporated Professional Plus Quick User Manual Revision D 2009 88 pages Please see the video associated with this manual for visual demonstrations of the procedures presented in this manual The video times associated with the different manual sections are as follows 0 05 Introduction pg 4 4 17 Installing Batteries pg 4 5 26 Basic Functions pg 5 7 26 Setting up Enabling Sensors pg 7 12 01 Installing Sensors pg 9 12 33 Bulkhead pg 10 13 43 Installing the pH sensor pg 11 16 32 Installing the Dissolved Oxygen Sensor pg 11 20 49 Installing the Oxidation Reduction Potential ORP sensor pg 12 22 24 Installing the conductivity temperature sensor pg 12 24 29 Connecting the cable to the instrument pg 14 24 54 Short Term Storage pg 27 26 10 The Autostable Function pg 24 28 23 Calibration pg 14 28 28 Conductivity Temperature pg 15 38 14 Dissolved Oxygen pg 16 42 48 pH pg 17 52 08 ORP pg 18 56 31 Taking Measurements pg 25 and Storing Data pg 21 59 26 Maintenance pg 25 59 45 Storing data pg 21 31
11. bles pH ORP These readings should happen quickly if it starts taking too long the sensor will most likely need cleaning Maintenance and Storage It is important to maintain the O rings so that water does not leak into the sensor ports or battery compartment Inspect the O rings every time you use the meter for dirt damage or if they are missing If the O rings are okay they should always be lightly greased Replacing O rings 25 e Use tweezers or a similar tool to remove the O ring from its groove Be careful if using objects like screwdrivers when removing the o ring Damage to the plastic channel where the o ring seats will cause a leak path e Clean the O ring groove for any excess dirt or lubricant if dirty clean with a lens cleaning tissue e Inspect the new O ring for any cracks nicks or imperfections e When installing new O rings ensure that your hands and work area is clean This is so no damage or dirt can affect the O ring while installing Any amount of dirt can cause a leak e Apply avery small amount of O ring lubricant to your thumb and index finger e Use this grease to lightly cover the O ring e Place the O ring into its grove making sure that it does not roll or twist e Use the leftover grease on your fingers to go over the outside of the O ring surface Note It is very important that no moisture grease or foreign material are allowed to contaminate the electrical connection of the probe during maintenance ca
12. ce e Rinse thoroughly and wipe with a wet paper towel before putting on a new membrane When sanding the shaft follow these few simple steps e Wet sanding disc with a small amount of clean water e Gently wrap it around the sensor shaft e Twist it a few times to lightly sand The goal is to simply sand off any build up without scratching or removing layers of the sensor itself e Rinse the sensor shaft with clean water and wipe with a lens cleaning tissue to remove any dirt that has been left behind e Finish off with rinsing the sensor tip with de ionized water The pH ORP sensor has glass bulbs that are very thin and are easily broken if any pressure is exterted on them The best way to clean them is by soaking them in a 1 1 bleach water solution for 10 20 minutes and then rinsing thoroughly in tap water if necessary If the pH ORP response must be restored refer to the YSI Professional Plus user manual for more detailed information Note Refer to Page 58 68 in the YSI Professional Plus User Manual for more detailed Care Maintenance and Storage instructions Sensor Storage Short Term Storage When not in use the cable comes with a grey rubber sleeve that goes over the probe guard Put the sponge and a cap full of water into it to maintain a moist air environment This is for short term storage less than a month Long Term Storage It is important when storing any of the sensors that they do not freeze while in storage This will
13. d standards with known levels of the chemical parameters we will be measuring We calibrate one sensor at a time The meter sensors are immersed in the standards one standard at a time and the verified concentration of the chemical that is present is input into the instrument This way the instrument can link the signal it is measuring to the proper known concentration Then when the instrument measures an unknown level of the same parameter in the lake or river being studied it uses the calibration information to calculate the concentration of the chemical in the water It is very important that the meter be calibrated before each use Calibrations of the different parameters measured by this meter can involve using one single standard a one point calibration or 2 standards a 2 point calibration Supplies required for calibration Calibration cup Standard approximate volume required De ionized DI water Conductivity standard 1413 uS cm 1 413 mS cm at 25 C pH 7 standard 14 pH 10 standard Zobell s solution Thermometer Calibration worksheet located at the end of this section Conductivity and Temperature You will need de ionized DI water and the conductivity standard 1413 uS cm 1 413 mS cm at 25 C Step 1 Rinse the sensors and calibration cup once with de ionized water Step 2 Rinse the sensors and calibration cup 3 times with conductivity solution 1413 uS cm 1 413 mS cm at 25 C Step 3 Place
14. damage them 27 Conductivity Temperature No special storage required can be stored dry or wet Just make sure the sensor is cleaned with the provided brush before and after storing for a long period of time Dissolved Oxygen This sensor should be stored in a dry state for long term storage 1 remove the membrane cap and thoroughly rinse with clean water 2 Blow it dry with compressed air or allow to air dry completely 3 Install a new membrane cap without electrolyte over the sensor to keep it dry and to protect the electrodes After storing the sensor for a long time it is important to condition the sensor by putting a new membrane with electrolyte solution on the sensor and then turning the meter on to allow the sensor time to stabilize pH It s always important to make sure this sensor does not dry out for both short term and long term storage Remove the sensor from the bulk head and seal the vacant sensor port Fill the original shipping bottle with buffer 4 solution and then submerge the sensor into the solution The sensor will remain in this solution during storage so it is important to ensure it is sealed on properly so the solution doesn t evaporate or leak The pH sensor is only good for 1 5 2 years with proper storage ORP Remove the sensor from the bulkhead and seal off the sensor port Fill the original shipping bottle with buffer 4 solution and submerge the sensor into this solution The sensor will remain
15. ime pressing Enter after each number Note Instrument should always be set to Mountain Standard Time e When finished scroll down with arrow keys until ENTER is highlighted press Enter Professional Ds Setting up Enabling Sensors The four sensors Conductivity Temperature Dissolved Oxygen pH and Oxidation Reduction Potential or ORP must be enabled one at a time as follows To enable Conductivity Temperature and Dissolved Oxygen sensors e Press the Probe key e Highlight Set up with arrow keys and press Enter Professional s e Using the arrow keys highlight Conductivity and press Enter e Enabled will be highlighted press Enter to enable it There will be a checkmark in the box to show the parameter is enabled e Press left arrow key to return to previous screen or Esc to the main screen e Enable the Dissolved Oxygen sensor by repeating the steps above The pH sensor will be installed in port ISE1 and the ORP sensor will be installed in port ISE2 To enable the pH sensor e Press the Probe key e Highlight Set up with arrow keys and press Enter e Scroll down using the arrow keys to ISE1 and press Enter e Highlight pH and press Enter e Scroll down to USA and press Enter e You will now be back to the setup menu and enabled will be checked e Press Escape to return to the system menu The
16. l Cell Constant 5 040 45 amp not more than 0 1 from historical value pH mV buffer 7 pL OMY 50 pH mV buffer 10 Pp 177 from pH 7 mV span b w pH4 7 mV P 165 to 180 mV span b w pH7 10 mV o 165to 180mV Maintenance Record Sensor Completed Y N N A Lo It not battery voltage Storing Data We will be storing data in site Use the name of the area you are sampling data from to name your site To set up the instrument to store data e Turn on instrument so that it is on the main screen e Place probe into sample e Move the probe around in the water until the readings stabilize and to release any air bubbles e Log One Sample will be highlighted on your instrument press Enter if e Use arrow key to scroll down until Site is highlighted press Enter on whichever place you wish to store your data e Use arrow keys to scroll down to Add New press Enter Professional e Use arrow keys to spell out the name of your site press Enter after each letter is selected Professional Ps 21 e When finished use arrow keys to scroll down to ENTER press Enter Note Use the keypad to make a new site name this option will not come up if Site list is disabled in the System menu e Use arrow keys to select name of site you wish to log data in press Enter professional Ds e The instrument will confirm the data is being logged 22
17. libration and cleaning Cleaning Sensor Ports Always make sure the ports are clean and dry If there is dirt in them use compressed air to clean them Cleaning Sensors Over time the conductivity temperature sensor will need to be cleaned Use the conductivity cleaning brush and a mild detergent to maintain accuracy of the readings Conductivity Cleaning Brush The dissolved oxygen membrane cap will need to be re placed over time The membrane should be changed if a Bubbles are visible under the membrane b Significant deposits of dried electrolyte are visible on the membrane c If the sensor readings don t stabilize While replacing the membrane examine the silver tip and shaft of the sensor to see if they are dull in colour If they are they will need to be sanded down with the circle sanding disc that was included with the membrane kit Only sand if it really needs it you may not need to every time you change the membrane 26 Remember to keep the sensor port clean and dry When sanding the silver tip follow these few simple steps e Dry the sensor tip completely with a lens cleaning tissue e Wet the sanding disk with a small amount of water and place it in the palm of your hand e Place the sensor tip directly on the sanding disk on the palm of your hand e Twist it in a circular motion three or four times to sand the gold should have a matte finish The goal is to sand off any build up and to lightly scratch the surfa
18. nnectors have met push the sensor in until you feel it all the way in It is normal to feel some slight resistance e Screw the nut at the base of the sensor clockwise with your fingers If the nut is difficult to turn STOP as this could mean its not threading properly If this happens unscrew and start again e Once the nut is finger tight use the silver tool to tighten it another 1 4 to Y a turn Do not over tighten the nut Installing Port Plugs For any ports that do not have sensors installed install a port plug Port Plugs are black plastic screws with two white o rings Port plugs protect the electronics inside unused ports from water damage When connecting port plugs put a thin layer of O ring lubricant Krytox on the o rings before screwing them in Finger tighten so that water cannot enter the bulkhead Using the Probe Guard Screw probe guard black tube with holes in to protect sensors while sampling 13 Connecting the Cable to the Instrument e Line up the slots with the bumps on the end of the cable e Push together firmly e Screw the outer metal ring until it is locked into place Calibration Calibration is an important process that helps the user ensure that the meter sensors are measuring properly and generating accurate water quality data We basically have to train the sensors so that they know how to measure the parameters we are interested in Calibration requires solutions calle
19. o place e Data Manager may open on its own If not open data manager Data manager will recognize the instrument is connected e The Home screen will appear where you can select various functions such as Retrieve Instrument Data Real Time Instrument Data Instrument Configuration or View Saved File Data Downloading water quality data to the computer e Click on your hand held instrument s serial number under the select Instrument heading e Under the Retrieve Instrument Data tab click Data files e The Retrieve Data page will open e Under Select Data check select all e Click Start e Click OK when data retrieval is complete e Click View Saved File Data tab e Click Data e Under Setup select your site from the drop down menu e Under Select Parameters max 6 click Barometer Specific Conductance Dissolved Oxygen ORP_1 pH_1 Temperature e Click View Data e Click Export e Click Browse and select where to save the data spreadsheet Excel file e Under Save as name your file e Click Export e Your data file will be in the location you specified For more detailed user information refer to the YSI Professional Plus User Manual or the ReadMe file on the CD Rom provided You can also visit www ysi com for more information on their products GLP Good Laboratory Practice 30 The glp file has detailed information about calibrations and diagnostic information about the sensors such as date time stamp calibration method
20. on according to the instructions on the bottle Open the bottle and add 125 mL of de ionized water to the dry powder The solution has a shelf life of 6 months once mixed Step 2 Rinse the sensors with a small amount of the Zobell solution 18 Step 3 Pour the Zobell solution into the calibration cup and immerse the pH ORP and temperature probes in the solution Step 4 Once the ORP reading stabilizes record the Before Calibration ORP Reading in millivolts on the calibration worksheet Step 5 Press the Cal button Step 6 Select ISE2 ORP press enter Step 7 The instrument will automatically recognize the standard and will display the value in millivolts at the top of the screen Check the recognized value against the following table to ensure that it makes sense ZOBELL SOLUTION VALUE MV a l l 3 J 4 4 0 0 T 7 50 EXT FEE Step 8 Highlight Accept Calibration and press enter Step 9 Record the After Calibration ORP Reading in millivolts on the calibration sheet 19 Calibration Sheet DateTime SS o y Checkedby oS S Time since last calibration A O Location e a A EE Used Reading a Barometric Pressure mmHg from meter ciar AAA eee w3 S Ss ssl Ouen EA ee ae Issolv X 7 Mie O pH AAA ooo AAA Oxidation Reduction Potential ORP Zobells CO A Comments YSI DETAILS VERY IMPORTANT TO FILL THIS CHART IN acceptable range Conductivity Ca
21. t to the point that it is shiny Do not over lubricate If you apply too much o ring lubricant use a lens cleaning tissue to remove excess Krytox e Push the pH sensor into port 1 on the bulkhead until only one o ring is visible e Twist grey portion of the sensor clockwise to screw in the sensor e Finger tighten Installing the Dissolved Oxygen Sensor Note Enable the DO sensor in the instrument program before physically installing the DO sensor into the bulkhead e Take the Dissolved Oxygen sensor out of its box e Install a cap membrane onto dissolved oxygen sensor as follows 1 If this is the first time the sensor is being set up remove the red cap from the tip of the dissolved oxygen sensor Thoroughly rinse the probe tip with distilled or de ionized water Find the membrane kit YSI 5913 If the galvanic oxygen sensor electrolyte bottle contains powder fill to neck with distilled water Select a new cap membrane from the box and fill it with electrolyte solution from the small bottle Do not touch the membrane surface Gently tap the side of the cap membrane to remove any air bubbles 11 6 Screw the cap membrane onto the sensor It is normal for a small amount of electrolyte solution to overflow during this step e Lubricate the sensor O ring with a small dab of Krytox from the small white tube just to the point that it is shiny Do not over lubricate If you apply too much o ring lubricant use a lens cleaning
22. ters This instrument measures 5 different water quality parameters Temperature Conductivity pH Oxidation Reduction Potential ORP Dissolved Oxygen DO A o ter Properly calibrated sensors that measure different water quality parameters are attached to one end of the meter The meter is used by submerging its sensors into a lake or river and it collects data from the water The data are then downloaded to a computer The meter should be calibrated before each use There are some advantages to using a meter over traditional water quality sampling methods like manually collecting samples and sending them to a lab For example the meter can collect a lot of data more frequently with less effort and at a lower cost than traditional sampling Of course there are disadvantages to using a meter as well The meter can only measure a few key parameters whereas a water sample that is collected and sent to a lab can be analyzed for a much broader suite of nutrients and contaminants Manufacturer name and model number of this meter YSI Pro Plus Initial Setup Battery Installation e Unscrew the four screws on the back of the YSI instrument e Remove plastic backing e Install 2 C size batteries e Replace plastic backing and screws Be careful not to overtighten the screws Overtightening could crack the battery cover and require a new one to be purchased Note changing the battery will re set the date time The instrument will maint
23. th the bulk head 3 Ensure to tighten sensors in place enough so that water doesn t get in but not to over tighten The Bulkhead The long cable with the black tube at the end i e the bulkhead is where the sensors plug in to To install the sensors you will need to unscrew the probe guard to reveal the bulkhead There are four ports on the bulkhead with red protectors that will hold four different sensors They are labelled DO Dissolved Oxygen CT Conductivity Temperature 1 and 2 Below is a table outlining which sensors go into which ports pH Oxidation Reduction Galvanic Dissolved Conductivity Potential ORP Oxyen sensor DO Temperature sensor CT Note A sensor must be installed in port 1 for proper operation of port 2 10 Connecting Sensors First remove the four red protective plugs from the sensor bulkhead Note The red protective plugs are for shipping only and cannot be used to protect the port when taking field measurements These plugs do not provide a water proof seal When installing sensors it is best to proceed in the following order port 1 DO port 2 CT Installing the pH sensor e Putona pair of protective gloves e Take the pH sensor out of its box and remove it from the small bottle of electrode storage solution Use the second white cap without the hole to close the bottle of solution e Lubricate the sensor O ring with a small dab of Krytox from the small white tube jus
24. the sensor into conductivity standard 1413 uS cm 1 413 mS cm at 25 C in the calibration cup and screw the cup closed The standard must cover all the holes in the conductivity sensor Ensure that the entire sensor is submerged and that there are no air bubbles in any of the holes by rotating the sensor gently The temperature sensor must also be completely submerged in solution Step 4 From the main screen record the Before Calibration Conductivity Reading in uS cm on the calibration worksheet Step 5 From the Calibrate menu highlight Conductivity and press enter Step 6 Select Sp Conductance specific conductance and press enter Step 7 Choose the units SPC uS cm and press enter Step 8 Scroll up to highlight Calibration value and press enter to input the value of the conductivity standard at 25 C in uS cm i e 1413 Use the arrow key until enter is highlighted and press enter The values of all the sensors will appear on the screen and will change as the readings stabilize Step 9 Once the temperature and conductivity readings have stabilized highlight Accept Calibration and press enter Step 10 You will now be at the main screen Record the After Calibration Conductivity Reading in the after calibration column of the worksheet Because of temperature effects on conductivity the post calibration reading will be different than the input standard value at 25 C i e 1413 uS cm if you are calibrating at a different tempera
25. tion Step 1 Rinse the sensors and calibration cup once with de ionized water and 3 times with pH 7 standard Step 2 Pour enough pH 7 standard into the calibration cup to immerse the pH bulb and the temperature sensor Step 3 Press the Cal button Step 4 Highlight ISE1 pH press enter Step 5 The message line at the bottom of the screen will show the instrument is ready for point 1 Step 6 The instrument will automatically recognize the standard value and will display it at the top of the screen Step 7 Once the pH and temperature readings stabilize record the Before Calibration pH Reading in pH units on the calibration worksheet This value will display under actual readings Step 8 Highlight Accept Calibration and press enter to accept the first calibration point Step 9 Record the After Calibration pH Reading in pH units and the After Calibration pH millivolts on the calibration sheet The pH millivolts value should be between 50 mV and 50 mV 17 Step 10 Remove the calibration cup from the bulkhead and dispose of the pH 7 standard Rinse the sensors and calibration cup 1 time with water and 3 times with pH 10 standard Step 11 Pour enough pH 10 standard into the calibration cup to immerse the pH bulb and the temperature sensor Step 12 Once the pH and temperature readings stabilize record the Before Calibration pH Reading in pH units on the calibration worksheet This value will display under actual readings
26. ture than 25 C This is normal 15 Step 11 Record the conductivity cal cell constant on the calibration worksheet You can find the conductivity cal cell constant by pushing the File button highlighting View GLP and pressing enter You will see a screen called Calibrate conductivity or Calibrate some other parameter Press the down arrow until you reach Calibrate conductivity Press the down arrow until you can see the Cal Cell Constant Record this Cal Cell Constant on the calibration sheet It should fall between 4 55 5 45 and not vary from the historical value more than 0 1 look back at previous calibration sheets to determine the historical value If it doesn t meet these criteria the calibration standard may be off Try another bottle of calibration standard A dirty conductivity sensor can also lead to bad calibrations Use soapy water along with the conductivity brush to properly clean the sensor If the cell constant is still off the sensor might need to be replaced Step 12 Record the temperature value in C from the meter on the calibration worksheet Step 13 Remove the calibration cup cap and add a thermometer to the solution and allow the reading to stabilize Record the value from the thermometer in C on the calibration worksheet The instrument and thermometer readings should be less than 2 C apart Step 14 Rinse all the sensors and cup thoroughly with DI water before calibrating the next parameter Dissolved Ox
27. will either be checked or not checked If it is not checked press enter to check the box and turn on the autostable function for pH e Press escape e Repeat this procedure for the DO ORP and Conductivity Temperature sensors e The DO and Conductivity Temperature sensors will keep the autostable function setting once enabled However the pH and ORP sensors must be enabled each time the instrument is turned off and back on 24 Taking Measurements Before taking measurements ensure all of ports have a calibrated sensor or a sensor plug installed as without these the connections are not waterproof Ensure that the probe guard is installed when taking measurements To collect the most accurate readings each sensor has a few different things to do while taking measurements Dissolved Oxygen e Place probe into the sample give a shake to release any air bubbles e Allow temperature readings to stabilize e Stir the probe in a 6 inch circle per a second e Once values plateau and stabilize record this measurement e The DO reading will drop over time if stirring is ceased When taking a DO sample in fast flowing water place the probe with the flow of water If taking a DO sample in an aeration tank basin point probe upwards so it s facing the sky This is so air bubbles don t burst directly on the sensor causing a flaw in the measurement Conductivity Temperature Place probe into the sample give a shake to release any air bub
28. ygen You will need de ionized DI water Step 1 Add a few mL of de ionized water and the sponge to the clean calibration cup Step 2 Make sure there are no water droplets on the dissolved oxygen sensor membrane or on the temperature sensor Do not touch the dissolved oxygen sensor membrane Step 3 Screw the calibration cup onto the bulkhead engaging a few threads so air can diffuse into calibration cup Do not tighten the cup completely onto the bulkhead Be sure the dissolved oxygen and temperature sensors are not submerged in water Step 4 Wait 5 to 15 minutes until the air in the storage container has completely saturated with water When the reading is stable record the Before Calibration Dissolved Oxygen Reading in mg L and in on the calibration worksheet Step 5 Press the Cal button Step 6 Use the arrow keys to highlight DO press enter 16 Step 7 Highlight DO L L stands for Local press enter Step 8 When the reading is stable highlight Accept Calibration and press enter The reading should now be very close to 100 Step 9 You should now be back at the main screen Record the After Calibration Dissolved Oxygen Reading in mg L and on the calibration worksheet Step 10 Record the barometric pressure in mmHg on the calibration worksheet Step 11 Empty the calibration cup and rinse with DI water pH You will need de ionized DI water pH 7 standard and pH 10 standard Note pH requires a 2 point calibra
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